Bhi12G000115 (gene) Wax gourd (B227) v1

Overview


Name	Bhi12G000115
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	RNase H domain-containing protein
Location	chr12: 3885874 .. 3886310 (-)
RNA-Seq Expression	Bhi12G000115
Synteny	Bhi12G000115

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCGAAGTGTACCAAAAGTCTGGTTGCCTCCTCCTCCAAACCGCTTCAAGATTAATACTGATGCCGCTTGGACTTTTGATTTCTCTTCGATTGGCTTAGGAATTACCGAAAGGTCGTTCGACGGATCGATTATTGCTATTTCAACAATCCATACTCAGAATATCCCTAATGTTTCTCTTGCTGAACCCCTTGCAAATAAGGAAGGTCCGAAGCTAGCAATCACCTTGAAATGCCAAAATGTCATCCTGGAATCTGATTGCATGTCTGTGATTAATCTTCTTCAAGGGAAATCCTCTAGCATTGTCGAAATTGAATGTTGTATAGACGATATTTGCTCACTCCAAAGTAGCTTTACATCATCCTTATTCTCGTTTGCTCCTCGTTCATCCAATGCCCTAGCTGATTGTGTTGCTAAATTTGTGAAGAAAGATCCGTAA

mRNA sequence

ATCGAAGTGTACCAAAAGTCTGGTTGCCTCCTCCTCCAAACCGCTTCAAGATTAATACTGATGCCGCTTGGACTTTTGATTTCTCTTCGATTGGCTTAGGAATTACCGAAAGGTCGTTCGACGGATCGATTATTGCTATTTCAACAATCCATACTCAGAATATCCCTAATGTTTCTCTTGCTGAACCCCTTGCAAATAAGGAAGGTCCGAAGCTAGCAATCACCTTGAAATGCCAAAATGTCATCCTGGAATCTGATTGCATGTCTGTGATTAATCTTCTTCAAGGGAAATCCTCTAGCATTGTCGAAATTGAATGTTGTATAGACGATATTTGCTCACTCCAAAGTAGCTTTACATCATCCTTATTCTCGTTTGCTCCTCGTTCATCCAATGCCCTAGCTGATTGTGTTGCTAAATTTGTGAAGAAAGATCCGTAA

Coding sequence (CDS)

ATCGAAGTGTACCAAAAGTCTGGTTGCCTCCTCCTCCAAACCGCTTCAAGATTAATACTGATGCCGCTTGGACTTTTGATTTCTCTTCGATTGGCTTAGGAATTACCGAAAGGTCGTTCGACGGATCGATTATTGCTATTTCAACAATCCATACTCAGAATATCCCTAATGTTTCTCTTGCTGAACCCCTTGCAAATAAGGAAGGTCCGAAGCTAGCAATCACCTTGAAATGCCAAAATGTCATCCTGGAATCTGATTGCATGTCTGTGATTAATCTTCTTCAAGGGAAATCCTCTAGCATTGTCGAAATTGAATGTTGTATAGACGATATTTGCTCACTCCAAAGTAGCTTTACATCATCCTTATTCTCGTTTGCTCCTCGTTCATCCAATGCCCTAGCTGATTGTGTTGCTAAATTTGTGAAGAAAGATCCGTAA

Protein sequence

RSVPKVWLPPPPNRFKINTDAAWTFDFSSIGLGITERSFDGSIIAISTIHTQNIPNVSLAEPLANKEGPKLAITLKCQNVILESDCMSVINLLQGKSSSIVEIECCIDDICSLQSSFTSSLFSFAPRSSNALADCVAKFVKKDP

Homology

[Close]

BLAST of Bhi12G000115 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 53.5 bits (127), Expect = 1.6e-07
Identity = 38/138 (27.54%), Postives = 59/138 (42.75%), Query Frame = 0


Query: 1   RSVPKVWLPPPPNRFKINTDAAWTFDFSSIGLGITERSFDGSIIAISTIHTQNIPNVSLA 60
           R++   W  PP    K NTDA W  +    G+G   R+  G ++ +         NV  A
Sbjct: 131 RNLSVQWKAPPYQWVKCNTDATWQLENPRCGIGWILRNESGGVLWMGARALPRTKNVLEA 190

Query: 61  EPLANKEGPKLAITLKCQNVILESDCMSVINLLQGKSSSIVEIECCIDDICSLQSSFTSS 120
           E  A +           + +I ESD  +++NLL         ++  ++DI  L   F   
Sbjct: 191 ELEALRWAVLTMSRFNYKRIIFESDAQALVNLL-NSDDFWPTLQPALEDIQQLLHHFEEV 250

Query: 121 LFSFAPRSSNALADCVAK 139
            F F PR  N +AD +A+
Sbjct: 251 KFEFTPRGGNKVADRIAR 267

[Close]

BLAST of Bhi12G000115 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 53.1 bits (126), Expect = 2.1e-07
Identity = 44/142 (30.99%), Postives = 60/142 (42.25%), Query Frame = 0


Query: 7   WLPPPPNRFKINTDAAWTFDFSSIGLGITERSFDGSIIAISTIHTQNIPNVSLAEPLANK 66
           W PPP    K NTDA W  D    G+G   R+  G +  +       + +V  AE  A +
Sbjct: 420 WRPPPHQWVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARALPKLKSVLEAELEAMR 479

Query: 67  EGPKLAITLKCQNVILESDCMSVINLLQGKSSSIVEIECCIDDICSLQSSFTSSLFSFAP 126
                    +   VI ESD   +I +L         ++  I D+  L S FT   F F P
Sbjct: 480 WAVLSLSRFQYNYVIFESDSQVLIEIL-NNDEIWPSLKPTIQDLQRLLSQFTEVKFVFIP 539

Query: 127 RSSNALADCVAK----FVKKDP 145
           R  N LA+ VA+    F+  DP
Sbjct: 540 REGNTLAERVARESLSFLNYDP 560

[Close]

BLAST of Bhi12G000115 vs. TAIR 10
Match: AT4G09490.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 46.6 bits (109), Expect = 2.0e-05
Identity = 41/135 (30.37%), Postives = 62/135 (45.93%), Query Frame = 0


Query: 8   LPPPPNRFKINTDAAWTFDFSSIGLGITERSFDGSIIAISTIHTQ----NIPNVSLAEPL 67
           L P  +R  I TDAAW  +   +G G   R+       ++ +H Q    N+    +AE +
Sbjct: 24  LRPDLDRVTIFTDAAWKHETKDVGFGWVIRNCP----ELTALHYQSAARNVRLPLMAEAI 83

Query: 68  ANKEGPKLAITLKCQNVILESDCMSVINLLQGKSSSIVEIECCIDDICSLQSSFTSSLFS 127
           A     + A ++    + + SD   +I  +  +S S  E    I DI +L   F    FS
Sbjct: 84  ALFLALQYAQSIGITKLSMASDSQQLITAITSESPS-TEFYGIIFDILNLSLGFADVSFS 143

Query: 128 FAPRSSNALADCVAK 139
           F PRS N +AD +AK
Sbjct: 144 FVPRSENRVADELAK 153

[Close]

BLAST of Bhi12G000115 vs. NCBI nr
Match: XP_024033146.1 (uncharacterized protein LOC112095446 [Citrus clementina])

HSP 1 Score: 80.1 bits (196), Expect = 1.7e-11
Identity = 50/132 (37.88%), Postives = 69/132 (52.27%), Query Frame = 0


Query: 7   WLPPPPNRFKINTDAAWTFDFSSIGLGITERSFDGSIIAISTIHTQNIPNVSLAEPLANK 66
           W PPP   +K+N DAA   +    GLG+  R+  G II  +   T+   NV+ AE  A K
Sbjct: 105 WFPPPVGFYKVNVDAAVHTEQQLTGLGVVIRNPQGQIIVAAVKSTKFQDNVTAAEAEAVK 164

Query: 67  EGPKLAITLKCQNVILESDCMSVINLLQGKSSSIVEIECCIDDICSLQSSFTSSLFSFAP 126
            G ++A+  +   VI+E+DC+ V NL   K+SS  EI   I DI S +  F S      P
Sbjct: 165 WGLEIALEARLATVIIETDCIEVANLANSKTSSRKEIMWTISDIHSYKEKFQSLSIQHVP 224

Query: 127 RSSNALADCVAK 139
           R  N+ A  +AK
Sbjct: 225 RCCNSCAHSLAK 236

[Close]

BLAST of Bhi12G000115 vs. NCBI nr
Match: XP_006491472.1 (uncharacterized protein LOC102626455 [Citrus sinensis])

HSP 1 Score: 79.7 bits (195), Expect = 2.2e-11
Identity = 48/137 (35.04%), Postives = 67/137 (48.91%), Query Frame = 0


Query: 7    WLPPPPNRFKINTDAAWTFDFSSIGLGITERSFDGSIIAISTIHTQNIPNVSLAEPLANK 66
            W PP  N  K+N DAA +     +GLG   R  +G I+A+     Q    VSLAE  A  
Sbjct: 1293 WKPPSQNVLKLNVDAAVSTKDQKVGLGAIVRDAEGKILAVGIKQAQFRERVSLAEAEAIH 1352

Query: 67   EGPKLAITLKCQNVILESDCMSVINLLQGKSSSIVEIECCIDDICSLQSSFTSSLFSFAP 126
             G ++A  +   ++I+ESDC  V+ LL     S  EI   + D+      F    FSF P
Sbjct: 1353 WGLQVANQISSSSLIVESDCKEVVELLNNTKGSRTEIHWILSDVRRESKEFKQVQFSFIP 1412

Query: 127  RSSNALADCVAKFVKKD 144
            R+ N  A  +AKF  ++
Sbjct: 1413 RTCNTYAHALAKFALRN 1429

[Close]

BLAST of Bhi12G000115 vs. NCBI nr
Match: XP_024046732.1 (uncharacterized protein LOC112101057 [Citrus clementina])

HSP 1 Score: 78.6 bits (192), Expect = 5.0e-11
Identity = 49/132 (37.12%), Postives = 69/132 (52.27%), Query Frame = 0


Query: 7   WLPPPPNRFKINTDAAWTFDFSSIGLGITERSFDGSIIAISTIHTQNIPNVSLAEPLANK 66
           W PPP   +K+N DAA   +    GLG+  R+  G +I  +   T+   NV+ AE  A K
Sbjct: 232 WCPPPVGFYKVNVDAAVHTEQQLTGLGVVIRNPQGQVIVTAVKSTKFQDNVTAAEAEAVK 291

Query: 67  EGPKLAITLKCQNVILESDCMSVINLLQGKSSSIVEIECCIDDICSLQSSFTSSLFSFAP 126
            G ++A+  +   VI+E+DC+ V NL   K+SS  EI   I DI S +  F S      P
Sbjct: 292 WGLEIALEARLAAVIIETDCIEVANLANSKTSSRKEIVWTISDIHSYKEKFQSLSIQHVP 351

Query: 127 RSSNALADCVAK 139
           R  N+ A  +AK
Sbjct: 352 RCCNSCAHSLAK 363

[Close]

BLAST of Bhi12G000115 vs. NCBI nr
Match: XP_015385025.1 (uncharacterized protein LOC107176703 [Citrus sinensis])

HSP 1 Score: 78.6 bits (192), Expect = 5.0e-11
Identity = 50/132 (37.88%), Postives = 69/132 (52.27%), Query Frame = 0


Query: 7   WLPPPPNRFKINTDAAWTFDFSSIGLGITERSFDGSIIAISTIHTQNIPNVSLAEPLANK 66
           W PPP   +K+N DAA   +    GLG+  R+  G II  +   T+   NV+ AE  A K
Sbjct: 105 WCPPPVGFYKVNVDAAVHTEQQLTGLGVVIRNPQGQIIVAAIKSTKFQDNVTAAEAEAVK 164

Query: 67  EGPKLAITLKCQNVILESDCMSVINLLQGKSSSIVEIECCIDDICSLQSSFTSSLFSFAP 126
            G ++A+  +   VI+E+DC+ V NL   K+SS  EI   I DI S +  F S      P
Sbjct: 165 WGLEIALEARLAAVIIETDCIEVANLANSKTSSKKEIMWTISDIHSYKEKFQSLSIQHVP 224

Query: 127 RSSNALADCVAK 139
           R  N+ A  +AK
Sbjct: 225 RCCNSCAHSLAK 236

[Close]

BLAST of Bhi12G000115 vs. NCBI nr
Match: KAF7814428.1 (cysteine desulfurase mitochondrial-like [Senna tora])

HSP 1 Score: 77.8 bits (190), Expect = 8.5e-11
Identity = 45/133 (33.83%), Postives = 69/133 (51.88%), Query Frame = 0


Query: 7   WLPPPPNRFKINTDAAWTFDFSSIGLGITERSFDGSIIAISTIHTQNIPNVSLAEPLANK 66
           W  P     K+N DA+   +    G+G   R+F G  +A      +NI +  L E  A  
Sbjct: 629 WEAPMWREMKVNVDAS-LLEQGKGGIGCVVRNFQGRCLAAFAKRMKNIESAELLEAFAFL 688

Query: 67  EGPKLAITLKCQNVILESDCMSVINLLQGKSSSIVEIECCIDDICSLQSSFTSSLFSFAP 126
           EG +LA  L+C N+ILE D   V++L+ G S ++  +   +DDI     +F+     + P
Sbjct: 689 EGLELAKNLRCDNIILEGDAKKVVDLIHGSSQNLSHLGMLVDDIRKGMKNFSRVKVQWVP 748

Query: 127 RSSNALADCVAKF 140
           R+SNA+AD +A F
Sbjct: 749 RNSNAVADRIANF 760

[Close]

BLAST of Bhi12G000115 vs. ExPASy TrEMBL
Match: A0A803P4U9 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 1.2e-10
Identity = 47/133 (35.34%), Postives = 67/133 (50.38%), Query Frame = 0


Query: 7    WLPPPPNRFKINTDAAWTFDFSSIGLGITERSFDGSIIAISTIHTQNIPNVSLAEPLANK 66
            W PPPP  F INTDA+        GLG+  R   G+++  +T       +V LAE +A +
Sbjct: 1676 WSPPPPGTFMINTDASLIEGQPGCGLGVIIRDHLGALVTATTEFIPGCLSVLLAEAMAIR 1735

Query: 67   EGPKLAITLKCQNVILESDCMSVINLLQGKSSSIVEIECCIDDICSLQSSFTSSLFSFAP 126
               KLA T   QN+ + SD  SVIN L+GK+    +    IDD         +  F F+ 
Sbjct: 1736 LALKLAETRLMQNIFIASDSQSVINALKGKTHINTDWGFVIDDCIQASKKLLNLSFIFSL 1795

Query: 127  RSSNALADCVAKF 140
            R  N++A C+A +
Sbjct: 1796 RKCNSVAHCIANW 1808

[Close]

BLAST of Bhi12G000115 vs. ExPASy TrEMBL
Match: A0A7J6GGV5 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_001227 PE=4 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 1.2e-10
Identity = 47/133 (35.34%), Postives = 67/133 (50.38%), Query Frame = 0


Query: 7   WLPPPPNRFKINTDAAWTFDFSSIGLGITERSFDGSIIAISTIHTQNIPNVSLAEPLANK 66
           W PPPP  F INTDA+        GLG+  R   G+++  +T       +V LAE +A +
Sbjct: 596 WSPPPPGTFMINTDASLIEGQPGCGLGVIIRDHLGALVTATTEFIPGCLSVLLAEAMAIR 655

Query: 67  EGPKLAITLKCQNVILESDCMSVINLLQGKSSSIVEIECCIDDICSLQSSFTSSLFSFAP 126
              KLA T   QN+ + SD  SVIN L+GK+    +    IDD         +  F F+ 
Sbjct: 656 LALKLAETRLMQNIFIASDSQSVINALKGKTHINTDWGFVIDDCIQASKKLLNLSFIFSL 715

Query: 127 RSSNALADCVAKF 140
           R  N++A C+A +
Sbjct: 716 RKCNSVAHCIANW 728

[Close]

BLAST of Bhi12G000115 vs. ExPASy TrEMBL
Match: A0A7J6FIX9 (RNase H domain-containing protein OS=Cannabis sativa OX=3483 GN=G4B88_026879 PE=4 SV=1)

HSP 1 Score: 75.9 bits (185), Expect = 1.6e-10
Identity = 48/133 (36.09%), Postives = 67/133 (50.38%), Query Frame = 0


Query: 7   WLPPPPNRFKINTDAAWTFDFSSIGLGITERSFDGSIIAISTIHTQNIPNVSLAEPLANK 66
           W PPPP  F INTDA+     S  GL +  R   G+++A +T       +V LAE LA +
Sbjct: 24  WSPPPPGTFMINTDASLIEGQSGCGLRVIIRDHLGALVAAATDFIPGCLSVLLAETLAIR 83

Query: 67  EGPKLAITLKCQNVILESDCMSVINLLQGKSSSIVEIECCIDDICSLQSSFTSSLFSFAP 126
              KL  T   Q + + SD  SVI  L+GK+    +    IDD       F +  F+F+P
Sbjct: 84  LALKLVETRLMQTIYIASDSQSVIKALKGKTRINTDWGIVIDDCILASKRFYNLSFNFSP 143

Query: 127 RSSNALADCVAKF 140
           R  N +A C+A +
Sbjct: 144 RKCNTVAHCLANW 156

[Close]

BLAST of Bhi12G000115 vs. ExPASy TrEMBL
Match: A0A803QN76 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 2.1e-10
Identity = 49/135 (36.30%), Postives = 67/135 (49.63%), Query Frame = 0


Query: 3    VPKVWLPPPPNRFKINTDAAWTFDFSSIGLGITERSFDGSIIAISTIHTQNIPNVSLAEP 62
            V K W PPPP+   +NTDA+   D    GL    R  DG++I   T+    I +V LAE 
Sbjct: 1278 VKKKWQPPPPDYVTLNTDASIIQDKMGCGLSAVIREHDGNLIVAKTLFLPGILSVHLAED 1337

Query: 63   LANKEGPKLAITLKCQNVILESDCMSVINLLQGKSSSIVEIECCIDDICSLQSSFTSSLF 122
             A + G  LA       VI  +D M V+  L+  S  I +    + +I SLQ  F    F
Sbjct: 1338 AAIRLGVLLAQRWSLPKVIASADSMGVVAALKSISPPISDWGVVVREIISLQQHFLFLQF 1397

Query: 123  SFAPRSSNALADCVA 138
            S+ PR  NA+A+ +A
Sbjct: 1398 SYVPRDCNAVANALA 1412

[Close]

BLAST of Bhi12G000115 vs. ExPASy TrEMBL
Match: A0A7J6HP67 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_004783 PE=4 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 2.1e-10
Identity = 49/135 (36.30%), Postives = 67/135 (49.63%), Query Frame = 0


Query: 3   VPKVWLPPPPNRFKINTDAAWTFDFSSIGLGITERSFDGSIIAISTIHTQNIPNVSLAEP 62
           V K W PPPP+   +NTDA+   D    GL    R  DG++I   T+    I +V LAE 
Sbjct: 522 VKKKWQPPPPDYVTLNTDASIIQDKMGCGLSAVIREHDGNLIVAKTLFLPGILSVHLAED 581

Query: 63  LANKEGPKLAITLKCQNVILESDCMSVINLLQGKSSSIVEIECCIDDICSLQSSFTSSLF 122
            A + G  LA       VI  +D M V+  L+  S  I +    + +I SLQ  F    F
Sbjct: 582 AAIRLGVLLAQRWSLPKVIASADSMGVVAALKSISPPISDWGVVVREIISLQQHFLFLQF 641

Query: 123 SFAPRSSNALADCVA 138
           S+ PR  NA+A+ +A
Sbjct: 642 SYVPRDCNAVANALA 656

The following BLAST results are available for this feature:

BLAST of Bhi12G000115 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G34320.1	1.6e-07	27.54	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT4G29090.1	2.1e-07	30.99	Ribonuclease H-like superfamily protein	[more]
AT4G09490.1	2.0e-05	30.37	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

BLAST of Bhi12G000115 vs. TAIR 10


Match Name	E-value	Identity	Description

BLAST of Bhi12G000115 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_024033146.1	1.7e-11	37.88	uncharacterized protein LOC112095446 [Citrus clementina]	[more]
XP_006491472.1	2.2e-11	35.04	uncharacterized protein LOC102626455 [Citrus sinensis]	[more]
XP_024046732.1	5.0e-11	37.12	uncharacterized protein LOC112101057 [Citrus clementina]	[more]
XP_015385025.1	5.0e-11	37.88	uncharacterized protein LOC107176703 [Citrus sinensis]	[more]
KAF7814428.1	8.5e-11	33.83	cysteine desulfurase mitochondrial-like [Senna tora]	[more]

BLAST of Bhi12G000115 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A803P4U9	1.2e-10	35.34	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]
A0A7J6GGV5	1.2e-10	35.34	Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_001227 PE=4 SV=1	[more]
A0A7J6FIX9	1.6e-10	36.09	RNase H domain-containing protein OS=Cannabis sativa OX=3483 GN=G4B88_026879 PE=...	[more]
A0A803QN76	2.1e-10	36.30	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]
A0A7J6HP67	2.1e-10	36.30	Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_004783 PE=4 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 13..140 e-value: 5.8E-9 score: 38.1
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 18..139 e-value: 5.3E-16 score: 58.6
None	No IPR available	PANTHER	PTHR33033	POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATED	coord: 6..138
None	No IPR available	PANTHER	PTHR33033:SF67	SUBFAMILY NOT NAMED	coord: 6..138
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 17..138 e-value: 1.82866E-14 score: 63.4872
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 14..139

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi12M000115	Bhi12M000115	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity