Bhi12G000008 (gene) Wax gourd (B227) v1

Overview
NameBhi12G000008
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionMannose-6-phosphate isomerase
Locationchr12: 274661 .. 278648 (+)
RNA-Seq ExpressionBhi12G000008
SyntenyBhi12G000008
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCAGTTTCTTCCTTCGGCGATTTCCCTTCACTCCCTCAGGCTTGAACACCGTCATCTCCGTTCTCACATTCTCCCTCGCGTCGGTTATCTTATCTCCTTCACCTCACCGCTAACTACTTTGCTCCCTCGCCGTCGATCTTCCTCTGGCCGTCGATCTCCGTCACGCCGTCTCTCTCATTTCAGTCCATCGATCTCCCTCACGGCGTCTCTCTCCTCCAGTCCGTCGATCTCCAGTGGTACTGAGTTCAAACTCCAAACGTAACTCTTCACTCTTCCATCTTTCTCTGTGTTTCTCTGTTTTTTCCATTTCACATCTCAGATCTCAATTTCTGATCTCTTACATTCAGCGTCTCAGGCTTAGCTATTCTCGATTTCTAGCGGCGTTTTGGGCGGGAGGCGGATGGGAGCAGAGTGGGCACGAGAACGAGGTTAAACGAGGAAGAGTTTCTTTTCTTTTCTTTTTTTTTTTAGATATGGCTCTGATACCACATCTGTGAATCTTGGCCTAATTTGGAGTCTAATTTGACAAATTTACAGCATATGGAAAGGATTACGTTAACAACCTCCATTCGTGTGCCTCTCGTTGATGACCAATGGACGAGGAAGATGATAACAAATTGAAGAAGTGTAGGAAGAAGAGACTGTCAGGCGCCGACAGGAGGAAGCGGCTCCTTCGTCTCAAATGTTGTGTTCAAAACTACGATTGGGGGGTTATTGGGTGTAATTCCCAAGTCGCGAGGCTGTTTTTGCTCAATTCAGGTTGCTGCAGTCTCGATCCGAGGGAATGTTACGCCGAATTTTGGATTGGGACGCATAAATCGGGACCGTCTTTTGTTGTTTTTGGACGGGACAATAATGCCGGCGCTGCGTTTGGATCCAAACCCCTGAGTCTCAAGGATTGGATCTCCGTCGATCCGGGTGCTGTGCTTGGTGATAAAGTTGCGAGGAAATGGGGAGGCGATCTTCCCTTTCTCTTCAAGGTCCGTTTTCTTTTCCATTAGATCCTTGGGGAATGTGTTTTGTCGGTGTGCTTGATTTCGTTTAAGATTTCACAATGGGACAGGAATTTTGGGTTTTAATTATGGTCTACATTGTGTCGGTGTGTTAGTTAATTCGGATGCTGAGATACTGTTGAATGACATTCATGCTTCATAAACTAAAATTATGTGCTGCAGCTCTTTGAATTGCATGTTAAATTAGTTTCTCGAATCTCAATTACCGTTCTTATTTTCCTTGATGTTAATGTTTTGAGCTCAGTTGGACTGCAACTTGATGATTTCTTATTGGGAGCATAGGTGCTGTCGATAGAGAAGGCTTTATCTATTCAGGCTCATCCAGATAAAGATTTGGCGAGATCCTTGAACCAAGCTCAACCAAGCATCTATAAAGATGATAATCACAAGCCTGAAATGGCTCTTGCATTGACTGGATTTGAGGCCCTCTGTGGGTTCATCAGCTCTAAGGTACCACAACATATAGTAGCAAAGGGTAGTTCTTTTGGGGTTTTTGAAAAGTTTCTTAAAATTATTTTAAGCACAAAAATCCTTGTTTTTCCTGTTAGTTAAGTGAAAATATCTTAATAATTCTTACTCTGTTCGTATTACACTAATGGGTTTAGAGAGATGAAAGAAACTCAAATCAAGATGTTGATTCAGCTTTTTGGTTAATTGTGCATGTATTACCAGATGAGTACATTCAGAAAAATAATCGAAGAATATAAGTAACAGATGATGAATGAAGTTTCCTTATAAATTCCTCTACTTTGCAATATCCATGTCATTTGATTTTACTTATTTCATTATTTTCCAGGAGCTCAAGGTGGTGCTTAGTAGCGTTCCTGAGATAGTAGAATTAGTCCAATGTGCAGATGCAGAGAAGTTCTCACATGAAAGTGAGCAAGATGGGAAGGAGAAAGTAAAACAGCTTTTTGAGTCTATATTTAGTCAGATCATGTCATCTAACAAGGGCATAGTTCGCGAGGCTATATGTAAACTAAAAAGGCGTCTTAGTTTGGAGAAAAAGGTAACTGAAGTTTTATATCTCTGCAAATTATCTTGTCAGCAGGATTTGGCATTTTTCATTGTTATATATTTTAAACCTCAAGGCCCATCATTAGCAAGTTCTATGATTGAAAGGATACATTTCATGATAAACCTAAGTTGTTCTCTCACTTCATCAGTCTCTGGAAAGTATTATTCATCTGCTTTCACATTGTTTTATGCAAGTTCTCATTGTATTACATTCTTTGTTTTTTTTCTTTTCATCAGAAGAGACAATTATCTGCCAAAGAACAATTGATTCTCAGACTAGAAGGTCAATATCCTGCTGATGTTGGTATACTAGCAGCATTCTTTCTCAACTATGTGGAGTTAAAACCAGGCGAAGCATTATATGTTGGTCCAAATGAACCCCATGCATATATATCTGGGGAATGCATCGAGTGTATGGCAACCTCTGACAATGTTGTTCGTGCCGGCCTTACCTCCAAAAAAAGAGACGTTCAAGCACTTCTTTCAATGCTCAACTACAAACAGGTAAATCACAGAATGTATGCAGGAGGTGGTTTTTTATAATTATGCGATTCTAATGTGTAAACCGATTATATGCTATTGGTTCTTTTTTTCCATGTCCTCTATCTTAAAGTCTACAGGCTTATTAAAATTAATTCGTTTGCATTTTTTATTTTGACCGTAGTCTTAAATGCCACATTATATGCTGTCACTGTTCACAATAAGGTTTTCATATTGGTTGGAGCAATGTACATCGTTCTATAACTCTGATAGTCTTACTTCTCCCATTCTAGCGCTTGCTGCAGAAAGTAGTTAAAAATTGCCTCAGTAGTACGATCTTGAACGACTTGATTCATATTGATCTACCTTTATTTACCATTGGGAATAGGGGCAAATAGTTGGAGTCTTTTGTCTTTGTTTTTACCTCCTCTAATCTGCCCTGTCCAGTCCTTGAGAAGATTGCTCACATAATCAGTTCCTTCAACGTGCAGGGTTTTCCTGAAATTTTGCAAGGAGTTTCCTTGAATGAGTACACGAGAAAGTACGCCCCTCCCTTCGATGAATTCGAAGTTGATCGCTGTATTCTTCCACAAGCTGCATCTGTGTCCTTCCCATCTGTCCCAGGACCATCTCTCTTTCTAGTGATGTCTGGAAAAGGGACAATTGTGACAGGCTATTCTGAAGAAATTACATTTCAAGAGGGTGAAGTTCTGTTTGTTCCAGCATATATGGAGGTCAGTATAACAGCTGCAGCTATAGAATTACATATGTACAGAGCTGGGGTCAACAACAGATTCTTTGGAGACTTGTGATTTTCAGGTACAAATGAATAAATGAAGAATATACAGGAAGAAAAGAGGCTATAAGAAGAAAGAACTGTTCAAGTACACACAAAATTGTTGCATTTATTGTCCTTTATGATCAGTTTATTTTTATTGATTAAATTGCATCAAGGTTGTGCATATATGTTTAAGAATATATGAAAGGGTATCACGCTGTATTCAAGTTCATGATTGAATTGAGCAATATATTGTGCCGCATTTATTTTTGTCCCATATCAGCTATCAGAAGTGGATATTCACACAGTTCTTTGTTTGGGAGCATTAAATTTTAGGCTCCCTTCCCCATCCTCATTTCAGGAGATCAAGAGGGCACAAGGGGTTTTGAAGTGGGTACTTTGTTTTTATTTTTATTATTTATTTATTATTATTATTTCGTGGGATAAGGCCATTTGGTAATTGTATTTTGATTTCTTATGAGAAACAGATGGGTTTTGCGGTGTTGTATAATTGTTGTATCATTATTTCTTCTAAGCTTGCTCAAATCTGTGTACAAGTTTTGAAATAAAAAAGAAGCTACCGGCATGTAATGTTATTGGATTTTTGTTGGCACCTATGCTAAAAAAATCTATAGTTTCAATTTTGAAGGTGGTAAAGCAGAG

mRNA sequence

TTCAGTTTCTTCCTTCGGCGATTTCCCTTCACTCCCTCAGGCTTGAACACCGTCATCTCCGTTCTCACATTCTCCCTCGCGTCGGTTATCTTATCTCCTTCACCTCACCGCTAACTACTTTGCTCCCTCGCCGTCGATCTTCCTCTGGCCGTCGATCTCCGTCACGCCGTCTCTCTCATTTCAGTCCATCGATCTCCCTCACGGCGTCTCTCTCCTCCAGTCCGTCGATCTCCAGTGGTACTGAGTTCAAACTCCAAACGCTTAGCTATTCTCGATTTCTAGCGGCGTTTTGGGCGGGAGGCGGATGGGAGCAGAGTGGGCACGAGAACGAGCATATGGAAAGGATTACGTTAACAACCTCCATTCGTGTGCCTCTCGTTGATGACCAATGGACGAGGAAGATGATAACAAATTGAAGAAGTGTAGGAAGAAGAGACTGTCAGGCGCCGACAGGAGGAAGCGGCTCCTTCGTCTCAAATGTTGTGTTCAAAACTACGATTGGGGGGTTATTGGGTGTAATTCCCAAGTCGCGAGGCTGTTTTTGCTCAATTCAGGTTGCTGCAGTCTCGATCCGAGGGAATGTTACGCCGAATTTTGGATTGGGACGCATAAATCGGGACCGTCTTTTGTTGTTTTTGGACGGGACAATAATGCCGGCGCTGCGTTTGGATCCAAACCCCTGAGTCTCAAGGATTGGATCTCCGTCGATCCGGGTGCTGTGCTTGGTGATAAAGTTGCGAGGAAATGGGGAGGCGATCTTCCCTTTCTCTTCAAGGTGCTGTCGATAGAGAAGGCTTTATCTATTCAGGCTCATCCAGATAAAGATTTGGCGAGATCCTTGAACCAAGCTCAACCAAGCATCTATAAAGATGATAATCACAAGCCTGAAATGGCTCTTGCATTGACTGGATTTGAGGCCCTCTGTGGGTTCATCAGCTCTAAGGAGCTCAAGGTGGTGCTTAGTAGCGTTCCTGAGATAGTAGAATTAGTCCAATGTGCAGATGCAGAGAAGTTCTCACATGAAAGTGAGCAAGATGGGAAGGAGAAAGTAAAACAGCTTTTTGAGTCTATATTTAGTCAGATCATGTCATCTAACAAGGGCATAGTTCGCGAGGCTATATGTAAACTAAAAAGGCGTCTTAGTTTGGAGAAAAAGAAGAGACAATTATCTGCCAAAGAACAATTGATTCTCAGACTAGAAGGTCAATATCCTGCTGATGTTGGTATACTAGCAGCATTCTTTCTCAACTATGTGGAGTTAAAACCAGGCGAAGCATTATATGTTGGTCCAAATGAACCCCATGCATATATATCTGGGGAATGCATCGAGTGTATGGCAACCTCTGACAATGTTGTTCGTGCCGGCCTTACCTCCAAAAAAAGAGACGTTCAAGCACTTCTTTCAATGCTCAACTACAAACAGGGTTTTCCTGAAATTTTGCAAGGAGTTTCCTTGAATGAGTACACGAGAAAGTACGCCCCTCCCTTCGATGAATTCGAAGTTGATCGCTGTATTCTTCCACAAGCTGCATCTGTGTCCTTCCCATCTGTCCCAGGACCATCTCTCTTTCTAGTGATGTCTGGAAAAGGGACAATTGTGACAGGCTATTCTGAAGAAATTACATTTCAAGAGGGTGAAGTTCTGTTTGTTCCAGCATATATGGAGGTCAGTATAACAGCTGCAGCTATAGAATTACATATGTACAGAGCTGGGGTCAACAACAGATTCTTTGGAGACTTGTGATTTTCAGGTACAAATGAATAAATGAAGAATATACAGGAAGAAAAGAGGCTATAAGAAGAAAGAACTGTTCAAGTACACACAAAATTGTTGCATTTATTGTCCTTTATGATCAGTTTATTTTTATTGATTAAATTGCATCAAGGTTGTGCATATATGTTTAAGAATATATGAAAGGGTATCACGCTGTATTCAAGTTCATGATTGAATTGAGCAATATATTGTGCCGCATTTATTTTTGTCCCATATCAGCTATCAGAAGTGGATATTCACACAGTTCTTTGTTTGGGAGCATTAAATTTTAGGCTCCCTTCCCCATCCTCATTTCAGGAGATCAAGAGGGCACAAGGGGTTTTGAAGTGGGTACTTTGTTTTTATTTTTATTATTTATTTATTATTATTATTTCGTGGGATAAGGCCATTTGGTAATTGTATTTTGATTTCTTATGAGAAACAGATGGGTTTTGCGGTGTTGTATAATTGTTGTATCATTATTTCTTCTAAGCTTGCTCAAATCTGTGTACAAGTTTTGAAATAAAAAAGAAGCTACCGGCATGTAATGTTATTGGATTTTTGTTGGCACCTATGCTAAAAAAATCTATAGTTTCAATTTTGAAGGTGGTAAAGCAGAG

Coding sequence (CDS)

ATGGACGAGGAAGATGATAACAAATTGAAGAAGTGTAGGAAGAAGAGACTGTCAGGCGCCGACAGGAGGAAGCGGCTCCTTCGTCTCAAATGTTGTGTTCAAAACTACGATTGGGGGGTTATTGGGTGTAATTCCCAAGTCGCGAGGCTGTTTTTGCTCAATTCAGGTTGCTGCAGTCTCGATCCGAGGGAATGTTACGCCGAATTTTGGATTGGGACGCATAAATCGGGACCGTCTTTTGTTGTTTTTGGACGGGACAATAATGCCGGCGCTGCGTTTGGATCCAAACCCCTGAGTCTCAAGGATTGGATCTCCGTCGATCCGGGTGCTGTGCTTGGTGATAAAGTTGCGAGGAAATGGGGAGGCGATCTTCCCTTTCTCTTCAAGGTGCTGTCGATAGAGAAGGCTTTATCTATTCAGGCTCATCCAGATAAAGATTTGGCGAGATCCTTGAACCAAGCTCAACCAAGCATCTATAAAGATGATAATCACAAGCCTGAAATGGCTCTTGCATTGACTGGATTTGAGGCCCTCTGTGGGTTCATCAGCTCTAAGGAGCTCAAGGTGGTGCTTAGTAGCGTTCCTGAGATAGTAGAATTAGTCCAATGTGCAGATGCAGAGAAGTTCTCACATGAAAGTGAGCAAGATGGGAAGGAGAAAGTAAAACAGCTTTTTGAGTCTATATTTAGTCAGATCATGTCATCTAACAAGGGCATAGTTCGCGAGGCTATATGTAAACTAAAAAGGCGTCTTAGTTTGGAGAAAAAGAAGAGACAATTATCTGCCAAAGAACAATTGATTCTCAGACTAGAAGGTCAATATCCTGCTGATGTTGGTATACTAGCAGCATTCTTTCTCAACTATGTGGAGTTAAAACCAGGCGAAGCATTATATGTTGGTCCAAATGAACCCCATGCATATATATCTGGGGAATGCATCGAGTGTATGGCAACCTCTGACAATGTTGTTCGTGCCGGCCTTACCTCCAAAAAAAGAGACGTTCAAGCACTTCTTTCAATGCTCAACTACAAACAGGGTTTTCCTGAAATTTTGCAAGGAGTTTCCTTGAATGAGTACACGAGAAAGTACGCCCCTCCCTTCGATGAATTCGAAGTTGATCGCTGTATTCTTCCACAAGCTGCATCTGTGTCCTTCCCATCTGTCCCAGGACCATCTCTCTTTCTAGTGATGTCTGGAAAAGGGACAATTGTGACAGGCTATTCTGAAGAAATTACATTTCAAGAGGGTGAAGTTCTGTTTGTTCCAGCATATATGGAGGTCAGTATAACAGCTGCAGCTATAGAATTACATATGTACAGAGCTGGGGTCAACAACAGATTCTTTGGAGACTTGTGA

Protein sequence

MDEEDDNKLKKCRKKRLSGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFLLNSGCCSLDPRECYAEFWIGTHKSGPSFVVFGRDNNAGAAFGSKPLSLKDWISVDPGAVLGDKVARKWGGDLPFLFKVLSIEKALSIQAHPDKDLARSLNQAQPSIYKDDNHKPEMALALTGFEALCGFISSKELKVVLSSVPEIVELVQCADAEKFSHESEQDGKEKVKQLFESIFSQIMSSNKGIVREAICKLKRRLSLEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYVGPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIVTGYSEEITFQEGEVLFVPAYMEVSITAAAIELHMYRAGVNNRFFGDL
Homology
BLAST of Bhi12G000008 vs. TAIR 10
Match: AT1G67070.1 (Mannose-6-phosphate isomerase, type I )

HSP 1 Score: 455.3 bits (1170), Expect = 5.7e-128
Identity = 230/429 (53.61%), Postives = 307/429 (71.56%), Query Frame = 0

Query: 24  KRLLRLKCCVQNYDWGVIGCNSQVARLFLLNSGCCSLDPRECYAEFWIGTHKSGPSFVVF 83
           + + RL+C V+NY+WG +G  S VARL   N+G   +D    YAEFW+GTH+SGPS V F
Sbjct: 20  EEIQRLRCFVKNYEWGKLGPESLVARLQEANTG-QRVDSEIPYAEFWMGTHESGPSHVEF 79

Query: 84  GRDNNAGAAFGSK-PLSLKDWISVDPGAVLGDKVARKWGGDLPFLFKVLSIEKALSIQAH 143
           G    +G     K  ++LK W+  +P  +LG KV  KWG DLPFLFKVLS+ KALSIQAH
Sbjct: 80  G----SGHGVSDKCMVTLKSWVLDNPN-LLGSKVVDKWGCDLPFLFKVLSVTKALSIQAH 139

Query: 144 PDKDLARSLNQAQPSIYKDDNHKPEMALALTGFEALCGFISSKELKVVLSSVPEIVELVQ 203
           P+K LA  L++  P +Y+D+NHKPE+ALA+T F+ALCGF++ KELK V+++VPEI ELV 
Sbjct: 140 PNKALAEKLHREDPLLYRDNNHKPEIALAVTPFQALCGFVTLKELKEVITNVPEITELVG 199

Query: 204 CADAEKFSHESEQDGKEKVKQLFESIFSQIMSSNKGIVREAICKLKRRLSLEKKKRQLSA 263
              A++  +  E D  E++K +   IF+Q+MS++    ++ + ++K RL LE K R+LS 
Sbjct: 200 SKAADQIFNVHEHDEDERIKSVVRLIFTQLMSASNNETKQVVSRMKNRLLLETKHRELSE 259

Query: 264 KEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYVGPNEPHAYISGECIECMATSDNV 323
           KE+L+L LE QY  D+G+++AFF NYV+L PGEALY+  NEPHAYISG+C+ECMA SDNV
Sbjct: 260 KEKLVLELEKQYTGDIGVISAFFFNYVKLNPGEALYLDANEPHAYISGDCVECMAASDNV 319

Query: 324 VRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTRKYAPPFDEFEVDRCILPQAAS 383
           VRAGLT K RDVQ L SML YK G+PEIL+G  L  Y  +Y PPFDEFEVD C LP+  S
Sbjct: 320 VRAGLTPKHRDVQTLCSMLTYKLGYPEILKGFPLTPYVTRYLPPFDEFEVDHCDLPRGKS 379

Query: 384 VSFPSVPGPSLFLVMSGKGTIVTGYSEEITFQEGEVLFVPAYMEVSITAAAIELHMYRAG 443
             FP+VPGPS++LV+ GKG + TG S ++    G+VLFVPA +E+ +T  +  + +YRAG
Sbjct: 380 TVFPAVPGPSVYLVIEGKGQLRTG-SSKVLVNRGDVLFVPADIEIHVTGESDVMKLYRAG 439

Query: 444 VNNRFFGDL 452
           V++RFF  L
Sbjct: 440 VSSRFFQTL 441

BLAST of Bhi12G000008 vs. TAIR 10
Match: AT3G02570.1 (Mannose-6-phosphate isomerase, type I )

HSP 1 Score: 452.2 bits (1162), Expect = 4.9e-127
Identity = 233/428 (54.44%), Postives = 313/428 (73.13%), Query Frame = 0

Query: 20  ADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFLLNSGCCSLDPRECYAEFWIGTHKSGPS 79
           ADRR RL RL+C V++YDWG IG +S V R++  NS    +DP   YAE W+GTH+SGPS
Sbjct: 15  ADRR-RLRRLRCSVKDYDWGKIGSDSLVYRVYAANSD-YEIDPTRPYAELWMGTHESGPS 74

Query: 80  FVVFGRDNNAGAAFGSKPLSLKDWISVDPGAVLGDKVARKWGGDLPFLFKVLSIEKALSI 139
           ++          A GS  ++L+ WI+ +P + LG++V  KWG DLPFLFKVLS+ + LSI
Sbjct: 75  YL--------EDADGSNGVTLRSWITENPKS-LGNRVLEKWGCDLPFLFKVLSVARPLSI 134

Query: 140 QAHPDKDLARSLNQAQPSIYKDDNHKPEMALALTGFEALCGFISSKELKVVLSSVPEIVE 199
           QAHPDK LA+ +++A P++YKDDNHKPEMALA T FEALCGFI  +ELK V+ ++PEI E
Sbjct: 135 QAHPDKKLAKKMHKAHPNLYKDDNHKPEMALAYTQFEALCGFIPLQELKSVIRAIPEIEE 194

Query: 200 LVQCADAEKFSHESEQDGKEKVKQLFESIFSQIMSSNKGIVREAICKLKRRLSLEKKKRQ 259
           LV   +A +    +E D +EKVK +  +IF+ +MS++    ++ + KLKRRL +E ++RQ
Sbjct: 195 LVGSEEANQVFCITEHD-EEKVKSVVRTIFTLLMSADADTTKKIVSKLKRRLHMESQERQ 254

Query: 260 LSAKEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYVGPNEPHAYISGECIECMATS 319
           L+ KE+L+L+LE QYP D+G+++AFF NYV+L PGEALY+G NEPHAY+ GEC+E MATS
Sbjct: 255 LTDKERLVLKLEKQYPNDIGVISAFFFNYVKLNPGEALYLGANEPHAYLFGECLEVMATS 314

Query: 320 DNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTRKYAPPFDEFEVDRCILPQ 379
           DNVVRAGLTSK  D+Q L SML+YK G+PEIL+G  +  Y  +Y PPF+EFEVD C LP 
Sbjct: 315 DNVVRAGLTSKPLDIQTLCSMLSYKLGYPEILKGTRIRPYITRYLPPFEEFEVDLCDLPS 374

Query: 380 AASVSFPSVPGPSLFLVMSGKGTIVTGYSEEITFQEGEVLFVPAYMEVSITAAAIELHMY 439
            AS  FPSVPGPSL LV+ G+G + T  S +     G+VLFVPA  E+ + +++ +L +Y
Sbjct: 375 GASTVFPSVPGPSLLLVLQGEGRMSTEASAD-GISMGDVLFVPADTEIHLRSSS-DLKLY 428

Query: 440 RAGVNNRF 448
           RAG+N+RF
Sbjct: 435 RAGINSRF 428

BLAST of Bhi12G000008 vs. ExPASy Swiss-Prot
Match: Q9FZH5 (Mannose-6-phosphate isomerase 2 OS=Arabidopsis thaliana OX=3702 GN=PMI2 PE=1 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 8.1e-127
Identity = 230/429 (53.61%), Postives = 307/429 (71.56%), Query Frame = 0

Query: 24  KRLLRLKCCVQNYDWGVIGCNSQVARLFLLNSGCCSLDPRECYAEFWIGTHKSGPSFVVF 83
           + + RL+C V+NY+WG +G  S VARL   N+G   +D    YAEFW+GTH+SGPS V F
Sbjct: 20  EEIQRLRCFVKNYEWGKLGPESLVARLQEANTG-QRVDSEIPYAEFWMGTHESGPSHVEF 79

Query: 84  GRDNNAGAAFGSK-PLSLKDWISVDPGAVLGDKVARKWGGDLPFLFKVLSIEKALSIQAH 143
           G    +G     K  ++LK W+  +P  +LG KV  KWG DLPFLFKVLS+ KALSIQAH
Sbjct: 80  G----SGHGVSDKCMVTLKSWVLDNPN-LLGSKVVDKWGCDLPFLFKVLSVTKALSIQAH 139

Query: 144 PDKDLARSLNQAQPSIYKDDNHKPEMALALTGFEALCGFISSKELKVVLSSVPEIVELVQ 203
           P+K LA  L++  P +Y+D+NHKPE+ALA+T F+ALCGF++ KELK V+++VPEI ELV 
Sbjct: 140 PNKALAEKLHREDPLLYRDNNHKPEIALAVTPFQALCGFVTLKELKEVITNVPEITELVG 199

Query: 204 CADAEKFSHESEQDGKEKVKQLFESIFSQIMSSNKGIVREAICKLKRRLSLEKKKRQLSA 263
              A++  +  E D  E++K +   IF+Q+MS++    ++ + ++K RL LE K R+LS 
Sbjct: 200 SKAADQIFNVHEHDEDERIKSVVRLIFTQLMSASNNETKQVVSRMKNRLLLETKHRELSE 259

Query: 264 KEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYVGPNEPHAYISGECIECMATSDNV 323
           KE+L+L LE QY  D+G+++AFF NYV+L PGEALY+  NEPHAYISG+C+ECMA SDNV
Sbjct: 260 KEKLVLELEKQYTGDIGVISAFFFNYVKLNPGEALYLDANEPHAYISGDCVECMAASDNV 319

Query: 324 VRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTRKYAPPFDEFEVDRCILPQAAS 383
           VRAGLT K RDVQ L SML YK G+PEIL+G  L  Y  +Y PPFDEFEVD C LP+  S
Sbjct: 320 VRAGLTPKHRDVQTLCSMLTYKLGYPEILKGFPLTPYVTRYLPPFDEFEVDHCDLPRGKS 379

Query: 384 VSFPSVPGPSLFLVMSGKGTIVTGYSEEITFQEGEVLFVPAYMEVSITAAAIELHMYRAG 443
             FP+VPGPS++LV+ GKG + TG S ++    G+VLFVPA +E+ +T  +  + +YRAG
Sbjct: 380 TVFPAVPGPSVYLVIEGKGQLRTG-SSKVLVNRGDVLFVPADIEIHVTGESDVMKLYRAG 439

Query: 444 VNNRFFGDL 452
           V++RFF  L
Sbjct: 440 VSSRFFQTL 441

BLAST of Bhi12G000008 vs. ExPASy Swiss-Prot
Match: Q9M884 (Mannose-6-phosphate isomerase 1 OS=Arabidopsis thaliana OX=3702 GN=PMI1 PE=1 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 6.8e-126
Identity = 233/428 (54.44%), Postives = 313/428 (73.13%), Query Frame = 0

Query: 20  ADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFLLNSGCCSLDPRECYAEFWIGTHKSGPS 79
           ADRR RL RL+C V++YDWG IG +S V R++  NS    +DP   YAE W+GTH+SGPS
Sbjct: 15  ADRR-RLRRLRCSVKDYDWGKIGSDSLVYRVYAANSD-YEIDPTRPYAELWMGTHESGPS 74

Query: 80  FVVFGRDNNAGAAFGSKPLSLKDWISVDPGAVLGDKVARKWGGDLPFLFKVLSIEKALSI 139
           ++          A GS  ++L+ WI+ +P + LG++V  KWG DLPFLFKVLS+ + LSI
Sbjct: 75  YL--------EDADGSNGVTLRSWITENPKS-LGNRVLEKWGCDLPFLFKVLSVARPLSI 134

Query: 140 QAHPDKDLARSLNQAQPSIYKDDNHKPEMALALTGFEALCGFISSKELKVVLSSVPEIVE 199
           QAHPDK LA+ +++A P++YKDDNHKPEMALA T FEALCGFI  +ELK V+ ++PEI E
Sbjct: 135 QAHPDKKLAKKMHKAHPNLYKDDNHKPEMALAYTQFEALCGFIPLQELKSVIRAIPEIEE 194

Query: 200 LVQCADAEKFSHESEQDGKEKVKQLFESIFSQIMSSNKGIVREAICKLKRRLSLEKKKRQ 259
           LV   +A +    +E D +EKVK +  +IF+ +MS++    ++ + KLKRRL +E ++RQ
Sbjct: 195 LVGSEEANQVFCITEHD-EEKVKSVVRTIFTLLMSADADTTKKIVSKLKRRLHMESQERQ 254

Query: 260 LSAKEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYVGPNEPHAYISGECIECMATS 319
           L+ KE+L+L+LE QYP D+G+++AFF NYV+L PGEALY+G NEPHAY+ GEC+E MATS
Sbjct: 255 LTDKERLVLKLEKQYPNDIGVISAFFFNYVKLNPGEALYLGANEPHAYLFGECLEVMATS 314

Query: 320 DNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYTRKYAPPFDEFEVDRCILPQ 379
           DNVVRAGLTSK  D+Q L SML+YK G+PEIL+G  +  Y  +Y PPF+EFEVD C LP 
Sbjct: 315 DNVVRAGLTSKPLDIQTLCSMLSYKLGYPEILKGTRIRPYITRYLPPFEEFEVDLCDLPS 374

Query: 380 AASVSFPSVPGPSLFLVMSGKGTIVTGYSEEITFQEGEVLFVPAYMEVSITAAAIELHMY 439
            AS  FPSVPGPSL LV+ G+G + T  S +     G+VLFVPA  E+ + +++ +L +Y
Sbjct: 375 GASTVFPSVPGPSLLLVLQGEGRMSTEASAD-GISMGDVLFVPADTEIHLRSSS-DLKLY 428

Query: 440 RAGVNNRF 448
           RAG+N+RF
Sbjct: 435 RAGINSRF 428

BLAST of Bhi12G000008 vs. ExPASy Swiss-Prot
Match: Q54PA0 (Probable mannose-6-phosphate isomerase OS=Dictyostelium discoideum OX=44689 GN=mpi PE=3 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 7.2e-75
Identity = 175/454 (38.55%), Postives = 255/454 (56.17%), Query Frame = 0

Query: 21  DRRKRLLRLKCCVQNYDWGVIGCNSQVARLFLLNSGCCSLDPREC--YAEFWIGTHKSGP 80
           +    ++ LKC  QNY+WG  G NS VA+L    +  CS   +E   YAE W+G H S P
Sbjct: 16  NNNNNIVLLKCVSQNYEWGKYGSNSTVAKLLKGYAKECSDIIKETIPYAELWMGDHVSAP 75

Query: 81  SFVVFGRDNNAGAAFGSKPLSLKDWI--------------SVDPGAVLGDKVARKWGGDL 140
           S V           + +K L L+++I              S    ++ G+ V +++G D 
Sbjct: 76  SKV----------EYKNKELKLREYIDTVQKEINEKSSTSSSSSSSIRGEIVEKRFGNDF 135

Query: 141 PFLFKVLSIEKALSIQAHPDKDLARSLNQAQPSIYKDDNHKPEMALALTGFEALCGFISS 200
           PFLFKVLSI  ALSIQAHPD  LA+ L +  P+IYKD  HKPE+A+A T FEALC F   
Sbjct: 136 PFLFKVLSIRTALSIQAHPDSQLAQVLFKKYPNIYKDPYHKPEIAIATTPFEALCSFRPL 195

Query: 201 KELKVVLSSVPEIVELVQCADAEKFSHESEQDGKEKVKQLFESIFSQIMSSNKGIVREAI 260
            E++  + ++PE           K S  +     +  K+  +SI + ++ ++  ++   +
Sbjct: 196 SEIQSFIDTIPEF----------KNSLPNNLIKLDDCKEYLKSIVTSLLKADGLLISNNL 255

Query: 261 CKLKRRLSLEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYVGPNEP 320
            +L  RL+ EK++ +    ++L+L+L  QYP DVG+  A+ LNY+ LKPGEAL++G  EP
Sbjct: 256 KELNNRLN-EKREEERDDLDRLVLKLYSQYPGDVGVFFAYILNYIVLKPGEALFLGAGEP 315

Query: 321 HAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEIL-----QGVSLNEY 380
           HAYISG+C+ECMA SDNVVRAGLT K +DV  L  ML Y+ G P+++       +SL  Y
Sbjct: 316 HAYISGDCVECMAPSDNVVRAGLTPKLKDVDTLGDMLTYRTGRPDLVVPQQRPDLSLTNY 375

Query: 381 TRKYAPPFDEFEVDRCILPQAA-SVSFPSVPGPSLFLVMSGKGTIVTGYSEEI--TFQEG 440
            R Y PP DEF+V+   L     +++  S  GPS+ LV SG  +I    ++        G
Sbjct: 376 -RCYQPPVDEFQVEYYHLDDCCKNINVYSSKGPSIVLVYSGNLSIENKSNQSTLNNLHTG 435

Query: 441 EVLFVPAYMEVSI--TAAAIELHMYRAGVNNRFF 449
            +LFVPA  +     + ++I + +Y A V+NR F
Sbjct: 436 SILFVPANTDYQFIQSDSSIPVSIYVASVSNRIF 447

BLAST of Bhi12G000008 vs. ExPASy Swiss-Prot
Match: Q9HFU4 (Mannose-6-phosphate isomerase OS=Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) OX=214684 GN=MAN1 PE=3 SV=2)

HSP 1 Score: 277.7 bits (709), Expect = 2.3e-73
Identity = 180/437 (41.19%), Postives = 239/437 (54.69%), Query Frame = 0

Query: 33  VQNYDWGVIGCNSQVARLFLLNSGCCSLDPRECYAEFWIGTHKSGPSFVVFGRDNNAGAA 92
           + +YDWG  G  S  A+L   +    S+D  + YAE W+GTH + PS +    DN     
Sbjct: 12  INSYDWGKKGSASLAAQLATTSIPDFSIDEDKAYAELWMGTHPNNPSRL---SDNTL--- 71

Query: 93  FGSKPLSLKDWISVDPGAVLGDKVARKW----GGDLPFLFKVLSIEKALSIQAHPDKDLA 152
                  L + +   P  ++G  V+ K+     G LPFLFKVLSI  ALSIQAHPDK LA
Sbjct: 72  -------LSEHLKSHP-ELIGSSVSSKFEDCKDGSLPFLFKVLSIGTALSIQAHPDKPLA 131

Query: 153 RSLNQAQPSIYKDDNHKPEMALALTGFEALCGFISSKELKVVLSSVPEIVELVQCADAEK 212
           + L   +P +YKD NHKPEMA+ALT F A   F+    L + L +VPE+ E V  +  E 
Sbjct: 132 KKLFDEKPDVYKDPNHKPEMAIALTPFLAFLNFLPLSVLLLHLLTVPELQEFVDSSLTES 191

Query: 213 FSHE----------------SEQDGKEKVKQLFESIFSQIMSSNKGIVREAICKLKRRLS 272
            +                  +E     + K + + IF+ +MS++K +V EAI KL +R  
Sbjct: 192 LASSLGLPTSQPPDTSLFKPTESPATAEQKDILKQIFAALMSADKKLVEEAISKLIKRYQ 251

Query: 273 LEKK-KRQLSAKEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYVGPNEPHAYISGE 332
            ++  K    +   L LRL  QYP DVG+L  F LN VELK GEA ++G NEPHAYI G+
Sbjct: 252 AKRDIKENEKSLVDLALRLNDQYPGDVGVLCVFLLNVVELKRGEAAFLGANEPHAYIEGD 311

Query: 333 CIECMATSDNVVRAGLTSKKRDVQALLSMLNYK--QGFPEILQGVSL---NEYTRKYAPP 392
            IECMATSDNVVRAGLT K RDV  L+SML Y+   G  ++LQ       ++ T+ Y PP
Sbjct: 312 IIECMATSDNVVRAGLTPKLRDVDTLVSMLTYEAAPGNKQLLQPTPFQKGDDTTKLYDPP 371

Query: 393 FDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIVTGYSEEITFQEGEVLFVPAYME 444
             EF V R  L +    S   V GPSL ++  G+G +  G ++   F  G+V+FV A  E
Sbjct: 372 IAEFSVLRTELSKGMKTSHRPVEGPSLCVITEGEGVVRNG-NDRSEFVRGDVIFVGAGKE 431

BLAST of Bhi12G000008 vs. ExPASy Swiss-Prot
Match: Q9GP38 (Probable mannose-6-phosphate isomerase OS=Echinococcus multilocularis OX=6211 GN=PMIH PE=2 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.8e-70
Identity = 175/453 (38.63%), Postives = 246/453 (54.30%), Query Frame = 0

Query: 26  LLRLKCCVQNYDWGVIGCNSQVARLFLLNSGCCSLDPRECYAEFWIGTHKSGPSFVVFGR 85
           +LRLKC  Q YDWG +G NS V +L + +           YAE W+GTH SG S      
Sbjct: 1   MLRLKCASQRYDWGKVGVNSIVYKLQVASENPDISHVDLPYAELWMGTHPSGSS------ 60

Query: 86  DNNAGAAFGSKPLSLKDWISVDPGAVLGDKVARKWGGDLPFLFKVLSIEKALSIQAHPDK 145
                  + +  ++L   I  +P A LG      +G  LPFLFKVLS+ KALSIQAHPDK
Sbjct: 61  -----CLWDNPHVTLAAHIQENP-ASLGKPSLIYFGRRLPFLFKVLSVAKALSIQAHPDK 120

Query: 146 DLARSLNQAQPSIYKDDNHKPEMALALTGFEALCGFISSKELKVVLSSVPEIVEL----- 205
            +A  L+  QP +YKD +HKPEMA+ALT FEAL GF    ++   + + PE+ EL     
Sbjct: 121 KMAVRLHAEQPDLYKDGSHKPEMAIALTDFEALLGFRPLNQILAFIQAFPELAELTTLEL 180

Query: 206 --------VQCADAEKFSHESEQDGKEKVKQLFESIFSQIMSSNKGIVREAICKLKRRLS 265
                   VQ    ++      +   EKV+   +S+ ++  + +K +    +      L 
Sbjct: 181 PSPEEKRDVQPPSIKQLYSNLMRSSPEKVESTIKSLLNRFTTGSKSVCDPPL----SILG 240

Query: 266 LEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYVGPNEPHAYISGEC 325
           ++ ++  + A   L LRL   +P DVG L+ FFLNY+ LK GEA+++  N PHAY+SG+C
Sbjct: 241 VDLQEEDVQALVDLFLRLTQAFPGDVGCLSIFFLNYIRLKSGEAIFLKANTPHAYLSGDC 300

Query: 326 IECMATSDNVVRAGLTSKKRDVQALLSMLNY-------KQGFPEIL---QGVSLNEYTRK 385
           +ECMA SDNVVRAGLT K +DV+ LL ML+Y       + G  + +   +G+S+    + 
Sbjct: 301 VECMANSDNVVRAGLTPKFKDVERLLEMLDYTPLTDSLRLGATQPIPTPEGISM----KS 360

Query: 386 YAPPFDEFEVDRCIL-PQAASVSFPSVPGPSLFLVMSGKGTIV---TG--YSEEITFQEG 445
           + PP  EF VD      ++   S PSVP  S+ L + G+GTI    TG   S+E  F  G
Sbjct: 361 FIPPVSEFAVDVIQFDAESRGFSLPSVPTASILLFLHGQGTITCPSTGEACSQETKFGPG 420

Query: 446 EVLFVPAYMEVSITAAAI---ELHMYRAGVNNR 447
            V FVPA M V++ +       LH +RA VN +
Sbjct: 421 FVYFVPADMIVNLVSKEDRKGSLHAFRAYVNRK 433

BLAST of Bhi12G000008 vs. NCBI nr
Match: XP_038875726.1 (mannose-6-phosphate isomerase 1-like [Benincasa hispida] >XP_038875727.1 mannose-6-phosphate isomerase 1-like [Benincasa hispida] >XP_038875728.1 mannose-6-phosphate isomerase 1-like [Benincasa hispida] >XP_038875729.1 mannose-6-phosphate isomerase 1-like [Benincasa hispida])

HSP 1 Score: 901.0 bits (2327), Expect = 4.2e-258
Identity = 451/451 (100.00%), Postives = 451/451 (100.00%), Query Frame = 0

Query: 1   MDEEDDNKLKKCRKKRLSGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFLLNSGCCSL 60
           MDEEDDNKLKKCRKKRLSGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFLLNSGCCSL
Sbjct: 1   MDEEDDNKLKKCRKKRLSGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFLLNSGCCSL 60

Query: 61  DPRECYAEFWIGTHKSGPSFVVFGRDNNAGAAFGSKPLSLKDWISVDPGAVLGDKVARKW 120
           DPRECYAEFWIGTHKSGPSFVVFGRDNNAGAAFGSKPLSLKDWISVDPGAVLGDKVARKW
Sbjct: 61  DPRECYAEFWIGTHKSGPSFVVFGRDNNAGAAFGSKPLSLKDWISVDPGAVLGDKVARKW 120

Query: 121 GGDLPFLFKVLSIEKALSIQAHPDKDLARSLNQAQPSIYKDDNHKPEMALALTGFEALCG 180
           GGDLPFLFKVLSIEKALSIQAHPDKDLARSLNQAQPSIYKDDNHKPEMALALTGFEALCG
Sbjct: 121 GGDLPFLFKVLSIEKALSIQAHPDKDLARSLNQAQPSIYKDDNHKPEMALALTGFEALCG 180

Query: 181 FISSKELKVVLSSVPEIVELVQCADAEKFSHESEQDGKEKVKQLFESIFSQIMSSNKGIV 240
           FISSKELKVVLSSVPEIVELVQCADAEKFSHESEQDGKEKVKQLFESIFSQIMSSNKGIV
Sbjct: 181 FISSKELKVVLSSVPEIVELVQCADAEKFSHESEQDGKEKVKQLFESIFSQIMSSNKGIV 240

Query: 241 REAICKLKRRLSLEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYVG 300
           REAICKLKRRLSLEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYVG
Sbjct: 241 REAICKLKRRLSLEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYVG 300

Query: 301 PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYT 360
           PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYT
Sbjct: 301 PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYT 360

Query: 361 RKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIVTGYSEEITFQEGEVLF 420
           RKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIVTGYSEEITFQEGEVLF
Sbjct: 361 RKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIVTGYSEEITFQEGEVLF 420

Query: 421 VPAYMEVSITAAAIELHMYRAGVNNRFFGDL 452
           VPAYMEVSITAAAIELHMYRAGVNNRFFGDL
Sbjct: 421 VPAYMEVSITAAAIELHMYRAGVNNRFFGDL 451

BLAST of Bhi12G000008 vs. NCBI nr
Match: XP_004143675.1 (mannose-6-phosphate isomerase 1 [Cucumis sativus] >XP_011654856.1 mannose-6-phosphate isomerase 1 [Cucumis sativus] >KGN50328.1 hypothetical protein Csa_000669 [Cucumis sativus])

HSP 1 Score: 849.0 bits (2192), Expect = 1.9e-242
Identity = 425/451 (94.24%), Postives = 436/451 (96.67%), Query Frame = 0

Query: 1   MDEEDDNKLKKCRKKRLSGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFLLNSGCCSL 60
           MDEEDD KL+KCRKKRL GADRRKRLLRLKCCVQNYDWGVIGCNSQVARLF LNSG C++
Sbjct: 1   MDEEDDKKLRKCRKKRLFGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFFLNSGRCNV 60

Query: 61  DPRECYAEFWIGTHKSGPSFVVFGRDNNAGAAFGSKPLSLKDWISVDPGAVLGDKVARKW 120
           DP ECYAEFWIGTHKSGPSFVV+GRDNNA AAFGSKPLSLKD ISVDP AVLGDKVARKW
Sbjct: 61  DPGECYAEFWIGTHKSGPSFVVYGRDNNAVAAFGSKPLSLKDCISVDPVAVLGDKVARKW 120

Query: 121 GGDLPFLFKVLSIEKALSIQAHPDKDLARSLNQAQPSIYKDDNHKPEMALALTGFEALCG 180
           GGDLPFLFKVLSIEKALSIQAHPDKDLARSLN+AQPSIYKDDNHKPEMALALT FEALCG
Sbjct: 121 GGDLPFLFKVLSIEKALSIQAHPDKDLARSLNEAQPSIYKDDNHKPEMALALTRFEALCG 180

Query: 181 FISSKELKVVLSSVPEIVELVQCADAEKFSHESEQDGKEKVKQLFESIFSQIMSSNKGIV 240
           FISSKELK VLSSVPEIVELVQCADAEKFSH+SEQDGKEKVKQLFESIFSQIMSSNKGIV
Sbjct: 181 FISSKELKAVLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIFSQIMSSNKGIV 240

Query: 241 REAICKLKRRLSLEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYVG 300
           REA+CKLKRRLSLEKKKRQLSAKEQLILRLE QYPADVGILAA+FLNYVELKPGEALYVG
Sbjct: 241 REAVCKLKRRLSLEKKKRQLSAKEQLILRLESQYPADVGILAAYFLNYVELKPGEALYVG 300

Query: 301 PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYT 360
           PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEIL+GVSLNEYT
Sbjct: 301 PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILRGVSLNEYT 360

Query: 361 RKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIVTGYSEEITFQEGEVLF 420
           RKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTI+TGYSEE T QEGEVLF
Sbjct: 361 RKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIITGYSEETTLQEGEVLF 420

Query: 421 VPAYMEVSITAAAIELHMYRAGVNNRFFGDL 452
           VPAYMEVSITA AIELHMYRAG+NNRFF DL
Sbjct: 421 VPAYMEVSITATAIELHMYRAGINNRFFRDL 451

BLAST of Bhi12G000008 vs. NCBI nr
Match: XP_023549674.1 (mannose-6-phosphate isomerase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 822.4 bits (2123), Expect = 1.9e-234
Identity = 414/452 (91.59%), Postives = 431/452 (95.35%), Query Frame = 0

Query: 1   MDEEDDNKLKKCRKKRLSGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFLLNSGCCSL 60
           M+EEDDN+LKK +KKRL GADRRKRLLRLKC VQNYDWGVIGC+SQVARLF LNSG  + 
Sbjct: 1   MNEEDDNRLKKRKKKRLLGADRRKRLLRLKCYVQNYDWGVIGCDSQVARLFSLNSGSGTP 60

Query: 61  DPRECYAEFWIGTHKSGPSFVVFGRD-NNAGAAFGSKPLSLKDWISVDPGAVLGDKVARK 120
           DPRECYAEFWIGTHKSGPSFVVFGRD NNA AA GS  +SLKDWISVDPGAVLGDKVARK
Sbjct: 61  DPRECYAEFWIGTHKSGPSFVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARK 120

Query: 121 WGGDLPFLFKVLSIEKALSIQAHPDKDLARSLNQAQPSIYKDDNHKPEMALALTGFEALC 180
           WGGDLPFLFKVLS+EKALSIQAHPDK+LAR+L++AQPSIYKDDNHKPEMALALTGFEALC
Sbjct: 121 WGGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALC 180

Query: 181 GFISSKELKVVLSSVPEIVELVQCADAEKFSHESEQDGKEKVKQLFESIFSQIMSSNKGI 240
           GFISSKELK VLSSVPEIVELVQC DAEKFSH+S QDGKEKVKQLFESIFSQIMSSNKGI
Sbjct: 181 GFISSKELKGVLSSVPEIVELVQCGDAEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKGI 240

Query: 241 VREAICKLKRRLSLEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYV 300
           VREAICKLKRRLS EKKKRQLSAKEQLILRLEGQYPADVGILA FFLNYV+LKPGEALYV
Sbjct: 241 VREAICKLKRRLSFEKKKRQLSAKEQLILRLEGQYPADVGILATFFLNYVKLKPGEALYV 300

Query: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY 360
           GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY
Sbjct: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY 360

Query: 361 TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIVTGYSEEITFQEGEVL 420
           TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLV+SGKGTI+TGYSEE T +EGEVL
Sbjct: 361 TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVISGKGTIITGYSEESTLEEGEVL 420

Query: 421 FVPAYMEVSITAAAIELHMYRAGVNNRFFGDL 452
           FVPAYMEVSIT+ A ELHMYRAGVNNRFFGDL
Sbjct: 421 FVPAYMEVSITSGATELHMYRAGVNNRFFGDL 452

BLAST of Bhi12G000008 vs. NCBI nr
Match: KAG6579210.1 (Mannose-6-phosphate isomerase 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016723.1 Mannose-6-phosphate isomerase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 822.0 bits (2122), Expect = 2.5e-234
Identity = 413/452 (91.37%), Postives = 431/452 (95.35%), Query Frame = 0

Query: 1   MDEEDDNKLKKCRKKRLSGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFLLNSGCCSL 60
           M+EEDDN+LKK +KKRL GADRRKRLLRLKC VQNYDWGVIGCNSQVARLF LNSG  + 
Sbjct: 1   MNEEDDNRLKKRKKKRLLGADRRKRLLRLKCYVQNYDWGVIGCNSQVARLFSLNSGSGTP 60

Query: 61  DPRECYAEFWIGTHKSGPSFVVFGRD-NNAGAAFGSKPLSLKDWISVDPGAVLGDKVARK 120
           DPRECYAEFWIGTHKSGPSFVVFGRD NNA AA GS  +SLKDWISVDPGAVLGDKVARK
Sbjct: 61  DPRECYAEFWIGTHKSGPSFVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARK 120

Query: 121 WGGDLPFLFKVLSIEKALSIQAHPDKDLARSLNQAQPSIYKDDNHKPEMALALTGFEALC 180
           WGGDLPFLFKVLS+EKALSIQAHPDK+LAR+L++AQPSIY+DDNHKPEMALALTGFEALC
Sbjct: 121 WGGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYEDDNHKPEMALALTGFEALC 180

Query: 181 GFISSKELKVVLSSVPEIVELVQCADAEKFSHESEQDGKEKVKQLFESIFSQIMSSNKGI 240
           GFISSKELK VLSSVPEIVELVQC D EKFSH+S QDGKEKVKQLFESIFSQIMSSNKGI
Sbjct: 181 GFISSKELKGVLSSVPEIVELVQCGDTEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKGI 240

Query: 241 VREAICKLKRRLSLEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYV 300
           VREAICKLKRRLS EKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYV+LKPGEALYV
Sbjct: 241 VREAICKLKRRLSFEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVKLKPGEALYV 300

Query: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY 360
           GPNEPHAYISGECIECMATSDNVVRAGLTSKKRD+QALLSMLNYKQGFPEILQGVSLNEY
Sbjct: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDIQALLSMLNYKQGFPEILQGVSLNEY 360

Query: 361 TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIVTGYSEEITFQEGEVL 420
           TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLV+SGKGTI+TGYSEE T QEGEVL
Sbjct: 361 TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVISGKGTIITGYSEESTLQEGEVL 420

Query: 421 FVPAYMEVSITAAAIELHMYRAGVNNRFFGDL 452
           FVPAYMEVSIT+ A ELH+YRAGVNNRFFGDL
Sbjct: 421 FVPAYMEVSITSGATELHVYRAGVNNRFFGDL 452

BLAST of Bhi12G000008 vs. NCBI nr
Match: XP_022938390.1 (mannose-6-phosphate isomerase 1-like [Cucurbita moschata])

HSP 1 Score: 819.7 bits (2116), Expect = 1.2e-233
Identity = 412/452 (91.15%), Postives = 430/452 (95.13%), Query Frame = 0

Query: 1   MDEEDDNKLKKCRKKRLSGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFLLNSGCCSL 60
           M+EEDDN+LKK +KKRL GADRRKRLLRLKC VQNYDWGVIGCNSQVARLF LNSG  + 
Sbjct: 1   MNEEDDNRLKKRKKKRLLGADRRKRLLRLKCYVQNYDWGVIGCNSQVARLFSLNSGSGTP 60

Query: 61  DPRECYAEFWIGTHKSGPSFVVFGRD-NNAGAAFGSKPLSLKDWISVDPGAVLGDKVARK 120
           DPRECYAEFWIGTHKSGPSFVVFGRD NNA AA GS  +SLKDWISVDPGAVLGDKVARK
Sbjct: 61  DPRECYAEFWIGTHKSGPSFVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARK 120

Query: 121 WGGDLPFLFKVLSIEKALSIQAHPDKDLARSLNQAQPSIYKDDNHKPEMALALTGFEALC 180
           WGGDLPFLFKVLS+EKALSIQAHPDK+LAR+L++AQPSIY+DDNHKPEMALALTGFEALC
Sbjct: 121 WGGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYEDDNHKPEMALALTGFEALC 180

Query: 181 GFISSKELKVVLSSVPEIVELVQCADAEKFSHESEQDGKEKVKQLFESIFSQIMSSNKGI 240
           GFISSKELK VLSSVPEIVELVQC D EKFSH+S QDGKEKVKQLFESIFSQIMSSNK I
Sbjct: 181 GFISSKELKGVLSSVPEIVELVQCGDTEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKAI 240

Query: 241 VREAICKLKRRLSLEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYV 300
           VREAICKLKRRLS EKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYV+LKPGEALYV
Sbjct: 241 VREAICKLKRRLSFEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVKLKPGEALYV 300

Query: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY 360
           GPNEPHAYISGECIECMATSDNVVRAGLTSKKRD+QALLSMLNYKQGFPEILQGVSLNEY
Sbjct: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDIQALLSMLNYKQGFPEILQGVSLNEY 360

Query: 361 TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIVTGYSEEITFQEGEVL 420
           TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLV+SGKGTI+TGYSEE T QEGEVL
Sbjct: 361 TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVISGKGTIITGYSEESTLQEGEVL 420

Query: 421 FVPAYMEVSITAAAIELHMYRAGVNNRFFGDL 452
           FVPAYMEVSIT+ A ELH+YRAGVNNRFFGDL
Sbjct: 421 FVPAYMEVSITSGATELHVYRAGVNNRFFGDL 452

BLAST of Bhi12G000008 vs. ExPASy TrEMBL
Match: A0A0A0KL16 (Mannose-6-phosphate isomerase OS=Cucumis sativus OX=3659 GN=Csa_5G167200 PE=3 SV=1)

HSP 1 Score: 849.0 bits (2192), Expect = 9.3e-243
Identity = 425/451 (94.24%), Postives = 436/451 (96.67%), Query Frame = 0

Query: 1   MDEEDDNKLKKCRKKRLSGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFLLNSGCCSL 60
           MDEEDD KL+KCRKKRL GADRRKRLLRLKCCVQNYDWGVIGCNSQVARLF LNSG C++
Sbjct: 1   MDEEDDKKLRKCRKKRLFGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFFLNSGRCNV 60

Query: 61  DPRECYAEFWIGTHKSGPSFVVFGRDNNAGAAFGSKPLSLKDWISVDPGAVLGDKVARKW 120
           DP ECYAEFWIGTHKSGPSFVV+GRDNNA AAFGSKPLSLKD ISVDP AVLGDKVARKW
Sbjct: 61  DPGECYAEFWIGTHKSGPSFVVYGRDNNAVAAFGSKPLSLKDCISVDPVAVLGDKVARKW 120

Query: 121 GGDLPFLFKVLSIEKALSIQAHPDKDLARSLNQAQPSIYKDDNHKPEMALALTGFEALCG 180
           GGDLPFLFKVLSIEKALSIQAHPDKDLARSLN+AQPSIYKDDNHKPEMALALT FEALCG
Sbjct: 121 GGDLPFLFKVLSIEKALSIQAHPDKDLARSLNEAQPSIYKDDNHKPEMALALTRFEALCG 180

Query: 181 FISSKELKVVLSSVPEIVELVQCADAEKFSHESEQDGKEKVKQLFESIFSQIMSSNKGIV 240
           FISSKELK VLSSVPEIVELVQCADAEKFSH+SEQDGKEKVKQLFESIFSQIMSSNKGIV
Sbjct: 181 FISSKELKAVLSSVPEIVELVQCADAEKFSHDSEQDGKEKVKQLFESIFSQIMSSNKGIV 240

Query: 241 REAICKLKRRLSLEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYVG 300
           REA+CKLKRRLSLEKKKRQLSAKEQLILRLE QYPADVGILAA+FLNYVELKPGEALYVG
Sbjct: 241 REAVCKLKRRLSLEKKKRQLSAKEQLILRLESQYPADVGILAAYFLNYVELKPGEALYVG 300

Query: 301 PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYT 360
           PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEIL+GVSLNEYT
Sbjct: 301 PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILRGVSLNEYT 360

Query: 361 RKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIVTGYSEEITFQEGEVLF 420
           RKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTI+TGYSEE T QEGEVLF
Sbjct: 361 RKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIITGYSEETTLQEGEVLF 420

Query: 421 VPAYMEVSITAAAIELHMYRAGVNNRFFGDL 452
           VPAYMEVSITA AIELHMYRAG+NNRFF DL
Sbjct: 421 VPAYMEVSITATAIELHMYRAGINNRFFRDL 451

BLAST of Bhi12G000008 vs. ExPASy TrEMBL
Match: A0A6J1FJN3 (Mannose-6-phosphate isomerase OS=Cucurbita moschata OX=3662 GN=LOC111444656 PE=3 SV=1)

HSP 1 Score: 819.7 bits (2116), Expect = 6.0e-234
Identity = 412/452 (91.15%), Postives = 430/452 (95.13%), Query Frame = 0

Query: 1   MDEEDDNKLKKCRKKRLSGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFLLNSGCCSL 60
           M+EEDDN+LKK +KKRL GADRRKRLLRLKC VQNYDWGVIGCNSQVARLF LNSG  + 
Sbjct: 1   MNEEDDNRLKKRKKKRLLGADRRKRLLRLKCYVQNYDWGVIGCNSQVARLFSLNSGSGTP 60

Query: 61  DPRECYAEFWIGTHKSGPSFVVFGRD-NNAGAAFGSKPLSLKDWISVDPGAVLGDKVARK 120
           DPRECYAEFWIGTHKSGPSFVVFGRD NNA AA GS  +SLKDWISVDPGAVLGDKVARK
Sbjct: 61  DPRECYAEFWIGTHKSGPSFVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARK 120

Query: 121 WGGDLPFLFKVLSIEKALSIQAHPDKDLARSLNQAQPSIYKDDNHKPEMALALTGFEALC 180
           WGGDLPFLFKVLS+EKALSIQAHPDK+LAR+L++AQPSIY+DDNHKPEMALALTGFEALC
Sbjct: 121 WGGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYEDDNHKPEMALALTGFEALC 180

Query: 181 GFISSKELKVVLSSVPEIVELVQCADAEKFSHESEQDGKEKVKQLFESIFSQIMSSNKGI 240
           GFISSKELK VLSSVPEIVELVQC D EKFSH+S QDGKEKVKQLFESIFSQIMSSNK I
Sbjct: 181 GFISSKELKGVLSSVPEIVELVQCGDTEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKAI 240

Query: 241 VREAICKLKRRLSLEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYV 300
           VREAICKLKRRLS EKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYV+LKPGEALYV
Sbjct: 241 VREAICKLKRRLSFEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVKLKPGEALYV 300

Query: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY 360
           GPNEPHAYISGECIECMATSDNVVRAGLTSKKRD+QALLSMLNYKQGFPEILQGVSLNEY
Sbjct: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDIQALLSMLNYKQGFPEILQGVSLNEY 360

Query: 361 TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIVTGYSEEITFQEGEVL 420
           TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLV+SGKGTI+TGYSEE T QEGEVL
Sbjct: 361 TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVISGKGTIITGYSEESTLQEGEVL 420

Query: 421 FVPAYMEVSITAAAIELHMYRAGVNNRFFGDL 452
           FVPAYMEVSIT+ A ELH+YRAGVNNRFFGDL
Sbjct: 421 FVPAYMEVSITSGATELHVYRAGVNNRFFGDL 452

BLAST of Bhi12G000008 vs. ExPASy TrEMBL
Match: A0A6J1E4R0 (Mannose-6-phosphate isomerase OS=Cucurbita moschata OX=3662 GN=LOC111429906 PE=3 SV=1)

HSP 1 Score: 818.9 bits (2114), Expect = 1.0e-233
Identity = 412/451 (91.35%), Postives = 431/451 (95.57%), Query Frame = 0

Query: 1   MDEEDDNKLKKCRKKRLSGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFLLNSGCCSL 60
           M EEDD +LKK +KKRL GADRRKRL+RLKCCVQNYDWGV+GCNSQVAR+FLLNSG  S 
Sbjct: 1   MAEEDDKRLKKRKKKRLIGADRRKRLVRLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSA 60

Query: 61  DPRECYAEFWIGTHKSGPSFVVFGRDNNAGAAFGSKPLSLKDWISVDPGAVLGDKVARKW 120
           DP ECYAEFWIGTHKSGPSFV FGRD NA AA GSKPLSLKDWI+VDPGAVLGDKVARKW
Sbjct: 61  DPDECYAEFWIGTHKSGPSFVFFGRDYNA-AAIGSKPLSLKDWITVDPGAVLGDKVARKW 120

Query: 121 GGDLPFLFKVLSIEKALSIQAHPDKDLARSLNQAQPSIYKDDNHKPEMALALTGFEALCG 180
           GGDLPFLFKVLS+EKALSIQAHPDK+LAR+L++AQPSIYKDDNHKPEMALALTGFEALCG
Sbjct: 121 GGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCG 180

Query: 181 FISSKELKVVLSSVPEIVELVQCADAEKFSHESEQDGKEKVKQLFESIFSQIMSSNKGIV 240
           FISSKELK VLSSVPEIVELVQ ADAEKFSH+SEQ+GKEKVK LFESIFSQIMSSNK +V
Sbjct: 181 FISSKELKGVLSSVPEIVELVQGADAEKFSHDSEQNGKEKVKSLFESIFSQIMSSNKAMV 240

Query: 241 REAICKLKRRLSLEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYVG 300
           REAICKLKRRLSLEKKKRQLSAKEQL+LRLE QYPADVGILAAFFLNYV+LKPGEALY+G
Sbjct: 241 REAICKLKRRLSLEKKKRQLSAKEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYLG 300

Query: 301 PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYT 360
           PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYT
Sbjct: 301 PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYT 360

Query: 361 RKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIVTGYSEEITFQEGEVLF 420
           RKYAPPFDEFEVDRCILP AASVSFPSVPGPSLFL+MSGKGTIVTGYSEEITFQEGEVLF
Sbjct: 361 RKYAPPFDEFEVDRCILPVAASVSFPSVPGPSLFLLMSGKGTIVTGYSEEITFQEGEVLF 420

Query: 421 VPAYMEVSITAAAIELHMYRAGVNNRFFGDL 452
           VPAYMEVS+TAAA ELHMYRAGVNNRFF DL
Sbjct: 421 VPAYMEVSVTAAATELHMYRAGVNNRFFKDL 450

BLAST of Bhi12G000008 vs. ExPASy TrEMBL
Match: A0A6J1K047 (Mannose-6-phosphate isomerase OS=Cucurbita maxima OX=3661 GN=LOC111489838 PE=3 SV=1)

HSP 1 Score: 815.1 bits (2104), Expect = 1.5e-232
Identity = 411/451 (91.13%), Postives = 429/451 (95.12%), Query Frame = 0

Query: 1   MDEEDDNKLKKCRKKRLSGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFLLNSGCCSL 60
           M+EEDDN+LKK +KKRL GADRRKRLLRLKC VQNYDWGVIGCNSQVARLF LNSG  + 
Sbjct: 1   MNEEDDNRLKKRKKKRLLGADRRKRLLRLKCYVQNYDWGVIGCNSQVARLFSLNSGSGTP 60

Query: 61  DPRECYAEFWIGTHKSGPSFVVFGRD-NNAGAAFGSKPLSLKDWISVDPGAVLGDKVARK 120
           DPRECYAEFWIGTHKSGPSFVVFGRD NNA AA GS  +SLKDWISVDPGAVLGDKVARK
Sbjct: 61  DPRECYAEFWIGTHKSGPSFVVFGRDYNNAAAALGSNAVSLKDWISVDPGAVLGDKVARK 120

Query: 121 WGGDLPFLFKVLSIEKALSIQAHPDKDLARSLNQAQPSIYKDDNHKPEMALALTGFEALC 180
           WGG+LPFLFKVLS+EKALSIQAHPDK+LAR+L++AQPSIYKDDNHKPEMALALTGFEALC
Sbjct: 121 WGGELPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALC 180

Query: 181 GFISSKELKVVLSSVPEIVELVQCADAEKFSHESEQDGKEKVKQLFESIFSQIMSSNKGI 240
           GFISSKELK VLSSVPEIVELVQC DAEKFSH+S QDGKEKVKQLFESIFSQIMSSNKGI
Sbjct: 181 GFISSKELKGVLSSVPEIVELVQCGDAEKFSHDSGQDGKEKVKQLFESIFSQIMSSNKGI 240

Query: 241 VREAICKLKRRLSLEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYV 300
           VR+AICKLKRRLS EKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYV+LKPGEALYV
Sbjct: 241 VRDAICKLKRRLSFEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVKLKPGEALYV 300

Query: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY 360
           GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY
Sbjct: 301 GPNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEY 360

Query: 361 TRKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIVTGYSEEITFQEGEVL 420
           TRKYAPPFDEFEVDRCILPQAASVSF SVPGPSLFLV+SGKGTI+TGYSEE T QEGEVL
Sbjct: 361 TRKYAPPFDEFEVDRCILPQAASVSFASVPGPSLFLVISGKGTIITGYSEESTLQEGEVL 420

Query: 421 FVPAYMEVSITAAAIELHMYRAGVNNRFFGD 451
           FVPAYMEVSIT+ A EL +YRAGVNNRFFGD
Sbjct: 421 FVPAYMEVSITSGATELRLYRAGVNNRFFGD 451

BLAST of Bhi12G000008 vs. ExPASy TrEMBL
Match: A0A6J1I443 (Mannose-6-phosphate isomerase OS=Cucurbita maxima OX=3661 GN=LOC111470445 PE=3 SV=1)

HSP 1 Score: 813.5 bits (2100), Expect = 4.3e-232
Identity = 409/451 (90.69%), Postives = 428/451 (94.90%), Query Frame = 0

Query: 1   MDEEDDNKLKKCRKKRLSGADRRKRLLRLKCCVQNYDWGVIGCNSQVARLFLLNSGCCSL 60
           M EEDD +LKK +KKRL GADRR+RLLRLKCCVQNYDWGV+GCNSQVAR+FLLNSG  S 
Sbjct: 1   MAEEDDKRLKKRKKKRLIGADRRRRLLRLKCCVQNYDWGVLGCNSQVARMFLLNSGSYSA 60

Query: 61  DPRECYAEFWIGTHKSGPSFVVFGRDNNAGAAFGSKPLSLKDWISVDPGAVLGDKVARKW 120
           DP ECYAEFWIGTHKSGPSFV FGRD NA AA GSKPLSLKDWI+VDPGAVLGDKVARKW
Sbjct: 61  DPDECYAEFWIGTHKSGPSFVFFGRDYNA-AAIGSKPLSLKDWITVDPGAVLGDKVARKW 120

Query: 121 GGDLPFLFKVLSIEKALSIQAHPDKDLARSLNQAQPSIYKDDNHKPEMALALTGFEALCG 180
           GGDLPFLFKVLS+EKALSIQAHPDK+LAR+L++AQPSIYKDDNHKPEMALALTGFEALCG
Sbjct: 121 GGDLPFLFKVLSVEKALSIQAHPDKELARALHEAQPSIYKDDNHKPEMALALTGFEALCG 180

Query: 181 FISSKELKVVLSSVPEIVELVQCADAEKFSHESEQDGKEKVKQLFESIFSQIMSSNKGIV 240
           FISSKELK VLSSVPEIVELVQ ADAEKFSH+SEQ+GKEKVK LFESIF QIMSSNK +V
Sbjct: 181 FISSKELKGVLSSVPEIVELVQGADAEKFSHDSEQNGKEKVKSLFESIFGQIMSSNKAMV 240

Query: 241 REAICKLKRRLSLEKKKRQLSAKEQLILRLEGQYPADVGILAAFFLNYVELKPGEALYVG 300
           REAICKLKRRLSLEKKKRQLS KEQL+LRLE QYPADVGILAAFFLNYV+LKPGEALY+G
Sbjct: 241 REAICKLKRRLSLEKKKRQLSTKEQLVLRLEAQYPADVGILAAFFLNYVKLKPGEALYLG 300

Query: 301 PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYT 360
           PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYT
Sbjct: 301 PNEPHAYISGECIECMATSDNVVRAGLTSKKRDVQALLSMLNYKQGFPEILQGVSLNEYT 360

Query: 361 RKYAPPFDEFEVDRCILPQAASVSFPSVPGPSLFLVMSGKGTIVTGYSEEITFQEGEVLF 420
           RKYAPPFDEFEVDRCILP AASVSFPSVPGPSLFL+MSGKGTIVTGYSEE TFQEGEVLF
Sbjct: 361 RKYAPPFDEFEVDRCILPVAASVSFPSVPGPSLFLLMSGKGTIVTGYSEESTFQEGEVLF 420

Query: 421 VPAYMEVSITAAAIELHMYRAGVNNRFFGDL 452
           VPAYMEVS+TAAA ELHMYRAGVNNRFF DL
Sbjct: 421 VPAYMEVSVTAAATELHMYRAGVNNRFFRDL 450

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT1G67070.15.7e-12853.61Mannose-6-phosphate isomerase, type I [more]
AT3G02570.14.9e-12754.44Mannose-6-phosphate isomerase, type I [more]
Match NameE-valueIdentityDescription
Q9FZH58.1e-12753.61Mannose-6-phosphate isomerase 2 OS=Arabidopsis thaliana OX=3702 GN=PMI2 PE=1 SV=... [more]
Q9M8846.8e-12654.44Mannose-6-phosphate isomerase 1 OS=Arabidopsis thaliana OX=3702 GN=PMI1 PE=1 SV=... [more]
Q54PA07.2e-7538.55Probable mannose-6-phosphate isomerase OS=Dictyostelium discoideum OX=44689 GN=m... [more]
Q9HFU42.3e-7341.19Mannose-6-phosphate isomerase OS=Cryptococcus neoformans var. neoformans serotyp... [more]
Q9GP381.8e-7038.63Probable mannose-6-phosphate isomerase OS=Echinococcus multilocularis OX=6211 GN... [more]
Match NameE-valueIdentityDescription
XP_038875726.14.2e-258100.00mannose-6-phosphate isomerase 1-like [Benincasa hispida] >XP_038875727.1 mannose... [more]
XP_004143675.11.9e-24294.24mannose-6-phosphate isomerase 1 [Cucumis sativus] >XP_011654856.1 mannose-6-phos... [more]
XP_023549674.11.9e-23491.59mannose-6-phosphate isomerase 1-like [Cucurbita pepo subsp. pepo][more]
KAG6579210.12.5e-23491.37Mannose-6-phosphate isomerase 2, partial [Cucurbita argyrosperma subsp. sororia]... [more]
XP_022938390.11.2e-23391.15mannose-6-phosphate isomerase 1-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A0A0KL169.3e-24394.24Mannose-6-phosphate isomerase OS=Cucumis sativus OX=3659 GN=Csa_5G167200 PE=3 SV... [more]
A0A6J1FJN36.0e-23491.15Mannose-6-phosphate isomerase OS=Cucurbita moschata OX=3662 GN=LOC111444656 PE=3... [more]
A0A6J1E4R01.0e-23391.35Mannose-6-phosphate isomerase OS=Cucurbita moschata OX=3662 GN=LOC111429906 PE=3... [more]
A0A6J1K0471.5e-23291.13Mannose-6-phosphate isomerase OS=Cucurbita maxima OX=3661 GN=LOC111489838 PE=3 S... [more]
A0A6J1I4434.3e-23290.69Mannose-6-phosphate isomerase OS=Cucurbita maxima OX=3661 GN=LOC111470445 PE=3 S... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 253..273
NoneNo IPR availableGENE3D1.10.441.10Phosphomannose Isomerase, domain 2coord: 183..349
e-value: 3.3E-131
score: 440.3
NoneNo IPR availablePIRSRPIRSR001480-1PIRSR001480-1coord: 34..442
e-value: 2.9E-122
score: 406.6
NoneNo IPR availablePIRSRPIRSR001480-2PIRSR001480-2coord: 34..442
e-value: 2.9E-122
score: 406.6
NoneNo IPR availablePANTHERPTHR10309:SF10MANNOSE-6-PHOSPHATE ISOMERASEcoord: 17..449
NoneNo IPR availableCDDcd02208cupin_RmlC-likecoord: 393..431
e-value: 1.02908E-4
score: 38.6178
NoneNo IPR availableCDDcd07011cupin_PMI_type_I_Ncoord: 29..343
e-value: 3.12757E-110
score: 323.347
IPR016305Mannose-6-phosphate isomerasePRINTSPR00714MAN6PISMRASEcoord: 159..182
score: 68.33
coord: 270..285
score: 38.12
coord: 65..80
score: 53.12
coord: 29..47
score: 46.32
coord: 286..305
score: 48.0
coord: 324..343
score: 52.0
coord: 305..324
score: 70.0
IPR016305Mannose-6-phosphate isomerasePIRSFPIRSF001480PMIcoord: 23..448
e-value: 5.8E-125
score: 415.7
IPR016305Mannose-6-phosphate isomerasePANTHERPTHR10309MANNOSE-6-PHOSPHATE ISOMERASEcoord: 17..449
IPR001250Mannose-6-phosphate isomerase, type IPFAMPF01238PMI_typeIcoord: 26..406
e-value: 9.9E-100
score: 334.3
IPR001250Mannose-6-phosphate isomerase, type ITIGRFAMTIGR00218TIGR00218coord: 26..432
e-value: 2.0E-73
score: 245.6
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 30..325
e-value: 3.3E-131
score: 440.3
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 26..441
e-value: 3.3E-131
score: 440.3
IPR018050Phosphomannose isomerase, type I, conserved sitePROSITEPS00965PMI_I_1coord: 159..167
IPR011051RmlC-like cupin domain superfamilySUPERFAMILY51182RmlC-like cupinscoord: 25..444

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi12M000008Bhi12M000008mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0000032 cell wall mannoprotein biosynthetic process
biological_process GO:0009298 GDP-mannose biosynthetic process
biological_process GO:0006486 protein glycosylation
cellular_component GO:0005829 cytosol
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004476 mannose-6-phosphate isomerase activity
molecular_function GO:0008270 zinc ion binding