Bhi12G000369 (gene) Wax gourd (B227) v1

Overview


Name	Bhi12G000369
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	cytochrome P450, family 706, subfamily A, polypeptide 3
Location	chr12: 13261312 .. 13262307 (-)
RNA-Seq Expression	Bhi12G000369
Synteny	Bhi12G000369

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAACTCACTTTCAATTTCCTTCTAACTCTTAATTTCTTTAATCCCAATCACCAAACCTGCTTTCTTTTCACCCTCCTTGCCGCTTTTCTACTGATTATTTTTTATGTCAAACTTTCTCGACGGCGCTTGCAGCTTCCACCAGGTCCTCACGGCGTCCCATTGCTCGGGAATTTGCCGTTTCTAGACCCAGAACTTCACACTTACTTCACAAAATTGGGCAAAAAATATGGCTCAATTGTGAAGCTGCAGTTGGGTAGAAAGATTTGTATAATCGTCAATTCACCATCGGTGGCACATGAGGTGTTAAAAGTCCATGATATTACATTTGCCAATCGCGATGTTCCCCAAGCAGGTCGAGTCGCAACGTATGGCGGTTTTGACATAACTTGGACCCCATACGGACCCGAGTGGCGGATGTTGAGAAAAGTTTGTGCTATCAAGATGCTTAGCAACGCTAGTTTGGACACTGTCTACGAGCTCCGCCGTAGAGAGGTTAGAAACACCGTGGCTCAGTTGTACCCGCGAGCCGGGTCGGTGGTGAATGTGGGAGAGCAGGGTTTCTTGACGGTTTTCAACGTGATTACGAGCATGTTGTGGGGTGGGTCAACACTGGAAGGAAATCAAAAGGAGGCCGTTGCGGCTGAGTTTAGGAAGATGGTTTCGGACATGACTGAGTTAGTAGCCAAGCCTAATATTTCTGACTTTTTTCCATGCTTGGCAGGTTTGGATCTCCAAGGAATTGAGAAGCAAATGCTTAAACTTGTTACGAAATTGGACTCCATCTTTGACAAGATGATTGATGAACGATTGAGAAAGGCGGATGAGGAGGAAAATAGGGATAAAAATGATTTCTTGCAGTTCTTACTTAAAGTCAAAGATGAAAGCGATTCCAAAACACCTCTGACAATGGTCCAACTTAAAGCCTTGCTAATGGTAACTACTTCCGACTTTCAATCAATATCTATAATGTTTTATGTTCATTATAATATTTAA

mRNA sequence

ATGGAACTCACTTTCAATTTCCTTCTAACTCTTAATTTCTTTAATCCCAATCACCAAACCTGCTTTCTTTTCACCCTCCTTGCCGCTTTTCTACTGATTATTTTTTATGTCAAACTTTCTCGACGGCGCTTGCAGCTTCCACCAGGTCCTCACGGCGTCCCATTGCTCGGGAATTTGCCGTTTCTAGACCCAGAACTTCACACTTACTTCACAAAATTGGGCAAAAAATATGGCTCAATTGTGAAGCTGCAGTTGGGTAGAAAGATTTGTATAATCGTCAATTCACCATCGGTGGCACATGAGGTGTTAAAAGTCCATGATATTACATTTGCCAATCGCGATGTTCCCCAAGCAGGTCGAGTCGCAACGTATGGCGGTTTTGACATAACTTGGACCCCATACGGACCCGAGTGGCGGATGTTGAGAAAAGTTTGTGCTATCAAGATGCTTAGCAACGCTAGTTTGGACACTGTCTACGAGCTCCGCCGTAGAGAGGTTAGAAACACCGTGGCTCAGTTGTACCCGCGAGCCGGGTCGGTGGTGAATGTGGGAGAGCAGGGTTTCTTGACGGTTTTCAACGTGATTACGAGCATGTTGTGGGGTGGGTCAACACTGGAAGGAAATCAAAAGGAGGCCGTTGCGGCTGAGTTTAGGAAGATGGTTTCGGACATGACTGAGTTAGTAGCCAAGCCTAATATTTCTGACTTTTTTCCATGCTTGGCAGGTTTGGATCTCCAAGGAATTGAGAAGCAAATGCTTAAACTTGTTACGAAATTGGACTCCATCTTTGACAAGATGATTGATGAACGATTGAGAAAGGCGGATGAGGAGGAAAATAGGGATAAAAATGATTTCTTGCAGTTCTTACTTAAAGTCAAAGATGAAAGCGATTCCAAAACACCTCTGACAATGGTCCAACTTAAAGCCTTGCTAATGGTAACTACTTCCGACTTTCAATCAATATCTATAATGTTTTATGTTCATTATAATATTTAA

Coding sequence (CDS)

ATGGAACTCACTTTCAATTTCCTTCTAACTCTTAATTTCTTTAATCCCAATCACCAAACCTGCTTTCTTTTCACCCTCCTTGCCGCTTTTCTACTGATTATTTTTTATGTCAAACTTTCTCGACGGCGCTTGCAGCTTCCACCAGGTCCTCACGGCGTCCCATTGCTCGGGAATTTGCCGTTTCTAGACCCAGAACTTCACACTTACTTCACAAAATTGGGCAAAAAATATGGCTCAATTGTGAAGCTGCAGTTGGGTAGAAAGATTTGTATAATCGTCAATTCACCATCGGTGGCACATGAGGTGTTAAAAGTCCATGATATTACATTTGCCAATCGCGATGTTCCCCAAGCAGGTCGAGTCGCAACGTATGGCGGTTTTGACATAACTTGGACCCCATACGGACCCGAGTGGCGGATGTTGAGAAAAGTTTGTGCTATCAAGATGCTTAGCAACGCTAGTTTGGACACTGTCTACGAGCTCCGCCGTAGAGAGGTTAGAAACACCGTGGCTCAGTTGTACCCGCGAGCCGGGTCGGTGGTGAATGTGGGAGAGCAGGGTTTCTTGACGGTTTTCAACGTGATTACGAGCATGTTGTGGGGTGGGTCAACACTGGAAGGAAATCAAAAGGAGGCCGTTGCGGCTGAGTTTAGGAAGATGGTTTCGGACATGACTGAGTTAGTAGCCAAGCCTAATATTTCTGACTTTTTTCCATGCTTGGCAGGTTTGGATCTCCAAGGAATTGAGAAGCAAATGCTTAAACTTGTTACGAAATTGGACTCCATCTTTGACAAGATGATTGATGAACGATTGAGAAAGGCGGATGAGGAGGAAAATAGGGATAAAAATGATTTCTTGCAGTTCTTACTTAAAGTCAAAGATGAAAGCGATTCCAAAACACCTCTGACAATGGTCCAACTTAAAGCCTTGCTAATGGTAACTACTTCCGACTTTCAATCAATATCTATAATGTTTTATGTTCATTATAATATTTAA

Protein sequence

MELTFNFLLTLNFFNPNHQTCFLFTLLAAFLLIIFYVKLSRRRLQLPPGPHGVPLLGNLPFLDPELHTYFTKLGKKYGSIVKLQLGRKICIIVNSPSVAHEVLKVHDITFANRDVPQAGRVATYGGFDITWTPYGPEWRMLRKVCAIKMLSNASLDTVYELRRREVRNTVAQLYPRAGSVVNVGEQGFLTVFNVITSMLWGGSTLEGNQKEAVAAEFRKMVSDMTELVAKPNISDFFPCLAGLDLQGIEKQMLKLVTKLDSIFDKMIDERLRKADEEENRDKNDFLQFLLKVKDESDSKTPLTMVQLKALLMVTTSDFQSISIMFYVHYNI

Homology

[Close]

BLAST of Bhi12G000369 vs. TAIR 10
Match: AT4G12300.1 (cytochrome P450, family 706, subfamily A, polypeptide 4 )

HSP 1 Score: 295.4 bits (755), Expect = 5.6e-80
Identity = 150/302 (49.67%), Postives = 209/302 (69.21%), Query Frame = 0


Query: 15  NPNHQTCFLFTLLAAFLLIIFYVKLSRRRLQLPPGPHGVPLLGNLPFLDPELHTYFTKLG 74
           N  + T +   +L     I++Y+     +  LPPGP G+P++GNLPFLDP+LHTYF  L 
Sbjct: 11  NTINLTPYAIVILTTVFSILWYIFKRSPQPSLPPGPRGLPIVGNLPFLDPDLHTYFANLA 70

Query: 75  KKYGSIVKLQLGRKICIIVNSPSVAHEVLKVHDITFANRDVPQAGRVATYGGFDITWTPY 134
           + +G I KL LG K+ I+VNSPS+A E+LK  DI F+NRDVP  GR ATYGG DI WTPY
Sbjct: 71  QSHGPIFKLNLGSKLTIVVNSPSLAREILKDQDINFSNRDVPLTGRAATYGGIDIVWTPY 130

Query: 135 GPEWRMLRKVCAIKMLSNASLDTVYELRRREVRNTVAQLYP--RAGSVVNVGEQGFLTVF 194
           G EWR LRK+C +K+LS  +LD+ YELRR+EVR     LY   R  S V VG+Q FLT+ 
Sbjct: 131 GAEWRQLRKICVLKLLSRKTLDSFYELRRKEVRERTRYLYEQGRKQSPVKVGDQLFLTMM 190

Query: 195 NVITSMLWGGSTLEGNQKEAVAAEFRKMVSDMTELVAKPNISDFFPCLAGLDLQGIEKQM 254
           N+  +MLWGGS ++  + E+V  EF+ ++S++T L+++PN+SDFFP LA  DLQG+ K+M
Sbjct: 191 NLTMNMLWGGS-VKAEEMESVGTEFKGVISEITRLLSEPNVSDFFPWLARFDLQGLVKRM 250

Query: 255 LKLVTKLDSIFDKMIDER--LRKADEEENRDKNDFLQFLLKVKD-ESDSKTPLTMVQLKA 312
                +LD++ D+ I++   LR  D++E +   DFLQ+L+K+KD E DS+ P+T+  +KA
Sbjct: 251 GVCARELDAVLDRAIEQMKPLRGRDDDEVK---DFLQYLMKLKDQEGDSEVPITINHVKA 308

[Close]

BLAST of Bhi12G000369 vs. TAIR 10
Match: AT4G12320.1 (cytochrome P450, family 706, subfamily A, polypeptide 6 )

HSP 1 Score: 292.7 bits (748), Expect = 3.6e-79
Identity = 145/302 (48.01%), Postives = 212/302 (70.20%), Query Frame = 0


Query: 15  NPNHQTCFLFTLLAAFLLIIFYVKLSRRRLQLPPGPHGVPLLGNLPFLDPELHTYFTKLG 74
           N  + T +   +L A   I++Y+     +  LPPGP G+P++GNLPFLDP+LHTYFTKL 
Sbjct: 11  NTINLTPYAILILIAIFSILWYLFKRSPQPHLPPGPRGLPIVGNLPFLDPDLHTYFTKLA 70

Query: 75  KKYGSIVKLQLGRKICIIVNSPSVAHEVLKVHDITFANRDVPQAGRVATYGGFDITWTPY 134
           + YG I KL LG K+ ++VN+PS+A E+LK  DI F+N DVP   R  TYGG D+ W PY
Sbjct: 71  ESYGPIFKLNLGSKLTVVVNTPSLAREILKDQDINFSNHDVPLTARAVTYGGLDLVWLPY 130

Query: 135 GPEWRMLRKVCAIKMLSNASLDTVYELRRREVRNTVAQLYPRA--GSVVNVGEQGFLTVF 194
           G EWRMLRKVC +K+LS+ +L++ YELRR+E+R     LY +    S VNVGEQ FLT+ 
Sbjct: 131 GAEWRMLRKVCVLKLLSHRTLNSFYELRRKEIRERTRYLYQKGQEESPVNVGEQVFLTMM 190

Query: 195 NVITSMLWGGSTLEGNQKEAVAAEFRKMVSDMTELVAKPNISDFFPCLAGLDLQGIEKQM 254
           N+  +MLWGGS ++  + E+V  EF++++S++T L+ +PN+SDFFP LA  DLQG+ K+M
Sbjct: 191 NLTMNMLWGGS-VKAEEMESVGTEFKEVISEITRLLGEPNVSDFFPRLARFDLQGLVKKM 250

Query: 255 LKLVTKLDSIFDKMIDE-RLRKADEEENRDKNDFLQFLLKVKD-ESDSKTPLTMVQLKAL 313
                +LD+I D+ I++ +L +  + ++ +  DFLQ L+K+KD E+DS+ P+T+  +KA+
Sbjct: 251 HVCARELDAILDRAIEQMQLLRTRDGDDGECKDFLQHLMKLKDQEADSEVPITVNHVKAV 310

[Close]

BLAST of Bhi12G000369 vs. TAIR 10
Match: AT5G44620.1 (cytochrome P450, family 706, subfamily A, polypeptide 3 )

HSP 1 Score: 291.2 bits (744), Expect = 1.1e-78
Identity = 148/279 (53.05%), Postives = 193/279 (69.18%), Query Frame = 0


Query: 36  YVKLSRRRLQLPPGPHGVPLLGNLPFLDPELHTYFTKLGKKYGSIVKLQLGRKICIIVNS 95
           Y K  RR   LPPGP G+P++GNLPFL PELHTYF  L KK+G I KL LG K+ I+V S
Sbjct: 36  YAKCKRRSPPLPPGPWGLPIIGNLPFLQPELHTYFQGLAKKHGPIFKLWLGAKLTIVVTS 95

Query: 96  PSVAHEVLKVHDITFANRDVPQAGRVATYGGFDITWTPYGPEWRMLRKVCAIKMLSNASL 155
             VA E+LK +DI FAN DVP  G V TYGG +I W+PYGP+WRMLRK+C  ++L NA L
Sbjct: 96  SEVAQEILKTNDIIFANHDVPAVGPVNTYGGTEIIWSPYGPKWRMLRKLCVNRILRNAML 155

Query: 156 DTVYELRRREVRNTVAQL--YPRAGSVVNVGEQGFLTVFNVITSMLWGGSTLEGNQKEAV 215
           D+  +LRRRE R TV  L    R GS VN+GEQ FL + NV+T MLW G+T++  ++E V
Sbjct: 156 DSSTDLRRRETRQTVRYLADQARVGSPVNLGEQIFLMMLNVVTQMLW-GTTVKEEEREVV 215

Query: 216 AAEFRKMVSDMTELVAKPNISDFFPCLAGLDLQGIEKQMLKLVTKLDSIFDKMIDERLRK 275
            AEF +++ +M +L+  PNISDFFP L+  DLQG+ K+M +   ++D +FD++I++RL  
Sbjct: 216 GAEFLEVIREMNDLLLVPNISDFFPVLSRFDLQGLAKRMRRPAQRMDQMFDRIINQRLGM 275

Query: 276 ADEEENRDKNDFLQFLLKVKDESDSKTPLTMVQLKALLM 313
             +  +    DFL  LLKVKDE   KT LTM  +KA+LM
Sbjct: 276 DRDSSDGRAVDFLDVLLKVKDEEAEKTKLTMNDVKAVLM 313

[Close]

BLAST of Bhi12G000369 vs. TAIR 10
Match: AT4G12310.1 (cytochrome P450, family 706, subfamily A, polypeptide 5 )

HSP 1 Score: 290.8 bits (743), Expect = 1.4e-78
Identity = 149/292 (51.03%), Postives = 202/292 (69.18%), Query Frame = 0


Query: 26  LLAAFLLIIFYVKLSRRRLQLPPGPHGVPLLGNLPFLDPELHTYFTKLGKKYGSIVKLQL 85
           +L A   I++Y+     +  LPPGP G+P++GNLPFLDP+LHTYFTKL + +G I KL L
Sbjct: 24  ILTATFSILWYIFKRSPQPPLPPGPRGLPIVGNLPFLDPDLHTYFTKLAQSHGPIFKLNL 83

Query: 86  GRKICIIVNSPSVAHEVLKVHDITFANRDVPQAGRVATYGGFDITWTPYGPEWRMLRKVC 145
           G K+ ++VNSPS+A E+LK  DI F+N DVP   R  TYGG D+ W PYG EWRMLRKVC
Sbjct: 84  GSKLTVVVNSPSLASEILKDQDINFSNHDVPLTARAVTYGGLDLVWLPYGAEWRMLRKVC 143

Query: 146 AIKMLSNASLDTVYELRRREVRNTVAQLYPRA--GSVVNVGEQGFLTVFNVITSMLWGGS 205
           A K+ S  +LD+ YELRR+E+R     LY +    S VNVGEQ FLT+ N++ +MLWGGS
Sbjct: 144 AAKLFSRKTLDSFYELRRKEIRERTRCLYQKGLEKSPVNVGEQLFLTMMNLMMNMLWGGS 203

Query: 206 TLEGNQKEAVAAEFRKMVSDMTELVAKPNISDFFPCLAGLDLQGIEKQMLKLVTKLDSIF 265
            ++    E+V  EF+ ++S++T L+  PN+SDFFP LA  DLQG+ K+M      LD+I 
Sbjct: 204 -VKAEDMESVGTEFKGVISEITRLLGVPNVSDFFPMLARFDLQGLVKKMHLYARDLDAIL 263

Query: 266 DKMID--ERLRKADEEENRDKNDFLQFLLKVKD-ESDSKTPLTMVQLKALLM 313
           D+ I+  +RLR  D ++   K DFLQ L+K++D E+DS  P+TM  +KA+LM
Sbjct: 264 DRAIEQMQRLRSRDGDDGECK-DFLQHLMKLRDQEADSDVPITMNHVKAVLM 313

[Close]

BLAST of Bhi12G000369 vs. TAIR 10
Match: AT4G12330.1 (cytochrome P450, family 706, subfamily A, polypeptide 7 )

HSP 1 Score: 274.2 bits (700), Expect = 1.3e-73
Identity = 138/289 (47.75%), Postives = 195/289 (67.47%), Query Frame = 0


Query: 28  AAFLLIIFYVKLSRRRLQLPPGPHGVPLLGNLPFLDPELHTYFTKLGKKYGSIVKLQLGR 87
           + F  I  YVK  R    LPPGP G+P++GNLPFL PELHTYF  L +K+G + KL LG 
Sbjct: 28  SVFWYIWVYVKSKRLFPPLPPGPRGLPIVGNLPFLHPELHTYFHSLAQKHGPVFKLWLGA 87

Query: 88  KICIIVNSPSVAHEVLKVHDITFANRDVPQAGRVATYGGFDITWTPYGPEWRMLRKVCAI 147
           K+ I++ S     ++L+ +D+ FAN DVP AG ++TYGG DI W+PYGPEW MLRK+C  
Sbjct: 88  KLTIVITSSEATRDILRTNDVIFANDDVPVAGSLSTYGGVDIVWSPYGPEWPMLRKICIN 147

Query: 148 KMLSNASLD--TVYELRRREVRNTVAQL--YPRAGSVVNVGEQGFLTVFNVITSMLWGGS 207
           KMLSNA+LD  +   LRR+E R TV  L    RAG  VNVGEQ F+T+ NV+T MLWG +
Sbjct: 148 KMLSNATLDSNSFSALRRQETRRTVRYLADRARAGLAVNVGEQIFVTILNVVTQMLWGET 207

Query: 208 TLEGNQKEAVAAEFRKMVSDMTELVAKPNISDFFPCLAGLDLQGIEKQMLKLVTKLDSIF 267
             +  ++E V AEF ++++++ ++V KPN+SDFFP L+  DLQG+ K++ +   ++D +F
Sbjct: 208 VADDEEREKVGAEFLELITEIIDVVGKPNVSDFFPVLSRFDLQGLAKRVRRSAQRMDRMF 267

Query: 268 DKMIDERLRKADEEENRDKNDFLQFLLKVKDESDSKTPLTMVQLKALLM 313
           D++I +R+   D+    +  DFL  LL  KDE ++   ++M  +KALLM
Sbjct: 268 DRIISQRM-GMDKGSKGNGGDFLMVLLNAKDEDEN---MSMNHVKALLM 312

[Close]

BLAST of Bhi12G000369 vs. ExPASy Swiss-Prot
Match: A0A4D6Q415 (Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN=CYP75B137 PE=1 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 9.4e-40
Identity = 96/291 (32.99%), Postives = 159/291 (54.64%), Query Frame = 0


Query: 26  LLAAFLLIIFYVKLSRRRLQLPPGPHGVPLLGNLPFLDPELHTYFTKLGKKYGSIVKLQL 85
           LL++F++ + Y + S +   LPPGP+G P+LGNLP L  + H     L K+YG + +L+L
Sbjct: 13  LLSSFIVYLLYRRRSAQCPPLPPGPNGWPILGNLPQLGAKPHQTLDALSKQYGPLFRLRL 72

Query: 86  GRKICIIVNSPSVAHEVLKVHDITFANRDVPQAGRVATYGGFDITWTPYGPEWRMLRKVC 145
           G    ++ +S +VA + L+ HD+ F+NR          Y   D+ + PYGP WRMLRK+C
Sbjct: 73  GSVNVVVASSSAVAAQFLRTHDVNFSNRPPNSGAEHVAYNYQDLVFAPYGPRWRMLRKLC 132

Query: 146 AIKMLSNASLDTVYELRRREVRNTVAQLYPRA--GSVVNVGEQGFLTVFNVITSMLWGGS 205
           ++ + S  +LD +  +R+ EV   V  L   A  G +VN+G+   +   N +   + G  
Sbjct: 133 SVHLFSLKALDDLRPVRQGEVACLVRNLRRHADTGVLVNLGKALNVCATNALARAMLGRR 192

Query: 206 TLEGNQKE-AVAAEFRKMVSDMTELVAKPNISDFFPCLAGLDLQGIEKQMLKLVTKLDSI 265
                  + A A EF++MV ++  L    N+ DF P L  LDLQG+  +M +L  + D+ 
Sbjct: 193 VFADEDAQLAEADEFKEMVVELMRLAGVFNVGDFVPGLGWLDLQGVVGKMKRLHRRYDAF 252

Query: 266 FDKMIDERLRKADEEENRDKNDFLQFLLKVKD-ESDSKTPLTMVQLKALLM 313
            D++I+E       + N    D L  L+++K+ +++ +  L    +KALL+
Sbjct: 253 LDRVIEE------NQANAKSGDLLSVLIRLKEADAEGEIKLNNTDIKALLL 297

[Close]

BLAST of Bhi12G000369 vs. ExPASy Swiss-Prot
Match: P37120 (Flavonoid 3',5'-hydroxylase OS=Solanum melongena OX=4111 GN=CYP75A2 PE=2 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 4.4e-37
Identity = 98/295 (33.22%), Postives = 154/295 (52.20%), Query Frame = 0


Query: 28  AAFLLIIFYVKLSR-------RRLQLPPGPHGVPLLGNLPFLDPELHTYFTKLGKKYGSI 87
           A  + II Y+ + +       RR +LPPGP G P++G LP L    H    K+ KKYG I
Sbjct: 11  ATIIYIIVYIIIQKLIATGSWRRRRLPPGPEGWPVIGALPLLGGMPHVALAKMAKKYGPI 70

Query: 88  VKLQLGRKICIIVNSPSVAHEVLKVHDITFANRDVPQAGRV-ATYGGFDITWTPYGPEWR 147
           + L++G    ++ ++P+ A   LK  DI F+NR  P AG     Y   D+ + PYGP W+
Sbjct: 71  MYLKVGTCGMVVASTPNAAKAFLKTLDINFSNRP-PNAGATHMAYNAQDMVFAPYGPRWK 130

Query: 148 MLRKVCAIKMLSNASLDTVYELRRREVRNTVAQLY--PRAGSVVNVGEQGFLTVFNVITS 207
           +LRK+  + ML   +L+    +R  E+ + +  ++     G  + V +     + N+I  
Sbjct: 131 LLRKLSNLHMLGGKALENWANVRANELGHMLKSMFDASHVGERIVVADMLTFAMANMIGQ 190

Query: 208 MLWGGSTLEGNQKEAVAAEFRKMVSDMTELVAKPNISDFFPCLAGLDLQGIEKQMLKLVT 267
           ++          KE    EF+ MV ++  +    NI DF P +A +DLQGIEK M KL  
Sbjct: 191 VMLSKRVFVEKGKE--VNEFKNMVVELMTVAGYFNIGDFIPQIAWMDLQGIEKGMKKLHK 250

Query: 268 KLDSIFDKMIDERLRKADEEENRDKNDFLQFLLKVKDESDSKTPLTMVQLKALLM 313
           K D +  KM +E   +A   E + K DFL F++  +D S+ +  L++  +KALL+
Sbjct: 251 KFDDLLTKMFEE--HEATSNERKGKPDFLDFIMANRDNSEGER-LSITNIKALLL 299

[Close]

BLAST of Bhi12G000369 vs. ExPASy Swiss-Prot
Match: Q8VWZ7 (Geraniol 8-hydroxylase OS=Catharanthus roseus OX=4058 GN=CYP76B6 PE=1 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 2.8e-36
Identity = 99/304 (32.57%), Postives = 156/304 (51.32%), Query Frame = 0


Query: 23  LFTLLAAFLLIIFYVKLSRRRLQLPPGPHGVPLLGNLPFLDPELHTYFTKLGKKYGSIVK 82
           + TLL A  L   +  LSRR   LPPGP  +P +G+L  L  + H    KL KK+G I+ 
Sbjct: 7   ILTLLFALTLYEAFSYLSRRTKNLPPGPSPLPFIGSLHLLGDQPHKSLAKLSKKHGPIMS 66

Query: 83  LQLGRKICIIVNSPSVAHEVLKVHDITFANRDVPQAGRVATYGGFDITWTPYGPEWRMLR 142
           L+LG+   I+++S ++A EVL+  D+ F++R VP A        F + W P    WR LR
Sbjct: 67  LKLGQITTIVISSSTMAKEVLQKQDLAFSSRSVPNALHAHNQFKFSVVWLPVASRWRSLR 126

Query: 143 KVCAIKMLSNASLDTVYELRRREVRNTVA--QLYPRAGSVVNVGEQGFLTVFNVITSMLW 202
           KV    + S   LD    LR R+V+  +A  +   ++G  V+VG   F T  N+++++++
Sbjct: 127 KVLNSNIFSGNRLDANQHLRTRKVQELIAYCRKNSQSGEAVDVGRAAFRTSLNLLSNLIF 186

Query: 203 GGSTLEGNQKEAVAAEFRKMVSDMTELVAKPNISDFFPCLAGLDLQGIEKQMLKLVTKLD 262
                +       A EF+ +V ++     KPN+ DFFP L  +D QGI  +M     ++ 
Sbjct: 187 SKDLTDPYSDS--AKEFKDLVWNIMVEAGKPNLVDFFPLLEKVDPQGIRHRMTIHFGEVL 246

Query: 263 SIFDKMIDERLRKADEEENRDKNDFLQFLLKVKDESDSKTPLTMVQLKAL-LMVTTSDFQ 322
            +F  +++ERL +       +KND L  LL    ES  +   T ++   L L V  +D  
Sbjct: 247 KLFGGLVNERLEQ--RRSKGEKNDVLDVLLTTSQESPEEIDRTHIERMCLDLFVAGTDTT 306

Query: 323 SISI 324
           S ++
Sbjct: 307 SSTL 306

[Close]

BLAST of Bhi12G000369 vs. ExPASy Swiss-Prot
Match: P48419 (Flavonoid 3',5'-hydroxylase 2 OS=Petunia hybrida OX=4102 GN=CYP75A3 PE=2 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 1.1e-35
Identity = 99/293 (33.79%), Postives = 151/293 (51.54%), Query Frame = 0


Query: 23  LFTLLAAFLLIIFYVKLSRRRLQLPPGPHGVPLLGNLPFLDPELHTYFTKLGKKYGSIVK 82
           +F     F+  +  +   RR   LPPGP G P++G LP L    H    K+ KKYG+I+ 
Sbjct: 13  IFLTTHIFISTLLSITNGRR---LPPGPRGWPVIGALPLLGAMPHVSLAKMAKKYGAIMY 72

Query: 83  LQLGRKICIIVNSPSVAHEVLKVHDITFANRDVPQAGRV-ATYGGFDITWTPYGPEWRML 142
           L++G    ++ ++P  A   LK  D+ F+NR  P AG     YG  D+ +  YGP W++L
Sbjct: 73  LKVGTCGMVVASTPDAAKAFLKTLDLNFSNRP-PNAGATHLAYGAQDMVFAHYGPRWKLL 132

Query: 143 RKVCAIKMLSNASLDTVYELRRREVRNTVAQLY--PRAGSVVNVGEQGFLTVFNVITSML 202
           RK+  + ML   +L+    +R  E+ + +  ++   R G  V V E     + N+I  ++
Sbjct: 133 RKLSNLHMLGGKALENWANVRANELGHMLKSMFDMSREGERVVVAEMLTFAMANMIGQVI 192

Query: 203 WGGSTLEGNQKEAVAAEFRKMVSDMTELVAKPNISDFFPCLAGLDLQGIEKQMLKLVTKL 262
                     K     EF+ MV ++       NI DF PCLA +DLQGIEK M +L  K 
Sbjct: 193 LSKRVFV--NKGVEVNEFKDMVVELMTTAGYFNIGDFIPCLAWMDLQGIEKGMKRLHKKF 252

Query: 263 DSIFDKMIDERLRKADEEENRDKNDFLQFLLKVKDESDSKTPLTMVQLKALLM 313
           D++  KM DE   KA   E + K DFL  +++ +D S+ +  L+   +KALL+
Sbjct: 253 DALLTKMFDE--HKATSYERKGKPDFLDCVMENRDNSEGER-LSTTNIKALLL 296

[Close]

BLAST of Bhi12G000369 vs. ExPASy Swiss-Prot
Match: A0A1D8QMD2 (Carnosic acid synthase OS=Rosmarinus officinalis OX=39367 GN=CYP76AK7 PE=1 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 1.1e-35
Identity = 94/298 (31.54%), Postives = 163/298 (54.70%), Query Frame = 0


Query: 22  FLFTLLAAFLLIIF-YVKLSRRRL----QLPPGPHGVPLLGNLPFLDPELHTYFTKLGKK 81
           F    LAA+ +++F     SRRR     +LPPGP  +P++GN+  L  + H  F +L K 
Sbjct: 7   FSLAFLAAWFIVVFPRWSDSRRRRGPGGELPPGPPPLPIVGNILQLRGDPHKSFAQLAKT 66

Query: 82  YGSIVKLQLGRKICIIVNSPSVAHEVLKVHDITFANRDVPQAGRVATYGGFDITWTPYGP 141
           YG ++ L+LG +  ++V+SP +A E+L+ H   F+NR +P A  +  +G  +++W     
Sbjct: 67  YGPLMSLRLGTQFAVVVSSPEMATEILQKHGHAFSNRSIPDA--INIHGHNEVSWNTMPA 126

Query: 142 E---WRMLRKVCAIKMLSNASLDTVYELRRREVRNTVAQL--YPRAGSVVNVGEQGFLTV 201
           +   W+ +R+V   K+ S+ +L      R+  +R     +  +   G V+NVGE  F T+
Sbjct: 127 DSTGWKRIRRVGREKLFSHQALQQTEGQRQERLRKLADHVRGFSEQGRVMNVGEATFTTM 186

Query: 202 FNVITSMLWGGSTLEGNQKEAVA-AEFRKMVSDMTELVAKPNISDFFPCLAGLDLQGIEK 261
            +++ S L+     +    +++A  EF++ V+  T  +  PNISDFFP  A LD QGI +
Sbjct: 187 TDLVFSTLFSIDLTDYGATDSIANKEFKEHVNAFTRYIGVPNISDFFPIFAPLDPQGIRR 246

Query: 262 QMLKLVTKLDSIFDKMIDERLRKADEEENRDKNDFLQFLLKVKDESDSKTPLTMVQLK 309
           ++   +  L +    MI+ERLR+      + KNDFL  LL + +E +    L++ +++
Sbjct: 247 KIGHHLGSLLAFVQSMIEERLRERKASTYQKKNDFLDTLLDISEEGNGYDDLSIKEIR 302

[Close]

BLAST of Bhi12G000369 vs. NCBI nr
Match: XP_038907013.1 (flavonoid 3'-monooxygenase CYP75B137-like [Benincasa hispida])

HSP 1 Score: 619.8 bits (1597), Expect = 1.4e-173
Identity = 312/312 (100.00%), Postives = 312/312 (100.00%), Query Frame = 0


Query: 1   MELTFNFLLTLNFFNPNHQTCFLFTLLAAFLLIIFYVKLSRRRLQLPPGPHGVPLLGNLP 60
           MELTFNFLLTLNFFNPNHQTCFLFTLLAAFLLIIFYVKLSRRRLQLPPGPHGVPLLGNLP
Sbjct: 1   MELTFNFLLTLNFFNPNHQTCFLFTLLAAFLLIIFYVKLSRRRLQLPPGPHGVPLLGNLP 60

Query: 61  FLDPELHTYFTKLGKKYGSIVKLQLGRKICIIVNSPSVAHEVLKVHDITFANRDVPQAGR 120
           FLDPELHTYFTKLGKKYGSIVKLQLGRKICIIVNSPSVAHEVLKVHDITFANRDVPQAGR
Sbjct: 61  FLDPELHTYFTKLGKKYGSIVKLQLGRKICIIVNSPSVAHEVLKVHDITFANRDVPQAGR 120

Query: 121 VATYGGFDITWTPYGPEWRMLRKVCAIKMLSNASLDTVYELRRREVRNTVAQLYPRAGSV 180
           VATYGGFDITWTPYGPEWRMLRKVCAIKMLSNASLDTVYELRRREVRNTVAQLYPRAGSV
Sbjct: 121 VATYGGFDITWTPYGPEWRMLRKVCAIKMLSNASLDTVYELRRREVRNTVAQLYPRAGSV 180

Query: 181 VNVGEQGFLTVFNVITSMLWGGSTLEGNQKEAVAAEFRKMVSDMTELVAKPNISDFFPCL 240
           VNVGEQGFLTVFNVITSMLWGGSTLEGNQKEAVAAEFRKMVSDMTELVAKPNISDFFPCL
Sbjct: 181 VNVGEQGFLTVFNVITSMLWGGSTLEGNQKEAVAAEFRKMVSDMTELVAKPNISDFFPCL 240

Query: 241 AGLDLQGIEKQMLKLVTKLDSIFDKMIDERLRKADEEENRDKNDFLQFLLKVKDESDSKT 300
           AGLDLQGIEKQMLKLVTKLDSIFDKMIDERLRKADEEENRDKNDFLQFLLKVKDESDSKT
Sbjct: 241 AGLDLQGIEKQMLKLVTKLDSIFDKMIDERLRKADEEENRDKNDFLQFLLKVKDESDSKT 300

Query: 301 PLTMVQLKALLM 313
           PLTMVQLKALLM
Sbjct: 301 PLTMVQLKALLM 312

[Close]

BLAST of Bhi12G000369 vs. NCBI nr
Match: XP_011654786.1 (flavonoid 3'-monooxygenase CYP75B137 [Cucumis sativus] >KGN50207.1 hypothetical protein Csa_000483 [Cucumis sativus])

HSP 1 Score: 466.8 bits (1200), Expect = 1.5e-127
Identity = 241/307 (78.50%), Postives = 264/307 (85.99%), Query Frame = 0


Query: 7   FLLTLNFFNPNHQTCFLFTLLAAFLLIIFYVKLSRRRLQLPPGPHGVPLLGNLPFLDPEL 66
           FL TLN +NPN+QT FL  L A FLLI  YVKLS+ RLQLPPGP GVPLLGNLPFLDP L
Sbjct: 3   FLQTLNSYNPNNQTGFLLALFAGFLLIFLYVKLSQPRLQLPPGPRGVPLLGNLPFLDPNL 62

Query: 67  HTYFTKLGKKYGSIVKLQLGRKICIIVNSPSVAHEVLKVHDITFANRDVPQAGRVATYGG 126
           HTYF +LG+KYGSIVKLQLG K+ IIVNSPSVA E+LK HDITFANRDVPQAGRVATYGG
Sbjct: 63  HTYFMELGQKYGSIVKLQLGGKVGIIVNSPSVAREILKDHDITFANRDVPQAGRVATYGG 122

Query: 127 FDITWTPYGPEWRMLRKVCAIKMLSNASLDTVYELRRREVRNTVAQLYPRAGSVVNVGEQ 186
           FDITWTPYGPEWRMLRKVC IK+L NASLD VYELRR EVR TVAQLY RA S V +GEQ
Sbjct: 123 FDITWTPYGPEWRMLRKVCTIKLLGNASLDMVYELRRSEVRKTVAQLYQRAESTVKIGEQ 182

Query: 187 GFLTVFNVITSMLWGGSTLEGNQKEAVAAEFRKMVSDMTELVAKPNISDFFPCLAGLDLQ 246
            F TVFNVITSMLWGG T+EG +K AVA  FR+MVS MTEL  KPNISDFFP LA LD+Q
Sbjct: 183 VFFTVFNVITSMLWGG-TMEGEEKAAVAVVFREMVSAMTELAGKPNISDFFPSLACLDVQ 242

Query: 247 GIEKQMLKLVTKLDSIFDKMIDERLRKADEEE-NRDKNDFLQFLLKVKDESDSKTPLTMV 306
           GIEK+MLKL+ KLD+IF+K+IDERLR  + EE +R+KNDFLQFLLKVKDESDS+TPLT+V
Sbjct: 243 GIEKKMLKLLPKLDTIFEKLIDERLRMTNNEEGSRNKNDFLQFLLKVKDESDSQTPLTVV 302

Query: 307 QLKALLM 313
           QLKALLM
Sbjct: 303 QLKALLM 308

[Close]

BLAST of Bhi12G000369 vs. NCBI nr
Match: KAG6606160.1 (Geraniol 8-hydroxylase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 454.1 bits (1167), Expect = 1.0e-123
Identity = 227/318 (71.38%), Postives = 270/318 (84.91%), Query Frame = 0


Query: 1   MELTFNFLLTLNFFNP---NHQTCFLFTLLAAFLLIIFYVKLSRRRLQLPPGPHGVPLLG 60
           ME TFNFL +LN   P    H   F+FTLLA FL+++ + +  RRRLQLPPGP GVPLLG
Sbjct: 1   MEPTFNFLQSLNSLFPWHSGHNYPFIFTLLAGFLVLLSF-RSRRRRLQLPPGPPGVPLLG 60

Query: 61  NLPFLDPELHTYFTKLGKKYGSIVKLQLGRKICIIVNSPSVAHEVLKVHDITFANRDVPQ 120
           NLPFLDPELHTYFT+L +KYG IVKLQLG KI II+NSPSVA EVLK HDITFAN DVP+
Sbjct: 61  NLPFLDPELHTYFTELARKYGPIVKLQLGSKIGIIINSPSVAREVLKDHDITFANHDVPR 120

Query: 121 AGRVATYGGFDITWTPYGPEWRMLRKVCAIKMLSNASLDTVYELRRREVRNTVAQLYPRA 180
           A R ATYGG+DI W+PYGPEWRMLRKVC +KMLSNASLD+VYELRRREVRNTVA LY RA
Sbjct: 121 AARAATYGGYDIVWSPYGPEWRMLRKVCTMKMLSNASLDSVYELRRREVRNTVAHLYRRA 180

Query: 181 GSVVNVGEQGFLTVFNVITSMLWGGSTLEGNQKEAVAAEFRKMVSDMTELVAKPNISDFF 240
           GS VN+GEQGFLTVFN++TSMLWGGS ++G++++ +AAEFR+++SD+T L+ KPN+SDFF
Sbjct: 181 GSAVNIGEQGFLTVFNIVTSMLWGGS-VDGDERDGIAAEFRELISDITLLLGKPNVSDFF 240

Query: 241 PCLAGLDLQGIEKQMLKLVTKLDSIFDKMIDERLRKADEEENRDKNDFLQFLLKVKDESD 300
           P LA  DLQGIEKQML L  KLD+IF+KMI++RLR AD +E ++KNDFLQFLL++KDE D
Sbjct: 241 PSLARFDLQGIEKQMLNLAPKLDNIFEKMINQRLRMADADEKQNKNDFLQFLLQIKDEGD 300

Query: 301 SKTPLTMVQLKALLMVTT 316
           SKTPLTMVQ+K +LM+ T
Sbjct: 301 SKTPLTMVQIKGVLMMKT 316

[Close]

BLAST of Bhi12G000369 vs. NCBI nr
Match: XP_004147582.1 (geraniol 8-hydroxylase [Cucumis sativus] >KGN50208.1 hypothetical protein Csa_000392 [Cucumis sativus])

HSP 1 Score: 453.0 bits (1164), Expect = 2.2e-123
Identity = 229/312 (73.40%), Postives = 263/312 (84.29%), Query Frame = 0


Query: 1   MELTFNFLLTLNFFNPNHQTCFLFTLLAAFLLIIFYVKLSRRRLQLPPGPHGVPLLGNLP 60
           M LT NFL +L+FF+PN++T FL TL A FLL++ YVK SRRR+ LPPGP GVPLLGNLP
Sbjct: 1   MALTSNFLHSLDFFDPNNKTHFLLTLFAGFLLLL-YVKFSRRRIHLPPGPPGVPLLGNLP 60

Query: 61  FLDPELHTYFTKLGKKYGSIVKLQLGRKICIIVNSPSVAHEVLKVHDITFANRDVPQAGR 120
           FLDPELH+YFT+LG+KYG IV+LQLG KI IIVNS S+A EVLK HDITFAN DV +AGR
Sbjct: 61  FLDPELHSYFTELGRKYGPIVRLQLGGKIGIIVNSSSLAREVLKDHDITFANHDVHEAGR 120

Query: 121 VATYGGFDITWTPYGPEWRMLRKVCAIKMLSNASLDTVYELRRREVRNTVAQLYPRAGSV 180
            ATYGG DI+W PYGPEWRMLRKVC IKMLSNASLD+VYELRRREVRNTVA LY RAGS 
Sbjct: 121 AATYGGSDISWAPYGPEWRMLRKVCTIKMLSNASLDSVYELRRREVRNTVAHLYRRAGSA 180

Query: 181 VNVGEQGFLTVFNVITSMLWGGSTLEGNQKEAVAAEFRKMVSDMTELVAKPNISDFFPCL 240
           VNVGEQGFLT+FNV+TSMLWGGS      ++ +AAEFR+ +S++T+L+ +PN+SDFFP L
Sbjct: 181 VNVGEQGFLTIFNVVTSMLWGGSVESDQSRDNIAAEFRETISEITKLLGQPNVSDFFPSL 240

Query: 241 AGLDLQGIEKQMLKLVTKLDSIFDKMIDERLRKADEEENRDKNDFLQFLLKVKDESDSKT 300
           A  DLQGIEKQM+K V KLD+IF+KMIDER+R  D  E+  KNDFLQFLL VKDE DSKT
Sbjct: 241 ARFDLQGIEKQMVKHVLKLDTIFEKMIDERVRMKDVNESVKKNDFLQFLLTVKDEGDSKT 300

Query: 301 PLTMVQLKALLM 313
           PLTMVQLK LLM
Sbjct: 301 PLTMVQLKGLLM 311

[Close]

BLAST of Bhi12G000369 vs. NCBI nr
Match: XP_022958074.1 (geraniol 8-hydroxylase-like [Cucurbita moschata])

HSP 1 Score: 451.8 bits (1161), Expect = 5.0e-123
Identity = 231/331 (69.79%), Postives = 275/331 (83.08%), Query Frame = 0


Query: 1   MELTFNFLLTLNFFNP---NHQTCFLFTLLAAFLLIIFYVKLSRRRLQLPPGPHGVPLLG 60
           ME TFNFL +LN   P    H   F+FTLLA FL+++ + +  RRRLQLPPGP GVPLLG
Sbjct: 1   MEPTFNFLQSLNSLFPWHSGHNYPFIFTLLAGFLVLLSF-RSRRRRLQLPPGPPGVPLLG 60

Query: 61  NLPFLDPELHTYFTKLGKKYGSIVKLQLGRKICIIVNSPSVAHEVLKVHDITFANRDVPQ 120
           NLPFLDPELHTYFT+L +KYG IVKLQLG KI II+NSPSVA EVLK HDITFAN DVP+
Sbjct: 61  NLPFLDPELHTYFTELARKYGPIVKLQLGSKIGIIINSPSVAREVLKDHDITFANHDVPR 120

Query: 121 AGRVATYGGFDITWTPYGPEWRMLRKVCAIKMLSNASLDTVYELRRREVRNTVAQLYPRA 180
           A R ATYGG+DI W+PYGPEWRM+RKVC IKMLSNASLD+VYELRRREVRNTVA LY RA
Sbjct: 121 AARAATYGGYDIVWSPYGPEWRMMRKVCTIKMLSNASLDSVYELRRREVRNTVAHLYRRA 180

Query: 181 GSVVNVGEQGFLTVFNVITSMLWGGSTLEGNQKEAVAAEFRKMVSDMTELVAKPNISDFF 240
           GS VN+GEQGFLTVFN++TSMLWGGS ++G++++ +AAEFR+++SD+T L+ KPN+SDFF
Sbjct: 181 GSAVNIGEQGFLTVFNIVTSMLWGGS-VDGDERDGIAAEFRELISDITLLLGKPNVSDFF 240

Query: 241 PCLAGLDLQGIEKQMLKLVTKLDSIFDKMIDERLRKADEEENRDKNDFLQFLLKVKDESD 300
           P LA  DLQGIEKQML L  KLD+IF+KMI++RLR AD +E ++KNDFLQFLL++KDE D
Sbjct: 241 PSLARFDLQGIEKQMLNLAPKLDNIFEKMINQRLRMADADEKQNKNDFLQFLLQIKDEGD 300

Query: 301 SKTPLTMVQLKALLM---VTTSDFQSISIMF 326
           SKTPLTMVQ+K +LM   V  +D  S +I F
Sbjct: 301 SKTPLTMVQIKGVLMDMVVGGTDTSSNTIEF 329

[Close]

BLAST of Bhi12G000369 vs. ExPASy TrEMBL
Match: A0A0A0KP59 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G159610 PE=3 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 7.3e-128
Identity = 241/307 (78.50%), Postives = 264/307 (85.99%), Query Frame = 0


Query: 7   FLLTLNFFNPNHQTCFLFTLLAAFLLIIFYVKLSRRRLQLPPGPHGVPLLGNLPFLDPEL 66
           FL TLN +NPN+QT FL  L A FLLI  YVKLS+ RLQLPPGP GVPLLGNLPFLDP L
Sbjct: 3   FLQTLNSYNPNNQTGFLLALFAGFLLIFLYVKLSQPRLQLPPGPRGVPLLGNLPFLDPNL 62

Query: 67  HTYFTKLGKKYGSIVKLQLGRKICIIVNSPSVAHEVLKVHDITFANRDVPQAGRVATYGG 126
           HTYF +LG+KYGSIVKLQLG K+ IIVNSPSVA E+LK HDITFANRDVPQAGRVATYGG
Sbjct: 63  HTYFMELGQKYGSIVKLQLGGKVGIIVNSPSVAREILKDHDITFANRDVPQAGRVATYGG 122

Query: 127 FDITWTPYGPEWRMLRKVCAIKMLSNASLDTVYELRRREVRNTVAQLYPRAGSVVNVGEQ 186
           FDITWTPYGPEWRMLRKVC IK+L NASLD VYELRR EVR TVAQLY RA S V +GEQ
Sbjct: 123 FDITWTPYGPEWRMLRKVCTIKLLGNASLDMVYELRRSEVRKTVAQLYQRAESTVKIGEQ 182

Query: 187 GFLTVFNVITSMLWGGSTLEGNQKEAVAAEFRKMVSDMTELVAKPNISDFFPCLAGLDLQ 246
            F TVFNVITSMLWGG T+EG +K AVA  FR+MVS MTEL  KPNISDFFP LA LD+Q
Sbjct: 183 VFFTVFNVITSMLWGG-TMEGEEKAAVAVVFREMVSAMTELAGKPNISDFFPSLACLDVQ 242

Query: 247 GIEKQMLKLVTKLDSIFDKMIDERLRKADEEE-NRDKNDFLQFLLKVKDESDSKTPLTMV 306
           GIEK+MLKL+ KLD+IF+K+IDERLR  + EE +R+KNDFLQFLLKVKDESDS+TPLT+V
Sbjct: 243 GIEKKMLKLLPKLDTIFEKLIDERLRMTNNEEGSRNKNDFLQFLLKVKDESDSQTPLTVV 302

Query: 307 QLKALLM 313
           QLKALLM
Sbjct: 303 QLKALLM 308

[Close]

BLAST of Bhi12G000369 vs. ExPASy TrEMBL
Match: A0A0A0KKS3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G159620 PE=3 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 1.1e-123
Identity = 229/312 (73.40%), Postives = 263/312 (84.29%), Query Frame = 0


Query: 1   MELTFNFLLTLNFFNPNHQTCFLFTLLAAFLLIIFYVKLSRRRLQLPPGPHGVPLLGNLP 60
           M LT NFL +L+FF+PN++T FL TL A FLL++ YVK SRRR+ LPPGP GVPLLGNLP
Sbjct: 1   MALTSNFLHSLDFFDPNNKTHFLLTLFAGFLLLL-YVKFSRRRIHLPPGPPGVPLLGNLP 60

Query: 61  FLDPELHTYFTKLGKKYGSIVKLQLGRKICIIVNSPSVAHEVLKVHDITFANRDVPQAGR 120
           FLDPELH+YFT+LG+KYG IV+LQLG KI IIVNS S+A EVLK HDITFAN DV +AGR
Sbjct: 61  FLDPELHSYFTELGRKYGPIVRLQLGGKIGIIVNSSSLAREVLKDHDITFANHDVHEAGR 120

Query: 121 VATYGGFDITWTPYGPEWRMLRKVCAIKMLSNASLDTVYELRRREVRNTVAQLYPRAGSV 180
            ATYGG DI+W PYGPEWRMLRKVC IKMLSNASLD+VYELRRREVRNTVA LY RAGS 
Sbjct: 121 AATYGGSDISWAPYGPEWRMLRKVCTIKMLSNASLDSVYELRRREVRNTVAHLYRRAGSA 180

Query: 181 VNVGEQGFLTVFNVITSMLWGGSTLEGNQKEAVAAEFRKMVSDMTELVAKPNISDFFPCL 240
           VNVGEQGFLT+FNV+TSMLWGGS      ++ +AAEFR+ +S++T+L+ +PN+SDFFP L
Sbjct: 181 VNVGEQGFLTIFNVVTSMLWGGSVESDQSRDNIAAEFRETISEITKLLGQPNVSDFFPSL 240

Query: 241 AGLDLQGIEKQMLKLVTKLDSIFDKMIDERLRKADEEENRDKNDFLQFLLKVKDESDSKT 300
           A  DLQGIEKQM+K V KLD+IF+KMIDER+R  D  E+  KNDFLQFLL VKDE DSKT
Sbjct: 241 ARFDLQGIEKQMVKHVLKLDTIFEKMIDERVRMKDVNESVKKNDFLQFLLTVKDEGDSKT 300

Query: 301 PLTMVQLKALLM 313
           PLTMVQLK LLM
Sbjct: 301 PLTMVQLKGLLM 311

[Close]

BLAST of Bhi12G000369 vs. ExPASy TrEMBL
Match: A0A6J1H412 (geraniol 8-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111459411 PE=3 SV=1)

HSP 1 Score: 451.8 bits (1161), Expect = 2.4e-123
Identity = 231/331 (69.79%), Postives = 275/331 (83.08%), Query Frame = 0


Query: 1   MELTFNFLLTLNFFNP---NHQTCFLFTLLAAFLLIIFYVKLSRRRLQLPPGPHGVPLLG 60
           ME TFNFL +LN   P    H   F+FTLLA FL+++ + +  RRRLQLPPGP GVPLLG
Sbjct: 1   MEPTFNFLQSLNSLFPWHSGHNYPFIFTLLAGFLVLLSF-RSRRRRLQLPPGPPGVPLLG 60

Query: 61  NLPFLDPELHTYFTKLGKKYGSIVKLQLGRKICIIVNSPSVAHEVLKVHDITFANRDVPQ 120
           NLPFLDPELHTYFT+L +KYG IVKLQLG KI II+NSPSVA EVLK HDITFAN DVP+
Sbjct: 61  NLPFLDPELHTYFTELARKYGPIVKLQLGSKIGIIINSPSVAREVLKDHDITFANHDVPR 120

Query: 121 AGRVATYGGFDITWTPYGPEWRMLRKVCAIKMLSNASLDTVYELRRREVRNTVAQLYPRA 180
           A R ATYGG+DI W+PYGPEWRM+RKVC IKMLSNASLD+VYELRRREVRNTVA LY RA
Sbjct: 121 AARAATYGGYDIVWSPYGPEWRMMRKVCTIKMLSNASLDSVYELRRREVRNTVAHLYRRA 180

Query: 181 GSVVNVGEQGFLTVFNVITSMLWGGSTLEGNQKEAVAAEFRKMVSDMTELVAKPNISDFF 240
           GS VN+GEQGFLTVFN++TSMLWGGS ++G++++ +AAEFR+++SD+T L+ KPN+SDFF
Sbjct: 181 GSAVNIGEQGFLTVFNIVTSMLWGGS-VDGDERDGIAAEFRELISDITLLLGKPNVSDFF 240

Query: 241 PCLAGLDLQGIEKQMLKLVTKLDSIFDKMIDERLRKADEEENRDKNDFLQFLLKVKDESD 300
           P LA  DLQGIEKQML L  KLD+IF+KMI++RLR AD +E ++KNDFLQFLL++KDE D
Sbjct: 241 PSLARFDLQGIEKQMLNLAPKLDNIFEKMINQRLRMADADEKQNKNDFLQFLLQIKDEGD 300

Query: 301 SKTPLTMVQLKALLM---VTTSDFQSISIMF 326
           SKTPLTMVQ+K +LM   V  +D  S +I F
Sbjct: 301 SKTPLTMVQIKGVLMDMVVGGTDTSSNTIEF 329

[Close]

BLAST of Bhi12G000369 vs. ExPASy TrEMBL
Match: A0A5A7TE08 (Geraniol 8-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold558G00060 PE=3 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 6.0e-122
Identity = 233/316 (73.73%), Postives = 263/316 (83.23%), Query Frame = 0


Query: 1   MELTFNFLLT----LNFFNPNHQTCFLFTLLAAFLLIIFYVKLSRRRLQLPPGPHGVPLL 60
           MELT NFL T    LNF+    +T FLFTL A FLL++ YVKLS+RR+ LPPGP GVPLL
Sbjct: 1   MELTSNFLQTFIHSLNFY----KTHFLFTLFAGFLLLL-YVKLSQRRIHLPPGPTGVPLL 60

Query: 61  GNLPFLDPELHTYFTKLGKKYGSIVKLQLGRKICIIVNSPSVAHEVLKVHDITFANRDVP 120
           GNLPFLDPELH+YFT+LG+KYG IV+L+LG KI IIVNS SVA EVLK HDITFANRDVP
Sbjct: 61  GNLPFLDPELHSYFTELGRKYGPIVRLRLGGKIGIIVNSSSVAREVLKDHDITFANRDVP 120

Query: 121 QAGRVATYGGFDITWTPYGPEWRMLRKVCAIKMLSNASLDTVYELRRREVRNTVAQLYPR 180
           QAGR ATYGG+DI+W PYGPEWRMLRKVC +KMLSNASLD+VYELRRREVR TVA LY R
Sbjct: 121 QAGRAATYGGYDISWAPYGPEWRMLRKVCTVKMLSNASLDSVYELRRREVRKTVAHLYRR 180

Query: 181 AGSVVNVGEQGFLTVFNVITSMLWGGSTLEGNQKEAVAAEFRKMVSDMTELVAKPNISDF 240
           AGS VNVGEQGFLT+FNV+TSMLWGGS      ++ +AA+FR+ VSD+T+L+  PN+SDF
Sbjct: 181 AGSAVNVGEQGFLTIFNVVTSMLWGGSVENDQSRDDIAADFRETVSDVTKLLGLPNVSDF 240

Query: 241 FPCLAGLDLQGIEKQMLKLVTKLDSIFDKMIDERLRKADEEENRDKNDFLQFLLKVKDES 300
           FP LA  DLQGIEKQM K V KLD+IF+KMIDERLR  D  E+  K+DFLQFLLKVKDE 
Sbjct: 241 FPSLARFDLQGIEKQMGKHVQKLDAIFEKMIDERLRMKDVNESAKKDDFLQFLLKVKDEG 300

Query: 301 DSKTPLTMVQLKALLM 313
           DSKTPLTMVQLK LLM
Sbjct: 301 DSKTPLTMVQLKGLLM 311

[Close]

BLAST of Bhi12G000369 vs. ExPASy TrEMBL
Match: A0A1S3ATU1 (geraniol 8-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103482650 PE=3 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 6.0e-122
Identity = 233/316 (73.73%), Postives = 263/316 (83.23%), Query Frame = 0


Query: 1   MELTFNFLLT----LNFFNPNHQTCFLFTLLAAFLLIIFYVKLSRRRLQLPPGPHGVPLL 60
           MELT NFL T    LNF+    +T FLFTL A FLL++ YVKLS+RR+ LPPGP GVPLL
Sbjct: 1   MELTSNFLQTFIHSLNFY----KTHFLFTLFAGFLLLL-YVKLSQRRIHLPPGPTGVPLL 60

Query: 61  GNLPFLDPELHTYFTKLGKKYGSIVKLQLGRKICIIVNSPSVAHEVLKVHDITFANRDVP 120
           GNLPFLDPELH+YFT+LG+KYG IV+L+LG KI IIVNS SVA EVLK HDITFANRDVP
Sbjct: 61  GNLPFLDPELHSYFTELGRKYGPIVRLRLGGKIGIIVNSSSVAREVLKDHDITFANRDVP 120

Query: 121 QAGRVATYGGFDITWTPYGPEWRMLRKVCAIKMLSNASLDTVYELRRREVRNTVAQLYPR 180
           QAGR ATYGG+DI+W PYGPEWRMLRKVC +KMLSNASLD+VYELRRREVR TVA LY R
Sbjct: 121 QAGRAATYGGYDISWAPYGPEWRMLRKVCTVKMLSNASLDSVYELRRREVRKTVAHLYRR 180

Query: 181 AGSVVNVGEQGFLTVFNVITSMLWGGSTLEGNQKEAVAAEFRKMVSDMTELVAKPNISDF 240
           AGS VNVGEQGFLT+FNV+TSMLWGGS      ++ +AA+FR+ VSD+T+L+  PN+SDF
Sbjct: 181 AGSAVNVGEQGFLTIFNVVTSMLWGGSVENDQSRDDIAADFRETVSDVTKLLGLPNVSDF 240

Query: 241 FPCLAGLDLQGIEKQMLKLVTKLDSIFDKMIDERLRKADEEENRDKNDFLQFLLKVKDES 300
           FP LA  DLQGIEKQM K V KLD+IF+KMIDERLR  D  E+  K+DFLQFLLKVKDE 
Sbjct: 241 FPSLARFDLQGIEKQMGKHVQKLDAIFEKMIDERLRMKDVNESAKKDDFLQFLLKVKDEG 300

Query: 301 DSKTPLTMVQLKALLM 313
           DSKTPLTMVQLK LLM
Sbjct: 301 DSKTPLTMVQLKGLLM 311

The following BLAST results are available for this feature:

BLAST of Bhi12G000369 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G12300.1	5.6e-80	49.67	cytochrome P450, family 706, subfamily A, polypeptide 4	[more]
AT4G12320.1	3.6e-79	48.01	cytochrome P450, family 706, subfamily A, polypeptide 6	[more]
AT5G44620.1	1.1e-78	53.05	cytochrome P450, family 706, subfamily A, polypeptide 3	[more]
AT4G12310.1	1.4e-78	51.03	cytochrome P450, family 706, subfamily A, polypeptide 5	[more]
AT4G12330.1	1.3e-73	47.75	cytochrome P450, family 706, subfamily A, polypeptide 7	[more]

BLAST of Bhi12G000369 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A4D6Q415	9.4e-40	32.99	Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN...	[more]
P37120	4.4e-37	33.22	Flavonoid 3',5'-hydroxylase OS=Solanum melongena OX=4111 GN=CYP75A2 PE=2 SV=1	[more]
Q8VWZ7	2.8e-36	32.57	Geraniol 8-hydroxylase OS=Catharanthus roseus OX=4058 GN=CYP76B6 PE=1 SV=1	[more]
P48419	1.1e-35	33.79	Flavonoid 3',5'-hydroxylase 2 OS=Petunia hybrida OX=4102 GN=CYP75A3 PE=2 SV=1	[more]
A0A1D8QMD2	1.1e-35	31.54	Carnosic acid synthase OS=Rosmarinus officinalis OX=39367 GN=CYP76AK7 PE=1 SV=1	[more]

BLAST of Bhi12G000369 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038907013.1	1.4e-173	100.00	flavonoid 3'-monooxygenase CYP75B137-like [Benincasa hispida]	[more]
XP_011654786.1	1.5e-127	78.50	flavonoid 3'-monooxygenase CYP75B137 [Cucumis sativus] >KGN50207.1 hypothetical ...	[more]
KAG6606160.1	1.0e-123	71.38	Geraniol 8-hydroxylase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_004147582.1	2.2e-123	73.40	geraniol 8-hydroxylase [Cucumis sativus] >KGN50208.1 hypothetical protein Csa_00...	[more]
XP_022958074.1	5.0e-123	69.79	geraniol 8-hydroxylase-like [Cucurbita moschata]	[more]

BLAST of Bhi12G000369 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KP59	7.3e-128	78.50	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G159610 PE=3 SV=1	[more]
A0A0A0KKS3	1.1e-123	73.40	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G159620 PE=3 SV=1	[more]
A0A6J1H412	2.4e-123	69.79	geraniol 8-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111459411 PE=3 S...	[more]
A0A5A7TE08	6.0e-122	73.73	Geraniol 8-hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf...	[more]
A0A1S3ATU1	6.0e-122	73.73	geraniol 8-hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103482650 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 47..312 e-value: 9.7E-32 score: 110.3
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 39..327 e-value: 1.8E-47 score: 164.0
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 39..313
None	No IPR available	PANTHER	PTHR47951:SF3	CYTOCHROME P450, FAMILY 706, SUBFAMILY A, POLYPEPTIDE 3-RELATED	coord: 12..312
None	No IPR available	PANTHER	PTHR47951	OS08G0547900 PROTEIN	coord: 12..312

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi12M000369	Bhi12M000369	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen