Bhi12G000375 (gene) Wax gourd (B227) v1

Overview


Name	Bhi12G000375
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Vacuolar-processing enzyme
Location	chr12: 13481903 .. 13484183 (+)
RNA-Seq Expression	Bhi12G000375
Synteny	Bhi12G000375

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGCCTCATTGACCTCCAATCTTCTCCTGTTGCTCCTTCTGTTTCTTGCTTACGGCGGCGCCAATGCCTCGCCATGGGACCGGTGGGAGAGAACCATCCGAATGCCGACTGAGAAGGAGGAACTGGGTGGTGGTGGTGACGATGATGATGATCAAAAAGTGGGTACCAGATGGGCAGTTCTTGTAGCTGGTTCTTCTGGGTTTGGGAATTACAGGCATCAGGTATTTGAAATTTGAAAGTAATTTGTGTAAATTAGAATTAGAAATTGATTGATTTTGTGGGGTTTTTTTTTCTTTATTATTATTATTTTTATTTTTATGGGAATAGGCGGATATCTGCCATGCTTATCAACTGTTAAAGAAAGGAGGATTGAAGGATGAGAATATTGTGGTGTTTATGTATGATGATATAGCTTCAAATGTGTTGAATCCAAGGCCTGGAATCATCATCAACCACCCACAAGGAGAGGATGTTTATGCCGGAGTCCCCAAGGTATGTTTAGGAATTTTAAGGCAAATAGCAAAAGTAGCCACAAAAGTTTATAGCCAGATTGAATAAGAGTGTAGAATTCATGGAAATGAACTGATGTTGGTAAAATTTGATGAGACTTCTCTGTTTATATACCAAAAAGCTTAAATGGATAGATAATGGTGAATTTAACGCTTTGTTCATATTCATTATACTGTATAGGATTACACTGGGGAGCATGTGACTGCTCAGAATCTGTATGCTGTGCTTCTGGGAAATAAAACTGCTGTTAATGGAGGGAGTGGGAAAGTTGTGGACAGCAAACCAAATGACAGGATTTTTGTTTACTATTCTGACCATGGAGGCCCTGGAGTACTTGGTTTGTTTTCAAGAACAAGACTAGTTGAATTGATTTCATTTGAATTTTGTTGAACTAAGCTAGTACCTGTCTCTTATACACATCTAGATGTGTATAAGAGACAGATGTTACATGGTGTAAACATTTTTGTAATGTTGCATTTTGTAGGTTATCTATGTAGAAGCATGTGAGAGTGGGAGTATTTTCGAGGGCATAATGCCAAAGGATCTGAATATTTATGTAACCACAGCCTCAAATGCTCAAGAGAGTAGCTTTGGAACTTACTGCCCAGGAATGCAACCGGCACCGCCACCGGAGTACATGACATGTTTAGGAGACTTGTATAGTGTTGCCTGGATGGAAGATAGGTAAAGAAAAGAAAACCTCAATAGCCAATCAAATTTTCAATGAATAACACAAAATCATTCAACTTTTAACATTTACTCGTCTATTCCCAGTGAGACTCACAATCTGAAGAGAGAAACAATAGACCAACAATACCAAACTGTAATGGTTCTTTCTCTGCCACTCAATTACAATTTCTCCTCATTATAATCAACACCCAAAACTTCAGTTTATCAATCTTCAACCATTTGTGGTGCAGGTGAAAAGCAGGACATCAAATCCCAACAATTTGAATGCAGGATCACATGTAATGGAGTACGGAAACTCAAGCATTAAAGCAGAGAGGCTGTACTTGTATCAAGGATTCGACCCTGCTTCAGTGAACTTGCCTCCTTACAATGGCCGGCTCAAAATGAAGAGTATGGAAACAATTAACCAGAGAGATGCAGACATCTTCTTCCTGTGGCAAATGGTAATATAACAATTTCAGCTGCTAATACAGTCCTCAAACAATAAAAAACATGGAGGGTTTTTGGTTCTCCCCCAATTAAACTATTTCTGTTTTGATCTGTTCTCAGTACAAAAAATTTGAAGATGGAACAAATGAGAGAGCCCAAATTCTCCAGGAAATTCGAGAGAGAGTGGCACACAGAGTTCACTTGGATGGAAGCATCAGATTGATTGGTTTTCTTCTGTTTGGACCAGAGAAAGGCCCCAATGTTCTTGATGATGTGAGAGCTCCTGGTTTGCCTCTTGTGGATGATTGGGAATGCCTCAAATCAATGGTACAATTCAGCACCCCCATTTGAATTTTTAGATTTTTAAGAACAAATTGTTAGATGATATAATATTAAATTTGTTTTCACTCATATGAGACGTTATGGTTTATCGAAGGAGACGTTTGTTGATTTGGGAAATTGCAGGTGAGAGTGTTGGAGAGTTATTGTGGTTCACTGACCCAATACGGTATGAAACACATGCGGGCAATTGCAAATATATGCAACAGAGGGGTATCAGAAGCTTCGATGAGGGAAGCTTCAATGGCGGCCTGCAATGGAGGGAGTTATGGGCTGCGGCATCCTTCAAATCAAGGTTACAGTGCTTGA

mRNA sequence

ATGGCTGCCTCATTGACCTCCAATCTTCTCCTGTTGCTCCTTCTGTTTCTTGCTTACGGCGGCGCCAATGCCTCGCCATGGGACCGGTGGGAGAGAACCATCCGAATGCCGACTGAGAAGGAGGAACTGGGTGGTGGTGGTGACGATGATGATGATCAAAAAGTGGGTACCAGATGGGCAGTTCTTGTAGCTGGTTCTTCTGGGTTTGGGAATTACAGGCATCAGGCGGATATCTGCCATGCTTATCAACTGTTAAAGAAAGGAGGATTGAAGGATGAGAATATTGTGGTGTTTATGTATGATGATATAGCTTCAAATGTGTTGAATCCAAGGCCTGGAATCATCATCAACCACCCACAAGGAGAGGATGTTTATGCCGGAGTCCCCAAGGATTACACTGGGGAGCATGTGACTGCTCAGAATCTGTATGCTGTGCTTCTGGGAAATAAAACTGCTGTTAATGGAGGGAGTGGGAAAGTTGTGGACAGCAAACCAAATGACAGGATTTTTGTTTACTATTCTGACCATGGAGGCCCTGGAGTACTTGGTTTGTTTTCAAGAACAAGACTAGTTATCTATGTAGAAGCATGTGAGAGTGGGAGTATTTTCGAGGGCATAATGCCAAAGGATCTGAATATTTATGTAACCACAGCCTCAAATGCTCAAGAGAGTAGCTTTGGAACTTACTGCCCAGGAATGCAACCGGCACCGCCACCGGAGTACATGACATGTTTAGGAGACTTGTATAGTGTTGCCTGGATGGAAGATAGTGAGACTCACAATCTGAAGAGAGAAACAATAGACCAACAATACCAAACTGTGAAAAGCAGGACATCAAATCCCAACAATTTGAATGCAGGATCACATGTAATGGAGTACGGAAACTCAAGCATTAAAGCAGAGAGGCTGTACTTGTATCAAGGATTCGACCCTGCTTCAGTGAACTTGCCTCCTTACAATGGCCGGCTCAAAATGAAGAGTATGGAAACAATTAACCAGAGAGATGCAGACATCTTCTTCCTGTGGCAAATGTACAAAAAATTTGAAGATGGAACAAATGAGAGAGCCCAAATTCTCCAGGAAATTCGAGAGAGAGTGGCACACAGAGTTCACTTGGATGGAAGCATCAGATTGATTGGTTTTCTTCTGTTTGGACCAGAGAAAGGCCCCAATGTTCTTGATGATGTGAGAGCTCCTGGTTTGCCTCTTGTGGATGATTGGGAATGCCTCAAATCAATGGTGAGAGTGTTGGAGAGTTATTGTGGTTCACTGACCCAATACGGTATGAAACACATGCGGGCAATTGCAAATATATGCAACAGAGGGGTATCAGAAGCTTCGATGAGGGAAGCTTCAATGGCGGCCTGCAATGGAGGGAGTTATGGGCTGCGGCATCCTTCAAATCAAGGTTACAGTGCTTGA

Coding sequence (CDS)

ATGGCTGCCTCATTGACCTCCAATCTTCTCCTGTTGCTCCTTCTGTTTCTTGCTTACGGCGGCGCCAATGCCTCGCCATGGGACCGGTGGGAGAGAACCATCCGAATGCCGACTGAGAAGGAGGAACTGGGTGGTGGTGGTGACGATGATGATGATCAAAAAGTGGGTACCAGATGGGCAGTTCTTGTAGCTGGTTCTTCTGGGTTTGGGAATTACAGGCATCAGGCGGATATCTGCCATGCTTATCAACTGTTAAAGAAAGGAGGATTGAAGGATGAGAATATTGTGGTGTTTATGTATGATGATATAGCTTCAAATGTGTTGAATCCAAGGCCTGGAATCATCATCAACCACCCACAAGGAGAGGATGTTTATGCCGGAGTCCCCAAGGATTACACTGGGGAGCATGTGACTGCTCAGAATCTGTATGCTGTGCTTCTGGGAAATAAAACTGCTGTTAATGGAGGGAGTGGGAAAGTTGTGGACAGCAAACCAAATGACAGGATTTTTGTTTACTATTCTGACCATGGAGGCCCTGGAGTACTTGGTTTGTTTTCAAGAACAAGACTAGTTATCTATGTAGAAGCATGTGAGAGTGGGAGTATTTTCGAGGGCATAATGCCAAAGGATCTGAATATTTATGTAACCACAGCCTCAAATGCTCAAGAGAGTAGCTTTGGAACTTACTGCCCAGGAATGCAACCGGCACCGCCACCGGAGTACATGACATGTTTAGGAGACTTGTATAGTGTTGCCTGGATGGAAGATAGTGAGACTCACAATCTGAAGAGAGAAACAATAGACCAACAATACCAAACTGTGAAAAGCAGGACATCAAATCCCAACAATTTGAATGCAGGATCACATGTAATGGAGTACGGAAACTCAAGCATTAAAGCAGAGAGGCTGTACTTGTATCAAGGATTCGACCCTGCTTCAGTGAACTTGCCTCCTTACAATGGCCGGCTCAAAATGAAGAGTATGGAAACAATTAACCAGAGAGATGCAGACATCTTCTTCCTGTGGCAAATGTACAAAAAATTTGAAGATGGAACAAATGAGAGAGCCCAAATTCTCCAGGAAATTCGAGAGAGAGTGGCACACAGAGTTCACTTGGATGGAAGCATCAGATTGATTGGTTTTCTTCTGTTTGGACCAGAGAAAGGCCCCAATGTTCTTGATGATGTGAGAGCTCCTGGTTTGCCTCTTGTGGATGATTGGGAATGCCTCAAATCAATGGTGAGAGTGTTGGAGAGTTATTGTGGTTCACTGACCCAATACGGTATGAAACACATGCGGGCAATTGCAAATATATGCAACAGAGGGGTATCAGAAGCTTCGATGAGGGAAGCTTCAATGGCGGCCTGCAATGGAGGGAGTTATGGGCTGCGGCATCCTTCAAATCAAGGTTACAGTGCTTGA

Protein sequence

MAASLTSNLLLLLLLFLAYGGANASPWDRWERTIRMPTEKEELGGGGDDDDDQKVGTRWAVLVAGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIASNVLNPRPGIIINHPQGEDVYAGVPKDYTGEHVTAQNLYAVLLGNKTAVNGGSGKVVDSKPNDRIFVYYSDHGGPGVLGLFSRTRLVIYVEACESGSIFEGIMPKDLNIYVTTASNAQESSFGTYCPGMQPAPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQYQTVKSRTSNPNNLNAGSHVMEYGNSSIKAERLYLYQGFDPASVNLPPYNGRLKMKSMETINQRDADIFFLWQMYKKFEDGTNERAQILQEIRERVAHRVHLDGSIRLIGFLLFGPEKGPNVLDDVRAPGLPLVDDWECLKSMVRVLESYCGSLTQYGMKHMRAIANICNRGVSEASMREASMAACNGGSYGLRHPSNQGYSA

Homology

[Close]

BLAST of Bhi12G000375 vs. TAIR 10
Match: AT1G62710.1 (beta vacuolar processing enzyme )

HSP 1 Score: 631.7 bits (1628), Expect = 4.7e-181
Identity = 326/496 (65.73%), Postives = 370/496 (74.60%), Query Frame = 0


Query: 1   MAASLTSNLLLLLLLFLAYGGANASPWDRWERTIRMPTEKEELGGGGDDDDDQKVGTRWA 60
           MA S      LLLLL L     +A    R+E  I MPTE+        D D+  VGTRWA
Sbjct: 1   MAKSCYFRPALLLLLVLL---VHAESRGRFEPKILMPTEE----ANPADQDEDGVGTRWA 60

Query: 61  VLVAGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIASNVLNPRPGIIINHPQ 120
           VLVAGSSG+GNYRHQAD+CHAYQ+L+KGGLK+ENIVV MYDDIA++ LNPRPG +INHP 
Sbjct: 61  VLVAGSSGYGNYRHQADVCHAYQILRKGGLKEENIVVLMYDDIANHPLNPRPGTLINHPD 120

Query: 121 GEDVYAGVPKDYTGEHVTAQNLYAVLLGNKTAVNGGSGKVVDSKPNDRIFVYYSDHGGPG 180
           G+DVYAGVPKDYTG  VTA N YAVLLG++ AV GGSGKV+ SKPND IFVYY+DHGGPG
Sbjct: 121 GDDVYAGVPKDYTGSSVTAANFYAVLLGDQKAVKGGSGKVIASKPNDHIFVYYADHGGPG 180

Query: 181 VLGL----------FSRT-----------RLVIYVEACESGSIFEGIMPKDLNIYVTTAS 240
           VLG+          F  T            +VIYVEACESGSIFEGIMPKDLNIYVTTAS
Sbjct: 181 VLGMPNTPHIYAADFIETLKKKHASGTYKEMVIYVEACESGSIFEGIMPKDLNIYVTTAS 240

Query: 241 NAQESSFGTYCPGMQPAPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQYQTVKSRTS 300
           NAQESS+GTYCPGM P+PP EY+TCLGDLYSVAWMEDSETHNLK+ETI QQY TVK RTS
Sbjct: 241 NAQESSYGTYCPGMNPSPPSEYITCLGDLYSVAWMEDSETHNLKKETIKQQYHTVKMRTS 300

Query: 301 NPNNLNAGSHVMEYGNSSIKAERLYLYQGFDPASVNLPPYNGRLKMKSMETINQRDADIF 360
           N N  + GSHVMEYGN+SIK+E+LYLYQGFDPA+VNLP     +K K +  +NQRDAD+ 
Sbjct: 301 NYNTYSGGSHVMEYGNNSIKSEKLYLYQGFDPATVNLPLNELPVKSK-IGVVNQRDADLL 360

Query: 361 FLWQMYKKFEDGTNERAQILQEIRERVAHRVHLDGSIRLIGFLLFGPEKGPNVLDDVRAP 420
           FLW MY+  EDG+ ++   L+E+ E   HR HLD S+ LI  +LFGP    NVL+ VR P
Sbjct: 361 FLWHMYRTSEDGSRKKDDTLKELTETTRHRKHLDASVELIATILFGPTM--NVLNLVREP 420

Query: 421 GLPLVDDWECLKSMVRVLESYCGSLTQYGMKHMRAIANICNRGVSEASMREASMAACNGG 474
           GLPLVDDWECLKSMVRV E +CGSLTQYGMKHMRA AN+CN GVS+  M EAS AAC G 
Sbjct: 421 GLPLVDDWECLKSMVRVFEEHCGSLTQYGMKHMRAFANVCNNGVSKELMEEASTAACGGY 480

[Close]

BLAST of Bhi12G000375 vs. TAIR 10
Match: AT4G32940.1 (gamma vacuolar processing enzyme )

HSP 1 Score: 541.2 bits (1393), Expect = 8.3e-154
Identity = 266/486 (54.73%), Postives = 336/486 (69.14%), Query Frame = 0


Query: 9   LLLLLLLFLAYGGANASPWDRWERTIRMPTEKEELGGGGDDDDDQKVGTRWAVLVAGSSG 68
           L +LL+  +A   A + P D     I++P++        ++DDD   GTRWAVLVAGSSG
Sbjct: 14  LFVLLVSLVAVSAARSGPDD----VIKLPSQASRFFRPAENDDDSNSGTRWAVLVAGSSG 73

Query: 69  FGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIASNVLNPRPGIIINHPQGEDVYAGV 128
           + NYRHQADICHAYQLL+KGGLK+ENIVVFMYDDIA+N  NPRPG IIN P G+DVY GV
Sbjct: 74  YWNYRHQADICHAYQLLRKGGLKEENIVVFMYDDIANNYENPRPGTIINSPHGKDVYQGV 133

Query: 129 PKDYTGEHVTAQNLYAVLLGNKTAVNGGSGKVVDSKPNDRIFVYYSDHGGPGVLG----- 188
           PKDYTG+ V   NL+AV+LG+KTAV GGSGKVVDS PND IF++YSDHGGPGVLG     
Sbjct: 134 PKDYTGDDVNVDNLFAVILGDKTAVKGGSGKVVDSGPNDHIFIFYSDHGGPGVLGMPTSP 193

Query: 189 ----------------LFSRTRLVIYVEACESGSIFEGIMPKDLNIYVTTASNAQESSFG 248
                           L +   LV Y+EACESGSIFEG++P+ LNIY TTASNA+ESS+G
Sbjct: 194 YLYANDLNDVLKKKHALGTYKSLVFYLEACESGSIFEGLLPEGLNIYATTASNAEESSWG 253

Query: 249 TYCPGMQPAPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQYQTVKSRTSNPNNLNAG 308
           TYCPG +P+PPPEY TCLGDLYSVAWMEDS  HNL+ ET+ QQY+ VK RT+ P   + G
Sbjct: 254 TYCPGEEPSPPPEYETCLGDLYSVAWMEDSGMHNLQTETLHQQYELVKRRTA-PVGYSYG 313

Query: 309 SHVMEYGNSSIKAERLYLYQGFDPASVNLPPYNGRLKMKSMETINQRDADIFFLWQMYKK 368
           SHVM+YG+  I  + L LY G +PA+ N    +           NQRDAD+   W+ Y+K
Sbjct: 314 SHVMQYGDVGISKDNLDLYMGTNPANDNFTFADANSLKPPSRVTNQRDADLVHFWEKYRK 373

Query: 369 FEDGTNERAQILQEIRERVAHRVHLDGSIRLIGFLLFGPEKGPNVLDDVRAPGLPLVDDW 428
             +G+  + +  +++ E ++HR+H+D S+ L+G +LFG  +GP VL+ VR+ G PLVDDW
Sbjct: 374 APEGSARKTEAQKQVLEAMSHRLHIDNSVILVGKILFGISRGPEVLNKVRSAGQPLVDDW 433

Query: 429 ECLKSMVRVLESYCGSLTQYGMKHMRAIANICNRGVSEASMREASMAACNGGSYGLRHPS 474
            CLK+ VR  E +CGSL+QYG+KHMR+ ANICN G+    M EA+  AC     G     
Sbjct: 434 NCLKNQVRAFERHCGSLSQYGIKHMRSFANICNAGIQMEQMEEAASQACTTLPTGPWSSL 493

[Close]

BLAST of Bhi12G000375 vs. TAIR 10
Match: AT2G25940.1 (alpha-vacuolar processing enzyme )

HSP 1 Score: 518.5 bits (1334), Expect = 5.8e-147
Identity = 261/474 (55.06%), Postives = 325/474 (68.57%), Query Frame = 0


Query: 4   SLTSNLLLLLLLFLAYGGANASPWDRWERTIRMPTEKEELGGGGDDDDDQKVGTRWAVLV 63
           ++ S L L L L  A  G            I++P+   +     ++DDD    T+WAVLV
Sbjct: 3   TVVSFLALFLFLVAAVSG----------DVIKLPSLASKFFRPTENDDD---STKWAVLV 62

Query: 64  AGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIASNVLNPRPGIIINHPQGED 123
           AGSSG+ NYRHQAD+CHAYQLLKKGG+K+ENIVVFMYDDIA N  NPRPG+IIN P GED
Sbjct: 63  AGSSGYWNYRHQADVCHAYQLLKKGGVKEENIVVFMYDDIAKNEENPRPGVIINSPNGED 122

Query: 124 VYAGVPKDYTGEHVTAQNLYAVLLGNKTAVNGGSGKVVDSKPNDRIFVYYSDHGGPGVLG 183
           VY GVPKDYTG+ V   NL AV+LGNKTA+ GGSGKVVDS PND IF+YYSDHGGPGVLG
Sbjct: 123 VYNGVPKDYTGDEVNVDNLLAVILGNKTALKGGSGKVVDSGPNDHIFIYYSDHGGPGVLG 182

Query: 184 LFSRTR---------------------LVIYVEACESGSIFEGIMPKDLNIYVTTASNAQ 243
           + +                        LV Y+EACESGSIFEG++P+ LNIY TTASNA+
Sbjct: 183 MPTSPNLYANDLNDVLKKKYASGTYKSLVFYLEACESGSIFEGLLPEGLNIYATTASNAE 242

Query: 244 ESSFGTYCPGMQPAPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQYQTVKSRTSNPN 303
           ESS+GTYCPG  P+PP EY TCLGDLYSVAW+EDSE HNL+ ET+ +QY+ VK RT+  +
Sbjct: 243 ESSWGTYCPGEDPSPPSEYETCLGDLYSVAWIEDSEKHNLQTETLHEQYELVKKRTAG-S 302

Query: 304 NLNAGSHVMEYGNSSIKAERLYLYQGFDPASVNLPPYNGRLKMKSMETINQRDADIFFLW 363
             + GSHVME+G+  +  E+L L+ G +PA  N    N           NQRDAD+   W
Sbjct: 303 GKSYGSHVMEFGDIGLSKEKLVLFMGTNPADENFTFVNENSIRPPSRVTNQRDADLVHFW 362

Query: 364 QMYKKFEDGTNERAQILQEIRERVAHRVHLDGSIRLIGFLLFGPEKGPNVLDDVRAPGLP 423
             Y+K  +G+  + +  +++ E ++HR+H+D SI LIG LLFG E G  VL+ VR  G P
Sbjct: 363 HKYQKAPEGSARKVEAQKQVLEAMSHRLHVDNSILLIGILLFGLE-GHAVLNKVRPSGEP 422

Query: 424 LVDDWECLKSMVRVLESYCGSLTQYGMKHMRAIANICNRGVSEASMREASMAAC 457
           LVDDW+CLKS+VR  E +CGSL+QYG+KHMR+IAN+CN G+    M EA+M AC
Sbjct: 423 LVDDWDCLKSLVRAFERHCGSLSQYGIKHMRSIANMCNAGIQMRQMEEAAMQAC 461

[Close]

BLAST of Bhi12G000375 vs. TAIR 10
Match: AT3G20210.1 (delta vacuolar processing enzyme )

HSP 1 Score: 397.9 bits (1021), Expect = 1.1e-110
Identity = 200/432 (46.30%), Postives = 271/432 (62.73%), Query Frame = 0


Query: 48  DDDDDQKVGTRWAVLVAGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIASNV 107
           +  D    GTRWAVLVAGS+ + NYRHQADICHAYQ+L+KGGLKDENI+VFMYDDIA + 
Sbjct: 36  ESSDKSAKGTRWAVLVAGSNEYYNYRHQADICHAYQILRKGGLKDENIIVFMYDDIAFSS 95

Query: 108 LNPRPGIIINHPQGEDVYAGVPKDYTGEHVTAQNLYAVLLGNKTAVNGGSGKVVDSKPND 167
            NPRPG+IIN P GEDVY GVPKDYT E V  QN Y VLLGN++ V GG+GKVV S PND
Sbjct: 96  ENPRPGVIINKPDGEDVYKGVPKDYTKEAVNVQNFYNVLLGNESGVTGGNGKVVKSGPND 155

Query: 168 RIFVYYSDHGGPGVLGL----------FSR-----------TRLVIYVEACESGSIFEGI 227
            IF+YY+DHG PG++ +          F+             ++VIYVEACESGS+FEGI
Sbjct: 156 NIFIYYADHGAPGLIAMPTGDEVMAKDFNEVLEKMHKRKKYNKMVIYVEACESGSMFEGI 215

Query: 228 MPKDLNIYVTTASNAQESSFGTYCPGMQPAPPPEYMTCLGDLYSVAWMEDSETHNLKRET 287
           + K+LNIY  TA+N++ESS+G YCP   P PP E  TCLGD +S++W+EDS+ H++ +ET
Sbjct: 216 LKKNLNIYAVTAANSKESSWGVYCPESYPPPPSEIGTCLGDTFSISWLEDSDLHDMSKET 275

Query: 288 IDQQYQTVKSRTSNPNNLNAGSHVMEYGNSSIKAERLYLYQGFDPASVNLP-PYNGRLKM 347
           ++QQY  VK R    +++   SHV  +G   +  + L  Y G +P + N     +    +
Sbjct: 276 LEQQYHVVKRRVG--SDVPETSHVCRFGTEKMLKDYLSSYIGRNPENDNFTFTESFSSPI 335

Query: 348 KSMETINQRDADIFFLWQMYKKFEDGTNERAQILQEIRERVAHRVHLDGSIRLIGFLLFG 407
            +   +N RD  + +L +  +K   G+ E  +  +++ +   HR  +D SI  I  L   
Sbjct: 336 SNSGLVNPRDIPLLYLQRKIQKAPMGSLESKEAQKKLLDEKNHRKQIDQSITDILRLSVK 395

Query: 408 PEKGPNVLDDVRAPGLPLVDDWECLKSMVRVLESYCGSLTQYGMKHMRAIANICNRGVSE 458
                N+L   R  G PLVDDW+C K++V   +++CG+   YG+K+  A+ANICN GV  
Sbjct: 396 QTNVLNLLTSTRTTGQPLVDDWDCFKTLVNSFKNHCGATVHYGLKYTGALANICNMGVDV 455

[Close]

BLAST of Bhi12G000375 vs. TAIR 10
Match: AT3G20210.2 (delta vacuolar processing enzyme )

HSP 1 Score: 396.0 bits (1016), Expect = 4.3e-110
Identity = 200/432 (46.30%), Postives = 270/432 (62.50%), Query Frame = 0


Query: 48  DDDDDQKVGTRWAVLVAGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIASNV 107
           +  D    GTRWAVLVAGS+ + NYRHQADICHAYQ+L+KGGLKDENI+VFMYDDIA + 
Sbjct: 36  ESSDKSAKGTRWAVLVAGSNEYYNYRHQADICHAYQILRKGGLKDENIIVFMYDDIAFSS 95

Query: 108 LNPRPGIIINHPQGEDVYAGVPKDYTGEHVTAQNLYAVLLGNKTAVNGGSGKVVDSKPND 167
            NPRPG+IIN P GEDVY GVPKDYT E V  QN Y VLLGN++ V GG+GKVV S PND
Sbjct: 96  ENPRPGVIINKPDGEDVYKGVPKDYTKEAVNVQNFYNVLLGNESGVTGGNGKVVKSGPND 155

Query: 168 RIFVYYSDHGGPGVLGL----------FSR-----------TRLVIYVEACESGSIFEGI 227
            IF+YY+DHG PG++ +          F+             ++VIYVEACESGS+FEGI
Sbjct: 156 NIFIYYADHGAPGLIAMPTGDEVMAKDFNEVLEKMHKRKKYNKMVIYVEACESGSMFEGI 215

Query: 228 MPKDLNIYVTTASNAQESSFGTYCPGMQPAPPPEYMTCLGDLYSVAWMEDSETHNLKRET 287
           + K+LNIY  TA+N++ESS+G YCP   P PP E  TCLGD +S++W+EDS+ H++ +ET
Sbjct: 216 LKKNLNIYAVTAANSKESSWGVYCPESYPPPPSEIGTCLGDTFSISWLEDSDLHDMSKET 275

Query: 288 IDQQYQTVKSRTSNPNNLNAGSHVMEYGNSSIKAERLYLYQGFDPASVNLP-PYNGRLKM 347
           ++QQY  VK R    +++   SHV  +G   +  + L  Y G +P + N     +    +
Sbjct: 276 LEQQYHVVKRRVG--SDVPETSHVCRFGTEKMLKDYLSSYIGRNPENDNFTFTESFSSPI 335

Query: 348 KSMETINQRDADIFFLWQMYKKFEDGTNERAQILQEIRERVAHRVHLDGSIRLIGFLLFG 407
            +   +N RD  + +L +  +K   G+ E  +  +++ +   HR  +D SI  I  L   
Sbjct: 336 SNSGLVNPRDIPLLYLQRKIQKAPMGSLESKEAQKKLLDEKNHRKQIDQSITDILRLSVK 395

Query: 408 PEKGPNVLDDVRAPGLPLVDDWECLKSMVRVLESYCGSLTQYGMKHMRAIANICNRGVSE 458
                N+L   R  G PLVDDW+C K++V   +++CG+   YG+K+  A+ANICN GV  
Sbjct: 396 QTNVLNLLTSTRTTGQPLVDDWDCFKTLVNSFKNHCGATVHYGLKYTGALANICNMGVDV 455

[Close]

BLAST of Bhi12G000375 vs. ExPASy Swiss-Prot
Match: P49042 (Vacuolar-processing enzyme OS=Ricinus communis OX=3988 PE=1 SV=1)

HSP 1 Score: 674.9 bits (1740), Expect = 6.8e-193
Identity = 329/485 (67.84%), Postives = 384/485 (79.18%), Query Frame = 0


Query: 10  LLLLLLFLAYGGANASPWDRWERTIRMPTEKEELGGGGDDDDDQKVGTRWAVLVAGSSGF 69
           L+  L FL   G  AS  + +E  I MPTE+ E       DDD ++GTRWAVLVAGS GF
Sbjct: 17  LVFTLSFLPIPGLLASRLNPFEPGILMPTEEAE---PVQVDDDDQLGTRWAVLVAGSMGF 76

Query: 70  GNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIASNVLNPRPGIIINHPQGEDVYAGVP 129
           GNYRHQAD+CHAYQLL+KGGLK+ENI+VFMYDDIA N LNPRPG+IINHPQGEDVYAGVP
Sbjct: 77  GNYRHQADVCHAYQLLRKGGLKEENIIVFMYDDIAKNELNPRPGVIINHPQGEDVYAGVP 136

Query: 130 KDYTGEHVTAQNLYAVLLGNKTAVNGGSGKVVDSKPNDRIFVYYSDHGGPGVLGL----- 189
           KDYTGEHVTA+NLYAVLLG+K+AV GGSGKVVDSKPNDRIF+YYSDHGGPGVLG+     
Sbjct: 137 KDYTGEHVTAKNLYAVLLGDKSAVQGGSGKVVDSKPNDRIFLYYSDHGGPGVLGMPNLPY 196

Query: 190 ----------------FSRTRLVIYVEACESGSIFEGIMPKDLNIYVTTASNAQESSFGT 249
                               ++VIYVEACESGSIFEGIMPKD++IYVTTASNAQESS+GT
Sbjct: 197 LYAMDFIEVLKKKHAAGGYKKMVIYVEACESGSIFEGIMPKDVDIYVTTASNAQESSWGT 256

Query: 250 YCPGMQPAPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQYQTVKSRTSNPNNLNAGS 309
           YCPGM+P+PPPE+ TCLGDLYSVAWMEDSE+HNLK+ET+ QQY +VK+RTSN N   AGS
Sbjct: 257 YCPGMEPSPPPEFTTCLGDLYSVAWMEDSESHNLKKETVKQQYSSVKARTSNYNTYAAGS 316

Query: 310 HVMEYGNSSIKAERLYLYQGFDPASVNLPPYNGRLKMKSMETINQRDADIFFLWQMYKKF 369
           HVM+YGN SIKA++LYL+QGFDPASVN PP N  L    ME +NQRDA++ F+WQ+YK+ 
Sbjct: 317 HVMQYGNQSIKADKLYLFQGFDPASVNFPPNNAHLN-APMEVVNQRDAELHFMWQLYKRS 376

Query: 370 EDGTNERAQILQEIRERVAHRVHLDGSIRLIGFLLFGPEKGPNVLDDVRAPGLPLVDDWE 429
           E+G+ ++ +ILQ+I++ + HR HLD S++LIG LLFGP+K   +L  VR PG PLVDDW 
Sbjct: 377 ENGSEKKKEILQQIKDAIKHRSHLDSSMQLIGDLLFGPKKASAILKSVREPGSPLVDDWG 436

Query: 430 CLKSMVRVLESYCGSLTQYGMKHMRAIANICNRGVSEASMREASMAACNGGSYGLRHPSN 474
           CLKSMVRV E+ CGSLTQYGMKHMR  ANICN GVS  SM EA  AAC+G   G  HP+N
Sbjct: 437 CLKSMVRVFETCCGSLTQYGMKHMRTFANICNAGVSHTSMEEACNAACSGHDAGQWHPTN 496

[Close]

BLAST of Bhi12G000375 vs. ExPASy Swiss-Prot
Match: P49045 (Vacuolar-processing enzyme OS=Glycine max OX=3847 PE=2 SV=1)

HSP 1 Score: 664.1 bits (1712), Expect = 1.2e-189
Identity = 317/486 (65.23%), Postives = 383/486 (78.81%), Query Frame = 0


Query: 9   LLLLLLLFLAYGGANASPWDRWERTIRMPTEKEELGGGGDDDDDQKVGTRWAVLVAGSSG 68
           L ++++L   +G A       W+  I++PTE         D D  +VGTRWAVLVAGS+G
Sbjct: 18  LWMMVVLVRVHGAAARPNRKEWDSVIKLPTEPV-------DADSDEVGTRWAVLVAGSNG 77

Query: 69  FGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIASNVLNPRPGIIINHPQGEDVYAGV 128
           +GNYRHQAD+CHAYQLL KGGLK+ENIVVFMYDDIA+N LNPR G+IINHP+GED+YAGV
Sbjct: 78  YGNYRHQADVCHAYQLLIKGGLKEENIVVFMYDDIATNELNPRHGVIINHPEGEDLYAGV 137

Query: 129 PKDYTGEHVTAQNLYAVLLGNKTAVNGGSGKVVDSKPNDRIFVYYSDHGGPGVLGL---- 188
           PKDYTG++VT +NL+AV+LG+K+ + GGSGKV++SKP DRIF+YYSDHGGPG+LG+    
Sbjct: 138 PKDYTGDNVTTENLFAVILGDKSKLKGGSGKVINSKPEDRIFIYYSDHGGPGILGMPNMP 197

Query: 189 -----------------FSRTRLVIYVEACESGSIFEGIMPKDLNIYVTTASNAQESSFG 248
                             S   +VIYVEACESGS+FEGIMPKDLNIYVTTASNAQE+S+G
Sbjct: 198 YLYAMDFIDVLKKKHASGSYKEMVIYVEACESGSVFEGIMPKDLNIYVTTASNAQENSWG 257

Query: 249 TYCPGMQPAPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQYQTVKSRTSNPNNLNAG 308
           TYCPGM P+PPPEY+TCLGDLYSVAWMEDSE HNLKRE++ QQY++VK RTSN NN   G
Sbjct: 258 TYCPGMDPSPPPEYITCLGDLYSVAWMEDSEAHNLKRESVKQQYKSVKQRTSNFNNYAMG 317

Query: 309 SHVMEYGNSSIKAERLYLYQGFDPASVNLPPYNGRLKMKSMETINQRDADIFFLWQMYKK 368
           SHVM+YG+++I AE+LYLYQGFDPA+VN PP NGRL+ K ME +NQRDA++F LWQMY++
Sbjct: 318 SHVMQYGDTNITAEKLYLYQGFDPATVNFPPQNGRLETK-MEVVNQRDAELFLLWQMYQR 377

Query: 369 FEDGTNERAQILQEIRERVAHRVHLDGSIRLIGFLLFGPEKGPNVLDDVRAPGLPLVDDW 428
               +  +  IL++I E V HR H+DGS+ LIG LL+GP KG +VL  VRAPG  LVDDW
Sbjct: 378 SNHQSENKTDILKQIAETVKHRKHIDGSVELIGVLLYGPGKGSSVLQSVRAPGSSLVDDW 437

Query: 429 ECLKSMVRVLESYCGSLTQYGMKHMRAIANICNRGVSEASMREASMAACNGGSYGLRHPS 474
            CLKSMVRV E++CG+LTQYGMKHMRA ANICN GVSEASM EA +AAC G + GL HPS
Sbjct: 438 TCLKSMVRVFETHCGTLTQYGMKHMRAFANICNSGVSEASMEEACLAACEGYNAGLFHPS 495

[Close]

BLAST of Bhi12G000375 vs. ExPASy Swiss-Prot
Match: P49046 (Legumain OS=Canavalia ensiformis OX=3823 PE=1 SV=1)

HSP 1 Score: 654.1 bits (1686), Expect = 1.2e-186
Identity = 312/486 (64.20%), Postives = 375/486 (77.16%), Query Frame = 0


Query: 9   LLLLLLLFLAYGGANASPWDRWERTIRMPTEKEELGGGGDDDDDQKVGTRWAVLVAGSSG 68
           ++++L++   +G A       W+  I++PTE            D +VGTRWAVLVAGS+G
Sbjct: 1   MVMMLVMLSLHGTAARLNRREWDSVIQLPTEPV----------DDEVGTRWAVLVAGSNG 60

Query: 69  FGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIASNVLNPRPGIIINHPQGEDVYAGV 128
           +GNYRHQAD+CHAYQLL KGG+K+ENIVVFMYDDIA N +NPRPG+IINHPQG DVYAGV
Sbjct: 61  YGNYRHQADVCHAYQLLIKGGVKEENIVVFMYDDIAYNAMNPRPGVIINHPQGPDVYAGV 120

Query: 129 PKDYTGEHVTAQNLYAVLLGNKTAVNGGSGKVVDSKPNDRIFVYYSDHGGPGVLGLFSR- 188
           PKDYTGE VT +NLYAV+LG+K+ V GGSGKV++S P DRIF++YSDHGGPGVLG+ +  
Sbjct: 121 PKDYTGEDVTPENLYAVILGDKSKVKGGSGKVINSNPEDRIFIFYSDHGGPGVLGMPNAP 180

Query: 189 --------------------TRLVIYVEACESGSIFEGIMPKDLNIYVTTASNAQESSFG 248
                                 +VIY+EACESGSIFEGIMPKDLNIYVTTASNAQE+SFG
Sbjct: 181 FVYAMDFIDVLKKKHASGGYKEMVIYIEACESGSIFEGIMPKDLNIYVTTASNAQENSFG 240

Query: 249 TYCPGMQPAPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQYQTVKSRTSNPNNLNAG 308
           TYCPGM P PP EY+TCLGDLYSV+WMEDSETHNLKRET+ QQYQ+V+ RTSN N+   G
Sbjct: 241 TYCPGMNPPPPEEYVTCLGDLYSVSWMEDSETHNLKRETVQQQYQSVRKRTSNSNSYRFG 300

Query: 309 SHVMEYGNSSIKAERLYLYQGFDPASVNLPPYNGRLKMKSMETINQRDADIFFLWQMYKK 368
           SHVM+YG+++I AE+LYLY GFDPA+VN PP+NG L+ K ME +NQRDA++ F+WQMY++
Sbjct: 301 SHVMQYGDTNITAEKLYLYHGFDPATVNFPPHNGNLEAK-MEVVNQRDAELLFMWQMYQR 360

Query: 369 FEDGTNERAQILQEIRERVAHRVHLDGSIRLIGFLLFGPEKGPNVLDDVRAPGLPLVDDW 428
                 ++  IL++I E V HR HLDGS+ LIG LL+GP K  +VL  VRAPGLPLVDDW
Sbjct: 361 SNHQPEKKTHILEQITETVKHRNHLDGSVELIGVLLYGPGKSSSVLHSVRAPGLPLVDDW 420

Query: 429 ECLKSMVRVLESYCGSLTQYGMKHMRAIANICNRGVSEASMREASMAACNGGSYGLRHPS 474
            CLKSMVRV E++CGSLTQYGMKHMRA  N+CN GVS+ASM EA  AAC G   GL +PS
Sbjct: 421 TCLKSMVRVFETHCGSLTQYGMKHMRAFGNVCNSGVSKASMEEACKAACGGYDAGLLYPS 475

[Close]

BLAST of Bhi12G000375 vs. ExPASy Swiss-Prot
Match: O24326 (Vacuolar-processing enzyme OS=Phaseolus vulgaris OX=3885 PE=2 SV=1)

HSP 1 Score: 642.9 bits (1657), Expect = 2.9e-183
Identity = 312/483 (64.60%), Postives = 375/483 (77.64%), Query Frame = 0


Query: 12  LLLLFLAYGGANASPWDRWERTIRMPTEKEELGGGGDDDDDQKVGTRWAVLVAGSSGFGN 71
           +L++ +   G N    D     I++PT++        D +  +VGTRWAVLVAGS+G+GN
Sbjct: 22  MLVMVMRIQGTNGKEQD---SVIKLPTQEV-------DAESDEVGTRWAVLVAGSNGYGN 81

Query: 72  YRHQADICHAYQLLKKGGLKDENIVVFMYDDIASNVLNPRPGIIINHPQGEDVYAGVPKD 131
           YRHQAD+CHAYQLL KGG+K+ENIVVFMYDDIA++ LNPRPG+IIN+PQG DVYAGVPKD
Sbjct: 82  YRHQADVCHAYQLLIKGGVKEENIVVFMYDDIATHELNPRPGVIINNPQGPDVYAGVPKD 141

Query: 132 YTGEHVTAQNLYAVLLGNKTAVNGGSGKVVDSKPNDRIFVYYSDHGGPGVLGLFSR---- 191
           YTGE VT+ N +AVLLG+K+ V GGSGKV++SKP DRIFVYYSDHGGPGVLG+ +     
Sbjct: 142 YTGESVTSHNFFAVLLGDKSKVKGGSGKVINSKPEDRIFVYYSDHGGPGVLGMPNMPYLY 201

Query: 192 -----------------TRLVIYVEACESGSIFEGIMPKDLNIYVTTASNAQESSFGTYC 251
                              +VIYVEACESGSIFEGIMPKDLNIYVTTASNAQE+S+GTYC
Sbjct: 202 AMDFIDVLKKKHASGGYKEMVIYVEACESGSIFEGIMPKDLNIYVTTASNAQENSWGTYC 261

Query: 252 PGMQPAPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQYQTVKSRTSNPNNLNAGSHV 311
           PGM P PPPEY+TCLGDLYSVAWMEDSE+HNLK+E+++QQYQ+VK RTSN      GSHV
Sbjct: 262 PGMYPPPPPEYITCLGDLYSVAWMEDSESHNLKKESVEQQYQSVKQRTSNFEAYAMGSHV 321

Query: 312 MEYGNSSIKAERLYLYQGFDPASVNLPPYNGRLKMKSMETINQRDADIFFLWQMYKKFED 371
           M+YG++++ AE+LYLY GFDPA+VN PP+NGRLK K ME +NQRDA++ F+WQ+Y++   
Sbjct: 322 MQYGDANMTAEKLYLYHGFDPATVNFPPHNGRLKSK-MEVVNQRDAELLFMWQVYQRSNH 381

Query: 372 GTNERAQILQEIRERVAHRVHLDGSIRLIGFLLFGPEKGPNVLDDVRAPGLPLVDDWECL 431
              ++  IL++I E V HR HLDGS+ LIG LL+GPEK  +VL  VR  GLPLVDDW CL
Sbjct: 382 LPEKKTDILKQIEEIVKHRKHLDGSVELIGVLLYGPEKASSVLRSVRTTGLPLVDDWTCL 441

Query: 432 KSMVRVLESYCGSLTQYGMKHMRAIANICNRGVSEASMREASMAACNGGSYGLRHPSNQG 474
           KSMVRV E++CGSLTQYGMKHMRA ANICN GVSE SM +A +AAC G   GL HPSN G
Sbjct: 442 KSMVRVYETHCGSLTQYGMKHMRAFANICNSGVSETSMEKACVAACGGYHAGLLHPSNTG 493

[Close]

BLAST of Bhi12G000375 vs. ExPASy Swiss-Prot
Match: Q39044 (Vacuolar-processing enzyme beta-isozyme OS=Arabidopsis thaliana OX=3702 GN=bVPE PE=1 SV=3)

HSP 1 Score: 631.7 bits (1628), Expect = 6.6e-180
Identity = 326/496 (65.73%), Postives = 370/496 (74.60%), Query Frame = 0


Query: 1   MAASLTSNLLLLLLLFLAYGGANASPWDRWERTIRMPTEKEELGGGGDDDDDQKVGTRWA 60
           MA S      LLLLL L     +A    R+E  I MPTE+        D D+  VGTRWA
Sbjct: 1   MAKSCYFRPALLLLLVLL---VHAESRGRFEPKILMPTEE----ANPADQDEDGVGTRWA 60

Query: 61  VLVAGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIASNVLNPRPGIIINHPQ 120
           VLVAGSSG+GNYRHQAD+CHAYQ+L+KGGLK+ENIVV MYDDIA++ LNPRPG +INHP 
Sbjct: 61  VLVAGSSGYGNYRHQADVCHAYQILRKGGLKEENIVVLMYDDIANHPLNPRPGTLINHPD 120

Query: 121 GEDVYAGVPKDYTGEHVTAQNLYAVLLGNKTAVNGGSGKVVDSKPNDRIFVYYSDHGGPG 180
           G+DVYAGVPKDYTG  VTA N YAVLLG++ AV GGSGKV+ SKPND IFVYY+DHGGPG
Sbjct: 121 GDDVYAGVPKDYTGSSVTAANFYAVLLGDQKAVKGGSGKVIASKPNDHIFVYYADHGGPG 180

Query: 181 VLGL----------FSRT-----------RLVIYVEACESGSIFEGIMPKDLNIYVTTAS 240
           VLG+          F  T            +VIYVEACESGSIFEGIMPKDLNIYVTTAS
Sbjct: 181 VLGMPNTPHIYAADFIETLKKKHASGTYKEMVIYVEACESGSIFEGIMPKDLNIYVTTAS 240

Query: 241 NAQESSFGTYCPGMQPAPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQYQTVKSRTS 300
           NAQESS+GTYCPGM P+PP EY+TCLGDLYSVAWMEDSETHNLK+ETI QQY TVK RTS
Sbjct: 241 NAQESSYGTYCPGMNPSPPSEYITCLGDLYSVAWMEDSETHNLKKETIKQQYHTVKMRTS 300

Query: 301 NPNNLNAGSHVMEYGNSSIKAERLYLYQGFDPASVNLPPYNGRLKMKSMETINQRDADIF 360
           N N  + GSHVMEYGN+SIK+E+LYLYQGFDPA+VNLP     +K K +  +NQRDAD+ 
Sbjct: 301 NYNTYSGGSHVMEYGNNSIKSEKLYLYQGFDPATVNLPLNELPVKSK-IGVVNQRDADLL 360

Query: 361 FLWQMYKKFEDGTNERAQILQEIRERVAHRVHLDGSIRLIGFLLFGPEKGPNVLDDVRAP 420
           FLW MY+  EDG+ ++   L+E+ E   HR HLD S+ LI  +LFGP    NVL+ VR P
Sbjct: 361 FLWHMYRTSEDGSRKKDDTLKELTETTRHRKHLDASVELIATILFGPTM--NVLNLVREP 420

Query: 421 GLPLVDDWECLKSMVRVLESYCGSLTQYGMKHMRAIANICNRGVSEASMREASMAACNGG 474
           GLPLVDDWECLKSMVRV E +CGSLTQYGMKHMRA AN+CN GVS+  M EAS AAC G 
Sbjct: 421 GLPLVDDWECLKSMVRVFEEHCGSLTQYGMKHMRAFANVCNNGVSKELMEEASTAACGGY 480

[Close]

BLAST of Bhi12G000375 vs. NCBI nr
Match: XP_038907005.1 (LOW QUALITY PROTEIN: vacuolar-processing enzyme-like [Benincasa hispida])

HSP 1 Score: 963.0 bits (2488), Expect = 9.5e-277
Identity = 473/495 (95.56%), Postives = 473/495 (95.56%), Query Frame = 0


Query: 1   MAASLTSNLLLLLLLFLAYGGANASPWDRWERTIRMPTEKEELGGGGDDDDDQKVGTRWA 60
           MAASLTSNLLLLLLLFLAYGGANASPWDRWERTIRMPTEKEELGGGGDDDDDQKVGTRWA
Sbjct: 1   MAASLTSNLLLLLLLFLAYGGANASPWDRWERTIRMPTEKEELGGGGDDDDDQKVGTRWA 60

Query: 61  VLVAGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIASNVLNPRPGIIINHPQ 120
           VLVAGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIASNVLNPRPGIIINHPQ
Sbjct: 61  VLVAGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIASNVLNPRPGIIINHPQ 120

Query: 121 GEDVYAGVPKDYTGEHVTAQNLYAVLLGNKTAVNGGSGKVVDSKPNDRIFVYYSDHGGPG 180
           GEDVYAGVPKDYTGEHVTAQNLYAVLLGNKTAVNGGSGKVVDSKPNDRIFVYYSDHGGPG
Sbjct: 121 GEDVYAGVPKDYTGEHVTAQNLYAVLLGNKTAVNGGSGKVVDSKPNDRIFVYYSDHGGPG 180

Query: 181 VLGLFSRTRL----------------------VIYVEACESGSIFEGIMPKDLNIYVTTA 240
           VLGLFSRTRL                      VIYVEACESGSIFEGIMPKDLNIYVTTA
Sbjct: 181 VLGLFSRTRLVELISFEFCXTKLVPVSYTHLDVIYVEACESGSIFEGIMPKDLNIYVTTA 240

Query: 241 SNAQESSFGTYCPGMQPAPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQYQTVKSRT 300
           SNAQESSFGTYCPGMQPAPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQYQTVKSRT
Sbjct: 241 SNAQESSFGTYCPGMQPAPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQYQTVKSRT 300

Query: 301 SNPNNLNAGSHVMEYGNSSIKAERLYLYQGFDPASVNLPPYNGRLKMKSMETINQRDADI 360
           SNPNNLNAGSHVMEYGNSSIKAERLYLYQGFDPASVNLPPYNGRLKMKSMETINQRDADI
Sbjct: 301 SNPNNLNAGSHVMEYGNSSIKAERLYLYQGFDPASVNLPPYNGRLKMKSMETINQRDADI 360

Query: 361 FFLWQMYKKFEDGTNERAQILQEIRERVAHRVHLDGSIRLIGFLLFGPEKGPNVLDDVRA 420
           FFLWQMYKKFEDGTNERAQILQEIRERVAHRVHLDGSIRLIGFLLFGPEKGPNVLDDVRA
Sbjct: 361 FFLWQMYKKFEDGTNERAQILQEIRERVAHRVHLDGSIRLIGFLLFGPEKGPNVLDDVRA 420

Query: 421 PGLPLVDDWECLKSMVRVLESYCGSLTQYGMKHMRAIANICNRGVSEASMREASMAACNG 474
           PGLPLVDDWECLKSMVRVLESYCGSLTQYGMKHMRAIANICNRGVSEASMREASMAACNG
Sbjct: 421 PGLPLVDDWECLKSMVRVLESYCGSLTQYGMKHMRAIANICNRGVSEASMREASMAACNG 480

[Close]

BLAST of Bhi12G000375 vs. NCBI nr
Match: KAA0039681.1 (legumain [Cucumis melo var. makuwa])

HSP 1 Score: 894.4 bits (2310), Expect = 4.2e-256
Identity = 437/495 (88.28%), Postives = 453/495 (91.52%), Query Frame = 0


Query: 1   MAASLTSNLLLLLLLFLAYGGANASPWDRWERTIRMPTEKEELGGGGDDDDDQKVGTRWA 60
           MAA  TSNL+LLLLL LAYGGA+ASPWDRWERTIRMPTEKEE+GG G DDDD+KVGTRWA
Sbjct: 1   MAAPSTSNLVLLLLLLLAYGGAHASPWDRWERTIRMPTEKEEVGGSGGDDDDRKVGTRWA 60

Query: 61  VLVAGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIASNVLNPRPGIIINHPQ 120
           VLVAGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIA+NVLNPRPGIIINHPQ
Sbjct: 61  VLVAGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIATNVLNPRPGIIINHPQ 120

Query: 121 GEDVYAGVPKDYTGEHVTAQNLYAVLLGNKTAVNGGSGKVVDSKPNDRIFVYYSDHGGPG 180
           GEDVYAGVPKDYTGEHVTAQNLYAVLLGN+TAVNGGSGKVVDSKPNDRIFVYYSDHGGPG
Sbjct: 121 GEDVYAGVPKDYTGEHVTAQNLYAVLLGNRTAVNGGSGKVVDSKPNDRIFVYYSDHGGPG 180

Query: 181 VLGL---------------------FSRTRLVIYVEACESGSIFEGIMPKDLNIYVTTAS 240
           VLG+                          +VIYVEACESGSIFEGIMP+DLNIYVTTAS
Sbjct: 181 VLGMPNLPFVYAMDFIEVLKKKHAAKGYKEMVIYVEACESGSIFEGIMPRDLNIYVTTAS 240

Query: 241 NAQESSFGTYCPGMQPAPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQYQTVKSRTS 300
           NAQESSFGTYCPGMQPAPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQYQTVK RTS
Sbjct: 241 NAQESSFGTYCPGMQPAPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQYQTVKERTS 300

Query: 301 NPNNLNAGSHVMEYGNSSIKAERLYLYQGFDPASVNLPPYNGRLKMKSMETINQRDADIF 360
           NPNNLN GSHVMEYGNSSIKAERLYLYQGFDPASVNLPPYNGRL+MKSME INQRDADIF
Sbjct: 301 NPNNLNTGSHVMEYGNSSIKAERLYLYQGFDPASVNLPPYNGRLEMKSMEAINQRDADIF 360

Query: 361 FLWQMYKKFEDGTNERAQILQEIRERVAHRVHLDGSIRLIGFLLFGPEKGPNVLDDVRAP 420
           FLWQMY+KFEDGTNE+AQIL+EIRE V HR HLDGSIR+IGFLLFGPEKG NVLDDVRA 
Sbjct: 361 FLWQMYRKFEDGTNEKAQILEEIRETVTHRSHLDGSIRMIGFLLFGPEKGSNVLDDVRAS 420

Query: 421 GLPLVDDWECLKSMVRVLESYCGSLTQYGMKHMRAIANICNRGVSEASMREASMAACN-G 474
           GLPLVDDWECLKSMVRVLESYCGSLTQYGMKHMRA+ANICNRGVSEASMREASMAACN G
Sbjct: 421 GLPLVDDWECLKSMVRVLESYCGSLTQYGMKHMRALANICNRGVSEASMREASMAACNGG 480

[Close]

BLAST of Bhi12G000375 vs. NCBI nr
Match: XP_008437131.1 (PREDICTED: legumain [Cucumis melo])

HSP 1 Score: 894.0 bits (2309), Expect = 5.4e-256
Identity = 437/495 (88.28%), Postives = 453/495 (91.52%), Query Frame = 0


Query: 1   MAASLTSNLLLLLLLFLAYGGANASPWDRWERTIRMPTEKEELGGGGDDDDDQKVGTRWA 60
           MAA  TSNL+LLLLL LAYGGA+ASPWDRWERTIRMPTE+EE+GG G DDDD+KVGTRWA
Sbjct: 1   MAAPSTSNLVLLLLLLLAYGGAHASPWDRWERTIRMPTEEEEVGGSGGDDDDRKVGTRWA 60

Query: 61  VLVAGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIASNVLNPRPGIIINHPQ 120
           VLVAGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIA+NVLNPRPGIIINHPQ
Sbjct: 61  VLVAGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIATNVLNPRPGIIINHPQ 120

Query: 121 GEDVYAGVPKDYTGEHVTAQNLYAVLLGNKTAVNGGSGKVVDSKPNDRIFVYYSDHGGPG 180
           GEDVYAGVPKDYTGEHVTAQNLYAVLLGN+TAVNGGSGKVVDSKPNDRIFVYYSDHGGPG
Sbjct: 121 GEDVYAGVPKDYTGEHVTAQNLYAVLLGNRTAVNGGSGKVVDSKPNDRIFVYYSDHGGPG 180

Query: 181 VLGL---------------------FSRTRLVIYVEACESGSIFEGIMPKDLNIYVTTAS 240
           VLG+                          +VIYVEACESGSIFEGIMPKDLNIYVTTAS
Sbjct: 181 VLGMPNLPFVYAMDFIEVLKKKHAAKGYKEMVIYVEACESGSIFEGIMPKDLNIYVTTAS 240

Query: 241 NAQESSFGTYCPGMQPAPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQYQTVKSRTS 300
           NAQESSFGTYCPGMQPAPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQYQTVK RTS
Sbjct: 241 NAQESSFGTYCPGMQPAPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQYQTVKERTS 300

Query: 301 NPNNLNAGSHVMEYGNSSIKAERLYLYQGFDPASVNLPPYNGRLKMKSMETINQRDADIF 360
           NPNNLN GSHVMEYGNSSIKAERLYLYQGFDPASVNLPPYNGRL+MKSME INQRDADIF
Sbjct: 301 NPNNLNTGSHVMEYGNSSIKAERLYLYQGFDPASVNLPPYNGRLEMKSMEAINQRDADIF 360

Query: 361 FLWQMYKKFEDGTNERAQILQEIRERVAHRVHLDGSIRLIGFLLFGPEKGPNVLDDVRAP 420
           FLWQMY+KFEDGTNE+AQIL+EIRE V HR HLDGSIR+IGFLLFGPEKG NVLDDVRA 
Sbjct: 361 FLWQMYRKFEDGTNEKAQILEEIRETVTHRSHLDGSIRMIGFLLFGPEKGSNVLDDVRAS 420

Query: 421 GLPLVDDWECLKSMVRVLESYCGSLTQYGMKHMRAIANICNRGVSEASMREASMAACN-G 474
           GLPLVDDWECLKSMVRVLESYCGSLTQYGMKHMRA+ANICNRGVSEASMREASMAACN G
Sbjct: 421 GLPLVDDWECLKSMVRVLESYCGSLTQYGMKHMRALANICNRGVSEASMREASMAACNGG 480

[Close]

BLAST of Bhi12G000375 vs. NCBI nr
Match: XP_004147613.1 (legumain [Cucumis sativus] >KGN50214.1 hypothetical protein Csa_000360 [Cucumis sativus])

HSP 1 Score: 876.3 bits (2263), Expect = 1.2e-250
Identity = 425/494 (86.03%), Postives = 448/494 (90.69%), Query Frame = 0


Query: 1   MAASLTSNLLLLLLLFLAYGGANASPWDRWERTIRMPTEKEELGGGGDDDDDQKVGTRWA 60
           MA+  TSNL+L LLLFLAYG A+ASPWDRWERTIRMPTEKEE+GG G    D+KVGTRWA
Sbjct: 1   MASPSTSNLVLFLLLFLAYGCADASPWDRWERTIRMPTEKEEMGGAG----DRKVGTRWA 60

Query: 61  VLVAGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIASNVLNPRPGIIINHPQ 120
           VL+AGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIA+NVLNPRPGIIINHPQ
Sbjct: 61  VLIAGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIATNVLNPRPGIIINHPQ 120

Query: 121 GEDVYAGVPKDYTGEHVTAQNLYAVLLGNKTAVNGGSGKVVDSKPNDRIFVYYSDHGGPG 180
           GEDVYAGVPKDYTGEHVTAQNLYAVLLGN+TAV+GGSGKVVDSKPNDRIFVYYSDHGGPG
Sbjct: 121 GEDVYAGVPKDYTGEHVTAQNLYAVLLGNRTAVDGGSGKVVDSKPNDRIFVYYSDHGGPG 180

Query: 181 VLGL---------------------FSRTRLVIYVEACESGSIFEGIMPKDLNIYVTTAS 240
           VLG+                          +VIYVEACESGSIFEGI+PKDLNIYVTTAS
Sbjct: 181 VLGMPNLPFVYAMDFIEVLKKKHAAKGYKEMVIYVEACESGSIFEGILPKDLNIYVTTAS 240

Query: 241 NAQESSFGTYCPGMQPAPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQYQTVKSRTS 300
           NAQESSFGTYCPGM+PAPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQY+TVK RTS
Sbjct: 241 NAQESSFGTYCPGMEPAPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQYRTVKERTS 300

Query: 301 NPNNLNAGSHVMEYGNSSIKAERLYLYQGFDPASVNLPPYNGRLKMKSMETINQRDADIF 360
           NPNNLN GSHVMEYGNSSIKAERLYLYQGFDPASVNLPPYNGR +MKSM+ INQRDADIF
Sbjct: 301 NPNNLNTGSHVMEYGNSSIKAERLYLYQGFDPASVNLPPYNGRYEMKSMDAINQRDADIF 360

Query: 361 FLWQMYKKFEDGTNERAQILQEIRERVAHRVHLDGSIRLIGFLLFGPEKGPNVLDDVRAP 420
           FLWQMY+KFEDGTNERAQ+L+EIRE V HR HLDGSIR+IGFLLFGPEKG N+LDDVRA 
Sbjct: 361 FLWQMYRKFEDGTNERAQVLEEIRETVTHRTHLDGSIRMIGFLLFGPEKGSNILDDVRAS 420

Query: 421 GLPLVDDWECLKSMVRVLESYCGSLTQYGMKHMRAIANICNRGVSEASMREASMAACNGG 474
           GLPLVDDWECLKSMVRVLESYCGSLTQYGMKHMRAIANICNRGVS+ASMREASM ACNGG
Sbjct: 421 GLPLVDDWECLKSMVRVLESYCGSLTQYGMKHMRAIANICNRGVSKASMREASMVACNGG 480

[Close]

BLAST of Bhi12G000375 vs. NCBI nr
Match: XP_023550713.1 (legumain [Cucurbita pepo subsp. pepo])

HSP 1 Score: 836.6 bits (2160), Expect = 1.0e-238
Identity = 412/496 (83.06%), Postives = 436/496 (87.90%), Query Frame = 0


Query: 1   MAASLTSNLLLLLLLFLAYGGANASPWDRWERTIRMPTEKEELGGGGDD-DDDQKVGTRW 60
           MA+  TS+LL LLLLFLAYGGA A PWDRWER IRMPTEK EL    DD  DDQK+GTRW
Sbjct: 1   MASPSTSSLLFLLLLFLAYGGAKAVPWDRWERIIRMPTEKGELVDDDDDAADDQKLGTRW 60

Query: 61  AVLVAGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIASNVLNPRPGIIINHP 120
           AVLVAGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIA NVLNPRPGIIINHP
Sbjct: 61  AVLVAGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIAGNVLNPRPGIIINHP 120

Query: 121 QGEDVYAGVPKDYTGEHVTAQNLYAVLLGNKTAVNGGSGKVVDSKPNDRIFVYYSDHGGP 180
           QG+DVYAGVPKDYTGE VTAQNLYAVLLGNKTAV GGSGKVVDSKPNDRIFVYYSDHGGP
Sbjct: 121 QGQDVYAGVPKDYTGEQVTAQNLYAVLLGNKTAVTGGSGKVVDSKPNDRIFVYYSDHGGP 180

Query: 181 GVLGL---------------------FSRTRLVIYVEACESGSIFEGIMPKDLNIYVTTA 240
           GVLG+                          +VIYVEACESGSIFEG+MPKDLNIYVTTA
Sbjct: 181 GVLGMPNLPFVYAMDFIEVLKKKHAAKGYKEMVIYVEACESGSIFEGVMPKDLNIYVTTA 240

Query: 241 SNAQESSFGTYCPGMQPAPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQYQTVKSRT 300
           SNA+ESSFGTYCPGMQP PPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQY+TVK RT
Sbjct: 241 SNAEESSFGTYCPGMQPPPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQYETVKKRT 300

Query: 301 SNPNNLNAGSHVMEYGNSSIKAERLYLYQGFDPASVNLPPYNGRLKMKSMETINQRDADI 360
           ++PN+LNAGSHVMEYGNSSIK ERLYLYQGFDPASVNLPPYNG L+MK+METINQRDADI
Sbjct: 301 ASPNDLNAGSHVMEYGNSSIKVERLYLYQGFDPASVNLPPYNGGLEMKTMETINQRDADI 360

Query: 361 FFLWQMYKKFEDGTNERAQILQEIRERVAHRVHLDGSIRLIGFLLFGPEKGPNVLDDVRA 420
           FFLWQMY+K E+GTNERA++L+EIRE +AHR HLDGSIR+IGFLLFGPE   NVLD VRA
Sbjct: 361 FFLWQMYRKLEEGTNERAEVLEEIRETIAHRAHLDGSIRMIGFLLFGPESVSNVLDHVRA 420

Query: 421 PGLPLVDDWECLKSMVRVLESYCGSLTQYGMKHMRAIANICNRGVSEASMREASMAACNG 474
            GLPLVDDW CLKSMVRVLE +CGSLTQYGMKHMRAIANICN+GVSEASMREASMAACNG
Sbjct: 421 SGLPLVDDWTCLKSMVRVLEEHCGSLTQYGMKHMRAIANICNKGVSEASMREASMAACNG 480

[Close]

BLAST of Bhi12G000375 vs. ExPASy TrEMBL
Match: A0A5A7TDF7 (Legumain OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold558G00120 PE=3 SV=1)

HSP 1 Score: 894.4 bits (2310), Expect = 2.0e-256
Identity = 437/495 (88.28%), Postives = 453/495 (91.52%), Query Frame = 0


Query: 1   MAASLTSNLLLLLLLFLAYGGANASPWDRWERTIRMPTEKEELGGGGDDDDDQKVGTRWA 60
           MAA  TSNL+LLLLL LAYGGA+ASPWDRWERTIRMPTEKEE+GG G DDDD+KVGTRWA
Sbjct: 1   MAAPSTSNLVLLLLLLLAYGGAHASPWDRWERTIRMPTEKEEVGGSGGDDDDRKVGTRWA 60

Query: 61  VLVAGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIASNVLNPRPGIIINHPQ 120
           VLVAGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIA+NVLNPRPGIIINHPQ
Sbjct: 61  VLVAGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIATNVLNPRPGIIINHPQ 120

Query: 121 GEDVYAGVPKDYTGEHVTAQNLYAVLLGNKTAVNGGSGKVVDSKPNDRIFVYYSDHGGPG 180
           GEDVYAGVPKDYTGEHVTAQNLYAVLLGN+TAVNGGSGKVVDSKPNDRIFVYYSDHGGPG
Sbjct: 121 GEDVYAGVPKDYTGEHVTAQNLYAVLLGNRTAVNGGSGKVVDSKPNDRIFVYYSDHGGPG 180

Query: 181 VLGL---------------------FSRTRLVIYVEACESGSIFEGIMPKDLNIYVTTAS 240
           VLG+                          +VIYVEACESGSIFEGIMP+DLNIYVTTAS
Sbjct: 181 VLGMPNLPFVYAMDFIEVLKKKHAAKGYKEMVIYVEACESGSIFEGIMPRDLNIYVTTAS 240

Query: 241 NAQESSFGTYCPGMQPAPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQYQTVKSRTS 300
           NAQESSFGTYCPGMQPAPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQYQTVK RTS
Sbjct: 241 NAQESSFGTYCPGMQPAPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQYQTVKERTS 300

Query: 301 NPNNLNAGSHVMEYGNSSIKAERLYLYQGFDPASVNLPPYNGRLKMKSMETINQRDADIF 360
           NPNNLN GSHVMEYGNSSIKAERLYLYQGFDPASVNLPPYNGRL+MKSME INQRDADIF
Sbjct: 301 NPNNLNTGSHVMEYGNSSIKAERLYLYQGFDPASVNLPPYNGRLEMKSMEAINQRDADIF 360

Query: 361 FLWQMYKKFEDGTNERAQILQEIRERVAHRVHLDGSIRLIGFLLFGPEKGPNVLDDVRAP 420
           FLWQMY+KFEDGTNE+AQIL+EIRE V HR HLDGSIR+IGFLLFGPEKG NVLDDVRA 
Sbjct: 361 FLWQMYRKFEDGTNEKAQILEEIRETVTHRSHLDGSIRMIGFLLFGPEKGSNVLDDVRAS 420

Query: 421 GLPLVDDWECLKSMVRVLESYCGSLTQYGMKHMRAIANICNRGVSEASMREASMAACN-G 474
           GLPLVDDWECLKSMVRVLESYCGSLTQYGMKHMRA+ANICNRGVSEASMREASMAACN G
Sbjct: 421 GLPLVDDWECLKSMVRVLESYCGSLTQYGMKHMRALANICNRGVSEASMREASMAACNGG 480

[Close]

BLAST of Bhi12G000375 vs. ExPASy TrEMBL
Match: A0A1S3ATV0 (legumain OS=Cucumis melo OX=3656 GN=LOC103482645 PE=3 SV=1)

HSP 1 Score: 894.0 bits (2309), Expect = 2.6e-256
Identity = 437/495 (88.28%), Postives = 453/495 (91.52%), Query Frame = 0


Query: 1   MAASLTSNLLLLLLLFLAYGGANASPWDRWERTIRMPTEKEELGGGGDDDDDQKVGTRWA 60
           MAA  TSNL+LLLLL LAYGGA+ASPWDRWERTIRMPTE+EE+GG G DDDD+KVGTRWA
Sbjct: 1   MAAPSTSNLVLLLLLLLAYGGAHASPWDRWERTIRMPTEEEEVGGSGGDDDDRKVGTRWA 60

Query: 61  VLVAGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIASNVLNPRPGIIINHPQ 120
           VLVAGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIA+NVLNPRPGIIINHPQ
Sbjct: 61  VLVAGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIATNVLNPRPGIIINHPQ 120

Query: 121 GEDVYAGVPKDYTGEHVTAQNLYAVLLGNKTAVNGGSGKVVDSKPNDRIFVYYSDHGGPG 180
           GEDVYAGVPKDYTGEHVTAQNLYAVLLGN+TAVNGGSGKVVDSKPNDRIFVYYSDHGGPG
Sbjct: 121 GEDVYAGVPKDYTGEHVTAQNLYAVLLGNRTAVNGGSGKVVDSKPNDRIFVYYSDHGGPG 180

Query: 181 VLGL---------------------FSRTRLVIYVEACESGSIFEGIMPKDLNIYVTTAS 240
           VLG+                          +VIYVEACESGSIFEGIMPKDLNIYVTTAS
Sbjct: 181 VLGMPNLPFVYAMDFIEVLKKKHAAKGYKEMVIYVEACESGSIFEGIMPKDLNIYVTTAS 240

Query: 241 NAQESSFGTYCPGMQPAPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQYQTVKSRTS 300
           NAQESSFGTYCPGMQPAPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQYQTVK RTS
Sbjct: 241 NAQESSFGTYCPGMQPAPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQYQTVKERTS 300

Query: 301 NPNNLNAGSHVMEYGNSSIKAERLYLYQGFDPASVNLPPYNGRLKMKSMETINQRDADIF 360
           NPNNLN GSHVMEYGNSSIKAERLYLYQGFDPASVNLPPYNGRL+MKSME INQRDADIF
Sbjct: 301 NPNNLNTGSHVMEYGNSSIKAERLYLYQGFDPASVNLPPYNGRLEMKSMEAINQRDADIF 360

Query: 361 FLWQMYKKFEDGTNERAQILQEIRERVAHRVHLDGSIRLIGFLLFGPEKGPNVLDDVRAP 420
           FLWQMY+KFEDGTNE+AQIL+EIRE V HR HLDGSIR+IGFLLFGPEKG NVLDDVRA 
Sbjct: 361 FLWQMYRKFEDGTNEKAQILEEIRETVTHRSHLDGSIRMIGFLLFGPEKGSNVLDDVRAS 420

Query: 421 GLPLVDDWECLKSMVRVLESYCGSLTQYGMKHMRAIANICNRGVSEASMREASMAACN-G 474
           GLPLVDDWECLKSMVRVLESYCGSLTQYGMKHMRA+ANICNRGVSEASMREASMAACN G
Sbjct: 421 GLPLVDDWECLKSMVRVLESYCGSLTQYGMKHMRALANICNRGVSEASMREASMAACNGG 480

[Close]

BLAST of Bhi12G000375 vs. ExPASy TrEMBL
Match: A0A0A0KMT9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G160170 PE=3 SV=1)

HSP 1 Score: 876.3 bits (2263), Expect = 5.7e-251
Identity = 425/494 (86.03%), Postives = 448/494 (90.69%), Query Frame = 0


Query: 1   MAASLTSNLLLLLLLFLAYGGANASPWDRWERTIRMPTEKEELGGGGDDDDDQKVGTRWA 60
           MA+  TSNL+L LLLFLAYG A+ASPWDRWERTIRMPTEKEE+GG G    D+KVGTRWA
Sbjct: 1   MASPSTSNLVLFLLLFLAYGCADASPWDRWERTIRMPTEKEEMGGAG----DRKVGTRWA 60

Query: 61  VLVAGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIASNVLNPRPGIIINHPQ 120
           VL+AGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIA+NVLNPRPGIIINHPQ
Sbjct: 61  VLIAGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIATNVLNPRPGIIINHPQ 120

Query: 121 GEDVYAGVPKDYTGEHVTAQNLYAVLLGNKTAVNGGSGKVVDSKPNDRIFVYYSDHGGPG 180
           GEDVYAGVPKDYTGEHVTAQNLYAVLLGN+TAV+GGSGKVVDSKPNDRIFVYYSDHGGPG
Sbjct: 121 GEDVYAGVPKDYTGEHVTAQNLYAVLLGNRTAVDGGSGKVVDSKPNDRIFVYYSDHGGPG 180

Query: 181 VLGL---------------------FSRTRLVIYVEACESGSIFEGIMPKDLNIYVTTAS 240
           VLG+                          +VIYVEACESGSIFEGI+PKDLNIYVTTAS
Sbjct: 181 VLGMPNLPFVYAMDFIEVLKKKHAAKGYKEMVIYVEACESGSIFEGILPKDLNIYVTTAS 240

Query: 241 NAQESSFGTYCPGMQPAPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQYQTVKSRTS 300
           NAQESSFGTYCPGM+PAPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQY+TVK RTS
Sbjct: 241 NAQESSFGTYCPGMEPAPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQYRTVKERTS 300

Query: 301 NPNNLNAGSHVMEYGNSSIKAERLYLYQGFDPASVNLPPYNGRLKMKSMETINQRDADIF 360
           NPNNLN GSHVMEYGNSSIKAERLYLYQGFDPASVNLPPYNGR +MKSM+ INQRDADIF
Sbjct: 301 NPNNLNTGSHVMEYGNSSIKAERLYLYQGFDPASVNLPPYNGRYEMKSMDAINQRDADIF 360

Query: 361 FLWQMYKKFEDGTNERAQILQEIRERVAHRVHLDGSIRLIGFLLFGPEKGPNVLDDVRAP 420
           FLWQMY+KFEDGTNERAQ+L+EIRE V HR HLDGSIR+IGFLLFGPEKG N+LDDVRA 
Sbjct: 361 FLWQMYRKFEDGTNERAQVLEEIRETVTHRTHLDGSIRMIGFLLFGPEKGSNILDDVRAS 420

Query: 421 GLPLVDDWECLKSMVRVLESYCGSLTQYGMKHMRAIANICNRGVSEASMREASMAACNGG 474
           GLPLVDDWECLKSMVRVLESYCGSLTQYGMKHMRAIANICNRGVS+ASMREASM ACNGG
Sbjct: 421 GLPLVDDWECLKSMVRVLESYCGSLTQYGMKHMRAIANICNRGVSKASMREASMVACNGG 480

[Close]

BLAST of Bhi12G000375 vs. ExPASy TrEMBL
Match: A0A6J1E2K0 (legumain OS=Cucurbita moschata OX=3662 GN=LOC111430261 PE=3 SV=1)

HSP 1 Score: 835.1 bits (2156), Expect = 1.5e-238
Identity = 412/497 (82.90%), Postives = 437/497 (87.93%), Query Frame = 0


Query: 1   MAASLTSNLLLLLLLFLAYGGANASPWDRWERTIRMPTEKEELGGGGDD-DDDQKVGTRW 60
           MA+S TS+LLL LLLFLAYGGA A PW+RWERTIRMPTEKEEL    DD  DDQK+GTRW
Sbjct: 1   MASSSTSSLLLFLLLFLAYGGAKAVPWNRWERTIRMPTEKEELVDDDDDAADDQKLGTRW 60

Query: 61  AVLVAGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIASNVLNPRPGIIINHP 120
           AVLVAGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIA NVLNPRPGIIINHP
Sbjct: 61  AVLVAGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIAGNVLNPRPGIIINHP 120

Query: 121 QGEDVYAGVPKDYTGEHVTAQNLYAVLLGNKTAVNGGSGKVVDSKPNDRIFVYYSDHGGP 180
           QG+DVYAGVPKDYTGE VTAQNLYAVLLGNKTAV GGSGKVVDSKPNDRIFVYYSDHGGP
Sbjct: 121 QGQDVYAGVPKDYTGEQVTAQNLYAVLLGNKTAVTGGSGKVVDSKPNDRIFVYYSDHGGP 180

Query: 181 GVLGL---------------------FSRTRLVIYVEACESGSIFEGIMPKDLNIYVTTA 240
           GVLG+                          +VIYVEACESGSIFEG+MPKDLNIYVTTA
Sbjct: 181 GVLGMPNLPFVYAMDFIEVLKKKHAAKGYKEMVIYVEACESGSIFEGVMPKDLNIYVTTA 240

Query: 241 SNAQESSFGTYCPGMQPAPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQYQTVKSRT 300
           SNA+ESSFGTYCPGMQP PPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQY+TVK RT
Sbjct: 241 SNAEESSFGTYCPGMQPPPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQYETVKKRT 300

Query: 301 SNPNNLNAGSHVMEYGNSSIKAERLYLYQGFDPASVNLPPYNGRLKMKSMETINQRDADI 360
           ++PN+LNAGSHVMEYGNSSIK ERLYLYQGFDPASVNLPPYNG L+MK+METINQRDADI
Sbjct: 301 ASPNDLNAGSHVMEYGNSSIKTERLYLYQGFDPASVNLPPYNGGLEMKTMETINQRDADI 360

Query: 361 FFLWQMYKKFEDGTNERAQILQEIRERVAHRVHLDGSIRLIGFLLFGPEKGPNVLDDVRA 420
           FFLWQMY+K E+GTNERA++L+EIRE VAHR HLDGSIR+IGFLLFGPE   NVLD VR 
Sbjct: 361 FFLWQMYRKLEEGTNERAEVLEEIRETVAHRAHLDGSIRMIGFLLFGPENVSNVLDHVRP 420

Query: 421 PGLPLVDDWECLKSMVRVLESYCGSLTQYGMKHMRAIANICNRGVSEASMREASMAACNG 474
            GLPLVDDW CLKSMVRVLE +CGSLTQYGMKHMRAIANICN+GVSEASMREASMAACNG
Sbjct: 421 SGLPLVDDWTCLKSMVRVLEEHCGSLTQYGMKHMRAIANICNKGVSEASMREASMAACNG 480

[Close]

BLAST of Bhi12G000375 vs. ExPASy TrEMBL
Match: A0A6J1HZ01 (legumain OS=Cucurbita maxima OX=3661 GN=LOC111468940 PE=3 SV=1)

HSP 1 Score: 827.0 bits (2135), Expect = 4.0e-236
Identity = 408/500 (81.60%), Postives = 438/500 (87.60%), Query Frame = 0


Query: 1   MAASLTSNLLLLLLLFLAYGGANASPWDRWERTIRMPTEKEELGGGGDDD----DDQKVG 60
           MA+  TS+LLLLLLLFLAY GA A PWDRWERTIRMPTEK EL    DDD    DD+K+G
Sbjct: 1   MASPSTSSLLLLLLLFLAYSGARAVPWDRWERTIRMPTEKGEL---VDDDAAAADDKKLG 60

Query: 61  TRWAVLVAGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIASNVLNPRPGIII 120
           TRWAVLVAGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIA NVLNPRPGIII
Sbjct: 61  TRWAVLVAGSSGFGNYRHQADICHAYQLLKKGGLKDENIVVFMYDDIAGNVLNPRPGIII 120

Query: 121 NHPQGEDVYAGVPKDYTGEHVTAQNLYAVLLGNKTAVNGGSGKVVDSKPNDRIFVYYSDH 180
           NHPQG++VY GVPKDYTGE VTAQNLYAVLLGNKTAV GGSGKVVDSKPNDRIFVYYSDH
Sbjct: 121 NHPQGQNVYVGVPKDYTGEQVTAQNLYAVLLGNKTAVTGGSGKVVDSKPNDRIFVYYSDH 180

Query: 181 GGPGVLGL---------------------FSRTRLVIYVEACESGSIFEGIMPKDLNIYV 240
           GGPGVLG+                          +VIYVEACESGSIFEG+MPKDLNIYV
Sbjct: 181 GGPGVLGMPNLPFVYAMDFIEVLKKKHAAKGYKEMVIYVEACESGSIFEGVMPKDLNIYV 240

Query: 241 TTASNAQESSFGTYCPGMQPAPPPEYMTCLGDLYSVAWMEDSETHNLKRETIDQQYQTVK 300
           TTASNA+ESSFGTYCPGMQP PPP+YMTCLGDLYSVAWMEDSETHNLKRETIDQQY+TVK
Sbjct: 241 TTASNAEESSFGTYCPGMQPPPPPDYMTCLGDLYSVAWMEDSETHNLKRETIDQQYETVK 300

Query: 301 SRTSNPNNLNAGSHVMEYGNSSIKAERLYLYQGFDPASVNLPPYNGRLKMKSMETINQRD 360
            RT++PN+LNAGSHVMEYGNSSIKAERLYLYQGFDPASVNLPPYNG L+MK+METINQRD
Sbjct: 301 KRTASPNDLNAGSHVMEYGNSSIKAERLYLYQGFDPASVNLPPYNGGLEMKTMETINQRD 360

Query: 361 ADIFFLWQMYKKFEDGTNERAQILQEIRERVAHRVHLDGSIRLIGFLLFGPEKGPNVLDD 420
           ADIFFLWQMY+K E+GTNERA++L+EIRE +AHR HLDGSIR+IGFLLFGPE   NV+D 
Sbjct: 361 ADIFFLWQMYRKLEEGTNERAEVLEEIRETIAHRAHLDGSIRMIGFLLFGPENVSNVIDH 420

Query: 421 VRAPGLPLVDDWECLKSMVRVLESYCGSLTQYGMKHMRAIANICNRGVSEASMREASMAA 474
           VRA GLPLVDDW+CLKSMVRVLE +CGSLTQYGMKHMRAIANICN+GVSEASMREASMAA
Sbjct: 421 VRASGLPLVDDWKCLKSMVRVLEEHCGSLTQYGMKHMRAIANICNKGVSEASMREASMAA 480

The following BLAST results are available for this feature:

BLAST of Bhi12G000375 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G62710.1	4.7e-181	65.73	beta vacuolar processing enzyme	[more]
AT4G32940.1	8.3e-154	54.73	gamma vacuolar processing enzyme	[more]
AT2G25940.1	5.8e-147	55.06	alpha-vacuolar processing enzyme	[more]
AT3G20210.1	1.1e-110	46.30	delta vacuolar processing enzyme	[more]
AT3G20210.2	4.3e-110	46.30	delta vacuolar processing enzyme	[more]

BLAST of Bhi12G000375 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P49042	6.8e-193	67.84	Vacuolar-processing enzyme OS=Ricinus communis OX=3988 PE=1 SV=1	[more]
P49045	1.2e-189	65.23	Vacuolar-processing enzyme OS=Glycine max OX=3847 PE=2 SV=1	[more]
P49046	1.2e-186	64.20	Legumain OS=Canavalia ensiformis OX=3823 PE=1 SV=1	[more]
O24326	2.9e-183	64.60	Vacuolar-processing enzyme OS=Phaseolus vulgaris OX=3885 PE=2 SV=1	[more]
Q39044	6.6e-180	65.73	Vacuolar-processing enzyme beta-isozyme OS=Arabidopsis thaliana OX=3702 GN=bVPE ...	[more]

BLAST of Bhi12G000375 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038907005.1	9.5e-277	95.56	LOW QUALITY PROTEIN: vacuolar-processing enzyme-like [Benincasa hispida]	[more]
KAA0039681.1	4.2e-256	88.28	legumain [Cucumis melo var. makuwa]	[more]
XP_008437131.1	5.4e-256	88.28	PREDICTED: legumain [Cucumis melo]	[more]
XP_004147613.1	1.2e-250	86.03	legumain [Cucumis sativus] >KGN50214.1 hypothetical protein Csa_000360 [Cucumis ...	[more]
XP_023550713.1	1.0e-238	83.06	legumain [Cucurbita pepo subsp. pepo]	[more]

BLAST of Bhi12G000375 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TDF7	2.0e-256	88.28	Legumain OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold558G00120 PE=3 ...	[more]
A0A1S3ATV0	2.6e-256	88.28	legumain OS=Cucumis melo OX=3656 GN=LOC103482645 PE=3 SV=1	[more]
A0A0A0KMT9	5.7e-251	86.03	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G160170 PE=3 SV=1	[more]
A0A6J1E2K0	1.5e-238	82.90	legumain OS=Cucurbita moschata OX=3662 GN=LOC111430261 PE=3 SV=1	[more]
A0A6J1HZ01	4.0e-236	81.60	legumain OS=Cucurbita maxima OX=3661 GN=LOC111468940 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001096	Peptidase C13, legumain	PRINTS	PR00776	HEMOGLOBNASE	coord: 131..146 score: 59.78 coord: 84..113 score: 65.51 coord: 159..178 score: 63.48 coord: 59..83 score: 75.3
IPR001096	Peptidase C13, legumain	PFAM	PF01650	Peptidase_C13	coord: 58..183 e-value: 3.0E-56 score: 190.8 coord: 189..308 e-value: 9.2E-38 score: 130.2
IPR001096	Peptidase C13, legumain	PIRSF	PIRSF019663	Legumain	coord: 185..473 e-value: 1.1E-118 score: 394.9 coord: 3..190 e-value: 7.9E-85 score: 283.2
IPR001096	Peptidase C13, legumain	PANTHER	PTHR12000	HEMOGLOBINASE FAMILY MEMBER	coord: 13..185
IPR001096	Peptidase C13, legumain	PANTHER	PTHR12000	HEMOGLOBINASE FAMILY MEMBER	coord: 187..458
IPR043577	Asparaginyl endopeptidase	PIRSF	PIRSF500139	AE	coord: 3..190 e-value: 1.5E-87 score: 292.3 coord: 185..473 e-value: 2.4E-119 score: 397.2
None	No IPR available	GENE3D	1.10.132.130		coord: 333..464 e-value: 4.7E-36 score: 125.7
None	No IPR available	GENE3D	3.40.50.1460		coord: 56..331 e-value: 4.1E-109 score: 366.4
None	No IPR available	PROSITE	PS50152	25A_SYNTH_3	coord: 335..365 score: 8.650909
IPR033165	Legumain beta	PANTHER	PTHR12000:SF25	VACUOLAR-PROCESSING ENZYME BETA-ISOZYME 1	coord: 187..458 coord: 13..185

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi12M000375	Bhi12M000375	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051603	proteolysis involved in cellular protein catabolic process
biological_process	GO:0006624	vacuolar protein processing
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005773	vacuole
molecular_function	GO:0004197	cysteine-type endopeptidase activity
molecular_function	GO:0008233	peptidase activity