Bhi12G000082 (gene) Wax gourd (B227) v1

Overview
NameBhi12G000082
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionAB hydrolase-1 domain-containing protein
Locationchr12: 3030635 .. 3036442 (-)
RNA-Seq ExpressionBhi12G000082
SyntenyBhi12G000082
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAGCAAAAAACTCCCACCAATATCAACAACGGTAACACCTCTGACCGGAACTTCGCCACCGCACCGGCGGGACCCTCCGATCAATCTCTTTCAAAATCAAGATCATGGCGTGTTCAGGACCCATAATAATCACAGCCATTCCTCATCCATCTCCAAATTCCGATTCCTTCTCATTTCCCACCAATGGCGAGAGCCTTCTTGGGCTCCACGCATTCCTCTTCCCCACTAATCGCTTTACGCTTCTCTTCCTTTACCCCATCATTCATAACCAATCAACGACGCCGCTCCCTTCGTCCCACCCCCGCCACCGTCCTCGCTATGGCGGAGCTTCCGTCGGAGAAGCGCATTCAGCCCCACCCTTCGCTGGAGGTCCTCGGCGGAGGCTGCGATCGTTTCCTCCCAGCTTTTAAGGACTTGGATCTTCCCTATAAGCCTTTCCCTGTAATTGGGTCGAATCGCCATTTAGAGACAATTTTTGCTTCCTTCTTTAGGACTTGCCCCAATGTCAAGCTCCGCCGGGAGTGTCTCCGTGCAGCGGATAACGGTACCGTTGCTCTCGACTGGGTCGCCGGTGACGATCTCCGTTTGCCTCTTGATTCTCCTGTTCTTATTCTTCTGGTTTGTGCTTACTGATTCTTGGTTTCCTGAATTATTTATGAATTAGATAGGATGCCAAAGATATTCCAATTCCAAATGATATGAATCTTAGTGAATTGGAATTACGAACTCTATAGAAATTCCAATTCACGATTCTATTTCACATAGATTGAAAGAACGTGATGTATTTCATTCGAACGATGATGGCATACCAACAAATACATGGATAGTTGCCCAATTCGTTAATCCATTTAATCCTAATCTCTGTAGCCTTGGCTCTTTATTAAATTTTGAACTTGAGCTTGTAGTTTTATCCTTGAACGGGGTTGAGTTTTTGAAGCTTTAGTGTGGTTGGTCTTGTGAGGTTTAATCGTCTTAAGATGGGTGTTTGTGAATAGATCATCTTGTAGTTAGCATATTGGAGTTCGTTCTTGTAGTTTGTTTGCCCAAGTAAATTCTTTCATTTTTCTCAACAAAAGCTTGAGTTCGTACCAAAAGAACTATTTTATGGTTGGTTAAATGCATCGTTATTATATAGAGAATTTGGGAGTGTGGAGGTTAGGGATGGTTTATGGAAAACCAGTGTAAGTAGTGGATTGTGGGAGAGAGATATTTCTCTTGGAAGGATATTAATACTGTAATTTTATCTTTGATCTTGCAATATAAATTTACTGTTGTTTTCTTGTAATTGGGATCATAACATTCCCTTGATTCATATCAAAAGATTTCACATTGGTATCTTGAACTTCTGCAGCCTGGTTTAACAGGAGGGAGTCAAGATTCATATGTAAGACACATGTTGGTTAGAGCCAGAGATCGTGGGTGGAGGGTTGTGGTTTTCAACAGCCGCGGATGTGGGAATAGCCCTGTTACCACTCCCCAGGTATTCATTTTTTGTTCTTCTTTATGTTGGATTGGTGTATCTTATTGCTTTTTACATAAGATGTTTTTTCTTCCTTTTAGATATTCAAGAGTCGCTATTCTTGTTTATGATTCATTTTACAACACTGATATATGCAGTTCTATTCGGCTTCATTTTTAGGAGATATGCGTGAAGTAGTTGCGCTTGTTACTCAAAGATATCCCAAGGCTAATGTATATGCTGTTGGCTGGTCTCTTGGTGGTAACATTCTTGTTAATTATTTGGGACAGGTAAAAGTAAAATGATACATATTAGTCTCCATCGTTCTCTATACTCTACTTATTGGTTGCCATTTTGTTTAAACCTCCTAAAGTCTTATGGAATGAAGGAAATCGGAGTCTTGAATTTGTGAGGAAAAGCCATTTAGAATCATGTTTGTCATGATGACACAATTAAACAAAAAATAACTTTGTGGGCTTATGTTTGAAGTGGACAATATTTTATCATTTTAGAGGATCCGTTGTAACAATGGAAGAAGTTGGGCATATGTGAAATTGTGCCTAACTTCACCCATTTGTTTTCAATTTTGAAACATAAAACATATCAACTATGATATTAGTGTTGCTGCATTTCAAGATTTGTTTGTAATAATACCACTTGAAACAAATAAGCCTATTCTTTTGAAGATTACATTGCTGAATAACATATTTGGCGATAACGCAAAGTCGAATGCCTAAGAGTTCGGTGATCTGGGGACTCTCTTGTTTCTTTTAAACAAATGGGTTATGGAAATTTAAACTTCTGACCTCAAGAGAGTTAATACATGCTAATTATTGTTAAACTATGCTCACATGAGTTCTTATTAACACTGCCAGGGACAAGAACAAACTCTCTCTGTACAATGTTTAAGTTGCTTTTCCATATCATACTTATTCCAATTTCCATCTCTACGTTTTAAAAATCACTCCTACATCTTCATTGCAGGATTCCCTGTCCTGTCCATTGTCTGGTGCTGTGTCTTTGTGTAATCCATTCGATTTAATAGTTGCAGATGAGGACTTTCGCAAGGGCTTCAACAATGTTTATGACAAGGCCCTCGCTCGAGCTTTACATAAAATTTTCAACAAGTAATTCTCTTATCTAAATACGTTTTGTTTTGTTAGCTGTGCCATAATCTGAAAATGAAAAATTTGTATAGCCTTAGATTGTATCACCTTACTAGATTCTCCTGAGATTTCAGGCATGCTGATCTCTTCAATGATATGCCTGGTGAATTCAATATTCGCTTGGCTGCCAATGCTAAAACTGTTAGAGACTATGATAATGCCTTAACTCGCGGTATTCATTCTCTTCATTTTCGTAGTTTCGTTTGAAGGACATTGTCGATATGCACCATTTCTTCTGTTTAGAGCTTTTCTTTTTATTTCACTTTTTCCTTTTCCTTGAACAATTTTCAGTTTCATTTGGTTTCAACTCCGTGGATGAATACTACTCAAAGTCTAGCAGTTCAAAATTCATAAAAGACGTCAAAATTCCATTGCTTTGTATCCAGGTACCATTGTTTTCTTTTTTCTAATGTTGTCTTGTCTCATTGTTCTCCTAGAACTCAAGATTGCGGTTAAAAATCTCTAAGTTATTATCTATAAGGAAATAATACTTGTAAATACTATTTTGCAAACAGTTTCCAAACAACTCTTAAACTATCTTTTCCATTATATTGTTGTTCTTGTTAATGAGGAAGAGGATGATTTCTTCAAATACACAGTTTAACTTGCAGAATGTCTTGGAAAATTGTAGCTAGCTAGGAACATTGTTCTCCCACAGATTTGGGGCATTCCGTAGAATATTTTTCTTTTAATGTTGAGCAAGATCTGTAGCTAAAAGGTGGCATTTAATTTGCTTTTCAGGCAGCAAATGATCCAATTGCTCCAGACAGAGGAATTCCCCGCGAAGATATAAAGGTTCACTCTTGCTTATCTTTAAGTTCTTCAAGTATCTGTTAAAAATCTATTGAAAAGTTTAGTGCCATTTCATAAGATTTGATTTTTGGTATTTGAAAATTAGGCTTATAAAGACTACTTCCATCCATTGGTGCCATTATTTTGTTTTGTACTTTTTAGGAATGTTTCAAAACCTAAGCCAAATTTTGAAAGCTAAGGTATGGTTGATACCCCTTTTCGGTTTTTGGTTTTTGAAAATTAAACTTATAAACACTACTTCGATCTATTGGTTTCTTTGTTTTGTTATCTACTTCTTGGGATTGTGTCATAACCTTAGCCAAGTTTTGGAAGTGTTTCAAAACCTAAGCTAAGTTTTCAAAGCTAAGACTCATTATATAACCATTTGGTTTTTGGCTTTTGAAAATTAAGCTTATAAACACTGTCCATGTATAAGTTTCTTTGCTTTGTCATTCACTTTCTTAAAATGTTTTGAAAAACCAAACCAAGTTTTGAAAACTAAAAAAATAATTTATGCTCTGTTTGACAGTCATTTTGTTTTTAAGGTTTTATTGTTTGGTTTCACACAATTTCTTACAATAACTTTTATCTTTCCTAAAAAAACCATTTTAATTCTTAGCCAAATTTTAAAAACAAAAACAAGTTTTGAAAATCCTTTTCTTTCTTTGTTTTTCAAAAACTTTGCTCTAATTTTGAAAACAGTGTTAAAAAGTGAATGGAATGACATAAAAATCTAAATGTGGAATAAGTTTTTATAAGCTTAATTTTAAAAAACCAAAGGTCAAACTATTATCAAGTAAGTGTAGTTTTTAAAGAAAGATAAAAACCATAGTTAGGAAATTGAGAGGAGACAAAGCATAAATTTCAAAAACCTAAAACAAATAACAAATTGAGGCCTAAAAGAGTAGTTTTTATCTTTTAAATATTGTCAAGAATTCAAATATTTACACAAGAAGGAAAGATAAAAATATAAACAAGTTTCTAGAGAACAAACACAATTTTTAAGAATCTAGAAATAAAATGCTAAATAGTCATCGAACAAGACCTTAAATATAGTCTATCATCTAAGTTTCCAGAGTTTGTGAAAGTTTAACCTATTATCAAAGTAGGAGATTCTATATTCGCATCTGCATCCTTCTTGTGATTAGTACACATATTTTTCAAATGTGACTGTTGTTGCAGGAAAATCCGAACTGTTTGTTAATAGTAACACCGAAAGGTGGGCATCTAGGGTGGGTTGCAGGCCCCGGCGCTCCCCTCGGTTTTCCTTGGACCGATCCTTTCGTGATTGATTTCCTCGTGTATCTGGAAAACCACACAGCGAAAGCCTCTGCATCTTCTATAGGTTTAACACCTGCAGAGGTAATGACGTAGCGATTGATAGTGACATCTATGAGCACAGGTAGAAACATTGAAAATAGTGGTTAGCCATCCTCAGTAGTTGATTTGTGAGATGATTAAACATAATGCCATGTATAGATTAGTTAAAAGTATAAAATGTAGGTTGAGTATTCTTTGTTTTCCTTTCAAAAGGAGACAGAGAAATGGCATTCTTTATTTGTGCATTTCTCCAGCCACCCTTTTAGCTAGTGTTTATCTTGGTAATATGTTTTGTAATGTTCAGAGATCTTTATTTTTTTTATTGTCTTTGCTTGGCTTCCATACAAGATGTGAATGTGTTTTTTTTTCCATGAATGTTTTAATCTTGGTTGACTGATTGAAAATTATGAACTAGATGTAATACTGTTTCTTTATATTTGGTTAGAAAGAAATAATGATTGGGGATGCTTCTTGATTTTTCTCATGTCTCTTTCTTTCCACAAGTCACAAGCTATGATTAGGACTTGGGACAAATTGCATAAACAGAACTTTCACCAAAATAATGTGAAAGTGTAATTCATTTTCTGCAATTAATTTTGTATACGTAGTGGGTATTTATAGAAGATAACCCATAAGTTACCGAGAGATTACAAAGGCTTAACAAATAACAAAATAAATCTTAATAACAAAACAAATTTTAACAAAATAACAGACTATATTAGTTAATATCCTCCCGCAAATTGATGATAGATCATCCACAAGCCATCAGTTTGGACCTCAACAAATAGAAACGACCTGTTGTAAGAGCCTTCGTTAATATGTCAACAAGCTTATGAAGTGAAAATCGACTTCAATATACTTCGTACGACTGTGGTACAAAGTATTGCAGGCTAACTGAGTTGTTGATTTATTGTCACACCAGATTGTTGGAGGAGAAGATCCAAATATATGAAGATCACACAATATTTGACGAATCCAAAATAAATCTGGAGCAGTGGTGGCTAATGCCCTATATTCAACTTCAGTAGAACTCCTAAAGACAATAGTTTGCTTCTTTGCTGCCCAAG

mRNA sequence

AAAAAAGCAAAAAACTCCCACCAATATCAACAACGGTAACACCTCTGACCGGAACTTCGCCACCGCACCGGCGGGACCCTCCGATCAATCTCTTTCAAAATCAAGATCATGGCGTGTTCAGGACCCATAATAATCACAGCCATTCCTCATCCATCTCCAAATTCCGATTCCTTCTCATTTCCCACCAATGGCGAGAGCCTTCTTGGGCTCCACGCATTCCTCTTCCCCACTAATCGCTTTACGCTTCTCTTCCTTTACCCCATCATTCATAACCAATCAACGACGCCGCTCCCTTCGTCCCACCCCCGCCACCGTCCTCGCTATGGCGGAGCTTCCGTCGGAGAAGCGCATTCAGCCCCACCCTTCGCTGGAGGTCCTCGGCGGAGGCTGCGATCGTTTCCTCCCAGCTTTTAAGGACTTGGATCTTCCCTATAAGCCTTTCCCTGTAATTGGGTCGAATCGCCATTTAGAGACAATTTTTGCTTCCTTCTTTAGGACTTGCCCCAATGTCAAGCTCCGCCGGGAGTGTCTCCGTGCAGCGGATAACGGTACCGTTGCTCTCGACTGGGTCGCCGGTGACGATCTCCGTTTGCCTCTTGATTCTCCTGTTCTTATTCTTCTGCCTGGTTTAACAGGAGGGAGTCAAGATTCATATGTAAGACACATGTTGGTTAGAGCCAGAGATCGTGGGTGGAGGGTTGTGGTTTTCAACAGCCGCGGATGTGGGAATAGCCCTGTTACCACTCCCCAGTTCTATTCGGCTTCATTTTTAGGAGATATGCGTGAAGTAGTTGCGCTTGTTACTCAAAGATATCCCAAGGCTAATGTATATGCTGTTGGCTGGTCTCTTGGTGGTAACATTCTTGTTAATTATTTGGGACAGGATTCCCTGTCCTGTCCATTGTCTGGTGCTGTGTCTTTGTGTAATCCATTCGATTTAATAGTTGCAGATGAGGACTTTCGCAAGGGCTTCAACAATGTTTATGACAAGGCCCTCGCTCGAGCTTTACATAAAATTTTCAACAAGCATGCTGATCTCTTCAATGATATGCCTGGTGAATTCAATATTCGCTTGGCTGCCAATGCTAAAACTGTTAGAGACTATGATAATGCCTTAACTCGCGTTTCATTTGGTTTCAACTCCGTGGATGAATACTACTCAAAGTCTAGCAGTTCAAAATTCATAAAAGACGTCAAAATTCCATTGCTTTGTATCCAGGCAGCAAATGATCCAATTGCTCCAGACAGAGGAATTCCCCGCGAAGATATAAAGGAAAATCCGAACTGTTTGTTAATAGTAACACCGAAAGGTGGGCATCTAGGGTGGGTTGCAGGCCCCGGCGCTCCCCTCGGTTTTCCTTGGACCGATCCTTTCGTGATTGATTTCCTCGTGTATCTGGAAAACCACACAGCGAAAGCCTCTGCATCTTCTATAGGTTTAACACCTGCAGAGATTGTTGGAGGAGAAGATCCAAATATATGAAGATCACACAATATTTGACGAATCCAAAATAAATCTGGAGCAGTGGTGGCTAATGCCCTATATTCAACTTCAGTAGAACTCCTAAAGACAATAGTTTGCTTCTTTGCTGCCCAAG

Coding sequence (CDS)

ATGGCGAGAGCCTTCTTGGGCTCCACGCATTCCTCTTCCCCACTAATCGCTTTACGCTTCTCTTCCTTTACCCCATCATTCATAACCAATCAACGACGCCGCTCCCTTCGTCCCACCCCCGCCACCGTCCTCGCTATGGCGGAGCTTCCGTCGGAGAAGCGCATTCAGCCCCACCCTTCGCTGGAGGTCCTCGGCGGAGGCTGCGATCGTTTCCTCCCAGCTTTTAAGGACTTGGATCTTCCCTATAAGCCTTTCCCTGTAATTGGGTCGAATCGCCATTTAGAGACAATTTTTGCTTCCTTCTTTAGGACTTGCCCCAATGTCAAGCTCCGCCGGGAGTGTCTCCGTGCAGCGGATAACGGTACCGTTGCTCTCGACTGGGTCGCCGGTGACGATCTCCGTTTGCCTCTTGATTCTCCTGTTCTTATTCTTCTGCCTGGTTTAACAGGAGGGAGTCAAGATTCATATGTAAGACACATGTTGGTTAGAGCCAGAGATCGTGGGTGGAGGGTTGTGGTTTTCAACAGCCGCGGATGTGGGAATAGCCCTGTTACCACTCCCCAGTTCTATTCGGCTTCATTTTTAGGAGATATGCGTGAAGTAGTTGCGCTTGTTACTCAAAGATATCCCAAGGCTAATGTATATGCTGTTGGCTGGTCTCTTGGTGGTAACATTCTTGTTAATTATTTGGGACAGGATTCCCTGTCCTGTCCATTGTCTGGTGCTGTGTCTTTGTGTAATCCATTCGATTTAATAGTTGCAGATGAGGACTTTCGCAAGGGCTTCAACAATGTTTATGACAAGGCCCTCGCTCGAGCTTTACATAAAATTTTCAACAAGCATGCTGATCTCTTCAATGATATGCCTGGTGAATTCAATATTCGCTTGGCTGCCAATGCTAAAACTGTTAGAGACTATGATAATGCCTTAACTCGCGTTTCATTTGGTTTCAACTCCGTGGATGAATACTACTCAAAGTCTAGCAGTTCAAAATTCATAAAAGACGTCAAAATTCCATTGCTTTGTATCCAGGCAGCAAATGATCCAATTGCTCCAGACAGAGGAATTCCCCGCGAAGATATAAAGGAAAATCCGAACTGTTTGTTAATAGTAACACCGAAAGGTGGGCATCTAGGGTGGGTTGCAGGCCCCGGCGCTCCCCTCGGTTTTCCTTGGACCGATCCTTTCGTGATTGATTTCCTCGTGTATCTGGAAAACCACACAGCGAAAGCCTCTGCATCTTCTATAGGTTTAACACCTGCAGAGATTGTTGGAGGAGAAGATCCAAATATATGA

Protein sequence

MARAFLGSTHSSSPLIALRFSSFTPSFITNQRRRSLRPTPATVLAMAELPSEKRIQPHPSLEVLGGGCDRFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPNVKLRRECLRAADNGTVALDWVAGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVALVTQRYPKANVYAVGWSLGGNILVNYLGQDSLSCPLSGAVSLCNPFDLIVADEDFRKGFNNVYDKALARALHKIFNKHADLFNDMPGEFNIRLAANAKTVRDYDNALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPDRGIPREDIKENPNCLLIVTPKGGHLGWVAGPGAPLGFPWTDPFVIDFLVYLENHTAKASASSIGLTPAEIVGGEDPNI
Homology
BLAST of Bhi12G000082 vs. TAIR 10
Match: AT3G50790.1 (esterase/lipase/thioesterase family protein )

HSP 1 Score: 561.2 bits (1445), Expect = 7.1e-160
Identity = 272/388 (70.10%), Postives = 322/388 (82.99%), Query Frame = 0

Query: 20  FSSFTPSFITNQRRRSLRPTPATVLAMAELPSEKRIQPHPSLEVLGGGCDRFLPAFKD-L 79
           F++ +P  I++   R+LR    +V A A  P   R   H SLEV+GGG DRFLP+ KD L
Sbjct: 6   FATSSPPLISSV--RTLR-RHFSVFAAAN-PEMSRPSHHSSLEVIGGGSDRFLPSLKDSL 65

Query: 80  DLPYKPFPVIGSNRHLETIFASFFRTCPNVKLRRECLRAADNGTVALDWVAGDDLRLPLD 139
             PY  FP+IG NRH+ETI+ASFFR+ P V+LRRECLR  DNG+VALDWVAG+D   P D
Sbjct: 66  AKPYNAFPLIGFNRHVETIYASFFRSVPFVRLRRECLRTKDNGSVALDWVAGEDRHFPPD 125

Query: 140 SPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDM 199
           SP+LILLPGLTGGSQDSYVRHML+RA+ + WR VVFNSRGCG+SPVTTPQFYSASFLGD+
Sbjct: 126 SPILILLPGLTGGSQDSYVRHMLLRAQSKKWRCVVFNSRGCGDSPVTTPQFYSASFLGDI 185

Query: 200 REVVALVTQRYPKANVYAVGWSLGGNILVNYLGQDSLSCPLSGAVSLCNPFDLIVADEDF 259
            EV+  V  ++PKAN+YA GWSLGGNILVNYLGQ+S +CPL+ AVSLCNPFDL++ADEDF
Sbjct: 186 GEVIDHVVDKFPKANLYAAGWSLGGNILVNYLGQESHNCPLTAAVSLCNPFDLVIADEDF 245

Query: 260 RKGFNNVYDKALARALHKIFNKHADLFNDMPGEFNIRLAANAKTVRDYDNALTRVSFGFN 319
            KGFNNVYDKAL+++L +IF+KH+ LF D+ GEFNI LAANA+TVRD+D+ LTRVSFGF 
Sbjct: 246 HKGFNNVYDKALSKSLRRIFSKHSLLFEDIGGEFNIPLAANAETVRDFDDGLTRVSFGFK 305

Query: 320 SVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPDRGIPREDIKENPNCLLIVTPKGGHL 379
           SVDEYYSKSSSSK IK V+IPLLCIQAANDPIAP+RGIPR+DIK NPNC+LIVTP+GGHL
Sbjct: 306 SVDEYYSKSSSSKAIKHVRIPLLCIQAANDPIAPERGIPRDDIKANPNCVLIVTPRGGHL 365

Query: 380 GWVAGPGAPLGFPWTDPFVIDFLVYLEN 407
           GWVAG  AP G PWTD  V++FL ++EN
Sbjct: 366 GWVAGEEAPNGAPWTDQVVMEFLQHVEN 389

BLAST of Bhi12G000082 vs. TAIR 10
Match: AT5G49950.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 219.2 bits (557), Expect = 6.6e-57
Identity = 109/307 (35.50%), Postives = 175/307 (57.00%), Query Frame = 0

Query: 90  SNRHLETIFASFFRTCPNVKLRRECLRAADNGTVALDWVAGDDLRLPLD----------- 149
           S+ HL+T F + F   P    +R   +A D GT+ALDW+   D+   +            
Sbjct: 87  SSPHLQTAFLTLFGKSPPFSYKRILYQATDGGTIALDWLMHSDVVEGISQVVNASNPGTD 146

Query: 150 -SPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGD 209
            +P+ I++PGLT  S  +Y++H+  R    GW VVV N RG G   +T+   Y+A +  D
Sbjct: 147 RTPIAIIVPGLTSDSSAAYIKHIAFRLAKEGWNVVVQNHRGLGGISLTSDCVYTAGWTED 206

Query: 210 MREVVALVTQRYPKANVYAVGWSLGGNILVNYLGQDSLSCPLSGAVSLCNPFDLIVADED 269
           +R+V+A +  ++P+A ++AVG S+G N+LV YLG+D  + PL GA ++C+P+DL++ D  
Sbjct: 207 LRKVIAHIHSQFPEAPLFAVGTSIGANVLVKYLGEDGPNTPLIGATAVCSPWDLLICDRF 266

Query: 270 F-RKGFNNVYDKALARALHKIFNKHADLFNDMPGEFNIRLAANAKTVRDYDNALTRVSFG 329
             RK    VYD+ L   L      H  + + +     I+    +++VR++DN  TR+   
Sbjct: 267 INRKLVQKVYDRMLTIGLQGYAQLHHSIISRIADWEGIK---KSRSVREFDNYATRLVAK 326

Query: 330 FNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPDRGIPREDIKENPNCLLIVTPKGG 384
           F + D YY +SSSS+++++V +PLLCI A +DP+     IP ++ + N N +L  T  GG
Sbjct: 327 FETTDTYYRRSSSSQYVENVAVPLLCISALDDPVCTREAIPWDECRANKNIVLATTTHGG 386

BLAST of Bhi12G000082 vs. TAIR 10
Match: AT1G34340.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 210.3 bits (534), Expect = 3.1e-54
Identity = 107/306 (34.97%), Postives = 169/306 (55.23%), Query Frame = 0

Query: 93  HLETIFASFFRTCPNVKLRRECLRAADNGTVALDWVAGDDLRLPLD-------------- 152
           H++T F +F    P     R+    +D GT+ALDW+   D+   LD              
Sbjct: 96  HIQTCFLNFHGLPPVFTYTRQLFLTSDGGTIALDWLTNSDV---LDGSLHNKSEITKEDT 155

Query: 153 SPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDM 212
           +P+ +++PGLT  S  +Y++H+       GW VV+ N RG G   VT+  FY+A +  D+
Sbjct: 156 TPIAVVIPGLTSDSSSAYLKHLAYDTAKTGWNVVISNHRGLGGVSVTSDCFYNAGWTDDI 215

Query: 213 REVVALVTQRYPKANVYAVGWSLGGNILVNYLGQDSLSCPLSGAVSLCNPFDLIVADEDF 272
           R V+  +  +YP+A ++A+G S+G N+LV YLG++    PL GAV++C+P+DL++ D   
Sbjct: 216 RVVLDYLQHKYPRAPLFAIGTSIGANVLVKYLGEEGEKTPLRGAVAICSPWDLLIGDRFI 275

Query: 273 -RKGFNNVYDKALARALHKIFNKHADLFNDMPGEFNIRLAANAKTVRDYDNALTRVSFGF 332
            R     +YDKAL   L      H   F  +     I+    ++++RD+DN  T +   F
Sbjct: 276 CRTLKQKLYDKALTIGLQGYAQLHEPQFLRLANWEGIK---KSRSIRDFDNHATCLVGKF 335

Query: 333 NSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPDRGIPREDIKENPNCLLIVTPKGGH 384
            +VD YY KSSS++++ +V +PLLCI A +DP+     IP ++ + N N +L  T  GGH
Sbjct: 336 ETVDTYYRKSSSTQYVGNVAVPLLCISALDDPLCTKEAIPWDECRANKNIVLATTNHGGH 395

BLAST of Bhi12G000082 vs. TAIR 10
Match: AT2G03140.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 166.0 bits (419), Expect = 6.6e-41
Identity = 100/301 (33.22%), Postives = 158/301 (52.49%), Query Frame = 0

Query: 108 VKLRRECLRAADNGTVALDWVAGDDLR--LPLDSPVLILLPGLTGGSQDSYVRHMLVRAR 167
           ++ +R C+   D G V+LDW A  D+R    LD+ V + +PG   GS +  VR  +  A 
Sbjct: 168 LEYQRVCITMEDGGVVSLDWPANLDIREERGLDTTV-VFIPGTPEGSMEEGVRSFVCEAL 227

Query: 168 DRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVALVTQRYPKANVYAVGWSLGGNI 227
            RG   VV N RGC  SP+TTP+ ++A    D+   +  +++  P   + AVG   G N+
Sbjct: 228 RRGVFPVVMNPRGCAGSPLTTPRLFTAGDSDDISTALRFLSKTRPWTTLTAVGRGYGANM 287

Query: 228 LVNYLGQDSLSCPLSGAVSLCNPFDLIVADEDFRKG-FNNVYDKALARALHKIFNKHADL 287
           L  YL +     PL+ AV + NPFDL   +E  R   ++   D+ L R L +I   + +L
Sbjct: 288 LTKYLAEAGERTPLTAAVCIDNPFDL---EEITRTSPYSTSLDQQLTRGLVEILLANKEL 347

Query: 288 FNDMPGEFNIRLAANAKTVRDYDNALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQ 347
           F      F++  A  +K+VR++D AL+ V++G  S++++YS  ++ + I +VK+PLL IQ
Sbjct: 348 FQGRAKAFDVGKALCSKSVREFDKALSMVTYGCESIEDFYSSCATREVIGEVKVPLLFIQ 407

Query: 348 AANDPIAPDRGIPREDIKENPNCLLIVTPKGGHLGWVAGPGAPLGFPWTDPFVIDFLVYL 406
             ND + P   IPR  I ENP   L++     +L      G  +   W      ++L  +
Sbjct: 408 --NDDVVPPYTIPRSSIAENPFTSLLLCSSSPNL----IDGRTVAVSWCQDLASEWLTAV 458

BLAST of Bhi12G000082 vs. TAIR 10
Match: AT2G03140.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 166.0 bits (419), Expect = 6.6e-41
Identity = 100/301 (33.22%), Postives = 158/301 (52.49%), Query Frame = 0

Query: 108 VKLRRECLRAADNGTVALDWVAGDDLR--LPLDSPVLILLPGLTGGSQDSYVRHMLVRAR 167
           ++ +R C+   D G V+LDW A  D+R    LD+ V + +PG   GS +  VR  +  A 
Sbjct: 168 LEYQRVCITMEDGGVVSLDWPANLDIREERGLDTTV-VFIPGTPEGSMEEGVRSFVCEAL 227

Query: 168 DRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVALVTQRYPKANVYAVGWSLGGNI 227
            RG   VV N RGC  SP+TTP+ ++A    D+   +  +++  P   + AVG   G N+
Sbjct: 228 RRGVFPVVMNPRGCAGSPLTTPRLFTAGDSDDISTALRFLSKTRPWTTLTAVGRGYGANM 287

Query: 228 LVNYLGQDSLSCPLSGAVSLCNPFDLIVADEDFRKG-FNNVYDKALARALHKIFNKHADL 287
           L  YL +     PL+ AV + NPFDL   +E  R   ++   D+ L R L +I   + +L
Sbjct: 288 LTKYLAEAGERTPLTAAVCIDNPFDL---EEITRTSPYSTSLDQQLTRGLVEILLANKEL 347

Query: 288 FNDMPGEFNIRLAANAKTVRDYDNALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQ 347
           F      F++  A  +K+VR++D AL+ V++G  S++++YS  ++ + I +VK+PLL IQ
Sbjct: 348 FQGRAKAFDVGKALCSKSVREFDKALSMVTYGCESIEDFYSSCATREVIGEVKVPLLFIQ 407

Query: 348 AANDPIAPDRGIPREDIKENPNCLLIVTPKGGHLGWVAGPGAPLGFPWTDPFVIDFLVYL 406
             ND + P   IPR  I ENP   L++     +L      G  +   W      ++L  +
Sbjct: 408 --NDDVVPPYTIPRSSIAENPFTSLLLCSSSPNL----IDGRTVAVSWCQDLASEWLTAV 458

BLAST of Bhi12G000082 vs. ExPASy Swiss-Prot
Match: Q40863 (Embryogenesis-associated protein EMB8 OS=Picea glauca OX=3330 GN=EMB8 PE=2 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 4.8e-129
Identity = 228/350 (65.14%), Postives = 267/350 (76.29%), Query Frame = 0

Query: 67  GCDR-FLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPNVKLRRECLRAADNGTVAL 126
           G DR  +     L  PY+ FP +G NRH+ETIFASFFR+ P +K RRECLR  D GTV L
Sbjct: 72  GSDRELMSKLTTLGRPYRHFPFMG-NRHVETIFASFFRSWPVIKSRRECLRMEDGGTVEL 131

Query: 127 DW-VAGDDL-----RLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGC 186
           DW + G+D       LP++SPVLILLPGLTGGS DSYV+HML+RAR  GW  VVFNSRGC
Sbjct: 132 DWPLEGEDAELWNGELPVNSPVLILLPGLTGGSDDSYVKHMLLRARKHGWHSVVFNSRGC 191

Query: 187 GNSPVTTPQFYSASFLGDMREVVALVTQRYPKANVYAVGWSLGGNILVNYLGQDSLSCPL 246
            +SPVTTPQFYSASF  D+ +VV  V  R+ ++N+YAVGWSLG NILV YLG+ + +CPL
Sbjct: 192 ADSPVTTPQFYSASFTKDLCQVVKHVAVRFSESNIYAVGWSLGANILVRYLGEVAGNCPL 251

Query: 247 SGAVSLCNPFDLIVADEDFRK--GFNNVYDKALARALHKIFNKHADLFNDMPGEFNIRLA 306
           SGAVSLCNPF+L++ADEDF K  GFNNVYDKALAR L +IF KH  LF  + GE+NI   
Sbjct: 252 SGAVSLCNPFNLVIADEDFHKGLGFNNVYDKALARGLRQIFPKHTRLFEGIEGEYNIPTV 311

Query: 307 ANAKTVRDYDNALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPDRGIP 366
           A A++VRD+D  LTRVSFGF SV +YYS SSSS  IK V+  LLCIQA+NDPIAP RGIP
Sbjct: 312 AKARSVRDFDGGLTRVSFGFQSVGDYYSNSSSSLSIKYVQTSLLCIQASNDPIAPSRGIP 371

Query: 367 REDIKENPNCLLIVTPKGGHLGWVAGPGAPLGFPWTDPFVIDFLVYLENH 408
            EDIKENPNCLL+VTP GGHLGWVAG  AP G PWTDP V+++L  LE +
Sbjct: 372 WEDIKENPNCLLVVTPNGGHLGWVAGDDAPFGAPWTDPLVMEYLEVLEKN 420

BLAST of Bhi12G000082 vs. ExPASy Swiss-Prot
Match: Q0VC00 (Phospholipase ABHD3 OS=Bos taurus OX=9913 GN=ABHD3 PE=2 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 3.1e-51
Identity = 101/293 (34.47%), Postives = 165/293 (56.31%), Query Frame = 0

Query: 95  ETIFASFFRTCPNVKLRRECLRAADNGTVALDWVAGDDLRLPLDS---PVLILLPGLTGG 154
           +T+   F  + P V+ R E ++ AD G ++LDW   D+ +  +D+   P ++LLPGLTG 
Sbjct: 92  QTLLRPFITSKPLVQYRNELIKTADGGQISLDWFDNDNSKHYMDASTRPTVLLLPGLTGT 151

Query: 155 SQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVALVTQRYPK 214
           S++SY+ HM+  + + G+R VVFN+RG     + TP+ Y  S   D+  V+  V   YP 
Sbjct: 152 SKESYILHMIHLSEELGYRYVVFNNRGVAGENLLTPRTYCCSNTEDLETVIHHVHSLYPS 211

Query: 215 ANVYAVGWSLGGNILVNYLGQDSLSCPLSGAVSLCNPFDLIVADEDFRKGFN-NVYDKAL 274
           A   A G S+GG +L+NYLG+     PL  A +    ++     E   K  N  +++  L
Sbjct: 212 APFLAAGVSMGGMLLLNYLGKIGPKTPLKAAATFSVGWNTFACSESLEKPLNWLLFNYYL 271

Query: 275 ARALHKIFNKHADLFNDMPGEFNIRLAANAKTVRDYDNALTRVSFGFNSVDEYYSKSSSS 334
              L    NKH  +F     + ++     AK++R++D   T V FG+ ++D+YY+ +S +
Sbjct: 272 TTCLQSSVNKHRHMF---VKQIDVDHVMKAKSIREFDKRFTSVMFGYRTIDDYYTDASPN 331

Query: 335 KFIKDVKIPLLCIQAANDPIAPDRGIPREDIKENPNCLLIVTPKGGHLGWVAG 384
           + +K V IP+LC+ + +D  +P   IP E  K+NPN  L++T  GGH+G++ G
Sbjct: 332 RRLKSVGIPVLCLNSVDDVFSPSHAIPIETAKQNPNVALVLTSYGGHIGFLEG 381

BLAST of Bhi12G000082 vs. ExPASy Swiss-Prot
Match: Q8WU67 (Phospholipase ABHD3 OS=Homo sapiens OX=9606 GN=ABHD3 PE=2 SV=2)

HSP 1 Score: 202.2 bits (513), Expect = 1.2e-50
Identity = 101/293 (34.47%), Postives = 163/293 (55.63%), Query Frame = 0

Query: 95  ETIFASFFRTCPNVKLRRECLRAADNGTVALDWVAGDDLRLPLDS---PVLILLPGLTGG 154
           +T+   F  + P V+ R E ++ AD G ++LDW   D+    +D+   P ++LLPGLTG 
Sbjct: 92  QTLLRPFITSKPPVQYRNELIKTADGGQISLDWFDNDNSTCYMDASTRPTILLLPGLTGT 151

Query: 155 SQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVALVTQRYPK 214
           S++SY+ HM+  + + G+R VVFN+RG     + TP+ Y  +   D+  V+  V   YP 
Sbjct: 152 SKESYILHMIHLSEELGYRCVVFNNRGVAGENLLTPRTYCCANTEDLETVIHHVHSLYPS 211

Query: 215 ANVYAVGWSLGGNILVNYLGQDSLSCPLSGAVSLCNPFDLIVADEDFRKGFN-NVYDKAL 274
           A   A G S+GG +L+NYLG+     PL  A +    ++     E   K  N  +++  L
Sbjct: 212 APFLAAGVSMGGMLLLNYLGKIGSKTPLMAAATFSVGWNTFACSESLEKPLNWLLFNYYL 271

Query: 275 ARALHKIFNKHADLFNDMPGEFNIRLAANAKTVRDYDNALTRVSFGFNSVDEYYSKSSSS 334
              L    NKH  +F     + ++     AK++R++D   T V FG+ ++D+YY+ +S S
Sbjct: 272 TTCLQSSVNKHRHMF---VKQVDMDHVMKAKSIREFDKRFTSVMFGYQTIDDYYTDASPS 331

Query: 335 KFIKDVKIPLLCIQAANDPIAPDRGIPREDIKENPNCLLIVTPKGGHLGWVAG 384
             +K V IP+LC+ + +D  +P   IP E  K+NPN  L++T  GGH+G++ G
Sbjct: 332 PRLKSVGIPVLCLNSVDDVFSPSHAIPIETAKQNPNVALVLTSYGGHIGFLEG 381

BLAST of Bhi12G000082 vs. ExPASy Swiss-Prot
Match: Q91ZH7 (Phospholipase ABHD3 OS=Mus musculus OX=10090 GN=Abhd3 PE=1 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 1.7e-49
Identity = 99/293 (33.79%), Postives = 164/293 (55.97%), Query Frame = 0

Query: 95  ETIFASFFRTCPNVKLRRECLRAADNGTVALDWVAGDDLRLPLDS---PVLILLPGLTGG 154
           +T+   F  + P V+ R E ++ AD G ++LDW   ++    +D+   P ++LLPGLTG 
Sbjct: 92  QTLLRPFITSKPPVQYRNELIKTADGGQISLDWFDNNNSAYYVDASTRPTILLLPGLTGT 151

Query: 155 SQDSYVRHMLVRARDRGWRVVVFNSRGCGNSPVTTPQFYSASFLGDMREVVALVTQRYPK 214
           S++SY+ HM+  + + G+R VVFN+RG     + TP+ Y  +   D+  VV  V   YP 
Sbjct: 152 SKESYILHMIHLSEELGYRCVVFNNRGVAGESLLTPRTYCCANTEDLEAVVHHVHSLYPG 211

Query: 215 ANVYAVGWSLGGNILVNYLGQDSLSCPLSGAVSLCNPFDLIVADEDFRKGFN-NVYDKAL 274
           A   A G S+GG +L+NYLG+     PL  A +    ++     E   +  N  +++  L
Sbjct: 212 APFLAAGVSMGGMLLLNYLGKIGSKTPLMAAATFSVGWNTFACSESLERPLNWLLFNYYL 271

Query: 275 ARALHKIFNKHADLFNDMPGEFNIRLAANAKTVRDYDNALTRVSFGFNSVDEYYSKSSSS 334
              L     KH  +F +   + ++     AK++R++D   T V FG+ ++D+YY+ +S +
Sbjct: 272 TTCLQSSVKKHRHMFVE---QIDMDQVMKAKSIREFDKRFTAVMFGYRTLDDYYTDASPN 331

Query: 335 KFIKDVKIPLLCIQAANDPIAPDRGIPREDIKENPNCLLIVTPKGGHLGWVAG 384
           + +K V IP+LC+ A +D  +P   IP E  K+NPN  L++T  GGH+G++ G
Sbjct: 332 RRLKSVGIPVLCLNATDDVFSPSHAIPIETAKQNPNVALVLTAYGGHIGFLEG 381

BLAST of Bhi12G000082 vs. ExPASy Swiss-Prot
Match: Q54H38 (Abhydrolase domain-containing protein OS=Dictyostelium discoideum OX=44689 GN=abhd PE=1 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 3.5e-47
Identity = 114/335 (34.03%), Postives = 182/335 (54.33%), Query Frame = 0

Query: 73  PAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPNVKLRRECL-RAADNGTVALDWVAGD 132
           P  K+    Y P+ +   N H    + S+     N+K RRE L    D GT++LD+    
Sbjct: 53  PTIKNGVTFYPPYYLY--NSHFMNYYGSYKIPKLNLKTRREILVNPIDGGTISLDFFELG 112

Query: 133 DLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRA-RDRGWRVVVFNSRGCGNSPVTTPQFY 192
           + +   D+P +++  GLTGGS + YV++   RA +++G+R VVFN RGC  +P+T  + Y
Sbjct: 113 EFK--EDTPTIVINHGLTGGSHERYVQYFAQRAYKEKGFRSVVFNYRGCAGNPITADRAY 172

Query: 193 SASFLGDMREVVALVTQ-RYPKANV-YAVGWSLGGNILVNYLGQDSLSCPLSGAVSLCNP 252
           SA  L D++ V   +T+   P     + VG+SLG  ILVNY+       P    VS+ NP
Sbjct: 173 SAVQLDDIKFVTEYLTKTALPLVKKWFLVGFSLGSAILVNYMADAGKDSPYLAHVSISNP 232

Query: 253 FDLIVADEDFRKGF--NNVYDKALARALHKIFNKHADLFNDMPGEFNIRLAANAKTVRDY 312
            +++   ++    +  N +Y+K LA  L ++F K     +    +  I     A+T+ D+
Sbjct: 233 MNMVECTKNLSSTYINNLIYNKGLANNLKRLFRKFDGRLDKYATKEQIMA---AQTIADF 292

Query: 313 DNALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPDRGIPREDIKENPN 372
           D+ +T   FGF +  +YY  +SSSK I+++  P+L I A +DPIAP  G P +D K NPN
Sbjct: 293 DDLITSKMFGFETAHDYYLAASSSKSIRNLVKPILFINAIDDPIAPTSGFPWKDFKSNPN 352

Query: 373 CLLIVTPKGGHLGWVAGPGAPLGFPWTDPFVIDFL 402
            +L V+  GGHLG+++         W+D   +++L
Sbjct: 353 TILCVSRWGGHLGFISYED---HMSWSDKAAVEYL 377

BLAST of Bhi12G000082 vs. NCBI nr
Match: XP_038875981.1 (embryogenesis-associated protein EMB8 [Benincasa hispida])

HSP 1 Score: 872.1 bits (2252), Expect = 2.0e-249
Identity = 422/424 (99.53%), Postives = 424/424 (100.00%), Query Frame = 0

Query: 1   MARAFLGSTHSSSPLIALRFSSFTPSFITNQRRRSLRPTPATVLAMAELPSEKRIQPHPS 60
           MARAFLGSTHSSSPLIALRFSSFTPSFITNQRRRSLRPTPATVLAMAELPSEKRIQPHPS
Sbjct: 1   MARAFLGSTHSSSPLIALRFSSFTPSFITNQRRRSLRPTPATVLAMAELPSEKRIQPHPS 60

Query: 61  LEVLGGGCDRFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPNVKLRRECLRAADN 120
           LEVLGGGCDRFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPNVKLRRECLRAADN
Sbjct: 61  LEVLGGGCDRFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPNVKLRRECLRAADN 120

Query: 121 GTVALDWVAGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCG 180
           GTVALDWVAGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCG
Sbjct: 121 GTVALDWVAGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCG 180

Query: 181 NSPVTTPQFYSASFLGDMREVVALVTQRYPKANVYAVGWSLGGNILVNYLGQDSLSCPLS 240
           NSPVTTPQFYSASFLGDMREVVALVTQRYPKANVYAVGWSLGGNILVNYLGQDSLSCPLS
Sbjct: 181 NSPVTTPQFYSASFLGDMREVVALVTQRYPKANVYAVGWSLGGNILVNYLGQDSLSCPLS 240

Query: 241 GAVSLCNPFDLIVADEDFRKGFNNVYDKALARALHKIFNKHADLFNDMPGEFNIRLAANA 300
           GAVSLCNPFDLIVADEDFRKGFNNVYDKALARALHKIFNKHADLFNDMPGEFNIRLAANA
Sbjct: 241 GAVSLCNPFDLIVADEDFRKGFNNVYDKALARALHKIFNKHADLFNDMPGEFNIRLAANA 300

Query: 301 KTVRDYDNALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPDRGIPRED 360
           KTVRDYDNALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPDRGIPRED
Sbjct: 301 KTVRDYDNALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPDRGIPRED 360

Query: 361 IKENPNCLLIVTPKGGHLGWVAGPGAPLGFPWTDPFVIDFLVYLENHTAKASASSIGLTP 420
           IKENPNCLLIVTPKGGHLGWVAGPGAPLGFPWTDPFVIDFLVYLENHTAKASASSIGLTP
Sbjct: 361 IKENPNCLLIVTPKGGHLGWVAGPGAPLGFPWTDPFVIDFLVYLENHTAKASASSIGLTP 420

Query: 421 AEIV 425
           AE++
Sbjct: 421 AEVM 424

BLAST of Bhi12G000082 vs. NCBI nr
Match: KAA0042649.1 (embryogenesis-associated protein EMB8 [Cucumis melo var. makuwa] >TYK06050.1 embryogenesis-associated protein EMB8 [Cucumis melo var. makuwa])

HSP 1 Score: 795.4 bits (2053), Expect = 2.4e-226
Identity = 384/425 (90.35%), Postives = 405/425 (95.29%), Query Frame = 0

Query: 1   MARAFLGSTHSSSPLIALRFSSFTPS-FITNQRRRSLRPTPATVLAMAELPSEKRIQPHP 60
           MARAFLGST SSSPLIALR SSFTPS F+TNQR RSLRPT  +V+AMAE+PSEKRI+PHP
Sbjct: 34  MARAFLGSTSSSSPLIALRISSFTPSFFLTNQRPRSLRPTTGSVVAMAEVPSEKRIKPHP 93

Query: 61  SLEVLGGGCDRFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPNVKLRRECLRAAD 120
           SLEV+GGGCD+FLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCP+V L RECLRAAD
Sbjct: 94  SLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPSVNLHRECLRAAD 153

Query: 121 NGTVALDWVAGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGC 180
           NGTVALDWV GDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGC
Sbjct: 154 NGTVALDWVTGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGC 213

Query: 181 GNSPVTTPQFYSASFLGDMREVVALVTQRYPKANVYAVGWSLGGNILVNYLGQDSLSCPL 240
           GNSPVTTPQFYSASFLGDMREVVA VT+RYPKANVYAVGWSLGGNILVNYLGQDSL+CPL
Sbjct: 214 GNSPVTTPQFYSASFLGDMREVVAHVTKRYPKANVYAVGWSLGGNILVNYLGQDSLTCPL 273

Query: 241 SGAVSLCNPFDLIVADEDFRKGFNNVYDKALARALHKIFNKHADLFNDMPGEFNIRLAAN 300
           SGAVSLCNPFDLIVADEDFRKGFNNVYDK+LARAL KIFNKHA LF DMP EFNIRLAA 
Sbjct: 274 SGAVSLCNPFDLIVADEDFRKGFNNVYDKSLARALRKIFNKHAALFTDMPSEFNIRLAAK 333

Query: 301 AKTVRDYDNALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPDRGIPRE 360
           A+TVRDYD+ALTRVSFGFN+VDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAP+RGIPRE
Sbjct: 334 ARTVRDYDDALTRVSFGFNTVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPNRGIPRE 393

Query: 361 DIKENPNCLLIVTPKGGHLGWVAGPGAPLGFPWTDPFVIDFLVYLENHTAKASASSIGLT 420
           DIKENPNC+LIVTP+GGHLGWVAGPGAPLGFPWTDPFVIDFL+YLEN  +KASASS  LT
Sbjct: 394 DIKENPNCMLIVTPRGGHLGWVAGPGAPLGFPWTDPFVIDFLMYLENQKSKASASSTDLT 453

Query: 421 PAEIV 425
            AE++
Sbjct: 454 TAEVM 458

BLAST of Bhi12G000082 vs. NCBI nr
Match: XP_008437445.1 (PREDICTED: LOW QUALITY PROTEIN: embryogenesis-associated protein EMB8 [Cucumis melo])

HSP 1 Score: 795.4 bits (2053), Expect = 2.4e-226
Identity = 384/425 (90.35%), Postives = 405/425 (95.29%), Query Frame = 0

Query: 1   MARAFLGSTHSSSPLIALRFSSFTPS-FITNQRRRSLRPTPATVLAMAELPSEKRIQPHP 60
           MARAFLGST SSSPLIALR SSFTPS F+TNQR RSLRPT  +V+AMAE+PSEKRI+PHP
Sbjct: 27  MARAFLGSTSSSSPLIALRISSFTPSFFLTNQRPRSLRPTTGSVVAMAEVPSEKRIKPHP 86

Query: 61  SLEVLGGGCDRFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPNVKLRRECLRAAD 120
           SLEV+GGGCD+FLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCP+V L RECLRAAD
Sbjct: 87  SLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPSVNLHRECLRAAD 146

Query: 121 NGTVALDWVAGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGC 180
           NGTVALDWV GDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGC
Sbjct: 147 NGTVALDWVTGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGC 206

Query: 181 GNSPVTTPQFYSASFLGDMREVVALVTQRYPKANVYAVGWSLGGNILVNYLGQDSLSCPL 240
           GNSPVTTPQFYSASFLGDMREVVA VT+RYPKANVYAVGWSLGGNILVNYLGQDSL+CPL
Sbjct: 207 GNSPVTTPQFYSASFLGDMREVVAHVTKRYPKANVYAVGWSLGGNILVNYLGQDSLTCPL 266

Query: 241 SGAVSLCNPFDLIVADEDFRKGFNNVYDKALARALHKIFNKHADLFNDMPGEFNIRLAAN 300
           SGAVSLCNPFDLIVADEDFRKGFNNVYDK+LARAL KIFNKHA LF DMP EFNIRLAA 
Sbjct: 267 SGAVSLCNPFDLIVADEDFRKGFNNVYDKSLARALRKIFNKHAALFTDMPSEFNIRLAAK 326

Query: 301 AKTVRDYDNALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPDRGIPRE 360
           A+TVRDYD+ALTRVSFGFN+VDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAP+RGIPRE
Sbjct: 327 ARTVRDYDDALTRVSFGFNTVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPNRGIPRE 386

Query: 361 DIKENPNCLLIVTPKGGHLGWVAGPGAPLGFPWTDPFVIDFLVYLENHTAKASASSIGLT 420
           DIKENPNC+LIVTP+GGHLGWVAGPGAPLGFPWTDPFVIDFL+YLEN  +KASASS  LT
Sbjct: 387 DIKENPNCMLIVTPRGGHLGWVAGPGAPLGFPWTDPFVIDFLMYLENQKSKASASSTDLT 446

Query: 421 PAEIV 425
            AE++
Sbjct: 447 TAEVM 451

BLAST of Bhi12G000082 vs. NCBI nr
Match: XP_004143840.3 (embryogenesis-associated protein EMB8 [Cucumis sativus] >KGN49619.2 hypothetical protein Csa_000306 [Cucumis sativus])

HSP 1 Score: 785.0 bits (2026), Expect = 3.2e-223
Identity = 383/425 (90.12%), Postives = 401/425 (94.35%), Query Frame = 0

Query: 1   MARAFLGSTHSSSPLIALRFSSFTPS-FITNQRRRSLRPTPATVLAMAELPSEKRIQPHP 60
           MARAFLGST SSSPLIALR SSFTPS F+TNQR RSLR T  TV+ MAELPSEKRI+PHP
Sbjct: 55  MARAFLGSTPSSSPLIALRLSSFTPSFFLTNQRPRSLRLTTDTVVPMAELPSEKRIKPHP 114

Query: 61  SLEVLGGGCDRFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPNVKLRRECLRAAD 120
           SLEV+GGGCD+FLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCP+V L RECLRAAD
Sbjct: 115 SLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPSVNLHRECLRAAD 174

Query: 121 NGTVALDWVAGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGC 180
           NGTVALDWVAGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGC
Sbjct: 175 NGTVALDWVAGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGC 234

Query: 181 GNSPVTTPQFYSASFLGDMREVVALVTQRYPKANVYAVGWSLGGNILVNYLGQDSLSCPL 240
           GNSPVTTPQFYSASFLGDMREVVA VT+RYPKAN+YAVGWSLGGNILVNYLGQDSL+CPL
Sbjct: 235 GNSPVTTPQFYSASFLGDMREVVAHVTERYPKANLYAVGWSLGGNILVNYLGQDSLTCPL 294

Query: 241 SGAVSLCNPFDLIVADEDFRKGFNNVYDKALARALHKIFNKHADLFNDMPGEFNIRLAAN 300
           SGAVSLCNPFDLIVADEDFRKGFNNVYDK+LA AL KIFNKHA LF DM GEFNIRLAA 
Sbjct: 295 SGAVSLCNPFDLIVADEDFRKGFNNVYDKSLAGALRKIFNKHAALFTDMTGEFNIRLAAK 354

Query: 301 AKTVRDYDNALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPDRGIPRE 360
           A TVRDYD+ALTRVSFGFN+VDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAP+RGIPRE
Sbjct: 355 ATTVRDYDDALTRVSFGFNTVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPNRGIPRE 414

Query: 361 DIKENPNCLLIVTPKGGHLGWVAGPGAPLGFPWTDPFVIDFLVYLENHTAKASASSIGLT 420
           DI+ENPNC+LIVTPKGGHLGWVAGPGAPLGFPWTDPFVIDFL+YLEN   KASASS  LT
Sbjct: 415 DIEENPNCMLIVTPKGGHLGWVAGPGAPLGFPWTDPFVIDFLMYLENKKFKASASSTDLT 474

Query: 421 PAEIV 425
            AE++
Sbjct: 475 MAEVM 479

BLAST of Bhi12G000082 vs. NCBI nr
Match: XP_022949852.1 (embryogenesis-associated protein EMB8-like [Cucurbita moschata])

HSP 1 Score: 772.7 bits (1994), Expect = 1.7e-219
Identity = 373/422 (88.39%), Postives = 393/422 (93.13%), Query Frame = 0

Query: 1   MARAFLGSTHSSSPLIALRFSSFTPSFITNQRRRSLRPTPATVLAMAELPSEKRIQPHPS 60
           MARAFLGST SSS L   R SSFTPS I NQRRRSLR T A V+AM+E PSEK IQPHPS
Sbjct: 28  MARAFLGSTPSSSLLTTSRLSSFTPSLINNQRRRSLRLTTAAVVAMSEFPSEKPIQPHPS 87

Query: 61  LEVLGGGCDRFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPNVKLRRECLRAADN 120
           LEV+GGGCDRFLPA KDL+LPYKPFPVIG NRHLETIFASF+R+CP+V+ RRECLRAADN
Sbjct: 88  LEVIGGGCDRFLPALKDLNLPYKPFPVIGWNRHLETIFASFYRSCPDVRFRRECLRAADN 147

Query: 121 GTVALDWVAGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCG 180
           GTVALDWV GDDLRLP+D+PVLILLPGLTGGSQDSYVRHMLVRARDRGWR VVFNSRGCG
Sbjct: 148 GTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQDSYVRHMLVRARDRGWRAVVFNSRGCG 207

Query: 181 NSPVTTPQFYSASFLGDMREVVALVTQRYPKANVYAVGWSLGGNILVNYLGQDSLSCPLS 240
           NSPVTTPQFYSASFLGDMREVV  VT+RYPKANVYAVGWSLGGNILVNYLGQDSLSCPLS
Sbjct: 208 NSPVTTPQFYSASFLGDMREVVEHVTKRYPKANVYAVGWSLGGNILVNYLGQDSLSCPLS 267

Query: 241 GAVSLCNPFDLIVADEDFRKGFNNVYDKALARALHKIFNKHADLFNDMPGEFNIRLAANA 300
           GAVSLCNPFDL+VADEDFRKGFNNVYDKALARAL +IF+KHADLFNDMPGEFNI LAANA
Sbjct: 268 GAVSLCNPFDLVVADEDFRKGFNNVYDKALARALRRIFSKHADLFNDMPGEFNIPLAANA 327

Query: 301 KTVRDYDNALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPDRGIPRED 360
           +TVR+YD+ALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPDRG+PRED
Sbjct: 328 RTVREYDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPDRGVPRED 387

Query: 361 IKENPNCLLIVTPKGGHLGWVAGPGAPLGFPWTDPFVIDFLVYLENHTAKASASSIGLTP 420
           IKENPNCLLIVTPKGGHLGWVAGP APLG PWTDPFVIDFLVYLE+  +KASASS  LT 
Sbjct: 388 IKENPNCLLIVTPKGGHLGWVAGPSAPLGNPWTDPFVIDFLVYLESQESKASASSTDLTA 447

Query: 421 AE 423
           AE
Sbjct: 448 AE 449

BLAST of Bhi12G000082 vs. ExPASy TrEMBL
Match: A0A5A7TMR0 (Embryogenesis-associated protein EMB8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001510 PE=4 SV=1)

HSP 1 Score: 795.4 bits (2053), Expect = 1.2e-226
Identity = 384/425 (90.35%), Postives = 405/425 (95.29%), Query Frame = 0

Query: 1   MARAFLGSTHSSSPLIALRFSSFTPS-FITNQRRRSLRPTPATVLAMAELPSEKRIQPHP 60
           MARAFLGST SSSPLIALR SSFTPS F+TNQR RSLRPT  +V+AMAE+PSEKRI+PHP
Sbjct: 34  MARAFLGSTSSSSPLIALRISSFTPSFFLTNQRPRSLRPTTGSVVAMAEVPSEKRIKPHP 93

Query: 61  SLEVLGGGCDRFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPNVKLRRECLRAAD 120
           SLEV+GGGCD+FLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCP+V L RECLRAAD
Sbjct: 94  SLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPSVNLHRECLRAAD 153

Query: 121 NGTVALDWVAGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGC 180
           NGTVALDWV GDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGC
Sbjct: 154 NGTVALDWVTGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGC 213

Query: 181 GNSPVTTPQFYSASFLGDMREVVALVTQRYPKANVYAVGWSLGGNILVNYLGQDSLSCPL 240
           GNSPVTTPQFYSASFLGDMREVVA VT+RYPKANVYAVGWSLGGNILVNYLGQDSL+CPL
Sbjct: 214 GNSPVTTPQFYSASFLGDMREVVAHVTKRYPKANVYAVGWSLGGNILVNYLGQDSLTCPL 273

Query: 241 SGAVSLCNPFDLIVADEDFRKGFNNVYDKALARALHKIFNKHADLFNDMPGEFNIRLAAN 300
           SGAVSLCNPFDLIVADEDFRKGFNNVYDK+LARAL KIFNKHA LF DMP EFNIRLAA 
Sbjct: 274 SGAVSLCNPFDLIVADEDFRKGFNNVYDKSLARALRKIFNKHAALFTDMPSEFNIRLAAK 333

Query: 301 AKTVRDYDNALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPDRGIPRE 360
           A+TVRDYD+ALTRVSFGFN+VDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAP+RGIPRE
Sbjct: 334 ARTVRDYDDALTRVSFGFNTVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPNRGIPRE 393

Query: 361 DIKENPNCLLIVTPKGGHLGWVAGPGAPLGFPWTDPFVIDFLVYLENHTAKASASSIGLT 420
           DIKENPNC+LIVTP+GGHLGWVAGPGAPLGFPWTDPFVIDFL+YLEN  +KASASS  LT
Sbjct: 394 DIKENPNCMLIVTPRGGHLGWVAGPGAPLGFPWTDPFVIDFLMYLENQKSKASASSTDLT 453

Query: 421 PAEIV 425
            AE++
Sbjct: 454 TAEVM 458

BLAST of Bhi12G000082 vs. ExPASy TrEMBL
Match: A0A1S3AU57 (LOW QUALITY PROTEIN: embryogenesis-associated protein EMB8 OS=Cucumis melo OX=3656 GN=LOC103482859 PE=4 SV=1)

HSP 1 Score: 795.4 bits (2053), Expect = 1.2e-226
Identity = 384/425 (90.35%), Postives = 405/425 (95.29%), Query Frame = 0

Query: 1   MARAFLGSTHSSSPLIALRFSSFTPS-FITNQRRRSLRPTPATVLAMAELPSEKRIQPHP 60
           MARAFLGST SSSPLIALR SSFTPS F+TNQR RSLRPT  +V+AMAE+PSEKRI+PHP
Sbjct: 27  MARAFLGSTSSSSPLIALRISSFTPSFFLTNQRPRSLRPTTGSVVAMAEVPSEKRIKPHP 86

Query: 61  SLEVLGGGCDRFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPNVKLRRECLRAAD 120
           SLEV+GGGCD+FLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCP+V L RECLRAAD
Sbjct: 87  SLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPSVNLHRECLRAAD 146

Query: 121 NGTVALDWVAGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGC 180
           NGTVALDWV GDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGC
Sbjct: 147 NGTVALDWVTGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGC 206

Query: 181 GNSPVTTPQFYSASFLGDMREVVALVTQRYPKANVYAVGWSLGGNILVNYLGQDSLSCPL 240
           GNSPVTTPQFYSASFLGDMREVVA VT+RYPKANVYAVGWSLGGNILVNYLGQDSL+CPL
Sbjct: 207 GNSPVTTPQFYSASFLGDMREVVAHVTKRYPKANVYAVGWSLGGNILVNYLGQDSLTCPL 266

Query: 241 SGAVSLCNPFDLIVADEDFRKGFNNVYDKALARALHKIFNKHADLFNDMPGEFNIRLAAN 300
           SGAVSLCNPFDLIVADEDFRKGFNNVYDK+LARAL KIFNKHA LF DMP EFNIRLAA 
Sbjct: 267 SGAVSLCNPFDLIVADEDFRKGFNNVYDKSLARALRKIFNKHAALFTDMPSEFNIRLAAK 326

Query: 301 AKTVRDYDNALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPDRGIPRE 360
           A+TVRDYD+ALTRVSFGFN+VDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAP+RGIPRE
Sbjct: 327 ARTVRDYDDALTRVSFGFNTVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPNRGIPRE 386

Query: 361 DIKENPNCLLIVTPKGGHLGWVAGPGAPLGFPWTDPFVIDFLVYLENHTAKASASSIGLT 420
           DIKENPNC+LIVTP+GGHLGWVAGPGAPLGFPWTDPFVIDFL+YLEN  +KASASS  LT
Sbjct: 387 DIKENPNCMLIVTPRGGHLGWVAGPGAPLGFPWTDPFVIDFLMYLENQKSKASASSTDLT 446

Query: 421 PAEIV 425
            AE++
Sbjct: 447 TAEVM 451

BLAST of Bhi12G000082 vs. ExPASy TrEMBL
Match: A0A0A0KNN2 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G148530 PE=4 SV=1)

HSP 1 Score: 785.0 bits (2026), Expect = 1.6e-223
Identity = 383/425 (90.12%), Postives = 401/425 (94.35%), Query Frame = 0

Query: 1   MARAFLGSTHSSSPLIALRFSSFTPS-FITNQRRRSLRPTPATVLAMAELPSEKRIQPHP 60
           MARAFLGST SSSPLIALR SSFTPS F+TNQR RSLR T  TV+ MAELPSEKRI+PHP
Sbjct: 55  MARAFLGSTPSSSPLIALRLSSFTPSFFLTNQRPRSLRLTTDTVVPMAELPSEKRIKPHP 114

Query: 61  SLEVLGGGCDRFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPNVKLRRECLRAAD 120
           SLEV+GGGCD+FLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCP+V L RECLRAAD
Sbjct: 115 SLEVIGGGCDQFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPSVNLHRECLRAAD 174

Query: 121 NGTVALDWVAGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGC 180
           NGTVALDWVAGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGC
Sbjct: 175 NGTVALDWVAGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGC 234

Query: 181 GNSPVTTPQFYSASFLGDMREVVALVTQRYPKANVYAVGWSLGGNILVNYLGQDSLSCPL 240
           GNSPVTTPQFYSASFLGDMREVVA VT+RYPKAN+YAVGWSLGGNILVNYLGQDSL+CPL
Sbjct: 235 GNSPVTTPQFYSASFLGDMREVVAHVTERYPKANLYAVGWSLGGNILVNYLGQDSLTCPL 294

Query: 241 SGAVSLCNPFDLIVADEDFRKGFNNVYDKALARALHKIFNKHADLFNDMPGEFNIRLAAN 300
           SGAVSLCNPFDLIVADEDFRKGFNNVYDK+LA AL KIFNKHA LF DM GEFNIRLAA 
Sbjct: 295 SGAVSLCNPFDLIVADEDFRKGFNNVYDKSLAGALRKIFNKHAALFTDMTGEFNIRLAAQ 354

Query: 301 AKTVRDYDNALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPDRGIPRE 360
           A TVRDYD+ALTRVSFGFN+VDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAP+RGIPRE
Sbjct: 355 ATTVRDYDDALTRVSFGFNTVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPNRGIPRE 414

Query: 361 DIKENPNCLLIVTPKGGHLGWVAGPGAPLGFPWTDPFVIDFLVYLENHTAKASASSIGLT 420
           DI+ENPNC+LIVTPKGGHLGWVAGPGAPLGFPWTDPFVIDFL+YLEN   KASASS  LT
Sbjct: 415 DIEENPNCMLIVTPKGGHLGWVAGPGAPLGFPWTDPFVIDFLMYLENKKFKASASSTDLT 474

Query: 421 PAEIV 425
            AE++
Sbjct: 475 MAEVM 479

BLAST of Bhi12G000082 vs. ExPASy TrEMBL
Match: A0A6J1GD89 (embryogenesis-associated protein EMB8-like OS=Cucurbita moschata OX=3662 GN=LOC111453123 PE=4 SV=1)

HSP 1 Score: 772.7 bits (1994), Expect = 8.1e-220
Identity = 373/422 (88.39%), Postives = 393/422 (93.13%), Query Frame = 0

Query: 1   MARAFLGSTHSSSPLIALRFSSFTPSFITNQRRRSLRPTPATVLAMAELPSEKRIQPHPS 60
           MARAFLGST SSS L   R SSFTPS I NQRRRSLR T A V+AM+E PSEK IQPHPS
Sbjct: 28  MARAFLGSTPSSSLLTTSRLSSFTPSLINNQRRRSLRLTTAAVVAMSEFPSEKPIQPHPS 87

Query: 61  LEVLGGGCDRFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPNVKLRRECLRAADN 120
           LEV+GGGCDRFLPA KDL+LPYKPFPVIG NRHLETIFASF+R+CP+V+ RRECLRAADN
Sbjct: 88  LEVIGGGCDRFLPALKDLNLPYKPFPVIGWNRHLETIFASFYRSCPDVRFRRECLRAADN 147

Query: 121 GTVALDWVAGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCG 180
           GTVALDWV GDDLRLP+D+PVLILLPGLTGGSQDSYVRHMLVRARDRGWR VVFNSRGCG
Sbjct: 148 GTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQDSYVRHMLVRARDRGWRAVVFNSRGCG 207

Query: 181 NSPVTTPQFYSASFLGDMREVVALVTQRYPKANVYAVGWSLGGNILVNYLGQDSLSCPLS 240
           NSPVTTPQFYSASFLGDMREVV  VT+RYPKANVYAVGWSLGGNILVNYLGQDSLSCPLS
Sbjct: 208 NSPVTTPQFYSASFLGDMREVVEHVTKRYPKANVYAVGWSLGGNILVNYLGQDSLSCPLS 267

Query: 241 GAVSLCNPFDLIVADEDFRKGFNNVYDKALARALHKIFNKHADLFNDMPGEFNIRLAANA 300
           GAVSLCNPFDL+VADEDFRKGFNNVYDKALARAL +IF+KHADLFNDMPGEFNI LAANA
Sbjct: 268 GAVSLCNPFDLVVADEDFRKGFNNVYDKALARALRRIFSKHADLFNDMPGEFNIPLAANA 327

Query: 301 KTVRDYDNALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPDRGIPRED 360
           +TVR+YD+ALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPDRG+PRED
Sbjct: 328 RTVREYDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPDRGVPRED 387

Query: 361 IKENPNCLLIVTPKGGHLGWVAGPGAPLGFPWTDPFVIDFLVYLENHTAKASASSIGLTP 420
           IKENPNCLLIVTPKGGHLGWVAGP APLG PWTDPFVIDFLVYLE+  +KASASS  LT 
Sbjct: 388 IKENPNCLLIVTPKGGHLGWVAGPSAPLGNPWTDPFVIDFLVYLESQESKASASSTDLTA 447

Query: 421 AE 423
           AE
Sbjct: 448 AE 449

BLAST of Bhi12G000082 vs. ExPASy TrEMBL
Match: A0A6J1IJD9 (embryogenesis-associated protein EMB8-like OS=Cucurbita maxima OX=3661 GN=LOC111477973 PE=4 SV=1)

HSP 1 Score: 767.7 bits (1981), Expect = 2.6e-218
Identity = 372/422 (88.15%), Postives = 392/422 (92.89%), Query Frame = 0

Query: 1   MARAFLGSTHSSSPLIALRFSSFTPSFITNQRRRSLRPTPATVLAMAELPSEKRIQPHPS 60
           MARAFLGST SSS L   R SSFTPS ITNQRRRSLR T A V+AM+E PSEK IQPHPS
Sbjct: 28  MARAFLGSTPSSSLLTTSRLSSFTPSLITNQRRRSLRLTTAAVVAMSEFPSEKPIQPHPS 87

Query: 61  LEVLGGGCDRFLPAFKDLDLPYKPFPVIGSNRHLETIFASFFRTCPNVKLRRECLRAADN 120
           LEV+GGG DRFLPA KDL+LPYKPFPVIG NRHLETIFASF+R+CP+V+ RRECLRAADN
Sbjct: 88  LEVIGGGRDRFLPALKDLNLPYKPFPVIGWNRHLETIFASFYRSCPDVRFRRECLRAADN 147

Query: 121 GTVALDWVAGDDLRLPLDSPVLILLPGLTGGSQDSYVRHMLVRARDRGWRVVVFNSRGCG 180
           GTVALDWV GDDLRLP+D+PVLILLPGLTGGSQDSYVRHMLVRARDRGWR VVFNSRGCG
Sbjct: 148 GTVALDWVVGDDLRLPIDAPVLILLPGLTGGSQDSYVRHMLVRARDRGWRAVVFNSRGCG 207

Query: 181 NSPVTTPQFYSASFLGDMREVVALVTQRYPKANVYAVGWSLGGNILVNYLGQDSLSCPLS 240
           NSPVTTPQFYSASFLGDMREVV  VT+RYPKANVYAVGWSLGGNILVNYLGQDSLSCPLS
Sbjct: 208 NSPVTTPQFYSASFLGDMREVVEHVTKRYPKANVYAVGWSLGGNILVNYLGQDSLSCPLS 267

Query: 241 GAVSLCNPFDLIVADEDFRKGFNNVYDKALARALHKIFNKHADLFNDMPGEFNIRLAANA 300
           GAVSLCNPFDL+VADEDFRKGFNNVYDKALARAL +IF+KHADLFNDMPGEFNI LAANA
Sbjct: 268 GAVSLCNPFDLVVADEDFRKGFNNVYDKALARALRRIFSKHADLFNDMPGEFNIPLAANA 327

Query: 301 KTVRDYDNALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPDRGIPRED 360
           +TVR+YD+ALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPDRG+PRED
Sbjct: 328 RTVREYDDALTRVSFGFNSVDEYYSKSSSSKFIKDVKIPLLCIQAANDPIAPDRGVPRED 387

Query: 361 IKENPNCLLIVTPKGGHLGWVAGPGAPLGFPWTDPFVIDFLVYLENHTAKASASSIGLTP 420
           IK NPNCLLIVTPKGGHLGWVAGP APLG PWTDPFVIDFLVYLE+  +KASASS  LT 
Sbjct: 388 IKANPNCLLIVTPKGGHLGWVAGPSAPLGNPWTDPFVIDFLVYLESQESKASASSTDLTA 447

Query: 421 AE 423
           AE
Sbjct: 448 AE 449

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT3G50790.17.1e-16070.10esterase/lipase/thioesterase family protein [more]
AT5G49950.16.6e-5735.50alpha/beta-Hydrolases superfamily protein [more]
AT1G34340.13.1e-5434.97alpha/beta-Hydrolases superfamily protein [more]
AT2G03140.16.6e-4133.22alpha/beta-Hydrolases superfamily protein [more]
AT2G03140.26.6e-4133.22alpha/beta-Hydrolases superfamily protein [more]
Match NameE-valueIdentityDescription
Q408634.8e-12965.14Embryogenesis-associated protein EMB8 OS=Picea glauca OX=3330 GN=EMB8 PE=2 SV=1[more]
Q0VC003.1e-5134.47Phospholipase ABHD3 OS=Bos taurus OX=9913 GN=ABHD3 PE=2 SV=1[more]
Q8WU671.2e-5034.47Phospholipase ABHD3 OS=Homo sapiens OX=9606 GN=ABHD3 PE=2 SV=2[more]
Q91ZH71.7e-4933.79Phospholipase ABHD3 OS=Mus musculus OX=10090 GN=Abhd3 PE=1 SV=1[more]
Q54H383.5e-4734.03Abhydrolase domain-containing protein OS=Dictyostelium discoideum OX=44689 GN=ab... [more]
Match NameE-valueIdentityDescription
XP_038875981.12.0e-24999.53embryogenesis-associated protein EMB8 [Benincasa hispida][more]
KAA0042649.12.4e-22690.35embryogenesis-associated protein EMB8 [Cucumis melo var. makuwa] >TYK06050.1 emb... [more]
XP_008437445.12.4e-22690.35PREDICTED: LOW QUALITY PROTEIN: embryogenesis-associated protein EMB8 [Cucumis m... [more]
XP_004143840.33.2e-22390.12embryogenesis-associated protein EMB8 [Cucumis sativus] >KGN49619.2 hypothetical... [more]
XP_022949852.11.7e-21988.39embryogenesis-associated protein EMB8-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A5A7TMR01.2e-22690.35Embryogenesis-associated protein EMB8 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
A0A1S3AU571.2e-22690.35LOW QUALITY PROTEIN: embryogenesis-associated protein EMB8 OS=Cucumis melo OX=36... [more]
A0A0A0KNN21.6e-22390.12AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G148... [more]
A0A6J1GD898.1e-22088.39embryogenesis-associated protein EMB8-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1IJD92.6e-21888.15embryogenesis-associated protein EMB8-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PFAMPF00561Abhydrolase_1coord: 140..378
e-value: 7.5E-22
score: 78.2
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 112..390
e-value: 6.0E-25
score: 90.0
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 101..387
NoneNo IPR availablePANTHERPTHR10794:SF81EMBRYOGENESIS-ASSOCIATED PROTEIN EMB8-LIKEcoord: 37..412
NoneNo IPR availablePANTHERPTHR10794ABHYDROLASE DOMAIN-CONTAINING PROTEINcoord: 37..412
IPR000952AB hydrolase 4, conserved sitePROSITEPS01133UPF0017coord: 307..348

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi12M000082Bhi12M000082mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0044255 cellular lipid metabolic process
molecular_function GO:0047372 acylglycerol lipase activity
molecular_function GO:0034338 short-chain carboxylesterase activity