Bhi12G000322 (gene) Wax gourd (B227) v1

BLAST of Bhi12G000322 vs. TAIR 10
Match: AT1G04110.1 (Subtilase family protein )

HSP 1 Score: 620.2 bits (1598), Expect = 2.2e-177
Identity = 369/789 (46.77%), Postives = 492/789 (62.36%), Query Frame = 0

Query: 4   PFF-CIFFLLNFHGIVAMKSELSGTSNQIKLQTYIV--HVKESELRILGDAIDLESWYTS 63
           PFF CI FLL            S +S  ++ QTYIV  H      +      D   W+ S
Sbjct: 5   PFFLCIIFLL----------FCSSSSEILQKQTYIVQLHPNSETAKTFASKFD---WHLS 64

Query: 64  FLPETIKTSNEQ-----SRLLYSFRNVMSGFSARLTE-EVKAMEEKEGFISARPETILNL 123
           FL E +    E+     SRLLYS+ + + GF+A+LTE E + +      ++ RP+ +L +
Sbjct: 65  FLQEAVLGVEEEEEEPSSRLLYSYGSAIEGFAAQLTESEAEILRYSPEVVAVRPDHVLQV 124

Query: 124 HTTHTPEYLGLN--QQFGLWKDSNFGKGVIIGVLDTGISPDHPSFNDDGMPQPPAKWKGR 183
            TT++ ++LGL+     G+W  S FG+G IIGVLDTG+ P+ PSF+D GMP  P KWKG 
Sbjct: 125 QTTYSYKFLGLDGFGNSGVWSKSRFGQGTIIGVLDTGVWPESPSFDDTGMPSIPRKWKGI 184

Query: 184 CE----FGASICNNKLIGARTFNRANSALMG--ESP---------IDENGHGTHTASTAA 243
           C+    F +S CN KLIGAR F R +       ESP          D  GHGTHTAST  
Sbjct: 185 CQEGESFSSSSCNRKLIGARFFIRGHRVANSPEESPNMPREYISARDSTGHGTHTASTVG 244

Query: 244 GTFVEGAEALGNARGKAVGMAPLAHLAMYKVCSPKGCSSSDILAALDAAIHDGVDVLSLS 303
           G+ V  A  LGN  G A GMAP AH+A+YKVC   GC SSDILAA+D AI D VDVLSLS
Sbjct: 245 GSSVSMANVLGNGAGVARGMAPGAHIAVYKVCWFNGCYSSDILAAIDVAIQDKVDVLSLS 304

Query: 304 LGAPSRPFYIDNIAIGAFAAIKMGIFVSCSAGNSGPSKSTLANEAPWILTVGASTIDRTI 363
           LG    P Y D IAIG F A++ GI V C+AGN+GP +S++AN APW+ T+GA T+DR  
Sbjct: 305 LGGFPIPLYDDTIAIGTFRAMERGISVICAAGNNGPIESSVANTAPWVSTIGAGTLDRRF 364

Query: 364 MALARLGNGEVHVGESLFQPRDFPSS--FLPLVYAGSSGVKGSEFCVQGSLTNLNVKGKM 423
            A+ RL NG++  GESL+  +   ++   + ++Y  + G KGSEFC++GSL    ++GKM
Sbjct: 365 PAVVRLANGKLLYGESLYPGKGIKNAGREVEVIYV-TGGDKGSEFCLRGSLPREEIRGKM 424

Query: 424 VVCERGGGIGRIAKGMVVKNAGGAAMIIINQKADEFSTLAEAHILPATHVSYEAGLKIKS 483
           V+C+RG   GR  KG  VK AGG AMI+ N + ++     + H+LPAT + Y   + +K+
Sbjct: 425 VICDRGVN-GRSEKGEAVKEAGGVAMILANTEINQEEDSIDVHLLPATLIGYTESVLLKA 484

Query: 484 YIRSSQNPMASISFEGTLIG-SRATTFSPAMASFSSRGPCLPSPGILKPDITGPGVNILA 543
           Y+ ++  P A I F GT+IG SRA    P +A FS+RGP L +P ILKPD+  PGVNI+A
Sbjct: 485 YVNATVKPKARIIFGGTVIGRSRA----PEVAQFSARGPSLANPSILKPDMIAPGVNIIA 544

Query: 544 AWPFSLDNN----TNKKSTFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTSA 603
           AWP +L        +++  F V+SGTSMSCPH+SGI ALI+S +PNWSPAAIKSA+MT+A
Sbjct: 545 AWPQNLGPTGLPYDSRRVNFTVMSGTSMSCPHVSGITALIRSAYPNWSPAAIKSALMTTA 604

Query: 604 DVRNRQGRPIVDQDLKPANFFAMGSGHVNPSKAADPGLVYDIQPDDYIPYLCGL-YSDAQ 663
           D+ +RQG+ I D + KPA  FA+G+GHVNP KA +PGLVY+IQP DYI YLC L ++ + 
Sbjct: 605 DLYDRQGKAIKDGN-KPAGVFAIGAGHVNPQKAINPGLVYNIQPVDYITYLCTLGFTRSD 664

Query: 664 VSIIVRRKVTCSTISRIREG-DLNYPSFAVTL---GPSQMFNRTVTNVGDANSIYSAIVE 723
           +  I  + V+C+ I R   G  LNYPS AV       ++M  R VTNVG  NSIYS  V+
Sbjct: 665 ILAITHKNVSCNGILRKNPGFSLNYPSIAVIFKRGKTTEMITRRVTNVGSPNSIYSVNVK 724

Query: 724 APIGVSVKVTPNNLKFSRVIEKVTYSVTF--SRDDLVRTTSEFSEGYLIWVSNKHM---V 750
           AP G+ V V P  L F  V + ++Y V F   + +     + F++G L WV++ ++   V
Sbjct: 725 APEGIKVIVNPKRLVFKHVDQTLSYRVWFVLKKKNRGGKVASFAQGQLTWVNSHNLMQRV 773

BLAST of Bhi12G000322 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 607.1 bits (1564), Expect = 2.0e-173
Identity = 353/763 (46.26%), Postives = 477/763 (62.52%), Query Frame = 0

Query: 9   FFLLNFHGIVAMKSELSGTSNQIKLQTYIVHVKESELRILGDAIDLES-WYTSFLPETIK 68
           FFLL   G   + S  S         TYIVH+ +S+   +  + DL S WY S    +++
Sbjct: 11  FFLLLCLGFCHVSSSSSDQG------TYIVHMAKSQ---MPSSFDLHSNWYDS----SLR 70

Query: 69  TSNEQSRLLYSFRNVMSGFSARLT-EEVKAMEEKEGFISARPETILNLHTTHTPEYLGLN 128
           + ++ + LLY++ N + GFS RLT EE  ++  + G IS  PE    LHTT TP +LGL+
Sbjct: 71  SISDSAELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLD 130

Query: 129 QQFG-LWKDSNFGKGVIIGVLDTGISPDHPSFNDDGMPQPPAKWKGRCE----FGASICN 188
           +    L+ ++     V++GVLDTG+ P+  S++D+G    P+ WKG CE    F AS+CN
Sbjct: 131 EHTADLFPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCN 190

Query: 189 NKLIGARTFNRANSALMG--------ESPIDENGHGTHTASTAAGTFVEGAEALGNARGK 248
            KLIGAR F R   + MG         SP D++GHGTHT+STAAG+ VEGA  LG A G 
Sbjct: 191 RKLIGARFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGT 250

Query: 249 AVGMAPLAHLAMYKVCSPKGCSSSDILAALDAAIHDGVDVLSLSLGAPSRPFYIDNIAIG 308
           A GMAP A +A+YKVC   GC SSDILAA+D AI D V+VLS+SLG     +Y D +AIG
Sbjct: 251 ARGMAPRARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIG 310

Query: 309 AFAAIKMGIFVSCSAGNSGPSKSTLANEAPWILTVGASTIDRTIMALARLGNGEVHVGES 368
           AFAA++ GI VSCSAGN+GPS S+L+N APWI TVGA T+DR   ALA LGNG+   G S
Sbjct: 311 AFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVS 370

Query: 369 LFQPRDFPSSFLPLVYAG-SSGVKGSEFCVQGSLTNLNVKGKMVVCERGGGIGRIAKGMV 428
           LF+    P   LP +YAG +S       C+ G+L    VKGK+V+C+RG    R+ KG V
Sbjct: 371 LFKGEALPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGIN-ARVQKGDV 430

Query: 429 VKNAGGAAMIIINQKADEFSTLAEAHILPATHVSYEAGLKIKSYIRSSQNPMASISFEGT 488
           VK AGG  MI+ N  A+    +A+AH+LPAT V  +AG  I+ Y+ +  NP ASIS  GT
Sbjct: 431 VKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGT 490

Query: 489 LIGSRATTFSPAMASFSSRGPCLPSPGILKPDITGPGVNILAAW-----PFSLDNNTNKK 548
           ++G +    SP +A+FSSRGP   +P ILKPD+  PGVNILAAW     P  L ++ +++
Sbjct: 491 VVGVKP---SPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWTGAAGPTGLASD-SRR 550

Query: 549 STFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTSADVRNRQGRPIVD-QDLK 608
             FN+ISGTSMSCPH+SG+AAL+KSVHP WSPAAI+SA+MT+A    + G+P++D    K
Sbjct: 551 VEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKTYKDGKPLLDIATGK 610

Query: 609 PANFFAMGSGHVNPSKAADPGLVYDIQPDDYIPYLCGL-YSDAQVSIIVRRKVTCSTISR 668
           P+  F  G+GHV+P+ A +PGL+YD+  +DY+ +LC L Y+  Q+  + RR  TC     
Sbjct: 611 PSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKS 670

Query: 669 IREGDLNYPSFAVTLG--PSQMFNRTVTNVGDANSIYSAIVEAPIGVSVKVTPNNLKFSR 728
               DLNYPSFAV +    +  + RTVT+VG A +    +     GV + V P  L F  
Sbjct: 671 YSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKE 730

Query: 729 VIEKVTYSVTFSRDDLVRTTSEFSEGYLIWVSNKHMVRSPISV 747
             EK +Y+VTF+ D   + +   S G + W   KH+V SP+++
Sbjct: 731 ANEKKSYTVTFTVDS-SKPSGSNSFGSIEWSDGKHVVGSPVAI 754

BLAST of Bhi12G000322 vs. TAIR 10
Match: AT1G01900.1 (subtilase family protein )

HSP 1 Score: 589.7 bits (1519), Expect = 3.2e-168
Identity = 340/772 (44.04%), Postives = 476/772 (61.66%), Query Frame = 0

Query: 5   FFCIFFLLNFHGIVAMKSELSGTSNQIKLQTYIVHVKESELRILGDAIDLESWYTSFLPE 64
           F   FFL+ F   V+ +            QTY++H   +  + +     + S + S   E
Sbjct: 24  FIVFFFLIFFASNVSSRK-----------QTYVIHTVTTSTKHI-----VTSLFNSLQTE 83

Query: 65  TIKTSN-EQSRLLYSFRNVMSGFSARLT-EEVKAMEEKEGFISARPETILNLHTTHTPEY 124
            I   +     + Y + N MSGFSA LT +++  ++  +GFISA P+ +L+LHTT++ E+
Sbjct: 84  NINDDDFSLPEIHYIYENAMSGFSATLTDDQLDTVKNTKGFISAYPDELLSLHTTYSHEF 143

Query: 125 LGLNQQFGLWKDSNFGKGVIIGVLDTGISPDHPSFNDDGMPQPPAKWKGRCE----FGAS 184
           LGL    GLW +++    VIIG++DTGISP+H SF D  M   P++W+G C+    F +S
Sbjct: 144 LGLEFGIGLWNETSLSSDVIIGLVDTGISPEHVSFRDTHMTPVPSRWRGSCDEGTNFSSS 203

Query: 185 ICNNKLIGARTFNRANSALMGE--------SPIDENGHGTHTASTAAGTFVEGAEALGNA 244
            CN K+IGA  F +   +++G+        S  D  GHGTHTASTAAG  V  A   G A
Sbjct: 204 ECNKKIIGASAFYKGYESIVGKINETTDFRSTRDAQGHGTHTASTAAGDIVPKANYFGQA 263

Query: 245 RGKAVGMAPLAHLAMYKVCSPKGCSSSDILAALDAAIHDGVDVLSLSLGAPSRPFYIDNI 304
           +G A GM   + +A YK C   GC+S+D++AA+D AI DGVDV+SLSLG  SRPFY+D I
Sbjct: 264 KGLASGMRFTSRIAAYKACWALGCASTDVIAAIDRAILDGVDVISLSLGGSSRPFYVDPI 323

Query: 305 AIGAFAAIKMGIFVSCSAGNSGPSKSTLANEAPWILTVGASTIDRTIMALARLGNGEVHV 364
           AI  F A++  IFVSCSAGNSGP+ ST++N APW++TV AS  DRT  A+ R+GN +  V
Sbjct: 324 AIAGFGAMQKNIFVSCSAGNSGPTASTVSNGAPWLMTVAASYTDRTFPAIVRIGNRKSLV 383

Query: 365 GESLFQPRDFPSSFLPLVYAGSSGVK-GSEFCVQGSLTNLNVKGKMVVCERGGGIGRIAK 424
           G SL++ +   +  LPL +  ++G + G+ FC++ SL    V+GK+V+C RG   GR AK
Sbjct: 384 GSSLYKGKSLKN--LPLAFNRTAGEESGAVFCIRDSLKRELVEGKIVICLRGAS-GRTAK 443

Query: 425 GMVVKNAGGAAMIIINQKADEFSTLAEAHILPATHVSYEAGLKIKSYIRSSQNPMASISF 484
           G  VK +GGAAM++++ +A+    LA+ H+LPA  + +  G  + +Y+  + N  AS+ F
Sbjct: 444 GEEVKRSGGAAMLLVSTEAEGEELLADPHVLPAVSLGFSDGKTLLNYLAGAANATASVRF 503

Query: 485 EGTLIGSRATTFSPAMASFSSRGPCLPSPGILKPDITGPGVNILAAW-PF---SLDNNTN 544
            GT  G+ A    P +A+FSSRGP +  P I KPDI  PG+NILA W PF   SL  +  
Sbjct: 504 RGTAYGATA----PMVAAFSSRGPSVAGPEIAKPDIAAPGLNILAGWSPFSSPSLLRSDP 563

Query: 545 KKSTFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTSADVRNRQGRPIVDQDL 604
           ++  FN+ISGTSM+CPH+SGIAALIKSVH +WSPA IKSAIMT+A + + + RPI D+  
Sbjct: 564 RRVQFNIISGTSMACPHISGIAALIKSVHGDWSPAMIKSAIMTTARITDNRNRPIGDRGA 623

Query: 605 ----KPANFFAMGSGHVNPSKAADPGLVYDIQPDDYIPYLCGL-YSDAQVSIIVRRKVTC 664
                 A  FA G+G+V+P++A DPGLVYD    DY+ YLC L Y+  ++ +      TC
Sbjct: 624 AGAESAATAFAFGAGNVDPTRAVDPGLVYDTSTVDYLNYLCSLNYTSERILLFSGTNYTC 683

Query: 665 -STISRIREGDLNYPSFAVTLG-----PSQMFNRTVTNVGDANSIYSAIVEAPIGVSVKV 724
            S    +  GDLNYPSFAV L       +  + RTVTNVG     Y   VE P GV V+V
Sbjct: 684 ASNAVVLSPGDLNYPSFAVNLVNGANLKTVRYKRTVTNVGSPTCEYMVHVEEPKGVKVRV 743

Query: 725 TPNNLKFSRVIEKVTYSVTFSRDDLVRTTSEFSEGYLIWVSNKHMVRSPISV 747
            P  LKF +  E+++Y+VT+   +  R +S  S G L+W+ +K+ VRSPI+V
Sbjct: 744 EPKVLKFQKARERLSYTVTYDA-EASRNSSSSSFGVLVWICDKYNVRSPIAV 771

BLAST of Bhi12G000322 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 587.4 bits (1513), Expect = 1.6e-167
Identity = 348/756 (46.03%), Postives = 467/756 (61.77%), Query Frame = 0

Query: 27  TSNQIKLQTYIVHVKESELRILGDAIDLESWYTSFLPETIKTSNEQSRLLYSFRNVMSGF 86
           +S+   L++YIVHV+ S    L  + +  +W+ S L  ++ +S + + LLYS+   + GF
Sbjct: 24  SSSSDGLESYIVHVQRSHKPSLFSSHN--NWHVSLL-RSLPSSPQPATLLYSYSRAVHGF 83

Query: 87  SARLTE-EVKAMEEKEGFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNFGKGVIIGV 146
           SARL+  +  A+      IS  P+    +HTTHTP +LG +Q  GLW +SN+G+ VI+GV
Sbjct: 84  SARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSNSNYGEDVIVGV 143

Query: 147 LDTGISPDHPSFNDDGMPQPPAKWKGRCEFG----ASICNNKLIGARTFNRA-------- 206
           LDTGI P+HPSF+D G+   P+ WKG CE G    AS CN KLIGAR F R         
Sbjct: 144 LDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARAFYRGYLTQRNGT 203

Query: 207 --NSALMGESPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGMAPLAHLAMYKVCSP 266
             ++A    SP D  GHGTHTASTAAG+ V  A     ARG A GMA  A +A YK+C  
Sbjct: 204 KKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIAAYKICWT 263

Query: 267 KGCSSSDILAALDAAIHDGVDVLSLSLGAP-SRP-FYIDNIAIGAFAAIKMGIFVSCSAG 326
            GC  SDILAA+D A+ DGV V+SLS+GA  S P ++ D+IAIGAF A + GI VSCSAG
Sbjct: 264 GGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATRHGIVVSCSAG 323

Query: 327 NSGPSKSTLANEAPWILTVGASTIDRTIMALARLGNGEVHVGESLFQPRDFPSSFLPLVY 386
           NSGP+  T  N APWILTVGAST+DR   A A  G+G+V  G SL+     P S L LVY
Sbjct: 324 NSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGESLPDSQLSLVY 383

Query: 387 AGSSGVKGSEFCVQGSLTNLNVKGKMVVCERGGGIGRIAKGMVVKNAGGAAMIIINQKAD 446
           +G     GS  C  G L +  V+GK+V+C+RGG   R+ KG  VK AGGA MI+ N    
Sbjct: 384 SGDC---GSRLCYPGKLNSSLVEGKIVLCDRGGN-ARVEKGSAVKLAGGAGMILANTAES 443

Query: 447 EFSTLAEAHILPATHVSYEAGLKIKSYIRSSQNPMASISFEGTLIGSRATTFSPAMASFS 506
                A++H++PAT V  +AG +I+ YI++S +P A ISF GTLIG      SP +A+FS
Sbjct: 444 GEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSPP--SPRVAAFS 503

Query: 507 SRGPCLPSPGILKPDITGPGVNILAAW-----PFSLDNNTNKKSTFNVISGTSMSCPHLS 566
           SRGP   +P ILKPD+  PGVNILA W     P  LD +  ++  FN+ISGTSMSCPH+S
Sbjct: 504 SRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDP-RRVQFNIISGTSMSCPHVS 563

Query: 567 GIAALIKSVHPNWSPAAIKSAIMTSA-DVRNRQGRPIVDQDL-KPANFFAMGSGHVNPSK 626
           G+AAL++  HP+WSPAAIKSA++T+A DV N  G PI D    K +N F  G+GHV+P+K
Sbjct: 564 GLAALLRKAHPDWSPAAIKSALVTTAYDVEN-SGEPIEDLATGKSSNSFIHGAGHVDPNK 623

Query: 627 AADPGLVYDIQPDDYIPYLCGLYSDAQVSIIVRRKVT----CSTISRIREGDLNYPSFAV 686
           A +PGLVYDI+  +Y+ +LC +  +    ++  +  T    C T      GDLNYPSF+V
Sbjct: 624 ALNPGLVYDIEVKEYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYPSFSV 683

Query: 687 ---TLGPSQMFNRTVTNVG-DANSIYSAIVEAPIGVSVKVTPNNLKFSRVIEKVTYSVTF 746
              + G    + R V NVG + +++Y   V++P  V + V+P+ L FS+    + Y VTF
Sbjct: 684 VFASTGEVVKYKRVVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSVLEYEVTF 743

Query: 747 SRDDL---VRTTSEFSEGYLIWVSNKHMVRSPISVK 748
               L   V +      G + W   +H+V+SP++V+
Sbjct: 744 KSVVLGGGVGSVPGHEFGSIEWTDGEHVVKSPVAVQ 768

BLAST of Bhi12G000322 vs. TAIR 10
Match: AT2G05920.1 (Subtilase family protein )

HSP 1 Score: 582.8 bits (1501), Expect = 4.0e-166
Identity = 337/737 (45.73%), Postives = 453/737 (61.47%), Query Frame = 0

Query: 34  QTYIVHVKESELRILGDAIDLESWYTSFLPETIKTSNEQSRLLYSFRNVMSGFSARL--T 93
           +TYI+ V  S+       +    WYTS L       N +S LLY++     GFSA L  T
Sbjct: 28  KTYIIRVNHSDKP--ESFLTHHDWYTSQL-------NSESSLLYTYTTSFHGFSAYLDST 87

Query: 94  EEVKAMEEKEGFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNFGKGVIIGVLDTGIS 153
           E    +      +    + +  LHTT TPE+LGLN +FG+    +   GVIIGVLDTG+ 
Sbjct: 88  EADSLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVW 147

Query: 154 PDHPSFNDDGMPQPPAKWKGRCE----FGASICNNKLIGARTFNRA---------NSALM 213
           P+  SF+D  MP+ P+KWKG CE    F + +CN KLIGAR+F++          +S   
Sbjct: 148 PESRSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRE 207

Query: 214 GESPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGMAPLAHLAMYKVCSPKGCSSSD 273
             SP D +GHGTHT++TAAG+ V  A  LG A G A GMA  A +A YKVC   GC  SD
Sbjct: 208 SVSPRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSD 267

Query: 274 ILAALDAAIHDGVDVLSLSLGAPSRPFYIDNIAIGAFAAIKMGIFVSCSAGNSGPSKSTL 333
           ILAA+D AI DGVDVLSLSLG  S P+Y D IAIGAF+A++ G+FVSCSAGNSGP+++++
Sbjct: 268 ILAAMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASV 327

Query: 334 ANEAPWILTVGASTIDRTIMALARLGNGEVHVGESLFQPRDFPSSFLPLVYAGSSGVKGS 393
           AN APW++TVGA T+DR   A A LGNG+   G SL+      +  L LVY        S
Sbjct: 328 ANVAPWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVY-NKGNSSSS 387

Query: 394 EFCVQGSLTNLNVKGKMVVCERGGGIGRIAKGMVVKNAGGAAMIIINQKADEFSTLAEAH 453
             C+ GSL +  V+GK+VVC+RG    R+ KG VV++AGG  MI+ N  A     +A++H
Sbjct: 388 NLCLPGSLDSSIVRGKIVVCDRGVN-ARVEKGAVVRDAGGLGMIMANTAASGEELVADSH 447

Query: 454 ILPATHVSYEAGLKIKSYIRSSQNPMASISFEGTLIGSRATTFSPAMASFSSRGPCLPSP 513
           +LPA  V  + G  ++ Y++S   P A + F+GT++  +    SP +A+FSSRGP   +P
Sbjct: 448 LLPAIAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKP---SPVVAAFSSRGPNTVTP 507

Query: 514 GILKPDITGPGVNILAAW-----PFSLDNNTNKKSTFNVISGTSMSCPHLSGIAALIKSV 573
            ILKPD+ GPGVNILA W     P  LD + ++++ FN++SGTSMSCPH+SG+A L+K+ 
Sbjct: 508 EILKPDVIGPGVNILAGWSDAIGPTGLDKD-SRRTQFNIMSGTSMSCPHISGLAGLLKAA 567

Query: 574 HPNWSPAAIKSAIMTSADVRNRQGRPIVD-QDLKPANFFAMGSGHVNPSKAADPGLVYDI 633
           HP WSP+AIKSA+MT+A V +    P+ D  D   +N +A GSGHV+P KA  PGLVYDI
Sbjct: 568 HPEWSPSAIKSALMTTAYVLDNTNAPLHDAADNSLSNPYAHGSGHVDPQKALSPGLVYDI 627

Query: 634 QPDDYIPYLCGL-YS-DAQVSIIVRRKVTCSTISRIREGDLNYPSFAVTLGPSQM--FNR 693
             ++YI +LC L Y+ D  V+I+ R  V CS       G LNYPSF+V  G  ++  + R
Sbjct: 628 STEEYIRFLCSLDYTVDHIVAIVKRPSVNCSK-KFSDPGQLNYPSFSVLFGGKRVVRYTR 687

Query: 694 TVTNVGDANSIYSAIVEAPIGVSVKVTPNNLKFSRVIEKVTYSVTFSRDDLVRTTSEFSE 746
            VTNVG A+S+Y   V     V + V P+ L F  V EK  Y+VTF     V  T++   
Sbjct: 688 EVTNVGAASSVYKVTVNGAPSVGISVKPSKLSFKSVGEKKRYTVTFVSKKGVSMTNKAEF 747

BLAST of Bhi12G000322 vs. ExPASy Swiss-Prot
Match: A9QY38 (Subtilisin-like protease 4 OS=Lotus japonicus OX=34305 GN=SBTM4 PE=2 SV=1)

HSP 1 Score: 913.7 bits (2360), Expect = 1.4e-264
Identity = 472/753 (62.68%), Postives = 573/753 (76.10%), Query Frame = 0

Query: 5   FFCIFFLLNFHGIVAMKSELSGTSNQ----IKLQTYIVHVKESELRILGDAIDLESWYTS 64
           F  + FLL FH   A  SEL  T+ +       + YI+HV   E ++L ++ DLESWY S
Sbjct: 6   FIALTFLLTFHVHNAQGSELPTTTTESTETSSSKIYIIHVTGPEGKMLTESEDLESWYHS 65

Query: 65  FLPETIKTSNEQSRLLYSFRNVMSGFSARLT-EEVKAMEEKEGFISARPETILNLHTTHT 124
           FLP T+ +S EQ R++YS++NV+ GF+A LT EE+ A+E+K GFISA P+ +L+  TTHT
Sbjct: 66  FLPPTLMSSEEQPRVIYSYKNVLRGFAASLTQEELSAVEKKNGFISAHPQRVLHRQTTHT 125

Query: 125 PEYLGLNQQFGLWKDSNFGKGVIIGVLDTGISPDHPSFNDDGMPQPPAKWKGRCEFGASI 184
           P++LGL Q  G+WK+SNFGKGVIIGVLD+GI+P HPSF+D G+P PP KWKGRC+   + 
Sbjct: 126 PKFLGLQQDTGVWKESNFGKGVIIGVLDSGITPGHPSFSDVGIPPPPPKWKGRCDLNVTA 185

Query: 185 CNNKLIGARTFNRANSALMG---ESPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVG 244
           CNNKLIGAR FN A  A+ G   E+PIDE+GHGTHTASTAAG FV  AE LGNA+G A G
Sbjct: 186 CNNKLIGARAFNLAAEAMNGKKAEAPIDEDGHGTHTASTAAGAFVNYAEVLGNAKGTAAG 245

Query: 245 MAPLAHLAMYKVCSPKGCSSSDILAALDAAIHDGVDVLSLSLG-APSRPFYIDNIAIGAF 304
           MAP AHLA+YKVC  + C  SDILAALDAA+ DGVDV+S+SLG +   PF+ D+ AIGAF
Sbjct: 246 MAPHAHLAIYKVCFGEDCPESDILAALDAAVEDGVDVISISLGLSEPPPFFNDSTAIGAF 305

Query: 305 AAIKMGIFVSCSAGNSGPSKSTLANEAPWILTVGASTIDRTIMALARLGNGEVHVGESLF 364
           AA++ GIFVSC+AGNSGP  S++ N APWILTVGASTIDR I+A A+LGNG+   GES+F
Sbjct: 306 AAMQKGIFVSCAAGNSGPFNSSIVNAAPWILTVGASTIDRRIVATAKLGNGQEFDGESVF 365

Query: 365 QPRDFPSSFLPLVYAGSSGVKGSEFCVQGSLTNLNVKGKMVVCERGGGIGRIAKGMVVKN 424
           QP  F  + LPL YAG +G + S FC  GSL +   +GK+V+CERGGGI RIAKG  VK 
Sbjct: 366 QPSSFTPTLLPLAYAGKNGKEESAFCANGSLDDSAFRGKVVLCERGGGIARIAKGEEVKR 425

Query: 425 AGGAAMIIINQKADEFSTLAEAHILPATHVSYEAGLKIKSYIRSSQNPMASISFEGTLIG 484
           AGGAAMI++N + + FS  A+ H LPATHVSY AG++IK+YI S+  P A+I F+GT+IG
Sbjct: 426 AGGAAMILMNDETNAFSLSADVHALPATHVSYAAGIEIKAYINSTATPTATILFKGTVIG 485

Query: 485 SRATTFSPAMASFSSRGPCLPSPGILKPDITGPGVNILAAWPFSLDNNTNKKSTFNVISG 544
           +   + +PA+ASFSSRGP LPSPGILKPDI GPGVNILAAWPF L N+T+ K TFN+ SG
Sbjct: 486 N---SLAPAVASFSSRGPNLPSPGILKPDIIGPGVNILAAWPFPLSNSTDSKLTFNIESG 545

Query: 545 TSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTSADVRNRQGRPIVDQDLKPANFFAMGS 604
           TSMSCPHLSGIAAL+KS HP+WSPAAIKSAIMTSAD  N   + IVD+ L+P + FA GS
Sbjct: 546 TSMSCPHLSGIAALLKSSHPHWSPAAIKSAIMTSADTINLGNKLIVDETLQPTDLFATGS 605

Query: 605 GHVNPSKAADPGLVYDIQPDDYIPYLCGL-YSDAQVSIIVRRKVTCSTISRIREGDLNYP 664
           GHVNPS+A DPGLVYDIQPDDYIPYLCGL YS+ +V II  RK+ CS  + I EG+LNYP
Sbjct: 606 GHVNPSRANDPGLVYDIQPDDYIPYLCGLGYSETEVGIIAHRKIKCS--ASIPEGELNYP 665

Query: 665 SFAVTLGPSQMFNRTVTNVGDANSIYSAIVEAPIGVSVKVTPNNLKFSRVIEKVTYSVTF 724
           SF+V LG S+ F RTVTNVG+A+S Y  IV AP GV VKV P  L FS V +K TYSVTF
Sbjct: 666 SFSVELGSSKTFTRTVTNVGEAHSSYDLIVAAPQGVDVKVQPYKLNFSEVNQKETYSVTF 725

Query: 725 SRDDLVRTTSEFSEGYLIWVSNKHMVRSPISVK 748
           SR  L   T E+++G+L WVS KH VRSPISVK
Sbjct: 726 SRTGLGNKTQEYAQGFLKWVSTKHTVRSPISVK 753

BLAST of Bhi12G000322 vs. ExPASy Swiss-Prot
Match: A0A0M3R8G2 (Subtilisin-like protease OS=Petunia hybrida OX=4102 GN=SBT PE=2 SV=1)

HSP 1 Score: 857.4 bits (2214), Expect = 1.2e-247
Identity = 447/748 (59.76%), Postives = 553/748 (73.93%), Query Frame = 0

Query: 6   FCIFFLLNFHGIVAMKSELSGTSNQIKLQTYIVHVKESELRILGDAIDLESWYTSFLPET 65
           F I FLL+FH   A +S          LQTYIVHV + + ++L ++ DLES+Y SFLP T
Sbjct: 7   FSIAFLLSFHLTTAQRS--------TGLQTYIVHVDKPDAQVLANSADLESYYNSFLPAT 66

Query: 66  IKTSNEQSRLLYSFRNVMSGFSARLT-EEVKAMEEKEGFISARPETILNLHTTHTPEYLG 125
           +  S   SR+++S+ +V +GF+A+L+ EEVK ME+K GF+SA+ E +L LHTTHTP +LG
Sbjct: 67  VSGSEVPSRIIHSYHHVATGFAAKLSGEEVKEMEKKAGFVSAKLEKVLTLHTTHTPNFLG 126

Query: 126 LNQQFGLWKDSNFGKGVIIGVLDTGISPDHPSFNDDGMPQPPAKWKGRCEF-GASICNNK 185
           L Q  G W++SN+GKGVIIG+LDTGI+P HPSF+D  MP PPAKWKG+CEF G + CN K
Sbjct: 127 LYQNMGFWQESNYGKGVIIGLLDTGITPGHPSFSDVNMPSPPAKWKGKCEFTGNATCNKK 186

Query: 186 LIGARTFNRANSALMGESPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGMAPLAHL 245
           +IGAR F   +    G  P DE GHGTHTASTAAG FV  A   GNA G AVGMAPLAH+
Sbjct: 187 IIGARNFISGS----GVPPTDEEGHGTHTASTAAGNFVNDANVFGNANGTAVGMAPLAHI 246

Query: 246 AMYKVCSPKGCSSSDILAALDAAIHDGVDVLSLSLGAPSRPFYIDNIAIGAFAAIKMGIF 305
           AMYKVCS  GCS +DILAALDAAI DGVDVLSLSLG  S PFY DNIAIGAFAAI+ GIF
Sbjct: 247 AMYKVCSEDGCSDADILAALDAAIDDGVDVLSLSLGGYSDPFYYDNIAIGAFAAIRKGIF 306

Query: 306 VSCSAGNSGPSKSTLANEAPWILTVGASTIDRTIMALARLGNGEVHVGESLFQPRDFPSS 365
           VS SAGN GP  STL+NEAPWILTVGAST DR I+A A LGNG+ + GES FQP DFP +
Sbjct: 307 VSASAGNDGPLNSTLSNEAPWILTVGASTHDRKIVATAVLGNGQQYDGESAFQPADFPHT 366

Query: 366 FLPLVYAGSSGVKGSEFCVQGSLTNLNVKGKMVVCERGGGIGRIAKGMVVKNAGGAAMII 425
            LPLVY G+S  + + FC  GSL   +VKGK+VVC+RGG + R+ K   VK+AGGAAMI+
Sbjct: 367 LLPLVYPGTSDEEAA-FCSSGSLDKFDVKGKVVVCDRGGDVARLEKSQTVKDAGGAAMIL 426

Query: 426 INQKADEFSTLAEAHILPATHVSYEAGLKIKSYIRSSQNPMASISFEGTLIGSRATTFSP 485
            N + D   T A+AH+LPATHV Y AG  IKSYI S+  P A I F+GT+IG ++   SP
Sbjct: 427 ANLEIDGEGTFADAHVLPATHVGYAAGEMIKSYINSTSTPTAGILFKGTIIGFKS---SP 486

Query: 486 AMASFSSRGPCLPSPGILKPDITGPGVNILAAWPFSLDNNTNKKSTFNVISGTSMSCPHL 545
           +++SFSSRGP L SPGI+KPDI GPGVNILAAWP S++N T    TFN+ISGTSMSCPHL
Sbjct: 487 SVSSFSSRGPNLASPGIVKPDIIGPGVNILAAWPVSVENKTGTDLTFNIISGTSMSCPHL 546

Query: 546 SGIAALIKSVHPNWSPAAIKSAIMTSADVRNRQGRPIVDQDLKPANFFAMGSGHVNPSKA 605
           SGI AL+KS HP+WSPAAIKSAIMTSAD  N +G+PI+D+   PA+ FA G+GHVNPSKA
Sbjct: 547 SGIVALLKSAHPDWSPAAIKSAIMTSADQSNLEGQPILDERNLPADIFATGAGHVNPSKA 606

Query: 606 ADPGLVYDIQPDDYIPYLCGL-YSDAQVSIIVRRKVTCSTISRIREGDLNYPSFAVTLGP 665
           +DPGL+YDIQ +DYI YLCGL Y +  + +IV+  V C   S I E +LNYPSF++ LGP
Sbjct: 607 SDPGLIYDIQLEDYIQYLCGLGYREKDIGLIVQETVKCE--SSISEAELNYPSFSIILGP 666

Query: 666 -SQMFNRTVTNVGDANSIYSAIVEAPIGVSVKVTPNNLKFSRVIEKVTYSVTFSRDDLVR 725
            +Q + RTVTNVGDA+S Y+  +    GV + V P  L F+++ ++ TY+V+F++     
Sbjct: 667 KTQNYTRTVTNVGDASSTYTVNIAKTQGVDIVVEPATLVFTKMYQQATYTVSFTQSG--D 726

Query: 726 TTSEFSEGYLIWVSNKHMVRSPISVKLK 750
            T  F +G + W SN+++VRSPISVKL+
Sbjct: 727 FTDRFVQGAISWSSNEYVVRSPISVKLE 734

BLAST of Bhi12G000322 vs. ExPASy Swiss-Prot
Match: A9QY39 (Subtilisin-like protease 3 OS=Lotus japonicus OX=34305 GN=SBTM3 PE=2 SV=1)

HSP 1 Score: 839.3 bits (2167), Expect = 3.3e-242
Identity = 432/722 (59.83%), Postives = 547/722 (75.76%), Query Frame = 0

Query: 29  NQIKLQTYIVHVKESELRILGDAIDLESWYTSFLPETIKTSNEQSRLLYSFRNVMSGFSA 88
           +Q  L TYIVHV++ ++ I  D  DL ++Y S LPE+ KT+N+  R+++++RNV++GF+ 
Sbjct: 38  DQSNLSTYIVHVRKPQV-IQSD--DLHTFYYSLLPESTKTTNQ--RIVFTYRNVVNGFAV 97

Query: 89  RLT-EEVKAMEEKEGFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNFGKGVIIGVLD 148
           +LT EE KA+++ E  +SARPE IL+LHTTHTP +LGL Q  GLWK SN GKGVIIG+LD
Sbjct: 98  KLTPEEAKALQQNEEVVSARPEKILSLHTTHTPSFLGLQQGLGLWKGSNSGKGVIIGILD 157

Query: 149 TGISPDHPSFNDDGMPQPPAKWKGRCEF-GASICNNKLIGARTFNRANSALMGESPIDEN 208
           TGISP HPSF+D+GMP PPAKW G CEF G   CNNK+IGAR F +  +  +   P D+ 
Sbjct: 158 TGISPFHPSFSDEGMPSPPAKWNGICEFTGKRTCNNKIIGARNFVKTKNLTL---PFDDV 217

Query: 209 GHGTHTASTAAGTFVEGAEALGNARGKAVGMAPLAHLAMYKVCSPKGCSSSDILAALDAA 268
           GHGTHTASTAAG  V+GA   GNA G AVGMAP AH+AMYKVC   GCS S ILA +D A
Sbjct: 218 GHGTHTASTAAGRPVQGANVYGNANGTAVGMAPDAHIAMYKVCGLVGCSESAILAGMDTA 277

Query: 269 IHDGVDVLSLSLGAPSRPFYIDNIAIGAFAAIKMGIFVSCSAGNSGPSKSTLANEAPWIL 328
           + DGVDVLSLSLG PS PF+ D IA+GAF AI+ GIFVSCSA NSGP+ S+L+NEAPWIL
Sbjct: 278 VDDGVDVLSLSLGGPSGPFFEDPIALGAFGAIQKGIFVSCSAANSGPAYSSLSNEAPWIL 337

Query: 329 TVGASTIDRTIMALARLGNGEVHVGESLFQPRDFPSSFLPLVYAGSSGVKG-SEFCVQGS 388
           TVGAS+IDRTIMA A+LGNG+ +VG+S+FQP+DF  S LPLVYAG++G    S FC   S
Sbjct: 338 TVGASSIDRTIMATAKLGNGKEYVGQSVFQPKDFAPSLLPLVYAGANGNNNFSVFCAPES 397

Query: 389 LTNLNVKGKMVVCERGGGIGRIAKGMVVKNAGGAAMIIINQKADEFSTLAEAHILPATHV 448
           L   +V+GK+V+CE GG + R+ KG  VK+AGGAAMI++N   ++F+ +A+ H+LPA H+
Sbjct: 398 LNRSDVEGKVVLCEDGGFVPRVFKGKAVKDAGGAAMILMNSVLEDFNPIADVHVLPAVHI 457

Query: 449 SYEAGLKIKSYIRSSQNPMASISFEGTLIGSRATTFSPAMASFSSRGPCLPSPGILKPDI 508
           SYEAGL +K YI S+  P A+I FEGT+IG+     +P + SFSSRGP   SPGILKPDI
Sbjct: 458 SYEAGLALKEYINSTSTPTATILFEGTVIGN---LLAPQVTSFSSRGPSKASPGILKPDI 517

Query: 509 TGPGVNILAAWPFSLDNNTNKKSTFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSA 568
            GPG+NILAAWP SLDN+T     FN+ISGTSMSCPHLSGIAAL+K+ HP+WSPAAIKSA
Sbjct: 518 IGPGLNILAAWPVSLDNSTT--PPFNIISGTSMSCPHLSGIAALLKNSHPDWSPAAIKSA 577

Query: 569 IMTSADVRNRQGRPIVDQDLKPANFFAMGSGHVNPSKAADPGLVYDIQPDDYIPYLCGL- 628
           IMT+A   N  G PI+DQ L PA+ FA G+GHVNP KA DPGLVYDI+P+DYIPYLCGL 
Sbjct: 578 IMTTASQVNLGGTPILDQRLVPADVFATGAGHVNPVKANDPGLVYDIEPNDYIPYLCGLN 637

Query: 629 YSDAQVSIIVRRKVTCSTISRIREGDLNYPSFAVTLG-PSQMFNRTVTNVGDANSIYSAI 688
           Y+D +V +I++++V CS ++ I E +LNYPSF++ LG  +Q++ RTV NVG ANS Y+A 
Sbjct: 638 YTDREVGVILQQRVRCSEVNHIAEAELNYPSFSILLGNTTQLYTRTVANVGPANSTYTAE 697

Query: 689 VEAPIGVSVKVTPNNLKFSRVIEKVTYSVTFSRDDLVRTTSEFSEGYLIWVSNKHMVRSP 746
           +  P+GV + ++P  L F+ V +K+TYSV+F      R    F++G L WVS K+ VRSP
Sbjct: 698 IGVPVGVGMSLSPAQLTFTEVGQKLTYSVSFIPFSEDRDNHTFAQGSLKWVSGKYSVRSP 746

BLAST of Bhi12G000322 vs. ExPASy Swiss-Prot
Match: A9QY40 (Subtilisin-like protease 1 OS=Lotus japonicus OX=34305 GN=SBTM1 PE=2 SV=1)

HSP 1 Score: 822.8 bits (2124), Expect = 3.2e-237
Identity = 437/726 (60.19%), Postives = 536/726 (73.83%), Query Frame = 0

Query: 33  LQTYIVHVKESELR-ILGDAIDLESWYTSFLPETIKTSNEQSRLLYSFRNVMSGFSARLT 92
           L TYIVHVK+ E+   L    +L +W+ SFLPET    + + R+++S+RNV SGF+ RLT
Sbjct: 38  LTTYIVHVKKLEIEGPLQSTEELHTWHHSFLPET----SNKDRMVFSYRNVASGFAVRLT 97

Query: 93  -EEVKAMEEKEGFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNFGKGVIIGVLDTGI 152
            EE  A++EKE  +S RPE  L+LHTTHTP +LGL Q  GLW DSN GKGVIIGV+DTGI
Sbjct: 98  PEEANALQEKEEVMSIRPERTLSLHTTHTPSFLGLRQGQGLWNDSNLGKGVIIGVIDTGI 157

Query: 153 SPDHPSFNDDGMPQPPAKWKGRCEF-GASICNNKLIGARTFNRANSALMGESPIDENGHG 212
            P H SFND+GMP PPAKWKG CEF G S+CNNKLIGAR  N   SA+  E P ++  HG
Sbjct: 158 YPFHLSFNDEGMPPPPAKWKGHCEFTGGSVCNNKLIGAR--NLVKSAIQ-EPPYEDFFHG 217

Query: 213 THTASTAAGTFVEGAEALGNARGKAVGMAPLAHLAMYKVCSPK---GCSSSDILAALDAA 272
           THTA+ AAG FVEGA   GNARG A GMAP AHLA+YKVCS K    C  S ILAA+D A
Sbjct: 218 THTAAEAAGRFVEGASVFGNARGTAAGMAPDAHLAIYKVCSSKVKDECPESAILAAMDIA 277

Query: 273 IHDGVDVLSLSLGAPSRPFYIDNIAIGAFAAIKMGIFVSCSAGNSGPSKSTLANEAPWIL 332
           I DGVDVLSLSLG  S PF+ D IAIGAFAA + GIFVSCSA NSGP  S+L+NEAPWIL
Sbjct: 278 IEDGVDVLSLSLGLGSLPFFEDPIAIGAFAATQKGIFVSCSAANSGPHYSSLSNEAPWIL 337

Query: 333 TVGASTIDRTIMALARLGNGEVHVGESLFQPRDFPSSFLPLVYAGSSGVKGSEFCVQGSL 392
           TVGASTIDR I A A+LGNG  + GE+LFQP+DF S  LPLVYA +     S  C  GSL
Sbjct: 338 TVGASTIDRKISASAKLGNGAEYEGETLFQPKDFSSQLLPLVYAAAEKNNSSALCAPGSL 397

Query: 393 TNLNVKGKMVVCERGGGIGRIAKGMVVKNAGGAAMIIINQKADEFSTLAEAHILPATHVS 452
            N+NVKGK+VVC+ GGGI  IAKG  V +AGG+AMI+ N +   F+TLA AH+LPA HVS
Sbjct: 398 RNINVKGKVVVCDLGGGIPFIAKGQEVLDAGGSAMILANIENFGFTTLANAHVLPAVHVS 457

Query: 453 YEAGLKIKSYIRSSQNPMASISFEGTLIGSRATTFSPAMASFSSRGPCLPSPGILKPDIT 512
           Y A L IK+YI S+  P A++ F+GT+IG    + +P++A+FSSRGP   SPGILKPDI 
Sbjct: 458 YAASLAIKAYINSTYTPTATVLFQGTIIGD---SLAPSVAAFSSRGPSQQSPGILKPDII 517

Query: 513 GPGVNILAAWPFSLDNNTNKKSTFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAI 572
           GPGVNILAAW  S+D   NK   F++ISGTSMSCPHLSGIAAL+KS HP+WSPAAIKSAI
Sbjct: 518 GPGVNILAAWAVSVD---NKIPAFDIISGTSMSCPHLSGIAALLKSAHPDWSPAAIKSAI 577

Query: 573 MTSADVRNRQGRPIVDQDLKPANFFAMGSGHVNPSKAADPGLVYDIQPDDYIPYLCGL-Y 632
           MT+A+  N +G PI+DQ L+PA+ FA G+GHVNP +A DPGLVYDIQP+DY+PYLCGL Y
Sbjct: 578 MTTANTLNLRGLPILDQRLQPADIFATGAGHVNPVRANDPGLVYDIQPEDYVPYLCGLGY 637

Query: 633 SDAQVSIIVRRKVTCSTISRIREGDLNYPSFAVTLG-PSQMFNRTVTNVGDANSIYSAIV 692
           SD +V+IIV+R V C  +  I + +LNYPSF++ LG  SQ + RT+TNVG ANS Y+  +
Sbjct: 638 SDREVTIIVQRSVRCFNVKSIAQAELNYPSFSILLGSDSQFYTRTLTNVGPANSTYTVKI 697

Query: 693 EAPIGVSVKVTPNNLKFSRVIEKVTYSVTF-SRDDLVRTTSEFSEGYLIWVSNKHMVRSP 750
           + P+ + + V+P+ + F++V +KV Y V F  +    R    F++G + WVS+KH+VR+P
Sbjct: 698 DVPLAMGISVSPSQITFTQVNQKVAYFVDFIPQIKENRGNHTFAQGAITWVSDKHVVRTP 750

BLAST of Bhi12G000322 vs. ExPASy Swiss-Prot
Match: G7KEU7 (Subtilisin-like protease OS=Medicago truncatula OX=3880 GN=SBT PE=2 SV=1)

HSP 1 Score: 808.9 bits (2088), Expect = 4.8e-233
Identity = 435/752 (57.85%), Postives = 536/752 (71.28%), Query Frame = 0

Query: 8   IFFLLNFHGIVAMKSELSGTSNQIKLQTYIVHVKESELRILGDAIDLESWYTSFLPETIK 67
           + F+L    ++A +  L    +QI L TYIVHVK+SE      + DL SWY SFLP+T  
Sbjct: 12  LIFILCSISMLAAEENLE--HDQINLMTYIVHVKKSENVASHQSEDLHSWYHSFLPQTFP 71

Query: 68  TSNEQSRLLYSFRNVMSGFSARLT-EEVKAMEEKEGFISARPETILNLHTTHTPEYLGLN 127
               + R+++S+R V SGF+ +LT EE K+++EK   +SARPE  L LHTTHTP +LGL 
Sbjct: 72  ---HKERMVFSYRKVASGFAVKLTPEEAKSLQEKGEIVSARPERTLELHTTHTPTFLGLK 131

Query: 128 QQFGLWKDSNFGKGVIIGVLDTGISPDHPSFNDDGMPQPPAKWKGRCEF-GASICNNKLI 187
           Q  GLW D N GKGVIIG++DTGI P HPSFND+GMP PPAKWKG CEF G  +CNNKLI
Sbjct: 132 QGQGLWSDDNLGKGVIIGIIDTGIFPLHPSFNDEGMPPPPAKWKGHCEFTGGQVCNNKLI 191

Query: 188 GARTFNRANSALMGESPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGMAPLAHLAM 247
           GAR  N   SA+  E P +   HGTHTA+ AAG F+E A   GNA+G A GMAP AHLA+
Sbjct: 192 GAR--NLVKSAIQ-EPPFENFFHGTHTAAEAAGRFIEDASVFGNAKGVAAGMAPNAHLAI 251

Query: 248 YKVCSPK-GCSSSDILAALDAAIHDGVDVLSLSLGAPSRPFYIDNIAIGAFAAIKMGIFV 307
           YKVC+ K GC+ S ILAA+D AI DGVDVLSLSLG  S PF+ D IAIGAFAA + G+FV
Sbjct: 252 YKVCNDKIGCTESAILAAMDIAIEDGVDVLSLSLGLGSLPFFEDPIAIGAFAATQNGVFV 311

Query: 308 SCSAGNSGPSKSTLANEAPWILTVGASTIDRTIMALARLGNGEVHVGESLFQPRDFPSSF 367
           SCSA NSGP  STL+NEAPWILTVGASTIDR I+A A+LGNGE + GE+LFQP+DF    
Sbjct: 312 SCSAANSGPGYSTLSNEAPWILTVGASTIDRKIVASAKLGNGEEYEGETLFQPKDFSQQL 371

Query: 368 LPLVYAGSSG----VKGSEFCVQGSLTNLNVKGKMVVCERGGGIGRIAKGMVVKNAGGAA 427
           LPLVY GS G     +    C+ GSL N+++ GK+V+C+  G +  I KG  V N+GG A
Sbjct: 372 LPLVYPGSFGYGNQTQNQSLCLPGSLKNIDLSGKVVLCD-VGNVSSIVKGQEVLNSGGIA 431

Query: 428 MIIINQKADEFSTLAEAHILPATHVSYEAGLKIKSYIRSSQNPMASISFEGTLIGSRATT 487
           MI+ N +A  FST A AH+LPA  VSY AGL IKSYI+S+ NP A++ F+GT+IG    +
Sbjct: 432 MILANSEALGFSTFAIAHVLPAVEVSYAAGLTIKSYIKSTYNPTATLIFKGTIIGD---S 491

Query: 488 FSPAMASFSSRGPCLPSPGILKPDITGPGVNILAAWPFSLDNNTNKKSTFNVISGTSMSC 547
            +P++  FSSRGP   SPGILKPDI GPGVNILAAW  S+D   NK   F+++SGTSMSC
Sbjct: 492 LAPSVVYFSSRGPSQESPGILKPDIIGPGVNILAAWAVSVD---NKIPAFDIVSGTSMSC 551

Query: 548 PHLSGIAALIKSVHPNWSPAAIKSAIMTSADVRNRQGRPIVDQDLKPANFFAMGSGHVNP 607
           PHLSGIAALIKS HP+WSPAAIKSAIMT+A+  N  G PI+DQ L PA+ FA G+GHVNP
Sbjct: 552 PHLSGIAALIKSSHPDWSPAAIKSAIMTTANTLNLGGIPILDQRLFPADIFATGAGHVNP 611

Query: 608 SKAADPGLVYDIQPDDYIPYLCGL-YSDAQVSIIVRRKVTCSTISRIREGDLNYPSFAVT 667
            KA DPGLVYDI+P+DY+PYLCGL YSD ++ +IV+ KV CS +  I E  LNYPSF++ 
Sbjct: 612 VKANDPGLVYDIEPEDYVPYLCGLGYSDKEIEVIVQWKVKCSNVKSIPEAQLNYPSFSIL 671

Query: 668 LG-PSQMFNRTVTNVGDANSIYSAIVEAPIGVSVKVTPNNLKFSRVIEKVTYSVTF-SRD 727
           LG  SQ + RT+TNVG ANS Y   +E P+ + + V P+ + F+ V EKV++SV F  + 
Sbjct: 672 LGSDSQYYTRTLTNVGFANSTYKVELEVPLALGMSVNPSEITFTEVNEKVSFSVEFIPQI 731

Query: 728 DLVRTTSEFSEGYLIWVSNKHMVRSPISVKLK 750
              R    F +G L WVS++H VR PISV  K
Sbjct: 732 KENRRNHTFGQGSLTWVSDRHAVRIPISVIFK 748

BLAST of Bhi12G000322 vs. NCBI nr
Match: XP_038906452.1 (subtilisin-like protease 4 [Benincasa hispida])

HSP 1 Score: 1446.8 bits (3744), Expect = 0.0e+00
Identity = 730/730 (100.00%), Postives = 730/730 (100.00%), Query Frame = 0

Query: 20  MKSELSGTSNQIKLQTYIVHVKESELRILGDAIDLESWYTSFLPETIKTSNEQSRLLYSF 79
           MKSELSGTSNQIKLQTYIVHVKESELRILGDAIDLESWYTSFLPETIKTSNEQSRLLYSF
Sbjct: 1   MKSELSGTSNQIKLQTYIVHVKESELRILGDAIDLESWYTSFLPETIKTSNEQSRLLYSF 60

Query: 80  RNVMSGFSARLTEEVKAMEEKEGFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNFGK 139
           RNVMSGFSARLTEEVKAMEEKEGFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNFGK
Sbjct: 61  RNVMSGFSARLTEEVKAMEEKEGFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNFGK 120

Query: 140 GVIIGVLDTGISPDHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFNRANSALMG 199
           GVIIGVLDTGISPDHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFNRANSALMG
Sbjct: 121 GVIIGVLDTGISPDHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFNRANSALMG 180

Query: 200 ESPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGMAPLAHLAMYKVCSPKGCSSSDI 259
           ESPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGMAPLAHLAMYKVCSPKGCSSSDI
Sbjct: 181 ESPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGMAPLAHLAMYKVCSPKGCSSSDI 240

Query: 260 LAALDAAIHDGVDVLSLSLGAPSRPFYIDNIAIGAFAAIKMGIFVSCSAGNSGPSKSTLA 319
           LAALDAAIHDGVDVLSLSLGAPSRPFYIDNIAIGAFAAIKMGIFVSCSAGNSGPSKSTLA
Sbjct: 241 LAALDAAIHDGVDVLSLSLGAPSRPFYIDNIAIGAFAAIKMGIFVSCSAGNSGPSKSTLA 300

Query: 320 NEAPWILTVGASTIDRTIMALARLGNGEVHVGESLFQPRDFPSSFLPLVYAGSSGVKGSE 379
           NEAPWILTVGASTIDRTIMALARLGNGEVHVGESLFQPRDFPSSFLPLVYAGSSGVKGSE
Sbjct: 301 NEAPWILTVGASTIDRTIMALARLGNGEVHVGESLFQPRDFPSSFLPLVYAGSSGVKGSE 360

Query: 380 FCVQGSLTNLNVKGKMVVCERGGGIGRIAKGMVVKNAGGAAMIIINQKADEFSTLAEAHI 439
           FCVQGSLTNLNVKGKMVVCERGGGIGRIAKGMVVKNAGGAAMIIINQKADEFSTLAEAHI
Sbjct: 361 FCVQGSLTNLNVKGKMVVCERGGGIGRIAKGMVVKNAGGAAMIIINQKADEFSTLAEAHI 420

Query: 440 LPATHVSYEAGLKIKSYIRSSQNPMASISFEGTLIGSRATTFSPAMASFSSRGPCLPSPG 499
           LPATHVSYEAGLKIKSYIRSSQNPMASISFEGTLIGSRATTFSPAMASFSSRGPCLPSPG
Sbjct: 421 LPATHVSYEAGLKIKSYIRSSQNPMASISFEGTLIGSRATTFSPAMASFSSRGPCLPSPG 480

Query: 500 ILKPDITGPGVNILAAWPFSLDNNTNKKSTFNVISGTSMSCPHLSGIAALIKSVHPNWSP 559
           ILKPDITGPGVNILAAWPFSLDNNTNKKSTFNVISGTSMSCPHLSGIAALIKSVHPNWSP
Sbjct: 481 ILKPDITGPGVNILAAWPFSLDNNTNKKSTFNVISGTSMSCPHLSGIAALIKSVHPNWSP 540

Query: 560 AAIKSAIMTSADVRNRQGRPIVDQDLKPANFFAMGSGHVNPSKAADPGLVYDIQPDDYIP 619
           AAIKSAIMTSADVRNRQGRPIVDQDLKPANFFAMGSGHVNPSKAADPGLVYDIQPDDYIP
Sbjct: 541 AAIKSAIMTSADVRNRQGRPIVDQDLKPANFFAMGSGHVNPSKAADPGLVYDIQPDDYIP 600

Query: 620 YLCGLYSDAQVSIIVRRKVTCSTISRIREGDLNYPSFAVTLGPSQMFNRTVTNVGDANSI 679
           YLCGLYSDAQVSIIVRRKVTCSTISRIREGDLNYPSFAVTLGPSQMFNRTVTNVGDANSI
Sbjct: 601 YLCGLYSDAQVSIIVRRKVTCSTISRIREGDLNYPSFAVTLGPSQMFNRTVTNVGDANSI 660

Query: 680 YSAIVEAPIGVSVKVTPNNLKFSRVIEKVTYSVTFSRDDLVRTTSEFSEGYLIWVSNKHM 739
           YSAIVEAPIGVSVKVTPNNLKFSRVIEKVTYSVTFSRDDLVRTTSEFSEGYLIWVSNKHM
Sbjct: 661 YSAIVEAPIGVSVKVTPNNLKFSRVIEKVTYSVTFSRDDLVRTTSEFSEGYLIWVSNKHM 720

Query: 740 VRSPISVKLK 750
           VRSPISVKLK
Sbjct: 721 VRSPISVKLK 730

BLAST of Bhi12G000322 vs. NCBI nr
Match: XP_004147599.2 (subtilisin-like protease SBT1.7 [Cucumis sativus] >KAE8647878.1 hypothetical protein Csa_000520 [Cucumis sativus])

HSP 1 Score: 1247.6 bits (3227), Expect = 0.0e+00
Identity = 629/751 (83.75%), Postives = 684/751 (91.08%), Query Frame = 0

Query: 3   LPFFCIFFLLNFHGIVAMKSELSGTSNQIKLQTYIVHVKESELRILGDAIDLESWYTSFL 62
           LPF CIFF LNFHG VAMK+EL   SN   LQTYIVHVK+ E+ ILGD IDL++WYTSFL
Sbjct: 4   LPFICIFFFLNFHGYVAMKTELWSASN-TNLQTYIVHVKQPEVEILGDTIDLQNWYTSFL 63

Query: 63  PETIK-TSNEQSRLLYSFRNVMSGFSARLT-EEVKAMEEKEGFISARPETILNLHTTHTP 122
           PETI+ +SNEQSRLLYS+R+V+SGFSARLT E+VK MEEK+GFISA PET LNLHTTHTP
Sbjct: 64  PETIEASSNEQSRLLYSYRHVISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTP 123

Query: 123 EYLGLNQQFGLWKDSNFGKGVIIGVLDTGISPDHPSFNDDGMPQPPAKWKGRCEFGASIC 182
           EYLGLNQ FGLWK+SNFGKGVIIGVLDTGI P+HPSFND+GMP PPAKWKGRCEFGASIC
Sbjct: 124 EYLGLNQHFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFGASIC 183

Query: 183 NNKLIGARTFNRANSALMGESPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGMAPL 242
           NNKLIGARTFN AN+  +G+SP DENGHGTHTASTAAGTFV+GAEALGNARGKAVGMAPL
Sbjct: 184 NNKLIGARTFNLANNVSIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPL 243

Query: 243 AHLAMYKVCSPKGCSSSDILAALDAAIHDGVDVLSLSLGAPSRPFYIDNIAIGAFAAIKM 302
           AH+A+YKVCSPKGCSSSDILAALDAAI DGVDVLSLSLGAPS PF+ D IA+GAFAAIK 
Sbjct: 244 AHIAVYKVCSPKGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKK 303

Query: 303 GIFVSCSAGNSGPSKSTLANEAPWILTVGASTIDRTIMALARLGNGEVHVGESLFQPRDF 362
           GIFVSCSAGNSGPSK+TLANEAPWILTVGASTIDR I+ALA+L +G+V  GESLFQPRDF
Sbjct: 304 GIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDF 363

Query: 363 PSSFLPLVYAGSSGVKGSEFCVQGSLTNLNVKGKMVVCERGGGIGRIAKGMVVKNAGGAA 422
            S FLPLVYAG SG++GSE+CV+GSL  LNV GK+VVCERGGGIGRIAKG+VVKN GGAA
Sbjct: 364 SSKFLPLVYAGKSGIEGSEYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAA 423

Query: 423 MIIINQKADEFSTLAEAHILPATHVSYEAGLKIKSYIRSSQNPMASISFEGTLIGSRATT 482
           MI++NQK D FSTLAEAH+LP TH+SYE GLKIK YI SS NP ASISFEGTL+G+RATT
Sbjct: 424 MILVNQKPDGFSTLAEAHVLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATT 483

Query: 483 FSPAMASFSSRGPCLPSPGILKPDITGPGVNILAAWPFSLDN--NTNKKSTFNVISGTSM 542
           FSPAMASFSSRGPC  SPGILKPDITGPGVNILAAWPF L+N  NTN KSTFNVISGTSM
Sbjct: 484 FSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSM 543

Query: 543 SCPHLSGIAALIKSVHPNWSPAAIKSAIMTSADVRNRQGRPIVDQDLKPANFFAMGSGHV 602
           SCPHLSGIAALIKS HPNWSPAAIKSAIMTSADVRN QG+PIVDQDLKPANFFAMGSGHV
Sbjct: 544 SCPHLSGIAALIKSNHPNWSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHV 603

Query: 603 NPSKAADPGLVYDIQPDDYIPYLCGLYSDAQVSIIVRRKVTCSTISRIREGDLNYPSFAV 662
           NPSKAA+PGLVYDIQPDDY+PYLC LY+DAQVSIIVRR+VTCST+SRIREGDLNYPSFAV
Sbjct: 604 NPSKAANPGLVYDIQPDDYVPYLCHLYTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAV 663

Query: 663 TLG-PSQMFNRTVTNVGDANSIYSAIVEAPIGVSVKVTPNNLKFSRVIEKVTYSVTFSRD 722
           +LG  SQ FNRTVTNVGDANS+Y AIV+AP GVSV+VTP NLKFS++ EK+TYSVTFSR 
Sbjct: 664 SLGADSQAFNRTVTNVGDANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRI 723

Query: 723 DLVRTTSEFSEGYLIWVSNKHMVRSPISVKL 749
           D VRT SEFSEGYLIWVSNKH+VRSPISVKL
Sbjct: 724 DFVRTRSEFSEGYLIWVSNKHIVRSPISVKL 753

BLAST of Bhi12G000322 vs. NCBI nr
Match: XP_008437177.1 (PREDICTED: subtilisin-like protease SBT1.7 [Cucumis melo])

HSP 1 Score: 1243.8 bits (3217), Expect = 0.0e+00
Identity = 628/751 (83.62%), Postives = 685/751 (91.21%), Query Frame = 0

Query: 3   LPFFCIFFLLNFHGIVAMKSELSGTSNQIKLQTYIVHVKESELRILGDAIDLESWYTSFL 62
           LPF CIFF LNFHG VAMK+ELS TSN   LQTYIVHVK+  L ILGD IDL++WYTSFL
Sbjct: 4   LPFICIFFFLNFHGYVAMKTELSSTSN-TNLQTYIVHVKQPVLEILGDTIDLQNWYTSFL 63

Query: 63  PETIK-TSNEQSRLLYSFRNVMSGFSARLT-EEVKAMEEKEGFISARPETILNLHTTHTP 122
           PETI+ +S+EQ RLLYS+R+VMSGFSARLT E+VKAMEEK+GFISA PET LNLHTTHTP
Sbjct: 64  PETIEASSDEQPRLLYSYRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTP 123

Query: 123 EYLGLNQQFGLWKDSNFGKGVIIGVLDTGISPDHPSFNDDGMPQPPAKWKGRCEFGASIC 182
           EYLGLN+ FGLWK+SNFGKGVIIGVLDTGI P+HPSFND+GM  PPAKWKGRCEFGASIC
Sbjct: 124 EYLGLNKHFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASIC 183

Query: 183 NNKLIGARTFNRANSALMGESPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGMAPL 242
           NNKLIGARTFN AN+ ++G+SP DENGHGTHTASTAAGTFV+GAEALGNARGKAVGMAPL
Sbjct: 184 NNKLIGARTFNLANNFVIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPL 243

Query: 243 AHLAMYKVCSPKGCSSSDILAALDAAIHDGVDVLSLSLGAPSRPFYIDNIAIGAFAAIKM 302
           AH+AMYKVCSP GCSSSDILAALDAAI DGVDVLSLSLGAPS PF+ D IA+GAFAAIK 
Sbjct: 244 AHIAMYKVCSPNGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAMGAFAAIKK 303

Query: 303 GIFVSCSAGNSGPSKSTLANEAPWILTVGASTIDRTIMALARLGNGEVHVGESLFQPRDF 362
           GIFVSCSAGNSGPSK+TLANEAPWILTVGASTIDR I+ALA+LG+GEV+ GESLFQPR+F
Sbjct: 304 GIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNF 363

Query: 363 PSSFLPLVYAGSSGVKGSEFCVQGSLTNLNVKGKMVVCERGGGIGRIAKGMVVKNAGGAA 422
           PS FLPLVYAG SG++GSE+C+QGSL  LNV GK+VVCERGGGI R+AKG+VVKN GGAA
Sbjct: 364 PSKFLPLVYAGKSGIEGSEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGGAA 423

Query: 423 MIIINQKADEFSTLAEAHILPATHVSYEAGLKIKSYIRSSQNPMASISFEGTLIGSRATT 482
           MI+INQK + FSTLAEAH+LP TH+SYE GLKIK+YI SS NP ASISF+GTL+G+RATT
Sbjct: 424 MILINQKPEGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRATT 483

Query: 483 FSPAMASFSSRGPCLPSPGILKPDITGPGVNILAAWPFSLDN--NTNKKSTFNVISGTSM 542
           FSPAMASFSSRGPC  SPGILKPDITGPGVNILAAWPF L+N  NTN KSTFNVISGTSM
Sbjct: 484 FSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSM 543

Query: 543 SCPHLSGIAALIKSVHPNWSPAAIKSAIMTSADVRNRQGRPIVDQDLKPANFFAMGSGHV 602
           SCPHLSGIAALIKS HPNWSPAAIKSAIMTSADVRN QG+PI+DQDLKPANFFAMGSGHV
Sbjct: 544 SCPHLSGIAALIKSYHPNWSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSGHV 603

Query: 603 NPSKAADPGLVYDIQPDDYIPYLCGLYSDAQVSIIVRRKVTCSTISRIREGDLNYPSFAV 662
           NPSKAA+PGLVYDIQPDDYIPYLC LY+DAQVSIIVR++VTCST+ RIREGDLNYPSFAV
Sbjct: 604 NPSKAANPGLVYDIQPDDYIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSFAV 663

Query: 663 TLGP-SQMFNRTVTNVGDANSIYSAIVEAPIGVSVKVTPNNLKFSRVIEKVTYSVTFSRD 722
           +LG  SQ FNRTVTNVGDANS+Y  IVEAP GVSVKVTP+NLKFS++ EKVTYSVTFSR 
Sbjct: 664 SLGAGSQTFNRTVTNVGDANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFSRI 723

Query: 723 DLVRTTSEFSEGYLIWVSNKHMVRSPISVKL 749
           D VRTTSEFSEGYLIWVS K MVRSPISVKL
Sbjct: 724 DFVRTTSEFSEGYLIWVSKKLMVRSPISVKL 753

BLAST of Bhi12G000322 vs. NCBI nr
Match: KAA0042822.1 (subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa])

HSP 1 Score: 1213.7 bits (3139), Expect = 0.0e+00
Identity = 614/734 (83.65%), Postives = 669/734 (91.14%), Query Frame = 0

Query: 20  MKSELSGTSNQIKLQTYIVHVKESELRILGDAIDLESWYTSFLPETIK-TSNEQSRLLYS 79
           MK+ELS TSN   LQTYIVHVK+  L ILGD IDL++WYTSFLPETI+ +S+EQ RLLYS
Sbjct: 1   MKTELSSTSN-TNLQTYIVHVKQPVLEILGDTIDLQNWYTSFLPETIEASSDEQPRLLYS 60

Query: 80  FRNVMSGFSARLT-EEVKAMEEKEGFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNF 139
           +R+VMSGFSARLT E+VKAMEEK+GFISA PET LNLHTTHTPEYLGLN  FGLWK+SNF
Sbjct: 61  YRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNTHFGLWKNSNF 120

Query: 140 GKGVIIGVLDTGISPDHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFNRANSAL 199
           GKGVIIGVLDTGI P+HPSFND+GM  PPAKWKGRCEFGASICNNKLIGARTFN AN+ +
Sbjct: 121 GKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNFV 180

Query: 200 MGESPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGMAPLAHLAMYKVCSPKGCSSS 259
           +G+SP DENGHGTHTASTAAGTFV+GAEALGNARGKAVGMAPLAH+AMYKVCSP GCSSS
Sbjct: 181 IGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSS 240

Query: 260 DILAALDAAIHDGVDVLSLSLGAPSRPFYIDNIAIGAFAAIKMGIFVSCSAGNSGPSKST 319
           DILAALDAAI DGVDVLSLSLGAPS PF+ D IAIGAFAAIK GIFVSCSAGNSGPSK+T
Sbjct: 241 DILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAIGAFAAIKKGIFVSCSAGNSGPSKNT 300

Query: 320 LANEAPWILTVGASTIDRTIMALARLGNGEVHVGESLFQPRDFPSSFLPLVYAGSSGVKG 379
           LANEAPWILTVGASTIDR I+ALA+LG+GEV+ GESLFQPR+FPS FLPLVYAG SG++G
Sbjct: 301 LANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIEG 360

Query: 380 SEFCVQGSLTNLNVKGKMVVCERGGGIGRIAKGMVVKNAGGAAMIIINQKADEFSTLAEA 439
           SE+C+QGSL  LNV GK+VVCERGGGI R+AKG+VVKN GGAAMI+INQK + FSTLAEA
Sbjct: 361 SEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAEA 420

Query: 440 HILPATHVSYEAGLKIKSYIRSSQNPMASISFEGTLIGSRATTFSPAMASFSSRGPCLPS 499
           H+LP TH+SYE GLKIK+YI SS NP ASISF+GTL+G+RATTFSPAMASFSSRGPC  S
Sbjct: 421 HVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQAS 480

Query: 500 PGILKPDITGPGVNILAAWPFSLDN--NTNKKSTFNVISGTSMSCPHLSGIAALIKSVHP 559
           PGILKPDITGPGVNILAAWPF L+N  NTN KSTFNVISGTSMSCPHLSGIAALIKS HP
Sbjct: 481 PGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYHP 540

Query: 560 NWSPAAIKSAIMTSADVRNRQGRPIVDQDLKPANFFAMGSGHVNPSKAADPGLVYDIQPD 619
           NWSPAAIKSAIMTSADVRN QG+PI+DQDLKPANFFAMGSGHVNPSKAA+PGLVYDIQPD
Sbjct: 541 NWSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPD 600

Query: 620 DYIPYLCGLYSDAQVSIIVRRKVTCSTISRIREGDLNYPSFAVTL-GPSQMFNRTVTNVG 679
           DYIPYLC LY+DAQVSIIVR++VTCST+ RIREGDLNYPSFAV+L   SQ FNRTVTNVG
Sbjct: 601 DYIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLEAGSQTFNRTVTNVG 660

Query: 680 DANSIYSAIVEAPIGVSVKVTPNNLKFSRVIEKVTYSVTFSRDDLVRTTSEFSEGYLIWV 739
           DANS+Y  IVEAP GVSVKVTP+NLKFS++ EKVTYSVTFSR D VRTTSEFSEGYLIWV
Sbjct: 661 DANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIWV 720

Query: 740 SNKHMVRSPISVKL 749
           S K MVRSPISVKL
Sbjct: 721 SKKLMVRSPISVKL 733

BLAST of Bhi12G000322 vs. NCBI nr
Match: KAA0042821.1 (subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa])

HSP 1 Score: 1087.0 bits (2810), Expect = 0.0e+00
Identity = 544/752 (72.34%), Postives = 636/752 (84.57%), Query Frame = 0

Query: 1   MVLPFFCIFFLLNFHGIVAMKSELSGTSNQIKLQTYIVHVKESELRILGDAID--LESWY 60
           +VL F  IFFLLN    VA K EL  T  Q KLQTYIVHV++ ++R++G++ +  +ESWY
Sbjct: 2   VVLRFLSIFFLLNIESHVAAKVELPTTPLQSKLQTYIVHVEQPKIRLVGESSNDHIESWY 61

Query: 61  TSFLPETIKTSNEQSRLLYSFRNVMSGFSARLT-EEVKAMEEKEGFISARPETILNLHTT 120
            SF+P++ +T+ EQ +LLYS+RNVMSGFSARLT E+VKAME+K+GFISA PETI++LHTT
Sbjct: 62  MSFIPKSTETTVEQPQLLYSYRNVMSGFSARLTIEQVKAMEKKDGFISAMPETIMSLHTT 121

Query: 121 HTPEYLGLNQQFGLWKDSNFGKGVIIGVLDTGISPDHPSFNDDGMPQPPAKWKGRCEFGA 180
           HTPEYLGLNQQFG+WK+SNFGKGVI+GVLDTGI P+HPSFND+GM  PPAKWKG+CEF +
Sbjct: 122 HTPEYLGLNQQFGIWKNSNFGKGVIVGVLDTGIHPNHPSFNDEGMSPPPAKWKGKCEFNS 181

Query: 181 SICNNKLIGARTFNRANSALMGESPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGM 240
           S+CNNKLIGARTFN  N+ LM ESP DE GHGTHTASTAAG FVE AEALGNA+GKA G+
Sbjct: 182 SLCNNKLIGARTFNLGNNFLMEESPNDEKGHGTHTASTAAGAFVEDAEALGNAKGKAAGI 241

Query: 241 APLAHLAMYKVCSPKGCSSSDILAALDAAIHDGVDVLSLSLGAPSRPFYIDNIAIGAFAA 300
           APLAHLA+YKVCS K C SSD+ A +DAAI DGVDVLS+SLG+ S PF+ DNIA+  F A
Sbjct: 242 APLAHLAIYKVCSGKRCPSSDVFAGIDAAIDDGVDVLSISLGSRSVPFFKDNIAVATFGA 301

Query: 301 IKMGIFVSCSAGNSGPSKSTLANEAPWILTVGASTIDRTIMALARLGNGEVHVGESLFQP 360
           I+ GIFVS SAGNSGP  STL+N+APWILTVGASTI+R I+A+A+LGNGE + GESL+QP
Sbjct: 302 IQKGIFVSSSAGNSGPLNSTLSNDAPWILTVGASTINRRIVAVAKLGNGEQYEGESLYQP 361

Query: 361 RDFPSSFLPLVYAGSSGVKGSEFCVQGSLTNLNVKGKMVVCERGGGIGRIAKGMVVKNAG 420
            DFPS FLPLVYAG+   K   FC +GSL N++VKGK+VVCE  GG+GR+AKG+VVKNAG
Sbjct: 362 NDFPSKFLPLVYAGNRENKTYAFCGEGSLENMDVKGKVVVCEGKGGVGRVAKGLVVKNAG 421

Query: 421 GAAMIIINQKADEFSTLAEAHILPATHVSYEAGLKIKSYIRSSQNPMASISFEGTLIGSR 480
           GAAMI+INQ+ D FSTL+EAH+LPATHVSY+AG+ IKSYI SSQNP ASISF+GT+IG  
Sbjct: 422 GAAMILINQEEDGFSTLSEAHVLPATHVSYKAGVLIKSYINSSQNPTASISFKGTVIGDG 481

Query: 481 ATTFSPAMASFSSRGPCLPSPGILKPDITGPGVNILAAWPFSLDNNTNKKSTFNVISGTS 540
               +P+MASFSSRGPCLPSPGILKPDITGPGVNILAAWPF LDN+TN KSTFNVISGTS
Sbjct: 482 DDFSAPSMASFSSRGPCLPSPGILKPDITGPGVNILAAWPFPLDNDTNTKSTFNVISGTS 541

Query: 541 MSCPHLSGIAALIKSVHPNWSPAAIKSAIMTSADVRNRQGRPIVDQDLKPANFFAMGSGH 600
           MSCPHLSGIAALIKSVHPNWSPAAIKSAIMT+A+++  QG PIVDQDL+PANFFAMG+GH
Sbjct: 542 MSCPHLSGIAALIKSVHPNWSPAAIKSAIMTTANIKTPQGEPIVDQDLQPANFFAMGAGH 601

Query: 601 VNPSKAADPGLVYDIQPDDYIPYLCGLYSDAQVSIIVRRKVTCSTISRIREGDLNYPSFA 660
           VNPSKAADPGLVYDIQPDDYIPYLCGLY D +VSIIV R V C  +  IREGDLNYPSF+
Sbjct: 602 VNPSKAADPGLVYDIQPDDYIPYLCGLYKDEEVSIIVHRTVICGLVPSIREGDLNYPSFS 661

Query: 661 VTLGPSQMFNRTVTNVGDANSIYSAIVEAPIGVSVKVTPNNLKFSRVIEKVTYSVTFSRD 720
           V LG  Q F RTVTNVG+ANS+Y+AIVEAP+GVS+ V P  L FSRV + +T++VTF+R 
Sbjct: 662 VALGGLQTFKRTVTNVGEANSVYTAIVEAPLGVSMTVMPRKLFFSRVNQTMTFTVTFNRI 721

Query: 721 DLVRTTSEFSEGYLIWV--SNKHMVRSPISVK 748
             V+   EF EGYL WV  S K++VRSP+SVK
Sbjct: 722 RWVKIVGEFGEGYLKWVSKSKKYVVRSPVSVK 753

BLAST of Bhi12G000322 vs. ExPASy TrEMBL
Match: A0A1S3AT16 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103482683 PE=3 SV=1)

HSP 1 Score: 1243.8 bits (3217), Expect = 0.0e+00
Identity = 628/751 (83.62%), Postives = 685/751 (91.21%), Query Frame = 0

Query: 3   LPFFCIFFLLNFHGIVAMKSELSGTSNQIKLQTYIVHVKESELRILGDAIDLESWYTSFL 62
           LPF CIFF LNFHG VAMK+ELS TSN   LQTYIVHVK+  L ILGD IDL++WYTSFL
Sbjct: 4   LPFICIFFFLNFHGYVAMKTELSSTSN-TNLQTYIVHVKQPVLEILGDTIDLQNWYTSFL 63

Query: 63  PETIK-TSNEQSRLLYSFRNVMSGFSARLT-EEVKAMEEKEGFISARPETILNLHTTHTP 122
           PETI+ +S+EQ RLLYS+R+VMSGFSARLT E+VKAMEEK+GFISA PET LNLHTTHTP
Sbjct: 64  PETIEASSDEQPRLLYSYRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTP 123

Query: 123 EYLGLNQQFGLWKDSNFGKGVIIGVLDTGISPDHPSFNDDGMPQPPAKWKGRCEFGASIC 182
           EYLGLN+ FGLWK+SNFGKGVIIGVLDTGI P+HPSFND+GM  PPAKWKGRCEFGASIC
Sbjct: 124 EYLGLNKHFGLWKNSNFGKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASIC 183

Query: 183 NNKLIGARTFNRANSALMGESPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGMAPL 242
           NNKLIGARTFN AN+ ++G+SP DENGHGTHTASTAAGTFV+GAEALGNARGKAVGMAPL
Sbjct: 184 NNKLIGARTFNLANNFVIGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPL 243

Query: 243 AHLAMYKVCSPKGCSSSDILAALDAAIHDGVDVLSLSLGAPSRPFYIDNIAIGAFAAIKM 302
           AH+AMYKVCSP GCSSSDILAALDAAI DGVDVLSLSLGAPS PF+ D IA+GAFAAIK 
Sbjct: 244 AHIAMYKVCSPNGCSSSDILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAMGAFAAIKK 303

Query: 303 GIFVSCSAGNSGPSKSTLANEAPWILTVGASTIDRTIMALARLGNGEVHVGESLFQPRDF 362
           GIFVSCSAGNSGPSK+TLANEAPWILTVGASTIDR I+ALA+LG+GEV+ GESLFQPR+F
Sbjct: 304 GIFVSCSAGNSGPSKNTLANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNF 363

Query: 363 PSSFLPLVYAGSSGVKGSEFCVQGSLTNLNVKGKMVVCERGGGIGRIAKGMVVKNAGGAA 422
           PS FLPLVYAG SG++GSE+C+QGSL  LNV GK+VVCERGGGI R+AKG+VVKN GGAA
Sbjct: 364 PSKFLPLVYAGKSGIEGSEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGGAA 423

Query: 423 MIIINQKADEFSTLAEAHILPATHVSYEAGLKIKSYIRSSQNPMASISFEGTLIGSRATT 482
           MI+INQK + FSTLAEAH+LP TH+SYE GLKIK+YI SS NP ASISF+GTL+G+RATT
Sbjct: 424 MILINQKPEGFSTLAEAHVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRATT 483

Query: 483 FSPAMASFSSRGPCLPSPGILKPDITGPGVNILAAWPFSLDN--NTNKKSTFNVISGTSM 542
           FSPAMASFSSRGPC  SPGILKPDITGPGVNILAAWPF L+N  NTN KSTFNVISGTSM
Sbjct: 484 FSPAMASFSSRGPCQASPGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSM 543

Query: 543 SCPHLSGIAALIKSVHPNWSPAAIKSAIMTSADVRNRQGRPIVDQDLKPANFFAMGSGHV 602
           SCPHLSGIAALIKS HPNWSPAAIKSAIMTSADVRN QG+PI+DQDLKPANFFAMGSGHV
Sbjct: 544 SCPHLSGIAALIKSYHPNWSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSGHV 603

Query: 603 NPSKAADPGLVYDIQPDDYIPYLCGLYSDAQVSIIVRRKVTCSTISRIREGDLNYPSFAV 662
           NPSKAA+PGLVYDIQPDDYIPYLC LY+DAQVSIIVR++VTCST+ RIREGDLNYPSFAV
Sbjct: 604 NPSKAANPGLVYDIQPDDYIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSFAV 663

Query: 663 TLGP-SQMFNRTVTNVGDANSIYSAIVEAPIGVSVKVTPNNLKFSRVIEKVTYSVTFSRD 722
           +LG  SQ FNRTVTNVGDANS+Y  IVEAP GVSVKVTP+NLKFS++ EKVTYSVTFSR 
Sbjct: 664 SLGAGSQTFNRTVTNVGDANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFSRI 723

Query: 723 DLVRTTSEFSEGYLIWVSNKHMVRSPISVKL 749
           D VRTTSEFSEGYLIWVS K MVRSPISVKL
Sbjct: 724 DFVRTTSEFSEGYLIWVSKKLMVRSPISVKL 753

BLAST of Bhi12G000322 vs. ExPASy TrEMBL
Match: A0A0A0KMQ7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G157280 PE=3 SV=1)

HSP 1 Score: 1219.9 bits (3155), Expect = 0.0e+00
Identity = 615/734 (83.79%), Postives = 670/734 (91.28%), Query Frame = 0

Query: 20  MKSELSGTSNQIKLQTYIVHVKESELRILGDAIDLESWYTSFLPETIK-TSNEQSRLLYS 79
           MK+EL   SN   LQTYIVHVK+ E+ ILGD IDL++WYTSFLPETI+ +SNEQSRLLYS
Sbjct: 1   MKTELWSASN-TNLQTYIVHVKQPEVEILGDTIDLQNWYTSFLPETIEASSNEQSRLLYS 60

Query: 80  FRNVMSGFSARLT-EEVKAMEEKEGFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNF 139
           +R+V+SGFSARLT E+VK MEEK+GFISA PET LNLHTTHTPEYLGLNQ FGLWK+SNF
Sbjct: 61  YRHVISGFSARLTKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNF 120

Query: 140 GKGVIIGVLDTGISPDHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFNRANSAL 199
           GKGVIIGVLDTGI P+HPSFND+GMP PPAKWKGRCEFGASICNNKLIGARTFN AN+  
Sbjct: 121 GKGVIIGVLDTGIHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVS 180

Query: 200 MGESPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGMAPLAHLAMYKVCSPKGCSSS 259
           +G+SP DENGHGTHTASTAAGTFV+GAEALGNARGKAVGMAPLAH+A+YKVCSPKGCSSS
Sbjct: 181 IGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSS 240

Query: 260 DILAALDAAIHDGVDVLSLSLGAPSRPFYIDNIAIGAFAAIKMGIFVSCSAGNSGPSKST 319
           DILAALDAAI DGVDVLSLSLGAPS PF+ D IA+GAFAAIK GIFVSCSAGNSGPSK+T
Sbjct: 241 DILAALDAAIDDGVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNT 300

Query: 320 LANEAPWILTVGASTIDRTIMALARLGNGEVHVGESLFQPRDFPSSFLPLVYAGSSGVKG 379
           LANEAPWILTVGASTIDR I+ALA+L +G+V  GESLFQPRDF S FLPLVYAG SG++G
Sbjct: 301 LANEAPWILTVGASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEG 360

Query: 380 SEFCVQGSLTNLNVKGKMVVCERGGGIGRIAKGMVVKNAGGAAMIIINQKADEFSTLAEA 439
           SE+CV+GSL  LNV GK+VVCERGGGIGRIAKG+VVKN GGAAMI++NQK D FSTLAEA
Sbjct: 361 SEYCVEGSLEKLNVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEA 420

Query: 440 HILPATHVSYEAGLKIKSYIRSSQNPMASISFEGTLIGSRATTFSPAMASFSSRGPCLPS 499
           H+LP TH+SYE GLKIK YI SS NP ASISFEGTL+G+RATTFSPAMASFSSRGPC  S
Sbjct: 421 HVLPTTHLSYEDGLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQAS 480

Query: 500 PGILKPDITGPGVNILAAWPFSLDN--NTNKKSTFNVISGTSMSCPHLSGIAALIKSVHP 559
           PGILKPDITGPGVNILAAWPF L+N  NTN KSTFNVISGTSMSCPHLSGIAALIKS HP
Sbjct: 481 PGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHP 540

Query: 560 NWSPAAIKSAIMTSADVRNRQGRPIVDQDLKPANFFAMGSGHVNPSKAADPGLVYDIQPD 619
           NWSPAAIKSAIMTSADVRN QG+PIVDQDLKPANFFAMGSGHVNPSKAA+PGLVYDIQPD
Sbjct: 541 NWSPAAIKSAIMTSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPD 600

Query: 620 DYIPYLCGLYSDAQVSIIVRRKVTCSTISRIREGDLNYPSFAVTLG-PSQMFNRTVTNVG 679
           DY+PYLC LY+DAQVSIIVRR+VTCST+SRIREGDLNYPSFAV+LG  SQ FNRTVTNVG
Sbjct: 601 DYVPYLCHLYTDAQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVG 660

Query: 680 DANSIYSAIVEAPIGVSVKVTPNNLKFSRVIEKVTYSVTFSRDDLVRTTSEFSEGYLIWV 739
           DANS+Y AIV+AP GVSV+VTP NLKFS++ EK+TYSVTFSR D VRT SEFSEGYLIWV
Sbjct: 661 DANSVYYAIVKAPAGVSVRVTPRNLKFSKLNEKLTYSVTFSRIDFVRTRSEFSEGYLIWV 720

Query: 740 SNKHMVRSPISVKL 749
           SNKH+VRSPISVKL
Sbjct: 721 SNKHIVRSPISVKL 733

BLAST of Bhi12G000322 vs. ExPASy TrEMBL
Match: A0A5A7THE4 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003290 PE=3 SV=1)

HSP 1 Score: 1213.7 bits (3139), Expect = 0.0e+00
Identity = 614/734 (83.65%), Postives = 669/734 (91.14%), Query Frame = 0

Query: 20  MKSELSGTSNQIKLQTYIVHVKESELRILGDAIDLESWYTSFLPETIK-TSNEQSRLLYS 79
           MK+ELS TSN   LQTYIVHVK+  L ILGD IDL++WYTSFLPETI+ +S+EQ RLLYS
Sbjct: 1   MKTELSSTSN-TNLQTYIVHVKQPVLEILGDTIDLQNWYTSFLPETIEASSDEQPRLLYS 60

Query: 80  FRNVMSGFSARLT-EEVKAMEEKEGFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNF 139
           +R+VMSGFSARLT E+VKAMEEK+GFISA PET LNLHTTHTPEYLGLN  FGLWK+SNF
Sbjct: 61  YRHVMSGFSARLTKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNTHFGLWKNSNF 120

Query: 140 GKGVIIGVLDTGISPDHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFNRANSAL 199
           GKGVIIGVLDTGI P+HPSFND+GM  PPAKWKGRCEFGASICNNKLIGARTFN AN+ +
Sbjct: 121 GKGVIIGVLDTGIHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNFV 180

Query: 200 MGESPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGMAPLAHLAMYKVCSPKGCSSS 259
           +G+SP DENGHGTHTASTAAGTFV+GAEALGNARGKAVGMAPLAH+AMYKVCSP GCSSS
Sbjct: 181 IGKSPNDENGHGTHTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSS 240

Query: 260 DILAALDAAIHDGVDVLSLSLGAPSRPFYIDNIAIGAFAAIKMGIFVSCSAGNSGPSKST 319
           DILAALDAAI DGVDVLSLSLGAPS PF+ D IAIGAFAAIK GIFVSCSAGNSGPSK+T
Sbjct: 241 DILAALDAAIDDGVDVLSLSLGAPSTPFFRDTIAIGAFAAIKKGIFVSCSAGNSGPSKNT 300

Query: 320 LANEAPWILTVGASTIDRTIMALARLGNGEVHVGESLFQPRDFPSSFLPLVYAGSSGVKG 379
           LANEAPWILTVGASTIDR I+ALA+LG+GEV+ GESLFQPR+FPS FLPLVYAG SG++G
Sbjct: 301 LANEAPWILTVGASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIEG 360

Query: 380 SEFCVQGSLTNLNVKGKMVVCERGGGIGRIAKGMVVKNAGGAAMIIINQKADEFSTLAEA 439
           SE+C+QGSL  LNV GK+VVCERGGGI R+AKG+VVKN GGAAMI+INQK + FSTLAEA
Sbjct: 361 SEYCIQGSLEKLNVTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAEA 420

Query: 440 HILPATHVSYEAGLKIKSYIRSSQNPMASISFEGTLIGSRATTFSPAMASFSSRGPCLPS 499
           H+LP TH+SYE GLKIK+YI SS NP ASISF+GTL+G+RATTFSPAMASFSSRGPC  S
Sbjct: 421 HVLPTTHLSYEDGLKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQAS 480

Query: 500 PGILKPDITGPGVNILAAWPFSLDN--NTNKKSTFNVISGTSMSCPHLSGIAALIKSVHP 559
           PGILKPDITGPGVNILAAWPF L+N  NTN KSTFNVISGTSMSCPHLSGIAALIKS HP
Sbjct: 481 PGILKPDITGPGVNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYHP 540

Query: 560 NWSPAAIKSAIMTSADVRNRQGRPIVDQDLKPANFFAMGSGHVNPSKAADPGLVYDIQPD 619
           NWSPAAIKSAIMTSADVRN QG+PI+DQDLKPANFFAMGSGHVNPSKAA+PGLVYDIQPD
Sbjct: 541 NWSPAAIKSAIMTSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPD 600

Query: 620 DYIPYLCGLYSDAQVSIIVRRKVTCSTISRIREGDLNYPSFAVTL-GPSQMFNRTVTNVG 679
           DYIPYLC LY+DAQVSIIVR++VTCST+ RIREGDLNYPSFAV+L   SQ FNRTVTNVG
Sbjct: 601 DYIPYLCHLYTDAQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLEAGSQTFNRTVTNVG 660

Query: 680 DANSIYSAIVEAPIGVSVKVTPNNLKFSRVIEKVTYSVTFSRDDLVRTTSEFSEGYLIWV 739
           DANS+Y  IVEAP GVSVKVTP+NLKFS++ EKVTYSVTFSR D VRTTSEFSEGYLIWV
Sbjct: 661 DANSVYYPIVEAPAGVSVKVTPSNLKFSKLNEKVTYSVTFSRIDFVRTTSEFSEGYLIWV 720

Query: 740 SNKHMVRSPISVKL 749
           S K MVRSPISVKL
Sbjct: 721 SKKLMVRSPISVKL 733

BLAST of Bhi12G000322 vs. ExPASy TrEMBL
Match: A0A5A7TMM4 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003280 PE=3 SV=1)

HSP 1 Score: 1087.0 bits (2810), Expect = 0.0e+00
Identity = 544/752 (72.34%), Postives = 636/752 (84.57%), Query Frame = 0

Query: 1   MVLPFFCIFFLLNFHGIVAMKSELSGTSNQIKLQTYIVHVKESELRILGDAID--LESWY 60
           +VL F  IFFLLN    VA K EL  T  Q KLQTYIVHV++ ++R++G++ +  +ESWY
Sbjct: 2   VVLRFLSIFFLLNIESHVAAKVELPTTPLQSKLQTYIVHVEQPKIRLVGESSNDHIESWY 61

Query: 61  TSFLPETIKTSNEQSRLLYSFRNVMSGFSARLT-EEVKAMEEKEGFISARPETILNLHTT 120
            SF+P++ +T+ EQ +LLYS+RNVMSGFSARLT E+VKAME+K+GFISA PETI++LHTT
Sbjct: 62  MSFIPKSTETTVEQPQLLYSYRNVMSGFSARLTIEQVKAMEKKDGFISAMPETIMSLHTT 121

Query: 121 HTPEYLGLNQQFGLWKDSNFGKGVIIGVLDTGISPDHPSFNDDGMPQPPAKWKGRCEFGA 180
           HTPEYLGLNQQFG+WK+SNFGKGVI+GVLDTGI P+HPSFND+GM  PPAKWKG+CEF +
Sbjct: 122 HTPEYLGLNQQFGIWKNSNFGKGVIVGVLDTGIHPNHPSFNDEGMSPPPAKWKGKCEFNS 181

Query: 181 SICNNKLIGARTFNRANSALMGESPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGM 240
           S+CNNKLIGARTFN  N+ LM ESP DE GHGTHTASTAAG FVE AEALGNA+GKA G+
Sbjct: 182 SLCNNKLIGARTFNLGNNFLMEESPNDEKGHGTHTASTAAGAFVEDAEALGNAKGKAAGI 241

Query: 241 APLAHLAMYKVCSPKGCSSSDILAALDAAIHDGVDVLSLSLGAPSRPFYIDNIAIGAFAA 300
           APLAHLA+YKVCS K C SSD+ A +DAAI DGVDVLS+SLG+ S PF+ DNIA+  F A
Sbjct: 242 APLAHLAIYKVCSGKRCPSSDVFAGIDAAIDDGVDVLSISLGSRSVPFFKDNIAVATFGA 301

Query: 301 IKMGIFVSCSAGNSGPSKSTLANEAPWILTVGASTIDRTIMALARLGNGEVHVGESLFQP 360
           I+ GIFVS SAGNSGP  STL+N+APWILTVGASTI+R I+A+A+LGNGE + GESL+QP
Sbjct: 302 IQKGIFVSSSAGNSGPLNSTLSNDAPWILTVGASTINRRIVAVAKLGNGEQYEGESLYQP 361

Query: 361 RDFPSSFLPLVYAGSSGVKGSEFCVQGSLTNLNVKGKMVVCERGGGIGRIAKGMVVKNAG 420
            DFPS FLPLVYAG+   K   FC +GSL N++VKGK+VVCE  GG+GR+AKG+VVKNAG
Sbjct: 362 NDFPSKFLPLVYAGNRENKTYAFCGEGSLENMDVKGKVVVCEGKGGVGRVAKGLVVKNAG 421

Query: 421 GAAMIIINQKADEFSTLAEAHILPATHVSYEAGLKIKSYIRSSQNPMASISFEGTLIGSR 480
           GAAMI+INQ+ D FSTL+EAH+LPATHVSY+AG+ IKSYI SSQNP ASISF+GT+IG  
Sbjct: 422 GAAMILINQEEDGFSTLSEAHVLPATHVSYKAGVLIKSYINSSQNPTASISFKGTVIGDG 481

Query: 481 ATTFSPAMASFSSRGPCLPSPGILKPDITGPGVNILAAWPFSLDNNTNKKSTFNVISGTS 540
               +P+MASFSSRGPCLPSPGILKPDITGPGVNILAAWPF LDN+TN KSTFNVISGTS
Sbjct: 482 DDFSAPSMASFSSRGPCLPSPGILKPDITGPGVNILAAWPFPLDNDTNTKSTFNVISGTS 541

Query: 541 MSCPHLSGIAALIKSVHPNWSPAAIKSAIMTSADVRNRQGRPIVDQDLKPANFFAMGSGH 600
           MSCPHLSGIAALIKSVHPNWSPAAIKSAIMT+A+++  QG PIVDQDL+PANFFAMG+GH
Sbjct: 542 MSCPHLSGIAALIKSVHPNWSPAAIKSAIMTTANIKTPQGEPIVDQDLQPANFFAMGAGH 601

Query: 601 VNPSKAADPGLVYDIQPDDYIPYLCGLYSDAQVSIIVRRKVTCSTISRIREGDLNYPSFA 660
           VNPSKAADPGLVYDIQPDDYIPYLCGLY D +VSIIV R V C  +  IREGDLNYPSF+
Sbjct: 602 VNPSKAADPGLVYDIQPDDYIPYLCGLYKDEEVSIIVHRTVICGLVPSIREGDLNYPSFS 661

Query: 661 VTLGPSQMFNRTVTNVGDANSIYSAIVEAPIGVSVKVTPNNLKFSRVIEKVTYSVTFSRD 720
           V LG  Q F RTVTNVG+ANS+Y+AIVEAP+GVS+ V P  L FSRV + +T++VTF+R 
Sbjct: 662 VALGGLQTFKRTVTNVGEANSVYTAIVEAPLGVSMTVMPRKLFFSRVNQTMTFTVTFNRI 721

Query: 721 DLVRTTSEFSEGYLIWV--SNKHMVRSPISVK 748
             V+   EF EGYL WV  S K++VRSP+SVK
Sbjct: 722 RWVKIVGEFGEGYLKWVSKSKKYVVRSPVSVK 753

BLAST of Bhi12G000322 vs. ExPASy TrEMBL
Match: A0A1S3AUP1 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103483066 PE=3 SV=1)

HSP 1 Score: 1080.9 bits (2794), Expect = 0.0e+00
Identity = 539/744 (72.45%), Postives = 630/744 (84.68%), Query Frame = 0

Query: 9   FFLLNFHGIVAMKSELSGTSNQIKLQTYIVHVKESELRILGDAID--LESWYTSFLPETI 68
           FFLLN    VA K EL  T  Q KLQTYIVHV++ ++R++G++ +  +ESWY SF+P++ 
Sbjct: 19  FFLLNIESHVAAKVELPTTPLQSKLQTYIVHVEQPKIRLVGESSNDHIESWYMSFIPKST 78

Query: 69  KTSNEQSRLLYSFRNVMSGFSARLT-EEVKAMEEKEGFISARPETILNLHTTHTPEYLGL 128
           +T+ EQ +LLYS+RNVMSGFSARLT E+VKAME+K+GFISA PETI++LHTTHTPEYLGL
Sbjct: 79  ETTVEQPQLLYSYRNVMSGFSARLTIEQVKAMEKKDGFISAMPETIMSLHTTHTPEYLGL 138

Query: 129 NQQFGLWKDSNFGKGVIIGVLDTGISPDHPSFNDDGMPQPPAKWKGRCEFGASICNNKLI 188
           NQQFG+WK+SNFGKGVI+GVLDTGI P+HPSFND+GM  PPAKWKG+CEF +S+CNNKLI
Sbjct: 139 NQQFGIWKNSNFGKGVIVGVLDTGIHPNHPSFNDEGMSPPPAKWKGKCEFNSSLCNNKLI 198

Query: 189 GARTFNRANSALMGESPIDENGHGTHTASTAAGTFVEGAEALGNARGKAVGMAPLAHLAM 248
           GARTFN  N+ LM ESP DE GHGTHTASTAAG FVE AEALGNA+GKA G+APLAHLA+
Sbjct: 199 GARTFNLGNNFLMEESPNDEKGHGTHTASTAAGAFVEDAEALGNAKGKAAGIAPLAHLAI 258

Query: 249 YKVCSPKGCSSSDILAALDAAIHDGVDVLSLSLGAPSRPFYIDNIAIGAFAAIKMGIFVS 308
           YKVCS K C SSD+ A +DAAI DGVDVLS+SLG+ S PF+ DNIA+  F AI+ GIFVS
Sbjct: 259 YKVCSGKRCPSSDVFAGIDAAIDDGVDVLSISLGSRSVPFFKDNIAVATFGAIQKGIFVS 318

Query: 309 CSAGNSGPSKSTLANEAPWILTVGASTIDRTIMALARLGNGEVHVGESLFQPRDFPSSFL 368
            SAGNSGP  STL+N+APWILTVGASTI+R I+A+A+LGNGE + GESL+QP DFPS FL
Sbjct: 319 SSAGNSGPLNSTLSNDAPWILTVGASTINRRIVAVAKLGNGEQYEGESLYQPNDFPSKFL 378

Query: 369 PLVYAGSSGVKGSEFCVQGSLTNLNVKGKMVVCERGGGIGRIAKGMVVKNAGGAAMIIIN 428
           PLVYAG+   K   FC +GSL N++VKGK+VVCE  GG+GR+AKG+VVKNAGGAAMI+IN
Sbjct: 379 PLVYAGNRENKTYAFCGEGSLENMDVKGKVVVCEGKGGVGRVAKGLVVKNAGGAAMILIN 438

Query: 429 QKADEFSTLAEAHILPATHVSYEAGLKIKSYIRSSQNPMASISFEGTLIGSRATTFSPAM 488
           Q+ D FSTL+EAH+LPATHVSY+AG+ IKSYI SSQNP ASISF+GT+IG      +P+M
Sbjct: 439 QEEDGFSTLSEAHVLPATHVSYKAGVLIKSYINSSQNPTASISFKGTVIGDGDDFSAPSM 498

Query: 489 ASFSSRGPCLPSPGILKPDITGPGVNILAAWPFSLDNNTNKKSTFNVISGTSMSCPHLSG 548
           ASFSSRGPCLPSPGILKPDITGPGVNILAAWPF LDN+TN KSTFNVISGTSMSCPHLSG
Sbjct: 499 ASFSSRGPCLPSPGILKPDITGPGVNILAAWPFPLDNDTNTKSTFNVISGTSMSCPHLSG 558

Query: 549 IAALIKSVHPNWSPAAIKSAIMTSADVRNRQGRPIVDQDLKPANFFAMGSGHVNPSKAAD 608
           IAALIKSVHPNWSPAAIKSAIMT+A+++  QG PI DQDL+PANFFAMG+GHVNPSKAAD
Sbjct: 559 IAALIKSVHPNWSPAAIKSAIMTTANIKTPQGEPITDQDLQPANFFAMGAGHVNPSKAAD 618

Query: 609 PGLVYDIQPDDYIPYLCGLYSDAQVSIIVRRKVTCSTISRIREGDLNYPSFAVTLGPSQM 668
           PGLVYDIQPDDYIPYLCGLY D +VSIIV R V C  +  IREGDLNYPSF+V LG  Q 
Sbjct: 619 PGLVYDIQPDDYIPYLCGLYKDEEVSIIVHRTVICGLVLSIREGDLNYPSFSVALGGLQT 678

Query: 669 FNRTVTNVGDANSIYSAIVEAPIGVSVKVTPNNLKFSRVIEKVTYSVTFSRDDLVRTTSE 728
           F RTVTNVG+ANS+Y+AIVEAP+GVS+ V P  L FSRV + +T++VTF+R   V+   E
Sbjct: 679 FKRTVTNVGEANSVYTAIVEAPLGVSMTVMPRKLFFSRVNQTMTFTVTFNRIRWVKIVGE 738

Query: 729 FSEGYLIWV--SNKHMVRSPISVK 748
           F EGYL WV  S K++VRSP+SVK
Sbjct: 739 FGEGYLKWVSKSKKYVVRSPVSVK 762