Bhi12G000329 (gene) Wax gourd (B227) v1

Overview
NameBhi12G000329
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Description1-aminocyclopropane-1-carboxylate synthase 1
Locationchr12: 11253080 .. 11257860 (+)
RNA-Seq ExpressionBhi12G000329
SyntenyBhi12G000329
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGTAAAATAAGAAACCCTTAAAAAAAAAAAAAAGAAAAAAGAAAAAAAAGGAAAAAAAATCGGGGTGAATTCATCTCCGCCTTAAAGCCAGAAGAATCGGCGCGGAAAGTACCAATGTAACATAAACATCATAAATTCTTTTATTTTTTTAAAATTCTTTTCGAAGGAAAAATGTTCAAATAATTCAATTTTGTCAATCCTACCCTTCGAGCTTTTTGCTTGCCCTGCCGGTCGCCGGAATTTTCCTCGTCTATCCGGTGAGAGAAGCTCCTCCACCCTCGCCCCTCGCCGGCGGCGGTTTATCTTCGGGTTCTGGTGGATTGAAATGACTCTGCAATGACCCAGACCCGATTCTTCAGAACTCGGAACTCCAAATCTGACAAAGAAGCAGAACCCACCACAAGCGGTGGCGGAGGAGCCGCCATGAGAGTCATAGTGCCTCTGCAAGGCGTCGTTCAAGGAAGAGGCGGCGTCGTTTTGGGCTCTGTTATACCTTGCGTTCTCTTCTACTTTCTTCAGCTCTACTTGAAACGAAACCGGTCCGAACCATCCCCGGACCCTCCTTCCTCGCAGGAGGTGGATTCTTCGGGGAACTCTTCGCCATCGTCGAGTAATCAGTTAGTGGAGCTCTCTGTACTGCCTCGAACATTGTCTCGGACCTTCCTCTCGCCGAGGTCGAGTGGGCCGCTTTGCGTTTCGGGTCGGGCAAATTCGATTTCTAGAGTTGGGGATTCTACGATTTTCGTTGGAATGAGGAGGTTCTTGGAGGACCCATTTGATGAATTGGATAATCCGACGGGGATTATTCAGCTTTCTGTTGCTGAGAATAAGGTGGGTGGCTTTATGCTTTTTGTTGGTTTGAATATGTGTCAGCAAGATTTTTGTGCTCTGTATTTCTCGGTTTCTGTGTTGACGATTGTGGATGTTTAATCAAACGATGAAGTTATCCTCGGACTTGGTGCAAAATTGGCTAACGGAGAATGGAAGAGATGCTATATTAGGAGGAGTGAATCATAATGAGCTGAGTGTAGCTGGGACTGTAAGTTACCGGCCTTCAGATGGATTGACGGATTTGAAACTGGTAATCAAATTTCTGTCTTCTCTCTGTAAATAATGTTTTGAATTAGTTTAGTTGCCACTTGCCTGTATGAATTCATTTTTGACATGGAGAGATTTAATTGCTTGACCAGCTCTGTTTTTATTTTCTTGCATATGCTTGGATTTGTGTTCTCTAACATAGAATAAGGACATCAAATTTCTAAGCTGTTTCAAATGCTGATGACTTTATTTGAATATGTGCTTTCACCCTGTTAAAACCTTCTTCCTCTATTTATTTACATTCTTGATAATTATTCTTAGTTTGATTTATGAATATAGCTTTTGGAGACTGGAGAAACTCTATTTTGGCTGTGTTTAGAGATTAAAGGTGGTCCGGTCAATGTAAACGTAAAATTCCATTGAAGCATCCTTGGACCACCATTGTTAAGCTTATACTGAATGAAGTGAGATTTCAATATATGAAGCACAGTTGACTACAATGAGTATACCGGCTAACATGGTGTATTGTTGCAATTGAGTACCACCTTTGAGCTGGGACCTTTCATTTCCTATCAAGGAAATTATAATCATTTTCGAACGATTCCTCAATGTTAATGTTTGATTCTGATATTTATATCTCCTGAAAGTATTCTCCTTTCTGTATTTCTATCTTTATTGCCTAGCTTTTCTTTGTTAACATGTTAGAGAAGATGTTTAAACTTTCTCCATAATTTGTAGGTATTAGCTTTTTTATTACTTATTACTTATTACTTATTTCCTAGCTCTAGCTTGTCAGCTGTGTTGCCTCTAGCATTACCTTTTTACTTGTACTTCATTCGATTGGTAAGTAGACTACCATATGGACTTCCTTGTAATGGGTGTACTTTTGCCCAAAAGATTTACCTTGTCAATCATCTTCTCACAGGCTGTAGCAAGCTTCATGTCTCAAGTTGTAGGGAACTCTGTTTCATTCATCCCTTCCCATTTAGTATTTACGACCGGAGTGACCTCTGCAATCGAAACTCTTTGCTTTTGCATAGCAGACCCGGGAAATGCGTTTCTTGTTCCTACGCCGTATTACCCTGGGTACTTGTTCATTCTTCTTTCATGAAATACTACTTTAGTTTTATCTGGAAAAACTAAGAAACTAAATCATAAATGCAGTCTTGACAATGACGTGAAATGGCGAACTGGGGTGGAGATAGTACCCGTTCCCTGCCGCAGTGCCGATAACTTCAGTTTAAGCATAACTTCTCTTGATCGAGCATTCCATCAGGCAAGGAATCGCGGCCTTAAAGTTCGTGGAATTATAATCTCAAATCCCTCAATTCCGGTTGGAAATCAACTAAATAGAGAGACACTCTATGACCTTCTGGACTTTGCCAGGGAGAAAAACATCCATATCATATCAAATGAAATATTTGTAGGGTCGACCTACGGAAGTGAAGAGTTTGTGAGCATGGCTGAAATAATCGATGCAGAAGACAGTGACCGAGACAGAGTTCATATAACATATGCTCTATCTGAAGACATGTCACTTCCTGGTTTTGGAGTTGCTGCGATCTACTCTTTGAATGAGAATATCCTGGCTGCTGCTAGGAAACTGGCAAGGTTCTCATCTGTTTCCGCTATAACCCAGAACCTCCTTTCTTCTATGCTTTCAGATACGCAATTCATCCAGAGGTTCATTCGAACCAATCGAGAGAGGCTTGGAGAAATGGACGGTAAATTCACTGCAGGGCTGAAGGAACTCAACATCAAATGCATGAGGAGCAATGGAGGTTTCTACTGCTGGGCAGATATGAGCAAGTTAATCCGCTCTTACAGCGAGAAAGGGGAACTTGAGCTCTGGGAAAAGTTGGTGGATATAGGTAAAGTTAATGTTATCCCTGGCTCATCTTGTAATTGTATTGAACCGGGATGGTTTGGATTCTGTTTCACAACTTTGATTGATAAAGAAATACCTATTGTTATGGACCGAATTCGGAGAATTTCCGAAGGTTGTAAATCTTATACTTAAAATGATATTATTGTTCTGTACATATGTATCAATTGTATTAAACTTTTTCTAGGCCTTTGTAGTTGTTTATAGGCGACTTGTAAAGTGGTAGTACGGGATTTTGATCAATTGCTGCTACATTCTTAGCTTCTATAATTTTCTAGAGTAGAAATGGATTGCTACATTCTTAGCTTCTATAATTTTCTAGAGTAGAAATGGATGTTTCTGAACCTTCTTCAGTTGGAACCATTTTGTGGACTAAATTTAAGTCTTTCTTCTCACATAGTGAAACCAAAGTGAAGTAACTTCAGCTCCTGACTCGCAATTCGCCCCAAGTAGAGAATTCTCACCCTCATGCAGTACTCCATGTGACACAATTTTGTTGAACAAGTATAGACATCTTTAATCCATGAAATTATTTGTACCGTTAAATCGCTGAATTTACTTCGGTGCAGACGTATATTTAGTGATTTAACATTTCAATGAATTTCATGGATTAAAGACAGTCTATACTTTGATCAACAAAATTTAATATGAAGTTCTGTTTTCCTTTCCAAGGGACTAAAGCAATGAAACAAGCAAAACTAGCCGCTTTAATGGCTATACGCCTGTTTGAATGGTAATCATCCTCTCCTTTTTCTTGAAAGTGATACGCAAGGTCTATGAATCTTTCCAATCTTTTCTTCATTTTTCTATACAAGCATAGTCTCACAATCTCTCATTTTCGTTGTAGATTCGGTTCATTCATACGCCTCTCACCTAAAAGAGAAACTATTAGGAATCCTCTTTTTTGTGTGTGCTTCCTCTTTTGCACTAGCAATTGGTCCCGCTCCTCATTAGCTCAAGATAATAAATCGTACAAGTTCAAATTTCTACACTGTCATTATTCCACGTGAATAGTCCTAACCATGTTGGCAGGATCTTTTCTGAGTTTCTTCTTGCAACAGAACATATTATGAACAAACTTCAATAACTACAAAAGAAATTATCTGATTGATCCTATATCATATTTGGTCCCATACTTTGTGTTCATATGATCTAATTACTTCTTAAGCTTCACGAAGTTCAACAACTACTGGTCTTGAAATAGAGAAAATGTTGAAAAAAGATTGTCAACTCCCAACCTCTTACTACTATATAAGTTACACTCTAGTTTTCTTCAGGTTATATTTTAGTACTCTAGATAAATGACACATATATATTGAATAAAGAGTTCAATACATCTAGAAATCACTCAGAACTTCTCACCCTCACTAGAGAGACATAAAACCATGCCACCCTCTGATGATTCCCGTCACTTTCTCTGTTCCAAATGTGGGAATTTTTTCTGAAACTCAGTCATTAAAGCCAAATTCTCTTGATGAAGGATATTGGCTTTCTCTCTAAGCTTCTCATTCTCTTCAATTATGGTCTGATTTTCCAGATATAGCTGCAGATTCTTCAGCTCAATATCCTTCCCCAGCTTCTCTTCACATCTCCTCCTGCTAAAACAAAACAAAAACAAAAACAAAAACCCATAAACAAAGTTCCCTAAAACCCATAAAAAAGGTGAAATGGGGTTGTGTAAGAATTAGAAAGCAACCTTGTGAGTCTCAAAACTTTAAGTTTGGATCTCTTTAAACTTCTGTTCTTGAAACTCTGAATTGTATGAAACAGAACACTCTGTGTTGCACTGATGCACATTATTTTTCTATGGCAGCTTCTTAACTTTTGCCTTTCTTTCTAGTAGAATATTGTATGGAAG

mRNA sequence

CGTAAAATAAGAAACCCTTAAAAAAAAAAAAAAGAAAAAAGAAAAAAAAGGAAAAAAAATCGGGGTGAATTCATCTCCGCCTTAAAGCCAGAAGAATCGGCGCGGAAAGTACCAATGTAACATAAACATCATAAATTCTTTTATTTTTTTAAAATTCTTTTCGAAGGAAAAATGTTCAAATAATTCAATTTTGTCAATCCTACCCTTCGAGCTTTTTGCTTGCCCTGCCGGTCGCCGGAATTTTCCTCGTCTATCCGGTGAGAGAAGCTCCTCCACCCTCGCCCCTCGCCGGCGGCGGTTTATCTTCGGGTTCTGGTGGATTGAAATGACTCTGCAATGACCCAGACCCGATTCTTCAGAACTCGGAACTCCAAATCTGACAAAGAAGCAGAACCCACCACAAGCGGTGGCGGAGGAGCCGCCATGAGAGTCATAGTGCCTCTGCAAGGCGTCGTTCAAGGAAGAGGCGGCGTCGTTTTGGGCTCTGTTATACCTTGCGTTCTCTTCTACTTTCTTCAGCTCTACTTGAAACGAAACCGGTCCGAACCATCCCCGGACCCTCCTTCCTCGCAGGAGGTGGATTCTTCGGGGAACTCTTCGCCATCGTCGAGTAATCAGTTAGTGGAGCTCTCTGTACTGCCTCGAACATTGTCTCGGACCTTCCTCTCGCCGAGGTCGAGTGGGCCGCTTTGCGTTTCGGGTCGGGCAAATTCGATTTCTAGAGTTGGGGATTCTACGATTTTCGTTGGAATGAGGAGGTTCTTGGAGGACCCATTTGATGAATTGGATAATCCGACGGGGATTATTCAGCTTTCTGTTGCTGAGAATAAGTTATCCTCGGACTTGGTGCAAAATTGGCTAACGGAGAATGGAAGAGATGCTATATTAGGAGGAGTGAATCATAATGAGCTGAGTGTAGCTGGGACTGTAAGTTACCGGCCTTCAGATGGATTGACGGATTTGAAACTGGCTGTAGCAAGCTTCATGTCTCAAGTTGTAGGGAACTCTGTTTCATTCATCCCTTCCCATTTAGTATTTACGACCGGAGTGACCTCTGCAATCGAAACTCTTTGCTTTTGCATAGCAGACCCGGGAAATGCGTTTCTTGTTCCTACGCCGTATTACCCTGGTCTTGACAATGACGTGAAATGGCGAACTGGGGTGGAGATAGTACCCGTTCCCTGCCGCAGTGCCGATAACTTCAGTTTAAGCATAACTTCTCTTGATCGAGCATTCCATCAGGCAAGGAATCGCGGCCTTAAAGTTCGTGGAATTATAATCTCAAATCCCTCAATTCCGGTTGGAAATCAACTAAATAGAGAGACACTCTATGACCTTCTGGACTTTGCCAGGGAGAAAAACATCCATATCATATCAAATGAAATATTTGTAGGGTCGACCTACGGAAGTGAAGAGTTTGTGAGCATGGCTGAAATAATCGATGCAGAAGACAGTGACCGAGACAGAGTTCATATAACATATGCTCTATCTGAAGACATGTCACTTCCTGGTTTTGGAGTTGCTGCGATCTACTCTTTGAATGAGAATATCCTGGCTGCTGCTAGGAAACTGGCAAGGTTCTCATCTGTTTCCGCTATAACCCAGAACCTCCTTTCTTCTATGCTTTCAGATACGCAATTCATCCAGAGGTTCATTCGAACCAATCGAGAGAGGCTTGGAGAAATGGACGGTAAATTCACTGCAGGGCTGAAGGAACTCAACATCAAATGCATGAGGAGCAATGGAGGTTTCTACTGCTGGGCAGATATGAGCAAGTTAATCCGCTCTTACAGCGAGAAAGGGGAACTTGAGCTCTGGGAAAAGTTGGTGGATATAGGTAAAGTTAATGTTATCCCTGGCTCATCTTGTAATTGTATTGAACCGGGATGGTTTGGATTCTGTTTCACAACTTTGATTGATAAAGAAATACCTATTGTTATGGACCGAATTCGGAGAATTTCCGAAGGTTGTAAATCTTATACTTAAAATGATATTATTGTTCTGTACATATGTATCAATTGTATTAAACTTTTTCTAGGCCTTTGTAGTTGTTTATAGGCGACTTGTAAAGTGGTAGTACGGGATTTTGATCAATTGCTGCTACATTCTTAGCTTCTATAATTTTCTAGAGTAGAAATGGATTGCTACATTCTTAGCTTCTATAATTTTCTAGAGTAGAAATGGATGTTTCTGAACCTTCTTCAGTTGGAACCATTTTGTGGACTAAATTTAAGTCTTTCTTCTCACATAGTGAAACCAAAGTGAAGTAACTTCAGCTCCTGACTCGCAATTCGCCCCAAGTAGAGAATTCTCACCCTCATGCAGTACTCCATGTGACACAATTTTGTTGAACAAGTATAGACATCTTTAATCCATGAAATTATTTGTACCGTTAAATCGCTGAATTTACTTCGGTGCAGACGTATATTTAGTGATTTAACATTTCAATGAATTTCATGGATTAAAGACAGTCTATACTTTGATCAACAAAATTTAATATGAAGTTCTGTTTTCCTTTCCAAGGGACTAAAGCAATGAAACAAGCAAAACTAGCCGCTTTAATGGCTATACGCCTGTTTGAATGGTAATCATCCTCTCCTTTTTCTTGAAAGTGATACGCAAGGTCTATGAATCTTTCCAATCTTTTCTTCATTTTTCTATACAAGCATAGTCTCACAATCTCTCATTTTCGTTGTAGATTCGGTTCATTCATACGCCTCTCACCTAAAAGAGAAACTATTAGGAATCCTCTTTTTTGTGTGTGCTTCCTCTTTTGCACTAGCAATTGGTCCCGCTCCTCATTAGCTCAAGATAATAAATCGTACAAGTTCAAATTTCTACACTGTCATTATTCCACGTGAATAGTCCTAACCATGTTGGCAGGATCTTTTCTGAGTTTCTTCTTGCAACAGAACATATTATGAACAAACTTCAATAACTACAAAAGAAATTATCTGATTGATCCTATATCATATTTGGTCCCATACTTTGTGTTCATATGATCTAATTACTTCTTAAGCTTCACGAAGTTCAACAACTACTGGTCTTGAAATAGAGAAAATGTTGAAAAAAGATTGTCAACTCCCAACCTCTTACTACTATATAAGTTACACTCTAGTTTTCTTCAGGTTATATTTTAGTACTCTAGATAAATGACACATATATATTGAATAAAGAGTTCAATACATCTAGAAATCACTCAGAACTTCTCACCCTCACTAGAGAGACATAAAACCATGCCACCCTCTGATGATTCCCGTCACTTTCTCTGTTCCAAATGTGGGAATTTTTTCTGAAACTCAGTCATTAAAGCCAAATTCTCTTGATGAAGGATATTGGCTTTCTCTCTAAGCTTCTCATTCTCTTCAATTATGGTCTGATTTTCCAGATATAGCTGCAGATTCTTCAGCTCAATATCCTTCCCCAGCTTCTCTTCACATCTCCTCCTGCTAAAACAAAACAAAAACAAAAACAAAAACCCATAAACAAAGTTCCCTAAAACCCATAAAAAAGGTGAAATGGGGTTGTGTAAGAATTAGAAAGCAACCTTGTGAGTCTCAAAACTTTAAGTTTGGATCTCTTTAAACTTCTGTTCTTGAAACTCTGAATTGTATGAAACAGAACACTCTGTGTTGCACTGATGCACATTATTTTTCTATGGCAGCTTCTTAACTTTTGCCTTTCTTTCTAGTAGAATATTGTATGGAAG

Coding sequence (CDS)

ATGACCCAGACCCGATTCTTCAGAACTCGGAACTCCAAATCTGACAAAGAAGCAGAACCCACCACAAGCGGTGGCGGAGGAGCCGCCATGAGAGTCATAGTGCCTCTGCAAGGCGTCGTTCAAGGAAGAGGCGGCGTCGTTTTGGGCTCTGTTATACCTTGCGTTCTCTTCTACTTTCTTCAGCTCTACTTGAAACGAAACCGGTCCGAACCATCCCCGGACCCTCCTTCCTCGCAGGAGGTGGATTCTTCGGGGAACTCTTCGCCATCGTCGAGTAATCAGTTAGTGGAGCTCTCTGTACTGCCTCGAACATTGTCTCGGACCTTCCTCTCGCCGAGGTCGAGTGGGCCGCTTTGCGTTTCGGGTCGGGCAAATTCGATTTCTAGAGTTGGGGATTCTACGATTTTCGTTGGAATGAGGAGGTTCTTGGAGGACCCATTTGATGAATTGGATAATCCGACGGGGATTATTCAGCTTTCTGTTGCTGAGAATAAGTTATCCTCGGACTTGGTGCAAAATTGGCTAACGGAGAATGGAAGAGATGCTATATTAGGAGGAGTGAATCATAATGAGCTGAGTGTAGCTGGGACTGTAAGTTACCGGCCTTCAGATGGATTGACGGATTTGAAACTGGCTGTAGCAAGCTTCATGTCTCAAGTTGTAGGGAACTCTGTTTCATTCATCCCTTCCCATTTAGTATTTACGACCGGAGTGACCTCTGCAATCGAAACTCTTTGCTTTTGCATAGCAGACCCGGGAAATGCGTTTCTTGTTCCTACGCCGTATTACCCTGGTCTTGACAATGACGTGAAATGGCGAACTGGGGTGGAGATAGTACCCGTTCCCTGCCGCAGTGCCGATAACTTCAGTTTAAGCATAACTTCTCTTGATCGAGCATTCCATCAGGCAAGGAATCGCGGCCTTAAAGTTCGTGGAATTATAATCTCAAATCCCTCAATTCCGGTTGGAAATCAACTAAATAGAGAGACACTCTATGACCTTCTGGACTTTGCCAGGGAGAAAAACATCCATATCATATCAAATGAAATATTTGTAGGGTCGACCTACGGAAGTGAAGAGTTTGTGAGCATGGCTGAAATAATCGATGCAGAAGACAGTGACCGAGACAGAGTTCATATAACATATGCTCTATCTGAAGACATGTCACTTCCTGGTTTTGGAGTTGCTGCGATCTACTCTTTGAATGAGAATATCCTGGCTGCTGCTAGGAAACTGGCAAGGTTCTCATCTGTTTCCGCTATAACCCAGAACCTCCTTTCTTCTATGCTTTCAGATACGCAATTCATCCAGAGGTTCATTCGAACCAATCGAGAGAGGCTTGGAGAAATGGACGGTAAATTCACTGCAGGGCTGAAGGAACTCAACATCAAATGCATGAGGAGCAATGGAGGTTTCTACTGCTGGGCAGATATGAGCAAGTTAATCCGCTCTTACAGCGAGAAAGGGGAACTTGAGCTCTGGGAAAAGTTGGTGGATATAGGTAAAGTTAATGTTATCCCTGGCTCATCTTGTAATTGTATTGAACCGGGATGGTTTGGATTCTGTTTCACAACTTTGATTGATAAAGAAATACCTATTGTTATGGACCGAATTCGGAGAATTTCCGAAGGTTGTAAATCTTATACTTAA

Protein sequence

MTQTRFFRTRNSKSDKEAEPTTSGGGGAAMRVIVPLQGVVQGRGGVVLGSVIPCVLFYFLQLYLKRNRSEPSPDPPSSQEVDSSGNSSPSSSNQLVELSVLPRTLSRTFLSPRSSGPLCVSGRANSISRVGDSTIFVGMRRFLEDPFDELDNPTGIIQLSVAENKLSSDLVQNWLTENGRDAILGGVNHNELSVAGTVSYRPSDGLTDLKLAVASFMSQVVGNSVSFIPSHLVFTTGVTSAIETLCFCIADPGNAFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSITSLDRAFHQARNRGLKVRGIIISNPSIPVGNQLNRETLYDLLDFAREKNIHIISNEIFVGSTYGSEEFVSMAEIIDAEDSDRDRVHITYALSEDMSLPGFGVAAIYSLNENILAAARKLARFSSVSAITQNLLSSMLSDTQFIQRFIRTNRERLGEMDGKFTAGLKELNIKCMRSNGGFYCWADMSKLIRSYSEKGELELWEKLVDIGKVNVIPGSSCNCIEPGWFGFCFTTLIDKEIPIVMDRIRRISEGCKSYT
Homology
BLAST of Bhi12G000329 vs. TAIR 10
Match: AT1G62960.1 (ACC synthase 10 )

HSP 1 Score: 625.5 bits (1612), Expect = 3.9e-179
Identity = 328/563 (58.26%), Postives = 416/563 (73.89%), Query Frame = 0

Query: 1   MTQTRFFRTRNSKSDKEAEPTTSGGG-GAAMRVIVPLQGVVQGRGGVVLGSVIPCVLFYF 60
           MT+T   R+R+S SD +      GGG    MRVIVPLQGVVQGRGG+ LGSVIPC  FYF
Sbjct: 1   MTRTEPNRSRSSNSDSDKNSGNVGGGRTTGMRVIVPLQGVVQGRGGLFLGSVIPCAFFYF 60

Query: 61  LQLYLKRNRSEPSPDPPSSQEVDSSGNSSPS----------SSNQLVELSVLPRTLSRTF 120
           LQ YLKRNR     D    Q   +S +SSP+          S+  L EL+ LPR+LSR  
Sbjct: 61  LQFYLKRNRKNDESDNSGEQNSSASSSSSPNSGLPDPTRSQSAGHLTELTGLPRSLSRIL 120

Query: 121 LSPRSS-GPLCVSGRANSISRVGDST-IFVGMRRFLEDPFDELDNPTGIIQLSVAE-NKL 180
           LSPR+S G + VSGR N + + GDS+  +VG +R  +DP+DEL NP G+IQL +A+ NKL
Sbjct: 121 LSPRNSGGAVSVSGRVNCVLKGGDSSPYYVGQKRVEDDPYDELGNPDGVIQLGLAQNNKL 180

Query: 181 SSDLVQNWLTENGRDAILGGVNHNELSVAGTVSYRPSDGLTDLKLAVASFMSQVVGNSVS 240
           S D   +W+ EN ++AI  G     LS++G  SY PSDGL +LK+AVA FM++   NSV+
Sbjct: 181 SLD---DWVLENPKEAISDG-----LSISGIASYEPSDGLLELKMAVAGFMTEATKNSVT 240

Query: 241 FIPSHLVFTTGVTSAIETLCFCIADPGNAFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSA 300
           F PS LV T+G +SAIE L FC+AD GNAFLVPTP  PG D DVKWRTGV+I+ VPCRSA
Sbjct: 241 FDPSQLVLTSGASSAIEILSFCLADSGNAFLVPTPCSPGYDRDVKWRTGVDIIHVPCRSA 300

Query: 301 DNFSLSITSLDRAFHQARNRGLKVRGIIISNPSIPVGNQLNRETLYDLLDFAREKNIHII 360
           DNF++S+  LDRAF+QA+ RG+++RGIIISNPS P+G+ L+RE LY LLDFARE+NIHII
Sbjct: 301 DNFNMSMVVLDRAFYQAKKRGVRIRGIIISNPSNPMGSLLSRENLYALLDFARERNIHII 360

Query: 361 SNEIFVGSTYGSE-EFVSMAEIIDAEDS-DRDRVHITYALSEDMSLPGFGVAAIYSLNEN 420
           SNEIF GS +G E EFVSMAEI+D E++ DR+RVHI Y LS+D+S  G   AAIYS NE+
Sbjct: 361 SNEIFAGSVHGEEGEFVSMAEIVDTEENIDRERVHIVYDLSKDLSFRGLRSAAIYSFNES 420

Query: 421 ILAAARKLARFSSVSAITQNLLSSMLSDTQFIQRFIRTNRERLGEMDGKFTAGLKELNIK 480
           +L+A+RKL   S VS+ TQ+LL S +S+ + +QRF++TNR+RL  +  +   GLKEL I+
Sbjct: 421 VLSASRKLTTLSPVSSPTQHLLISAISNPKNVQRFVKTNRQRLQSIYTELVEGLKELGIE 480

Query: 481 CMRSNGGFYCWADMSKLIRSYSEKGELELWEKLVDIGKVNVIPGSSCNCIEPGWFGFCFT 540
           C RSNGGFYCWADM  LI SYSEKGE+ELW KL++IGK+NVIPGS C+CIEPGWF  CF+
Sbjct: 481 CTRSNGGFYCWADMRGLISSYSEKGEIELWNKLLNIGKINVIPGSCCHCIEPGWFRICFS 540

Query: 541 TLIDKEIPIVMDRIRRISEGCKS 548
            L ++++P+VM+RIR++ E CKS
Sbjct: 541 NLSERDVPVVMNRIRKVCETCKS 555

BLAST of Bhi12G000329 vs. TAIR 10
Match: AT5G51690.1 (1-amino-cyclopropane-1-carboxylate synthase 12 )

HSP 1 Score: 550.8 bits (1418), Expect = 1.2e-156
Identity = 269/520 (51.73%), Postives = 370/520 (71.15%), Query Frame = 0

Query: 30  MRVIVPLQGVVQGRGGVVLGSVIPCVLFYFLQLYLKRNRSEPSPDPPSSQEVDSSGNSSP 89
           MR+IVPL+GV+QGRGG+ +GS+IPC LFYFLQLYLKR R      PP S   D       
Sbjct: 1   MRLIVPLRGVIQGRGGLFVGSLIPCCLFYFLQLYLKRRR------PPPSDPTD------- 60

Query: 90  SSSNQLVELSVLPRTLSRTFLSPR--SSGPLCVSGRANSISRVGDSTIFVGMRRFLEDPF 149
                      LPRT SRT L  R  S G + VS RA  +++  DS  ++G+ R   DP+
Sbjct: 61  -----------LPRTFSRTNLFSRGNSIGRVRVSSRAVPVAKPSDSPYYIGLERVKTDPY 120

Query: 150 DELDNPTGIIQLSVAENKLSSDLVQNWLTENGRDAILGGVNHNELSVAGTVSYRPSDGLT 209
           D + N  GIIQL +AE+ L  DL+Q W++EN  ++++   +  E  ++    Y+P +GL 
Sbjct: 121 DRITNTDGIIQLGLAESTLCFDLLQRWMSENLMESMMQS-DDGEFDISSIAMYKPFEGLL 180

Query: 210 DLKLAVASFMSQVVGNSVSFIPSHLVFTTGVTSAIETLCFCIADPGNAFLVPTPYYPGLD 269
           +L++A A FMS+++G +VSF PS++V T G T AIE L FC+AD GNAFL+PTPYYPG D
Sbjct: 181 ELRVAFADFMSRIMGGNVSFDPSNMVITAGGTPAIEVLAFCLADHGNAFLIPTPYYPGFD 240

Query: 270 NDVKWRTGVEIVPVPCRSADNFSLSITSLDRAFHQARNRGLKVRGIIISNPSIPVGNQLN 329
            D+K+RTGVE++PV CRS+DNF++++++L++A +QAR RG KV GI+ SNPS PVGN L+
Sbjct: 241 RDIKFRTGVELIPVHCRSSDNFTVTVSALEQALNQARKRGSKVSGILFSNPSNPVGNILS 300

Query: 330 RETLYDLLDFAREKNIHIISNEIFVGSTYGSEEFVSMAEIIDAEDSDRDRVHITYALSED 389
           RETL D+L FA+EKNIH+IS+EIF GS YG +EFVSMAEI  + + D+ RVHI Y LS+D
Sbjct: 301 RETLCDILRFAQEKNIHVISDEIFAGSVYGDKEFVSMAEIAGSGEFDKTRVHIIYGLSKD 360

Query: 390 MSLPGFGVAAIYSLNENILAAARKLARFSSVSAITQNLLSSMLSDTQFIQRFIRTNRERL 449
           +S+PGF    IYS +E+++ AA+KL RFSSV  + Q +L S+LSD +FI+ ++  +R+R+
Sbjct: 361 LSIPGFRAGVIYSFHEDVVNAAKKLMRFSSVPVLVQRILISLLSDVRFIEGYMAAHRQRI 420

Query: 450 GEMDGKFTAGLKELNIKCMRSNGGFYCWADMSKLIRSYSEKGELELWEKLVDIGKVNVIP 509
            +   +F  GLK+L I C  S GG YCW DMS L+ SYSEKGELEL+EKL+ + K+N  P
Sbjct: 421 RDKHIRFVEGLKQLGIPCAESGGGLYCWVDMSSLLTSYSEKGELELFEKLLTVAKINATP 480

Query: 510 GSSCNCIEPGWFGFCFTTLIDKEIPIVMDRIRRISEGCKS 548
           G++C CIEPGWF  CFT L D++IP++M+RIR+++E  +S
Sbjct: 481 GTACYCIEPGWFRCCFTALADEDIPVIMERIRQLAESFRS 495

BLAST of Bhi12G000329 vs. TAIR 10
Match: AT4G37770.1 (1-amino-cyclopropane-1-carboxylate synthase 8 )

HSP 1 Score: 385.6 bits (989), Expect = 6.8e-107
Identity = 190/432 (43.98%), Postives = 286/432 (66.20%), Query Frame = 0

Query: 120 VSGRANSISRVGDSTIFVGMRRFLEDPFDELDNPTGIIQLSVAENKLSSDLVQNWLTENG 179
           +S +A+  +   DS+ F G   + ++P+DE+ NP GIIQ+ +AEN+LS DL+++WL +N 
Sbjct: 4   LSKKASCNTHGQDSSYFWGWEEYEKNPYDEIKNPDGIIQMGLAENQLSFDLIESWLAKNP 63

Query: 180 RDAIL---GGVNHNELSVAGTVSYRPSDGLTDLKLAVASFMSQVVGNSVSFIPSHLVFTT 239
             A     G     EL++     ++   GL   K A+A FMS+  GN VSF P+ LV T 
Sbjct: 64  DAANFQREGQSIFRELAL-----FQDYHGLPSFKNAMADFMSENRGNRVSFNPNKLVLTA 123

Query: 240 GVTSAIETLCFCIADPGNAFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSITSL 299
           G T A ETL FC+ADPG+AFL+PTPYYPG D D+KWRTG EIVP+ C+SA+ F ++  +L
Sbjct: 124 GATPANETLMFCLADPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIQCKSANGFRITKVAL 183

Query: 300 DRAFHQARNRGLKVRGIIISNPSIPVGNQLNRETLYDLLDFAREKNIHIISNEIFVGSTY 359
           + A+ QA+   LKV+G++I+NPS P+G    R  L  LLDF   K IH+IS+EI+ G+ +
Sbjct: 184 EEAYEQAQKLNLKVKGVLITNPSNPLGTTTTRTELNHLLDFISRKKIHLISDEIYSGTVF 243

Query: 360 GSEEFVSMAEII---DAEDSD-RDRVHITYALSEDMSLPGFGVAAIYSLNENILAAARKL 419
            +  F+S+ E++     E++D  DRVHI Y+LS+D+ LPGF V  IYS ++ +++AA K+
Sbjct: 244 TNPGFISVMEVLKDRKLENTDVFDRVHIVYSLSKDLGLPGFRVGVIYSNDDFVVSAATKM 303

Query: 420 ARFSSVSAITQNLLSSMLSDTQFIQRFIRTNRERLGEMDGKFTAGLKELNIKCMRSNGGF 479
           + F  +S+ TQ LLS++LSD  F + ++  N+ RL     K  +GL+   I+C++SN G 
Sbjct: 304 SSFGLISSQTQYLLSALLSDKTFTKNYLEENQIRLKNRHKKLVSGLEAAGIECLKSNAGL 363

Query: 480 YCWADMSKLIRSYSEKGELELWEKLVDIGKVNVIPGSSCNCIEPGWFGFCFTTLIDKEIP 539
           +CW DM  L++S + + E+ELW+K+V   K+N+ PGSSC+C EPGWF  CF  L ++ + 
Sbjct: 364 FCWVDMRHLLKSNTFEAEIELWKKIVYEVKLNISPGSSCHCNEPGWFRVCFANLSEETLK 423

Query: 540 IVMDRIRRISEG 545
           + +DR++R  +G
Sbjct: 424 VALDRLKRFVDG 430

BLAST of Bhi12G000329 vs. TAIR 10
Match: AT3G49700.1 (1-aminocyclopropane-1-carboxylate synthase 9 )

HSP 1 Score: 384.4 bits (986), Expect = 1.5e-106
Identity = 187/433 (43.19%), Postives = 288/433 (66.51%), Query Frame = 0

Query: 120 VSGRANSISRVGDSTIFVGMRRFLEDPFDELDNPTGIIQLSVAENKLSSDLVQNWLTENG 179
           +S +  S +   DS+ F+G   + ++P+DE+ NP GIIQ+ +AEN+L  DL++ WL +N 
Sbjct: 4   LSRKVTSNAHGQDSSYFLGWEEYEKNPYDEIKNPNGIIQMGLAENQLCFDLIETWLAKNP 63

Query: 180 RDAILGGVNHNELSVAGTVS-YRPSDGLTDLKLAVASFMSQVVGNSVSFIPSHLVFTTGV 239
             A   G+  +  S+   ++ ++   GL + K A+A FM ++ GN V+F PS +V   G 
Sbjct: 64  DAA---GLKKDGQSIFKELALFQDYHGLPEFKKALAEFMEEIRGNRVTFDPSKIVLAAGS 123

Query: 240 TSAIETLCFCIADPGNAFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSITSLDR 299
           TSA ETL FC+A+PG+AFL+PTPYYPG D D+KWRTG EIVP+ C S++ F ++ ++L +
Sbjct: 124 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFQITESALQQ 183

Query: 300 AFHQARNRGLKVRGIIISNPSIPVGNQLNRETLYDLLDFAREKNIHIISNEIFVGSTYGS 359
           A+ QA+   LKV+G++++NPS P+G  L R  L  L+DF   KNIH+IS+EI+ G+ +G 
Sbjct: 184 AYQQAQKLDLKVKGVLVTNPSNPLGTMLTRRELNLLVDFITSKNIHLISDEIYSGTVFGF 243

Query: 360 EEFVSMAEII---DAEDSD-RDRVHITYALSEDMSLPGFGVAAIYSLNENILAAARKLAR 419
           E+FVS+ +++   + E+S+   RVHI Y+LS+D+ LPGF V AIYS +E +++AA K++ 
Sbjct: 244 EQFVSVMDVLKDKNLENSEVSKRVHIVYSLSKDLGLPGFRVGAIYSNDEMVVSAATKMSS 303

Query: 420 FSSVSAITQNLLSSMLSDTQFIQRFIRTNRERLGEMDGKFTAGLKELNIKCMRSNGGFYC 479
           F  VS+ TQ LLS++LSD +F   ++  N++RL     K  +GL+   I C++SN G +C
Sbjct: 304 FGLVSSQTQYLLSALLSDKKFTSTYLDENQKRLKIRQKKLVSGLEAAGITCLKSNAGLFC 363

Query: 480 WADMSKLIRSYSEKGELELWEKLVDIGKVNVIPGSSCNCIEPGWFGFCFTTLIDKEIPIV 539
           W DM  L+ + + + ELELW+K+V   K+N+ PGSSC+C EPGWF  CF  + +  + + 
Sbjct: 364 WVDMRHLLDTNTFEAELELWKKIVYDVKLNISPGSSCHCTEPGWFRVCFANMSEDTLDLA 423

Query: 540 MDRIRRISEGCKS 548
           M R++   E   S
Sbjct: 424 MKRLKEYVESTDS 433

BLAST of Bhi12G000329 vs. TAIR 10
Match: AT5G65800.1 (ACC synthase 5 )

HSP 1 Score: 381.7 bits (979), Expect = 9.8e-106
Identity = 182/429 (42.42%), Postives = 286/429 (66.67%), Query Frame = 0

Query: 120 VSGRANSISRVGDSTIFVGMRRFLEDPFDELDNPTGIIQLSVAENKLSSDLVQNWLTENG 179
           +S +  S     DS+ F+G   + ++P+DE+ NP G+IQ+ +AEN+L  DL+++WLT+N 
Sbjct: 4   LSTKVTSNGHGQDSSYFLGWEEYEKNPYDEIKNPNGMIQMGLAENQLCFDLIESWLTKNP 63

Query: 180 RDAILGGVNHNELSVAGTVS-YRPSDGLTDLKLAVASFMSQVVGNSVSFIPSHLVFTTGV 239
             A L     N  S+   ++ ++   G+ + K A+A FM ++ GN V+F P  +V   G 
Sbjct: 64  DAASL---KRNGQSIFRELALFQDYHGMPEFKKAMAEFMEEIRGNRVTFDPKKIVLAAGS 123

Query: 240 TSAIETLCFCIADPGNAFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSITSLDR 299
           TSA ETL FC+A+PG+AFL+PTPYYPG D D+KWRTG EIVP+ C S++ F ++ ++L +
Sbjct: 124 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFQITESALQQ 183

Query: 300 AFHQARNRGLKVRGIIISNPSIPVGNQLNRETLYDLLDFAREKNIHIISNEIFVGSTYGS 359
           A+ QA+   LKV+G++++NPS P+G  L R  L  L+DF   KNIH+IS+EI+ G+ +G 
Sbjct: 184 AYQQAQKLDLKVKGVLVTNPSNPLGTALTRRELNLLVDFITSKNIHLISDEIYSGTMFGF 243

Query: 360 EEFVSMAEII---DAEDSD-RDRVHITYALSEDMSLPGFGVAAIYSLNENILAAARKLAR 419
           E+F+S+ +++     ED++   RVH+ Y+LS+D+ LPGF V AIYS +E I++AA K++ 
Sbjct: 244 EQFISVMDVLKDKKLEDTEVSKRVHVVYSLSKDLGLPGFRVGAIYSNDEMIVSAATKMSS 303

Query: 420 FSSVSAITQNLLSSMLSDTQFIQRFIRTNRERLGEMDGKFTAGLKELNIKCMRSNGGFYC 479
           F  VS+ TQ LLS++LSD +F  +++  N++RL     +  +GL+   I C+RSN G +C
Sbjct: 304 FGLVSSQTQYLLSALLSDKKFTSQYLEENQKRLKSRQRRLVSGLESAGITCLRSNAGLFC 363

Query: 480 WADMSKLIRSYSEKGELELWEKLVDIGKVNVIPGSSCNCIEPGWFGFCFTTLIDKEIPIV 539
           W DM  L+ + + + EL+LW+K+V   K+N+ PGSSC+C EPGWF  CF  + +  + + 
Sbjct: 364 WVDMRHLLDTNTFEAELDLWKKIVYNVKLNISPGSSCHCTEPGWFRVCFANMSEDTLDLA 423

Query: 540 MDRIRRISE 544
           + R++   E
Sbjct: 424 LKRLKTFVE 429

BLAST of Bhi12G000329 vs. ExPASy Swiss-Prot
Match: Q9LQ10 (Probable aminotransferase ACS10 OS=Arabidopsis thaliana OX=3702 GN=ACS10 PE=1 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 5.5e-178
Identity = 328/563 (58.26%), Postives = 416/563 (73.89%), Query Frame = 0

Query: 1   MTQTRFFRTRNSKSDKEAEPTTSGGG-GAAMRVIVPLQGVVQGRGGVVLGSVIPCVLFYF 60
           MT+T   R+R+S SD +      GGG    MRVIVPLQGVVQGRGG+ LGSVIPC  FYF
Sbjct: 1   MTRTEPNRSRSSNSDSDKNSGNVGGGRTTGMRVIVPLQGVVQGRGGLFLGSVIPCAFFYF 60

Query: 61  LQLYLKRNRSEPSPDPPSSQEVDSSGNSSPS----------SSNQLVELSVLPRTLSRTF 120
           LQ YLKRNR     D    Q   +S +SSP+          S+  L EL+ LPR+LSR  
Sbjct: 61  LQFYLKRNRKNDESDNSGEQNSSASSSSSPNSGLPDPTRSQSAGHLTELTGLPRSLSRIL 120

Query: 121 LSPRSS-GPLCVSGRANSISRVGDST-IFVGMRRFLEDPFDELDNPTGIIQLSVAE-NKL 180
           LSPR+S G + VSGR N + + GDS+  +VG +R  +DP+DEL NP G+IQL +A+ NKL
Sbjct: 121 LSPRNSGGAVSVSGRVNCVLKGGDSSPYYVGQKRVEDDPYDELGNPDGVIQLGLAQNNKL 180

Query: 181 SSDLVQNWLTENGRDAILGGVNHNELSVAGTVSYRPSDGLTDLKLAVASFMSQVVGNSVS 240
           S D   +W+ EN ++AI  G     LS++G  SY PSDGL +LK+AVA FM++   NSV+
Sbjct: 181 SLD---DWVLENPKEAISDG-----LSISGIASYEPSDGLLELKMAVAGFMTEATKNSVT 240

Query: 241 FIPSHLVFTTGVTSAIETLCFCIADPGNAFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSA 300
           F PS LV T+G +SAIE L FC+AD GNAFLVPTP  PG D DVKWRTGV+I+ VPCRSA
Sbjct: 241 FDPSQLVLTSGASSAIEILSFCLADSGNAFLVPTPCSPGYDRDVKWRTGVDIIHVPCRSA 300

Query: 301 DNFSLSITSLDRAFHQARNRGLKVRGIIISNPSIPVGNQLNRETLYDLLDFAREKNIHII 360
           DNF++S+  LDRAF+QA+ RG+++RGIIISNPS P+G+ L+RE LY LLDFARE+NIHII
Sbjct: 301 DNFNMSMVVLDRAFYQAKKRGVRIRGIIISNPSNPMGSLLSRENLYALLDFARERNIHII 360

Query: 361 SNEIFVGSTYGSE-EFVSMAEIIDAEDS-DRDRVHITYALSEDMSLPGFGVAAIYSLNEN 420
           SNEIF GS +G E EFVSMAEI+D E++ DR+RVHI Y LS+D+S  G   AAIYS NE+
Sbjct: 361 SNEIFAGSVHGEEGEFVSMAEIVDTEENIDRERVHIVYDLSKDLSFRGLRSAAIYSFNES 420

Query: 421 ILAAARKLARFSSVSAITQNLLSSMLSDTQFIQRFIRTNRERLGEMDGKFTAGLKELNIK 480
           +L+A+RKL   S VS+ TQ+LL S +S+ + +QRF++TNR+RL  +  +   GLKEL I+
Sbjct: 421 VLSASRKLTTLSPVSSPTQHLLISAISNPKNVQRFVKTNRQRLQSIYTELVEGLKELGIE 480

Query: 481 CMRSNGGFYCWADMSKLIRSYSEKGELELWEKLVDIGKVNVIPGSSCNCIEPGWFGFCFT 540
           C RSNGGFYCWADM  LI SYSEKGE+ELW KL++IGK+NVIPGS C+CIEPGWF  CF+
Sbjct: 481 CTRSNGGFYCWADMRGLISSYSEKGEIELWNKLLNIGKINVIPGSCCHCIEPGWFRICFS 540

Query: 541 TLIDKEIPIVMDRIRRISEGCKS 548
            L ++++P+VM+RIR++ E CKS
Sbjct: 541 NLSERDVPVVMNRIRKVCETCKS 555

BLAST of Bhi12G000329 vs. ExPASy Swiss-Prot
Match: Q8GYY0 (Probable aminotransferase ACS12 OS=Arabidopsis thaliana OX=3702 GN=ACS12 PE=2 SV=2)

HSP 1 Score: 550.8 bits (1418), Expect = 1.7e-155
Identity = 269/520 (51.73%), Postives = 370/520 (71.15%), Query Frame = 0

Query: 30  MRVIVPLQGVVQGRGGVVLGSVIPCVLFYFLQLYLKRNRSEPSPDPPSSQEVDSSGNSSP 89
           MR+IVPL+GV+QGRGG+ +GS+IPC LFYFLQLYLKR R      PP S   D       
Sbjct: 1   MRLIVPLRGVIQGRGGLFVGSLIPCCLFYFLQLYLKRRR------PPPSDPTD------- 60

Query: 90  SSSNQLVELSVLPRTLSRTFLSPR--SSGPLCVSGRANSISRVGDSTIFVGMRRFLEDPF 149
                      LPRT SRT L  R  S G + VS RA  +++  DS  ++G+ R   DP+
Sbjct: 61  -----------LPRTFSRTNLFSRGNSIGRVRVSSRAVPVAKPSDSPYYIGLERVKTDPY 120

Query: 150 DELDNPTGIIQLSVAENKLSSDLVQNWLTENGRDAILGGVNHNELSVAGTVSYRPSDGLT 209
           D + N  GIIQL +AE+ L  DL+Q W++EN  ++++   +  E  ++    Y+P +GL 
Sbjct: 121 DRITNTDGIIQLGLAESTLCFDLLQRWMSENLMESMMQS-DDGEFDISSIAMYKPFEGLL 180

Query: 210 DLKLAVASFMSQVVGNSVSFIPSHLVFTTGVTSAIETLCFCIADPGNAFLVPTPYYPGLD 269
           +L++A A FMS+++G +VSF PS++V T G T AIE L FC+AD GNAFL+PTPYYPG D
Sbjct: 181 ELRVAFADFMSRIMGGNVSFDPSNMVITAGGTPAIEVLAFCLADHGNAFLIPTPYYPGFD 240

Query: 270 NDVKWRTGVEIVPVPCRSADNFSLSITSLDRAFHQARNRGLKVRGIIISNPSIPVGNQLN 329
            D+K+RTGVE++PV CRS+DNF++++++L++A +QAR RG KV GI+ SNPS PVGN L+
Sbjct: 241 RDIKFRTGVELIPVHCRSSDNFTVTVSALEQALNQARKRGSKVSGILFSNPSNPVGNILS 300

Query: 330 RETLYDLLDFAREKNIHIISNEIFVGSTYGSEEFVSMAEIIDAEDSDRDRVHITYALSED 389
           RETL D+L FA+EKNIH+IS+EIF GS YG +EFVSMAEI  + + D+ RVHI Y LS+D
Sbjct: 301 RETLCDILRFAQEKNIHVISDEIFAGSVYGDKEFVSMAEIAGSGEFDKTRVHIIYGLSKD 360

Query: 390 MSLPGFGVAAIYSLNENILAAARKLARFSSVSAITQNLLSSMLSDTQFIQRFIRTNRERL 449
           +S+PGF    IYS +E+++ AA+KL RFSSV  + Q +L S+LSD +FI+ ++  +R+R+
Sbjct: 361 LSIPGFRAGVIYSFHEDVVNAAKKLMRFSSVPVLVQRILISLLSDVRFIEGYMAAHRQRI 420

Query: 450 GEMDGKFTAGLKELNIKCMRSNGGFYCWADMSKLIRSYSEKGELELWEKLVDIGKVNVIP 509
            +   +F  GLK+L I C  S GG YCW DMS L+ SYSEKGELEL+EKL+ + K+N  P
Sbjct: 421 RDKHIRFVEGLKQLGIPCAESGGGLYCWVDMSSLLTSYSEKGELELFEKLLTVAKINATP 480

Query: 510 GSSCNCIEPGWFGFCFTTLIDKEIPIVMDRIRRISEGCKS 548
           G++C CIEPGWF  CFT L D++IP++M+RIR+++E  +S
Sbjct: 481 GTACYCIEPGWFRCCFTALADEDIPVIMERIRQLAESFRS 495

BLAST of Bhi12G000329 vs. ExPASy Swiss-Prot
Match: P37821 (1-aminocyclopropane-1-carboxylate synthase OS=Malus domestica OX=3750 GN=ACS-1 PE=1 SV=2)

HSP 1 Score: 386.7 bits (992), Expect = 4.3e-106
Identity = 182/425 (42.82%), Postives = 277/425 (65.18%), Query Frame = 0

Query: 120 VSGRANSISRVGDSTIFVGMRRFLEDPFDELDNPTGIIQLSVAENKLSSDLVQNWLTENG 179
           +S  A   S   DS+ F+G + + ++P+ E+ N  GIIQ+ +AEN+L  DL+++WL +N 
Sbjct: 4   LSRNATFNSHGQDSSYFLGWQEYEKNPYHEVHNTNGIIQMGLAENQLCFDLLESWLAKNP 63

Query: 180 RDAILGGVNHNELSVAGTVSYRPSDGLTDLKLAVASFMSQVVGNSVSFIPSHLVFTTGVT 239
             A      + E   A    ++   GL   K A+  FM+++ GN V+F P+HLV T G T
Sbjct: 64  EAAAF--KKNGESIFAELALFQDYHGLPAFKKAMVDFMAEIRGNKVTFDPNHLVLTAGAT 123

Query: 240 SAIETLCFCIADPGNAFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSITSLDRA 299
           SA ET  FC+ADPG A L+PTPYYPG D D+KWRTGVEIVP+ C S++ F ++ T+L+ A
Sbjct: 124 SANETFIFCLADPGEAVLIPTPYYPGFDRDLKWRTGVEIVPIHCTSSNGFQITETALEEA 183

Query: 300 FHQARNRGLKVRGIIISNPSIPVGNQLNRETLYDLLDFAREKNIHIISNEIFVGSTYGSE 359
           + +A  R L+V+G++++NPS P+G  + R  LY LL F  +K IH+IS+EI+ G+ + S 
Sbjct: 184 YQEAEKRNLRVKGVLVTNPSNPLGTTMTRNELYLLLSFVEDKGIHLISDEIYSGTAFSSP 243

Query: 360 EFVSMAEIIDAEDSDRD-----RVHITYALSEDMSLPGFGVAAIYSLNENILAAARKLAR 419
            F+S+ E++   + D +     RVH+ Y+LS+D+ LPGF V AIYS ++ ++AAA K++ 
Sbjct: 244 SFISVMEVLKDRNCDENSEVWQRVHVVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 303

Query: 420 FSSVSAITQNLLSSMLSDTQFIQRFIRTNRERLGEMDGKFTAGLKELNIKCMRSNGGFYC 479
           F  VS+ TQ+LLS+MLSD +  + +I  N +RL +   K  +GL++  I C+  N G +C
Sbjct: 304 FGLVSSQTQHLLSAMLSDKKLTKNYIAENHKRLKQRQKKLVSGLQKSGISCLNGNAGLFC 363

Query: 480 WADMSKLIRSYSEKGELELWEKLVDIGKVNVIPGSSCNCIEPGWFGFCFTTLIDKEIPIV 539
           W DM  L+RS + + E+ELW+K+V    +N+ PGSSC+C EPGWF  CF  L ++ + + 
Sbjct: 364 WVDMRHLLRSNTFEAEMELWKKIVYEVHLNISPGSSCHCTEPGWFRVCFANLPERTLDLA 423

BLAST of Bhi12G000329 vs. ExPASy Swiss-Prot
Match: Q9T065 (1-aminocyclopropane-1-carboxylate synthase 8 OS=Arabidopsis thaliana OX=3702 GN=ACS8 PE=1 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 9.6e-106
Identity = 190/432 (43.98%), Postives = 286/432 (66.20%), Query Frame = 0

Query: 120 VSGRANSISRVGDSTIFVGMRRFLEDPFDELDNPTGIIQLSVAENKLSSDLVQNWLTENG 179
           +S +A+  +   DS+ F G   + ++P+DE+ NP GIIQ+ +AEN+LS DL+++WL +N 
Sbjct: 4   LSKKASCNTHGQDSSYFWGWEEYEKNPYDEIKNPDGIIQMGLAENQLSFDLIESWLAKNP 63

Query: 180 RDAIL---GGVNHNELSVAGTVSYRPSDGLTDLKLAVASFMSQVVGNSVSFIPSHLVFTT 239
             A     G     EL++     ++   GL   K A+A FMS+  GN VSF P+ LV T 
Sbjct: 64  DAANFQREGQSIFRELAL-----FQDYHGLPSFKNAMADFMSENRGNRVSFNPNKLVLTA 123

Query: 240 GVTSAIETLCFCIADPGNAFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSITSL 299
           G T A ETL FC+ADPG+AFL+PTPYYPG D D+KWRTG EIVP+ C+SA+ F ++  +L
Sbjct: 124 GATPANETLMFCLADPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIQCKSANGFRITKVAL 183

Query: 300 DRAFHQARNRGLKVRGIIISNPSIPVGNQLNRETLYDLLDFAREKNIHIISNEIFVGSTY 359
           + A+ QA+   LKV+G++I+NPS P+G    R  L  LLDF   K IH+IS+EI+ G+ +
Sbjct: 184 EEAYEQAQKLNLKVKGVLITNPSNPLGTTTTRTELNHLLDFISRKKIHLISDEIYSGTVF 243

Query: 360 GSEEFVSMAEII---DAEDSD-RDRVHITYALSEDMSLPGFGVAAIYSLNENILAAARKL 419
            +  F+S+ E++     E++D  DRVHI Y+LS+D+ LPGF V  IYS ++ +++AA K+
Sbjct: 244 TNPGFISVMEVLKDRKLENTDVFDRVHIVYSLSKDLGLPGFRVGVIYSNDDFVVSAATKM 303

Query: 420 ARFSSVSAITQNLLSSMLSDTQFIQRFIRTNRERLGEMDGKFTAGLKELNIKCMRSNGGF 479
           + F  +S+ TQ LLS++LSD  F + ++  N+ RL     K  +GL+   I+C++SN G 
Sbjct: 304 SSFGLISSQTQYLLSALLSDKTFTKNYLEENQIRLKNRHKKLVSGLEAAGIECLKSNAGL 363

Query: 480 YCWADMSKLIRSYSEKGELELWEKLVDIGKVNVIPGSSCNCIEPGWFGFCFTTLIDKEIP 539
           +CW DM  L++S + + E+ELW+K+V   K+N+ PGSSC+C EPGWF  CF  L ++ + 
Sbjct: 364 FCWVDMRHLLKSNTFEAEIELWKKIVYEVKLNISPGSSCHCNEPGWFRVCFANLSEETLK 423

Query: 540 IVMDRIRRISEG 545
           + +DR++R  +G
Sbjct: 424 VALDRLKRFVDG 430

BLAST of Bhi12G000329 vs. ExPASy Swiss-Prot
Match: Q9M2Y8 (1-aminocyclopropane-1-carboxylate synthase 9 OS=Arabidopsis thaliana OX=3702 GN=ACS9 PE=1 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 2.1e-105
Identity = 187/433 (43.19%), Postives = 288/433 (66.51%), Query Frame = 0

Query: 120 VSGRANSISRVGDSTIFVGMRRFLEDPFDELDNPTGIIQLSVAENKLSSDLVQNWLTENG 179
           +S +  S +   DS+ F+G   + ++P+DE+ NP GIIQ+ +AEN+L  DL++ WL +N 
Sbjct: 4   LSRKVTSNAHGQDSSYFLGWEEYEKNPYDEIKNPNGIIQMGLAENQLCFDLIETWLAKNP 63

Query: 180 RDAILGGVNHNELSVAGTVS-YRPSDGLTDLKLAVASFMSQVVGNSVSFIPSHLVFTTGV 239
             A   G+  +  S+   ++ ++   GL + K A+A FM ++ GN V+F PS +V   G 
Sbjct: 64  DAA---GLKKDGQSIFKELALFQDYHGLPEFKKALAEFMEEIRGNRVTFDPSKIVLAAGS 123

Query: 240 TSAIETLCFCIADPGNAFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSITSLDR 299
           TSA ETL FC+A+PG+AFL+PTPYYPG D D+KWRTG EIVP+ C S++ F ++ ++L +
Sbjct: 124 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFQITESALQQ 183

Query: 300 AFHQARNRGLKVRGIIISNPSIPVGNQLNRETLYDLLDFAREKNIHIISNEIFVGSTYGS 359
           A+ QA+   LKV+G++++NPS P+G  L R  L  L+DF   KNIH+IS+EI+ G+ +G 
Sbjct: 184 AYQQAQKLDLKVKGVLVTNPSNPLGTMLTRRELNLLVDFITSKNIHLISDEIYSGTVFGF 243

Query: 360 EEFVSMAEII---DAEDSD-RDRVHITYALSEDMSLPGFGVAAIYSLNENILAAARKLAR 419
           E+FVS+ +++   + E+S+   RVHI Y+LS+D+ LPGF V AIYS +E +++AA K++ 
Sbjct: 244 EQFVSVMDVLKDKNLENSEVSKRVHIVYSLSKDLGLPGFRVGAIYSNDEMVVSAATKMSS 303

Query: 420 FSSVSAITQNLLSSMLSDTQFIQRFIRTNRERLGEMDGKFTAGLKELNIKCMRSNGGFYC 479
           F  VS+ TQ LLS++LSD +F   ++  N++RL     K  +GL+   I C++SN G +C
Sbjct: 304 FGLVSSQTQYLLSALLSDKKFTSTYLDENQKRLKIRQKKLVSGLEAAGITCLKSNAGLFC 363

Query: 480 WADMSKLIRSYSEKGELELWEKLVDIGKVNVIPGSSCNCIEPGWFGFCFTTLIDKEIPIV 539
           W DM  L+ + + + ELELW+K+V   K+N+ PGSSC+C EPGWF  CF  + +  + + 
Sbjct: 364 WVDMRHLLDTNTFEAELELWKKIVYDVKLNISPGSSCHCTEPGWFRVCFANMSEDTLDLA 423

Query: 540 MDRIRRISEGCKS 548
           M R++   E   S
Sbjct: 424 MKRLKEYVESTDS 433

BLAST of Bhi12G000329 vs. NCBI nr
Match: XP_038874650.1 (probable aminotransferase ACS10 [Benincasa hispida])

HSP 1 Score: 1087.4 bits (2811), Expect = 0.0e+00
Identity = 549/549 (100.00%), Postives = 549/549 (100.00%), Query Frame = 0

Query: 1   MTQTRFFRTRNSKSDKEAEPTTSGGGGAAMRVIVPLQGVVQGRGGVVLGSVIPCVLFYFL 60
           MTQTRFFRTRNSKSDKEAEPTTSGGGGAAMRVIVPLQGVVQGRGGVVLGSVIPCVLFYFL
Sbjct: 1   MTQTRFFRTRNSKSDKEAEPTTSGGGGAAMRVIVPLQGVVQGRGGVVLGSVIPCVLFYFL 60

Query: 61  QLYLKRNRSEPSPDPPSSQEVDSSGNSSPSSSNQLVELSVLPRTLSRTFLSPRSSGPLCV 120
           QLYLKRNRSEPSPDPPSSQEVDSSGNSSPSSSNQLVELSVLPRTLSRTFLSPRSSGPLCV
Sbjct: 61  QLYLKRNRSEPSPDPPSSQEVDSSGNSSPSSSNQLVELSVLPRTLSRTFLSPRSSGPLCV 120

Query: 121 SGRANSISRVGDSTIFVGMRRFLEDPFDELDNPTGIIQLSVAENKLSSDLVQNWLTENGR 180
           SGRANSISRVGDSTIFVGMRRFLEDPFDELDNPTGIIQLSVAENKLSSDLVQNWLTENGR
Sbjct: 121 SGRANSISRVGDSTIFVGMRRFLEDPFDELDNPTGIIQLSVAENKLSSDLVQNWLTENGR 180

Query: 181 DAILGGVNHNELSVAGTVSYRPSDGLTDLKLAVASFMSQVVGNSVSFIPSHLVFTTGVTS 240
           DAILGGVNHNELSVAGTVSYRPSDGLTDLKLAVASFMSQVVGNSVSFIPSHLVFTTGVTS
Sbjct: 181 DAILGGVNHNELSVAGTVSYRPSDGLTDLKLAVASFMSQVVGNSVSFIPSHLVFTTGVTS 240

Query: 241 AIETLCFCIADPGNAFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSITSLDRAF 300
           AIETLCFCIADPGNAFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSITSLDRAF
Sbjct: 241 AIETLCFCIADPGNAFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSITSLDRAF 300

Query: 301 HQARNRGLKVRGIIISNPSIPVGNQLNRETLYDLLDFAREKNIHIISNEIFVGSTYGSEE 360
           HQARNRGLKVRGIIISNPSIPVGNQLNRETLYDLLDFAREKNIHIISNEIFVGSTYGSEE
Sbjct: 301 HQARNRGLKVRGIIISNPSIPVGNQLNRETLYDLLDFAREKNIHIISNEIFVGSTYGSEE 360

Query: 361 FVSMAEIIDAEDSDRDRVHITYALSEDMSLPGFGVAAIYSLNENILAAARKLARFSSVSA 420
           FVSMAEIIDAEDSDRDRVHITYALSEDMSLPGFGVAAIYSLNENILAAARKLARFSSVSA
Sbjct: 361 FVSMAEIIDAEDSDRDRVHITYALSEDMSLPGFGVAAIYSLNENILAAARKLARFSSVSA 420

Query: 421 ITQNLLSSMLSDTQFIQRFIRTNRERLGEMDGKFTAGLKELNIKCMRSNGGFYCWADMSK 480
           ITQNLLSSMLSDTQFIQRFIRTNRERLGEMDGKFTAGLKELNIKCMRSNGGFYCWADMSK
Sbjct: 421 ITQNLLSSMLSDTQFIQRFIRTNRERLGEMDGKFTAGLKELNIKCMRSNGGFYCWADMSK 480

Query: 481 LIRSYSEKGELELWEKLVDIGKVNVIPGSSCNCIEPGWFGFCFTTLIDKEIPIVMDRIRR 540
           LIRSYSEKGELELWEKLVDIGKVNVIPGSSCNCIEPGWFGFCFTTLIDKEIPIVMDRIRR
Sbjct: 481 LIRSYSEKGELELWEKLVDIGKVNVIPGSSCNCIEPGWFGFCFTTLIDKEIPIVMDRIRR 540

Query: 541 ISEGCKSYT 550
           ISEGCKSYT
Sbjct: 541 ISEGCKSYT 549

BLAST of Bhi12G000329 vs. NCBI nr
Match: XP_004147561.1 (probable aminotransferase ACS10 [Cucumis sativus] >KGN50179.1 hypothetical protein Csa_000690 [Cucumis sativus])

HSP 1 Score: 1016.1 bits (2626), Expect = 1.1e-292
Identity = 515/549 (93.81%), Postives = 529/549 (96.36%), Query Frame = 0

Query: 1   MTQTRFFRTRNSKSDKEAEPTTSGGGGAAMRVIVPLQGVVQGRGGVVLGSVIPCVLFYFL 60
           MTQTRFFRTRNSKSDKEAEPTTS GGGAAMRVIVPLQGVVQGRGGVVLGSVIPCVLFYFL
Sbjct: 1   MTQTRFFRTRNSKSDKEAEPTTSSGGGAAMRVIVPLQGVVQGRGGVVLGSVIPCVLFYFL 60

Query: 61  QLYLKRNRSEPSPDPPSSQEVDSSGNSSPSSSNQLVELSVLPRTLSRTFLSPRSSGPLCV 120
           QLYLKRNRS+P+ DPPSS EVDSSG+SSPSSSNQ VELSVLPRTLSR FLSPRSSGP+CV
Sbjct: 61  QLYLKRNRSDPTRDPPSSPEVDSSGDSSPSSSNQFVELSVLPRTLSRNFLSPRSSGPVCV 120

Query: 121 SGRANSISRVGDSTIFVGMRRFLEDPFDELDNPTGIIQLSVAENKLSSDLVQNWLTENGR 180
           SGRANSISRVGDS+IFVGMRRFLED FDELDNPTGIIQLSVAENKLSSDLVQNWLTENGR
Sbjct: 121 SGRANSISRVGDSSIFVGMRRFLEDSFDELDNPTGIIQLSVAENKLSSDLVQNWLTENGR 180

Query: 181 DAILGGVNHNELSVAGTVSYRPSDGLTDLKLAVASFMSQVVGNSVSFIPSHLVFTTGVTS 240
           DAIL GVNHNELSVAGTV Y+PSDGL DLKLAVASFMSQVVGNSVSF PS LVFTTGVTS
Sbjct: 181 DAIL-GVNHNELSVAGTVCYQPSDGLKDLKLAVASFMSQVVGNSVSFNPSQLVFTTGVTS 240

Query: 241 AIETLCFCIADPGNAFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSITSLDRAF 300
           AIETLCFCIADPGNAFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSITSLDRAF
Sbjct: 241 AIETLCFCIADPGNAFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSITSLDRAF 300

Query: 301 HQARNRGLKVRGIIISNPSIPVGNQLNRETLYDLLDFAREKNIHIISNEIFVGSTYGSEE 360
           HQARNRGLKVRGIIISNPSIPVGNQLNRETLYDLLDFAREKNIHIISNEIFVGSTYGSEE
Sbjct: 301 HQARNRGLKVRGIIISNPSIPVGNQLNRETLYDLLDFAREKNIHIISNEIFVGSTYGSEE 360

Query: 361 FVSMAEIIDAEDSDRDRVHITYALSEDMSLPGFGVAAIYSLNENILAAARKLARFSSVSA 420
           FVSMAE+I+ ED+DRDRVHITY LSEDMSLPGF VAAIYSLNENILAAARKLARFSSVSA
Sbjct: 361 FVSMAEMINLEDNDRDRVHITYGLSEDMSLPGFRVAAIYSLNENILAAARKLARFSSVSA 420

Query: 421 ITQNLLSSMLSDTQFIQRFIRTNRERLGEMDGKFTAGLKELNIKCMRSNGGFYCWADMSK 480
           ITQNLLSSMLSDTQFIQRFIRTNRERLGEM+ KFTAGLKEL+IKC +SNGGFYCWADMSK
Sbjct: 421 ITQNLLSSMLSDTQFIQRFIRTNRERLGEMNSKFTAGLKELDIKCAKSNGGFYCWADMSK 480

Query: 481 LIRSYSEKGELELWEKLVDIGKVNVIPGSSCNCIEPGWFGFCFTTLIDKEIPIVMDRIRR 540
           LIRSYSEKGELELWEKL+DIGKV+V PGS CNCIEPGWFGFCFT LIDKEIPIVMDRIRR
Sbjct: 481 LIRSYSEKGELELWEKLLDIGKVSVTPGSCCNCIEPGWFGFCFTALIDKEIPIVMDRIRR 540

Query: 541 ISEGCKSYT 550
           ISE CKSYT
Sbjct: 541 ISEACKSYT 548

BLAST of Bhi12G000329 vs. NCBI nr
Match: XP_008437168.1 (PREDICTED: probable aminotransferase ACS10 [Cucumis melo])

HSP 1 Score: 1013.4 bits (2619), Expect = 7.1e-292
Identity = 511/549 (93.08%), Postives = 529/549 (96.36%), Query Frame = 0

Query: 1   MTQTRFFRTRNSKSDKEAEPTTSGGGGAAMRVIVPLQGVVQGRGGVVLGSVIPCVLFYFL 60
           MTQTRFFRTRNSKSDKEAEPTTS GGGAAMRVIVPLQGVVQGRGGVVLGSVIPCVLFYFL
Sbjct: 1   MTQTRFFRTRNSKSDKEAEPTTSSGGGAAMRVIVPLQGVVQGRGGVVLGSVIPCVLFYFL 60

Query: 61  QLYLKRNRSEPSPDPPSSQEVDSSGNSSPSSSNQLVELSVLPRTLSRTFLSPRSSGPLCV 120
           QLYLKRNRS+P+ DPPSS EVDSSG+SSPSSSNQLVELSVLPRTLSR FLSPRS GP+CV
Sbjct: 61  QLYLKRNRSDPTRDPPSSPEVDSSGDSSPSSSNQLVELSVLPRTLSRNFLSPRSCGPVCV 120

Query: 121 SGRANSISRVGDSTIFVGMRRFLEDPFDELDNPTGIIQLSVAENKLSSDLVQNWLTENGR 180
           SGRANSISR+G+S+IFVGMRRFLEDPFDELDNPTGIIQLSVAENKLSSDLVQNWLTENGR
Sbjct: 121 SGRANSISRLGESSIFVGMRRFLEDPFDELDNPTGIIQLSVAENKLSSDLVQNWLTENGR 180

Query: 181 DAILGGVNHNELSVAGTVSYRPSDGLTDLKLAVASFMSQVVGNSVSFIPSHLVFTTGVTS 240
           DAIL G+NHNELSVAGTV Y+PSDGL DLKLAVASFMSQVVG+SVSF PS LVFTTGVTS
Sbjct: 181 DAIL-GMNHNELSVAGTVCYQPSDGLKDLKLAVASFMSQVVGSSVSFNPSQLVFTTGVTS 240

Query: 241 AIETLCFCIADPGNAFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSITSLDRAF 300
           AIETLCFCIADPGNAFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSITSLDRAF
Sbjct: 241 AIETLCFCIADPGNAFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSITSLDRAF 300

Query: 301 HQARNRGLKVRGIIISNPSIPVGNQLNRETLYDLLDFAREKNIHIISNEIFVGSTYGSEE 360
           HQARNRGLKVRGIIISNPSIPVGNQLNRETLYDLLDFAREKNIHIISNEIFVGSTYGSEE
Sbjct: 301 HQARNRGLKVRGIIISNPSIPVGNQLNRETLYDLLDFAREKNIHIISNEIFVGSTYGSEE 360

Query: 361 FVSMAEIIDAEDSDRDRVHITYALSEDMSLPGFGVAAIYSLNENILAAARKLARFSSVSA 420
           FVSMAE+ID ED+DRDRVHITY LSEDMSLPGF VAAIYSLNENILAAARKLARFSSVSA
Sbjct: 361 FVSMAEMIDLEDNDRDRVHITYGLSEDMSLPGFRVAAIYSLNENILAAARKLARFSSVSA 420

Query: 421 ITQNLLSSMLSDTQFIQRFIRTNRERLGEMDGKFTAGLKELNIKCMRSNGGFYCWADMSK 480
           ITQNLLSSMLSDTQFIQ FIRTNRERLGEM+ KFTAGLKEL+IKC++SNGGFYCWADMSK
Sbjct: 421 ITQNLLSSMLSDTQFIQSFIRTNRERLGEMNSKFTAGLKELDIKCVKSNGGFYCWADMSK 480

Query: 481 LIRSYSEKGELELWEKLVDIGKVNVIPGSSCNCIEPGWFGFCFTTLIDKEIPIVMDRIRR 540
           LIRSYSEKGELELWEKL D+GKVNV PGS CNCIEPGWFGFCFTT +DKEIPIVMDRIRR
Sbjct: 481 LIRSYSEKGELELWEKLFDVGKVNVTPGSCCNCIEPGWFGFCFTTSVDKEIPIVMDRIRR 540

Query: 541 ISEGCKSYT 550
           ISE CKSYT
Sbjct: 541 ISEACKSYT 548

BLAST of Bhi12G000329 vs. NCBI nr
Match: ASV63085.1 (1-aminocyclopropane-1-carboxylic acid synthase [Siraitia grosvenorii])

HSP 1 Score: 974.2 bits (2517), Expect = 4.8e-280
Identity = 489/549 (89.07%), Postives = 516/549 (93.99%), Query Frame = 0

Query: 1   MTQTRFFRTRNSKSDKEAEPTTSGGGGAAMRVIVPLQGVVQGRGGVVLGSVIPCVLFYFL 60
           MTQTRFFRTRN KSD+EAEPTTSGGGGAAMRVIVPLQGVVQGRGGVVLGSVIPCVLFYFL
Sbjct: 1   MTQTRFFRTRNPKSDEEAEPTTSGGGGAAMRVIVPLQGVVQGRGGVVLGSVIPCVLFYFL 60

Query: 61  QLYLKRNRSEPSPDPPSSQEVDSSGNSSPSSSNQLVELSVLPRTLSRTFLSPRSSGPLCV 120
           QLYLKRNRS+P+ DPP S EV S GNSSP+S N LVELSVLPRTLSRTFLSPRSSGP+CV
Sbjct: 61  QLYLKRNRSDPNRDPPPSPEVASPGNSSPTSGNPLVELSVLPRTLSRTFLSPRSSGPVCV 120

Query: 121 SGRANSISRVGDSTIFVGMRRFLEDPFDELDNPTGIIQLSVAENKLSSDLVQNWLTENGR 180
           SGRANSI+R GDS+ F+GMR+FLEDP+DELDNPTGIIQL VAENKLSSDLVQNWLTENGR
Sbjct: 121 SGRANSIARGGDSSFFLGMRKFLEDPYDELDNPTGIIQLCVAENKLSSDLVQNWLTENGR 180

Query: 181 DAILGGVNHNELSVAGTVSYRPSDGLTDLKLAVASFMSQVVGNSVSFIPSHLVFTTGVTS 240
           DAILGG NHN LSVAGT SY+P DGLTDLKLAVA FMSQ VGNSVSF PS LVFTTGVTS
Sbjct: 181 DAILGG-NHNGLSVAGTASYQPMDGLTDLKLAVAGFMSQAVGNSVSFSPSQLVFTTGVTS 240

Query: 241 AIETLCFCIADPGNAFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSITSLDRAF 300
           AIETLCFCIADPG+AFLVP+PYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSI SLDRAF
Sbjct: 241 AIETLCFCIADPGHAFLVPSPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSINSLDRAF 300

Query: 301 HQARNRGLKVRGIIISNPSIPVGNQLNRETLYDLLDFAREKNIHIISNEIFVGSTYGSEE 360
           HQA+ RGLKVRGIIISNPSIPVGNQL+RETLY++LDFAREKNIHIISNEIFVGSTYGSEE
Sbjct: 301 HQAKKRGLKVRGIIISNPSIPVGNQLHRETLYNILDFAREKNIHIISNEIFVGSTYGSEE 360

Query: 361 FVSMAEIIDAEDSDRDRVHITYALSEDMSLPGFGVAAIYSLNENILAAARKLARFSSVSA 420
           FVSMAE+ID EDSDRDRVHI Y LSED+SLPGF VAAIYSLNENILAAA+KLARFSSVSA
Sbjct: 361 FVSMAEMIDLEDSDRDRVHIAYGLSEDLSLPGFRVAAIYSLNENILAAAKKLARFSSVSA 420

Query: 421 ITQNLLSSMLSDTQFIQRFIRTNRERLGEMDGKFTAGLKELNIKCMRSNGGFYCWADMSK 480
           ITQNLLSSML DT F+QRFIR NRERLGEM GKF AGLKEL+IKCM+SNGGFYCWADMSK
Sbjct: 421 ITQNLLSSMLLDTVFVQRFIRINRERLGEMHGKFVAGLKELDIKCMKSNGGFYCWADMSK 480

Query: 481 LIRSYSEKGELELWEKLVDIGKVNVIPGSSCNCIEPGWFGFCFTTLIDKEIPIVMDRIRR 540
           LIRSYSEKGELELW+KL+DIGKVNV PGSSCNCIE GWFGFCFTTLID+EIPIVMDRIRR
Sbjct: 481 LIRSYSEKGELELWDKLLDIGKVNVAPGSSCNCIELGWFGFCFTTLIDREIPIVMDRIRR 540

Query: 541 ISEGCKSYT 550
           ISE CKSY+
Sbjct: 541 ISEACKSYS 548

BLAST of Bhi12G000329 vs. NCBI nr
Match: XP_023532648.1 (probable aminotransferase ACS10 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 956.4 bits (2471), Expect = 1.0e-274
Identity = 480/549 (87.43%), Postives = 510/549 (92.90%), Query Frame = 0

Query: 1   MTQTRFFRTRNSKSDKEAEPTTSGGGGAAMRVIVPLQGVVQGRGGVVLGSVIPCVLFYFL 60
           MT TRFFR RNSKS+KE EPTTS GGG AMRVIVPLQG+VQG GGVVLGS+IPCVLFYFL
Sbjct: 1   MTHTRFFRNRNSKSNKEGEPTTSDGGGTAMRVIVPLQGIVQGSGGVVLGSLIPCVLFYFL 60

Query: 61  QLYLKRNRSEPSPDPPSSQEVDSSGNSSPSSSNQLVELSVLPRTLSRTFLSPRSSGPLCV 120
           QLYLKRNRSE + DPPSS EVDS GNSS  SSNQ VELS LPRTLSRTFLS RS+GP+CV
Sbjct: 61  QLYLKRNRSEATEDPPSSPEVDSPGNSSSPSSNQSVELSTLPRTLSRTFLSSRSNGPVCV 120

Query: 121 SGRANSISRVGDSTIFVGMRRFLEDPFDELDNPTGIIQLSVAENKLSSDLVQNWLTENGR 180
           SGRANSI++VG+S+IFVGM++F EDPFDELDNPTGIIQLS+AENKLSSDLVQNWL ENGR
Sbjct: 121 SGRANSIAKVGESSIFVGMKKFFEDPFDELDNPTGIIQLSIAENKLSSDLVQNWLVENGR 180

Query: 181 DAILGGVNHNELSVAGTVSYRPSDGLTDLKLAVASFMSQVVGNSVSFIPSHLVFTTGVTS 240
           DAILG  NHNELSVAGTVSY+PSDGL DLKLAVASFMSQ VGNSVSFIPS +VFT GV+S
Sbjct: 181 DAILGVGNHNELSVAGTVSYQPSDGLMDLKLAVASFMSQAVGNSVSFIPSQIVFTNGVSS 240

Query: 241 AIETLCFCIADPGNAFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSITSLDRAF 300
           AIETLCFCIAD G+AFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSITSLDRAF
Sbjct: 241 AIETLCFCIADAGHAFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSITSLDRAF 300

Query: 301 HQARNRGLKVRGIIISNPSIPVGNQLNRETLYDLLDFAREKNIHIISNEIFVGSTYGSEE 360
           HQAR+RGLKVRGIIISNPSIPVGNQL++ETLY LLDFAR+KNIHIISNEIFVGSTYGSEE
Sbjct: 301 HQARSRGLKVRGIIISNPSIPVGNQLHKETLYKLLDFARDKNIHIISNEIFVGSTYGSEE 360

Query: 361 FVSMAEIIDAEDSDRDRVHITYALSEDMSLPGFGVAAIYSLNENILAAARKLARFSSVSA 420
           FVSMAE+ID EDSDRDRVHITY LSEDMSLPGF VAAIYSLNENILAAA KLARFSSVSA
Sbjct: 361 FVSMAEMIDLEDSDRDRVHITYGLSEDMSLPGFRVAAIYSLNENILAAAGKLARFSSVSA 420

Query: 421 ITQNLLSSMLSDTQFIQRFIRTNRERLGEMDGKFTAGLKELNIKCMRSNGGFYCWADMSK 480
           ITQNLLSSMLSD QFIQ+FI  NR+RLGEM+ KF AGLKEL+IK MRSNGGFYCWADM  
Sbjct: 421 ITQNLLSSMLSDAQFIQKFIGINRKRLGEMNSKFVAGLKELDIKYMRSNGGFYCWADMGN 480

Query: 481 LIRSYSEKGELELWEKLVDIGKVNVIPGSSCNCIEPGWFGFCFTTLIDKEIPIVMDRIRR 540
           LIRSYSEKGE+ELW+KL+DIGKV+VIPGS CNCIEPGWFGFCFTTLIDKEIPIVMDRIRR
Sbjct: 481 LIRSYSEKGEVELWDKLLDIGKVHVIPGSCCNCIEPGWFGFCFTTLIDKEIPIVMDRIRR 540

Query: 541 ISEGCKSYT 550
           ISE CKSYT
Sbjct: 541 ISEACKSYT 549

BLAST of Bhi12G000329 vs. ExPASy TrEMBL
Match: A0A0A0KMR4 (Aminotran_1_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G157380 PE=4 SV=1)

HSP 1 Score: 1016.1 bits (2626), Expect = 5.3e-293
Identity = 515/549 (93.81%), Postives = 529/549 (96.36%), Query Frame = 0

Query: 1   MTQTRFFRTRNSKSDKEAEPTTSGGGGAAMRVIVPLQGVVQGRGGVVLGSVIPCVLFYFL 60
           MTQTRFFRTRNSKSDKEAEPTTS GGGAAMRVIVPLQGVVQGRGGVVLGSVIPCVLFYFL
Sbjct: 1   MTQTRFFRTRNSKSDKEAEPTTSSGGGAAMRVIVPLQGVVQGRGGVVLGSVIPCVLFYFL 60

Query: 61  QLYLKRNRSEPSPDPPSSQEVDSSGNSSPSSSNQLVELSVLPRTLSRTFLSPRSSGPLCV 120
           QLYLKRNRS+P+ DPPSS EVDSSG+SSPSSSNQ VELSVLPRTLSR FLSPRSSGP+CV
Sbjct: 61  QLYLKRNRSDPTRDPPSSPEVDSSGDSSPSSSNQFVELSVLPRTLSRNFLSPRSSGPVCV 120

Query: 121 SGRANSISRVGDSTIFVGMRRFLEDPFDELDNPTGIIQLSVAENKLSSDLVQNWLTENGR 180
           SGRANSISRVGDS+IFVGMRRFLED FDELDNPTGIIQLSVAENKLSSDLVQNWLTENGR
Sbjct: 121 SGRANSISRVGDSSIFVGMRRFLEDSFDELDNPTGIIQLSVAENKLSSDLVQNWLTENGR 180

Query: 181 DAILGGVNHNELSVAGTVSYRPSDGLTDLKLAVASFMSQVVGNSVSFIPSHLVFTTGVTS 240
           DAIL GVNHNELSVAGTV Y+PSDGL DLKLAVASFMSQVVGNSVSF PS LVFTTGVTS
Sbjct: 181 DAIL-GVNHNELSVAGTVCYQPSDGLKDLKLAVASFMSQVVGNSVSFNPSQLVFTTGVTS 240

Query: 241 AIETLCFCIADPGNAFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSITSLDRAF 300
           AIETLCFCIADPGNAFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSITSLDRAF
Sbjct: 241 AIETLCFCIADPGNAFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSITSLDRAF 300

Query: 301 HQARNRGLKVRGIIISNPSIPVGNQLNRETLYDLLDFAREKNIHIISNEIFVGSTYGSEE 360
           HQARNRGLKVRGIIISNPSIPVGNQLNRETLYDLLDFAREKNIHIISNEIFVGSTYGSEE
Sbjct: 301 HQARNRGLKVRGIIISNPSIPVGNQLNRETLYDLLDFAREKNIHIISNEIFVGSTYGSEE 360

Query: 361 FVSMAEIIDAEDSDRDRVHITYALSEDMSLPGFGVAAIYSLNENILAAARKLARFSSVSA 420
           FVSMAE+I+ ED+DRDRVHITY LSEDMSLPGF VAAIYSLNENILAAARKLARFSSVSA
Sbjct: 361 FVSMAEMINLEDNDRDRVHITYGLSEDMSLPGFRVAAIYSLNENILAAARKLARFSSVSA 420

Query: 421 ITQNLLSSMLSDTQFIQRFIRTNRERLGEMDGKFTAGLKELNIKCMRSNGGFYCWADMSK 480
           ITQNLLSSMLSDTQFIQRFIRTNRERLGEM+ KFTAGLKEL+IKC +SNGGFYCWADMSK
Sbjct: 421 ITQNLLSSMLSDTQFIQRFIRTNRERLGEMNSKFTAGLKELDIKCAKSNGGFYCWADMSK 480

Query: 481 LIRSYSEKGELELWEKLVDIGKVNVIPGSSCNCIEPGWFGFCFTTLIDKEIPIVMDRIRR 540
           LIRSYSEKGELELWEKL+DIGKV+V PGS CNCIEPGWFGFCFT LIDKEIPIVMDRIRR
Sbjct: 481 LIRSYSEKGELELWEKLLDIGKVSVTPGSCCNCIEPGWFGFCFTALIDKEIPIVMDRIRR 540

Query: 541 ISEGCKSYT 550
           ISE CKSYT
Sbjct: 541 ISEACKSYT 548

BLAST of Bhi12G000329 vs. ExPASy TrEMBL
Match: A0A1S3ATY5 (probable aminotransferase ACS10 OS=Cucumis melo OX=3656 GN=LOC103482676 PE=4 SV=1)

HSP 1 Score: 1013.4 bits (2619), Expect = 3.5e-292
Identity = 511/549 (93.08%), Postives = 529/549 (96.36%), Query Frame = 0

Query: 1   MTQTRFFRTRNSKSDKEAEPTTSGGGGAAMRVIVPLQGVVQGRGGVVLGSVIPCVLFYFL 60
           MTQTRFFRTRNSKSDKEAEPTTS GGGAAMRVIVPLQGVVQGRGGVVLGSVIPCVLFYFL
Sbjct: 1   MTQTRFFRTRNSKSDKEAEPTTSSGGGAAMRVIVPLQGVVQGRGGVVLGSVIPCVLFYFL 60

Query: 61  QLYLKRNRSEPSPDPPSSQEVDSSGNSSPSSSNQLVELSVLPRTLSRTFLSPRSSGPLCV 120
           QLYLKRNRS+P+ DPPSS EVDSSG+SSPSSSNQLVELSVLPRTLSR FLSPRS GP+CV
Sbjct: 61  QLYLKRNRSDPTRDPPSSPEVDSSGDSSPSSSNQLVELSVLPRTLSRNFLSPRSCGPVCV 120

Query: 121 SGRANSISRVGDSTIFVGMRRFLEDPFDELDNPTGIIQLSVAENKLSSDLVQNWLTENGR 180
           SGRANSISR+G+S+IFVGMRRFLEDPFDELDNPTGIIQLSVAENKLSSDLVQNWLTENGR
Sbjct: 121 SGRANSISRLGESSIFVGMRRFLEDPFDELDNPTGIIQLSVAENKLSSDLVQNWLTENGR 180

Query: 181 DAILGGVNHNELSVAGTVSYRPSDGLTDLKLAVASFMSQVVGNSVSFIPSHLVFTTGVTS 240
           DAIL G+NHNELSVAGTV Y+PSDGL DLKLAVASFMSQVVG+SVSF PS LVFTTGVTS
Sbjct: 181 DAIL-GMNHNELSVAGTVCYQPSDGLKDLKLAVASFMSQVVGSSVSFNPSQLVFTTGVTS 240

Query: 241 AIETLCFCIADPGNAFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSITSLDRAF 300
           AIETLCFCIADPGNAFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSITSLDRAF
Sbjct: 241 AIETLCFCIADPGNAFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSITSLDRAF 300

Query: 301 HQARNRGLKVRGIIISNPSIPVGNQLNRETLYDLLDFAREKNIHIISNEIFVGSTYGSEE 360
           HQARNRGLKVRGIIISNPSIPVGNQLNRETLYDLLDFAREKNIHIISNEIFVGSTYGSEE
Sbjct: 301 HQARNRGLKVRGIIISNPSIPVGNQLNRETLYDLLDFAREKNIHIISNEIFVGSTYGSEE 360

Query: 361 FVSMAEIIDAEDSDRDRVHITYALSEDMSLPGFGVAAIYSLNENILAAARKLARFSSVSA 420
           FVSMAE+ID ED+DRDRVHITY LSEDMSLPGF VAAIYSLNENILAAARKLARFSSVSA
Sbjct: 361 FVSMAEMIDLEDNDRDRVHITYGLSEDMSLPGFRVAAIYSLNENILAAARKLARFSSVSA 420

Query: 421 ITQNLLSSMLSDTQFIQRFIRTNRERLGEMDGKFTAGLKELNIKCMRSNGGFYCWADMSK 480
           ITQNLLSSMLSDTQFIQ FIRTNRERLGEM+ KFTAGLKEL+IKC++SNGGFYCWADMSK
Sbjct: 421 ITQNLLSSMLSDTQFIQSFIRTNRERLGEMNSKFTAGLKELDIKCVKSNGGFYCWADMSK 480

Query: 481 LIRSYSEKGELELWEKLVDIGKVNVIPGSSCNCIEPGWFGFCFTTLIDKEIPIVMDRIRR 540
           LIRSYSEKGELELWEKL D+GKVNV PGS CNCIEPGWFGFCFTT +DKEIPIVMDRIRR
Sbjct: 481 LIRSYSEKGELELWEKLFDVGKVNVTPGSCCNCIEPGWFGFCFTTSVDKEIPIVMDRIRR 540

Query: 541 ISEGCKSYT 550
           ISE CKSYT
Sbjct: 541 ISEACKSYT 548

BLAST of Bhi12G000329 vs. ExPASy TrEMBL
Match: A0A2H4KKM8 (1-aminocyclopropane-1-carboxylic acid synthase OS=Siraitia grosvenorii OX=190515 GN=ACS10 PE=2 SV=1)

HSP 1 Score: 974.2 bits (2517), Expect = 2.3e-280
Identity = 489/549 (89.07%), Postives = 516/549 (93.99%), Query Frame = 0

Query: 1   MTQTRFFRTRNSKSDKEAEPTTSGGGGAAMRVIVPLQGVVQGRGGVVLGSVIPCVLFYFL 60
           MTQTRFFRTRN KSD+EAEPTTSGGGGAAMRVIVPLQGVVQGRGGVVLGSVIPCVLFYFL
Sbjct: 1   MTQTRFFRTRNPKSDEEAEPTTSGGGGAAMRVIVPLQGVVQGRGGVVLGSVIPCVLFYFL 60

Query: 61  QLYLKRNRSEPSPDPPSSQEVDSSGNSSPSSSNQLVELSVLPRTLSRTFLSPRSSGPLCV 120
           QLYLKRNRS+P+ DPP S EV S GNSSP+S N LVELSVLPRTLSRTFLSPRSSGP+CV
Sbjct: 61  QLYLKRNRSDPNRDPPPSPEVASPGNSSPTSGNPLVELSVLPRTLSRTFLSPRSSGPVCV 120

Query: 121 SGRANSISRVGDSTIFVGMRRFLEDPFDELDNPTGIIQLSVAENKLSSDLVQNWLTENGR 180
           SGRANSI+R GDS+ F+GMR+FLEDP+DELDNPTGIIQL VAENKLSSDLVQNWLTENGR
Sbjct: 121 SGRANSIARGGDSSFFLGMRKFLEDPYDELDNPTGIIQLCVAENKLSSDLVQNWLTENGR 180

Query: 181 DAILGGVNHNELSVAGTVSYRPSDGLTDLKLAVASFMSQVVGNSVSFIPSHLVFTTGVTS 240
           DAILGG NHN LSVAGT SY+P DGLTDLKLAVA FMSQ VGNSVSF PS LVFTTGVTS
Sbjct: 181 DAILGG-NHNGLSVAGTASYQPMDGLTDLKLAVAGFMSQAVGNSVSFSPSQLVFTTGVTS 240

Query: 241 AIETLCFCIADPGNAFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSITSLDRAF 300
           AIETLCFCIADPG+AFLVP+PYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSI SLDRAF
Sbjct: 241 AIETLCFCIADPGHAFLVPSPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSINSLDRAF 300

Query: 301 HQARNRGLKVRGIIISNPSIPVGNQLNRETLYDLLDFAREKNIHIISNEIFVGSTYGSEE 360
           HQA+ RGLKVRGIIISNPSIPVGNQL+RETLY++LDFAREKNIHIISNEIFVGSTYGSEE
Sbjct: 301 HQAKKRGLKVRGIIISNPSIPVGNQLHRETLYNILDFAREKNIHIISNEIFVGSTYGSEE 360

Query: 361 FVSMAEIIDAEDSDRDRVHITYALSEDMSLPGFGVAAIYSLNENILAAARKLARFSSVSA 420
           FVSMAE+ID EDSDRDRVHI Y LSED+SLPGF VAAIYSLNENILAAA+KLARFSSVSA
Sbjct: 361 FVSMAEMIDLEDSDRDRVHIAYGLSEDLSLPGFRVAAIYSLNENILAAAKKLARFSSVSA 420

Query: 421 ITQNLLSSMLSDTQFIQRFIRTNRERLGEMDGKFTAGLKELNIKCMRSNGGFYCWADMSK 480
           ITQNLLSSML DT F+QRFIR NRERLGEM GKF AGLKEL+IKCM+SNGGFYCWADMSK
Sbjct: 421 ITQNLLSSMLLDTVFVQRFIRINRERLGEMHGKFVAGLKELDIKCMKSNGGFYCWADMSK 480

Query: 481 LIRSYSEKGELELWEKLVDIGKVNVIPGSSCNCIEPGWFGFCFTTLIDKEIPIVMDRIRR 540
           LIRSYSEKGELELW+KL+DIGKVNV PGSSCNCIE GWFGFCFTTLID+EIPIVMDRIRR
Sbjct: 481 LIRSYSEKGELELWDKLLDIGKVNVAPGSSCNCIELGWFGFCFTTLIDREIPIVMDRIRR 540

Query: 541 ISEGCKSYT 550
           ISE CKSY+
Sbjct: 541 ISEACKSYS 548

BLAST of Bhi12G000329 vs. ExPASy TrEMBL
Match: A0A6J1H0W9 (probable aminotransferase ACS10 OS=Cucurbita moschata OX=3662 GN=LOC111459137 PE=4 SV=1)

HSP 1 Score: 955.3 bits (2468), Expect = 1.1e-274
Identity = 480/549 (87.43%), Postives = 510/549 (92.90%), Query Frame = 0

Query: 1   MTQTRFFRTRNSKSDKEAEPTTSGGGGAAMRVIVPLQGVVQGRGGVVLGSVIPCVLFYFL 60
           MT TRFFR RNSKS+KE EPTTS GGG AMRVIVPLQG+VQG GGVVLGS+IPCVLFYFL
Sbjct: 1   MTHTRFFRNRNSKSNKEGEPTTSDGGGTAMRVIVPLQGIVQGSGGVVLGSLIPCVLFYFL 60

Query: 61  QLYLKRNRSEPSPDPPSSQEVDSSGNSSPSSSNQLVELSVLPRTLSRTFLSPRSSGPLCV 120
           QLYLKRNRSEP+ D PSS EVDS GNSS  SSNQ VELS LPRTLSRTFLS RS+GP+CV
Sbjct: 61  QLYLKRNRSEPTEDSPSSPEVDSPGNSSSPSSNQSVELSSLPRTLSRTFLSSRSNGPVCV 120

Query: 121 SGRANSISRVGDSTIFVGMRRFLEDPFDELDNPTGIIQLSVAENKLSSDLVQNWLTENGR 180
           SGRANSI++VG+S+IFVGM++F EDPFDELDNPTGIIQLS+AENKLS+DLVQNWL ENGR
Sbjct: 121 SGRANSIAKVGESSIFVGMKKFFEDPFDELDNPTGIIQLSIAENKLSTDLVQNWLVENGR 180

Query: 181 DAILGGVNHNELSVAGTVSYRPSDGLTDLKLAVASFMSQVVGNSVSFIPSHLVFTTGVTS 240
           DAILG  NHNELSVAGTVSY+PSDGL DLKLAVASFMSQ VGNSVSFIPS LVFT GV+S
Sbjct: 181 DAILGVGNHNELSVAGTVSYQPSDGLMDLKLAVASFMSQAVGNSVSFIPSQLVFTNGVSS 240

Query: 241 AIETLCFCIADPGNAFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSITSLDRAF 300
           AIETLCFCIAD G+AFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSITSLDRAF
Sbjct: 241 AIETLCFCIADAGHAFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSITSLDRAF 300

Query: 301 HQARNRGLKVRGIIISNPSIPVGNQLNRETLYDLLDFAREKNIHIISNEIFVGSTYGSEE 360
           HQAR+RGLKVRGIIISNPSIPVGNQL++ETLY LLDFAR+KNIHIISNEIFVGSTYGSEE
Sbjct: 301 HQARSRGLKVRGIIISNPSIPVGNQLHKETLYKLLDFARDKNIHIISNEIFVGSTYGSEE 360

Query: 361 FVSMAEIIDAEDSDRDRVHITYALSEDMSLPGFGVAAIYSLNENILAAARKLARFSSVSA 420
           FVSMAE+ID EDSDRDRVHITY LSEDMSLPGF VAAIYSLNENILAAA KLARFSSVSA
Sbjct: 361 FVSMAEMIDLEDSDRDRVHITYGLSEDMSLPGFRVAAIYSLNENILAAAGKLARFSSVSA 420

Query: 421 ITQNLLSSMLSDTQFIQRFIRTNRERLGEMDGKFTAGLKELNIKCMRSNGGFYCWADMSK 480
           ITQNLLSSMLSD QFIQ+FI  NR+RLGEM+ KF AGLKEL+IK MRSNGGFYCWADM  
Sbjct: 421 ITQNLLSSMLSDAQFIQKFIGINRKRLGEMNSKFVAGLKELDIKYMRSNGGFYCWADMGN 480

Query: 481 LIRSYSEKGELELWEKLVDIGKVNVIPGSSCNCIEPGWFGFCFTTLIDKEIPIVMDRIRR 540
           LIRSYSEKGE+ELW+KL+DIGKV+VIPGS CNCIEPGWFGFCFTTLIDKEIPIVMDRIRR
Sbjct: 481 LIRSYSEKGEVELWDKLLDIGKVHVIPGSCCNCIEPGWFGFCFTTLIDKEIPIVMDRIRR 540

Query: 541 ISEGCKSYT 550
           ISE CKSYT
Sbjct: 541 ISEACKSYT 549

BLAST of Bhi12G000329 vs. ExPASy TrEMBL
Match: A0A6J1K1G9 (probable aminotransferase ACS10 OS=Cucurbita maxima OX=3661 GN=LOC111491555 PE=4 SV=1)

HSP 1 Score: 953.0 bits (2462), Expect = 5.5e-274
Identity = 480/549 (87.43%), Postives = 509/549 (92.71%), Query Frame = 0

Query: 1   MTQTRFFRTRNSKSDKEAEPTTSGGGGAAMRVIVPLQGVVQGRGGVVLGSVIPCVLFYFL 60
           MT TRFFR RNSKS+KE EPTTS GGG AMRVIVPLQG+VQG GGVVLGS+IPCVLFYFL
Sbjct: 1   MTHTRFFRNRNSKSNKEGEPTTSDGGGTAMRVIVPLQGIVQGSGGVVLGSLIPCVLFYFL 60

Query: 61  QLYLKRNRSEPSPDPPSSQEVDSSGNSSPSSSNQLVELSVLPRTLSRTFLSPRSSGPLCV 120
           QLYLKRNRSEP+ DPPSS EVDS GNSS  SSNQ VELS LPRTLSRTFLS RS+GP+CV
Sbjct: 61  QLYLKRNRSEPTEDPPSSPEVDSPGNSSSPSSNQSVELSPLPRTLSRTFLSSRSNGPVCV 120

Query: 121 SGRANSISRVGDSTIFVGMRRFLEDPFDELDNPTGIIQLSVAENKLSSDLVQNWLTENGR 180
           S RANSI++VG+S+IFVGM++F EDPFDELDNPTGIIQLS+AENKLSSDLVQNWL ENGR
Sbjct: 121 SCRANSIAKVGESSIFVGMKKFFEDPFDELDNPTGIIQLSIAENKLSSDLVQNWLEENGR 180

Query: 181 DAILGGVNHNELSVAGTVSYRPSDGLTDLKLAVASFMSQVVGNSVSFIPSHLVFTTGVTS 240
           DAILGG NHNELSVAGTVSY+PSDGL DLKLAVASFMSQ VGNSVSFIPS LVFT GV+S
Sbjct: 181 DAILGGGNHNELSVAGTVSYQPSDGLMDLKLAVASFMSQAVGNSVSFIPSQLVFTNGVSS 240

Query: 241 AIETLCFCIADPGNAFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSITSLDRAF 300
           AIETL FCIAD G+AFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSITSLDRAF
Sbjct: 241 AIETLSFCIADAGHAFLVPTPYYPGLDNDVKWRTGVEIVPVPCRSADNFSLSITSLDRAF 300

Query: 301 HQARNRGLKVRGIIISNPSIPVGNQLNRETLYDLLDFAREKNIHIISNEIFVGSTYGSEE 360
           HQAR+RGLKVRGIIISNPSIPVGNQL++ETLY LLDFAR+KNIHIISNEIFVGSTYGSEE
Sbjct: 301 HQARSRGLKVRGIIISNPSIPVGNQLHKETLYKLLDFARDKNIHIISNEIFVGSTYGSEE 360

Query: 361 FVSMAEIIDAEDSDRDRVHITYALSEDMSLPGFGVAAIYSLNENILAAARKLARFSSVSA 420
           FVSMAE+ID EDSDRDRVHITY LSEDMSLPGF VAAIYSLNENILAAA KLARFSSVSA
Sbjct: 361 FVSMAEMIDLEDSDRDRVHITYGLSEDMSLPGFRVAAIYSLNENILAAAGKLARFSSVSA 420

Query: 421 ITQNLLSSMLSDTQFIQRFIRTNRERLGEMDGKFTAGLKELNIKCMRSNGGFYCWADMSK 480
           ITQNLLSSMLSD QFIQ+FI  NR+RLGEM+ KF AGLKEL+IK MRSNGGFYCWADM  
Sbjct: 421 ITQNLLSSMLSDAQFIQKFIGINRKRLGEMNSKFVAGLKELDIKYMRSNGGFYCWADMGN 480

Query: 481 LIRSYSEKGELELWEKLVDIGKVNVIPGSSCNCIEPGWFGFCFTTLIDKEIPIVMDRIRR 540
           LIRSYSEKGE+ELW+KL+DIGKV+VIPGS CNCIEPGWFGFCFTTLIDKEIPIVMDRIR 
Sbjct: 481 LIRSYSEKGEVELWDKLLDIGKVHVIPGSCCNCIEPGWFGFCFTTLIDKEIPIVMDRIRT 540

Query: 541 ISEGCKSYT 550
           ISE CKSYT
Sbjct: 541 ISEACKSYT 549

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT1G62960.13.9e-17958.26ACC synthase 10 [more]
AT5G51690.11.2e-15651.731-amino-cyclopropane-1-carboxylate synthase 12 [more]
AT4G37770.16.8e-10743.981-amino-cyclopropane-1-carboxylate synthase 8 [more]
AT3G49700.11.5e-10643.191-aminocyclopropane-1-carboxylate synthase 9 [more]
AT5G65800.19.8e-10642.42ACC synthase 5 [more]
Match NameE-valueIdentityDescription
Q9LQ105.5e-17858.26Probable aminotransferase ACS10 OS=Arabidopsis thaliana OX=3702 GN=ACS10 PE=1 SV... [more]
Q8GYY01.7e-15551.73Probable aminotransferase ACS12 OS=Arabidopsis thaliana OX=3702 GN=ACS12 PE=2 SV... [more]
P378214.3e-10642.821-aminocyclopropane-1-carboxylate synthase OS=Malus domestica OX=3750 GN=ACS-1 P... [more]
Q9T0659.6e-10643.981-aminocyclopropane-1-carboxylate synthase 8 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9M2Y82.1e-10543.191-aminocyclopropane-1-carboxylate synthase 9 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Match NameE-valueIdentityDescription
XP_038874650.10.0e+00100.00probable aminotransferase ACS10 [Benincasa hispida][more]
XP_004147561.11.1e-29293.81probable aminotransferase ACS10 [Cucumis sativus] >KGN50179.1 hypothetical prote... [more]
XP_008437168.17.1e-29293.08PREDICTED: probable aminotransferase ACS10 [Cucumis melo][more]
ASV63085.14.8e-28089.071-aminocyclopropane-1-carboxylic acid synthase [Siraitia grosvenorii][more]
XP_023532648.11.0e-27487.43probable aminotransferase ACS10 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A0A0KMR45.3e-29393.81Aminotran_1_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G1573... [more]
A0A1S3ATY53.5e-29293.08probable aminotransferase ACS10 OS=Cucumis melo OX=3656 GN=LOC103482676 PE=4 SV=... [more]
A0A2H4KKM82.3e-28089.071-aminocyclopropane-1-carboxylic acid synthase OS=Siraitia grosvenorii OX=190515... [more]
A0A6J1H0W91.1e-27487.43probable aminotransferase ACS10 OS=Cucurbita moschata OX=3662 GN=LOC111459137 PE... [more]
A0A6J1K1G95.5e-27487.43probable aminotransferase ACS10 OS=Cucurbita maxima OX=3661 GN=LOC111491555 PE=4... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00753ACCSYNTHASEcoord: 409..432
score: 54.71
coord: 377..401
score: 58.62
coord: 307..331
score: 44.52
coord: 343..366
score: 39.96
coord: 231..251
score: 50.66
coord: 159..185
score: 37.28
coord: 253..274
score: 71.24
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 71..95
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..28
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 68..95
NoneNo IPR availablePANTHERPTHR43795BIFUNCTIONAL ASPARTATE AMINOTRANSFERASE AND GLUTAMATE/ASPARTATE-PREPHENATE AMINOTRANSFERASE-RELATEDcoord: 21..544
NoneNo IPR availablePANTHERPTHR43795:SF46AMINOTRANSFERASE ACS12-RELATEDcoord: 21..544
NoneNo IPR availableCDDcd00609AAT_likecoord: 195..540
e-value: 5.07601E-47
score: 165.977
IPR015421Pyridoxal phosphate-dependent transferase, major domainGENE3D3.40.640.10coord: 179..432
e-value: 1.1E-130
score: 437.9
IPR015422Pyridoxal phosphate-dependent transferase, small domainGENE3D3.90.1150.10Aspartate Aminotransferase, domain 1coord: 145..537
e-value: 1.1E-130
score: 437.9
IPR004839Aminotransferase, class I/classIIPFAMPF00155Aminotran_1_2coord: 164..538
e-value: 1.0E-67
score: 228.9
IPR015424Pyridoxal phosphate-dependent transferaseSUPERFAMILY53383PLP-dependent transferasescoord: 128..541

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi12M000329Bhi12M000329mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009058 biosynthetic process
biological_process GO:0006520 cellular amino acid metabolic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005739 mitochondrion
molecular_function GO:0008793 aromatic-amino-acid:2-oxoglutarate aminotransferase activity
molecular_function GO:0004069 L-aspartate:2-oxoglutarate aminotransferase activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0003824 catalytic activity