Bhi12G000503 (gene) Wax gourd (B227) v1

Overview
NameBhi12G000503
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionGluconokinase
Locationchr12: 17914972 .. 17919018 (+)
RNA-Seq ExpressionBhi12G000503
SyntenyBhi12G000503
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAAAAATAAGGGCCAAATTGGTAATTTTAACAATAGATATTAATTTTGTTCTCGTCTTCAATTCTCAACTGCCATCTCTCACCACTTTGCCCAAAAATTTCATCATTCTCTGTCCATCACCTGGCATAAGGATTCTAGCATCCAAATTTGAATGGTATTCTATTCGATTCTTCAATTTCAGGTCTCATAATGGTAAGTTTCATTCAAAATTTGAGTTTATTAATCTCCGATTCTTCTTCTCATTCAAGCTATGGCGATCGTGCTCATGGGCGTCTGCGGTTCTGGAAAATCGTAAGTCTCCTCTCACTCTCTCCGACCTATGTCGATTCTTCGTCTTCTCTGAATATATCGACTTTGTAAATCGATCGATTGATTGATTCAATTTAGAGTCTAGTAGTGGAGAAATTAGTTAGTTATCAGCAGAGATGCATTTTGAGTCGCTGATTGAATTTGATGCTCAAGATCTGGAATCCTTTCCGTAGAATTCGCCTATTCTCTGTGATCGAATGGACCCTGTTTCCCCTCTTTCCATGATTTGATGAGTTGTAGGCCTTAATTGTGAGATGAAAATATGGATAAAATGTCGTTGGGAAATTATAAGAACAAAAATTTCAAAAAATAAATATAAATTAAGGCTCAGTTTCGGAACATCGATGTCATATAAAATATCTAGGTCTTGATTTTATAGAAATTTCTGTAAAAATTTTGAAAAAATTTATAGAAATGGATGGAAATTGTTATAATTAGTTAATGGAACTTTGATTGTGGTTAAATTAGACTATAATTAACTATTTTTAATCATTATTTCTATAAAGTAAGACTACATTTAGATTTATATTGTATCATTTCTATAGTTGAGCTAGCATAGCTAATTGCTACGAAAAACTTGAAATTTTGAAATATCTATGGAAATATCGAAATATCAATGAAAATTTCAAAATTTTGAGAAATTGGAGGAAAATCGAAATTTCGAGAAATTGAGGGAAAATTGAAATTCCCCCTTACCGAAATTTTGAACCTATTAAAATCTTGAAATTTTGAGATTTCAATCGAAATTTCAAACTATGACTAGGGTTAAGGTTGTTATCAAATGTATGAAAATGAGCCAATTTATTTACAAATATAACAAAATATTACTGTTTCTAATAGACATCTATCCGTGTCTATCAGTTATCACTTATACTTATAGATATCCATGAGCGAGTAATAGACTGACATTTTATTATATTTGAAAATAATTTCAGCAGTTTTGTCATTTAAAACAATAATCTAATAAACTAGTAAATAAATATTTTATGATTTTAAAATAAGTTATAACATGTTTATTATTTATATTATAAAAAATTATTTTAATGACAAAACTGCTGAAAATATTTATGAAATAATAACAAAATTATGAACTGTATCTTTTATGACATAGATAGATACAAATAATAGTCTATCACGGTATATCGCAAATAGACAGTAAAATTTTGTTATATTTATAAATATTTTGATTCATTTTTATATATTTAAAAATCGTCTTTCTCAAAAATCATTTAATACTGGATGGTTGTGTGTGTGTGGTGCAGTACTATTGGTGAGATGCTGGGCAAGGCCTTGGATTTCACTTTTCTTGATGCCGATGATTTTCATCCAATCTCTAACAAGGGTACCACAAGCTCATTTCTTCACTTGTTTTATTTTGGTTTTGGTCCTTCAACTTATTTCTTTTTTCTTATACTTTCCTAAATCATTTTCATTCTTAGAACTAACATTTAGGAACGTTTGAGGTAAAGAGTTAGTTATTATAATCCTATATTATTATAGTTAGATTATAATAGTTTGTGTTTGAGATGTAGATTATTTTAGTTTGGATTATAATAGTATGTATTTGAGATGTAAACTATAATTAAAAAATAATTAAAAAATGATGAATGTAACTGTAACAAATAGTAAAAACGGTAACAAATGAGTGTTGATGGAGTATACAAAATAATATGTACTATAACAAGAAATGATTATTATAGTCTTAGAATAGTCATTGCATTAAACACATCCTTAAAGTTTAAATTATAATTTTGGTTCATACGAATAAAAAAATATATTAGAATTTAATCTCTATAGTTTTTAAAGTTAGAATTTAGTTTTTACGGTATGATAAAGAGATTAAACTAGAATTTAATTTTTATGATTTGATAAAAAAAATTTCCTAAATAAATAATTCTTATGATCTAATAAACCTCTTGTGTATGGACTATTAAGAGGAGTGAGGATTAAATTTTAATTATAAATTAGAGAGACTTAATTCTAGTTTTCTCCAAATCATATGGATATGGACTACATTTGTAATTTAACCTTTTATTTATTTAAAATTGGACAAAAACATGATTTTTTTTATTCATTTACTTAGATGATTAAGTATACAAAATCTTGACAATGTTGAGCGTTCAATTGATATAAAACATGTAATCTCGCTAAGAGAGGTTTAACTACACTTTTAGTTTTGAAATTTGTTCAATATTTGTTAAATTGTTATGGAAAATTTCAAGCAAAACTTTAAAAACTAAACTCAAAAAAGTTATCAAATGAGACTTAAAAGAGTATTATTTATTTGTTTTTTTCTAATTTTTTAGTCAACTTAAATATTAGTTAAGATATTGTTGACATTTTTCAATTTAGCTATTCATTGTTTAATGAATGTTTAGACTGATGATAAACTTACCTTTTATAAATTGTAGGTTAAATTACAAATTTAGTCTCCAAACTTTTGATATTTTTTTAAAAAATTCATAGATATGTACAAAAAAAAAAAAAAAAAGAATTTAATGTTTATACTTCTTTTAGATATAAAATTCAAATTTATAATCTAACAAATCCTAATTGAAAGTTAAAGGATCTATTAACACAAAATTAAAAGTTTAGGAAGTTATTAAATACTTTTGAAAGTCAACGTCTAATGATCAAATAAACATAAATCTCAAAATTCAATAGCTAAACTTGTAATTTAACTTAAATTCTATTTATTTATCCAAATTTTAAGAAAGCGGTAGACATTCTGAGTAATTCATTAAATTTTGTTCAGTTTACTAAGCATAAGTGTTCATCATCAATCAATTTTAGCATTCACAATATGGACTAAATTTGAAAATTTTAATTAGTAAAGTACTAAACTGAAACTTTGTAAAACAGAAGTAAATTGAAAAAGATGCTTAAACATTATGAACAAATAAAAGTAATTTTCAAAACTTCTATAGATTTTGGCTTCGAGTATTTTTTTTTTTTCGCAAGTGTTCTAATTGAAAAACAACTCTTCAAAAGTCATTTCAAACTCACCCTGAGATTGAAGCTAACATTTGTATATCACCATTATGAGCAGAAAAAATGTCGAAAGGAATCCCTCTCTCGGACGAAGATCGGATACCATGGCTCGAGAAGATTCGAGATACCTTGAGAGAAAACATAACATGTAGAAGAAGTGTAGTTCTTGGTTGTTCAGCTCTACAAAAACACTATAGAGAGATTCTGAGATCAAGTGATCAAAATTATGAAAAAATGGAAACAATGAAATGTGTGGTGAAGTTTGTTCTATTGGATGCTCCAGCTGAAGTGATTGCTTCAAGATTAGAGAAAAGAGCCAAAGAAGGGAACCATTTCATGCCTTCAACTCTTTTGAAATCCCAATTGGATTTGTTGCAAATTGATGATAGTGAAGGTATTATAAAAGTTGATGCTACTCTTACTCCACAAGCAATTGTTAGTAATATTATTATGTTGGTTTTTGGATCTTTGGTTTTAACTAACAATGAGAAATCATAGTTTTGATTTTCTCATCCTCTTCCTTCTTACAAATTTCTTGTTTCTTGGAAAAAGGAAACAAAAATGTGATTCGCAATTGAAAACTGAGATATTTAAAGGATGTTTGAAGTGCAAAGAGAGTTGTGAGGTTATATAATCTACGGAGTTGAATAGTTTGTGTTTGAGGTACAGTATACTTTAATATGGATTCAAGAAGCCTAGAATTTTTTTAATGCATTTAGGAAATATATTTTTGAAGTGCTAGTTTA

mRNA sequence

GGAAAAATAAGGGCCAAATTGGTAATTTTAACAATAGATATTAATTTTGTTCTCGTCTTCAATTCTCAACTGCCATCTCTCACCACTTTGCCCAAAAATTTCATCATTCTCTGTCCATCACCTGGCATAAGGATTCTAGCATCCAAATTTGAATGCTATGGCGATCGTGCTCATGGGCGTCTGCGGTTCTGGAAAATCTACTATTGGTGAGATGCTGGGCAAGGCCTTGGATTTCACTTTTCTTGATGCCGATGATTTTCATCCAATCTCTAACAAGGAAAAAATGTCGAAAGGAATCCCTCTCTCGGACGAAGATCGGATACCATGGCTCGAGAAGATTCGAGATACCTTGAGAGAAAACATAACATGTAGAAGAAGTGTAGTTCTTGGTTGTTCAGCTCTACAAAAACACTATAGAGAGATTCTGAGATCAAGTGATCAAAATTATGAAAAAATGGAAACAATGAAATGTGTGGTGAAGTTTGTTCTATTGGATGCTCCAGCTGAAGTGATTGCTTCAAGATTAGAGAAAAGAGCCAAAGAAGGGAACCATTTCATGCCTTCAACTCTTTTGAAATCCCAATTGGATTTGTTGCAAATTGATGATAGTGAAGGTATTATAAAAGTTGATGCTACTCTTACTCCACAAGCAATTGTTAGTAATATTATTATGTTGGTTTTTGGATCTTTGGTTTTAACTAACAATGAGAAATCATAGTTTTGATTTTCTCATCCTCTTCCTTCTTACAAATTTCTTGTTTCTTGGAAAAAGGAAACAAAAATGTGATTCGCAATTGAAAACTGAGATATTTAAAGGATGTTTGAAGTGCAAAGAGAGTTGTGAGGTTATATAATCTACGGAGTTGAATAGTTTGTGTTTGAGGTACAGTATACTTTAATATGGATTCAAGAAGCCTAGAATTTTTTTAATGCATTTAGGAAATATATTTTTGAAGTGCTAGTTTA

Coding sequence (CDS)

ATGGCGATCGTGCTCATGGGCGTCTGCGGTTCTGGAAAATCTACTATTGGTGAGATGCTGGGCAAGGCCTTGGATTTCACTTTTCTTGATGCCGATGATTTTCATCCAATCTCTAACAAGGAAAAAATGTCGAAAGGAATCCCTCTCTCGGACGAAGATCGGATACCATGGCTCGAGAAGATTCGAGATACCTTGAGAGAAAACATAACATGTAGAAGAAGTGTAGTTCTTGGTTGTTCAGCTCTACAAAAACACTATAGAGAGATTCTGAGATCAAGTGATCAAAATTATGAAAAAATGGAAACAATGAAATGTGTGGTGAAGTTTGTTCTATTGGATGCTCCAGCTGAAGTGATTGCTTCAAGATTAGAGAAAAGAGCCAAAGAAGGGAACCATTTCATGCCTTCAACTCTTTTGAAATCCCAATTGGATTTGTTGCAAATTGATGATAGTGAAGGTATTATAAAAGTTGATGCTACTCTTACTCCACAAGCAATTGTTAGTAATATTATTATGTTGGTTTTTGGATCTTTGGTTTTAACTAACAATGAGAAATCATAG

Protein sequence

MAIVLMGVCGSGKSTIGEMLGKALDFTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEKIRDTLRENITCRRSVVLGCSALQKHYREILRSSDQNYEKMETMKCVVKFVLLDAPAEVIASRLEKRAKEGNHFMPSTLLKSQLDLLQIDDSEGIIKVDATLTPQAIVSNIIMLVFGSLVLTNNEKS
Homology
BLAST of Bhi12G000503 vs. TAIR 10
Match: AT2G16790.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 205.3 bits (521), Expect = 4.3e-53
Identity = 103/176 (58.52%), Postives = 132/176 (75.00%), Query Frame = 0

Query: 3   IVLMGVCGSGKSTIGEMLGKALDFTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEKIR 62
           I +MGV G+GKSTIG+MLGKAL   FLDADDFH +SN++KM +GI LSDEDR+PWLEKI+
Sbjct: 12  IAIMGVSGAGKSTIGKMLGKALSCDFLDADDFHSLSNRDKMRQGIALSDEDRMPWLEKIQ 71

Query: 63  DTLRENITCRRSVVLGCSALQKHYREILRSSDQNYEKMETMKCVVKFVLLDAPAEVIASR 122
           ++LR+ +    +VVL CS+L+K YREILR SD +Y+      C V FVLL+  AEVIA+R
Sbjct: 72  ESLRKRLLDGETVVLACSSLRKQYREILRGSDPDYKPGSYTSCKVTFVLLEGNAEVIAAR 131

Query: 123 LEKRAKEGNHFMPSTLLKSQLDLLQIDDSEGIIKVDATLTPQAIVSNIIMLVFGSL 179
           L+KRA E  HFMP TLL+SQ DLLQ D+ E I K+   L+P+ IV+ I+ +V  SL
Sbjct: 132 LQKRASEEEHFMPLTLLQSQFDLLQADECEKIFKISVVLSPEVIVNTILEMVANSL 187

BLAST of Bhi12G000503 vs. TAIR 10
Match: AT2G16790.2 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 205.3 bits (521), Expect = 4.3e-53
Identity = 103/176 (58.52%), Postives = 132/176 (75.00%), Query Frame = 0

Query: 3   IVLMGVCGSGKSTIGEMLGKALDFTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEKIR 62
           I +MGV G+GKSTIG+MLGKAL   FLDADDFH +SN++KM +GI LSDEDR+PWLEKI+
Sbjct: 10  IAIMGVSGAGKSTIGKMLGKALSCDFLDADDFHSLSNRDKMRQGIALSDEDRMPWLEKIQ 69

Query: 63  DTLRENITCRRSVVLGCSALQKHYREILRSSDQNYEKMETMKCVVKFVLLDAPAEVIASR 122
           ++LR+ +    +VVL CS+L+K YREILR SD +Y+      C V FVLL+  AEVIA+R
Sbjct: 70  ESLRKRLLDGETVVLACSSLRKQYREILRGSDPDYKPGSYTSCKVTFVLLEGNAEVIAAR 129

Query: 123 LEKRAKEGNHFMPSTLLKSQLDLLQIDDSEGIIKVDATLTPQAIVSNIIMLVFGSL 179
           L+KRA E  HFMP TLL+SQ DLLQ D+ E I K+   L+P+ IV+ I+ +V  SL
Sbjct: 130 LQKRASEEEHFMPLTLLQSQFDLLQADECEKIFKISVVLSPEVIVNTILEMVANSL 185

BLAST of Bhi12G000503 vs. ExPASy Swiss-Prot
Match: Q9SLE0 (Gluconokinase OS=Arabidopsis thaliana OX=3702 GN=At2g16790 PE=2 SV=2)

HSP 1 Score: 205.3 bits (521), Expect = 6.0e-52
Identity = 103/176 (58.52%), Postives = 132/176 (75.00%), Query Frame = 0

Query: 3   IVLMGVCGSGKSTIGEMLGKALDFTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEKIR 62
           I +MGV G+GKSTIG+MLGKAL   FLDADDFH +SN++KM +GI LSDEDR+PWLEKI+
Sbjct: 12  IAIMGVSGAGKSTIGKMLGKALSCDFLDADDFHSLSNRDKMRQGIALSDEDRMPWLEKIQ 71

Query: 63  DTLRENITCRRSVVLGCSALQKHYREILRSSDQNYEKMETMKCVVKFVLLDAPAEVIASR 122
           ++LR+ +    +VVL CS+L+K YREILR SD +Y+      C V FVLL+  AEVIA+R
Sbjct: 72  ESLRKRLLDGETVVLACSSLRKQYREILRGSDPDYKPGSYTSCKVTFVLLEGNAEVIAAR 131

Query: 123 LEKRAKEGNHFMPSTLLKSQLDLLQIDDSEGIIKVDATLTPQAIVSNIIMLVFGSL 179
           L+KRA E  HFMP TLL+SQ DLLQ D+ E I K+   L+P+ IV+ I+ +V  SL
Sbjct: 132 LQKRASEEEHFMPLTLLQSQFDLLQADECEKIFKISVVLSPEVIVNTILEMVANSL 187

BLAST of Bhi12G000503 vs. ExPASy Swiss-Prot
Match: B0BML1 (Probable gluconokinase OS=Xenopus tropicalis OX=8364 GN=idnk PE=2 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 1.9e-29
Identity = 73/176 (41.48%), Postives = 103/176 (58.52%), Query Frame = 0

Query: 1   MAIVLMGVCGSGKSTIGEMLGKALDFTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEK 60
           M IV+MGV GSGK+ +G  L K L + F DADD+HP+ NKEKMS+G PL+D+DR PWL +
Sbjct: 1   MIIVIMGVSGSGKTVVGSQLAKKLGWNFYDADDYHPLENKEKMSQGTPLNDQDRHPWLCE 60

Query: 61  IRDTLRENITCRRSVVLGCSALQKHYREILRSSD-----QNYEKMETMKCVVKFVLLDAP 120
           + + +       + VVL CSAL++ YR  L +       +NY++ + +     FV L   
Sbjct: 61  LHEIMMREKALGQHVVLACSALKRAYRSTLLTGSTPHWPENYQENDDLSSDTLFVHLHGS 120

Query: 121 AEVIASRLEKRAKEGNHFMPSTLLKSQLDLLQIDDS-EGIIKVDATLTPQAIVSNI 171
            E+++ RL +R     HFMP TLL SQ+D L+   + E  I +D       IVS I
Sbjct: 121 LEILSRRLLERK---GHFMPRTLLDSQIDTLEPPSAPERFIAIDVDKDISVIVSEI 173

BLAST of Bhi12G000503 vs. ExPASy Swiss-Prot
Match: Q5T6J7 (Probable gluconokinase OS=Homo sapiens OX=9606 GN=IDNK PE=1 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 7.9e-28
Identity = 73/179 (40.78%), Postives = 107/179 (59.78%), Query Frame = 0

Query: 2   AIVLMGVCGSGKSTIGEMLGKALDFTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEKI 61
           A+++MGV GSGKST+G +L   L + F DADD+HP  N+ KM KGIPL+D+DRIPWL  +
Sbjct: 6   ALLVMGVSGSGKSTVGALLASELGWKFYDADDYHPEENRRKMGKGIPLNDQDRIPWLCNL 65

Query: 62  RDTLRENITCRRSVVLGCSALQKHYREILRSS--------DQNYEKMETMKCVVKFVLLD 121
            D L  ++   + VVL CSAL+K YR+IL           +++ ++ +  +  +  V L 
Sbjct: 66  HDILLRDVASGQRVVLACSALKKTYRDILTQGKDGVALKCEESGKEAKQAEMQLLVVHLS 125

Query: 122 APAEVIASRLEKRAKEGNHFMPSTLLKSQLDLLQIDDS-EGIIKVDATLTPQAIVSNII 172
              EVI+ RL KR  EG HFMP  LL+SQ + L+   + E  I++        I++ I+
Sbjct: 126 GSFEVISGRLLKR--EG-HFMPPELLQSQFETLEPPAAPENFIQISVDKNVSEIIATIM 181

BLAST of Bhi12G000503 vs. ExPASy Swiss-Prot
Match: P39208 (Thermosensitive gluconokinase OS=Escherichia coli (strain K12) OX=83333 GN=idnK PE=3 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 6.7e-27
Identity = 66/159 (41.51%), Postives = 93/159 (58.49%), Query Frame = 0

Query: 2   AIVLMGVCGSGKSTIGEMLGKALDFTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEKI 61
           + +LMGV GSGK+ IG  +   L   F+D DD HP  N +KMS+GIPLSDEDR+PWLE++
Sbjct: 5   SFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNIDKMSQGIPLSDEDRLPWLERL 64

Query: 62  RDTLRENITCRRSVVLGCSALQKHYREILRSSDQNYEKMETMKCVVKFVLLDAPAEVIAS 121
            D          +  + CS+L+K YR+ILR    +          V F+ LD   E I +
Sbjct: 65  NDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPH----------VHFLWLDGDYETILA 124

Query: 122 RLEKRAKEGNHFMPSTLLKSQLDLLQID--DSEGIIKVD 159
           R+++RA    HFMP  LLKSQ + L+    D + I+++D
Sbjct: 125 RMQRRA---GHFMPVALLKSQFEALERPQADEQDIVRID 150

BLAST of Bhi12G000503 vs. ExPASy Swiss-Prot
Match: Q8R0J8 (Probable gluconokinase OS=Mus musculus OX=10090 GN=Idnk PE=1 SV=2)

HSP 1 Score: 117.5 bits (293), Expect = 1.6e-25
Identity = 67/154 (43.51%), Postives = 93/154 (60.39%), Query Frame = 0

Query: 3   IVLMGVCGSGKSTIGEMLGKALDFTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEKIR 62
           +++MGV GSGKST+G +L   L + F DADD+H   N+ KM+KG+PLSD+DRIPWL  + 
Sbjct: 7   LLVMGVSGSGKSTVGALLASKLGWKFYDADDYHSEENRIKMAKGVPLSDQDRIPWLCTLH 66

Query: 63  DTLRENITCRRSVVLGCSALQKHYREI---------LRSSDQNYEKMETMKCVVKFVLLD 122
           D L  ++   + VVL CSAL+K YR+I         L+S D   E +   K +V  V L 
Sbjct: 67  DILLRDVALGQPVVLACSALKKTYRDILIRGGSDAPLKSDDSAKEPLAGGKLLV--VYLC 126

Query: 123 APAEVIASRLEKRAKEGNHFMPSTLLKSQLDLLQ 148
              ++I  RL +R     HFMP  LL+SQ  +L+
Sbjct: 127 GSFDIIYGRLLQRK---GHFMPPELLQSQFSILE 155

BLAST of Bhi12G000503 vs. NCBI nr
Match: XP_038874456.1 (gluconokinase [Benincasa hispida] >XP_038874457.1 gluconokinase [Benincasa hispida])

HSP 1 Score: 365.5 bits (937), Expect = 2.6e-97
Identity = 186/186 (100.00%), Postives = 186/186 (100.00%), Query Frame = 0

Query: 1   MAIVLMGVCGSGKSTIGEMLGKALDFTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEK 60
           MAIVLMGVCGSGKSTIGEMLGKALDFTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEK
Sbjct: 1   MAIVLMGVCGSGKSTIGEMLGKALDFTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEK 60

Query: 61  IRDTLRENITCRRSVVLGCSALQKHYREILRSSDQNYEKMETMKCVVKFVLLDAPAEVIA 120
           IRDTLRENITCRRSVVLGCSALQKHYREILRSSDQNYEKMETMKCVVKFVLLDAPAEVIA
Sbjct: 61  IRDTLRENITCRRSVVLGCSALQKHYREILRSSDQNYEKMETMKCVVKFVLLDAPAEVIA 120

Query: 121 SRLEKRAKEGNHFMPSTLLKSQLDLLQIDDSEGIIKVDATLTPQAIVSNIIMLVFGSLVL 180
           SRLEKRAKEGNHFMPSTLLKSQLDLLQIDDSEGIIKVDATLTPQAIVSNIIMLVFGSLVL
Sbjct: 121 SRLEKRAKEGNHFMPSTLLKSQLDLLQIDDSEGIIKVDATLTPQAIVSNIIMLVFGSLVL 180

Query: 181 TNNEKS 187
           TNNEKS
Sbjct: 181 TNNEKS 186

BLAST of Bhi12G000503 vs. NCBI nr
Match: KAA0055116.1 (thermosensitive gluconokinase [Cucumis melo var. makuwa] >TYK20744.1 thermosensitive gluconokinase [Cucumis melo var. makuwa])

HSP 1 Score: 307.8 bits (787), Expect = 6.5e-80
Identity = 158/175 (90.29%), Postives = 164/175 (93.71%), Query Frame = 0

Query: 1   MAIVLMGVCGSGKSTIGEMLGKALDFTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEK 60
           M IVLMGVCGSGKSTIGEMLGKALDFTFLDAD FHPISNKEKMSKGIPLSDEDR+PWLEK
Sbjct: 1   MVIVLMGVCGSGKSTIGEMLGKALDFTFLDADHFHPISNKEKMSKGIPLSDEDRMPWLEK 60

Query: 61  IRDTLRENITCRRSVVLGCSALQKHYREILRSSDQNYEKME-TMKCVVKFVLLDAPAEVI 120
           IRDTLRENI  +RSVV+GCSALQKHYREILRSSD NYEKM  TMKCVVKFVLLDAPAEVI
Sbjct: 61  IRDTLRENIASKRSVVVGCSALQKHYREILRSSDPNYEKMGITMKCVVKFVLLDAPAEVI 120

Query: 121 ASRLEKRAKEGNHFMPSTLLKSQLDLLQIDDSEGIIKVDATLTPQAIVSNIIMLV 175
           A RLEKRAKEGNHFMPS LLKSQLDLLQIDDSEGI+KVDAT TPQAI+SNI+ LV
Sbjct: 121 ALRLEKRAKEGNHFMPSALLKSQLDLLQIDDSEGIVKVDATQTPQAILSNILNLV 175

BLAST of Bhi12G000503 vs. NCBI nr
Match: XP_008467300.1 (PREDICTED: thermosensitive gluconokinase [Cucumis melo])

HSP 1 Score: 307.8 bits (787), Expect = 6.5e-80
Identity = 158/175 (90.29%), Postives = 164/175 (93.71%), Query Frame = 0

Query: 1   MAIVLMGVCGSGKSTIGEMLGKALDFTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEK 60
           M IVLMGVCGSGKSTIGEMLGKALDFTFLDAD FHPISNKEKMSKGIPLSDEDR+PWLEK
Sbjct: 6   MVIVLMGVCGSGKSTIGEMLGKALDFTFLDADHFHPISNKEKMSKGIPLSDEDRMPWLEK 65

Query: 61  IRDTLRENITCRRSVVLGCSALQKHYREILRSSDQNYEKME-TMKCVVKFVLLDAPAEVI 120
           IRDTLRENI  +RSVV+GCSALQKHYREILRSSD NYEKM  TMKCVVKFVLLDAPAEVI
Sbjct: 66  IRDTLRENIASKRSVVVGCSALQKHYREILRSSDPNYEKMGITMKCVVKFVLLDAPAEVI 125

Query: 121 ASRLEKRAKEGNHFMPSTLLKSQLDLLQIDDSEGIIKVDATLTPQAIVSNIIMLV 175
           A RLEKRAKEGNHFMPS LLKSQLDLLQIDDSEGI+KVDAT TPQAI+SNI+ LV
Sbjct: 126 ALRLEKRAKEGNHFMPSALLKSQLDLLQIDDSEGIVKVDATQTPQAILSNILNLV 180

BLAST of Bhi12G000503 vs. NCBI nr
Match: XP_004143662.1 (gluconokinase isoform X1 [Cucumis sativus])

HSP 1 Score: 305.8 bits (782), Expect = 2.5e-79
Identity = 155/175 (88.57%), Postives = 164/175 (93.71%), Query Frame = 0

Query: 1   MAIVLMGVCGSGKSTIGEMLGKALDFTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEK 60
           M I+LMGVCGSGKSTIGEMLGKALDFTFLDAD FHPISNKEKMSKGIPLSDEDR+PWLEK
Sbjct: 6   MVIILMGVCGSGKSTIGEMLGKALDFTFLDADHFHPISNKEKMSKGIPLSDEDRMPWLEK 65

Query: 61  IRDTLRENITCRRSVVLGCSALQKHYREILRSSDQNYEKME-TMKCVVKFVLLDAPAEVI 120
           IRDTLRENI C+ SVVLGCSALQKHYREILRSSD NYEK+  TMKCVVKFVLLDAPAEVI
Sbjct: 66  IRDTLRENIACKSSVVLGCSALQKHYREILRSSDPNYEKVGITMKCVVKFVLLDAPAEVI 125

Query: 121 ASRLEKRAKEGNHFMPSTLLKSQLDLLQIDDSEGIIKVDATLTPQAIVSNIIMLV 175
           A RLEKRAKEGNHFMPS LLKSQLDLLQI+D+EGI+KVDAT TPQAI+SNI+ LV
Sbjct: 126 ALRLEKRAKEGNHFMPSALLKSQLDLLQIEDTEGIVKVDATQTPQAILSNILNLV 180

BLAST of Bhi12G000503 vs. NCBI nr
Match: KAE8647942.1 (hypothetical protein Csa_000183 [Cucumis sativus])

HSP 1 Score: 298.1 bits (762), Expect = 5.2e-77
Identity = 150/168 (89.29%), Postives = 158/168 (94.05%), Query Frame = 0

Query: 1   MAIVLMGVCGSGKSTIGEMLGKALDFTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEK 60
           M I+LMGVCGSGKSTIGEMLGKALDFTFLDAD FHPISNKEKMSKGIPLSDEDR+PWLEK
Sbjct: 6   MVIILMGVCGSGKSTIGEMLGKALDFTFLDADHFHPISNKEKMSKGIPLSDEDRMPWLEK 65

Query: 61  IRDTLRENITCRRSVVLGCSALQKHYREILRSSDQNYEKME-TMKCVVKFVLLDAPAEVI 120
           IRDTLRENI C+ SVVLGCSALQKHYREILRSSD NYEK+  TMKCVVKFVLLDAPAEVI
Sbjct: 66  IRDTLRENIACKSSVVLGCSALQKHYREILRSSDPNYEKVGITMKCVVKFVLLDAPAEVI 125

Query: 121 ASRLEKRAKEGNHFMPSTLLKSQLDLLQIDDSEGIIKVDATLTPQAIV 168
           A RLEKRAKEGNHFMPS LLKSQLDLLQI+D+EGI+KVDAT TPQAI+
Sbjct: 126 ALRLEKRAKEGNHFMPSALLKSQLDLLQIEDTEGIVKVDATQTPQAIL 173

BLAST of Bhi12G000503 vs. ExPASy TrEMBL
Match: A0A5A7ULE7 (Gluconokinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold480G001470 PE=3 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 3.2e-80
Identity = 158/175 (90.29%), Postives = 164/175 (93.71%), Query Frame = 0

Query: 1   MAIVLMGVCGSGKSTIGEMLGKALDFTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEK 60
           M IVLMGVCGSGKSTIGEMLGKALDFTFLDAD FHPISNKEKMSKGIPLSDEDR+PWLEK
Sbjct: 1   MVIVLMGVCGSGKSTIGEMLGKALDFTFLDADHFHPISNKEKMSKGIPLSDEDRMPWLEK 60

Query: 61  IRDTLRENITCRRSVVLGCSALQKHYREILRSSDQNYEKME-TMKCVVKFVLLDAPAEVI 120
           IRDTLRENI  +RSVV+GCSALQKHYREILRSSD NYEKM  TMKCVVKFVLLDAPAEVI
Sbjct: 61  IRDTLRENIASKRSVVVGCSALQKHYREILRSSDPNYEKMGITMKCVVKFVLLDAPAEVI 120

Query: 121 ASRLEKRAKEGNHFMPSTLLKSQLDLLQIDDSEGIIKVDATLTPQAIVSNIIMLV 175
           A RLEKRAKEGNHFMPS LLKSQLDLLQIDDSEGI+KVDAT TPQAI+SNI+ LV
Sbjct: 121 ALRLEKRAKEGNHFMPSALLKSQLDLLQIDDSEGIVKVDATQTPQAILSNILNLV 175

BLAST of Bhi12G000503 vs. ExPASy TrEMBL
Match: A0A1S3CTF0 (Gluconokinase OS=Cucumis melo OX=3656 GN=LOC103504681 PE=3 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 3.2e-80
Identity = 158/175 (90.29%), Postives = 164/175 (93.71%), Query Frame = 0

Query: 1   MAIVLMGVCGSGKSTIGEMLGKALDFTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEK 60
           M IVLMGVCGSGKSTIGEMLGKALDFTFLDAD FHPISNKEKMSKGIPLSDEDR+PWLEK
Sbjct: 6   MVIVLMGVCGSGKSTIGEMLGKALDFTFLDADHFHPISNKEKMSKGIPLSDEDRMPWLEK 65

Query: 61  IRDTLRENITCRRSVVLGCSALQKHYREILRSSDQNYEKME-TMKCVVKFVLLDAPAEVI 120
           IRDTLRENI  +RSVV+GCSALQKHYREILRSSD NYEKM  TMKCVVKFVLLDAPAEVI
Sbjct: 66  IRDTLRENIASKRSVVVGCSALQKHYREILRSSDPNYEKMGITMKCVVKFVLLDAPAEVI 125

Query: 121 ASRLEKRAKEGNHFMPSTLLKSQLDLLQIDDSEGIIKVDATLTPQAIVSNIIMLV 175
           A RLEKRAKEGNHFMPS LLKSQLDLLQIDDSEGI+KVDAT TPQAI+SNI+ LV
Sbjct: 126 ALRLEKRAKEGNHFMPSALLKSQLDLLQIDDSEGIVKVDATQTPQAILSNILNLV 180

BLAST of Bhi12G000503 vs. ExPASy TrEMBL
Match: A0A0A0KN86 (Gluconokinase OS=Cucumis sativus OX=3659 GN=Csa_5G169050 PE=3 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 1.2e-79
Identity = 155/175 (88.57%), Postives = 164/175 (93.71%), Query Frame = 0

Query: 1   MAIVLMGVCGSGKSTIGEMLGKALDFTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEK 60
           M I+LMGVCGSGKSTIGEMLGKALDFTFLDAD FHPISNKEKMSKGIPLSDEDR+PWLEK
Sbjct: 6   MVIILMGVCGSGKSTIGEMLGKALDFTFLDADHFHPISNKEKMSKGIPLSDEDRMPWLEK 65

Query: 61  IRDTLRENITCRRSVVLGCSALQKHYREILRSSDQNYEKME-TMKCVVKFVLLDAPAEVI 120
           IRDTLRENI C+ SVVLGCSALQKHYREILRSSD NYEK+  TMKCVVKFVLLDAPAEVI
Sbjct: 66  IRDTLRENIACKSSVVLGCSALQKHYREILRSSDPNYEKVGITMKCVVKFVLLDAPAEVI 125

Query: 121 ASRLEKRAKEGNHFMPSTLLKSQLDLLQIDDSEGIIKVDATLTPQAIVSNIIMLV 175
           A RLEKRAKEGNHFMPS LLKSQLDLLQI+D+EGI+KVDAT TPQAI+SNI+ LV
Sbjct: 126 ALRLEKRAKEGNHFMPSALLKSQLDLLQIEDTEGIVKVDATQTPQAILSNILNLV 180

BLAST of Bhi12G000503 vs. ExPASy TrEMBL
Match: A0A6J1E468 (Gluconokinase OS=Momordica charantia OX=3673 GN=LOC111025918 PE=3 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 1.6e-71
Identity = 140/176 (79.55%), Postives = 157/176 (89.20%), Query Frame = 0

Query: 3   IVLMGVCGSGKSTIGEMLGKALDFTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEKIR 62
           IVLMGVCGSGKSTIGEML KA++  FLDADDFHP SNKEKMSKGIPLS+EDR+PWLEK+R
Sbjct: 14  IVLMGVCGSGKSTIGEMLAKAMNLNFLDADDFHPNSNKEKMSKGIPLSNEDRLPWLEKVR 73

Query: 63  DTLRENITCRRSVVLGCSALQKHYREILRSSDQNYEKMETMKCVVKFVLLDAPAEVIASR 122
           D L+E+I C++SVVLGCSALQK YREILRS+D NY   E   CVVKFVLLDAP EVIA R
Sbjct: 74  DALKESIACKKSVVLGCSALQKQYREILRSADPNY---EIGSCVVKFVLLDAPPEVIAYR 133

Query: 123 LEKRAKEGNHFMPSTLLKSQLDLLQIDDSEGIIKVDATLTPQAIVSNIIMLVFGSL 179
           LE+RAKEGNHFM STLL+SQLDLL+IDDSEGI+KVDATLTPQ IV+NI+ +VFGSL
Sbjct: 134 LERRAKEGNHFMSSTLLESQLDLLEIDDSEGILKVDATLTPQVIVTNILKVVFGSL 186

BLAST of Bhi12G000503 vs. ExPASy TrEMBL
Match: A0A6J1H380 (Gluconokinase OS=Cucurbita moschata OX=3662 GN=LOC111459704 PE=3 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 3.5e-71
Identity = 146/186 (78.49%), Postives = 160/186 (86.02%), Query Frame = 0

Query: 1   MAIVLMGVCGSGKSTIGEMLGKALDFTFLDADDFHPISNKEKMSKGIPLSDEDRIPWLEK 60
           MAIVLMGVCGSGKSTIG ML +A+  TFLDADDFHP SNKEKMSKGIPLSDEDRIPWLEK
Sbjct: 11  MAIVLMGVCGSGKSTIGAMLAEAMGLTFLDADDFHPSSNKEKMSKGIPLSDEDRIPWLEK 70

Query: 61  IRDTLRENITCRRSVVLGCSALQKHYREILRSSDQNYEKMETMKCVVKFVLLDAPAEVIA 120
           IRDTLRE +  + SVVLGCSALQK YR+ILRS+D NYE +  ++CVVKFVLLDAPAEVIA
Sbjct: 71  IRDTLREKMGSKNSVVLGCSALQKQYRDILRSADPNYEGI-GIRCVVKFVLLDAPAEVIA 130

Query: 121 SRLEKRAKEGNHFMPSTLLKSQLDLLQIDDSEGIIKVDATLTPQAIVSNIIMLVFGSLVL 180
            RLEKRAKEGNHFMPSTLL SQLDLLQID SEGI++VDAT +PQAIVS II LV G L L
Sbjct: 131 LRLEKRAKEGNHFMPSTLLNSQLDLLQIDVSEGILQVDATRSPQAIVSRIIKLVRGFLFL 190

Query: 181 TNNEKS 187
             + +S
Sbjct: 191 NIHNRS 195

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT2G16790.14.3e-5358.52P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT2G16790.24.3e-5358.52P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
Match NameE-valueIdentityDescription
Q9SLE06.0e-5258.52Gluconokinase OS=Arabidopsis thaliana OX=3702 GN=At2g16790 PE=2 SV=2[more]
B0BML11.9e-2941.48Probable gluconokinase OS=Xenopus tropicalis OX=8364 GN=idnk PE=2 SV=1[more]
Q5T6J77.9e-2840.78Probable gluconokinase OS=Homo sapiens OX=9606 GN=IDNK PE=1 SV=1[more]
P392086.7e-2741.51Thermosensitive gluconokinase OS=Escherichia coli (strain K12) OX=83333 GN=idnK ... [more]
Q8R0J81.6e-2543.51Probable gluconokinase OS=Mus musculus OX=10090 GN=Idnk PE=1 SV=2[more]
Match NameE-valueIdentityDescription
XP_038874456.12.6e-97100.00gluconokinase [Benincasa hispida] >XP_038874457.1 gluconokinase [Benincasa hispi... [more]
KAA0055116.16.5e-8090.29thermosensitive gluconokinase [Cucumis melo var. makuwa] >TYK20744.1 thermosensi... [more]
XP_008467300.16.5e-8090.29PREDICTED: thermosensitive gluconokinase [Cucumis melo][more]
XP_004143662.12.5e-7988.57gluconokinase isoform X1 [Cucumis sativus][more]
KAE8647942.15.2e-7789.29hypothetical protein Csa_000183 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A5A7ULE73.2e-8090.29Gluconokinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold480G001470... [more]
A0A1S3CTF03.2e-8090.29Gluconokinase OS=Cucumis melo OX=3656 GN=LOC103504681 PE=3 SV=1[more]
A0A0A0KN861.2e-7988.57Gluconokinase OS=Cucumis sativus OX=3659 GN=Csa_5G169050 PE=3 SV=1[more]
A0A6J1E4681.6e-7179.55Gluconokinase OS=Momordica charantia OX=3673 GN=LOC111025918 PE=3 SV=1[more]
A0A6J1H3803.5e-7178.49Gluconokinase OS=Cucurbita moschata OX=3662 GN=LOC111459704 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR01100SHIKIMTKNASEcoord: 107..124
score: 26.5
coord: 3..18
score: 51.92
IPR006001Carbohydrate kinase, thermoresistant glucokinaseTIGRFAMTIGR01313TIGR01313coord: 3..171
e-value: 3.3E-46
score: 155.0
IPR006001Carbohydrate kinase, thermoresistant glucokinasePANTHERPTHR43442GLUCONOKINASE-RELATEDcoord: 2..175
IPR006001Carbohydrate kinase, thermoresistant glucokinaseCDDcd02021GntKcoord: 3..159
e-value: 5.54898E-58
score: 177.059
IPR031322Shikimate kinase/gluconokinasePFAMPF01202SKIcoord: 10..144
e-value: 1.1E-12
score: 48.4
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 1..174
e-value: 4.8E-59
score: 201.0
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 1..175

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi12M000503Bhi12M000503mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046177 D-gluconate catabolic process
biological_process GO:0016310 phosphorylation
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0005524 ATP binding
molecular_function GO:0046316 gluconokinase activity
molecular_function GO:0016301 kinase activity