Bhi12G000444 (gene) Wax gourd (B227) v1

Overview
NameBhi12G000444
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionProcollagen-proline 4-dioxygenase
Locationchr12: 15702685 .. 15705819 (-)
RNA-Seq ExpressionBhi12G000444
SyntenyBhi12G000444
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATACAATCTCGGTCCCCGATCTCGTTTGAGAATGCCCGAGAAAAACAAATTCTATGATCTTTGTATTTTTTAAATGTAAAAATTATTTTGGAGAATTTCCTAAGAATATCTTTAATCTGTCGCAAACGCGAGAGAATGGAACAGATTCATAGAACTACAGAACACACCATGGATTTCTACACCACCAGCTTTCTGAAAATCTGTATCCACGTTCTCTAATTTCGTCTTCAACTTCGTCTTGTTTCGATCTTCCTCCCACCCATCCATGGATTCTCGTCTTAACTTTTTGCTTCTTTTAGCGACTGCATTTTCATTCTCAACCTGCCTTGCACAAAGGTGATAACCCAACCCCATTTTACATCTCATTTCGAAATCTTAGGTTTTACATATTTCGTTCTTCGCATCTTATGTTTGCTGCGTAAATTGGATTCTCATCAATTTCATCTTCGGATGCTCAATCGGAATTGTGTTTAGAATGATTTTTTGATGCATGATTGTGTTTGATATCATGGGTTTTTCTTCTTTCCCTTGAATTTGTTTTATTCATTCTGCCAGCAATTTGATTAGTGGCCGGAAGGGTTTAAGAGATCAATTGGTTGATAGACCTTTGAGCTACTCAAATCATTCAGGAAGAATCGACCCATCAAGAGTTGTCCAAGTCTCTTGGCAACCAAGGTCAGGTACTTGCAGAATCTCTCATATGATTTGGAATTATTTCATCAACTTCAAAACATATTGATTAACTTAGTAGAAAGTCTGATTTAACTTTTCTTCCTGTAGGGTTTTCTTGTATAAAGGTTTTCTCTCAGATGAGGAGTGTGATCATCTGATTTCTTTGGTATTTTTCTGCCCTGTTCTTGTTAGTATCATCAACTTCCTTCAGCTGTTTCTGACGTCCGTATAACTTTTGGGATTAGGCTTCAAATTCAGAAGACAATCCTTCTGGGAACAGTGCTGGTTCCGGGAACACTGTCTCCACCAAATTGCTAAACAGTTCAGGAGTCATTTTAAACACATCAGTATGTTCCTGTGCTTTTTGAAGTTTATGCAATGGTTTGAATAGTACACTTCGTGGTCAAATTTGTATTTCGTAGCACGAGATTTGTTTATCTTGTTTTCATGAAGTTTAAGTTGAAGTAGTATGTTTCTCAATTATATATTGTTTTCTGTTAGTTTCTACACGTGAGCAATTATGCAGGCTATCAGTTATAGAACTTTTTCTGTCATCATTGTTTTCTTTATTTCCAGTATTTTTATTCAAAATATAACTACAAATTGTACATGGATAAACAGTTTCATCTTTGCTTGGCAATTGTTTGAATTAAACTATCCGTTCAACGTATTTTCCATTGACATATGTTCAGTCTAAAGTTTTCTGTTGATAGTATTCACAATGGTGCTACCAAATATTCGTTTTGCATATTAGGATCTGGTACTTGGTATACCTGCTTTTAATCTATAATTATTGGGAGGTGTGGGGTTGTTTTTCCAAAGGTTCCTTCTCTACTTAAATTTTCTTGATGTGCCACGTTTAGGATTGATGACATTTCATACGCTGTCAACCCATCCCAATACCCATAGAAGTATGCTTTGTTGCTGTATTTTCTGAGGGCTCAGTAGTAGTTTTAAAGGAATCTAAAATAACTCTCAACTCTAGATGTCCTGTGAAGTCAAACGTTCATTTCTGCATTTGTAGATAAGAACACTAAAAATGTGGAGGACGGTAGCTTAACATAACCTATGATAGCTCATGCAAAATTCCATAATTATTACTAAAATAATTATTTTTGGTGATTCAATTTTGAGTTGTCAGTTGTCTTTCAATGTTTATAACGAACCATGGCTCAAGGGAGTAATTGACCGACCCAAGAATTTAGTATTGTGTTCCTTTTTTGCAATACCATCTATTTCTGTTCATGGTATTTATGTTTTGTGGAGCTTGTCAATTACTTCTCAAGTAACTAGGTCTTGTGAGATAATAAAACTCAAAGTCACTGCTGTTGGTGTATTTCTATTTTCTATGAGATTTATTCTTCGACTTCTTGTTTGGGCTTGTTTCTTAGGATGATATCATTGCAAGAATTGAAAATCAAATTGCAGTATGGACTTTTCTTCCAAAAGGTATTTCTCATCAGTGCTGTACTGCAACTGGCTTTGCCCTTTCAATTTTCCTTATAATGCTCCTTTTGTTTGATTTTCTTCTAAACACGTTAGTTTCCTTCTCTTCAGATCATGGCATGCCTTTTCAGATCATGCAATATAGGGGTGAAGAAGCAGAACATAAGTACTTTTATGGCAACGGATCTGCAATGTCGTCCAGTGAGCCTTTGATGGCCACAGTAGTTTTGTATCTCTCAGATTCTGCTCGCGGTGGCGAGATGCTCTTTCCAGAATCAAAGGTGAGGGGAAGTACTCAAAGATTTGTGGCCATAACAATGTACTCATGACTGCCTTCTTCTCTATGCCTCAGGTAAAGAGCAAGTTTTGGTCAGACCGTAGAAAGAAAAACAACTTTCTGAGACCAGTGAAAGGCAATGCAATTCTTTTTTTCTCTGTGCATCTAAATGCTTCTCCAGACAAGAGCAGCTACCATACTCGATCTCCAATACTCAATGGGGAATTATGGGTTGCTACAAAATTCTTCTACTTAAGACCAACCACTGGGAACAAACGCACAGTTGAATCCGATGTAGACGGGTGCATTGATGAAGATAAAAGCTGCCCTCAATGGGCTGCCATTGGCGAATGCGAACGAAACACTGTGTTCATGATCGGTTCTCCAGATTACTATGGTACATGTAGAAAAAGCTGCAATGCATGCTGATACATAACCAAATTCAAGTATCGTCCTGATTTGAGCAAGTATTTGTTTATTTTTTCTGATCTCATACAAGTACCACTCCGAATGAGTGTATTTTCTTCCTTTTTTGGGGTTATTGTTTGGATATTGGATTGTATTGGCATATCTCTTGGTTAGTAGTTCTTGCACCTTAAGAACTATATAGGATTAGGAATTCTAGTAACATCTCATAAATATAATATTAAACCACATCTTCCGTATGTCTTGGGATTATTACACTTCTGCCTTTCATTTTAGTTTATGTAATCTTAGAATGTTTATTTTGATTTGCTTCA

mRNA sequence

ATACAATCTCGGTCCCCGATCTCGTTTGAGAATGCCCGAGAAAAACAAATTCTATGATCTTTGTATTTTTTAAATGTAAAAATTATTTTGGAGAATTTCCTAAGAATATCTTTAATCTGTCGCAAACGCGAGAGAATGGAACAGATTCATAGAACTACAGAACACACCATGGATTTCTACACCACCAGCTTTCTGAAAATCTGTATCCACGTTCTCTAATTTCGTCTTCAACTTCGTCTTGTTTCGATCTTCCTCCCACCCATCCATGGATTCTCGTCTTAACTTTTTGCTTCTTTTAGCGACTGCATTTTCATTCTCAACCTGCCTTGCACAAAGCAATTTGATTAGTGGCCGGAAGGGTTTAAGAGATCAATTGGTTGATAGACCTTTGAGCTACTCAAATCATTCAGGAAGAATCGACCCATCAAGAGTTGTCCAAGTCTCTTGGCAACCAAGGGTTTTCTTGTATAAAGGTTTTCTCTCAGATGAGGAGTGTGATCATCTGATTTCTTTGGCTTCAAATTCAGAAGACAATCCTTCTGGGAACAGTGCTGGTTCCGGGAACACTGTCTCCACCAAATTGCTAAACAGTTCAGGAGTCATTTTAAACACATCAGATGATATCATTGCAAGAATTGAAAATCAAATTGCAGTATGGACTTTTCTTCCAAAAGATCATGGCATGCCTTTTCAGATCATGCAATATAGGGGTGAAGAAGCAGAACATAAGTACTTTTATGGCAACGGATCTGCAATGTCGTCCAGTGAGCCTTTGATGGCCACAGTAGTTTTGTATCTCTCAGATTCTGCTCGCGGTGGCGAGATGCTCTTTCCAGAATCAAAGGTAAAGAGCAAGTTTTGGTCAGACCGTAGAAAGAAAAACAACTTTCTGAGACCAGTGAAAGGCAATGCAATTCTTTTTTTCTCTGTGCATCTAAATGCTTCTCCAGACAAGAGCAGCTACCATACTCGATCTCCAATACTCAATGGGGAATTATGGGTTGCTACAAAATTCTTCTACTTAAGACCAACCACTGGGAACAAACGCACAGTTGAATCCGATGTAGACGGGTGCATTGATGAAGATAAAAGCTGCCCTCAATGGGCTGCCATTGGCGAATGCGAACGAAACACTGTGTTCATGATCGGTTCTCCAGATTACTATGGTACATGTAGAAAAAGCTGCAATGCATGCTGATACATAACCAAATTCAAGTATCGTCCTGATTTGAGCAAGTATTTGTTTATTTTTTCTGATCTCATACAAGTACCACTCCGAATGAGTGTATTTTCTTCCTTTTTTGGGGTTATTGTTTGGATATTGGATTGTATTGGCATATCTCTTGGTTAGTAGTTCTTGCACCTTAAGAACTATATAGGATTAGGAATTCTAGTAACATCTCATAAATATAATATTAAACCACATCTTCCGTATGTCTTGGGATTATTACACTTCTGCCTTTCATTTTAGTTTATGTAATCTTAGAATGTTTATTTTGATTTGCTTCA

Coding sequence (CDS)

ATGGATTCTCGTCTTAACTTTTTGCTTCTTTTAGCGACTGCATTTTCATTCTCAACCTGCCTTGCACAAAGCAATTTGATTAGTGGCCGGAAGGGTTTAAGAGATCAATTGGTTGATAGACCTTTGAGCTACTCAAATCATTCAGGAAGAATCGACCCATCAAGAGTTGTCCAAGTCTCTTGGCAACCAAGGGTTTTCTTGTATAAAGGTTTTCTCTCAGATGAGGAGTGTGATCATCTGATTTCTTTGGCTTCAAATTCAGAAGACAATCCTTCTGGGAACAGTGCTGGTTCCGGGAACACTGTCTCCACCAAATTGCTAAACAGTTCAGGAGTCATTTTAAACACATCAGATGATATCATTGCAAGAATTGAAAATCAAATTGCAGTATGGACTTTTCTTCCAAAAGATCATGGCATGCCTTTTCAGATCATGCAATATAGGGGTGAAGAAGCAGAACATAAGTACTTTTATGGCAACGGATCTGCAATGTCGTCCAGTGAGCCTTTGATGGCCACAGTAGTTTTGTATCTCTCAGATTCTGCTCGCGGTGGCGAGATGCTCTTTCCAGAATCAAAGGTAAAGAGCAAGTTTTGGTCAGACCGTAGAAAGAAAAACAACTTTCTGAGACCAGTGAAAGGCAATGCAATTCTTTTTTTCTCTGTGCATCTAAATGCTTCTCCAGACAAGAGCAGCTACCATACTCGATCTCCAATACTCAATGGGGAATTATGGGTTGCTACAAAATTCTTCTACTTAAGACCAACCACTGGGAACAAACGCACAGTTGAATCCGATGTAGACGGGTGCATTGATGAAGATAAAAGCTGCCCTCAATGGGCTGCCATTGGCGAATGCGAACGAAACACTGTGTTCATGATCGGTTCTCCAGATTACTATGGTACATGTAGAAAAAGCTGCAATGCATGCTGA

Protein sequence

MDSRLNFLLLLATAFSFSTCLAQSNLISGRKGLRDQLVDRPLSYSNHSGRIDPSRVVQVSWQPRVFLYKGFLSDEECDHLISLASNSEDNPSGNSAGSGNTVSTKLLNSSGVILNTSDDIIARIENQIAVWTFLPKDHGMPFQIMQYRGEEAEHKYFYGNGSAMSSSEPLMATVVLYLSDSARGGEMLFPESKVKSKFWSDRRKKNNFLRPVKGNAILFFSVHLNASPDKSSYHTRSPILNGELWVATKFFYLRPTTGNKRTVESDVDGCIDEDKSCPQWAAIGECERNTVFMIGSPDYYGTCRKSCNAC
Homology
[Close]
BLAST of Bhi12G000444 vs. TAIR 10
Match: AT4G25600.1 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 245.0 bits (624), Expect = 8.1e-65
Identity = 139/310 (44.84%), Postives = 190/310 (61.29%), Query Frame = 0 

Query: 7   FLLLLATAFSFSTCLAQSNLISGRKGLRDQLV-----DRPLSYSNHSGRIDPSRVVQVSW 66
           FL+L+ T  S S           RK LRD+ +     D   SY   S  +DP+RV+Q+SW
Sbjct: 8   FLILMITMSSSSPPFCSG---GSRKELRDKEITSKSDDTQASYVLGSKFVDPTRVLQLSW 67

Query: 67  QPRVFLYKGFLSDEECDHLISLASNSEDNPSGNSAGSGNTVSTKLLNSSGVILNTSDDII 126
            PRVFLY+GFLS+EECDHLISL   + +  S ++ G      T+L           D ++
Sbjct: 68  LPRVFLYRGFLSEEECDHLISLRKETTEVYSVDADG-----KTQL-----------DPVV 127

Query: 127 ARIENQIAVWTFLPKDHGMPFQIMQYRGEEAEHKY-FYGNGSAMSSSEPLMATVVLYLSD 186
           A IE +++ WTFLP ++G   ++  Y  E++  K  ++G   +    E L+ATVVLYLS+
Sbjct: 128 AGIEEKVSAWTFLPGENGGSIKVRSYTSEKSGKKLDYFGEEPSSVLHESLLATVVLYLSN 187

Query: 187 SARGGEMLFPESKVKSKFWSDRRKKNNFLRPVKGNAILFFSVHLNASPDKSSYHTRSPIL 246
           + +GGE+LFP S++K K  +   +  N LRPVKGNAILFF+  LNAS D  S H R P++
Sbjct: 188 TTQGGELLFPNSEMKPK--NSCLEGGNILRPVKGNAILFFTRLLNASLDGKSTHLRCPVV 247

Query: 247 NGELWVATKFFYLRPTTGNKRTVESDVDGCIDEDKSCPQWAAIGECERNTVFMIGSPDYY 306
            GEL VATK  Y +     K+    +   C DED++C +WA +GEC++N V+MIGSPDYY
Sbjct: 248 KGELLVATKLIYAK-----KQARIEESGECSDEDENCGRWAKLGECKKNPVYMIGSPDYY 291

Query: 307 GTCRKSCNAC 311
           GTCRKSCNAC
Sbjct: 308 GTCRKSCNAC 291

[Close]
BLAST of Bhi12G000444 vs. TAIR 10
Match: AT3G28490.1 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 220.3 bits (560), Expect = 2.1e-57
Identity = 115/276 (41.67%), Postives = 169/276 (61.23%), Query Frame = 0 

Query: 45  SNHSGRIDPSRVVQVSWQPRVFLYKGFLSDEECDHLISLASNS-EDNPSGNSAGSGNTVS 104
           S+ S  +DP+R+ Q+SW PR FLYKGFLSDEECDHLI LA    E +       SG +  
Sbjct: 21  SSFSFSVDPTRITQLSWTPRAFLYKGFLSDEECDHLIKLAKGKLEKSMVVADVDSGESED 80

Query: 105 TKLLNSSGVIL-NTSDDIIARIENQIAVWTFLPKDHGMPFQIMQYRG---EEAEHKYFYG 164
           +++  SSG+ L    DDI+A +E ++A WTFLP+++G   QI+ Y      +    YFY 
Sbjct: 81  SEVRTSSGMFLTKRQDDIVANVEAKLAAWTFLPEENGEALQILHYENGQKYDPHFDYFY- 140

Query: 165 NGSAMSSSEPLMATVVLYLSDSARGGEMLFPESK-----VKSKFWSDRRKKNNFLRPVKG 224
           +  A+      +ATV++YLS+  +GGE +FP  K     +K   WS   K+   ++P KG
Sbjct: 141 DKKALELGGHRIATVLMYLSNVTKGGETVFPNWKGKTPQLKDDSWSKCAKQGYAVKPRKG 200

Query: 225 NAILFFSVHLNASPDKSSYHTRSPILNGELWVATKFFYLRPTTGNKRTVESDVDGCIDED 284
           +A+LFF++HLN + D +S H   P++ GE W AT++ ++R + G K+ V      C+D+ 
Sbjct: 201 DALLFFNLHLNGTTDPNSLHGSCPVIEGEKWSATRWIHVR-SFGKKKLV------CVDDH 260

Query: 285 KSCPQWAAIGECERNTVFMIGSPDYYGTCRKSCNAC 311
           +SC +WA  GECE+N ++M+GS    G CRKSC AC
Sbjct: 261 ESCQEWADAGECEKNPMYMVGSETSLGFCRKSCKAC 288

[Close]
BLAST of Bhi12G000444 vs. TAIR 10
Match: AT3G28480.1 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 218.0 bits (554), Expect = 1.1e-56
Identity = 123/323 (38.08%), Postives = 192/323 (59.44%), Query Frame = 0 

Query: 1   MDSRLNFLLLLATAFSFSTCL---AQSNLISGRKGLRDQLVDRPLSYSNHSGRIDPSRVV 60
           MDSR+   L  +  F F+  L   A +  ++     RD  V + +  S  S   DP+RV 
Sbjct: 1   MDSRI--FLAFSLCFLFTLPLISSAPNRFLTRSSNTRDGSVIK-MKTSASSFGFDPTRVT 60

Query: 61  QVSWQPRVFLYKGFLSDEECDHLISLASNSEDNPSGNSAGSGNTVSTKLLNSSGVILN-T 120
           Q+SW PRVFLY+GFLSDEECDH I LA    +        SG +V +++  SSG+ L+  
Sbjct: 61  QLSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEVRTSSGMFLSKR 120

Query: 121 SDDIIARIENQIAVWTFLPKDHGMPFQIMQY-RGEEAEHKYFYGNGSA-MSSSEPLMATV 180
            DDI++ +E ++A WTFLP+++G   QI+ Y  G++ E  + Y +  A +      +ATV
Sbjct: 121 QDDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQANLELGGHRIATV 180

Query: 181 VLYLSDSARGGEMLFP-----ESKVKSKFWSDRRKKNNFLRPVKGNAILFFSVHLNASPD 240
           ++YLS+  +GGE +FP      +++K   W++  K+   ++P KG+A+LFF++H NA+ D
Sbjct: 181 LMYLSNVEKGGETVFPMWKGKATQLKDDSWTECAKQGYAVKPRKGDALLFFNLHPNATTD 240

Query: 241 KSSYHTRSPILNGELWVATKFFYLR--PTTGNKRTVESDVDGCIDEDKSCPQWAAIGECE 300
            +S H   P++ GE W AT++ +++      NK++      GC+DE+ SC +WA  GEC+
Sbjct: 241 SNSLHGSCPVVEGEKWSATRWIHVKSFERAFNKQS------GCMDENVSCEKWAKAGECQ 300

Query: 301 RNTVFMIGSPDYYGTCRKSCNAC 311
           +N  +M+GS   +G CRKSC AC
Sbjct: 301 KNPTYMVGSDKDHGYCRKSCKAC 314

[Close]
BLAST of Bhi12G000444 vs. TAIR 10
Match: AT3G28480.2 (Oxoglutarate/iron-dependent oxygenase )

HSP 1 Score: 214.9 bits (546), Expect = 9.0e-56
Identity = 129/332 (38.86%), Postives = 194/332 (58.43%), Query Frame = 0 

Query: 1   MDSRLNFLLLLATAFSFSTCL---AQSNLISGRKGLRDQLVDRPLSYSNHSGRIDPSRVV 60
           MDSR+   L  +  F F+  L   A +  ++     RD  V + +  S  S   DP+RV 
Sbjct: 1   MDSRI--FLAFSLCFLFTLPLISSAPNRFLTRSSNTRDGSVIK-MKTSASSFGFDPTRVT 60

Query: 61  QVSWQPRVFLYKGFLSDEECDHLISLA------SNSEDNPSGNSAGSGNTVSTKLLNSSG 120
           Q+SW PRVFLY+GFLSDEECDH I LA      S   DN SG S  S ++VS  +  SS 
Sbjct: 61  QLSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEDSVSV-VRQSSS 120

Query: 121 VILNTS----DDIIARIENQIAVWTFLPKDHGMPFQIMQY-RGEEAEHKYFYGNGSA-MS 180
            I N      DDI++ +E ++A WTFLP+++G   QI+ Y  G++ E  + Y +  A + 
Sbjct: 121 FIANMDSLEIDDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQANLE 180

Query: 181 SSEPLMATVVLYLSDSARGGEMLFP-----ESKVKSKFWSDRRKKNNFLRPVKGNAILFF 240
                +ATV++YLS+  +GGE +FP      +++K   W++  K+   ++P KG+A+LFF
Sbjct: 181 LGGHRIATVLMYLSNVEKGGETVFPMWKGKATQLKDDSWTECAKQGYAVKPRKGDALLFF 240

Query: 241 SVHLNASPDKSSYHTRSPILNGELWVATKFFYLR--PTTGNKRTVESDVDGCIDEDKSCP 300
           ++H NA+ D +S H   P++ GE W AT++ +++      NK++      GC+DE+ SC 
Sbjct: 241 NLHPNATTDSNSLHGSCPVVEGEKWSATRWIHVKSFERAFNKQS------GCMDENVSCE 300

Query: 301 QWAAIGECERNTVFMIGSPDYYGTCRKSCNAC 311
           +WA  GEC++N  +M+GS   +G CRKSC AC
Sbjct: 301 KWAKAGECQKNPTYMVGSDKDHGYCRKSCKAC 322

[Close]
BLAST of Bhi12G000444 vs. TAIR 10
Match: AT3G06300.1 (P4H isoform 2 )

HSP 1 Score: 191.0 bits (484), Expect = 1.4e-48
Identity = 103/278 (37.05%), Postives = 166/278 (59.71%), Query Frame = 0 

Query: 45  SNHSGRIDPSRVVQVSWQPRVFLYKGFLSDEECDHLISLASNSEDNPSGNSAGSGNTVST 104
           S+ S  I+PS+V QVS +PR F+Y+GFL+D ECDHLISLA  +    +     +G +  +
Sbjct: 27  SSPSSIINPSKVKQVSSKPRAFVYEGFLTDLECDHLISLAKENLQRSAVADNDNGESQVS 86

Query: 105 KLLNSSGVILNT-SDDIIARIENQIAVWTFLPKDHGMPFQIMQY---RGEEAEHKYFYGN 164
            +  SSG  ++   D I++ IE++++ WTFLPK++G   Q+++Y   +  +A   YF+  
Sbjct: 87  DVRTSSGTFISKGKDPIVSGIEDKLSTWTFLPKENGEDLQVLRYEHGQKYDAHFDYFHDK 146

Query: 165 GSAMSSSEPLMATVVLYLSDSARGGEMLFPESKVKSK--------FWSDRRKKNNFLRPV 224
            +       + ATV+LYLS+  +GGE +FP+++  S+          SD  KK   ++P 
Sbjct: 147 VNIARGGHRI-ATVLLYLSNVTKGGETVFPDAQEFSRRSLSENKDDLSDCAKKGIAVKPK 206

Query: 225 KGNAILFFSVHLNASPDKSSYHTRSPILNGELWVATKFFYLRPTTGNKRTVESDVDGCID 284
           KGNA+LFF++  +A PD  S H   P++ GE W ATK+ ++     +   + +    C D
Sbjct: 207 KGNALLFFNLQQDAIPDPFSLHGGCPVIEGEKWSATKWIHV----DSFDKILTHDGNCTD 266

Query: 285 EDKSCPQWAAIGECERNTVFMIGSPDYYGTCRKSCNAC 311
            ++SC +WA +GEC +N  +M+G+P+  G CR+SC AC
Sbjct: 267 VNESCERWAVLGECGKNPEYMVGTPEIPGNCRRSCKAC 299

[Close]
BLAST of Bhi12G000444 vs. ExPASy Swiss-Prot
Match: Q8GXT7 (Probable prolyl 4-hydroxylase 12 OS=Arabidopsis thaliana OX=3702 GN=P4H12 PE=2 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 1.1e-63
Identity = 139/310 (44.84%), Postives = 190/310 (61.29%), Query Frame = 0 

Query: 7   FLLLLATAFSFSTCLAQSNLISGRKGLRDQLV-----DRPLSYSNHSGRIDPSRVVQVSW 66
           FL+L+ T  S S           RK LRD+ +     D   SY   S  +DP+RV+Q+SW
Sbjct: 8   FLILMITMSSSSPPFCSG---GSRKELRDKEITSKSDDTQASYVLGSKFVDPTRVLQLSW 67

Query: 67  QPRVFLYKGFLSDEECDHLISLASNSEDNPSGNSAGSGNTVSTKLLNSSGVILNTSDDII 126
            PRVFLY+GFLS+EECDHLISL   + +  S ++ G      T+L           D ++
Sbjct: 68  LPRVFLYRGFLSEEECDHLISLRKETTEVYSVDADG-----KTQL-----------DPVV 127

Query: 127 ARIENQIAVWTFLPKDHGMPFQIMQYRGEEAEHKY-FYGNGSAMSSSEPLMATVVLYLSD 186
           A IE +++ WTFLP ++G   ++  Y  E++  K  ++G   +    E L+ATVVLYLS+
Sbjct: 128 AGIEEKVSAWTFLPGENGGSIKVRSYTSEKSGKKLDYFGEEPSSVLHESLLATVVLYLSN 187

Query: 187 SARGGEMLFPESKVKSKFWSDRRKKNNFLRPVKGNAILFFSVHLNASPDKSSYHTRSPIL 246
           + +GGE+LFP S++K K  +   +  N LRPVKGNAILFF+  LNAS D  S H R P++
Sbjct: 188 TTQGGELLFPNSEMKPK--NSCLEGGNILRPVKGNAILFFTRLLNASLDGKSTHLRCPVV 247

Query: 247 NGELWVATKFFYLRPTTGNKRTVESDVDGCIDEDKSCPQWAAIGECERNTVFMIGSPDYY 306
            GEL VATK  Y +     K+    +   C DED++C +WA +GEC++N V+MIGSPDYY
Sbjct: 248 KGELLVATKLIYAK-----KQARIEESGECSDEDENCGRWAKLGECKKNPVYMIGSPDYY 291

Query: 307 GTCRKSCNAC 311
           GTCRKSCNAC
Sbjct: 308 GTCRKSCNAC 291

[Close]
BLAST of Bhi12G000444 vs. ExPASy Swiss-Prot
Match: F4J0A8 (Probable prolyl 4-hydroxylase 6 OS=Arabidopsis thaliana OX=3702 GN=P4H6 PE=2 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 3.0e-56
Identity = 115/276 (41.67%), Postives = 169/276 (61.23%), Query Frame = 0 

Query: 45  SNHSGRIDPSRVVQVSWQPRVFLYKGFLSDEECDHLISLASNS-EDNPSGNSAGSGNTVS 104
           S+ S  +DP+R+ Q+SW PR FLYKGFLSDEECDHLI LA    E +       SG +  
Sbjct: 21  SSFSFSVDPTRITQLSWTPRAFLYKGFLSDEECDHLIKLAKGKLEKSMVVADVDSGESED 80

Query: 105 TKLLNSSGVIL-NTSDDIIARIENQIAVWTFLPKDHGMPFQIMQYRG---EEAEHKYFYG 164
           +++  SSG+ L    DDI+A +E ++A WTFLP+++G   QI+ Y      +    YFY 
Sbjct: 81  SEVRTSSGMFLTKRQDDIVANVEAKLAAWTFLPEENGEALQILHYENGQKYDPHFDYFY- 140

Query: 165 NGSAMSSSEPLMATVVLYLSDSARGGEMLFPESK-----VKSKFWSDRRKKNNFLRPVKG 224
           +  A+      +ATV++YLS+  +GGE +FP  K     +K   WS   K+   ++P KG
Sbjct: 141 DKKALELGGHRIATVLMYLSNVTKGGETVFPNWKGKTPQLKDDSWSKCAKQGYAVKPRKG 200

Query: 225 NAILFFSVHLNASPDKSSYHTRSPILNGELWVATKFFYLRPTTGNKRTVESDVDGCIDED 284
           +A+LFF++HLN + D +S H   P++ GE W AT++ ++R + G K+ V      C+D+ 
Sbjct: 201 DALLFFNLHLNGTTDPNSLHGSCPVIEGEKWSATRWIHVR-SFGKKKLV------CVDDH 260

Query: 285 KSCPQWAAIGECERNTVFMIGSPDYYGTCRKSCNAC 311
           +SC +WA  GECE+N ++M+GS    G CRKSC AC
Sbjct: 261 ESCQEWADAGECEKNPMYMVGSETSLGFCRKSCKAC 288

[Close]
BLAST of Bhi12G000444 vs. ExPASy Swiss-Prot
Match: Q8L970 (Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 1.5e-55
Identity = 123/323 (38.08%), Postives = 192/323 (59.44%), Query Frame = 0 

Query: 1   MDSRLNFLLLLATAFSFSTCL---AQSNLISGRKGLRDQLVDRPLSYSNHSGRIDPSRVV 60
           MDSR+   L  +  F F+  L   A +  ++     RD  V + +  S  S   DP+RV 
Sbjct: 1   MDSRI--FLAFSLCFLFTLPLISSAPNRFLTRSSNTRDGSVIK-MKTSASSFGFDPTRVT 60

Query: 61  QVSWQPRVFLYKGFLSDEECDHLISLASNSEDNPSGNSAGSGNTVSTKLLNSSGVILN-T 120
           Q+SW PRVFLY+GFLSDEECDH I LA    +        SG +V +++  SSG+ L+  
Sbjct: 61  QLSWTPRVFLYEGFLSDEECDHFIKLAKGKLEKSMVADNDSGESVESEVRTSSGMFLSKR 120

Query: 121 SDDIIARIENQIAVWTFLPKDHGMPFQIMQY-RGEEAEHKYFYGNGSA-MSSSEPLMATV 180
            DDI++ +E ++A WTFLP+++G   QI+ Y  G++ E  + Y +  A +      +ATV
Sbjct: 121 QDDIVSNVEAKLAAWTFLPEENGESMQILHYENGQKYEPHFDYFHDQANLELGGHRIATV 180

Query: 181 VLYLSDSARGGEMLFP-----ESKVKSKFWSDRRKKNNFLRPVKGNAILFFSVHLNASPD 240
           ++YLS+  +GGE +FP      +++K   W++  K+   ++P KG+A+LFF++H NA+ D
Sbjct: 181 LMYLSNVEKGGETVFPMWKGKATQLKDDSWTECAKQGYAVKPRKGDALLFFNLHPNATTD 240

Query: 241 KSSYHTRSPILNGELWVATKFFYLR--PTTGNKRTVESDVDGCIDEDKSCPQWAAIGECE 300
            +S H   P++ GE W AT++ +++      NK++      GC+DE+ SC +WA  GEC+
Sbjct: 241 SNSLHGSCPVVEGEKWSATRWIHVKSFERAFNKQS------GCMDENVSCEKWAKAGECQ 300

Query: 301 RNTVFMIGSPDYYGTCRKSCNAC 311
           +N  +M+GS   +G CRKSC AC
Sbjct: 301 KNPTYMVGSDKDHGYCRKSCKAC 314

[Close]
BLAST of Bhi12G000444 vs. ExPASy Swiss-Prot
Match: F4JAU3 (Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=P4H2 PE=1 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 2.0e-47
Identity = 103/278 (37.05%), Postives = 166/278 (59.71%), Query Frame = 0 

Query: 45  SNHSGRIDPSRVVQVSWQPRVFLYKGFLSDEECDHLISLASNSEDNPSGNSAGSGNTVST 104
           S+ S  I+PS+V QVS +PR F+Y+GFL+D ECDHLISLA  +    +     +G +  +
Sbjct: 27  SSPSSIINPSKVKQVSSKPRAFVYEGFLTDLECDHLISLAKENLQRSAVADNDNGESQVS 86

Query: 105 KLLNSSGVILNT-SDDIIARIENQIAVWTFLPKDHGMPFQIMQY---RGEEAEHKYFYGN 164
            +  SSG  ++   D I++ IE++++ WTFLPK++G   Q+++Y   +  +A   YF+  
Sbjct: 87  DVRTSSGTFISKGKDPIVSGIEDKLSTWTFLPKENGEDLQVLRYEHGQKYDAHFDYFHDK 146

Query: 165 GSAMSSSEPLMATVVLYLSDSARGGEMLFPESKVKSK--------FWSDRRKKNNFLRPV 224
            +       + ATV+LYLS+  +GGE +FP+++  S+          SD  KK   ++P 
Sbjct: 147 VNIARGGHRI-ATVLLYLSNVTKGGETVFPDAQEFSRRSLSENKDDLSDCAKKGIAVKPK 206

Query: 225 KGNAILFFSVHLNASPDKSSYHTRSPILNGELWVATKFFYLRPTTGNKRTVESDVDGCID 284
           KGNA+LFF++  +A PD  S H   P++ GE W ATK+ ++     +   + +    C D
Sbjct: 207 KGNALLFFNLQQDAIPDPFSLHGGCPVIEGEKWSATKWIHV----DSFDKILTHDGNCTD 266

Query: 285 EDKSCPQWAAIGECERNTVFMIGSPDYYGTCRKSCNAC 311
            ++SC +WA +GEC +N  +M+G+P+  G CR+SC AC
Sbjct: 267 VNESCERWAVLGECGKNPEYMVGTPEIPGNCRRSCKAC 299

[Close]
BLAST of Bhi12G000444 vs. ExPASy Swiss-Prot
Match: Q8LAN3 (Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=1)

HSP 1 Score: 189.9 bits (481), Expect = 4.3e-47
Identity = 99/278 (35.61%), Postives = 169/278 (60.79%), Query Frame = 0 

Query: 45  SNHSGRIDPSRVVQVSWQPRVFLYKGFLSDEECDHLISLASNSEDNPSGNSAGSGNTVST 104
           S+ S  ++PS+V QVS +PR F+Y+GFL++ ECDH++SLA  S    +     SG +  +
Sbjct: 26  SSSSVFVNPSKVKQVSSKPRAFVYEGFLTELECDHMVSLAKASLKRSAVADNDSGESKFS 85

Query: 105 KLLNSSGVILNT-SDDIIARIENQIAVWTFLPKDHGMPFQIMQY---RGEEAEHKYFYGN 164
           ++  SSG  ++   D I++ IE++I+ WTFLPK++G   Q+++Y   +  +A   YF+  
Sbjct: 86  EVRTSSGTFISKGKDPIVSGIEDKISTWTFLPKENGEDIQVLRYEHGQKYDAHFDYFHDK 145

Query: 165 GSAMSSSEPLMATVVLYLSDSARGGEMLFPESKVKSK--------FWSDRRKKNNFLRPV 224
            + +      MAT+++YLS+  +GGE +FP++++ S+          SD  K+   ++P 
Sbjct: 146 VNIVRGGH-RMATILMYLSNVTKGGETVFPDAEIPSRRVLSENKEDLSDCAKRGIAVKPR 205

Query: 225 KGNAILFFSVHLNASPDKSSYHTRSPILNGELWVATKFFYLRPTTGNKRTVESDVDGCID 284
           KG+A+LFF++H +A PD  S H   P++ GE W ATK+ ++     +   + +    C D
Sbjct: 206 KGDALLFFNLHPDAIPDPLSLHGGCPVIEGEKWSATKWIHV----DSFDRIVTPSGNCTD 265

Query: 285 EDKSCPQWAAIGECERNTVFMIGSPDYYGTCRKSCNAC 311
            ++SC +WA +GEC +N  +M+G+ +  G CR+SC AC
Sbjct: 266 MNESCERWAVLGECTKNPEYMVGTTELPGYCRRSCKAC 298

[Close]
BLAST of Bhi12G000444 vs. NCBI nr
Match: XP_038906497.1 (probable prolyl 4-hydroxylase 12 [Benincasa hispida])

HSP 1 Score: 635.6 bits (1638), Expect = 2.3e-178
Identity = 310/310 (100.00%), Postives = 310/310 (100.00%), Query Frame = 0 

Query: 1   MDSRLNFLLLLATAFSFSTCLAQSNLISGRKGLRDQLVDRPLSYSNHSGRIDPSRVVQVS 60
           MDSRLNFLLLLATAFSFSTCLAQSNLISGRKGLRDQLVDRPLSYSNHSGRIDPSRVVQVS
Sbjct: 1   MDSRLNFLLLLATAFSFSTCLAQSNLISGRKGLRDQLVDRPLSYSNHSGRIDPSRVVQVS 60

Query: 61  WQPRVFLYKGFLSDEECDHLISLASNSEDNPSGNSAGSGNTVSTKLLNSSGVILNTSDDI 120
           WQPRVFLYKGFLSDEECDHLISLASNSEDNPSGNSAGSGNTVSTKLLNSSGVILNTSDDI
Sbjct: 61  WQPRVFLYKGFLSDEECDHLISLASNSEDNPSGNSAGSGNTVSTKLLNSSGVILNTSDDI 120

Query: 121 IARIENQIAVWTFLPKDHGMPFQIMQYRGEEAEHKYFYGNGSAMSSSEPLMATVVLYLSD 180
           IARIENQIAVWTFLPKDHGMPFQIMQYRGEEAEHKYFYGNGSAMSSSEPLMATVVLYLSD
Sbjct: 121 IARIENQIAVWTFLPKDHGMPFQIMQYRGEEAEHKYFYGNGSAMSSSEPLMATVVLYLSD 180

Query: 181 SARGGEMLFPESKVKSKFWSDRRKKNNFLRPVKGNAILFFSVHLNASPDKSSYHTRSPIL 240
           SARGGEMLFPESKVKSKFWSDRRKKNNFLRPVKGNAILFFSVHLNASPDKSSYHTRSPIL
Sbjct: 181 SARGGEMLFPESKVKSKFWSDRRKKNNFLRPVKGNAILFFSVHLNASPDKSSYHTRSPIL 240

Query: 241 NGELWVATKFFYLRPTTGNKRTVESDVDGCIDEDKSCPQWAAIGECERNTVFMIGSPDYY 300
           NGELWVATKFFYLRPTTGNKRTVESDVDGCIDEDKSCPQWAAIGECERNTVFMIGSPDYY
Sbjct: 241 NGELWVATKFFYLRPTTGNKRTVESDVDGCIDEDKSCPQWAAIGECERNTVFMIGSPDYY 300

Query: 301 GTCRKSCNAC 311
           GTCRKSCNAC
Sbjct: 301 GTCRKSCNAC 310

[Close]
BLAST of Bhi12G000444 vs. NCBI nr
Match: XP_008436994.1 (PREDICTED: probable prolyl 4-hydroxylase 12 [Cucumis melo])

HSP 1 Score: 578.6 bits (1490), Expect = 3.3e-161
Identity = 283/311 (91.00%), Postives = 293/311 (94.21%), Query Frame = 0 

Query: 1   MDSRLNFLLLLATAFSFSTCLAQSNLISGRKGLRDQLVDRPLSYSNHSGRIDPSRVVQVS 60
           MDSRLNFLLL ATAFSFSTCLAQSNLISGRKGLRDQLVDRPLSYSN S RIDPSRVVQVS
Sbjct: 1   MDSRLNFLLLFATAFSFSTCLAQSNLISGRKGLRDQLVDRPLSYSNQSVRIDPSRVVQVS 60

Query: 61  WQPRVFLYKGFLSDEECDHLISLASNSEDNPSGNSAGSGNTVSTKLLNSSGVILNTSDDI 120
           W+PRVFLYKGFLSDEECDHLISLASNSEDNPS NSAGSGNTVST+LLN SGVILNT+DDI
Sbjct: 61  WRPRVFLYKGFLSDEECDHLISLASNSEDNPSRNSAGSGNTVSTELLNGSGVILNTTDDI 120

Query: 121 IARIENQIAVWTFLPKDHGMPFQIMQYRGEEAEHKYFYGNGSAM-SSSEPLMATVVLYLS 180
           IARIEN+IAVWT LPKDHGMPFQIMQYRGEEA+HKYFYGN SAM SSSEPLMATVVLYLS
Sbjct: 121 IARIENRIAVWTLLPKDHGMPFQIMQYRGEEAKHKYFYGNRSAMSSSSEPLMATVVLYLS 180

Query: 181 DSARGGEMLFPESKVKSKFWSDRRKKNNFLRPVKGNAILFFSVHLNASPDKSSYHTRSPI 240
           DSA GGEMLFPESKVKSKFWS RRKK NFLRPVKGNAILFFSVHLNASPDKSSYH R PI
Sbjct: 181 DSASGGEMLFPESKVKSKFWSGRRKKKNFLRPVKGNAILFFSVHLNASPDKSSYHIRYPI 240

Query: 241 LNGELWVATKFFYLRPTTGNKRTVESDVDGCIDEDKSCPQWAAIGECERNTVFMIGSPDY 300
            NGELWVATKF YLRP TGNK T++S++DGCIDEDKSCPQWAAIGECERN VFM+GSPDY
Sbjct: 241 RNGELWVATKFLYLRPPTGNKHTIDSNIDGCIDEDKSCPQWAAIGECERNAVFMVGSPDY 300

Query: 301 YGTCRKSCNAC 311
           YGTCRKSCNAC
Sbjct: 301 YGTCRKSCNAC 311

[Close]
BLAST of Bhi12G000444 vs. NCBI nr
Match: KAA0043468.1 (putative prolyl 4-hydroxylase 12 [Cucumis melo var. makuwa])

HSP 1 Score: 575.9 bits (1483), Expect = 2.1e-160
Identity = 281/311 (90.35%), Postives = 293/311 (94.21%), Query Frame = 0 

Query: 1   MDSRLNFLLLLATAFSFSTCLAQSNLISGRKGLRDQLVDRPLSYSNHSGRIDPSRVVQVS 60
           MDSRLNFLLL ATAFSFSTCLAQSNLISGRKGLRDQLVDRPLSYSN S RIDPSRVVQVS
Sbjct: 1   MDSRLNFLLLFATAFSFSTCLAQSNLISGRKGLRDQLVDRPLSYSNQSVRIDPSRVVQVS 60

Query: 61  WQPRVFLYKGFLSDEECDHLISLASNSEDNPSGNSAGSGNTVSTKLLNSSGVILNTSDDI 120
           W+PRVFLYKGFLSD+ECDHLISLASNS+DNPS NSAGSGNTVST+LLN SGVILNT+DDI
Sbjct: 61  WRPRVFLYKGFLSDDECDHLISLASNSKDNPSRNSAGSGNTVSTELLNGSGVILNTTDDI 120

Query: 121 IARIENQIAVWTFLPKDHGMPFQIMQYRGEEAEHKYFYGNGSAM-SSSEPLMATVVLYLS 180
           IARIEN+IAVWT LPKDHGMPFQIMQYRGEEA+HKYFYGN SAM SSSEPLMATVVLYLS
Sbjct: 121 IARIENRIAVWTLLPKDHGMPFQIMQYRGEEAKHKYFYGNRSAMSSSSEPLMATVVLYLS 180

Query: 181 DSARGGEMLFPESKVKSKFWSDRRKKNNFLRPVKGNAILFFSVHLNASPDKSSYHTRSPI 240
           DSA GGEMLFPESKVKSKFWS RRKK NFLRPVKGNAILFFSVHLNASPDKSSYH R PI
Sbjct: 181 DSASGGEMLFPESKVKSKFWSGRRKKKNFLRPVKGNAILFFSVHLNASPDKSSYHIRYPI 240

Query: 241 LNGELWVATKFFYLRPTTGNKRTVESDVDGCIDEDKSCPQWAAIGECERNTVFMIGSPDY 300
            NGELWVATKF YLRP TGNK T++S++DGCIDEDKSCPQWAAIGECERN VFM+GSPDY
Sbjct: 241 RNGELWVATKFLYLRPPTGNKHTIDSNIDGCIDEDKSCPQWAAIGECERNAVFMVGSPDY 300

Query: 301 YGTCRKSCNAC 311
           YGTCRKSCNAC
Sbjct: 301 YGTCRKSCNAC 311

[Close]
BLAST of Bhi12G000444 vs. NCBI nr
Match: XP_004152378.1 (probable prolyl 4-hydroxylase 12 [Cucumis sativus] >KGN49777.2 hypothetical protein Csa_000298 [Cucumis sativus])

HSP 1 Score: 572.4 bits (1474), Expect = 2.4e-159
Identity = 278/311 (89.39%), Postives = 293/311 (94.21%), Query Frame = 0 

Query: 1   MDSRLNFLLLLATAFSFSTCLAQSNLISGRKGLRDQLVDRPLSYSNHSGRIDPSRVVQVS 60
           MDSRLNFLLLLATAFSFSTCLAQSNLISGRKGLRD+LVDRPLSYSN+SGRIDPSRVVQVS
Sbjct: 1   MDSRLNFLLLLATAFSFSTCLAQSNLISGRKGLRDRLVDRPLSYSNYSGRIDPSRVVQVS 60

Query: 61  WQPRVFLYKGFLSDEECDHLISLASNSEDNPSGNSAGSGNTVSTKLLNSSGVILNTSDDI 120
           W+PRVFLYKGFLSDEECDHLISLASNSEDNPS NSAGSG TVST+LLNSSGVILNT+DDI
Sbjct: 61  WRPRVFLYKGFLSDEECDHLISLASNSEDNPSRNSAGSGITVSTELLNSSGVILNTTDDI 120

Query: 121 IARIENQIAVWTFLPKDHGMPFQIMQYRGEEAEHKYFYGNGSAM-SSSEPLMATVVLYLS 180
           +ARIEN++A+WT LPKDH MPFQIMQYRGEEA+HKYFYGN SAM  SSEPLMATVVLYLS
Sbjct: 121 VARIENRLAIWTLLPKDHSMPFQIMQYRGEEAKHKYFYGNRSAMLPSSEPLMATVVLYLS 180

Query: 181 DSARGGEMLFPESKVKSKFWSDRRKKNNFLRPVKGNAILFFSVHLNASPDKSSYHTRSPI 240
           DSA GGE+LFPESKVKSKFWS RRKKNNFLRPVKGNAILFFSVHLNASPDKSSYH RSPI
Sbjct: 181 DSASGGEILFPESKVKSKFWSGRRKKNNFLRPVKGNAILFFSVHLNASPDKSSYHIRSPI 240

Query: 241 LNGELWVATKFFYLRPTTGNKRTVESDVDGCIDEDKSCPQWAAIGECERNTVFMIGSPDY 300
            +GELWVATKF YL P  GNK T++SDVDGC DEDKSCPQWAAIGECERN VFM+GSPDY
Sbjct: 241 RDGELWVATKFLYLGPPAGNKHTIQSDVDGCFDEDKSCPQWAAIGECERNAVFMVGSPDY 300

Query: 301 YGTCRKSCNAC 311
           YGTCRKSCNAC
Sbjct: 301 YGTCRKSCNAC 311

[Close]
BLAST of Bhi12G000444 vs. NCBI nr
Match: XP_022159842.1 (probable prolyl 4-hydroxylase 12 [Momordica charantia])

HSP 1 Score: 534.3 bits (1375), Expect = 7.2e-148
Identity = 264/311 (84.89%), Postives = 280/311 (90.03%), Query Frame = 0 

Query: 1   MDSRLNFLLLLATAFSFSTCLAQSNLISGRKGLRDQLVDR-PLSYSNHSGRIDPSRVVQV 60
           MDSRL  LLLLATA SF +CLAQSNLISGRKGLRDQL++  PLSYSNHSGRIDPSRVVQV
Sbjct: 1   MDSRLPVLLLLATAISFLSCLAQSNLISGRKGLRDQLIESVPLSYSNHSGRIDPSRVVQV 60

Query: 61  SWQPRVFLYKGFLSDEECDHLISLASNSEDNPSGNSAGSGNTVSTKLLNSSGVILNTSDD 120
           SW+PRVFLYKGFLSDEECDHLISLA++SED PSGNS  SGNTV TK+L SSG ILNT+DD
Sbjct: 61  SWRPRVFLYKGFLSDEECDHLISLATSSEDKPSGNSTDSGNTVPTKILKSSGAILNTTDD 120

Query: 121 IIARIENQIAVWTFLPKDHGMPFQIMQYRGEEAEHKYFYGNGSAMSSSEPLMATVVLYLS 180
           IIARIEN+IAVWTFLPKD+ MP QI+QY GEEAEHKY +GN SAM SSEPLMATVVLYLS
Sbjct: 121 IIARIENRIAVWTFLPKDYSMPLQILQYGGEEAEHKYVFGNRSAMLSSEPLMATVVLYLS 180

Query: 181 DSARGGEMLFPESKVKSKFWSDRRKKNNFLRPVKGNAILFFSVHLNASPDKSSYHTRSPI 240
           DSA GGEM FPESKVKS+FWSDRRKKNN LRPVKGNA+L FSVHLNASPDKSS HTRSPI
Sbjct: 181 DSASGGEMRFPESKVKSRFWSDRRKKNNILRPVKGNAVLIFSVHLNASPDKSSSHTRSPI 240

Query: 241 LNGELWVATKFFYLRPTTGNKRTVESDVDGCIDEDKSCPQWAAIGECERNTVFMIGSPDY 300
           L+GELW+ATKFFYLRP TGNK T E D D C DEDKSCPQWAAIGECERN VFMIGSPDY
Sbjct: 241 LDGELWIATKFFYLRPITGNKHTDEPDGD-CNDEDKSCPQWAAIGECERNAVFMIGSPDY 300

Query: 301 YGTCRKSCNAC 311
           YGTCRKSCNAC
Sbjct: 301 YGTCRKSCNAC 310

[Close]
BLAST of Bhi12G000444 vs. ExPASy TrEMBL
Match: A0A1S3AT39 (Procollagen-proline 4-dioxygenase OS=Cucumis melo OX=3656 GN=LOC103482556 PE=3 SV=1)

HSP 1 Score: 578.6 bits (1490), Expect = 1.6e-161
Identity = 283/311 (91.00%), Postives = 293/311 (94.21%), Query Frame = 0 

Query: 1   MDSRLNFLLLLATAFSFSTCLAQSNLISGRKGLRDQLVDRPLSYSNHSGRIDPSRVVQVS 60
           MDSRLNFLLL ATAFSFSTCLAQSNLISGRKGLRDQLVDRPLSYSN S RIDPSRVVQVS
Sbjct: 1   MDSRLNFLLLFATAFSFSTCLAQSNLISGRKGLRDQLVDRPLSYSNQSVRIDPSRVVQVS 60

Query: 61  WQPRVFLYKGFLSDEECDHLISLASNSEDNPSGNSAGSGNTVSTKLLNSSGVILNTSDDI 120
           W+PRVFLYKGFLSDEECDHLISLASNSEDNPS NSAGSGNTVST+LLN SGVILNT+DDI
Sbjct: 61  WRPRVFLYKGFLSDEECDHLISLASNSEDNPSRNSAGSGNTVSTELLNGSGVILNTTDDI 120

Query: 121 IARIENQIAVWTFLPKDHGMPFQIMQYRGEEAEHKYFYGNGSAM-SSSEPLMATVVLYLS 180
           IARIEN+IAVWT LPKDHGMPFQIMQYRGEEA+HKYFYGN SAM SSSEPLMATVVLYLS
Sbjct: 121 IARIENRIAVWTLLPKDHGMPFQIMQYRGEEAKHKYFYGNRSAMSSSSEPLMATVVLYLS 180

Query: 181 DSARGGEMLFPESKVKSKFWSDRRKKNNFLRPVKGNAILFFSVHLNASPDKSSYHTRSPI 240
           DSA GGEMLFPESKVKSKFWS RRKK NFLRPVKGNAILFFSVHLNASPDKSSYH R PI
Sbjct: 181 DSASGGEMLFPESKVKSKFWSGRRKKKNFLRPVKGNAILFFSVHLNASPDKSSYHIRYPI 240

Query: 241 LNGELWVATKFFYLRPTTGNKRTVESDVDGCIDEDKSCPQWAAIGECERNTVFMIGSPDY 300
            NGELWVATKF YLRP TGNK T++S++DGCIDEDKSCPQWAAIGECERN VFM+GSPDY
Sbjct: 241 RNGELWVATKFLYLRPPTGNKHTIDSNIDGCIDEDKSCPQWAAIGECERNAVFMVGSPDY 300

Query: 301 YGTCRKSCNAC 311
           YGTCRKSCNAC
Sbjct: 301 YGTCRKSCNAC 311

[Close]
BLAST of Bhi12G000444 vs. ExPASy TrEMBL
Match: A0A5A7TKX1 (Procollagen-proline 4-dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1167G00060 PE=3 SV=1)

HSP 1 Score: 575.9 bits (1483), Expect = 1.0e-160
Identity = 281/311 (90.35%), Postives = 293/311 (94.21%), Query Frame = 0 

Query: 1   MDSRLNFLLLLATAFSFSTCLAQSNLISGRKGLRDQLVDRPLSYSNHSGRIDPSRVVQVS 60
           MDSRLNFLLL ATAFSFSTCLAQSNLISGRKGLRDQLVDRPLSYSN S RIDPSRVVQVS
Sbjct: 1   MDSRLNFLLLFATAFSFSTCLAQSNLISGRKGLRDQLVDRPLSYSNQSVRIDPSRVVQVS 60

Query: 61  WQPRVFLYKGFLSDEECDHLISLASNSEDNPSGNSAGSGNTVSTKLLNSSGVILNTSDDI 120
           W+PRVFLYKGFLSD+ECDHLISLASNS+DNPS NSAGSGNTVST+LLN SGVILNT+DDI
Sbjct: 61  WRPRVFLYKGFLSDDECDHLISLASNSKDNPSRNSAGSGNTVSTELLNGSGVILNTTDDI 120

Query: 121 IARIENQIAVWTFLPKDHGMPFQIMQYRGEEAEHKYFYGNGSAM-SSSEPLMATVVLYLS 180
           IARIEN+IAVWT LPKDHGMPFQIMQYRGEEA+HKYFYGN SAM SSSEPLMATVVLYLS
Sbjct: 121 IARIENRIAVWTLLPKDHGMPFQIMQYRGEEAKHKYFYGNRSAMSSSSEPLMATVVLYLS 180

Query: 181 DSARGGEMLFPESKVKSKFWSDRRKKNNFLRPVKGNAILFFSVHLNASPDKSSYHTRSPI 240
           DSA GGEMLFPESKVKSKFWS RRKK NFLRPVKGNAILFFSVHLNASPDKSSYH R PI
Sbjct: 181 DSASGGEMLFPESKVKSKFWSGRRKKKNFLRPVKGNAILFFSVHLNASPDKSSYHIRYPI 240

Query: 241 LNGELWVATKFFYLRPTTGNKRTVESDVDGCIDEDKSCPQWAAIGECERNTVFMIGSPDY 300
            NGELWVATKF YLRP TGNK T++S++DGCIDEDKSCPQWAAIGECERN VFM+GSPDY
Sbjct: 241 RNGELWVATKFLYLRPPTGNKHTIDSNIDGCIDEDKSCPQWAAIGECERNAVFMVGSPDY 300

Query: 301 YGTCRKSCNAC 311
           YGTCRKSCNAC
Sbjct: 301 YGTCRKSCNAC 311

[Close]
BLAST of Bhi12G000444 vs. ExPASy TrEMBL
Match: A0A6J1E0X9 (Procollagen-proline 4-dioxygenase OS=Momordica charantia OX=3673 GN=LOC111026141 PE=3 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 3.5e-148
Identity = 264/311 (84.89%), Postives = 280/311 (90.03%), Query Frame = 0 

Query: 1   MDSRLNFLLLLATAFSFSTCLAQSNLISGRKGLRDQLVDR-PLSYSNHSGRIDPSRVVQV 60
           MDSRL  LLLLATA SF +CLAQSNLISGRKGLRDQL++  PLSYSNHSGRIDPSRVVQV
Sbjct: 1   MDSRLPVLLLLATAISFLSCLAQSNLISGRKGLRDQLIESVPLSYSNHSGRIDPSRVVQV 60

Query: 61  SWQPRVFLYKGFLSDEECDHLISLASNSEDNPSGNSAGSGNTVSTKLLNSSGVILNTSDD 120
           SW+PRVFLYKGFLSDEECDHLISLA++SED PSGNS  SGNTV TK+L SSG ILNT+DD
Sbjct: 61  SWRPRVFLYKGFLSDEECDHLISLATSSEDKPSGNSTDSGNTVPTKILKSSGAILNTTDD 120

Query: 121 IIARIENQIAVWTFLPKDHGMPFQIMQYRGEEAEHKYFYGNGSAMSSSEPLMATVVLYLS 180
           IIARIEN+IAVWTFLPKD+ MP QI+QY GEEAEHKY +GN SAM SSEPLMATVVLYLS
Sbjct: 121 IIARIENRIAVWTFLPKDYSMPLQILQYGGEEAEHKYVFGNRSAMLSSEPLMATVVLYLS 180

Query: 181 DSARGGEMLFPESKVKSKFWSDRRKKNNFLRPVKGNAILFFSVHLNASPDKSSYHTRSPI 240
           DSA GGEM FPESKVKS+FWSDRRKKNN LRPVKGNA+L FSVHLNASPDKSS HTRSPI
Sbjct: 181 DSASGGEMRFPESKVKSRFWSDRRKKNNILRPVKGNAVLIFSVHLNASPDKSSSHTRSPI 240

Query: 241 LNGELWVATKFFYLRPTTGNKRTVESDVDGCIDEDKSCPQWAAIGECERNTVFMIGSPDY 300
           L+GELW+ATKFFYLRP TGNK T E D D C DEDKSCPQWAAIGECERN VFMIGSPDY
Sbjct: 241 LDGELWIATKFFYLRPITGNKHTDEPDGD-CNDEDKSCPQWAAIGECERNAVFMIGSPDY 300

Query: 301 YGTCRKSCNAC 311
           YGTCRKSCNAC
Sbjct: 301 YGTCRKSCNAC 310

[Close]
BLAST of Bhi12G000444 vs. ExPASy TrEMBL
Match: A0A0A0KPE4 (Procollagen-proline 4-dioxygenase OS=Cucumis sativus OX=3659 GN=Csa_5G166460 PE=3 SV=1)

HSP 1 Score: 531.2 bits (1367), Expect = 2.9e-147
Identity = 255/289 (88.24%), Postives = 271/289 (93.77%), Query Frame = 0 

Query: 23  QSNLISGRKGLRDQLVDRPLSYSNHSGRIDPSRVVQVSWQPRVFLYKGFLSDEECDHLIS 82
           +SNLISGRKGLRD+LVDRPLSYSN+SGRIDPSRVVQVSW+PRVFLYKGFLSDEECDHLIS
Sbjct: 6   KSNLISGRKGLRDRLVDRPLSYSNYSGRIDPSRVVQVSWRPRVFLYKGFLSDEECDHLIS 65

Query: 83  LASNSEDNPSGNSAGSGNTVSTKLLNSSGVILNTSDDIIARIENQIAVWTFLPKDHGMPF 142
           LASNSEDNPS NSAGSG TVST+LLNSSGVILNT+DDI+ARIEN++A+WT LPKDH MPF
Sbjct: 66  LASNSEDNPSRNSAGSGITVSTELLNSSGVILNTTDDIVARIENRLAIWTLLPKDHSMPF 125

Query: 143 QIMQYRGEEAEHKYFYGNGSAM-SSSEPLMATVVLYLSDSARGGEMLFPESKVKSKFWSD 202
           QIMQYRGEEA+HKYFYGN SAM  SSEPLMATVVLYLSDSA GGE+LFPESKVKSKFWS 
Sbjct: 126 QIMQYRGEEAKHKYFYGNRSAMLPSSEPLMATVVLYLSDSASGGEILFPESKVKSKFWSG 185

Query: 203 RRKKNNFLRPVKGNAILFFSVHLNASPDKSSYHTRSPILNGELWVATKFFYLRPTTGNKR 262
           RRKKNNFLRPVKGNAILFFSVHLNASPDKSSYH RSPI +GELWVATKF YL P  GNK 
Sbjct: 186 RRKKNNFLRPVKGNAILFFSVHLNASPDKSSYHIRSPIRDGELWVATKFLYLGPPAGNKH 245

Query: 263 TVESDVDGCIDEDKSCPQWAAIGECERNTVFMIGSPDYYGTCRKSCNAC 311
           T++SDVDGC DEDKSCPQWAAIGECERN VFM+GSPDYYGTCRKSCNAC
Sbjct: 246 TIQSDVDGCFDEDKSCPQWAAIGECERNAVFMVGSPDYYGTCRKSCNAC 294

[Close]
BLAST of Bhi12G000444 vs. ExPASy TrEMBL
Match: A0A6J1E2P0 (Procollagen-proline 4-dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111430280 PE=3 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 5.2e-144
Identity = 258/314 (82.17%), Postives = 281/314 (89.49%), Query Frame = 0 

Query: 1   MDSRLNFLLLLATAFSFSTCLAQSNLISGRKGLRDQLVDR-PLSYSNHSGRIDPSRVVQV 60
           MDSRL FLLLLA AFSFS+CLAQSN ISGRKGLRDQ+V+   LSYSNHS RIDPSRVVQ+
Sbjct: 1   MDSRLTFLLLLAAAFSFSSCLAQSNSISGRKGLRDQMVNSGHLSYSNHSERIDPSRVVQI 60

Query: 61  SWQPRVFLYKGFLSDEECDHLISLASNSEDNPSGNSAGSGNTVSTKLLNSSGVILNTSDD 120
           SWQPR FLYKGFLSDEECDHLI+LASNSED PS N+AGS NTVSTK L +SG ILNT+DD
Sbjct: 61  SWQPRAFLYKGFLSDEECDHLIALASNSEDKPSRNNAGSRNTVSTKFLGNSGAILNTTDD 120

Query: 121 IIARIENQIAVWTFLPKDHGMPFQIMQYRGEEAE-HKYFYGNGSAMSSSEPLMATVVLYL 180
           II RIEN+IAVWTFLPKDH MPFQIM+Y GEEA  HKYF+GN SAM SSEPLMATVVLYL
Sbjct: 121 IIGRIENRIAVWTFLPKDHSMPFQIMKYGGEEAAGHKYFFGNRSAMPSSEPLMATVVLYL 180

Query: 181 SDSARGGEMLFPESKVKSKFWSDRRKKNNFLRPVKGNAILFFSVHLNASPDKSSYHTRSP 240
           SDSA GGE+LFP SKVK +FWSDRRKKNNFLRPVKGNA+LFFSVHLNASPDKS YH+R+P
Sbjct: 181 SDSASGGEILFPVSKVKRRFWSDRRKKNNFLRPVKGNAVLFFSVHLNASPDKSCYHSRTP 240

Query: 241 ILNGELWVATKFFYLRP-TTGNKRTVESDV-DGCIDEDKSCPQWAAIGECERNTVFMIGS 300
           IL+G+LWVATKFFY+RP  TGN+  VES V D CIDED+SCP+WAAIGEC+RN VFMIGS
Sbjct: 241 ILDGKLWVATKFFYIRPAATGNEHAVESGVDDDCIDEDESCPKWAAIGECKRNAVFMIGS 300

Query: 301 PDYYGTCRKSCNAC 311
           PDYYGTCRKSCNAC
Sbjct: 301 PDYYGTCRKSCNAC 314

The following BLAST results are available for this feature:
BLAST of Bhi12G000444 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G25600.18.1e-6544.84Oxoglutarate/iron-dependent oxygenase [more]
AT3G28490.12.1e-5741.67Oxoglutarate/iron-dependent oxygenase [more]
AT3G28480.11.1e-5638.08Oxoglutarate/iron-dependent oxygenase [more]
AT3G28480.29.0e-5638.86Oxoglutarate/iron-dependent oxygenase [more]
AT3G06300.11.4e-4837.05P4H isoform 2 [more]
BLAST of Bhi12G000444 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8GXT71.1e-6344.84Probable prolyl 4-hydroxylase 12 OS=Arabidopsis thaliana OX=3702 GN=P4H12 PE=2 S... [more]
F4J0A83.0e-5641.67Probable prolyl 4-hydroxylase 6 OS=Arabidopsis thaliana OX=3702 GN=P4H6 PE=2 SV=... [more]
Q8L9701.5e-5538.08Probable prolyl 4-hydroxylase 7 OS=Arabidopsis thaliana OX=3702 GN=P4H7 PE=2 SV=... [more]
F4JAU32.0e-4737.05Prolyl 4-hydroxylase 2 OS=Arabidopsis thaliana OX=3702 GN=P4H2 PE=1 SV=1[more]
Q8LAN34.3e-4735.61Probable prolyl 4-hydroxylase 4 OS=Arabidopsis thaliana OX=3702 GN=P4H4 PE=2 SV=... [more]
BLAST of Bhi12G000444 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038906497.12.3e-178100.00probable prolyl 4-hydroxylase 12 [Benincasa hispida][more]
XP_008436994.13.3e-16191.00PREDICTED: probable prolyl 4-hydroxylase 12 [Cucumis melo][more]
KAA0043468.12.1e-16090.35putative prolyl 4-hydroxylase 12 [Cucumis melo var. makuwa][more]
XP_004152378.12.4e-15989.39probable prolyl 4-hydroxylase 12 [Cucumis sativus] >KGN49777.2 hypothetical prot... [more]
XP_022159842.17.2e-14884.89probable prolyl 4-hydroxylase 12 [Momordica charantia][more]
BLAST of Bhi12G000444 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A1S3AT391.6e-16191.00Procollagen-proline 4-dioxygenase OS=Cucumis melo OX=3656 GN=LOC103482556 PE=3 S... [more]
A0A5A7TKX11.0e-16090.35Procollagen-proline 4-dioxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
A0A6J1E0X93.5e-14884.89Procollagen-proline 4-dioxygenase OS=Momordica charantia OX=3673 GN=LOC111026141... [more]
A0A0A0KPE42.9e-14788.24Procollagen-proline 4-dioxygenase OS=Cucumis sativus OX=3659 GN=Csa_5G166460 PE=... [more]
A0A6J1E2P05.2e-14482.17Procollagen-proline 4-dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111430280 ... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003582ShKT domainSMARTSM00254ShkT_1coord: 269..310
e-value: 1.4E-4
score: 31.2
IPR003582ShKT domainPROSITEPS51670SHKTcoord: 270..310
score: 8.886379
IPR006620Prolyl 4-hydroxylase, alpha subunitSMARTSM00702p4hccoord: 63..252
e-value: 2.5E-19
score: 80.2
NoneNo IPR availableGENE3D2.60.120.620q2cbj1_9rhob like domaincoord: 55..252
e-value: 2.3E-39
score: 137.3
NoneNo IPR availablePANTHERPTHR10869:SF102PROLYL 4-HYDROXYLASE 12-RELATEDcoord: 1..310
IPR045054Prolyl 4-hydroxylasePANTHERPTHR10869PROLYL 4-HYDROXYLASE ALPHA SUBUNITcoord: 1..310

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi12M000444Bhi12M000444mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019511 peptidyl-proline hydroxylation
cellular_component GO:0005789 endoplasmic reticulum membrane
molecular_function GO:0005506 iron ion binding
molecular_function GO:0031418 L-ascorbic acid binding
molecular_function GO:0004656 procollagen-proline 4-dioxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen