Bhi12G000442 (gene) Wax gourd (B227) v1

Overview
NameBhi12G000442
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionUPF0496 protein At3g49070
Locationchr12: 15616517 .. 15618005 (-)
RNA-Seq ExpressionBhi12G000442
SyntenyBhi12G000442
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCAAGAGCATGGCATTCAATTCAAACAGGTTTTTAGATTTTTACTTAAATAAATATTTAACTTTATAATTCATGTTCTATACAAAATTAAACTTACTAAAACATGTATATTAAGGCATGTTTGAGTAAAAATAATTAAGGCAAAATACAAATAGGTCCTAATAATTAAGGTGGCAATCACACATTATAAAGTTCTAGACTGCATTTTTTTTAAAACCCTTTAGCTAGTGAATAAAATTAACAACCACATCAACAATGAATTAGAAATAATTGTCTTGATTTGTAAAAAAATATTGGAGCTAGGCACGTGTAATTAAGGCATATTTGTTATAATTATCAATAACTTTGTTGGCTAGAAGTTCAAAATCCATAAGATATGATATTACACTTTATTTTACATATTTTCATGTTGAATAGATGGTGAACCACACGTTACAAGTCATTCAGTAGACATAGATGTGAGGGAGGAGTACGCGAATGCCTTCCGTACTGAATCGTACATCGACTTTTGGACCCGTGTTCTTGCCTTAAACAATGGAGATGACCTCACAACCCAACTTTCAGTAGAGTCCACAACCGCAACCCACCTTTCATCTTATAGACTATTTGTCGAACATCTACTTGACCCACCTCAATCCACAATTAAAAGAATTCTAACTTCGCCCCATCTTGGAGCCAATTCTTGCTCCCTTCTCTTAGATTACTTCTCTCACACGGCCAATGCCTCTCTCTCATGTAGTCATTTATTAAAACACATCAACCACTTACGTCTCAAACTCCATCCCTTAGAAATCACCCTCCAATCCTTAGAAAACAAAGAAAAATTTCATCATGAGTCCCATTTCAAACAACTCTTAATTCGTTTGGTTGAATTCTCCAACACCCGCAATCCATTTATACCATCTATAGAACAGGTTCAAATCATTCAAAATGGATGCTCCAAATTGTTAAAGCAACTTGAGTTTAGTCGCGACAAGGCTCAAACCAAGCTCAAGAGAGTTAGATATTTTCAACATAGTTCAGCTGGCTTTTTGGTGGCTGTAACTGCATCACTTACGGTAATAATCGTGACTCATGGAATTGCATTATTTGTTGCTGCACCTGGCTTTCTTGTGGGTGCTATAAAGTTGGCTAATCAATTGAGGAAGCTAGTTAAGGATGTTGCTCGACTTAATATTACTGCAAAAGGGACTTACACTTTAAATAGAGATTTCGATACGATTGGTAGGCTCGTGGCTCGACTCAATCATGAGCTCGAACATATGAGGGTGATGGCAAGATTTTGGCTTGATAGAGGGGAAGATAAACGTCGGGCCATTGGTGAATTAGTGCGACAATTAAATCAAAGTCATGTGAACTTTAGCCACCAATTGGATGAGCTTGAGGAGCATTTGTATTTGTGTTTTATGACCATAAATAGAGCTAGAAATCTTGTAGTGAAAGAGATTTTGGATTCGGGTCAACCTATAAAGATTTCGTATTTATGA

mRNA sequence

ATGCCAAGAGCATGGCATTCAATTCAAACAGATGGTGAACCACACGTTACAAGTCATTCAGTAGACATAGATGTGAGGGAGGAGTACGCGAATGCCTTCCGTACTGAATCGTACATCGACTTTTGGACCCGTGTTCTTGCCTTAAACAATGGAGATGACCTCACAACCCAACTTTCAGTAGAGTCCACAACCGCAACCCACCTTTCATCTTATAGACTATTTGTCGAACATCTACTTGACCCACCTCAATCCACAATTAAAAGAATTCTAACTTCGCCCCATCTTGGAGCCAATTCTTGCTCCCTTCTCTTAGATTACTTCTCTCACACGGCCAATGCCTCTCTCTCATGTAGTCATTTATTAAAACACATCAACCACTTACGTCTCAAACTCCATCCCTTAGAAATCACCCTCCAATCCTTAGAAAACAAAGAAAAATTTCATCATGAGTCCCATTTCAAACAACTCTTAATTCGTTTGGTTGAATTCTCCAACACCCGCAATCCATTTATACCATCTATAGAACAGGTTCAAATCATTCAAAATGGATGCTCCAAATTGTTAAAGCAACTTGAGTTTAGTCGCGACAAGGCTCAAACCAAGCTCAAGAGAGTTAGATATTTTCAACATAGTTCAGCTGGCTTTTTGGTGGCTGTAACTGCATCACTTACGGTAATAATCGTGACTCATGGAATTGCATTATTTGTTGCTGCACCTGGCTTTCTTGTGGGTGCTATAAAGTTGGCTAATCAATTGAGGAAGCTAGTTAAGGATGTTGCTCGACTTAATATTACTGCAAAAGGGACTTACACTTTAAATAGAGATTTCGATACGATTGGTAGGCTCGTGGCTCGACTCAATCATGAGCTCGAACATATGAGGGTGATGGCAAGATTTTGGCTTGATAGAGGGGAAGATAAACGTCGGGCCATTGGTGAATTAGTGCGACAATTAAATCAAAGTCATGTGAACTTTAGCCACCAATTGGATGAGCTTGAGGAGCATTTGTATTTGTGTTTTATGACCATAAATAGAGCTAGAAATCTTGTAGTGAAAGAGATTTTGGATTCGGGTCAACCTATAAAGATTTCGTATTTATGA

Coding sequence (CDS)

ATGCCAAGAGCATGGCATTCAATTCAAACAGATGGTGAACCACACGTTACAAGTCATTCAGTAGACATAGATGTGAGGGAGGAGTACGCGAATGCCTTCCGTACTGAATCGTACATCGACTTTTGGACCCGTGTTCTTGCCTTAAACAATGGAGATGACCTCACAACCCAACTTTCAGTAGAGTCCACAACCGCAACCCACCTTTCATCTTATAGACTATTTGTCGAACATCTACTTGACCCACCTCAATCCACAATTAAAAGAATTCTAACTTCGCCCCATCTTGGAGCCAATTCTTGCTCCCTTCTCTTAGATTACTTCTCTCACACGGCCAATGCCTCTCTCTCATGTAGTCATTTATTAAAACACATCAACCACTTACGTCTCAAACTCCATCCCTTAGAAATCACCCTCCAATCCTTAGAAAACAAAGAAAAATTTCATCATGAGTCCCATTTCAAACAACTCTTAATTCGTTTGGTTGAATTCTCCAACACCCGCAATCCATTTATACCATCTATAGAACAGGTTCAAATCATTCAAAATGGATGCTCCAAATTGTTAAAGCAACTTGAGTTTAGTCGCGACAAGGCTCAAACCAAGCTCAAGAGAGTTAGATATTTTCAACATAGTTCAGCTGGCTTTTTGGTGGCTGTAACTGCATCACTTACGGTAATAATCGTGACTCATGGAATTGCATTATTTGTTGCTGCACCTGGCTTTCTTGTGGGTGCTATAAAGTTGGCTAATCAATTGAGGAAGCTAGTTAAGGATGTTGCTCGACTTAATATTACTGCAAAAGGGACTTACACTTTAAATAGAGATTTCGATACGATTGGTAGGCTCGTGGCTCGACTCAATCATGAGCTCGAACATATGAGGGTGATGGCAAGATTTTGGCTTGATAGAGGGGAAGATAAACGTCGGGCCATTGGTGAATTAGTGCGACAATTAAATCAAAGTCATGTGAACTTTAGCCACCAATTGGATGAGCTTGAGGAGCATTTGTATTTGTGTTTTATGACCATAAATAGAGCTAGAAATCTTGTAGTGAAAGAGATTTTGGATTCGGGTCAACCTATAAAGATTTCGTATTTATGA

Protein sequence

MPRAWHSIQTDGEPHVTSHSVDIDVREEYANAFRTESYIDFWTRVLALNNGDDLTTQLSVESTTATHLSSYRLFVEHLLDPPQSTIKRILTSPHLGANSCSLLLDYFSHTANASLSCSHLLKHINHLRLKLHPLEITLQSLENKEKFHHESHFKQLLIRLVEFSNTRNPFIPSIEQVQIIQNGCSKLLKQLEFSRDKAQTKLKRVRYFQHSSAGFLVAVTASLTVIIVTHGIALFVAAPGFLVGAIKLANQLRKLVKDVARLNITAKGTYTLNRDFDTIGRLVARLNHELEHMRVMARFWLDRGEDKRRAIGELVRQLNQSHVNFSHQLDELEEHLYLCFMTINRARNLVVKEILDSGQPIKISYL
Homology
BLAST of Bhi12G000442 vs. TAIR 10
Match: AT3G49070.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 303.9 bits (777), Expect = 1.7e-82
Identity = 174/350 (49.71%), Postives = 222/350 (63.43%), Query Frame = 0

Query: 22  DIDVREEYANAFRTESYIDFWTRVLALNNGDDLTTQLS-----VESTTATHLSSYRLFVE 81
           D+DVREEYANAFRTESY  FWTRV+ L+      +  S       S+T+  L SYRLF  
Sbjct: 68  DVDVREEYANAFRTESYNHFWTRVVQLSRKKSAVSSSSSSPPIESSSTSARLMSYRLFAH 127

Query: 82  HLLDPPQSTIKRILTSPHLGANSCSLLLDYFSHTANASLSCSHLLKHINHLRLKLHPLEI 141
           +LLDP  +TI RIL    +G ++ +LL DYF  TANA L C+ LLK+I+HLR K   L  
Sbjct: 128 NLLDPDLNTITRILDVSRVGRHTRTLLSDYFLETANAFLLCTQLLKNIHHLRSKYESL-- 187

Query: 142 TLQSLENKEKFHHESHFKQLLI-RLVEFSNTRNPFIPSIEQVQIIQNGCSKLLKQLEFSR 201
                  K KFH E+H    LI +  E S   +PFI S  ++Q+I++GC  LLK+LE  R
Sbjct: 188 -------KPKFHSENHNSLALIDQFTEISKWFDPFISSGSRIQLIRSGCLYLLKRLESRR 247

Query: 202 DKAQTKLKRVRYFQHSSAGFLVAVTASLTVIIVTHGIALFVAAPGFLVGAIKLANQLRKL 261
           DK + KLK +    HSS   ++A+T +L V I +H  ALF+AAP  L    K A    KL
Sbjct: 248 DKTRAKLKLINGLTHSSGLLVLALTTTLIVTIASHAFALFLAAPTLLASQFKPAGLRNKL 307

Query: 262 VKDVARLNITAKGTYTLNRDFDTIGRLVARLNHELEHMRVMARFWLDRGEDKRRAIGELV 321
            K  ARL++ AKGTY L+RD DTI RLV R+N E+ H+R MA FW+ RG  + R   E+ 
Sbjct: 308 TKTAARLDVAAKGTYILSRDLDTISRLVTRINDEVNHVRAMAEFWVGRGSGRVRGSEEVA 367

Query: 322 RQLNQSHVNFSHQLDELEEHLYLCFMTINRARNLVVKEILDSGQPIKISY 366
           R+L +   +FS +LDELEEH+YLCFMTINRARNL+VKEILDS  P   S+
Sbjct: 368 RELKRCEESFSEELDELEEHIYLCFMTINRARNLLVKEILDSDDPPNCSF 408

BLAST of Bhi12G000442 vs. TAIR 10
Match: AT3G19250.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 121.3 bits (303), Expect = 1.6e-27
Identity = 103/362 (28.45%), Postives = 184/362 (50.83%), Query Frame = 0

Query: 7   SIQTDGEPHVT---SHSVDIDVREEYANAFRTESYIDFWTRVLALNNGDDLTTQLSVEST 66
           S+  D  PH +   S +   ++  E A+AF+T SY D  +R+L ++      TQ ++E  
Sbjct: 15  SLGDDHLPHPSPEGSVASTFNLSHELAHAFQTPSYHDIRSRLLVID-----PTQENLE-- 74

Query: 67  TATHLSSYRLFVEHLLDPPQSTIKRILTSPHLGANS-CSLLLDYFSHTANASLSCSHLLK 126
                    LF+   L P   +++  L+  H    +  +L+  YF H+ +A+  C +L +
Sbjct: 75  ---------LFLSQELRPNNESVQEALSLRHAKQTTLTNLVSTYFQHSEDATRFCLNLYQ 134

Query: 127 HINHLRLKLHPLEITLQSLENKEKFH--HESHFKQLLIRLVEFSNTRNPFI-PSIEQVQI 186
           +++  R  L+   + L ++  ++      ES         ++     NPF  P     Q 
Sbjct: 135 NVHSARCHLYTPLLDLFNIFPRDSHSAIDESFCNLAFDVFLKLDTFENPFASPESHSFQD 194

Query: 187 IQNGCSKLLKQLEFSRDKAQTKLKRVRYFQHSSAGFLVAVTASLTVIIVTHGIALFVAAP 246
            Q    +L  +L+    K+++   RVR   H++AG  + +  ++ V+  +     + A P
Sbjct: 195 TQLCFYQLADKLDTRIRKSKS---RVRLLHHATAGSALCLVTAVVVVAASAAFIAYHALP 254

Query: 247 GFLVGAIKLANQL------RKLVKDVARLNITAKGTYTLNRDFDTIGRLVARLNHELEHM 306
             LV A  L          +K + ++ +LN+ AKGT+ LN+D DTI RLV+RL+  +++ 
Sbjct: 255 TILVVAGPLCTPYLPHSFKKKELSNIFQLNVAAKGTFALNKDLDTIDRLVSRLHTGVKND 314

Query: 307 RVMARFWLDRGEDKRRAIGELVRQLNQSHVNFSHQLDELEEHLYLCFMTINRARNLVVKE 356
           +++ R  L+RG D    I E V+QL +SHVN +HQL+ L +H+   F  +N++R+L++KE
Sbjct: 315 KLLIRLGLERGRDV-YTIPEFVKQLRKSHVNHTHQLEVLVDHICRWFTNVNKSRSLLLKE 356

BLAST of Bhi12G000442 vs. TAIR 10
Match: AT1G20180.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 119.4 bits (298), Expect = 6.1e-27
Identity = 108/361 (29.92%), Postives = 173/361 (47.92%), Query Frame = 0

Query: 23  IDVREEYANAFRTESYIDFWTRV---LALNNGDDLTTQLSVESTTATHLSSYRLFVEHLL 82
           + V EEY  AFRT SY++  T+    L + +   L++  S   ++++ LS +  F ++LL
Sbjct: 34  LSVNEEYKEAFRTNSYLETRTKAEDQLGITSCSKLSSS-SPSPSSSSDLSFHSHFTDYLL 93

Query: 83  DPPQSTIKRILTSPHLGANSCSLLLDYFSHTANASLSCSHLLK-----HINHLRLKLHPL 142
           DPPQ T+  ++    L     +L++ +F  ++ A   C  LL+      INH ++K   +
Sbjct: 94  DPPQETLDALMQDSSLD----NLIVTFFDLSSEACDVCETLLQCLQQIKINHNKIK-RVM 153

Query: 143 EITLQSLENKEKFHHESHFKQLLI--RLVEFSNTRNPFIPSIEQVQ--IIQNGCSKLLKQ 202
           +I  +     +           LI   L  F+  +NP    + + Q  I+ +  S LL +
Sbjct: 154 KIGKRVCNGAKTLECSPEMLCALIFQELSRFAALKNPLCRIVNEAQFRIVHDANSDLLTK 213

Query: 203 LEFSRDKAQTKLKRVRYFQHSSAGFLVAVTASLTV----IIVTHGIALFVAAPGFL---- 262
           L   + + + K++  + F     G+ + +T S  V    II  H I    AAP  L    
Sbjct: 214 LTSKKRRIRRKIRFFK-FCKKLGGYSLVITHSAIVITLLIIALHSILGVFAAPALLGLCS 273

Query: 263 ---------VGAIKLANQLRKLVKDVARLNITAKGTYTLNRDFDTIGRLVARLNHELEHM 322
                     G +  +N+   L K   +++I AKG + L  D DT+ RL  RL  E+EH 
Sbjct: 274 FCLLRKKKAKGRMHKSNKDTTLEKLGTQIDIAAKGMFILINDLDTLSRLAGRLCDEIEHR 333

Query: 323 RVMARFWLDRGEDKRRAIGELVRQLNQSHVNFSHQLDELEEHLYLCFMTINRARNLVVKE 355
           + +A   +     K   + E +R+ N     FS QL ELEEHLYLCF TINR+R LV+ +
Sbjct: 334 KTVAA--MCAKSRKIEVLKEALREFNGHEEKFSDQLQELEEHLYLCFHTINRSRRLVLAQ 385

BLAST of Bhi12G000442 vs. TAIR 10
Match: AT3G19330.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 107.1 bits (266), Expect = 3.1e-23
Identity = 100/354 (28.25%), Postives = 177/354 (50.00%), Query Frame = 0

Query: 20  SVDIDVREEYANAFRTESYIDFWTRVLALNNGDDLTTQLSVESTTATH---LSSYRLFVE 79
           S   ++  E A+AF+T SY D  +RV            + V+ T   H        L + 
Sbjct: 42  SPTFNLSRELAHAFQTPSYHDVRSRV-----------HVVVDLTQIHHRLIQPDIELLLS 101

Query: 80  HLLDPPQSTIKRILTSPHLGANS-CSLLLDYFSHTANASLSCSHLLKHINHLRLKLHPLE 139
            +L P +  ++  +   H+   +  +L+  YF H+ +A+  C +L ++++  R  L+   
Sbjct: 102 QVLQPNKECVQEAIR--HVKQTTLTNLVSTYFQHSEDATRLCLNLYQNVHSARHHLYTPL 161

Query: 140 ITL------QSLENKEKFHHESHFKQLLIRLVEFSNT-RNPFIPSIEQVQIIQNGCSKLL 199
           + L       SL   ++   +  F  + ++L  F N   +P   S    Q+         
Sbjct: 162 LDLFNIFPGDSLPAIDESLCDLAF-DVFLKLDTFENPFSSPESYSFRDTQL-------CF 221

Query: 200 KQLEFSRDKAQTKLK-RVRYFQHSSAG-------FLVAVTASLTVIIVTHGIALFVAAPG 259
            QL+ + D+   K + RVR   H++AG        +VAV AS  V+I +H + + +   G
Sbjct: 222 SQLKHNLDRRLRKSRSRVRLIHHATAGSSLCLVAAVVAVAAS-AVVIASHALPILLVVAG 281

Query: 260 FLVGAIKLANQLRKLVKDVARLNITAKGTYTLNRDFDTIGRLVARLNHELEHMRVMARFW 319
            L       +  RK + ++ +LN  +KGT+ LN+D DTI RLV+RL+  +E+ + + R  
Sbjct: 282 PLCSPYLPHSFKRKELTNICQLNAASKGTFVLNKDLDTIDRLVSRLHTGIEYDKFLIRLG 341

Query: 320 LDRGEDKRRAIGELVRQLNQSHVNFSHQLDELEEHLYLCFMTINRARNLVVKEI 355
           L+RG D   +I E+++ L +SH+  +HQL +LE+H+ L F  +N+AR+L++ EI
Sbjct: 342 LERGRDV-HSIQEILKLLRKSHLPLTHQLKDLEDHICLWFTNVNKARSLLLTEI 372

BLAST of Bhi12G000442 vs. TAIR 10
Match: AT1G20180.2 (Protein of unknown function (DUF677) )

HSP 1 Score: 105.9 bits (263), Expect = 6.9e-23
Identity = 99/345 (28.70%), Postives = 166/345 (48.12%), Query Frame = 0

Query: 23  IDVREEYANAFRTESYIDFWTRV---LALNNGDDLTTQLSVESTTATHLSSYRLFVEHLL 82
           + V EEY  AFRT SY++  T+    L + +   L++  S   ++++ LS +  F ++LL
Sbjct: 34  LSVNEEYKEAFRTNSYLETRTKAEDQLGITSCSKLSSS-SPSPSSSSDLSFHSHFTDYLL 93

Query: 83  DPPQSTIKRILTSPHLGANSCSLLLDYFSHTANASLSCSHLLK-----HINHLRLKLHPL 142
           DPPQ T+  ++    L     +L++ +F  ++ A   C  LL+      INH ++K   +
Sbjct: 94  DPPQETLDALMQDSSLD----NLIVTFFDLSSEACDVCETLLQCLQQIKINHNKIK-RVM 153

Query: 143 EITLQSLENKEKFHHESHFKQLLI--RLVEFSNTRNPFIPSIEQVQ--IIQNGCSKLLKQ 202
           +I  +     +           LI   L  F+  +NP    + + Q  I+ +  S LL +
Sbjct: 154 KIGKRVCNGAKTLECSPEMLCALIFQELSRFAALKNPLCRIVNEAQFRIVHDANSDLLTK 213

Query: 203 LEFSRDKAQTKLKRVRYFQHSSAGFLVAVTASLTVIIVTHGIALF-VAAPGFLVGAIKLA 262
           L   + + + K+             ++ V A+  ++    G+  F +       G +  +
Sbjct: 214 LTSKKRRIRRKIS------------ILGVFAAPALL----GLCSFCLLRKKKAKGRMHKS 273

Query: 263 NQLRKLVKDVARLNITAKGTYTLNRDFDTIGRLVARLNHELEHMRVMARFWLDRGEDKRR 322
           N+   L K   +++I AKG + L  D DT+ RL  RL  E+EH + +A   +     K  
Sbjct: 274 NKDTTLEKLGTQIDIAAKGMFILINDLDTLSRLAGRLCDEIEHRKTVAA--MCAKSRKIE 333

Query: 323 AIGELVRQLNQSHVNFSHQLDELEEHLYLCFMTINRARNLVVKEI 355
            + E +R+ N     FS QL ELEEHLYLCF TINR+R LV+ +I
Sbjct: 334 VLKEALREFNGHEEKFSDQLQELEEHLYLCFHTINRSRRLVLAQI 354

BLAST of Bhi12G000442 vs. ExPASy Swiss-Prot
Match: Q9SMU4 (UPF0496 protein At3g49070 OS=Arabidopsis thaliana OX=3702 GN=At3g49070 PE=2 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 2.4e-81
Identity = 174/350 (49.71%), Postives = 222/350 (63.43%), Query Frame = 0

Query: 22  DIDVREEYANAFRTESYIDFWTRVLALNNGDDLTTQLS-----VESTTATHLSSYRLFVE 81
           D+DVREEYANAFRTESY  FWTRV+ L+      +  S       S+T+  L SYRLF  
Sbjct: 68  DVDVREEYANAFRTESYNHFWTRVVQLSRKKSAVSSSSSSPPIESSSTSARLMSYRLFAH 127

Query: 82  HLLDPPQSTIKRILTSPHLGANSCSLLLDYFSHTANASLSCSHLLKHINHLRLKLHPLEI 141
           +LLDP  +TI RIL    +G ++ +LL DYF  TANA L C+ LLK+I+HLR K   L  
Sbjct: 128 NLLDPDLNTITRILDVSRVGRHTRTLLSDYFLETANAFLLCTQLLKNIHHLRSKYESL-- 187

Query: 142 TLQSLENKEKFHHESHFKQLLI-RLVEFSNTRNPFIPSIEQVQIIQNGCSKLLKQLEFSR 201
                  K KFH E+H    LI +  E S   +PFI S  ++Q+I++GC  LLK+LE  R
Sbjct: 188 -------KPKFHSENHNSLALIDQFTEISKWFDPFISSGSRIQLIRSGCLYLLKRLESRR 247

Query: 202 DKAQTKLKRVRYFQHSSAGFLVAVTASLTVIIVTHGIALFVAAPGFLVGAIKLANQLRKL 261
           DK + KLK +    HSS   ++A+T +L V I +H  ALF+AAP  L    K A    KL
Sbjct: 248 DKTRAKLKLINGLTHSSGLLVLALTTTLIVTIASHAFALFLAAPTLLASQFKPAGLRNKL 307

Query: 262 VKDVARLNITAKGTYTLNRDFDTIGRLVARLNHELEHMRVMARFWLDRGEDKRRAIGELV 321
            K  ARL++ AKGTY L+RD DTI RLV R+N E+ H+R MA FW+ RG  + R   E+ 
Sbjct: 308 TKTAARLDVAAKGTYILSRDLDTISRLVTRINDEVNHVRAMAEFWVGRGSGRVRGSEEVA 367

Query: 322 RQLNQSHVNFSHQLDELEEHLYLCFMTINRARNLVVKEILDSGQPIKISY 366
           R+L +   +FS +LDELEEH+YLCFMTINRARNL+VKEILDS  P   S+
Sbjct: 368 RELKRCEESFSEELDELEEHIYLCFMTINRARNLLVKEILDSDDPPNCSF 408

BLAST of Bhi12G000442 vs. ExPASy Swiss-Prot
Match: A2XCJ1 (UPF0496 protein 3 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_009784 PE=3 SV=2)

HSP 1 Score: 181.8 bits (460), Expect = 1.4e-44
Identity = 136/354 (38.42%), Postives = 188/354 (53.11%), Query Frame = 0

Query: 16  VTSHSVDIDVREEYANAFRTESYIDFWTRVL--ALNNGDDLTTQLSVESTTAT--HLSSY 75
           V + S   D REEY +AFRTESY DFW RVL   L +G  L  +       A    L SY
Sbjct: 25  VVAPSSSFDFREEYTSAFRTESYNDFWARVLDITLAHGAALVPRHGGGGGCAASKRLPSY 84

Query: 76  RLFVEHLLDPPQSTIKRILTSP---HLGANSCSLLLDYFSHTANASLSCSHLLKHINHLR 135
           RLF EHLL+P Q  +   L SP    L  +   LL  Y++ TANAS  CSHLLK I H+R
Sbjct: 85  RLFAEHLLEPDQRAVAAALASPRGSRLRPDVRGLLAAYYAETANASFLCSHLLKDIEHIR 144

Query: 136 LKLHPLEITLQSLENKEKFHHESHFKQLLIRLVEFSNTRNPFIPSIEQVQIIQNGCSKLL 195
           L+  PL+ TL+ L +      +     L                S  +++ +Q G   LL
Sbjct: 145 LRYRPLKHTLRKLAS------DVGVSGLADVSAALGQPFTALAASQGRLREVQAGSGDLL 204

Query: 196 KQLEFSRDKAQTKLKRVRYFQHS-SAGFL--VAVTASLTVIIVTHGIALFVAAPGFLVGA 255
           + L+  R KA+ +++ V   + + S  F+  VAV A +   I  H +A F A P  ++  
Sbjct: 205 RGLDAGRKKARHRIRSVARLRRALSVSFVTAVAVVAVVGACIGVHILAAFAAFP--MMSP 264

Query: 256 IKLANQL---RKLVKDVARLNITAKGTYTLNRDFDTIGRLVARLNHELEHMRVMARFWLD 315
             L  +    R   + + +L   AKGTY LNRD +TI RLVAR+  E EHM  + R  ++
Sbjct: 265 AWLGERFFSGRAARRALVQLEAAAKGTYILNRDMETISRLVARVRDEGEHMVALLRLCVE 324

Query: 316 R-----GEDKRRAIGELVRQLNQSHVNFSHQLDELEEHLYLCFMTINRARNLVV 352
                    K R + E++RQL+++  +F  QLDELEEHL+LCFMTIN+AR +V+
Sbjct: 325 HRPAAGAGGKGRLVQEVLRQLSKNEESFRQQLDELEEHLFLCFMTINKARIMVM 370

BLAST of Bhi12G000442 vs. ExPASy Swiss-Prot
Match: Q10RR9 (UPF0496 protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0148000 PE=2 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 5.3e-44
Identity = 135/354 (38.14%), Postives = 187/354 (52.82%), Query Frame = 0

Query: 16  VTSHSVDIDVREEYANAFRTESYIDFWTRVL--ALNNGDDLTTQLSVESTTAT--HLSSY 75
           V + S   D REEY +AFRTESY DFW RVL   L +G  L  +       A    L SY
Sbjct: 25  VVAPSSSFDFREEYTSAFRTESYNDFWARVLDITLAHGAALVPRHGGGGGCAASKRLPSY 84

Query: 76  RLFVEHLLDPPQSTIKRILTSP---HLGANSCSLLLDYFSHTANASLSCSHLLKHINHLR 135
           RLF EHLL+P Q  +   L SP    L  +   LL  Y++ TANAS  CSHLLK I H+R
Sbjct: 85  RLFAEHLLEPDQRAVAAALASPRGSRLRPDVRGLLAAYYAETANASFLCSHLLKDIEHIR 144

Query: 136 LKLHPLEITLQSLENKEKFHHESHFKQLLIRLVEFSNTRNPFIPSIEQVQIIQNGCSKLL 195
           L+  PL+ TL+ L +      +     L                S  +++ +Q G   LL
Sbjct: 145 LRYRPLKHTLRKLAS------DVGVSGLADVSAALGQPFTALAASQGRLREVQAGSGDLL 204

Query: 196 KQLEFSRDKAQTKLKRVRYFQHS-SAGFL--VAVTASLTVIIVTHGIALFVAAPGFLVGA 255
           + L+  R KA+ +++ V   + + S  F+  VAV A +   I  H +A F A P  ++  
Sbjct: 205 RGLDAGRKKARHRIRSVARLRRALSVSFVTAVAVVAVVGACIGVHILAAFAAFP--MMSP 264

Query: 256 IKLANQL---RKLVKDVARLNITAKGTYTLNRDFDTIGRLVARLNHELEHMRVMARFWLD 315
             L  +    R   + + +L   AKGTY LNRD +TI RLVAR+  E EHM  + R  ++
Sbjct: 265 AWLGERFFSGRAARRALVQLEAAAKGTYILNRDMETISRLVARVRDEGEHMVALRRLCVE 324

Query: 316 R-----GEDKRRAIGELVRQLNQSHVNFSHQLDELEEHLYLCFMTINRARNLVV 352
                    K R + E++RQL+++  +F  QLDELEEHL+LCFMT N+AR +V+
Sbjct: 325 HRPAAGAGGKGRLVQEVLRQLSKNEESFRQQLDELEEHLFLCFMTTNKARIMVM 370

BLAST of Bhi12G000442 vs. ExPASy Swiss-Prot
Match: Q9LJK4 (UPF0496 protein At3g19250 OS=Arabidopsis thaliana OX=3702 GN=At3g19250 PE=2 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 2.2e-26
Identity = 103/362 (28.45%), Postives = 184/362 (50.83%), Query Frame = 0

Query: 7   SIQTDGEPHVT---SHSVDIDVREEYANAFRTESYIDFWTRVLALNNGDDLTTQLSVEST 66
           S+  D  PH +   S +   ++  E A+AF+T SY D  +R+L ++      TQ ++E  
Sbjct: 15  SLGDDHLPHPSPEGSVASTFNLSHELAHAFQTPSYHDIRSRLLVID-----PTQENLE-- 74

Query: 67  TATHLSSYRLFVEHLLDPPQSTIKRILTSPHLGANS-CSLLLDYFSHTANASLSCSHLLK 126
                    LF+   L P   +++  L+  H    +  +L+  YF H+ +A+  C +L +
Sbjct: 75  ---------LFLSQELRPNNESVQEALSLRHAKQTTLTNLVSTYFQHSEDATRFCLNLYQ 134

Query: 127 HINHLRLKLHPLEITLQSLENKEKFH--HESHFKQLLIRLVEFSNTRNPFI-PSIEQVQI 186
           +++  R  L+   + L ++  ++      ES         ++     NPF  P     Q 
Sbjct: 135 NVHSARCHLYTPLLDLFNIFPRDSHSAIDESFCNLAFDVFLKLDTFENPFASPESHSFQD 194

Query: 187 IQNGCSKLLKQLEFSRDKAQTKLKRVRYFQHSSAGFLVAVTASLTVIIVTHGIALFVAAP 246
            Q    +L  +L+    K+++   RVR   H++AG  + +  ++ V+  +     + A P
Sbjct: 195 TQLCFYQLADKLDTRIRKSKS---RVRLLHHATAGSALCLVTAVVVVAASAAFIAYHALP 254

Query: 247 GFLVGAIKLANQL------RKLVKDVARLNITAKGTYTLNRDFDTIGRLVARLNHELEHM 306
             LV A  L          +K + ++ +LN+ AKGT+ LN+D DTI RLV+RL+  +++ 
Sbjct: 255 TILVVAGPLCTPYLPHSFKKKELSNIFQLNVAAKGTFALNKDLDTIDRLVSRLHTGVKND 314

Query: 307 RVMARFWLDRGEDKRRAIGELVRQLNQSHVNFSHQLDELEEHLYLCFMTINRARNLVVKE 356
           +++ R  L+RG D    I E V+QL +SHVN +HQL+ L +H+   F  +N++R+L++KE
Sbjct: 315 KLLIRLGLERGRDV-YTIPEFVKQLRKSHVNHTHQLEVLVDHICRWFTNVNKSRSLLLKE 356

BLAST of Bhi12G000442 vs. ExPASy Swiss-Prot
Match: Q6DYE5 (UPF0496 protein At1g20180 OS=Arabidopsis thaliana OX=3702 GN=At1g20180 PE=2 SV=2)

HSP 1 Score: 119.4 bits (298), Expect = 8.5e-26
Identity = 108/361 (29.92%), Postives = 173/361 (47.92%), Query Frame = 0

Query: 23  IDVREEYANAFRTESYIDFWTRV---LALNNGDDLTTQLSVESTTATHLSSYRLFVEHLL 82
           + V EEY  AFRT SY++  T+    L + +   L++  S   ++++ LS +  F ++LL
Sbjct: 34  LSVNEEYKEAFRTNSYLETRTKAEDQLGITSCSKLSSS-SPSPSSSSDLSFHSHFTDYLL 93

Query: 83  DPPQSTIKRILTSPHLGANSCSLLLDYFSHTANASLSCSHLLK-----HINHLRLKLHPL 142
           DPPQ T+  ++    L     +L++ +F  ++ A   C  LL+      INH ++K   +
Sbjct: 94  DPPQETLDALMQDSSLD----NLIVTFFDLSSEACDVCETLLQCLQQIKINHNKIK-RVM 153

Query: 143 EITLQSLENKEKFHHESHFKQLLI--RLVEFSNTRNPFIPSIEQVQ--IIQNGCSKLLKQ 202
           +I  +     +           LI   L  F+  +NP    + + Q  I+ +  S LL +
Sbjct: 154 KIGKRVCNGAKTLECSPEMLCALIFQELSRFAALKNPLCRIVNEAQFRIVHDANSDLLTK 213

Query: 203 LEFSRDKAQTKLKRVRYFQHSSAGFLVAVTASLTV----IIVTHGIALFVAAPGFL---- 262
           L   + + + K++  + F     G+ + +T S  V    II  H I    AAP  L    
Sbjct: 214 LTSKKRRIRRKIRFFK-FCKKLGGYSLVITHSAIVITLLIIALHSILGVFAAPALLGLCS 273

Query: 263 ---------VGAIKLANQLRKLVKDVARLNITAKGTYTLNRDFDTIGRLVARLNHELEHM 322
                     G +  +N+   L K   +++I AKG + L  D DT+ RL  RL  E+EH 
Sbjct: 274 FCLLRKKKAKGRMHKSNKDTTLEKLGTQIDIAAKGMFILINDLDTLSRLAGRLCDEIEHR 333

Query: 323 RVMARFWLDRGEDKRRAIGELVRQLNQSHVNFSHQLDELEEHLYLCFMTINRARNLVVKE 355
           + +A   +     K   + E +R+ N     FS QL ELEEHLYLCF TINR+R LV+ +
Sbjct: 334 KTVAA--MCAKSRKIEVLKEALREFNGHEEKFSDQLQELEEHLYLCFHTINRSRRLVLAQ 385

BLAST of Bhi12G000442 vs. NCBI nr
Match: XP_038875828.1 (UPF0496 protein At3g49070-like [Benincasa hispida])

HSP 1 Score: 695.3 bits (1793), Expect = 2.9e-196
Identity = 356/357 (99.72%), Postives = 357/357 (100.00%), Query Frame = 0

Query: 10  TDGEPHVTSHSVDIDVREEYANAFRTESYIDFWTRVLALNNGDDLTTQLSVESTTATHLS 69
           +DGEPHVTSHSVDIDVREEYANAFRTESYIDFWTRVLALNNGDDLTTQLSVESTTATHLS
Sbjct: 17  SDGEPHVTSHSVDIDVREEYANAFRTESYIDFWTRVLALNNGDDLTTQLSVESTTATHLS 76

Query: 70  SYRLFVEHLLDPPQSTIKRILTSPHLGANSCSLLLDYFSHTANASLSCSHLLKHINHLRL 129
           SYRLFVEHLLDPPQSTIKRILTSPHLGANSCSLLLDYFSHTANASLSCSHLLKHINHLRL
Sbjct: 77  SYRLFVEHLLDPPQSTIKRILTSPHLGANSCSLLLDYFSHTANASLSCSHLLKHINHLRL 136

Query: 130 KLHPLEITLQSLENKEKFHHESHFKQLLIRLVEFSNTRNPFIPSIEQVQIIQNGCSKLLK 189
           KLHPLEITLQSLENKEKFHHESHFKQLLIRLVEFSNTRNPFIPSIEQVQIIQNGCSKLLK
Sbjct: 137 KLHPLEITLQSLENKEKFHHESHFKQLLIRLVEFSNTRNPFIPSIEQVQIIQNGCSKLLK 196

Query: 190 QLEFSRDKAQTKLKRVRYFQHSSAGFLVAVTASLTVIIVTHGIALFVAAPGFLVGAIKLA 249
           QLEFSRDKAQTKLKRVRYFQHSSAGFLVAVTASLTVIIVTHGIALFVAAPGFLVGAIKLA
Sbjct: 197 QLEFSRDKAQTKLKRVRYFQHSSAGFLVAVTASLTVIIVTHGIALFVAAPGFLVGAIKLA 256

Query: 250 NQLRKLVKDVARLNITAKGTYTLNRDFDTIGRLVARLNHELEHMRVMARFWLDRGEDKRR 309
           NQLRKLVKDVARLNITAKGTYTLNRDFDTIGRLVARLNHELEHMRVMARFWLDRGEDKRR
Sbjct: 257 NQLRKLVKDVARLNITAKGTYTLNRDFDTIGRLVARLNHELEHMRVMARFWLDRGEDKRR 316

Query: 310 AIGELVRQLNQSHVNFSHQLDELEEHLYLCFMTINRARNLVVKEILDSGQPIKISYL 367
           AIGELVRQLNQSHVNFSHQLDELEEHLYLCFMTINRARNLVVKEILDSGQPIKISYL
Sbjct: 317 AIGELVRQLNQSHVNFSHQLDELEEHLYLCFMTINRARNLVVKEILDSGQPIKISYL 373

BLAST of Bhi12G000442 vs. NCBI nr
Match: XP_008436996.1 (PREDICTED: UPF0496 protein At3g49070 isoform X1 [Cucumis melo])

HSP 1 Score: 538.5 bits (1386), Expect = 4.5e-149
Identity = 286/362 (79.01%), Postives = 319/362 (88.12%), Query Frame = 0

Query: 10  TDGEPHVTSHSVDIDVREEYANAFRTESYIDFWTRVLALNNGDDLTTQLSVESTTATHLS 69
           +DGE HVT++ VD DV EEYANAFRTESYIDFWTRV+ALNNGD+LT Q+S+ESTTAT LS
Sbjct: 17  SDGEQHVTNYPVDTDVGEEYANAFRTESYIDFWTRVVALNNGDNLTAQVSLESTTATRLS 76

Query: 70  SYRLFVEHLLDPPQSTIKRILTSPHLGANSCSLLLD-YFSHTANASLSCSHLLKHINHLR 129
           SYRLFVEHLLDPPQ TIKR+LT+ HLG NSCSLLLD YFSHT+NASL CS +LKHI HLR
Sbjct: 77  SYRLFVEHLLDPPQPTIKRMLTA-HLGPNSCSLLLDHYFSHTSNASLLCSRILKHIVHLR 136

Query: 130 LKLHPLEITLQSLENKEKFHH-ESHFKQLLIRLVEFSN--TRNPFIP-SIEQVQIIQNGC 189
           LKL  L+      +NK++F++ +SHFKQLL+ LVEFSN  T N F+P  +EQVQIIQNGC
Sbjct: 137 LKLRSLD------QNKQEFNYDDSHFKQLLVCLVEFSNNSTPNSFVPYCMEQVQIIQNGC 196

Query: 190 SKLLKQLEFSRDKAQTKLKRVRYFQHSSAGFLVAVTASLTVIIVTHGIALFVAAPGFLVG 249
           SKLLK+LE+SRDKAQ KLKRVRYFQHSSAGFLVA+TAS T+I+VTHGIALFVAAPGFLVG
Sbjct: 197 SKLLKRLEYSRDKAQDKLKRVRYFQHSSAGFLVAITASFTIIVVTHGIALFVAAPGFLVG 256

Query: 250 AIKLANQLRKLVKDVARLNITAKGTYTLNRDFDTIGRLVARLNHELEHMRVMARFWLDRG 309
           AIKL  + RKL K VA+LN+ AKGTYTLNRDFDTIGRLVARL+HELEHMRVM +FWLDRG
Sbjct: 257 AIKLVKKSRKLAKKVAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEHMRVMTKFWLDRG 316

Query: 310 EDKRRAIGELVRQLNQSHVNFSHQLDELEEHLYLCFMTINRARNLVVKEILDSGQPIKIS 367
           EDKR AIGEL+RQLNQSH NF+ QLDELEEHLYLCFMTINRARNLVVKEILDS  PIKIS
Sbjct: 317 EDKRWAIGELLRQLNQSHENFNQQLDELEEHLYLCFMTINRARNLVVKEILDSSAPIKIS 371

BLAST of Bhi12G000442 vs. NCBI nr
Match: XP_031741820.1 (UPF0496 protein At3g49070 [Cucumis sativus] >KAE8647914.1 hypothetical protein Csa_000030 [Cucumis sativus])

HSP 1 Score: 526.9 bits (1356), Expect = 1.4e-145
Identity = 281/362 (77.62%), Postives = 315/362 (87.02%), Query Frame = 0

Query: 10  TDGEPHVTSHSVDIDVREEYANAFRTESYIDFWTRVLALNNGDDLTTQLSVESTTATHLS 69
           +DG  HV ++ VD DV EEYANAFRTESYIDFWTRV+ALNNGD+LT Q+S+ESTTAT LS
Sbjct: 17  SDGGQHVINYPVDTDVGEEYANAFRTESYIDFWTRVVALNNGDNLTAQVSLESTTATRLS 76

Query: 70  SYRLFVEHLLDPPQSTIKRILTSPHLGANSCSLLLD-YFSHTANASLSCSHLLKHINHLR 129
           SYRLFVEHLLDPPQ TIK ILT+ HLG NSCSLLLD YFSHTANASL CS +LK I HLR
Sbjct: 77  SYRLFVEHLLDPPQPTIKTILTA-HLGPNSCSLLLDHYFSHTANASLLCSRILKQIVHLR 136

Query: 130 LKLHPLEITLQSLENKEKFHH-ESHFKQLLIRLVEFSN--TRNPFIP-SIEQVQIIQNGC 189
           LKLH L+      +NK++F+H +SHFKQLL+RL EFSN  T N F+P  +EQVQIIQNGC
Sbjct: 137 LKLHSLD------QNKQEFNHDDSHFKQLLVRLFEFSNDSTPNSFVPYCMEQVQIIQNGC 196

Query: 190 SKLLKQLEFSRDKAQTKLKRVRYFQHSSAGFLVAVTASLTVIIVTHGIALFVAAPGFLVG 249
           SKLLK+LE+SRDK + KLKRVRYFQHSSAGFLVA+TAS TVI+VTHGIALFVAAPGFLVG
Sbjct: 197 SKLLKRLEYSRDKTRDKLKRVRYFQHSSAGFLVAITASFTVIVVTHGIALFVAAPGFLVG 256

Query: 250 AIKLANQLRKLVKDVARLNITAKGTYTLNRDFDTIGRLVARLNHELEHMRVMARFWLDRG 309
           AIKLA + RKL K+VA+LN+ AKGTYTLNRDFDTIGRLVARL+HELEHM+VMA+FWLD+G
Sbjct: 257 AIKLAKKSRKLAKEVAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEHMKVMAKFWLDKG 316

Query: 310 EDKRRAIGELVRQLNQSHVNFSHQLDELEEHLYLCFMTINRARNLVVKEILDSGQPIKIS 367
            DKR AI EL RQLNQSH NF+ QLDELEEHLYLCFMTINRARNLVVKEIL+  +PIKIS
Sbjct: 317 GDKRWAIDELARQLNQSHENFNQQLDELEEHLYLCFMTINRARNLVVKEILNLSKPIKIS 371

BLAST of Bhi12G000442 vs. NCBI nr
Match: XP_022159588.1 (UPF0496 protein At3g49070 [Momordica charantia])

HSP 1 Score: 500.0 bits (1286), Expect = 1.8e-137
Identity = 259/360 (71.94%), Postives = 302/360 (83.89%), Query Frame = 0

Query: 10  TDGEPHVTSHSVDIDVREEYANAFRTESYIDFWTRVLALNNGDDLTTQLSVESTTATHLS 69
           +DGEPH+TS+ + +DVREEYANAFRTESYIDFWTRVL LN+G DLTTQL VESTTAT LS
Sbjct: 17  SDGEPHITSYPIGVDVREEYANAFRTESYIDFWTRVLTLNDG-DLTTQLPVESTTATRLS 76

Query: 70  SYRLFVEHLLDPPQSTIKRILTSPHLGANSCSLLLDYFSHTANASLSCSHLLKHINHLRL 129
           SYRLF EHLLDP Q T+KR+L S +L  NS SLL DYFSHTANASL C  LLK I++LRL
Sbjct: 77  SYRLFAEHLLDPTQPTVKRLLASTYLRPNSYSLLTDYFSHTANASLLCGRLLKDIHNLRL 136

Query: 130 KLHPLEITLQSLENKEKFHHESHFKQLLIRLVEFSNTRNPFI---PSIEQVQIIQNGCSK 189
           KL PL+ITL S+EN  +F+H  +F+ +L  LVEFSN  NPF+   PS  +++IIQ+GCS 
Sbjct: 137 KLRPLKITLHSIEN-TRFYHNYNFEPILTCLVEFSNAHNPFVSSAPSTRRIRIIQSGCSN 196

Query: 190 LLKQLEFSRDKAQTKLKRVRYFQHSSAGFLVAVTASLTVIIVTHGIALFVAAPGFLVGAI 249
           LLKQLEF+RDKA+ KL+RVRYFQH SAGFLVA+T SLTVI++THGIALFVAAPG LV +I
Sbjct: 197 LLKQLEFNRDKARAKLRRVRYFQHGSAGFLVALTTSLTVIVMTHGIALFVAAPGLLVASI 256

Query: 250 KLANQLRKLVKDVARLNITAKGTYTLNRDFDTIGRLVARLNHELEHMRVMARFWLDRGED 309
           +LA   RKL K VA+LN+ AKGTYTLNRDFDT+GRLVARL+HELEHMRVM RFWL+R ED
Sbjct: 257 ELAKS-RKLAKQVAQLNVAAKGTYTLNRDFDTVGRLVARLSHELEHMRVMTRFWLERRED 316

Query: 310 KRRAIGELVRQLNQSHVNFSHQLDELEEHLYLCFMTINRARNLVVKEILDSGQPIKISYL 367
           +R+AI E+ RQL Q+H +FS QLDELEEHLYLCFMTINRARNLVVKEILD GQP K  ++
Sbjct: 317 RRQAISEVARQLKQNHASFSQQLDELEEHLYLCFMTINRARNLVVKEILDPGQPAKAPHV 373

BLAST of Bhi12G000442 vs. NCBI nr
Match: XP_023534108.1 (UPF0496 protein At3g49070-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 374.4 bits (960), Expect = 1.1e-99
Identity = 220/354 (62.15%), Postives = 257/354 (72.60%), Query Frame = 0

Query: 10  TDGEPHVTSHSVDIDVREEYANAFRTESYIDFWTRVLALNNGDDLTTQLSVESTTATHLS 69
           +DG  H+ S  + +DVREEYANAFRTESY+DFWTRV+A+ +       + VESTTA+ LS
Sbjct: 15  SDGGVHIRSRPIGVDVREEYANAFRTESYLDFWTRVVAIKD-------VVVESTTASRLS 74

Query: 70  SYRLFVEHLLDPPQSTIKRILTSPHLGANSCSLLLDYFSHTANASLSCSHLLKHINHLRL 129
           SYRLF EHLLDP + T+KRIL   HL  NS SLL DYFSHTANASL CS LLK INHLR 
Sbjct: 75  SYRLFAEHLLDPTEPTVKRILNWAHLRPNSKSLLSDYFSHTANASLLCSRLLKDINHLR- 134

Query: 130 KLHPLEITLQSLENKEKFHHESHFKQLLIRLVEFSNTRNPFIPSIEQVQIIQNGCSKLLK 189
                EI +QSL+N      E +FK L I         NPF       +IIQNGC+KLLK
Sbjct: 135 ----PEIAIQSLQNP-----EFNFKPLSI--------HNPF------PEIIQNGCAKLLK 194

Query: 190 QLEFSRDKAQTKLKRVRYFQHSSAGFLVAVTASLTVIIVTHGIALFV--AAPGF--LVGA 249
           QLEF+RDKA+TK+KRVRYFQHSSAG LVAVTASLTVI+VTHGI L V  AAPG   LVGA
Sbjct: 195 QLEFNRDKARTKVKRVRYFQHSSAGLLVAVTASLTVILVTHGIGLVVVAAAPGLAGLVGA 254

Query: 250 IKLANQLRKLVKDVAR-LNITAKGTYTLNRDFDTIGRLVARLNHELEHMRVMARFWLDRG 309
           +KLA      VK++AR L++ AK TYTLNRDFDT+GRLVARLN E+EHMR + RFW++ G
Sbjct: 255 VKLAR-----VKEMARVLDVAAKATYTLNRDFDTVGRLVARLNQEVEHMRGLMRFWVELG 314

Query: 310 E---DKRRAIGELVRQLNQSHVNFSHQLDELEEHLYLCFMTINRARNLVVKEIL 356
           E    +   +GE+ RQL Q  +N    LDELEEHLYLCFMTINRARNLV+K+IL
Sbjct: 315 EGRDGRHGGVGEVARQLQQCRLNLGQHLDELEEHLYLCFMTINRARNLVLKQIL 332

BLAST of Bhi12G000442 vs. ExPASy TrEMBL
Match: A0A1S3ATJ2 (UPF0496 protein At3g49070 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482559 PE=3 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 2.2e-149
Identity = 286/362 (79.01%), Postives = 319/362 (88.12%), Query Frame = 0

Query: 10  TDGEPHVTSHSVDIDVREEYANAFRTESYIDFWTRVLALNNGDDLTTQLSVESTTATHLS 69
           +DGE HVT++ VD DV EEYANAFRTESYIDFWTRV+ALNNGD+LT Q+S+ESTTAT LS
Sbjct: 17  SDGEQHVTNYPVDTDVGEEYANAFRTESYIDFWTRVVALNNGDNLTAQVSLESTTATRLS 76

Query: 70  SYRLFVEHLLDPPQSTIKRILTSPHLGANSCSLLLD-YFSHTANASLSCSHLLKHINHLR 129
           SYRLFVEHLLDPPQ TIKR+LT+ HLG NSCSLLLD YFSHT+NASL CS +LKHI HLR
Sbjct: 77  SYRLFVEHLLDPPQPTIKRMLTA-HLGPNSCSLLLDHYFSHTSNASLLCSRILKHIVHLR 136

Query: 130 LKLHPLEITLQSLENKEKFHH-ESHFKQLLIRLVEFSN--TRNPFIP-SIEQVQIIQNGC 189
           LKL  L+      +NK++F++ +SHFKQLL+ LVEFSN  T N F+P  +EQVQIIQNGC
Sbjct: 137 LKLRSLD------QNKQEFNYDDSHFKQLLVCLVEFSNNSTPNSFVPYCMEQVQIIQNGC 196

Query: 190 SKLLKQLEFSRDKAQTKLKRVRYFQHSSAGFLVAVTASLTVIIVTHGIALFVAAPGFLVG 249
           SKLLK+LE+SRDKAQ KLKRVRYFQHSSAGFLVA+TAS T+I+VTHGIALFVAAPGFLVG
Sbjct: 197 SKLLKRLEYSRDKAQDKLKRVRYFQHSSAGFLVAITASFTIIVVTHGIALFVAAPGFLVG 256

Query: 250 AIKLANQLRKLVKDVARLNITAKGTYTLNRDFDTIGRLVARLNHELEHMRVMARFWLDRG 309
           AIKL  + RKL K VA+LN+ AKGTYTLNRDFDTIGRLVARL+HELEHMRVM +FWLDRG
Sbjct: 257 AIKLVKKSRKLAKKVAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEHMRVMTKFWLDRG 316

Query: 310 EDKRRAIGELVRQLNQSHVNFSHQLDELEEHLYLCFMTINRARNLVVKEILDSGQPIKIS 367
           EDKR AIGEL+RQLNQSH NF+ QLDELEEHLYLCFMTINRARNLVVKEILDS  PIKIS
Sbjct: 317 EDKRWAIGELLRQLNQSHENFNQQLDELEEHLYLCFMTINRARNLVVKEILDSSAPIKIS 371

BLAST of Bhi12G000442 vs. ExPASy TrEMBL
Match: A0A0A0KN20 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G166480 PE=3 SV=1)

HSP 1 Score: 524.2 bits (1349), Expect = 4.2e-145
Identity = 280/360 (77.78%), Postives = 313/360 (86.94%), Query Frame = 0

Query: 12  GEPHVTSHSVDIDVREEYANAFRTESYIDFWTRVLALNNGDDLTTQLSVESTTATHLSSY 71
           G  HV ++ VD DV EEYANAFRTESYIDFWTRV+ALNNGD+LT Q+S+ESTTAT LSSY
Sbjct: 4   GGQHVINYPVDTDVGEEYANAFRTESYIDFWTRVVALNNGDNLTAQVSLESTTATRLSSY 63

Query: 72  RLFVEHLLDPPQSTIKRILTSPHLGANSCSLLLD-YFSHTANASLSCSHLLKHINHLRLK 131
           RLFVEHLLDPPQ TIK ILT+ HLG NSCSLLLD YFSHTANASL CS +LK I HLRLK
Sbjct: 64  RLFVEHLLDPPQPTIKTILTA-HLGPNSCSLLLDHYFSHTANASLLCSRILKQIVHLRLK 123

Query: 132 LHPLEITLQSLENKEKFHH-ESHFKQLLIRLVEFSN--TRNPFIP-SIEQVQIIQNGCSK 191
           LH L+      +NK++F+H +SHFKQLL+RL EFSN  T N F+P  +EQVQIIQNGCSK
Sbjct: 124 LHSLD------QNKQEFNHDDSHFKQLLVRLFEFSNDSTPNSFVPYCMEQVQIIQNGCSK 183

Query: 192 LLKQLEFSRDKAQTKLKRVRYFQHSSAGFLVAVTASLTVIIVTHGIALFVAAPGFLVGAI 251
           LLK+LE+SRDK + KLKRVRYFQHSSAGFLVA+TAS TVI+VTHGIALFVAAPGFLVGAI
Sbjct: 184 LLKRLEYSRDKTRDKLKRVRYFQHSSAGFLVAITASFTVIVVTHGIALFVAAPGFLVGAI 243

Query: 252 KLANQLRKLVKDVARLNITAKGTYTLNRDFDTIGRLVARLNHELEHMRVMARFWLDRGED 311
           KLA + RKL K+VA+LN+ AKGTYTLNRDFDTIGRLVARL+HELEHM+VMA+FWLD+G D
Sbjct: 244 KLAKKSRKLAKEVAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEHMKVMAKFWLDKGGD 303

Query: 312 KRRAIGELVRQLNQSHVNFSHQLDELEEHLYLCFMTINRARNLVVKEILDSGQPIKISYL 367
           KR AI EL RQLNQSH NF+ QLDELEEHLYLCFMTINRARNLVVKEIL+  +PIKISYL
Sbjct: 304 KRWAIDELARQLNQSHENFNQQLDELEEHLYLCFMTINRARNLVVKEILNLSKPIKISYL 356

BLAST of Bhi12G000442 vs. ExPASy TrEMBL
Match: A0A6J1DZ81 (UPF0496 protein At3g49070 OS=Momordica charantia OX=3673 GN=LOC111025959 PE=3 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 8.6e-138
Identity = 259/360 (71.94%), Postives = 302/360 (83.89%), Query Frame = 0

Query: 10  TDGEPHVTSHSVDIDVREEYANAFRTESYIDFWTRVLALNNGDDLTTQLSVESTTATHLS 69
           +DGEPH+TS+ + +DVREEYANAFRTESYIDFWTRVL LN+G DLTTQL VESTTAT LS
Sbjct: 17  SDGEPHITSYPIGVDVREEYANAFRTESYIDFWTRVLTLNDG-DLTTQLPVESTTATRLS 76

Query: 70  SYRLFVEHLLDPPQSTIKRILTSPHLGANSCSLLLDYFSHTANASLSCSHLLKHINHLRL 129
           SYRLF EHLLDP Q T+KR+L S +L  NS SLL DYFSHTANASL C  LLK I++LRL
Sbjct: 77  SYRLFAEHLLDPTQPTVKRLLASTYLRPNSYSLLTDYFSHTANASLLCGRLLKDIHNLRL 136

Query: 130 KLHPLEITLQSLENKEKFHHESHFKQLLIRLVEFSNTRNPFI---PSIEQVQIIQNGCSK 189
           KL PL+ITL S+EN  +F+H  +F+ +L  LVEFSN  NPF+   PS  +++IIQ+GCS 
Sbjct: 137 KLRPLKITLHSIEN-TRFYHNYNFEPILTCLVEFSNAHNPFVSSAPSTRRIRIIQSGCSN 196

Query: 190 LLKQLEFSRDKAQTKLKRVRYFQHSSAGFLVAVTASLTVIIVTHGIALFVAAPGFLVGAI 249
           LLKQLEF+RDKA+ KL+RVRYFQH SAGFLVA+T SLTVI++THGIALFVAAPG LV +I
Sbjct: 197 LLKQLEFNRDKARAKLRRVRYFQHGSAGFLVALTTSLTVIVMTHGIALFVAAPGLLVASI 256

Query: 250 KLANQLRKLVKDVARLNITAKGTYTLNRDFDTIGRLVARLNHELEHMRVMARFWLDRGED 309
           +LA   RKL K VA+LN+ AKGTYTLNRDFDT+GRLVARL+HELEHMRVM RFWL+R ED
Sbjct: 257 ELAKS-RKLAKQVAQLNVAAKGTYTLNRDFDTVGRLVARLSHELEHMRVMTRFWLERRED 316

Query: 310 KRRAIGELVRQLNQSHVNFSHQLDELEEHLYLCFMTINRARNLVVKEILDSGQPIKISYL 367
           +R+AI E+ RQL Q+H +FS QLDELEEHLYLCFMTINRARNLVVKEILD GQP K  ++
Sbjct: 317 RRQAISEVARQLKQNHASFSQQLDELEEHLYLCFMTINRARNLVVKEILDPGQPAKAPHV 373

BLAST of Bhi12G000442 vs. ExPASy TrEMBL
Match: A0A6J1H2B8 (UPF0496 protein At3g49070-like OS=Cucurbita moschata OX=3662 GN=LOC111459391 PE=3 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 1.2e-99
Identity = 220/354 (62.15%), Postives = 256/354 (72.32%), Query Frame = 0

Query: 10  TDGEPHVTSHSVDIDVREEYANAFRTESYIDFWTRVLALNNGDDLTTQLSVESTTATHLS 69
           +DG  H+ S  + +DVREEYANAFRTESY+DFWTRV+AL +       + VESTTA+ LS
Sbjct: 15  SDGGVHIRSRPIGVDVREEYANAFRTESYLDFWTRVVALKD-------VVVESTTASRLS 74

Query: 70  SYRLFVEHLLDPPQSTIKRILTSPHLGANSCSLLLDYFSHTANASLSCSHLLKHINHLRL 129
           SYRLF EHLLDP + T+KRIL   HL  N  SLL DYFSHTANASL CS LLK +NHLR 
Sbjct: 75  SYRLFAEHLLDPTEPTVKRILNWAHLRPNFKSLLSDYFSHTANASLLCSRLLKDMNHLR- 134

Query: 130 KLHPLEITLQSLENKEKFHHESHFKQLLIRLVEFSNTRNPFIPSIEQVQIIQNGCSKLLK 189
                EI +QSL+N      E +FK L I         NPF        IIQNGC+KLLK
Sbjct: 135 ----PEIAIQSLQNP-----EFNFKPLSI--------HNPF------PAIIQNGCAKLLK 194

Query: 190 QLEFSRDKAQTKLKRVRYFQHSSAGFLVAVTASLTVIIVTHGIALFV--AAPGF--LVGA 249
           QLEF+RDKA+TK+KRVRYFQHSSAG LVAVTASLTVI+VTHGI L V  AAPG   LVGA
Sbjct: 195 QLEFNRDKARTKVKRVRYFQHSSAGLLVAVTASLTVILVTHGIGLVVVAAAPGLVGLVGA 254

Query: 250 IKLANQLRKLVKDVAR-LNITAKGTYTLNRDFDTIGRLVARLNHELEHMRVMARFWLDRG 309
           +KLA      VK+VAR L++ AK TYTLNRDFDT+GRLVARLN E+EHM+ + RFW++RG
Sbjct: 255 VKLAR-----VKEVARVLDVAAKATYTLNRDFDTVGRLVARLNQEVEHMKGLMRFWVERG 314

Query: 310 E---DKRRAIGELVRQLNQSHVNFSHQLDELEEHLYLCFMTINRARNLVVKEIL 356
           E    +   +GE+ RQL Q  +N    LDELEEHLYLCFMTINRARNLV+K+IL
Sbjct: 315 EGRDGRHGGVGEVARQLQQCRLNLGQHLDELEEHLYLCFMTINRARNLVLKQIL 332

BLAST of Bhi12G000442 vs. ExPASy TrEMBL
Match: A0A6J1JZN7 (UPF0496 protein At3g49070 OS=Cucurbita maxima OX=3661 GN=LOC111491151 PE=3 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 7.8e-99
Identity = 220/354 (62.15%), Postives = 256/354 (72.32%), Query Frame = 0

Query: 10  TDGEPHVTSHSVDIDVREEYANAFRTESYIDFWTRVLALNNGDDLTTQLSVESTTATHLS 69
           +DG  H+ S  + +DVREEYANAFRTESY+DFWTRV+AL +       ++VESTTA+ LS
Sbjct: 17  SDGGVHIRSRPIGVDVREEYANAFRTESYLDFWTRVVALKD-------VAVESTTASRLS 76

Query: 70  SYRLFVEHLLDPPQSTIKRILTSPHLGANSCSLLLDYFSHTANASLSCSHLLKHINHLRL 129
           SYRLF EHLLDP + T+KRIL   HL  NS SLL DYFSHTANASL CS LLK INHLR 
Sbjct: 77  SYRLFAEHLLDPNEPTVKRILNWAHLRPNSKSLLSDYFSHTANASLLCSRLLKDINHLR- 136

Query: 130 KLHPLEITLQSLENKEKFHHESHFKQLLIRLVEFSNTRNPFIPSIEQVQIIQNGCSKLLK 189
                EI +QSL+N      E +FK L I         NPF       +IIQNGC+KLLK
Sbjct: 137 ----PEIAIQSLQNP-----EFNFKPLSI--------HNPF------PEIIQNGCAKLLK 196

Query: 190 QLEFSRDKAQTKLKRVRYFQHSSAGFLVAVTASLTVIIVTHGIALFV--AAP--GFLVGA 249
           QLEF+RDKA+TK+KRVRYFQH SAG LVAVTASLTVI+VTHGI L V  AAP    LVGA
Sbjct: 197 QLEFNRDKARTKVKRVRYFQHGSAGLLVAVTASLTVILVTHGIGLVVVAAAPCLAGLVGA 256

Query: 250 IKLANQLRKLVKDVAR-LNITAKGTYTLNRDFDTIGRLVARLNHELEHMRVMARFWLDRG 309
           +KLA      VK+VAR L++ AK TYTLNRDFDT+GRLVARLN E+EHMR + RFW++RG
Sbjct: 257 VKLAR-----VKEVARVLDVAAKATYTLNRDFDTVGRLVARLNQEVEHMRGLMRFWVERG 316

Query: 310 E---DKRRAIGELVRQLNQSHVNFSHQLDELEEHLYLCFMTINRARNLVVKEIL 356
           E    +   +GE+ RQL Q  +N    LDELEE LYLCFMTINRARNLV+K+IL
Sbjct: 317 EGRDGRHGGVGEVARQLQQCRLNLGQHLDELEELLYLCFMTINRARNLVLKQIL 334

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT3G49070.11.7e-8249.71Protein of unknown function (DUF677) [more]
AT3G19250.11.6e-2728.45Protein of unknown function (DUF677) [more]
AT1G20180.16.1e-2729.92Protein of unknown function (DUF677) [more]
AT3G19330.13.1e-2328.25Protein of unknown function (DUF677) [more]
AT1G20180.26.9e-2328.70Protein of unknown function (DUF677) [more]
Match NameE-valueIdentityDescription
Q9SMU42.4e-8149.71UPF0496 protein At3g49070 OS=Arabidopsis thaliana OX=3702 GN=At3g49070 PE=2 SV=1[more]
A2XCJ11.4e-4438.42UPF0496 protein 3 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_009784 PE=3 SV=2[more]
Q10RR95.3e-4438.14UPF0496 protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0148000 PE=2 ... [more]
Q9LJK42.2e-2628.45UPF0496 protein At3g19250 OS=Arabidopsis thaliana OX=3702 GN=At3g19250 PE=2 SV=1[more]
Q6DYE58.5e-2629.92UPF0496 protein At1g20180 OS=Arabidopsis thaliana OX=3702 GN=At1g20180 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
XP_038875828.12.9e-19699.72UPF0496 protein At3g49070-like [Benincasa hispida][more]
XP_008436996.14.5e-14979.01PREDICTED: UPF0496 protein At3g49070 isoform X1 [Cucumis melo][more]
XP_031741820.11.4e-14577.62UPF0496 protein At3g49070 [Cucumis sativus] >KAE8647914.1 hypothetical protein C... [more]
XP_022159588.11.8e-13771.94UPF0496 protein At3g49070 [Momordica charantia][more]
XP_023534108.11.1e-9962.15UPF0496 protein At3g49070-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A1S3ATJ22.2e-14979.01UPF0496 protein At3g49070 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482559 PE=... [more]
A0A0A0KN204.2e-14577.78Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G166480 PE=3 SV=1[more]
A0A6J1DZ818.6e-13871.94UPF0496 protein At3g49070 OS=Momordica charantia OX=3673 GN=LOC111025959 PE=3 SV... [more]
A0A6J1H2B81.2e-9962.15UPF0496 protein At3g49070-like OS=Cucurbita moschata OX=3662 GN=LOC111459391 PE=... [more]
A0A6J1JZN77.8e-9962.15UPF0496 protein At3g49070 OS=Cucurbita maxima OX=3661 GN=LOC111491151 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 315..335
NoneNo IPR availablePANTHERPTHR31113:SF6UPF0496 PROTEIN 3coord: 12..359
IPR007749Protein of unknown function DUF677PFAMPF05055DUF677coord: 54..354
e-value: 9.9E-16
score: 57.5
IPR007749Protein of unknown function DUF677PANTHERPTHR31113UPF0496 PROTEIN 3-RELATEDcoord: 12..359

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi12M000442Bhi12M000442mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane