Bhi12G000337 (gene) Wax gourd (B227) v1

BLAST of Bhi12G000337 vs. TAIR 10
Match: AT1G12420.1 (ACT domain repeat 8 )

HSP 1 Score: 569.3 bits (1466), Expect = 2.7e-162
Identity = 292/443 (65.91%), Postives = 354/443 (79.91%), Query Frame = 0

Query: 4   MEWPGSLDEYSKLINRMNTPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLNLSI 63
           M   G LDEY KL+ RMNTPRVVIDN VC +AT+VKVDS+RR+GILLEAVQ+LTDLNLSI
Sbjct: 1   MAMKGYLDEYEKLVIRMNTPRVVIDNGVCSSATIVKVDSSRRNGILLEAVQILTDLNLSI 60

Query: 64  QKAYISSDGIWFMDVFHVTDLDGNKLTDEGVISYLEESLATIRCGKPAASNDLTALELTG 123
           +KAYISSDG W MDVFHVTDL+GNKL D+ V+ Y+E+S+ T+  G+    N LTALELTG
Sbjct: 61  KKAYISSDGTWNMDVFHVTDLNGNKLNDQSVLRYIEQSIETVYYGENIEVNGLTALELTG 120

Query: 124 TDRVGLLSEVFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDSQKIDTIVGR 183
           TDR+GLLSE+FAVL++L CDVV+AK+WTHNGR+AS+IY+KDC SG+PI DS +I  I GR
Sbjct: 121 TDRIGLLSEMFAVLSDLNCDVVDAKLWTHNGRVASVIYLKDCISGAPILDSHRISKIEGR 180

Query: 184 LRNVLKGDDDI-LYAKTSVAM-TVTHTERRLHQMMFADRDYERKPVLQHSEDSPVVTVQN 243
           L+NVL GD+D+   AKT V + ++ H ERRLHQ+MF DRDYER+          VVTVQN
Sbjct: 181 LKNVLNGDNDVNSAAKTCVTVDSMMHIERRLHQLMFEDRDYERRSKKHERSPMVVVTVQN 240

Query: 244 LVERGYSVVNVQCKDRMKLLFDVVCTMTDMDYVVFHGTVATTSHRAYLEFYIRHTDGTPI 303
             ERGYSVVNV C+DR KLLFDVVCT+TDM+Y VFH T+ T   +A+LEFYIRH DG+PI
Sbjct: 241 WAERGYSVVNVHCRDRTKLLFDVVCTLTDMEYAVFHATINTAEDQAHLEFYIRHKDGSPI 300

Query: 304 SSEAERQRVIQCLQASVERRTSRGVRLELRTTDRPCLLADVTRTFRENGLNVTRAEVSTS 363
           SSEAERQRVIQCL+A+VERR   GVRLELR  D+  LLA+VTRTFRENGLNVTR E+STS
Sbjct: 301 SSEAERQRVIQCLEAAVERRALEGVRLELRHPDKQGLLAEVTRTFRENGLNVTRTEISTS 360

Query: 364 ADVALNLFYVTDGHGSAVDTKTIDSVREKIGMSSLKVKELPLISQQKTEGEEQGAGVGGA 423
           +D+A N+FYVTD +G   D K I+SVREKIG+  L+VKE+P +  +K +GEEQ      A
Sbjct: 361 SDMATNIFYVTDANGDEPDFKLIESVREKIGLECLRVKEMPTMYHKKGDGEEQQQ--TKA 420

Query: 424 VLLSLGSILRRNLYNLGLIRSYS 445
           VL+SLGS++ RNL+N GLI+S S
Sbjct: 421 VLVSLGSLVWRNLFNFGLIKSCS 441

BLAST of Bhi12G000337 vs. TAIR 10
Match: AT4G22780.1 (ACT domain repeat 7 )

HSP 1 Score: 529.6 bits (1363), Expect = 2.4e-150
Identity = 280/451 (62.08%), Postives = 344/451 (76.27%), Query Frame = 0

Query: 4   MEWPGSLDEYSKLINRMNTPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLNLSI 63
           ME     +EY KL+ RMN PRVVIDN VC  +T+VK+DSAR  GILLE+VQ+LTD+NL I
Sbjct: 1   MELSDCSNEYEKLVVRMNMPRVVIDNGVCPNSTVVKIDSARSPGILLESVQLLTDMNLWI 60

Query: 64  QKAYISSDGIWFMDVFHVTDLDGNKLTDEGVISYLEESLATIRCGKPAASNDLTALELTG 123
           +KAYISSDG W MDVFHV+DL+G+KLTDE +I Y+E+S+ T    K      LTALELTG
Sbjct: 61  KKAYISSDGKWNMDVFHVSDLNGDKLTDENLIRYIEKSIETSHYCKTEGYTGLTALELTG 120

Query: 124 TDRVGLLSEVFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIK-DSQKIDTIVG 183
           TDRVGLLSEVFAVLA+L+CDVVEAK WTHNGRIAS+IYVKD NSG+PI  DS ++  + G
Sbjct: 121 TDRVGLLSEVFAVLADLECDVVEAKAWTHNGRIASMIYVKDGNSGTPIDGDSDRVQRVEG 180

Query: 184 RLRNVLKGDDDILY-AKTSVAM-TVTHTERRLHQMMFADRDYERKPVLQHSEDSPVVTVQ 243
           +LRN+LK DD      +T V+    TH ERRLHQ MF DRDYE+K  +   E SP+V+VQ
Sbjct: 181 QLRNLLKADDGYQNDTRTCVSYGGNTHMERRLHQRMFMDRDYEKKFDI---EKSPIVSVQ 240

Query: 244 NLVERGYSVVNVQCKDRMKLLFDVVCTMTDMDYVVFHGTVATTSHRAYLEFYIRHTDGTP 303
           NL +RGYSVVN+QCKDRMKLLFDVVCT+TDM Y+VFH  + T    A+LEFY+RH+DG P
Sbjct: 241 NLPKRGYSVVNLQCKDRMKLLFDVVCTLTDMAYIVFHAAIRTVGETAFLEFYVRHSDGHP 300

Query: 304 ISSEAERQRVIQCLQASVERRTSRGVRLELRTTDRPCLLADVTRTFRENGLNVTRAEVST 363
           +SSE ERQR+IQCLQA++ERRT +GVRLEL T DRP LLA+VTR  RENGLN+ RAE+ST
Sbjct: 301 VSSEPERQRLIQCLQAAIERRTVKGVRLELCTADRPGLLAEVTRILRENGLNIARAEIST 360

Query: 364 SADVALNLFYVTDGHGSAVDTKTIDSVREKIGMSSLKVKE-LPLISQQKTEGEE------ 423
              +A N+FYVTD +G+ +D + I S+REKIG+  L VKE  P+  ++  E E+      
Sbjct: 361 KDSIARNVFYVTDANGNLIDPEIIKSIREKIGIDDLSVKEPFPISCREAVEKEQHIEPQD 420

Query: 424 -QGAGVGGAVLLSLGSILRRNLYNLGLIRSY 444
            QG   GG VL+SLGS++ RNLY+LGLI+SY
Sbjct: 421 HQGRYGGGTVLVSLGSLVMRNLYHLGLIKSY 448

BLAST of Bhi12G000337 vs. TAIR 10
Match: AT1G69040.2 (ACT domain repeat 4 )

HSP 1 Score: 448.7 bits (1153), Expect = 5.3e-126
Identity = 242/456 (53.07%), Postives = 308/456 (67.54%), Query Frame = 0

Query: 2   VEMEWPGSLD-EYSKLINRMNTPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLN 61
           V M +   +D EY KLI RMN PRVVIDN  C+ AT+++VDSA  +GILLE VQ+LTDLN
Sbjct: 3   VSMSFSQDMDNEYEKLIRRMNPPRVVIDNDSCKKATVIRVDSANEYGILLEVVQILTDLN 62

Query: 62  LSIQKAYISSDGIWFMDVFHVTDLDGNKLTDEGVISYLEESLATIRCGKPA-------AS 121
           L+I KAYISSDG WFMDVF+VTD DGNK+TDE V+ Y+++SL    C   +        S
Sbjct: 63  LTITKAYISSDGGWFMDVFNVTDQDGNKVTDEVVLDYIQKSLGPEACFSTSMRSVGVIPS 122

Query: 122 NDLTALELTGTDRVGLLSEVFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIKD 181
            D T +ELTG DR GLLSE+ AVL  L+C V+ A++WTHN R A+++ V D  +G  I D
Sbjct: 123 TDSTVIELTGCDRPGLLSELSAVLTHLKCSVLNAEIWTHNTRAAAVMQVTDDLTGCGISD 182

Query: 182 SQKIDTIVGRLRNVLKGDDDILYAKTSVAMTVTHTERRLHQMMFADRDYERKPV-----L 241
            +++  I   LRNVLKG +    AKT V+    HT+RRLHQMMF DRDYE + V     +
Sbjct: 183 PERLSRIKNLLRNVLKGSNTPREAKTVVSHGEVHTDRRLHQMMFEDRDYEHRLVDDDSSI 242

Query: 242 QHSEDSPVVTVQNLVERGYSVVNVQCKDRMKLLFDVVCTMTDMDYVVFHGTVATTSHRAY 301
           Q     P V V N +++ YSVV V+CKDR KLLFD VCT+TDM YVVFHG+V T    A+
Sbjct: 243 QDERQRPDVCVDNWLDKDYSVVTVRCKDRPKLLFDTVCTLTDMQYVVFHGSVDTEGTEAF 302

Query: 302 LEFYIRHTDGTPISSEAERQRVIQCLQASVERRTSRGVRLELRTTDRPCLLADVTRTFRE 361
            E+Y+RH DG+P+ SEAE+QRVIQCL+A+++RR S G++LEL TTDR  LL++VTR FRE
Sbjct: 303 QEYYVRHIDGSPVKSEAEKQRVIQCLEAAIKRRVSEGLKLELCTTDRVGLLSNVTRIFRE 362

Query: 362 NGLNVTRAEVSTSADVALNLFYVTDGHGSAVDTKTIDSVREKIGMSSLKVKELPLISQQK 421
           N L VTRAEV T    ALN FYV+D  G ++D KTIDS+R+ IG + LKVK  P   QQ+
Sbjct: 363 NSLTVTRAEVKTKGGKALNTFYVSDASGYSIDAKTIDSIRQTIGQTILKVKNNPQEQQQR 422

Query: 422 TEGEEQGAGVGGAVLLSLGSILRRNLYNLGLIRSYS 445
            +     +       L  G    ++  N GL+RSYS
Sbjct: 423 QKSPSHESPTR---FLFGGLFKSKSFVNFGLVRSYS 455

BLAST of Bhi12G000337 vs. TAIR 10
Match: AT1G69040.1 (ACT domain repeat 4 )

HSP 1 Score: 447.6 bits (1150), Expect = 1.2e-125
Identity = 239/446 (53.59%), Postives = 304/446 (68.16%), Query Frame = 0

Query: 11  DEYSKLINRMNTPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLNLSIQKAYISS 70
           +EY KLI RMN PRVVIDN  C+ AT+++VDSA  +GILLE VQ+LTDLNL+I KAYISS
Sbjct: 9   NEYEKLIRRMNPPRVVIDNDSCKKATVIRVDSANEYGILLEVVQILTDLNLTITKAYISS 68

Query: 71  DGIWFMDVFHVTDLDGNKLTDEGVISYLEESLATIRCGKPA-------ASNDLTALELTG 130
           DG WFMDVF+VTD DGNK+TDE V+ Y+++SL    C   +        S D T +ELTG
Sbjct: 69  DGGWFMDVFNVTDQDGNKVTDEVVLDYIQKSLGPEACFSTSMRSVGVIPSTDSTVIELTG 128

Query: 131 TDRVGLLSEVFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDSQKIDTIVGR 190
            DR GLLSE+ AVL  L+C V+ A++WTHN R A+++ V D  +G  I D +++  I   
Sbjct: 129 CDRPGLLSELSAVLTHLKCSVLNAEIWTHNTRAAAVMQVTDDLTGCGISDPERLSRIKNL 188

Query: 191 LRNVLKGDDDILYAKTSVAMTVTHTERRLHQMMFADRDYERKPV-----LQHSEDSPVVT 250
           LRNVLKG +    AKT V+    HT+RRLHQMMF DRDYE + V     +Q     P V 
Sbjct: 189 LRNVLKGSNTPREAKTVVSHGEVHTDRRLHQMMFEDRDYEHRLVDDDSSIQDERQRPDVC 248

Query: 251 VQNLVERGYSVVNVQCKDRMKLLFDVVCTMTDMDYVVFHGTVATTSHRAYLEFYIRHTDG 310
           V N +++ YSVV V+CKDR KLLFD VCT+TDM YVVFHG+V T    A+ E+Y+RH DG
Sbjct: 249 VDNWLDKDYSVVTVRCKDRPKLLFDTVCTLTDMQYVVFHGSVDTEGTEAFQEYYVRHIDG 308

Query: 311 TPISSEAERQRVIQCLQASVERRTSRGVRLELRTTDRPCLLADVTRTFRENGLNVTRAEV 370
           +P+ SEAE+QRVIQCL+A+++RR S G++LEL TTDR  LL++VTR FREN L VTRAEV
Sbjct: 309 SPVKSEAEKQRVIQCLEAAIKRRVSEGLKLELCTTDRVGLLSNVTRIFRENSLTVTRAEV 368

Query: 371 STSADVALNLFYVTDGHGSAVDTKTIDSVREKIGMSSLKVKELPLISQQKTEGEEQGAGV 430
            T    ALN FYV+D  G ++D KTIDS+R+ IG + LKVK  P   QQ+ +     +  
Sbjct: 369 KTKGGKALNTFYVSDASGYSIDAKTIDSIRQTIGQTILKVKNNPQEQQQRQKSPSHESPT 428

Query: 431 GGAVLLSLGSILRRNLYNLGLIRSYS 445
                L  G    ++  N GL+RSYS
Sbjct: 429 R---FLFGGLFKSKSFVNFGLVRSYS 451

BLAST of Bhi12G000337 vs. TAIR 10
Match: AT2G03730.1 (ACT domain repeat 5 )

HSP 1 Score: 409.1 bits (1050), Expect = 4.6e-114
Identity = 232/452 (51.33%), Postives = 297/452 (65.71%), Query Frame = 0

Query: 11  DEYSKLINRMNTPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLNLSIQKAYISS 70
           DE +K I R+N PRVVIDN VC+  T++KVDSA +HGILLE VQVLT+LNL+I+KAYISS
Sbjct: 13  DEIAKFIRRVNPPRVVIDNEVCKDVTVIKVDSANKHGILLEVVQVLTELNLTIKKAYISS 72

Query: 71  DGIWFMDVFHVTDLDGNKLTDEGVISYLEESLA--TIRCGKPA--------ASNDLTALE 130
           DG WFMDVF+VTD DGNK+TDE V+ Y+ +SL      C  P+         S D T +E
Sbjct: 73  DGGWFMDVFNVTDQDGNKVTDEIVLEYIRKSLGPDESSCFSPSMRSTIGVKQSVDYTVVE 132

Query: 131 LTGTDRVGLLSEVFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDSQKIDTI 190
           LTGTDR GLLSE+ AVL +LQC+VV A++WTH  + A+++ V D  + S I D +++  I
Sbjct: 133 LTGTDRPGLLSELCAVLMDLQCNVVNAEIWTHRAKAAAVLQVTDEETCSAITDPERLSKI 192

Query: 191 VGRLRNVLKGDDD---ILYAKTSV--AMTVTHTERRLHQMMFADRDYERKPVLQHSEDS- 250
              L  VL G          KT+V  A+  THT+R+LHQ+MFADRDY+        ED  
Sbjct: 193 RKLLGYVLTGGSSGRRFREPKTTVSSALNETHTDRKLHQLMFADRDYDEWENNVDDEDKC 252

Query: 251 ----PVVTVQNLVERGYSVVNVQCKDRMKLLFDVVCTMTDMDYVVFHGTVATTSHRAYLE 310
               P V V NL +  YS+V ++CKDR KLLFD V T+TDM+YVV H ++     +AY E
Sbjct: 253 GRVIPDVDVSNLHDLDYSIVMIKCKDRPKLLFDTVFTLTDMNYVVSHASIDAEGPQAYQE 312

Query: 311 FYIRHTDGTPISSEAERQRVIQCLQASVERRTSRGVRLELRTTDRPCLLADVTRTFRENG 370
           +YIRHTDG+P+ SEAERQRVI+CL+A+++RR S G++LEL T+DR  LL+DVTR FREN 
Sbjct: 313 YYIRHTDGSPVKSEAERQRVIKCLKAAIQRRVSEGLKLELCTSDRVGLLSDVTRIFRENS 372

Query: 371 LNVTRAEVSTSADVALNLFYVTDGHGSAVDTKTIDSVREKIGMSSLKVKELPLISQQKTE 430
           L VTRAEV T  D ALN FYV D  G  VDTKTI+S+R+ IG + L+VK     ++   +
Sbjct: 373 LTVTRAEVKTKGDKALNTFYVRDASGYQVDTKTIESIRQVIGQTILQVKGGNTDAKPSPQ 432

Query: 431 GEEQGAGVGGAVLLSLGSILRRNLYNLGLIRS 443
               G           G    R+  N GLIRS
Sbjct: 433 DSPTG--------FLFGVFKSRSFVNFGLIRS 456

BLAST of Bhi12G000337 vs. ExPASy Swiss-Prot
Match: Q9LNA5 (ACT domain-containing protein ACR8 OS=Arabidopsis thaliana OX=3702 GN=ACR8 PE=2 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 3.8e-161
Identity = 292/443 (65.91%), Postives = 354/443 (79.91%), Query Frame = 0

Query: 4   MEWPGSLDEYSKLINRMNTPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLNLSI 63
           M   G LDEY KL+ RMNTPRVVIDN VC +AT+VKVDS+RR+GILLEAVQ+LTDLNLSI
Sbjct: 1   MAMKGYLDEYEKLVIRMNTPRVVIDNGVCSSATIVKVDSSRRNGILLEAVQILTDLNLSI 60

Query: 64  QKAYISSDGIWFMDVFHVTDLDGNKLTDEGVISYLEESLATIRCGKPAASNDLTALELTG 123
           +KAYISSDG W MDVFHVTDL+GNKL D+ V+ Y+E+S+ T+  G+    N LTALELTG
Sbjct: 61  KKAYISSDGTWNMDVFHVTDLNGNKLNDQSVLRYIEQSIETVYYGENIEVNGLTALELTG 120

Query: 124 TDRVGLLSEVFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDSQKIDTIVGR 183
           TDR+GLLSE+FAVL++L CDVV+AK+WTHNGR+AS+IY+KDC SG+PI DS +I  I GR
Sbjct: 121 TDRIGLLSEMFAVLSDLNCDVVDAKLWTHNGRVASVIYLKDCISGAPILDSHRISKIEGR 180

Query: 184 LRNVLKGDDDI-LYAKTSVAM-TVTHTERRLHQMMFADRDYERKPVLQHSEDSPVVTVQN 243
           L+NVL GD+D+   AKT V + ++ H ERRLHQ+MF DRDYER+          VVTVQN
Sbjct: 181 LKNVLNGDNDVNSAAKTCVTVDSMMHIERRLHQLMFEDRDYERRSKKHERSPMVVVTVQN 240

Query: 244 LVERGYSVVNVQCKDRMKLLFDVVCTMTDMDYVVFHGTVATTSHRAYLEFYIRHTDGTPI 303
             ERGYSVVNV C+DR KLLFDVVCT+TDM+Y VFH T+ T   +A+LEFYIRH DG+PI
Sbjct: 241 WAERGYSVVNVHCRDRTKLLFDVVCTLTDMEYAVFHATINTAEDQAHLEFYIRHKDGSPI 300

Query: 304 SSEAERQRVIQCLQASVERRTSRGVRLELRTTDRPCLLADVTRTFRENGLNVTRAEVSTS 363
           SSEAERQRVIQCL+A+VERR   GVRLELR  D+  LLA+VTRTFRENGLNVTR E+STS
Sbjct: 301 SSEAERQRVIQCLEAAVERRALEGVRLELRHPDKQGLLAEVTRTFRENGLNVTRTEISTS 360

Query: 364 ADVALNLFYVTDGHGSAVDTKTIDSVREKIGMSSLKVKELPLISQQKTEGEEQGAGVGGA 423
           +D+A N+FYVTD +G   D K I+SVREKIG+  L+VKE+P +  +K +GEEQ      A
Sbjct: 361 SDMATNIFYVTDANGDEPDFKLIESVREKIGLECLRVKEMPTMYHKKGDGEEQQQ--TKA 420

Query: 424 VLLSLGSILRRNLYNLGLIRSYS 445
           VL+SLGS++ RNL+N GLI+S S
Sbjct: 421 VLVSLGSLVWRNLFNFGLIKSCS 441

BLAST of Bhi12G000337 vs. ExPASy Swiss-Prot
Match: Q8LJW1 (ACT domain-containing protein ACR7 OS=Arabidopsis thaliana OX=3702 GN=ACR7 PE=2 SV=1)

HSP 1 Score: 529.6 bits (1363), Expect = 3.3e-149
Identity = 280/451 (62.08%), Postives = 344/451 (76.27%), Query Frame = 0

Query: 4   MEWPGSLDEYSKLINRMNTPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLNLSI 63
           ME     +EY KL+ RMN PRVVIDN VC  +T+VK+DSAR  GILLE+VQ+LTD+NL I
Sbjct: 1   MELSDCSNEYEKLVVRMNMPRVVIDNGVCPNSTVVKIDSARSPGILLESVQLLTDMNLWI 60

Query: 64  QKAYISSDGIWFMDVFHVTDLDGNKLTDEGVISYLEESLATIRCGKPAASNDLTALELTG 123
           +KAYISSDG W MDVFHV+DL+G+KLTDE +I Y+E+S+ T    K      LTALELTG
Sbjct: 61  KKAYISSDGKWNMDVFHVSDLNGDKLTDENLIRYIEKSIETSHYCKTEGYTGLTALELTG 120

Query: 124 TDRVGLLSEVFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIK-DSQKIDTIVG 183
           TDRVGLLSEVFAVLA+L+CDVVEAK WTHNGRIAS+IYVKD NSG+PI  DS ++  + G
Sbjct: 121 TDRVGLLSEVFAVLADLECDVVEAKAWTHNGRIASMIYVKDGNSGTPIDGDSDRVQRVEG 180

Query: 184 RLRNVLKGDDDILY-AKTSVAM-TVTHTERRLHQMMFADRDYERKPVLQHSEDSPVVTVQ 243
           +LRN+LK DD      +T V+    TH ERRLHQ MF DRDYE+K  +   E SP+V+VQ
Sbjct: 181 QLRNLLKADDGYQNDTRTCVSYGGNTHMERRLHQRMFMDRDYEKKFDI---EKSPIVSVQ 240

Query: 244 NLVERGYSVVNVQCKDRMKLLFDVVCTMTDMDYVVFHGTVATTSHRAYLEFYIRHTDGTP 303
           NL +RGYSVVN+QCKDRMKLLFDVVCT+TDM Y+VFH  + T    A+LEFY+RH+DG P
Sbjct: 241 NLPKRGYSVVNLQCKDRMKLLFDVVCTLTDMAYIVFHAAIRTVGETAFLEFYVRHSDGHP 300

Query: 304 ISSEAERQRVIQCLQASVERRTSRGVRLELRTTDRPCLLADVTRTFRENGLNVTRAEVST 363
           +SSE ERQR+IQCLQA++ERRT +GVRLEL T DRP LLA+VTR  RENGLN+ RAE+ST
Sbjct: 301 VSSEPERQRLIQCLQAAIERRTVKGVRLELCTADRPGLLAEVTRILRENGLNIARAEIST 360

Query: 364 SADVALNLFYVTDGHGSAVDTKTIDSVREKIGMSSLKVKE-LPLISQQKTEGEE------ 423
              +A N+FYVTD +G+ +D + I S+REKIG+  L VKE  P+  ++  E E+      
Sbjct: 361 KDSIARNVFYVTDANGNLIDPEIIKSIREKIGIDDLSVKEPFPISCREAVEKEQHIEPQD 420

Query: 424 -QGAGVGGAVLLSLGSILRRNLYNLGLIRSY 444
            QG   GG VL+SLGS++ RNLY+LGLI+SY
Sbjct: 421 HQGRYGGGTVLVSLGSLVMRNLYHLGLIKSY 448

BLAST of Bhi12G000337 vs. ExPASy Swiss-Prot
Match: Q8LJW3 (ACT domain-containing protein ACR4 OS=Arabidopsis thaliana OX=3702 GN=ACR4 PE=2 SV=1)

HSP 1 Score: 447.6 bits (1150), Expect = 1.7e-124
Identity = 239/446 (53.59%), Postives = 304/446 (68.16%), Query Frame = 0

Query: 11  DEYSKLINRMNTPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLNLSIQKAYISS 70
           +EY KLI RMN PRVVIDN  C+ AT+++VDSA  +GILLE VQ+LTDLNL+I KAYISS
Sbjct: 9   NEYEKLIRRMNPPRVVIDNDSCKKATVIRVDSANEYGILLEVVQILTDLNLTITKAYISS 68

Query: 71  DGIWFMDVFHVTDLDGNKLTDEGVISYLEESLATIRCGKPA-------ASNDLTALELTG 130
           DG WFMDVF+VTD DGNK+TDE V+ Y+++SL    C   +        S D T +ELTG
Sbjct: 69  DGGWFMDVFNVTDQDGNKVTDEVVLDYIQKSLGPEACFSTSMRSVGVIPSTDSTVIELTG 128

Query: 131 TDRVGLLSEVFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDSQKIDTIVGR 190
            DR GLLSE+ AVL  L+C V+ A++WTHN R A+++ V D  +G  I D +++  I   
Sbjct: 129 CDRPGLLSELSAVLTHLKCSVLNAEIWTHNTRAAAVMQVTDDLTGCGISDPERLSRIKNL 188

Query: 191 LRNVLKGDDDILYAKTSVAMTVTHTERRLHQMMFADRDYERKPV-----LQHSEDSPVVT 250
           LRNVLKG +    AKT V+    HT+RRLHQMMF DRDYE + V     +Q     P V 
Sbjct: 189 LRNVLKGSNTPREAKTVVSHGEVHTDRRLHQMMFEDRDYEHRLVDDDSSIQDERQRPDVC 248

Query: 251 VQNLVERGYSVVNVQCKDRMKLLFDVVCTMTDMDYVVFHGTVATTSHRAYLEFYIRHTDG 310
           V N +++ YSVV V+CKDR KLLFD VCT+TDM YVVFHG+V T    A+ E+Y+RH DG
Sbjct: 249 VDNWLDKDYSVVTVRCKDRPKLLFDTVCTLTDMQYVVFHGSVDTEGTEAFQEYYVRHIDG 308

Query: 311 TPISSEAERQRVIQCLQASVERRTSRGVRLELRTTDRPCLLADVTRTFRENGLNVTRAEV 370
           +P+ SEAE+QRVIQCL+A+++RR S G++LEL TTDR  LL++VTR FREN L VTRAEV
Sbjct: 309 SPVKSEAEKQRVIQCLEAAIKRRVSEGLKLELCTTDRVGLLSNVTRIFRENSLTVTRAEV 368

Query: 371 STSADVALNLFYVTDGHGSAVDTKTIDSVREKIGMSSLKVKELPLISQQKTEGEEQGAGV 430
            T    ALN FYV+D  G ++D KTIDS+R+ IG + LKVK  P   QQ+ +     +  
Sbjct: 369 KTKGGKALNTFYVSDASGYSIDAKTIDSIRQTIGQTILKVKNNPQEQQQRQKSPSHESPT 428

Query: 431 GGAVLLSLGSILRRNLYNLGLIRSYS 445
                L  G    ++  N GL+RSYS
Sbjct: 429 R---FLFGGLFKSKSFVNFGLVRSYS 451

BLAST of Bhi12G000337 vs. ExPASy Swiss-Prot
Match: Q9ZPQ8 (ACT domain-containing protein ACR5 OS=Arabidopsis thaliana OX=3702 GN=ACR5 PE=2 SV=2)

HSP 1 Score: 409.1 bits (1050), Expect = 6.5e-113
Identity = 232/452 (51.33%), Postives = 297/452 (65.71%), Query Frame = 0

Query: 11  DEYSKLINRMNTPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLNLSIQKAYISS 70
           DE +K I R+N PRVVIDN VC+  T++KVDSA +HGILLE VQVLT+LNL+I+KAYISS
Sbjct: 13  DEIAKFIRRVNPPRVVIDNEVCKDVTVIKVDSANKHGILLEVVQVLTELNLTIKKAYISS 72

Query: 71  DGIWFMDVFHVTDLDGNKLTDEGVISYLEESLA--TIRCGKPA--------ASNDLTALE 130
           DG WFMDVF+VTD DGNK+TDE V+ Y+ +SL      C  P+         S D T +E
Sbjct: 73  DGGWFMDVFNVTDQDGNKVTDEIVLEYIRKSLGPDESSCFSPSMRSTIGVKQSVDYTVVE 132

Query: 131 LTGTDRVGLLSEVFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDSQKIDTI 190
           LTGTDR GLLSE+ AVL +LQC+VV A++WTH  + A+++ V D  + S I D +++  I
Sbjct: 133 LTGTDRPGLLSELCAVLMDLQCNVVNAEIWTHRAKAAAVLQVTDEETCSAITDPERLSKI 192

Query: 191 VGRLRNVLKGDDD---ILYAKTSV--AMTVTHTERRLHQMMFADRDYERKPVLQHSEDS- 250
              L  VL G          KT+V  A+  THT+R+LHQ+MFADRDY+        ED  
Sbjct: 193 RKLLGYVLTGGSSGRRFREPKTTVSSALNETHTDRKLHQLMFADRDYDEWENNVDDEDKC 252

Query: 251 ----PVVTVQNLVERGYSVVNVQCKDRMKLLFDVVCTMTDMDYVVFHGTVATTSHRAYLE 310
               P V V NL +  YS+V ++CKDR KLLFD V T+TDM+YVV H ++     +AY E
Sbjct: 253 GRVIPDVDVSNLHDLDYSIVMIKCKDRPKLLFDTVFTLTDMNYVVSHASIDAEGPQAYQE 312

Query: 311 FYIRHTDGTPISSEAERQRVIQCLQASVERRTSRGVRLELRTTDRPCLLADVTRTFRENG 370
           +YIRHTDG+P+ SEAERQRVI+CL+A+++RR S G++LEL T+DR  LL+DVTR FREN 
Sbjct: 313 YYIRHTDGSPVKSEAERQRVIKCLKAAIQRRVSEGLKLELCTSDRVGLLSDVTRIFRENS 372

Query: 371 LNVTRAEVSTSADVALNLFYVTDGHGSAVDTKTIDSVREKIGMSSLKVKELPLISQQKTE 430
           L VTRAEV T  D ALN FYV D  G  VDTKTI+S+R+ IG + L+VK     ++   +
Sbjct: 373 LTVTRAEVKTKGDKALNTFYVRDASGYQVDTKTIESIRQVIGQTILQVKGGNTDAKPSPQ 432

Query: 431 GEEQGAGVGGAVLLSLGSILRRNLYNLGLIRS 443
               G           G    R+  N GLIRS
Sbjct: 433 DSPTG--------FLFGVFKSRSFVNFGLIRS 456

BLAST of Bhi12G000337 vs. ExPASy Swiss-Prot
Match: Q9SGA0 (ACT domain-containing protein ACR6 OS=Arabidopsis thaliana OX=3702 GN=ACR6 PE=1 SV=1)

HSP 1 Score: 404.1 bits (1037), Expect = 2.1e-111
Identity = 214/399 (53.63%), Postives = 281/399 (70.43%), Query Frame = 0

Query: 11  DEYSKLINRMNTPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLNLSIQKAYISS 70
           DEY+KLI RMN PRVVIDN   + AT+++VDS  +HG LLE VQVLTD+NL I+KAYISS
Sbjct: 4   DEYAKLIRRMNPPRVVIDNNASDDATVIQVDSVNKHGTLLEVVQVLTDMNLVIKKAYISS 63

Query: 71  DGIWFMDVFHVTDLDGNKLTDEGVISYLEESLAT--------IRCGKPAASND-LTALEL 130
           DG WFMDVF V D DGNK+ D  V+ Y+++ + +        +R        D  T++EL
Sbjct: 64  DGGWFMDVFKVIDQDGNKIRDTQVLDYIQKRIESNAGWFIPPLRSSVGVMPTDEYTSIEL 123

Query: 131 TGTDRVGLLSEVFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDSQKIDTIV 190
            GTDR GLLSEV AVL +L C+VV A++WTHN R A++I+V D ++ S I D  ++ TI 
Sbjct: 124 AGTDRPGLLSEVSAVLTDLHCNVVNAEIWTHNTRAAAVIHVTDNSTHSAITDPIRLSTIK 183

Query: 191 GRLRNVLKGDDDILYAKTSVAMTVTHTERRLHQMMFADRDYERKPVLQHSEDSPVVTVQN 250
             L NV++ +     AKT  + + TH ERRLHQ+MF DRDYE     + S   P VT+ N
Sbjct: 184 ELLCNVVRTNSGSRAAKTVFSCSDTHRERRLHQIMFDDRDYEGVKRARTSASRPSVTLMN 243

Query: 251 LVERGYSVVNVQCKDRMKLLFDVVCTMTDMDYVVFHGTVATTSHRAYLEFYIRHTDGTPI 310
            +E+ Y+VV ++ KDR KL+FDVVCT+TDM YVVFHG V+T    AY EFYIRH DG PI
Sbjct: 244 -IEKDYTVVTMRSKDRPKLVFDVVCTLTDMQYVVFHGMVSTEPVEAYQEFYIRHVDGLPI 303

Query: 311 SSEAERQRVIQCLQASVERRTSRGVRLELRTTDRPCLLADVTRTFRENGLNVTRAEVSTS 370
           +SEAE++RVIQCL+A++ERR S G+ LEL   DR  LL+D+TRTFREN L + RAE+ST 
Sbjct: 304 NSEAEQERVIQCLEAAIERRASEGLELELSAEDRVGLLSDITRTFRENSLTIVRAEISTR 363

Query: 371 ADVALNLFYVTDGHGSAVDTKTIDSVREKIGMSSLKVKE 401
              A + FYVTD  G+ V++K ++S+R++IG+S LKVK+
Sbjct: 364 EGKAKDTFYVTDVTGNPVESKIVESIRQQIGVSKLKVKK 401

BLAST of Bhi12G000337 vs. NCBI nr
Match: XP_038876322.1 (ACT domain-containing protein ACR8-like [Benincasa hispida])

HSP 1 Score: 869.0 bits (2244), Expect = 1.8e-248
Identity = 444/444 (100.00%), Postives = 444/444 (100.00%), Query Frame = 0

Query: 1   MVEMEWPGSLDEYSKLINRMNTPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLN 60
           MVEMEWPGSLDEYSKLINRMNTPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLN
Sbjct: 1   MVEMEWPGSLDEYSKLINRMNTPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLN 60

Query: 61  LSIQKAYISSDGIWFMDVFHVTDLDGNKLTDEGVISYLEESLATIRCGKPAASNDLTALE 120
           LSIQKAYISSDGIWFMDVFHVTDLDGNKLTDEGVISYLEESLATIRCGKPAASNDLTALE
Sbjct: 61  LSIQKAYISSDGIWFMDVFHVTDLDGNKLTDEGVISYLEESLATIRCGKPAASNDLTALE 120

Query: 121 LTGTDRVGLLSEVFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDSQKIDTI 180
           LTGTDRVGLLSEVFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDSQKIDTI
Sbjct: 121 LTGTDRVGLLSEVFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDSQKIDTI 180

Query: 181 VGRLRNVLKGDDDILYAKTSVAMTVTHTERRLHQMMFADRDYERKPVLQHSEDSPVVTVQ 240
           VGRLRNVLKGDDDILYAKTSVAMTVTHTERRLHQMMFADRDYERKPVLQHSEDSPVVTVQ
Sbjct: 181 VGRLRNVLKGDDDILYAKTSVAMTVTHTERRLHQMMFADRDYERKPVLQHSEDSPVVTVQ 240

Query: 241 NLVERGYSVVNVQCKDRMKLLFDVVCTMTDMDYVVFHGTVATTSHRAYLEFYIRHTDGTP 300
           NLVERGYSVVNVQCKDRMKLLFDVVCTMTDMDYVVFHGTVATTSHRAYLEFYIRHTDGTP
Sbjct: 241 NLVERGYSVVNVQCKDRMKLLFDVVCTMTDMDYVVFHGTVATTSHRAYLEFYIRHTDGTP 300

Query: 301 ISSEAERQRVIQCLQASVERRTSRGVRLELRTTDRPCLLADVTRTFRENGLNVTRAEVST 360
           ISSEAERQRVIQCLQASVERRTSRGVRLELRTTDRPCLLADVTRTFRENGLNVTRAEVST
Sbjct: 301 ISSEAERQRVIQCLQASVERRTSRGVRLELRTTDRPCLLADVTRTFRENGLNVTRAEVST 360

Query: 361 SADVALNLFYVTDGHGSAVDTKTIDSVREKIGMSSLKVKELPLISQQKTEGEEQGAGVGG 420
           SADVALNLFYVTDGHGSAVDTKTIDSVREKIGMSSLKVKELPLISQQKTEGEEQGAGVGG
Sbjct: 361 SADVALNLFYVTDGHGSAVDTKTIDSVREKIGMSSLKVKELPLISQQKTEGEEQGAGVGG 420

Query: 421 AVLLSLGSILRRNLYNLGLIRSYS 445
           AVLLSLGSILRRNLYNLGLIRSYS
Sbjct: 421 AVLLSLGSILRRNLYNLGLIRSYS 444

BLAST of Bhi12G000337 vs. NCBI nr
Match: XP_008437163.1 (PREDICTED: ACT domain-containing protein ACR8-like [Cucumis melo] >KAA0042840.1 ACT domain-containing protein ACR8-like [Cucumis melo var. makuwa])

HSP 1 Score: 833.9 bits (2153), Expect = 6.3e-238
Identity = 421/444 (94.82%), Postives = 434/444 (97.75%), Query Frame = 0

Query: 1   MVEMEWPGSLDEYSKLINRMNTPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLN 60
           MV++EWPGSLDEYSKLINRMNTPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLN
Sbjct: 5   MVDIEWPGSLDEYSKLINRMNTPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLN 64

Query: 61  LSIQKAYISSDGIWFMDVFHVTDLDGNKLTDEGVISYLEESLATIRCGKPAASNDLTALE 120
           LSIQKAYISSDGIWFMDVFHVTDL+GNKLTDEGVISYLE+SLATI CGKPA SNDLTALE
Sbjct: 65  LSIQKAYISSDGIWFMDVFHVTDLEGNKLTDEGVISYLEQSLATIHCGKPATSNDLTALE 124

Query: 121 LTGTDRVGLLSEVFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDSQKIDTI 180
           LTGTDRVGLLSEVFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDS+KIDTI
Sbjct: 125 LTGTDRVGLLSEVFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDSEKIDTI 184

Query: 181 VGRLRNVLKGDDDILYAKTSVAMTVTHTERRLHQMMFADRDYERKPVLQHSEDSPVVTVQ 240
           VGRLRNVLKGDDDILYAKTSV+MTVTHTERRLHQMMFADRDYERKPVLQHSEDSPVVTVQ
Sbjct: 185 VGRLRNVLKGDDDILYAKTSVSMTVTHTERRLHQMMFADRDYERKPVLQHSEDSPVVTVQ 244

Query: 241 NLVERGYSVVNVQCKDRMKLLFDVVCTMTDMDYVVFHGTVATTSHRAYLEFYIRHTDGTP 300
           NLVERGYSVVNVQCKDRMKLLFDV+CTMTDMDYVVFHGT+ T+ HRAYLEFYIRHTDGTP
Sbjct: 245 NLVERGYSVVNVQCKDRMKLLFDVICTMTDMDYVVFHGTITTSRHRAYLEFYIRHTDGTP 304

Query: 301 ISSEAERQRVIQCLQASVERRTSRGVRLELRTTDRPCLLADVTRTFRENGLNVTRAEVST 360
           ISSEAERQRVIQCLQAS+ERRTSRGVRLEL TTDRPCLLADVTRTFRENGLNVTRAEVST
Sbjct: 305 ISSEAERQRVIQCLQASIERRTSRGVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVST 364

Query: 361 SADVALNLFYVTDGHGSAVDTKTIDSVREKIGMSSLKVKELPLISQQKTEGEEQGAGVGG 420
           S +VALNLFYVTDGHGSA DTK IDSVREKIG+S+LKVKELPLISQQKTEGEEQ A VGG
Sbjct: 365 SQEVALNLFYVTDGHGSAADTKMIDSVREKIGVSNLKVKELPLISQQKTEGEEQAASVGG 424

Query: 421 AVLLSLGSILRRNLYNLGLIRSYS 445
           AVLLSLGSILRRNLYNLGLIRSYS
Sbjct: 425 AVLLSLGSILRRNLYNLGLIRSYS 448

BLAST of Bhi12G000337 vs. NCBI nr
Match: XP_004147566.1 (ACT domain-containing protein ACR8 [Cucumis sativus] >KGN50186.1 hypothetical protein Csa_000586 [Cucumis sativus])

HSP 1 Score: 829.7 bits (2142), Expect = 1.2e-236
Identity = 417/444 (93.92%), Postives = 433/444 (97.52%), Query Frame = 0

Query: 1   MVEMEWPGSLDEYSKLINRMNTPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLN 60
           MVE+EWPGSLDEYSKLINRMNTPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLN
Sbjct: 5   MVEIEWPGSLDEYSKLINRMNTPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLN 64

Query: 61  LSIQKAYISSDGIWFMDVFHVTDLDGNKLTDEGVISYLEESLATIRCGKPAASNDLTALE 120
           LSIQKAYISSDGIWFMDVFHVTDL+GNKLTDEGVISYLE+SLATI CGKPA SNDLTALE
Sbjct: 65  LSIQKAYISSDGIWFMDVFHVTDLEGNKLTDEGVISYLEQSLATIHCGKPATSNDLTALE 124

Query: 121 LTGTDRVGLLSEVFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDSQKIDTI 180
           LTGTDRVGLLSEVFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIK+S++IDTI
Sbjct: 125 LTGTDRVGLLSEVFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIKESERIDTI 184

Query: 181 VGRLRNVLKGDDDILYAKTSVAMTVTHTERRLHQMMFADRDYERKPVLQHSEDSPVVTVQ 240
           VGRLRNVLKGDDDILYAKTSV+MTVTHTERRLHQMMFADRDYERKPV QH+EDSPVVTVQ
Sbjct: 185 VGRLRNVLKGDDDILYAKTSVSMTVTHTERRLHQMMFADRDYERKPVQQHTEDSPVVTVQ 244

Query: 241 NLVERGYSVVNVQCKDRMKLLFDVVCTMTDMDYVVFHGTVATTSHRAYLEFYIRHTDGTP 300
           NLVERGYSVVN+QCKDRMKLLFDV+CTMTDMDYVVFHGT+ T+ HRAYLEFYIRHTDGTP
Sbjct: 245 NLVERGYSVVNIQCKDRMKLLFDVICTMTDMDYVVFHGTITTSRHRAYLEFYIRHTDGTP 304

Query: 301 ISSEAERQRVIQCLQASVERRTSRGVRLELRTTDRPCLLADVTRTFRENGLNVTRAEVST 360
           ISSEAERQRVIQCLQAS+ERRTSRGVRLEL TTDRPCLLADVTRTFRENGLNVTRAEVST
Sbjct: 305 ISSEAERQRVIQCLQASIERRTSRGVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVST 364

Query: 361 SADVALNLFYVTDGHGSAVDTKTIDSVREKIGMSSLKVKELPLISQQKTEGEEQGAGVGG 420
           S +VALNLFYVTDGHGSA DTK IDSVREKIGMS+LKVKELPL+SQQKTEGEEQ A VGG
Sbjct: 365 SQEVALNLFYVTDGHGSAADTKMIDSVREKIGMSNLKVKELPLVSQQKTEGEEQAASVGG 424

Query: 421 AVLLSLGSILRRNLYNLGLIRSYS 445
           AVLLSLGSILRRNLYNLGLIRSYS
Sbjct: 425 AVLLSLGSILRRNLYNLGLIRSYS 448

BLAST of Bhi12G000337 vs. NCBI nr
Match: XP_022957743.1 (ACT domain-containing protein ACR8-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 816.6 bits (2108), Expect = 1.0e-232
Identity = 415/444 (93.47%), Postives = 426/444 (95.95%), Query Frame = 0

Query: 1   MVEMEWPGSLDEYSKLINRMNTPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLN 60
           MVEMEWPGSLDEYSKLINR+N PRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLN
Sbjct: 1   MVEMEWPGSLDEYSKLINRINIPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLN 60

Query: 61  LSIQKAYISSDGIWFMDVFHVTDLDGNKLTDEGVISYLEESLATIRCGKPAASNDLTALE 120
           LSIQKAYISSDGIWFMDVFHVTDLDGNKL DEGVISYLE+SLATI CGKPAASNDLTALE
Sbjct: 61  LSIQKAYISSDGIWFMDVFHVTDLDGNKLIDEGVISYLEKSLATIHCGKPAASNDLTALE 120

Query: 121 LTGTDRVGLLSEVFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDSQKIDTI 180
           LTGTDRVGLLSEVFAVLAEL+CDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDSQKIDTI
Sbjct: 121 LTGTDRVGLLSEVFAVLAELECDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDSQKIDTI 180

Query: 181 VGRLRNVLKGDDDILYAKTSVAMTVTHTERRLHQMMFADRDYERKPVLQHSEDSPVVTVQ 240
           VGRLRNVLKG DDILYAKTSV+MTVTHTERRLHQMMFADRDYERKPVLQHS+DSPVVTVQ
Sbjct: 181 VGRLRNVLKGQDDILYAKTSVSMTVTHTERRLHQMMFADRDYERKPVLQHSDDSPVVTVQ 240

Query: 241 NLVERGYSVVNVQCKDRMKLLFDVVCTMTDMDYVVFHGTVATTSHRAYLEFYIRHTDGTP 300
           N VERGYSVVNVQCKDRMKLLFDVVCT+TDMDYVVFHGT+ T   RAYLE YIRHTDGTP
Sbjct: 241 NWVERGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAKDRAYLELYIRHTDGTP 300

Query: 301 ISSEAERQRVIQCLQASVERRTSRGVRLELRTTDRPCLLADVTRTFRENGLNVTRAEVST 360
           ISSEAERQRVIQCLQASVERRTS+GVRLELRTTDRPCLLADVTRTFRENGLNVTRAEVST
Sbjct: 301 ISSEAERQRVIQCLQASVERRTSQGVRLELRTTDRPCLLADVTRTFRENGLNVTRAEVST 360

Query: 361 SADVALNLFYVTDGHGSAVDTKTIDSVREKIGMSSLKVKELPLISQQKTEGEEQGAGVGG 420
           SADVALNLFYVTD  G+A D KTI+SVREKIG+S LKVKELPLISQQKTEGEEQG GVGG
Sbjct: 361 SADVALNLFYVTDALGNAADVKTINSVREKIGLSCLKVKELPLISQQKTEGEEQGVGVGG 420

Query: 421 AVLLSLGSILRRNLYNLGLIRSYS 445
            VL SLGSILRRNLYNLGLIRSYS
Sbjct: 421 TVLFSLGSILRRNLYNLGLIRSYS 444

BLAST of Bhi12G000337 vs. NCBI nr
Match: KAG6606150.1 (ACT domain-containing protein ACR8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 814.3 bits (2102), Expect = 5.1e-232
Identity = 415/444 (93.47%), Postives = 425/444 (95.72%), Query Frame = 0

Query: 1   MVEMEWPGSLDEYSKLINRMNTPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLN 60
           MVEMEWPGSLDEYSKLINRMN PRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLN
Sbjct: 1   MVEMEWPGSLDEYSKLINRMNIPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLN 60

Query: 61  LSIQKAYISSDGIWFMDVFHVTDLDGNKLTDEGVISYLEESLATIRCGKPAASNDLTALE 120
           LSIQKAYISSDGIWFMDVFHVTDLDGNKL DEGVISYLE+SLATI CGKPAASNDLTALE
Sbjct: 61  LSIQKAYISSDGIWFMDVFHVTDLDGNKLIDEGVISYLEKSLATIHCGKPAASNDLTALE 120

Query: 121 LTGTDRVGLLSEVFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDSQKIDTI 180
           LTGTDRVGLLSEVFAVLAEL+CDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDS KIDTI
Sbjct: 121 LTGTDRVGLLSEVFAVLAELECDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDSLKIDTI 180

Query: 181 VGRLRNVLKGDDDILYAKTSVAMTVTHTERRLHQMMFADRDYERKPVLQHSEDSPVVTVQ 240
           VGRLRNVLKG DDILYAKTSV+MTVTHTERRLHQMMFADRDYERKPVLQHS+DSPVVTVQ
Sbjct: 181 VGRLRNVLKGQDDILYAKTSVSMTVTHTERRLHQMMFADRDYERKPVLQHSDDSPVVTVQ 240

Query: 241 NLVERGYSVVNVQCKDRMKLLFDVVCTMTDMDYVVFHGTVATTSHRAYLEFYIRHTDGTP 300
           N VER YSVVNVQCKDRMKLLFDVVCT+TDMDYVVFHGT+ T   RAYLE YIRHTDGTP
Sbjct: 241 NWVERDYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAKDRAYLELYIRHTDGTP 300

Query: 301 ISSEAERQRVIQCLQASVERRTSRGVRLELRTTDRPCLLADVTRTFRENGLNVTRAEVST 360
           ISSEAERQRVIQCLQASVERRTS+GVRLELRTTDRPCLLADVTRTFRENGLNVTRAEVST
Sbjct: 301 ISSEAERQRVIQCLQASVERRTSQGVRLELRTTDRPCLLADVTRTFRENGLNVTRAEVST 360

Query: 361 SADVALNLFYVTDGHGSAVDTKTIDSVREKIGMSSLKVKELPLISQQKTEGEEQGAGVGG 420
           SADVALNLFYVTD  G+A D KTI+SVREKIG+S LKVKELPLISQQKTEGEEQG GVGG
Sbjct: 361 SADVALNLFYVTDALGNAADVKTINSVREKIGLSCLKVKELPLISQQKTEGEEQGVGVGG 420

Query: 421 AVLLSLGSILRRNLYNLGLIRSYS 445
            VLLSLGSILRRNLYNLGLIRSYS
Sbjct: 421 TVLLSLGSILRRNLYNLGLIRSYS 444

BLAST of Bhi12G000337 vs. ExPASy TrEMBL
Match: A0A5A7THS3 (ACT domain-containing protein ACR8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003490 PE=4 SV=1)

HSP 1 Score: 833.9 bits (2153), Expect = 3.0e-238
Identity = 421/444 (94.82%), Postives = 434/444 (97.75%), Query Frame = 0

Query: 1   MVEMEWPGSLDEYSKLINRMNTPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLN 60
           MV++EWPGSLDEYSKLINRMNTPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLN
Sbjct: 5   MVDIEWPGSLDEYSKLINRMNTPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLN 64

Query: 61  LSIQKAYISSDGIWFMDVFHVTDLDGNKLTDEGVISYLEESLATIRCGKPAASNDLTALE 120
           LSIQKAYISSDGIWFMDVFHVTDL+GNKLTDEGVISYLE+SLATI CGKPA SNDLTALE
Sbjct: 65  LSIQKAYISSDGIWFMDVFHVTDLEGNKLTDEGVISYLEQSLATIHCGKPATSNDLTALE 124

Query: 121 LTGTDRVGLLSEVFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDSQKIDTI 180
           LTGTDRVGLLSEVFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDS+KIDTI
Sbjct: 125 LTGTDRVGLLSEVFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDSEKIDTI 184

Query: 181 VGRLRNVLKGDDDILYAKTSVAMTVTHTERRLHQMMFADRDYERKPVLQHSEDSPVVTVQ 240
           VGRLRNVLKGDDDILYAKTSV+MTVTHTERRLHQMMFADRDYERKPVLQHSEDSPVVTVQ
Sbjct: 185 VGRLRNVLKGDDDILYAKTSVSMTVTHTERRLHQMMFADRDYERKPVLQHSEDSPVVTVQ 244

Query: 241 NLVERGYSVVNVQCKDRMKLLFDVVCTMTDMDYVVFHGTVATTSHRAYLEFYIRHTDGTP 300
           NLVERGYSVVNVQCKDRMKLLFDV+CTMTDMDYVVFHGT+ T+ HRAYLEFYIRHTDGTP
Sbjct: 245 NLVERGYSVVNVQCKDRMKLLFDVICTMTDMDYVVFHGTITTSRHRAYLEFYIRHTDGTP 304

Query: 301 ISSEAERQRVIQCLQASVERRTSRGVRLELRTTDRPCLLADVTRTFRENGLNVTRAEVST 360
           ISSEAERQRVIQCLQAS+ERRTSRGVRLEL TTDRPCLLADVTRTFRENGLNVTRAEVST
Sbjct: 305 ISSEAERQRVIQCLQASIERRTSRGVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVST 364

Query: 361 SADVALNLFYVTDGHGSAVDTKTIDSVREKIGMSSLKVKELPLISQQKTEGEEQGAGVGG 420
           S +VALNLFYVTDGHGSA DTK IDSVREKIG+S+LKVKELPLISQQKTEGEEQ A VGG
Sbjct: 365 SQEVALNLFYVTDGHGSAADTKMIDSVREKIGVSNLKVKELPLISQQKTEGEEQAASVGG 424

Query: 421 AVLLSLGSILRRNLYNLGLIRSYS 445
           AVLLSLGSILRRNLYNLGLIRSYS
Sbjct: 425 AVLLSLGSILRRNLYNLGLIRSYS 448

BLAST of Bhi12G000337 vs. ExPASy TrEMBL
Match: A0A1S3ATY2 (ACT domain-containing protein ACR8-like OS=Cucumis melo OX=3656 GN=LOC103482670 PE=4 SV=1)

HSP 1 Score: 833.9 bits (2153), Expect = 3.0e-238
Identity = 421/444 (94.82%), Postives = 434/444 (97.75%), Query Frame = 0

Query: 1   MVEMEWPGSLDEYSKLINRMNTPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLN 60
           MV++EWPGSLDEYSKLINRMNTPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLN
Sbjct: 5   MVDIEWPGSLDEYSKLINRMNTPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLN 64

Query: 61  LSIQKAYISSDGIWFMDVFHVTDLDGNKLTDEGVISYLEESLATIRCGKPAASNDLTALE 120
           LSIQKAYISSDGIWFMDVFHVTDL+GNKLTDEGVISYLE+SLATI CGKPA SNDLTALE
Sbjct: 65  LSIQKAYISSDGIWFMDVFHVTDLEGNKLTDEGVISYLEQSLATIHCGKPATSNDLTALE 124

Query: 121 LTGTDRVGLLSEVFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDSQKIDTI 180
           LTGTDRVGLLSEVFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDS+KIDTI
Sbjct: 125 LTGTDRVGLLSEVFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDSEKIDTI 184

Query: 181 VGRLRNVLKGDDDILYAKTSVAMTVTHTERRLHQMMFADRDYERKPVLQHSEDSPVVTVQ 240
           VGRLRNVLKGDDDILYAKTSV+MTVTHTERRLHQMMFADRDYERKPVLQHSEDSPVVTVQ
Sbjct: 185 VGRLRNVLKGDDDILYAKTSVSMTVTHTERRLHQMMFADRDYERKPVLQHSEDSPVVTVQ 244

Query: 241 NLVERGYSVVNVQCKDRMKLLFDVVCTMTDMDYVVFHGTVATTSHRAYLEFYIRHTDGTP 300
           NLVERGYSVVNVQCKDRMKLLFDV+CTMTDMDYVVFHGT+ T+ HRAYLEFYIRHTDGTP
Sbjct: 245 NLVERGYSVVNVQCKDRMKLLFDVICTMTDMDYVVFHGTITTSRHRAYLEFYIRHTDGTP 304

Query: 301 ISSEAERQRVIQCLQASVERRTSRGVRLELRTTDRPCLLADVTRTFRENGLNVTRAEVST 360
           ISSEAERQRVIQCLQAS+ERRTSRGVRLEL TTDRPCLLADVTRTFRENGLNVTRAEVST
Sbjct: 305 ISSEAERQRVIQCLQASIERRTSRGVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVST 364

Query: 361 SADVALNLFYVTDGHGSAVDTKTIDSVREKIGMSSLKVKELPLISQQKTEGEEQGAGVGG 420
           S +VALNLFYVTDGHGSA DTK IDSVREKIG+S+LKVKELPLISQQKTEGEEQ A VGG
Sbjct: 365 SQEVALNLFYVTDGHGSAADTKMIDSVREKIGVSNLKVKELPLISQQKTEGEEQAASVGG 424

Query: 421 AVLLSLGSILRRNLYNLGLIRSYS 445
           AVLLSLGSILRRNLYNLGLIRSYS
Sbjct: 425 AVLLSLGSILRRNLYNLGLIRSYS 448

BLAST of Bhi12G000337 vs. ExPASy TrEMBL
Match: A0A0A0KR33 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G157930 PE=4 SV=1)

HSP 1 Score: 829.7 bits (2142), Expect = 5.7e-237
Identity = 417/444 (93.92%), Postives = 433/444 (97.52%), Query Frame = 0

Query: 1   MVEMEWPGSLDEYSKLINRMNTPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLN 60
           MVE+EWPGSLDEYSKLINRMNTPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLN
Sbjct: 5   MVEIEWPGSLDEYSKLINRMNTPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLN 64

Query: 61  LSIQKAYISSDGIWFMDVFHVTDLDGNKLTDEGVISYLEESLATIRCGKPAASNDLTALE 120
           LSIQKAYISSDGIWFMDVFHVTDL+GNKLTDEGVISYLE+SLATI CGKPA SNDLTALE
Sbjct: 65  LSIQKAYISSDGIWFMDVFHVTDLEGNKLTDEGVISYLEQSLATIHCGKPATSNDLTALE 124

Query: 121 LTGTDRVGLLSEVFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDSQKIDTI 180
           LTGTDRVGLLSEVFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIK+S++IDTI
Sbjct: 125 LTGTDRVGLLSEVFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIKESERIDTI 184

Query: 181 VGRLRNVLKGDDDILYAKTSVAMTVTHTERRLHQMMFADRDYERKPVLQHSEDSPVVTVQ 240
           VGRLRNVLKGDDDILYAKTSV+MTVTHTERRLHQMMFADRDYERKPV QH+EDSPVVTVQ
Sbjct: 185 VGRLRNVLKGDDDILYAKTSVSMTVTHTERRLHQMMFADRDYERKPVQQHTEDSPVVTVQ 244

Query: 241 NLVERGYSVVNVQCKDRMKLLFDVVCTMTDMDYVVFHGTVATTSHRAYLEFYIRHTDGTP 300
           NLVERGYSVVN+QCKDRMKLLFDV+CTMTDMDYVVFHGT+ T+ HRAYLEFYIRHTDGTP
Sbjct: 245 NLVERGYSVVNIQCKDRMKLLFDVICTMTDMDYVVFHGTITTSRHRAYLEFYIRHTDGTP 304

Query: 301 ISSEAERQRVIQCLQASVERRTSRGVRLELRTTDRPCLLADVTRTFRENGLNVTRAEVST 360
           ISSEAERQRVIQCLQAS+ERRTSRGVRLEL TTDRPCLLADVTRTFRENGLNVTRAEVST
Sbjct: 305 ISSEAERQRVIQCLQASIERRTSRGVRLELCTTDRPCLLADVTRTFRENGLNVTRAEVST 364

Query: 361 SADVALNLFYVTDGHGSAVDTKTIDSVREKIGMSSLKVKELPLISQQKTEGEEQGAGVGG 420
           S +VALNLFYVTDGHGSA DTK IDSVREKIGMS+LKVKELPL+SQQKTEGEEQ A VGG
Sbjct: 365 SQEVALNLFYVTDGHGSAADTKMIDSVREKIGMSNLKVKELPLVSQQKTEGEEQAASVGG 424

Query: 421 AVLLSLGSILRRNLYNLGLIRSYS 445
           AVLLSLGSILRRNLYNLGLIRSYS
Sbjct: 425 AVLLSLGSILRRNLYNLGLIRSYS 448

BLAST of Bhi12G000337 vs. ExPASy TrEMBL
Match: A0A6J1H146 (ACT domain-containing protein ACR8-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459196 PE=4 SV=1)

HSP 1 Score: 816.6 bits (2108), Expect = 5.0e-233
Identity = 415/444 (93.47%), Postives = 426/444 (95.95%), Query Frame = 0

Query: 1   MVEMEWPGSLDEYSKLINRMNTPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLN 60
           MVEMEWPGSLDEYSKLINR+N PRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLN
Sbjct: 1   MVEMEWPGSLDEYSKLINRINIPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLN 60

Query: 61  LSIQKAYISSDGIWFMDVFHVTDLDGNKLTDEGVISYLEESLATIRCGKPAASNDLTALE 120
           LSIQKAYISSDGIWFMDVFHVTDLDGNKL DEGVISYLE+SLATI CGKPAASNDLTALE
Sbjct: 61  LSIQKAYISSDGIWFMDVFHVTDLDGNKLIDEGVISYLEKSLATIHCGKPAASNDLTALE 120

Query: 121 LTGTDRVGLLSEVFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDSQKIDTI 180
           LTGTDRVGLLSEVFAVLAEL+CDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDSQKIDTI
Sbjct: 121 LTGTDRVGLLSEVFAVLAELECDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDSQKIDTI 180

Query: 181 VGRLRNVLKGDDDILYAKTSVAMTVTHTERRLHQMMFADRDYERKPVLQHSEDSPVVTVQ 240
           VGRLRNVLKG DDILYAKTSV+MTVTHTERRLHQMMFADRDYERKPVLQHS+DSPVVTVQ
Sbjct: 181 VGRLRNVLKGQDDILYAKTSVSMTVTHTERRLHQMMFADRDYERKPVLQHSDDSPVVTVQ 240

Query: 241 NLVERGYSVVNVQCKDRMKLLFDVVCTMTDMDYVVFHGTVATTSHRAYLEFYIRHTDGTP 300
           N VERGYSVVNVQCKDRMKLLFDVVCT+TDMDYVVFHGT+ T   RAYLE YIRHTDGTP
Sbjct: 241 NWVERGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAKDRAYLELYIRHTDGTP 300

Query: 301 ISSEAERQRVIQCLQASVERRTSRGVRLELRTTDRPCLLADVTRTFRENGLNVTRAEVST 360
           ISSEAERQRVIQCLQASVERRTS+GVRLELRTTDRPCLLADVTRTFRENGLNVTRAEVST
Sbjct: 301 ISSEAERQRVIQCLQASVERRTSQGVRLELRTTDRPCLLADVTRTFRENGLNVTRAEVST 360

Query: 361 SADVALNLFYVTDGHGSAVDTKTIDSVREKIGMSSLKVKELPLISQQKTEGEEQGAGVGG 420
           SADVALNLFYVTD  G+A D KTI+SVREKIG+S LKVKELPLISQQKTEGEEQG GVGG
Sbjct: 361 SADVALNLFYVTDALGNAADVKTINSVREKIGLSCLKVKELPLISQQKTEGEEQGVGVGG 420

Query: 421 AVLLSLGSILRRNLYNLGLIRSYS 445
            VL SLGSILRRNLYNLGLIRSYS
Sbjct: 421 TVLFSLGSILRRNLYNLGLIRSYS 444

BLAST of Bhi12G000337 vs. ExPASy TrEMBL
Match: A0A6J1K9T7 (ACT domain-containing protein ACR8-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491389 PE=4 SV=1)

HSP 1 Score: 811.6 bits (2095), Expect = 1.6e-231
Identity = 414/444 (93.24%), Postives = 424/444 (95.50%), Query Frame = 0

Query: 1   MVEMEWPGSLDEYSKLINRMNTPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLN 60
           MVEMEWPGSLDEYSKLINRMN PRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLN
Sbjct: 1   MVEMEWPGSLDEYSKLINRMNIPRVVIDNAVCETATLVKVDSARRHGILLEAVQVLTDLN 60

Query: 61  LSIQKAYISSDGIWFMDVFHVTDLDGNKLTDEGVISYLEESLATIRCGKPAASNDLTALE 120
           LSIQKAYISSDGIWFMDVFHVTDLDGNKL DEGVISYLE+SLATI CGKPAAS DLTALE
Sbjct: 61  LSIQKAYISSDGIWFMDVFHVTDLDGNKLIDEGVISYLEKSLATIHCGKPAASTDLTALE 120

Query: 121 LTGTDRVGLLSEVFAVLAELQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDSQKIDTI 180
           LTGTDRVGLLSEVFAVLAEL+CDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDS KIDTI
Sbjct: 121 LTGTDRVGLLSEVFAVLAELECDVVEAKVWTHNGRIASLIYVKDCNSGSPIKDSLKIDTI 180

Query: 181 VGRLRNVLKGDDDILYAKTSVAMTVTHTERRLHQMMFADRDYERKPVLQHSEDSPVVTVQ 240
           VGRLRNVLKG DDILYAKTSV+MTVTHTERRLHQMMFADRDYERKPVLQHS+DSPVVTVQ
Sbjct: 181 VGRLRNVLKGQDDILYAKTSVSMTVTHTERRLHQMMFADRDYERKPVLQHSDDSPVVTVQ 240

Query: 241 NLVERGYSVVNVQCKDRMKLLFDVVCTMTDMDYVVFHGTVATTSHRAYLEFYIRHTDGTP 300
           N VERGYSVVNVQCKDRMKLLFDVVCT+TDMDYVVFHGT+ T    AYLE YIRHTDGTP
Sbjct: 241 NWVERGYSVVNVQCKDRMKLLFDVVCTLTDMDYVVFHGTITTAKDIAYLELYIRHTDGTP 300

Query: 301 ISSEAERQRVIQCLQASVERRTSRGVRLELRTTDRPCLLADVTRTFRENGLNVTRAEVST 360
           ISSEAERQRVIQCLQASVERRTS+GVRLELRTTDRPCLLADVTRTFRENGLNVTRAEVST
Sbjct: 301 ISSEAERQRVIQCLQASVERRTSQGVRLELRTTDRPCLLADVTRTFRENGLNVTRAEVST 360

Query: 361 SADVALNLFYVTDGHGSAVDTKTIDSVREKIGMSSLKVKELPLISQQKTEGEEQGAGVGG 420
           SADVALNLFYVTD  G+A D KTI+SVREKIG+S LKVKELPLISQQKTEGEEQG GVGG
Sbjct: 361 SADVALNLFYVTDALGNAADVKTINSVREKIGLSCLKVKELPLISQQKTEGEEQGVGVGG 420

Query: 421 AVLLSLGSILRRNLYNLGLIRSYS 445
            VLLSLGSILRRNLYNLGLIRSYS
Sbjct: 421 TVLLSLGSILRRNLYNLGLIRSYS 444