Bhi12G000502 (gene) Wax gourd (B227) v1

BLAST of Bhi12G000502 vs. TAIR 10
Match: AT3G03130.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G17160.1); Has 7130 Blast hits to 3690 proteins in 504 species: Archae - 22; Bacteria - 2666; Metazoa - 1654; Fungi - 721; Plants - 246; Viruses - 56; Other Eukaryotes - 1765 (source: NCBI BLink). )

HSP 1 Score: 55.5 bits (132), Expect = 2.2e-07
Identity = 165/601 (27.45%), Postives = 255/601 (42.43%), Query Frame = 0

Query: 1   MDFRTLSRKELQALCKRNKIPANITNVAMADALAALSSVEGIEEFFNGERSGVPESPMKP 60
           MDF +L R++LQ  CKRNKIPAN+TN+AMADAL  L  VEG++EF +      P     P
Sbjct: 1   MDFHSLPRRDLQFFCKRNKIPANMTNIAMADALRDLEIVEGMDEFMD------PSRDQSP 60

Query: 61  EVISSEIP---RTALRTSTRRKAVKDEA----ITTRTRRAAAARDTEE--SENRDLNVAL 120
             ++  +P   RTA RT TRRK+ KDE     + TR+    +     E   EN+D+N+ L
Sbjct: 61  TSVARNLPSAARTAART-TRRKSTKDETQSSELVTRSCYVVSKSLAGEMDQENKDMNM-L 120

Query: 121 TTPSLPGSRRRTAAASSACKKVDFQMTVDDQKEDNDLDKKKKAIEKTPAVPKSLRVAGAS 180
             PS+P SR           K+D     D   E N        + KTPA   + R   A+
Sbjct: 121 QNPSVPQSR---------AVKLDVN---DIMPEAN--------VSKTPAARSTRRAQAAA 180

Query: 181 TRKRTETRNNGAAEQRVYSTRRSVRFLEKNMESLSLGEDREMESITVHMSFDDMPNSSVG 240
           + K+ E      + QRVYSTRRSVR LE++M  LSL  +                     
Sbjct: 181 SSKKDE------SVQRVYSTRRSVRLLEESMADLSLKTN--------------------- 240

Query: 241 AGPVKEDTELETESK---KSDESESKLDKDTGVEIDDQEKNEMESEVELSEEEQEMVLDD 300
             PVK+  +    SK   KSDE+    DK  GV       + +E E + S+ + ++ +  
Sbjct: 241 -VPVKKHEDSPAGSKFQAKSDENSENTDKG-GVMSGRDLNDSLEKEWDGSKNDPDLDI-- 300

Query: 301 PLKSSEEKPATANNGDDTGTEAPHANSDVKCFSEDEEANEASKDDVSVEISAEEINDSDK 360
                       + GD T  +A          +  E  N      VS   S   +N+ + 
Sbjct: 301 ---------LYGDLGDITFFDAS---------TSKEHLNRTDSSTVSASDSFVLVNEHE- 360

Query: 361 LSQPTEDGDLTEVVDNSIIVEESGMEVESKQNESTCELKHIIEEVINEEVKLMNDTAE-- 420
               T   D   VVD++     +     +K++E   E   I  E  +EE +   D  E  
Sbjct: 361 ----TSQEDGFVVVDHATSTTTTNTLACNKESEP--EQMKIDSESESEETEYETDPWEGD 420

Query: 421 ----KLSLPHENVEEEAQVDDDVLSSDEESVMEVKDDQTQVEAENESTYELEHIIEKVNE 480
                +    E  E +    D+V   D  + + + D+  +++  + S   +E + E  +E
Sbjct: 421 DFGVAVHTNQEAFESKVSASDNVSKVDSVATVLIADESKELDF-SSSPLAVEELEEDSDE 480

Query: 481 ------EEVKLMKDT-AEELSLPHESVEEKTQVDDGVLSSDEESVIEVKDDQDEYDYESD 540
                  EV+L +++   E S+  ES E          SS   S+   +       +E++
Sbjct: 481 WSDYEIGEVELEENSCGSEESIEIESEEAPVSDKKTPASSSSSSLAGNETRTSLSPFEAE 515

Query: 541 PTIEEEASDADIEVD-----KKETEMEKVSVQAEEDDTTINTSMEEFPENVSEDLVDVKI 572
             +E E  D ++ V+     K E E++K   +   D+   + SM +  + V E  +  K 
Sbjct: 541 SILESE-EDKEMAVNNNGEGKAEAEVKKTKKKKTIDEELKDVSMRQLTKMVKELAIKSKQ 515

BLAST of Bhi12G000502 vs. TAIR 10
Match: AT5G17160.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G03130.1); Has 14330 Blast hits to 10381 proteins in 896 species: Archae - 94; Bacteria - 2881; Metazoa - 4019; Fungi - 1576; Plants - 515; Viruses - 110; Other Eukaryotes - 5135 (source: NCBI BLink). )

HSP 1 Score: 45.1 bits (105), Expect = 2.9e-04
Identity = 158/652 (24.23%), Postives = 273/652 (41.87%), Query Frame = 0

Query: 1   MDFRTLSRKELQALCKRNKIPANITNVAMADALAALSSVEGIEEFFNGERSGVPESPMKP 60
           MDF +L R++LQ LCKRNKIPAN+TN+AMADAL +L  V+G++E+ N   S  P SP   
Sbjct: 1   MDFHSLLRRDLQFLCKRNKIPANMTNIAMADALKSLEIVDGLDEYMNQSESSAPHSPTS- 60

Query: 61  EVISSEIPRTALRTSTRRKAVKDEAITTRTRRAAAARDTEESENRDLNVALTTPSLPGSR 120
             ++   P TA RT+ R+   K E   +    + + R T +S   D++            
Sbjct: 61  --VAKLPPSTATRTTRRKTTTKAEPQPSSQLVSRSCRSTSKSLAGDMD------------ 120

Query: 121 RRTAAASSACKKVDFQMTVDDQKEDNDLDKKKKAIEKTPAVPKSLRVAGASTRKRTETRN 180
                  +  K V  +M   + K + +       + KTPA   + + + A     T    
Sbjct: 121 -----QENINKNVAQEMKTSNVKFEAN-------VLKTPAAGSTRKTSAA-----TSCTK 180

Query: 181 NGAAEQRVYSTRRSVRFLEKNMESLSLGEDREMESITVHMSFDDMPNSSVGAGPVKEDTE 240
                Q VYSTRRS R LEK M  LSL   +  E++      D+ P  +       EDTE
Sbjct: 181 KDELVQSVYSTRRSTRLLEKCMADLSL---KTKETV------DNKPAKN-------EDTE 240

Query: 241 LETESKKSD----ESESKLDKDTGVEIDDQEKN-EMESEVELSEEEQEMVLDDPLKSSEE 300
            +  +++ +    E E    +D  V ++   +N + +S+    + E  +V+D   ++++E
Sbjct: 241 QKVSAQEKNLTGSEGEVIPGRDLSVSMEQVWENLKNDSDKIAGDLEVIVVMDANTEANKE 300

Query: 301 KPATANNGDDTGTEAPHANSDVKCFSEDEEANEASKDDVSVEISAEEINDSDKLSQPTED 360
           +       + T  +    NS V+   E+E      ++         + ND+D+     E 
Sbjct: 301 E-----MNEVTADKKESENSLVQVDKEEETLQAICEE-------GPKKNDNDQ-----EI 360

Query: 361 GDLTEVVDNSII--VEESGMEVESKQNES-----------TCELKHIIEEVINEEVKLMN 420
           GDL   VD S I  +E +  E  +  NES             E +H I+E   E    +N
Sbjct: 361 GDLVIYVDVSDIPLLESAITETHNDDNESKNVLAIDRSVDQQETEHAIQENDAEPETKVN 420

Query: 421 DTAEKL--SLPHENVEEEAQVDDDVLSSDEESVMEVKDDQTQVE-AENESTYELEHIIEK 480
            T      S   + ++E     + + + DEE++++  D  ++ E  EN S  + +  I +
Sbjct: 421 QTDSDAGDSKTKQAIQENDSEPEKINNFDEETMVDQTDSDSETEPEENHSGVDSDGTISE 480

Query: 481 VNEEEVKLMKDTA-EELSLPHESVEEKTQVDD-------GVLSSDEESVIEVKDDQDEYD 540
            +  +  +  D A EE++L        T  +         V+ +   S   V+    ++ 
Sbjct: 481 ADSNQAVVGSDIADEEMTLSGSEGSAATAPNSPPRLEEAKVIKTTLVSPFAVESISTQFP 540

Query: 541 YESDPTIEEEASDADIEVDKKETEMEKVSVQAEEDDTTINTSMEEFPENVSEDLVDVKIQ 600
             S  T   + S   +  + KE  ME + +    ++                       +
Sbjct: 541 RPSKSTTPLKNSPLKLVNENKENNMEMMMMNVNNNENG---------------------E 562

Query: 601 WQMEEEAPSKDIDVDAIDSQPKIPNDNV-QCEEALVEIIPTSSNNNGVQSLP 623
            + EE    K + +D    +  + N ++ Q E+ + E+   +SN   +Q LP
Sbjct: 601 SKGEEGKKKKKVTID----EENLKNTSIRQLEKMVKELSIKTSNRTALQVLP 562

BLAST of Bhi12G000502 vs. NCBI nr
Match: XP_038906922.1 (interaptin-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1338.6 bits (3463), Expect = 0.0e+00
Identity = 742/742 (100.00%), Postives = 742/742 (100.00%), Query Frame = 0

Query: 1   MDFRTLSRKELQALCKRNKIPANITNVAMADALAALSSVEGIEEFFNGERSGVPESPMKP 60
           MDFRTLSRKELQALCKRNKIPANITNVAMADALAALSSVEGIEEFFNGERSGVPESPMKP
Sbjct: 1   MDFRTLSRKELQALCKRNKIPANITNVAMADALAALSSVEGIEEFFNGERSGVPESPMKP 60

Query: 61  EVISSEIPRTALRTSTRRKAVKDEAITTRTRRAAAARDTEESENRDLNVALTTPSLPGSR 120
           EVISSEIPRTALRTSTRRKAVKDEAITTRTRRAAAARDTEESENRDLNVALTTPSLPGSR
Sbjct: 61  EVISSEIPRTALRTSTRRKAVKDEAITTRTRRAAAARDTEESENRDLNVALTTPSLPGSR 120

Query: 121 RRTAAASSACKKVDFQMTVDDQKEDNDLDKKKKAIEKTPAVPKSLRVAGASTRKRTETRN 180
           RRTAAASSACKKVDFQMTVDDQKEDNDLDKKKKAIEKTPAVPKSLRVAGASTRKRTETRN
Sbjct: 121 RRTAAASSACKKVDFQMTVDDQKEDNDLDKKKKAIEKTPAVPKSLRVAGASTRKRTETRN 180

Query: 181 NGAAEQRVYSTRRSVRFLEKNMESLSLGEDREMESITVHMSFDDMPNSSVGAGPVKEDTE 240
           NGAAEQRVYSTRRSVRFLEKNMESLSLGEDREMESITVHMSFDDMPNSSVGAGPVKEDTE
Sbjct: 181 NGAAEQRVYSTRRSVRFLEKNMESLSLGEDREMESITVHMSFDDMPNSSVGAGPVKEDTE 240

Query: 241 LETESKKSDESESKLDKDTGVEIDDQEKNEMESEVELSEEEQEMVLDDPLKSSEEKPATA 300
           LETESKKSDESESKLDKDTGVEIDDQEKNEMESEVELSEEEQEMVLDDPLKSSEEKPATA
Sbjct: 241 LETESKKSDESESKLDKDTGVEIDDQEKNEMESEVELSEEEQEMVLDDPLKSSEEKPATA 300

Query: 301 NNGDDTGTEAPHANSDVKCFSEDEEANEASKDDVSVEISAEEINDSDKLSQPTEDGDLTE 360
           NNGDDTGTEAPHANSDVKCFSEDEEANEASKDDVSVEISAEEINDSDKLSQPTEDGDLTE
Sbjct: 301 NNGDDTGTEAPHANSDVKCFSEDEEANEASKDDVSVEISAEEINDSDKLSQPTEDGDLTE 360

Query: 361 VVDNSIIVEESGMEVESKQNESTCELKHIIEEVINEEVKLMNDTAEKLSLPHENVEEEAQ 420
           VVDNSIIVEESGMEVESKQNESTCELKHIIEEVINEEVKLMNDTAEKLSLPHENVEEEAQ
Sbjct: 361 VVDNSIIVEESGMEVESKQNESTCELKHIIEEVINEEVKLMNDTAEKLSLPHENVEEEAQ 420

Query: 421 VDDDVLSSDEESVMEVKDDQTQVEAENESTYELEHIIEKVNEEEVKLMKDTAEELSLPHE 480
           VDDDVLSSDEESVMEVKDDQTQVEAENESTYELEHIIEKVNEEEVKLMKDTAEELSLPHE
Sbjct: 421 VDDDVLSSDEESVMEVKDDQTQVEAENESTYELEHIIEKVNEEEVKLMKDTAEELSLPHE 480

Query: 481 SVEEKTQVDDGVLSSDEESVIEVKDDQDEYDYESDPTIEEEASDADIEVDKKETEMEKVS 540
           SVEEKTQVDDGVLSSDEESVIEVKDDQDEYDYESDPTIEEEASDADIEVDKKETEMEKVS
Sbjct: 481 SVEEKTQVDDGVLSSDEESVIEVKDDQDEYDYESDPTIEEEASDADIEVDKKETEMEKVS 540

Query: 541 VQAEEDDTTINTSMEEFPENVSEDLVDVKIQWQMEEEAPSKDIDVDAIDSQPKIPNDNVQ 600
           VQAEEDDTTINTSMEEFPENVSEDLVDVKIQWQMEEEAPSKDIDVDAIDSQPKIPNDNVQ
Sbjct: 541 VQAEEDDTTINTSMEEFPENVSEDLVDVKIQWQMEEEAPSKDIDVDAIDSQPKIPNDNVQ 600

Query: 601 CEEALVEIIPTSSNNNGVQSLPSFGADQLALATQFPRPTVGTKSPVREQTIRLLMDNSDA 660
           CEEALVEIIPTSSNNNGVQSLPSFGADQLALATQFPRPTVGTKSPVREQTIRLLMDNSDA
Sbjct: 601 CEEALVEIIPTSSNNNGVQSLPSFGADQLALATQFPRPTVGTKSPVREQTIRLLMDNSDA 660

Query: 661 EEEEDGKEQEKANCIEKQIVETNDMSLRQLKKMFKEKLQLSKQKMDNNSINSKVVGGGGK 720
           EEEEDGKEQEKANCIEKQIVETNDMSLRQLKKMFKEKLQLSKQKMDNNSINSKVVGGGGK
Sbjct: 661 EEEEDGKEQEKANCIEKQIVETNDMSLRQLKKMFKEKLQLSKQKMDNNSINSKVVGGGGK 720

Query: 721 VRTALQPVPENIMTINELERRF 743
           VRTALQPVPENIMTINELERRF
Sbjct: 721 VRTALQPVPENIMTINELERRF 742

BLAST of Bhi12G000502 vs. NCBI nr
Match: XP_038906923.1 (interaptin-like isoform X2 [Benincasa hispida])

HSP 1 Score: 1327.8 bits (3435), Expect = 0.0e+00
Identity = 739/742 (99.60%), Postives = 739/742 (99.60%), Query Frame = 0

Query: 1   MDFRTLSRKELQALCKRNKIPANITNVAMADALAALSSVEGIEEFFNGERSGVPESPMKP 60
           MDFRTLSRKELQALCKRNKIPANITNVAMADALAALSSVEGIEEFFNGERSGVPESPMKP
Sbjct: 1   MDFRTLSRKELQALCKRNKIPANITNVAMADALAALSSVEGIEEFFNGERSGVPESPMKP 60

Query: 61  EVISSEIPRTALRTSTRRKAVKDEAITTRTRRAAAARDTEESENRDLNVALTTPSLPGSR 120
           EVISSEIPRTALRTSTRRKAVKDEAITTRTRRAAAARDTEESENRDLNVALTTPSLPGSR
Sbjct: 61  EVISSEIPRTALRTSTRRKAVKDEAITTRTRRAAAARDTEESENRDLNVALTTPSLPGSR 120

Query: 121 RRTAAASSACKKVDFQMTVDDQKEDNDLDKKKKAIEKTPAVPKSLRVAGASTRKRTETRN 180
           RRTAAASSACKKVDFQMTVDDQKEDNDLDKKKKAIEKTPAVPKSLRVAGASTRKRTETRN
Sbjct: 121 RRTAAASSACKKVDFQMTVDDQKEDNDLDKKKKAIEKTPAVPKSLRVAGASTRKRTETRN 180

Query: 181 NGAAEQRVYSTRRSVRFLEKNMESLSLGEDREMESITVHMSFDDMPNSSVGAGPVKEDTE 240
           NGAAEQRVYSTRRSVRFLEKNMESLSLGEDREMESITVHMSFDDMPNSS   GPVKEDTE
Sbjct: 181 NGAAEQRVYSTRRSVRFLEKNMESLSLGEDREMESITVHMSFDDMPNSS---GPVKEDTE 240

Query: 241 LETESKKSDESESKLDKDTGVEIDDQEKNEMESEVELSEEEQEMVLDDPLKSSEEKPATA 300
           LETESKKSDESESKLDKDTGVEIDDQEKNEMESEVELSEEEQEMVLDDPLKSSEEKPATA
Sbjct: 241 LETESKKSDESESKLDKDTGVEIDDQEKNEMESEVELSEEEQEMVLDDPLKSSEEKPATA 300

Query: 301 NNGDDTGTEAPHANSDVKCFSEDEEANEASKDDVSVEISAEEINDSDKLSQPTEDGDLTE 360
           NNGDDTGTEAPHANSDVKCFSEDEEANEASKDDVSVEISAEEINDSDKLSQPTEDGDLTE
Sbjct: 301 NNGDDTGTEAPHANSDVKCFSEDEEANEASKDDVSVEISAEEINDSDKLSQPTEDGDLTE 360

Query: 361 VVDNSIIVEESGMEVESKQNESTCELKHIIEEVINEEVKLMNDTAEKLSLPHENVEEEAQ 420
           VVDNSIIVEESGMEVESKQNESTCELKHIIEEVINEEVKLMNDTAEKLSLPHENVEEEAQ
Sbjct: 361 VVDNSIIVEESGMEVESKQNESTCELKHIIEEVINEEVKLMNDTAEKLSLPHENVEEEAQ 420

Query: 421 VDDDVLSSDEESVMEVKDDQTQVEAENESTYELEHIIEKVNEEEVKLMKDTAEELSLPHE 480
           VDDDVLSSDEESVMEVKDDQTQVEAENESTYELEHIIEKVNEEEVKLMKDTAEELSLPHE
Sbjct: 421 VDDDVLSSDEESVMEVKDDQTQVEAENESTYELEHIIEKVNEEEVKLMKDTAEELSLPHE 480

Query: 481 SVEEKTQVDDGVLSSDEESVIEVKDDQDEYDYESDPTIEEEASDADIEVDKKETEMEKVS 540
           SVEEKTQVDDGVLSSDEESVIEVKDDQDEYDYESDPTIEEEASDADIEVDKKETEMEKVS
Sbjct: 481 SVEEKTQVDDGVLSSDEESVIEVKDDQDEYDYESDPTIEEEASDADIEVDKKETEMEKVS 540

Query: 541 VQAEEDDTTINTSMEEFPENVSEDLVDVKIQWQMEEEAPSKDIDVDAIDSQPKIPNDNVQ 600
           VQAEEDDTTINTSMEEFPENVSEDLVDVKIQWQMEEEAPSKDIDVDAIDSQPKIPNDNVQ
Sbjct: 541 VQAEEDDTTINTSMEEFPENVSEDLVDVKIQWQMEEEAPSKDIDVDAIDSQPKIPNDNVQ 600

Query: 601 CEEALVEIIPTSSNNNGVQSLPSFGADQLALATQFPRPTVGTKSPVREQTIRLLMDNSDA 660
           CEEALVEIIPTSSNNNGVQSLPSFGADQLALATQFPRPTVGTKSPVREQTIRLLMDNSDA
Sbjct: 601 CEEALVEIIPTSSNNNGVQSLPSFGADQLALATQFPRPTVGTKSPVREQTIRLLMDNSDA 660

Query: 661 EEEEDGKEQEKANCIEKQIVETNDMSLRQLKKMFKEKLQLSKQKMDNNSINSKVVGGGGK 720
           EEEEDGKEQEKANCIEKQIVETNDMSLRQLKKMFKEKLQLSKQKMDNNSINSKVVGGGGK
Sbjct: 661 EEEEDGKEQEKANCIEKQIVETNDMSLRQLKKMFKEKLQLSKQKMDNNSINSKVVGGGGK 720

Query: 721 VRTALQPVPENIMTINELERRF 743
           VRTALQPVPENIMTINELERRF
Sbjct: 721 VRTALQPVPENIMTINELERRF 739

BLAST of Bhi12G000502 vs. NCBI nr
Match: XP_008467303.1 (PREDICTED: probable beta-tubulin polyglutamylase [Cucumis melo])

HSP 1 Score: 858.2 bits (2216), Expect = 5.2e-245
Identity = 530/742 (71.43%), Postives = 577/742 (77.76%), Query Frame = 0

Query: 1   MDFRTLSRKELQALCKRNKIPANITNVAMADALAALSSVEGIEEFFNGERSGVPESPMKP 60
           MDF TLSR+ELQALCKRNKIPANITNVAMADALAAL SVEGIEEF NG+RSGV ESPMK 
Sbjct: 1   MDFHTLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFLNGDRSGVSESPMKV 60

Query: 61  EVISSEIPRTALRTSTRRKAVKDEAITTRTRRAAAARDTEESENRDLNVALTTPSLPGSR 120
           EVIS EIPRTALRTSTRRKAVKDE ITTR+RR A AR TEESENRD N+ALTTPSLPGSR
Sbjct: 61  EVISLEIPRTALRTSTRRKAVKDETITTRSRRGAVARGTEESENRDFNMALTTPSLPGSR 120

Query: 121 RRTAAASSACKKVDFQMTVDDQKEDNDLDKKKKAIEKTPAVPKS-LRVAGASTRKRTETR 180
           RRTAAASSACKKVDFQMTVDDQKEDNDLD++KK IEKTPAVPKS  RV GASTRKRTETR
Sbjct: 121 RRTAAASSACKKVDFQMTVDDQKEDNDLDQEKKEIEKTPAVPKSQKRVVGASTRKRTETR 180

Query: 181 NNGAAEQRVYSTRRSVRFLEKNMESLSLGEDREMESITVHMSFDDMPNSSVGAGPVKEDT 240
           NNGAAEQRVYSTRRSVR LEKNMESLSLG D EME I+VHMSF+DMPNSSV   PVKE+T
Sbjct: 181 NNGAAEQRVYSTRRSVRLLEKNMESLSLGGDEEMEPISVHMSFEDMPNSSV---PVKENT 240

Query: 241 ELETESKKSDESESKLDKDTGVEIDDQEKNEMESEVELSEEEQEMVLDDPLKSSEEKPAT 300
           ELE ESK  DESESKLD+D  VE+DD EK+E+ SEV+LSEEE E+VLDD LKSS+E   T
Sbjct: 241 ELEIESKIIDESESKLDEDLRVEVDDGEKSEIGSEVKLSEEEPEIVLDDLLKSSDENVVT 300

Query: 301 ANNGDDTGTEAPHANSDVKCFSEDEEANEASKDDVSVEISAEEINDSDKLSQPTEDGDLT 360
           A + DD+ TEA HAN DVKCFSED + NEASK   +VEISAEEIN+SDK S PTED D+T
Sbjct: 301 AKSVDDSETEALHANLDVKCFSED-KVNEASK---AVEISAEEINESDKSSLPTEDLDVT 360

Query: 361 EVVDNSIIVEESGMEVESKQNESTCELKHIIEEVINEEVKLMNDTAEKLSLPHENVEEEA 420
           EVV  S I EE GMEVE KQNEST EL+H IEE + EE K +N++AE+LSLPHENVEEE 
Sbjct: 361 EVVHKSNIAEEFGMEVECKQNESTSELEHKIEEDVEEE-KHVNNSAEELSLPHENVEEET 420

Query: 421 QVDDDVLSSDEESVMEVKDDQTQVEAENESTYELEHIIEKVNEEEVKLMKDTAEELSLPH 480
           +VD                                                         
Sbjct: 421 EVDG-------------------------------------------------------- 480

Query: 481 ESVEEKTQVDDGVLSSDEESVIEVKDDQDEYDYESDPTIEEEASDADIEVDKKETEMEKV 540
                    DD VLSSDEES++EVKDDQDEYDY+SDPT E+EASDADIE DKKETEMEK 
Sbjct: 481 ---------DDDVLSSDEESLMEVKDDQDEYDYKSDPTSEDEASDADIEGDKKETEMEKS 540

Query: 541 SVQAEEDDTTINTSMEEFPENVSEDLVDVKIQWQMEEEAPSKDIDVDAIDSQPKIPNDNV 600
           SVQAEEDDT IN S+EEFPENV++DLVDVKIQWQMEEEAP KDI V+AIDSQPKIPN  V
Sbjct: 541 SVQAEEDDTIINKSVEEFPENVADDLVDVKIQWQMEEEAPPKDITVEAIDSQPKIPNAIV 600

Query: 601 QCEEALVEIIPTSSNNNGVQSLPSFGADQLALATQFPRPTVGTKSPVREQTIRLLMDNSD 660
           QCEEALVEI  TSSNNN VQS          LA QFPRPT+  KSPV+EQ I LLMDN D
Sbjct: 601 QCEEALVEITTTSSNNNAVQS----------LANQFPRPTIEAKSPVKEQIISLLMDNLD 656

Query: 661 AEEEEDGKEQEKANCIEKQIVETNDMSLRQLKKMFKEKLQLSKQKMDNNSINSKVVGGGG 720
           A EEED KEQ KA CI  QIVE ND+SLRQLKKMFKEKLQL+K+KMDNN+IN+KVVGGG 
Sbjct: 661 A-EEEDEKEQNKAKCI--QIVEKNDVSLRQLKKMFKEKLQLNKKKMDNNNINTKVVGGGV 656

Query: 721 KVRTALQPVPENIMTINELERR 742
           KVRTALQPV EN MT++ELE+R
Sbjct: 721 KVRTALQPVLENRMTVDELEKR 656

BLAST of Bhi12G000502 vs. NCBI nr
Match: XP_031742149.1 (probable serine/threonine-protein kinase kinX isoform X1 [Cucumis sativus])

HSP 1 Score: 845.1 bits (2182), Expect = 4.5e-241
Identity = 519/743 (69.85%), Postives = 571/743 (76.85%), Query Frame = 0

Query: 1   MDFRTLSRKELQALCKRNKIPANITNVAMADALAALSSVEGIEEFFNGERSGVPESPMKP 60
           MDF TLSR+ELQALCKRNKIPANITNVAMADALAAL SVEGIEEF NG+RSGVPESPMK 
Sbjct: 1   MDFYTLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFLNGDRSGVPESPMKG 60

Query: 61  EVISSEIPRTALRTSTRRKAVKDEAITTRTRRAAAARDTEESENRDLNVALTTPSLPGSR 120
           EVISSEIPRTALRTSTRRKAVKDE ITTR+RR AAARDTEESENRDLN+ALTTPSLPGSR
Sbjct: 61  EVISSEIPRTALRTSTRRKAVKDETITTRSRRGAAARDTEESENRDLNMALTTPSLPGSR 120

Query: 121 RRTAAASSACKKVDFQMTVDDQKEDNDLDKKKKAIEKTPAVPKS-LRVAGASTRKRTETR 180
           RRTAAASSACKKVDFQMTVDDQKED DLD++KK IE TPAVPKS  RVAGASTRKRTET+
Sbjct: 121 RRTAAASSACKKVDFQMTVDDQKEDKDLDQEKKEIENTPAVPKSQKRVAGASTRKRTETK 180

Query: 181 NNGAAEQRVYSTRRSVRFLEKNMESLSLGEDREMESITVHMSFDDMPN-SSVGAGPVKED 240
           ++GAAEQRVYSTRRSVR LEKNMES+SLG D +ME ITVHMSFDDMPN S++GA PVKE+
Sbjct: 181 DSGAAEQRVYSTRRSVRLLEKNMESMSLGGDEKMEPITVHMSFDDMPNISAIGAEPVKEN 240

Query: 241 TELETESKKSDESESKLDKDTGVEIDDQEKNEMESEVELSEEEQEMVLDDPLKSSEEKPA 300
            ELETESKK+DES SKLD+D  VE+DD EKNEM +EV+LSEEE E+VLDD LKS +E   
Sbjct: 241 MELETESKKTDESVSKLDEDLSVEVDDGEKNEMGTEVKLSEEEPEIVLDDLLKSFDENVI 300

Query: 301 TANNGDDTGTEAPHANSDVKCFSEDEEANEASKDDVSVEISAEEINDSDKLSQPTEDGDL 360
           +A + DD+ TEAPHANSDVKCFSEDE+ NE SKDD SVE SAEEIN+SDK S PTED D+
Sbjct: 301 SAKSVDDSITEAPHANSDVKCFSEDEKVNEVSKDDDSVESSAEEINESDKSSLPTEDLDV 360

Query: 361 TEVVDNSIIVEESGMEVESKQNESTCELKHIIEEVINEEVKLMNDTAEKLSLPHENVEEE 420
           TE V  S I EE G E ES+QNESTCEL H IEE + EE K +N++AE+LSLPHENVEEE
Sbjct: 361 TE-VHKSSIAEEFGKEFESQQNESTCELDHKIEEDVEEE-KHVNNSAEELSLPHENVEEE 420

Query: 421 AQVDDDVLSSDEESVMEVKDDQTQVEAENESTYELEHIIEKVNEEEVKLMKDTAEELSLP 480
            +V DDVLSSD+ES                                              
Sbjct: 421 TEVGDDVLSSDKES---------------------------------------------- 480

Query: 481 HESVEEKTQVDDGVLSSDEESVIEVKDDQDEYDYESDPTIEEEASDADIEVDKKETEMEK 540
                                 +EVKDDQDE DY+SDPT   EASDADIE DKKETEMEK
Sbjct: 481 ----------------------LEVKDDQDENDYKSDPTTGNEASDADIEGDKKETEMEK 540

Query: 541 VSVQAEEDDTTINTSMEEFPENVSEDLVDVKIQWQMEEEAPSKDIDVDAIDSQPKIPNDN 600
            SVQAEEDDT IN SMEEFPEN S+DLVDVKIQW++EEE   KDI V+AIDSQP+IPN  
Sbjct: 541 SSVQAEEDDTIINESMEEFPENASDDLVDVKIQWKVEEETLPKDITVEAIDSQPEIPNAI 600

Query: 601 VQCEEALVEIIPTSSNNNGVQSLPSFGADQLALATQFPRPTVGTKSPVREQTIRLLMDNS 660
           VQCEEALVEI  TSSNNN VQS          LATQFPRPT+  KSPV+EQTI LLMDN 
Sbjct: 601 VQCEEALVEITTTSSNNNAVQS----------LATQFPRPTIEAKSPVKEQTISLLMDNP 660

Query: 661 DAEEEEDGKEQEKANCIEKQIVETNDMSLRQLKKMFKEKLQLSKQKMDNNSINSKVVGGG 720
           D EEEE  KEQ   N    Q V  ND SLRQLKK+FKEKLQL+ +KMDNN+IN+KVVGGG
Sbjct: 661 DVEEEEAEKEQ---NPKFVQFVVKNDTSLRQLKKLFKEKLQLNNKKMDNNNINTKVVGGG 660

Query: 721 GKVRTALQPVPENIMTINELERR 742
            KVRTALQPVPEN MT++ELE+R
Sbjct: 721 VKVRTALQPVPENRMTVDELEKR 660

BLAST of Bhi12G000502 vs. NCBI nr
Match: KAA0055115.1 (putative beta-tubulin polyglutamylase [Cucumis melo var. makuwa])

HSP 1 Score: 844.0 bits (2179), Expect = 1.0e-240
Identity = 521/742 (70.22%), Postives = 573/742 (77.22%), Query Frame = 0

Query: 1   MDFRTLSRKELQALCKRNKIPANITNVAMADALAALSSVEGIEEFFNGERSGVPESPMKP 60
           MDF TLSR+ELQALCKRNKIPANITNVAMADALAAL SVEGIEEF NG+RSGVPESPMK 
Sbjct: 1   MDFHTLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFLNGDRSGVPESPMKV 60

Query: 61  EVISSEIPRTALRTSTRRKAVKDEAITTRTRRAAAARDTEESENRDLNVALTTPSLPGSR 120
           EVIS EIPRTALRTSTRRKAVKDE IT R+RR A AR TEESENRDLN+ALTTPSLPGSR
Sbjct: 61  EVISLEIPRTALRTSTRRKAVKDETITIRSRRGAVARGTEESENRDLNMALTTPSLPGSR 120

Query: 121 RRTAAASSACKKVDFQMTVDDQKEDNDLDKKKKAIEKTPAVPKS-LRVAGASTRKRTETR 180
           RRTAAASSACKKVDFQMT+DDQKEDNDLD++KK IEKTPAVPKS  RV GASTRKRTET+
Sbjct: 121 RRTAAASSACKKVDFQMTIDDQKEDNDLDQEKKEIEKTPAVPKSQKRVVGASTRKRTETK 180

Query: 181 NNGAAEQRVYSTRRSVRFLEKNMESLSLGEDREMESITVHMSFDDMPNSSVGAGPVKEDT 240
           NNGAAEQRVYSTRRSVR LEKNMESLSLG D EME I+VHMSF+DMPNSSV   PVKE+T
Sbjct: 181 NNGAAEQRVYSTRRSVRLLEKNMESLSLGGDEEMEPISVHMSFEDMPNSSV---PVKENT 240

Query: 241 ELETESKKSDESESKLDKDTGVEIDDQEKNEMESEVELSEEEQEMVLDDPLKSSEEKPAT 300
           +LE ESK  DESESKLD+D  VE+DD EK+E+ SEV+LSEEE E+VLDD LKSS+E   T
Sbjct: 241 KLEIESKIIDESESKLDEDLRVEVDDGEKSEIGSEVKLSEEEPEIVLDDLLKSSDENVVT 300

Query: 301 ANNGDDTGTEAPHANSDVKCFSEDEEANEASKDDVSVEISAEEINDSDKLSQPTEDGDLT 360
           A + DD+ TEA HAN DVKCFSED + NEASK   +VEISAEEIN+SDK S PTED D+T
Sbjct: 301 AKSVDDSETEALHANLDVKCFSED-KVNEASK---AVEISAEEINESDKSSLPTEDLDVT 360

Query: 361 EVVDNSIIVEESGMEVESKQNESTCELKHIIEEVINEEVKLMNDTAEKLSLPHENVEEEA 420
           EVV  S I EE GMEVE KQNEST EL+H IEE + EE K +N++AE+L LPHENVEEE 
Sbjct: 361 EVVHKSNIAEEFGMEVECKQNESTSELEHKIEEDVEEE-KHVNNSAEELYLPHENVEEET 420

Query: 421 QVDDDVLSSDEESVMEVKDDQTQVEAENESTYELEHIIEKVNEEEVKLMKDTAEELSLPH 480
           +VD                                                         
Sbjct: 421 EVDG-------------------------------------------------------- 480

Query: 481 ESVEEKTQVDDGVLSSDEESVIEVKDDQDEYDYESDPTIEEEASDADIEVDKKETEMEKV 540
                    DD VLSSDEES++EVKDDQDEYDY+SDPT E+EASDA +E DKKETE EK 
Sbjct: 481 ---------DDDVLSSDEESLMEVKDDQDEYDYKSDPTSEDEASDAAVEGDKKETEREKS 540

Query: 541 SVQAEEDDTTINTSMEEFPENVSEDLVDVKIQWQMEEEAPSKDIDVDAIDSQPKIPNDNV 600
           SVQAEEDDT IN S+EEFP NV++DLVDVKIQWQMEEEA  KDI V+AIDSQPKIPN  V
Sbjct: 541 SVQAEEDDTIINKSVEEFPANVADDLVDVKIQWQMEEEATPKDITVEAIDSQPKIPNAIV 600

Query: 601 QCEEALVEIIPTSSNNNGVQSLPSFGADQLALATQFPRPTVGTKSPVREQTIRLLMDNSD 660
           QCEEALVEI  TSSNNN VQS          LA QFPRPT+  KSPV+EQ   LLMDN D
Sbjct: 601 QCEEALVEITTTSSNNNAVQS----------LANQFPRPTIEAKSPVKEQISSLLMDNLD 656

Query: 661 AEEEEDGKEQEKANCIEKQIVETNDMSLRQLKKMFKEKLQLSKQKMDNNSINSKVVGGGG 720
           A EEED KEQ KA CI  QIVE ND+SLRQLKKMFKE+LQL+K+KMDNN+IN+KVVGGG 
Sbjct: 661 A-EEEDEKEQNKAKCI--QIVEKNDISLRQLKKMFKEQLQLNKKKMDNNNINTKVVGGGV 656

Query: 721 KVRTALQPVPENIMTINELERR 742
           KVRTALQPVPEN MT++ELE+R
Sbjct: 721 KVRTALQPVPENRMTVDELEKR 656

BLAST of Bhi12G000502 vs. ExPASy TrEMBL
Match: A0A1S3CT90 (probable beta-tubulin polyglutamylase OS=Cucumis melo OX=3656 GN=LOC103504683 PE=4 SV=1)

HSP 1 Score: 858.2 bits (2216), Expect = 2.5e-245
Identity = 530/742 (71.43%), Postives = 577/742 (77.76%), Query Frame = 0

Query: 1   MDFRTLSRKELQALCKRNKIPANITNVAMADALAALSSVEGIEEFFNGERSGVPESPMKP 60
           MDF TLSR+ELQALCKRNKIPANITNVAMADALAAL SVEGIEEF NG+RSGV ESPMK 
Sbjct: 1   MDFHTLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFLNGDRSGVSESPMKV 60

Query: 61  EVISSEIPRTALRTSTRRKAVKDEAITTRTRRAAAARDTEESENRDLNVALTTPSLPGSR 120
           EVIS EIPRTALRTSTRRKAVKDE ITTR+RR A AR TEESENRD N+ALTTPSLPGSR
Sbjct: 61  EVISLEIPRTALRTSTRRKAVKDETITTRSRRGAVARGTEESENRDFNMALTTPSLPGSR 120

Query: 121 RRTAAASSACKKVDFQMTVDDQKEDNDLDKKKKAIEKTPAVPKS-LRVAGASTRKRTETR 180
           RRTAAASSACKKVDFQMTVDDQKEDNDLD++KK IEKTPAVPKS  RV GASTRKRTETR
Sbjct: 121 RRTAAASSACKKVDFQMTVDDQKEDNDLDQEKKEIEKTPAVPKSQKRVVGASTRKRTETR 180

Query: 181 NNGAAEQRVYSTRRSVRFLEKNMESLSLGEDREMESITVHMSFDDMPNSSVGAGPVKEDT 240
           NNGAAEQRVYSTRRSVR LEKNMESLSLG D EME I+VHMSF+DMPNSSV   PVKE+T
Sbjct: 181 NNGAAEQRVYSTRRSVRLLEKNMESLSLGGDEEMEPISVHMSFEDMPNSSV---PVKENT 240

Query: 241 ELETESKKSDESESKLDKDTGVEIDDQEKNEMESEVELSEEEQEMVLDDPLKSSEEKPAT 300
           ELE ESK  DESESKLD+D  VE+DD EK+E+ SEV+LSEEE E+VLDD LKSS+E   T
Sbjct: 241 ELEIESKIIDESESKLDEDLRVEVDDGEKSEIGSEVKLSEEEPEIVLDDLLKSSDENVVT 300

Query: 301 ANNGDDTGTEAPHANSDVKCFSEDEEANEASKDDVSVEISAEEINDSDKLSQPTEDGDLT 360
           A + DD+ TEA HAN DVKCFSED + NEASK   +VEISAEEIN+SDK S PTED D+T
Sbjct: 301 AKSVDDSETEALHANLDVKCFSED-KVNEASK---AVEISAEEINESDKSSLPTEDLDVT 360

Query: 361 EVVDNSIIVEESGMEVESKQNESTCELKHIIEEVINEEVKLMNDTAEKLSLPHENVEEEA 420
           EVV  S I EE GMEVE KQNEST EL+H IEE + EE K +N++AE+LSLPHENVEEE 
Sbjct: 361 EVVHKSNIAEEFGMEVECKQNESTSELEHKIEEDVEEE-KHVNNSAEELSLPHENVEEET 420

Query: 421 QVDDDVLSSDEESVMEVKDDQTQVEAENESTYELEHIIEKVNEEEVKLMKDTAEELSLPH 480
           +VD                                                         
Sbjct: 421 EVDG-------------------------------------------------------- 480

Query: 481 ESVEEKTQVDDGVLSSDEESVIEVKDDQDEYDYESDPTIEEEASDADIEVDKKETEMEKV 540
                    DD VLSSDEES++EVKDDQDEYDY+SDPT E+EASDADIE DKKETEMEK 
Sbjct: 481 ---------DDDVLSSDEESLMEVKDDQDEYDYKSDPTSEDEASDADIEGDKKETEMEKS 540

Query: 541 SVQAEEDDTTINTSMEEFPENVSEDLVDVKIQWQMEEEAPSKDIDVDAIDSQPKIPNDNV 600
           SVQAEEDDT IN S+EEFPENV++DLVDVKIQWQMEEEAP KDI V+AIDSQPKIPN  V
Sbjct: 541 SVQAEEDDTIINKSVEEFPENVADDLVDVKIQWQMEEEAPPKDITVEAIDSQPKIPNAIV 600

Query: 601 QCEEALVEIIPTSSNNNGVQSLPSFGADQLALATQFPRPTVGTKSPVREQTIRLLMDNSD 660
           QCEEALVEI  TSSNNN VQS          LA QFPRPT+  KSPV+EQ I LLMDN D
Sbjct: 601 QCEEALVEITTTSSNNNAVQS----------LANQFPRPTIEAKSPVKEQIISLLMDNLD 656

Query: 661 AEEEEDGKEQEKANCIEKQIVETNDMSLRQLKKMFKEKLQLSKQKMDNNSINSKVVGGGG 720
           A EEED KEQ KA CI  QIVE ND+SLRQLKKMFKEKLQL+K+KMDNN+IN+KVVGGG 
Sbjct: 661 A-EEEDEKEQNKAKCI--QIVEKNDVSLRQLKKMFKEKLQLNKKKMDNNNINTKVVGGGV 656

Query: 721 KVRTALQPVPENIMTINELERR 742
           KVRTALQPV EN MT++ELE+R
Sbjct: 721 KVRTALQPVLENRMTVDELEKR 656

BLAST of Bhi12G000502 vs. ExPASy TrEMBL
Match: A0A5A7UN97 (Putative beta-tubulin polyglutamylase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00410 PE=4 SV=1)

HSP 1 Score: 844.0 bits (2179), Expect = 4.9e-241
Identity = 521/742 (70.22%), Postives = 573/742 (77.22%), Query Frame = 0

Query: 1   MDFRTLSRKELQALCKRNKIPANITNVAMADALAALSSVEGIEEFFNGERSGVPESPMKP 60
           MDF TLSR+ELQALCKRNKIPANITNVAMADALAAL SVEGIEEF NG+RSGVPESPMK 
Sbjct: 1   MDFHTLSRRELQALCKRNKIPANITNVAMADALAALQSVEGIEEFLNGDRSGVPESPMKV 60

Query: 61  EVISSEIPRTALRTSTRRKAVKDEAITTRTRRAAAARDTEESENRDLNVALTTPSLPGSR 120
           EVIS EIPRTALRTSTRRKAVKDE IT R+RR A AR TEESENRDLN+ALTTPSLPGSR
Sbjct: 61  EVISLEIPRTALRTSTRRKAVKDETITIRSRRGAVARGTEESENRDLNMALTTPSLPGSR 120

Query: 121 RRTAAASSACKKVDFQMTVDDQKEDNDLDKKKKAIEKTPAVPKS-LRVAGASTRKRTETR 180
           RRTAAASSACKKVDFQMT+DDQKEDNDLD++KK IEKTPAVPKS  RV GASTRKRTET+
Sbjct: 121 RRTAAASSACKKVDFQMTIDDQKEDNDLDQEKKEIEKTPAVPKSQKRVVGASTRKRTETK 180

Query: 181 NNGAAEQRVYSTRRSVRFLEKNMESLSLGEDREMESITVHMSFDDMPNSSVGAGPVKEDT 240
           NNGAAEQRVYSTRRSVR LEKNMESLSLG D EME I+VHMSF+DMPNSSV   PVKE+T
Sbjct: 181 NNGAAEQRVYSTRRSVRLLEKNMESLSLGGDEEMEPISVHMSFEDMPNSSV---PVKENT 240

Query: 241 ELETESKKSDESESKLDKDTGVEIDDQEKNEMESEVELSEEEQEMVLDDPLKSSEEKPAT 300
           +LE ESK  DESESKLD+D  VE+DD EK+E+ SEV+LSEEE E+VLDD LKSS+E   T
Sbjct: 241 KLEIESKIIDESESKLDEDLRVEVDDGEKSEIGSEVKLSEEEPEIVLDDLLKSSDENVVT 300

Query: 301 ANNGDDTGTEAPHANSDVKCFSEDEEANEASKDDVSVEISAEEINDSDKLSQPTEDGDLT 360
           A + DD+ TEA HAN DVKCFSED + NEASK   +VEISAEEIN+SDK S PTED D+T
Sbjct: 301 AKSVDDSETEALHANLDVKCFSED-KVNEASK---AVEISAEEINESDKSSLPTEDLDVT 360

Query: 361 EVVDNSIIVEESGMEVESKQNESTCELKHIIEEVINEEVKLMNDTAEKLSLPHENVEEEA 420
           EVV  S I EE GMEVE KQNEST EL+H IEE + EE K +N++AE+L LPHENVEEE 
Sbjct: 361 EVVHKSNIAEEFGMEVECKQNESTSELEHKIEEDVEEE-KHVNNSAEELYLPHENVEEET 420

Query: 421 QVDDDVLSSDEESVMEVKDDQTQVEAENESTYELEHIIEKVNEEEVKLMKDTAEELSLPH 480
           +VD                                                         
Sbjct: 421 EVDG-------------------------------------------------------- 480

Query: 481 ESVEEKTQVDDGVLSSDEESVIEVKDDQDEYDYESDPTIEEEASDADIEVDKKETEMEKV 540
                    DD VLSSDEES++EVKDDQDEYDY+SDPT E+EASDA +E DKKETE EK 
Sbjct: 481 ---------DDDVLSSDEESLMEVKDDQDEYDYKSDPTSEDEASDAAVEGDKKETEREKS 540

Query: 541 SVQAEEDDTTINTSMEEFPENVSEDLVDVKIQWQMEEEAPSKDIDVDAIDSQPKIPNDNV 600
           SVQAEEDDT IN S+EEFP NV++DLVDVKIQWQMEEEA  KDI V+AIDSQPKIPN  V
Sbjct: 541 SVQAEEDDTIINKSVEEFPANVADDLVDVKIQWQMEEEATPKDITVEAIDSQPKIPNAIV 600

Query: 601 QCEEALVEIIPTSSNNNGVQSLPSFGADQLALATQFPRPTVGTKSPVREQTIRLLMDNSD 660
           QCEEALVEI  TSSNNN VQS          LA QFPRPT+  KSPV+EQ   LLMDN D
Sbjct: 601 QCEEALVEITTTSSNNNAVQS----------LANQFPRPTIEAKSPVKEQISSLLMDNLD 656

Query: 661 AEEEEDGKEQEKANCIEKQIVETNDMSLRQLKKMFKEKLQLSKQKMDNNSINSKVVGGGG 720
           A EEED KEQ KA CI  QIVE ND+SLRQLKKMFKE+LQL+K+KMDNN+IN+KVVGGG 
Sbjct: 661 A-EEEDEKEQNKAKCI--QIVEKNDISLRQLKKMFKEQLQLNKKKMDNNNINTKVVGGGV 656

Query: 721 KVRTALQPVPENIMTINELERR 742
           KVRTALQPVPEN MT++ELE+R
Sbjct: 721 KVRTALQPVPENRMTVDELEKR 656

BLAST of Bhi12G000502 vs. ExPASy TrEMBL
Match: A0A6J1I214 (uncharacterized protein LOC111469129 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111469129 PE=4 SV=1)

HSP 1 Score: 622.1 bits (1603), Expect = 3.0e-174
Identity = 426/746 (57.10%), Postives = 481/746 (64.48%), Query Frame = 0

Query: 1   MDFRTLSRKELQALCKRNKIPANITNVAMADALAALSSVEGIEEFFNGERSGVPESPMKP 60
           MDF +LSR+ELQ LCKRNKIPANITNVAMADALAAL  VEGIEEF + ERSGVPESPMK 
Sbjct: 1   MDFHSLSRRELQTLCKRNKIPANITNVAMADALAALPLVEGIEEFLSSERSGVPESPMKG 60

Query: 61  EVISSEIPRTALRTSTRRKAVKDEAITTRTRRAAAARDTEESENRDLNVALTTPSLPGSR 120
           EV+S EIPRT LRTSTRRK VKD+ ITT TRRAAAAR TEESENRDLNVALTTPSL   R
Sbjct: 61  EVVSFEIPRTELRTSTRRKTVKDDPITTLTRRAAAARGTEESENRDLNVALTTPSLRSGR 120

Query: 121 RRTAAASSACKKVDFQMTVDDQKEDNDLDKKKKAIEKTPAVPKSLR-VAGASTRKRTETR 180
           RRTA ASSACKKVDFQM VDD++E+NDLD +K  IE TPA PKS R VAG ST KRT+TR
Sbjct: 121 RRTAGASSACKKVDFQMAVDDEEEENDLDHEKPVIENTPAAPKSQRSVAGPSTSKRTQTR 180

Query: 181 NNGAAEQRVYSTRRSVRFLEKNMESLSLGEDREMESITVHMSFDDMPNSS-VGAGPVKED 240
           NNG  EQRVYSTRRSVR LE+ MESLSL EDR+M+ ITVHMSFD++PNSS +GAGPVKE+
Sbjct: 181 NNGVVEQRVYSTRRSVRLLEQTMESLSLAEDRKMKPITVHMSFDEIPNSSAIGAGPVKEE 240

Query: 241 TELETESKKSDESESKLDKDTGVEIDDQEKNEMESEVELSEEEQEMVLDDPLKSSEEKPA 300
           T+LE ESKK+DESESKLD+D G+E ++QEKNEM        EE +M  DD +K S +   
Sbjct: 241 TKLEVESKKTDESESKLDEDLGLETNEQEKNEM--------EEPQMDPDDSMKCSAD--- 300

Query: 301 TANNGDDTGTEAPHANSDVKCFSEDEEANEASKDDVSVEISAEEINDSDKLSQPTEDGDL 360
                                               SVEISAEEINDSDKLS P+ED DL
Sbjct: 301 ------------------------------------SVEISAEEINDSDKLSLPSEDVDL 360

Query: 361 TEVVDNSIIVEESGMEVESKQNESTCELKHIIEEVINEEVKLMNDTAEKLSLPHENVEEE 420
           TE V +S I EESGMEV+SK NES   L+HII     EEVK MN +AE+ SL  ENVEEE
Sbjct: 361 TE-VHHSSIEEESGMEVQSKYNESIFALEHII-----EEVKPMNISAEESSLHIENVEEE 420

Query: 421 AQVDDDVLSSDEESVMEVKDDQTQVEAENESTYELEHIIEKVNEEEVKLMKDTAEELSLP 480
            QVDD                                                       
Sbjct: 421 IQVDD------------------------------------------------------- 480

Query: 481 HESVEEKTQVDDGVLSSDEESVIEVKDDQDEYDYESDPTIEEEASDADIEVDKKETEMEK 540
                        VLS  EESV+EV DD+DE     +PTIEEE                 
Sbjct: 481 -------------VLSIVEESVMEVIDDEDEL----EPTIEEE----------------- 540

Query: 541 VSVQAEEDDTTINTSMEEFPENVSEDLVDVKIQWQMEEEAPSKDIDVDAIDSQPKIPNDN 600
                         S+ EFPENVSEDLVDVKIQ+QMEE  P+ D +VD IDS P IPND 
Sbjct: 541 --------------SVMEFPENVSEDLVDVKIQYQMEESPPT-DTNVDTIDSHPDIPNDI 573

Query: 601 VQCEEALVEIIPTSSNNNGVQSLPSFGADQLALATQFPRPT-VGTKSPVREQTIRLLMDN 660
           +QCEEAL+E +  SSNNNG+QSLPS  A QLALA QFPRP+ V TKS  +EQ  RLLMDN
Sbjct: 601 LQCEEALIE-VSVSSNNNGIQSLPSLAAAQLALANQFPRPSVVETKSSHKEQATRLLMDN 573

Query: 661 SDAEEEEDGKEQEKANCIEKQIVETNDMSLRQLKKMFKEKLQLSKQKMD-NNSINSKVVG 720
           SDAEEE        ANC +   VE NDMSLRQL+KMFKE+LQLSK+KMD NN+IN+    
Sbjct: 661 SDAEEE--------ANCTQNLTVEKNDMSLRQLRKMFKEQLQLSKKKMDNNNNINT---- 573

Query: 721 GGGKVRTALQPVPENIMTINELERRF 743
              KVRTALQP+PEN M ++ LE+ F
Sbjct: 721 ---KVRTALQPLPENRMAMDGLEKLF 573

BLAST of Bhi12G000502 vs. ExPASy TrEMBL
Match: A0A6J1HZT2 (uncharacterized protein LOC111469129 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111469129 PE=4 SV=1)

HSP 1 Score: 616.3 bits (1588), Expect = 1.7e-172
Identity = 424/745 (56.91%), Postives = 478/745 (64.16%), Query Frame = 0

Query: 1   MDFRTLSRKELQALCKRNKIPANITNVAMADALAALSSVEGIEEFFNGERSGVPESPMKP 60
           MDF +LSR+ELQ LCKRNKIPANITNVAMADALAAL  VEGIEEF + ERSGVPESPMK 
Sbjct: 1   MDFHSLSRRELQTLCKRNKIPANITNVAMADALAALPLVEGIEEFLSSERSGVPESPMKG 60

Query: 61  EVISSEIPRTALRTSTRRKAVKDEAITTRTRRAAAARDTEESENRDLNVALTTPSLPGSR 120
           EV+S EIPRT LRTSTRRK VKD+ ITT TRRAAAAR TEESENRDLNVALTTPSL   R
Sbjct: 61  EVVSFEIPRTELRTSTRRKTVKDDPITTLTRRAAAARGTEESENRDLNVALTTPSLRSGR 120

Query: 121 RRTAAASSACKKVDFQMTVDDQKEDNDLDKKKKAIEKTPAVPKSLR-VAGASTRKRTETR 180
           RRTA ASSACKKVDFQM VDD++E+NDLD +K  IE TPA PKS R VAG ST KRT+TR
Sbjct: 121 RRTAGASSACKKVDFQMAVDDEEEENDLDHEKPVIENTPAAPKSQRSVAGPSTSKRTQTR 180

Query: 181 NNGAAEQRVYSTRRSVRFLEKNMESLSLGEDREMESITVHMSFDDMPNSSVGAGPVKEDT 240
           NNG  EQRVYSTRRSVR LE+ MESLSL EDR+M+ ITVHMSFD++PNSS   GPVKE+T
Sbjct: 181 NNGVVEQRVYSTRRSVRLLEQTMESLSLAEDRKMKPITVHMSFDEIPNSS---GPVKEET 240

Query: 241 ELETESKKSDESESKLDKDTGVEIDDQEKNEMESEVELSEEEQEMVLDDPLKSSEEKPAT 300
           +LE ESKK+DESESKLD+D G+E ++QEKNEM        EE +M  DD +K S +    
Sbjct: 241 KLEVESKKTDESESKLDEDLGLETNEQEKNEM--------EEPQMDPDDSMKCSAD---- 300

Query: 301 ANNGDDTGTEAPHANSDVKCFSEDEEANEASKDDVSVEISAEEINDSDKLSQPTEDGDLT 360
                                              SVEISAEEINDSDKLS P+ED DLT
Sbjct: 301 -----------------------------------SVEISAEEINDSDKLSLPSEDVDLT 360

Query: 361 EVVDNSIIVEESGMEVESKQNESTCELKHIIEEVINEEVKLMNDTAEKLSLPHENVEEEA 420
           E V +S I EESGMEV+SK NES   L+HII     EEVK MN +AE+ SL  ENVEEE 
Sbjct: 361 E-VHHSSIEEESGMEVQSKYNESIFALEHII-----EEVKPMNISAEESSLHIENVEEEI 420

Query: 421 QVDDDVLSSDEESVMEVKDDQTQVEAENESTYELEHIIEKVNEEEVKLMKDTAEELSLPH 480
           QVDD                                                        
Sbjct: 421 QVDD-------------------------------------------------------- 480

Query: 481 ESVEEKTQVDDGVLSSDEESVIEVKDDQDEYDYESDPTIEEEASDADIEVDKKETEMEKV 540
                       VLS  EESV+EV DD+DE     +PTIEEE                  
Sbjct: 481 ------------VLSIVEESVMEVIDDEDEL----EPTIEEE------------------ 540

Query: 541 SVQAEEDDTTINTSMEEFPENVSEDLVDVKIQWQMEEEAPSKDIDVDAIDSQPKIPNDNV 600
                        S+ EFPENVSEDLVDVKIQ+QMEE  P+ D +VD IDS P IPND +
Sbjct: 541 -------------SVMEFPENVSEDLVDVKIQYQMEESPPT-DTNVDTIDSHPDIPNDIL 569

Query: 601 QCEEALVEIIPTSSNNNGVQSLPSFGADQLALATQFPRPT-VGTKSPVREQTIRLLMDNS 660
           QCEEAL+E +  SSNNNG+QSLPS  A QLALA QFPRP+ V TKS  +EQ  RLLMDNS
Sbjct: 601 QCEEALIE-VSVSSNNNGIQSLPSLAAAQLALANQFPRPSVVETKSSHKEQATRLLMDNS 569

Query: 661 DAEEEEDGKEQEKANCIEKQIVETNDMSLRQLKKMFKEKLQLSKQKMD-NNSINSKVVGG 720
           DAEEE        ANC +   VE NDMSLRQL+KMFKE+LQLSK+KMD NN+IN+     
Sbjct: 661 DAEEE--------ANCTQNLTVEKNDMSLRQLRKMFKEQLQLSKKKMDNNNNINT----- 569

Query: 721 GGKVRTALQPVPENIMTINELERRF 743
             KVRTALQP+PEN M ++ LE+ F
Sbjct: 721 --KVRTALQPLPENRMAMDGLEKLF 569

BLAST of Bhi12G000502 vs. ExPASy TrEMBL
Match: A0A6J1ESK4 (uncharacterized protein LOC111435613 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111435613 PE=4 SV=1)

HSP 1 Score: 606.3 bits (1562), Expect = 1.7e-169
Identity = 418/746 (56.03%), Postives = 478/746 (64.08%), Query Frame = 0

Query: 1   MDFRTLSRKELQALCKRNKIPANITNVAMADALAALSSVEGIEEFFNGERSGVPESPMKP 60
           MDF +LSR+ELQ LCKRNKIPANITNVAMADAL+AL  VEGI+EF + E SGVPESPMK 
Sbjct: 1   MDFHSLSRRELQTLCKRNKIPANITNVAMADALSALPLVEGIDEFLSSEGSGVPESPMKG 60

Query: 61  EVISSEIPRTALRTSTRRKAVKDEAITTRTRRAAAARDTEESENRDLNVALTTPSLPGSR 120
           EV+SSEIPRT LRTSTRRK VKD+ ITTRTRRAAAAR TEESENRDLNV LTTPSL  SR
Sbjct: 61  EVVSSEIPRTELRTSTRRKTVKDDPITTRTRRAAAARGTEESENRDLNVTLTTPSLRSSR 120

Query: 121 RRTAAASSACKKVDFQMTVDDQKEDNDLDKKKKAIEKTPAVPKS-LRVAGASTRKRTETR 180
           R TA ASSACKKVDFQMTVD++ EDNDLD +K  IE TPAVPKS  RVAGAST KRT+TR
Sbjct: 121 RITAGASSACKKVDFQMTVDEE-EDNDLDHEKPVIENTPAVPKSQKRVAGASTSKRTQTR 180

Query: 181 NNGAAEQRVYSTRRSVRFLEKNMESLSLGEDREMESITVHMSFDDMPNSS-VGAGPVKED 240
           NNGA E RVYSTRRSVR LE+ MESLSL ED++ME  TVHMSFD+MPNSS +GAGPVKE+
Sbjct: 181 NNGAMEPRVYSTRRSVRLLEQTMESLSLAEDKKMEPNTVHMSFDEMPNSSAIGAGPVKEE 240

Query: 241 TELETESKKSDESESKLDKDTGVEIDDQEKNEMESEVELSEEEQEMVLDDPLKSSEEKPA 300
           T+LE ESK +D+SESKLD+D G++ ++QEKNEM        EE EM  DD +K S +   
Sbjct: 241 TKLEVESKNTDKSESKLDEDLGLKTNEQEKNEM--------EEPEMDPDDSMKRSAD--- 300

Query: 301 TANNGDDTGTEAPHANSDVKCFSEDEEANEASKDDVSVEISAEEINDSDKLSQPTEDGDL 360
                                               SVEISAEEIND DKLS  +ED DL
Sbjct: 301 ------------------------------------SVEISAEEINDFDKLSLHSEDVDL 360

Query: 361 TEVVDNSIIVEESGMEVESKQNESTCELKHIIEEVINEEVKLMNDTAEKLSLPHENVEEE 420
           TE V +S I EESGMEV+SKQNES  EL+H            MN +AE+ SLP ENVEEE
Sbjct: 361 TE-VHHSSIEEESGMEVQSKQNESIFELEH------------MNISAEESSLPIENVEEE 420

Query: 421 AQVDDDVLSSDEESVMEVKDDQTQVEAENESTYELEHIIEKVNEEEVKLMKDTAEELSLP 480
            QVD+                                                       
Sbjct: 421 TQVDE------------------------------------------------------- 480

Query: 481 HESVEEKTQVDDGVLSSDEESVIEVKDDQDEYDYESDPTIEEEASDADIEVDKKETEMEK 540
                        VLSS E+S++EV DD+DE     +PTIEEE                 
Sbjct: 481 -------------VLSSVEKSIMEVIDDEDEL----EPTIEEE----------------- 540

Query: 541 VSVQAEEDDTTINTSMEEFPENVSEDLVDVKIQWQMEEEAPSKDIDVDAIDSQPKIPNDN 600
                         S+ EFPENVSEDLVDVKIQ+QME+  P+ D +VD  DS P IPND 
Sbjct: 541 --------------SVMEFPENVSEDLVDVKIQYQMEKSPPT-DTNVDTTDSHPDIPNDI 565

Query: 601 VQCEEALVEIIPTSSNNNGVQSLPSFGADQLALATQFPRPT-VGTKSPVREQTIRLLMDN 660
           +QCEEAL+E +  SSNNNG+QSLPS  A QLALA QFPRP+ V TKS  +EQ  RLLMDN
Sbjct: 601 LQCEEALIE-VSVSSNNNGIQSLPSLAASQLALANQFPRPSVVETKSSHKEQATRLLMDN 565

Query: 661 SDAEEEEDGKEQEKANCIEKQIVETNDMSLRQLKKMFKEKLQLSKQKMD-NNSINSKVVG 720
           SDAEEE        ANC +KQ VE NDMSLRQL+KMFKE+LQLSK+KMD NN+IN+    
Sbjct: 661 SDAEEE--------ANCTQKQTVEKNDMSLRQLRKMFKEQLQLSKKKMDNNNNINT---- 565

Query: 721 GGGKVRTALQPVPENIMTINELERRF 743
              KVRTALQP+PEN M ++ LE+ F
Sbjct: 721 ---KVRTALQPLPENRMAMDGLEKLF 565