Bhi12G000003 (gene) Wax gourd (B227) v1

Overview
NameBhi12G000003
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionPyridoxal 5'-phosphate synthase
Locationchr12: 17076 .. 19567 (-)
RNA-Seq ExpressionBhi12G000003
SyntenyBhi12G000003
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTAAATTCATTGTTCGATATCGGATATAAATTACTGAAACAGTTGAATTTCGTTTCTGAAAGCAGCAACTATTTCCCAGTGATGTATTGAACTGAAATGGGAAATAGAAAGCAGTAGGCGAATTAGCGGGCAATTAGGAAATGGGATCGGCAGCGGCGCCATGGAAGTCCCTTCTACTCGGTGCTTTGGAATCTAATGGCCGAATCAAGCATTCCAAATTCTTGCAGCTCGTACATTTCCCTCTCTCTATTTCTCTTCAAGTTCAAGTTTCCTTATTGCTTCTTCACAGTCGGTTTCTACGTCGAAATCGTTTCCAGGCAACGATAGGAATTAATGGAAGGCCCTCAAATCGCACAGTTGTTTTCAGAGGATTTGAAGAGGGCACCGATAGGATCCATATTTACACGGATTCTAGAACTCGCAAGGTTCTTCTTCTACGGGGAGGCTCTATATTTCCGTCTTATATAGTTCAATTCATGCGGCCATTGCTTTTTTTCTATGGTTTTTGATGCAGTGATTATATGGGCTTCTGGATTTTGATTGCACTTTGTAGTTTGTTTCTTTTTGTTGGTGTTTTCAATTACGTAATTCATATGTTTGGCAACAAGACAAGCGATAAAATAAGCTCAGGGGAATTGTTTGTGATGGATTAGGTCAGGGTTTTTTATATTAAGCATCTGAGTTTTGTCCTGCAAATGAATTTCAATTTTTGGTTGATTCTGCAGATTGAAGAGCTTAAGAATTGTCCATTTGCCGAGGTAAATGATTTGTTCATGCCATCTTTGTAGCTTATCATCTTTAGATTACTGCTGAAATATGTGCCAATTTGAGTCAAATTTTCCATTTCCAATGCTTTGTTCTTTAAGATTTGTTGGTATTTCACCGAGTCATGGGAGCAATTTCGTATCAGTGGGAGAATTGAAGTTGTTGATGCGTTATGTCTTGATGCTACAAAGTTTAAGGTTGTGGCATGGTCCTTTCTTCTTATGACTCGAGGCTTGTTCTACATTTTCAACAATTCACTTCATTCGATTGGGTATAGTCCTATACATTTTATCGTGGTCATATTGAATTCTGACAGCAAAGGGCAGAAGCCTGGTCCTCCATTTCTCCAAAGTCAAGATTGCAGTATTTGGGGCCTAGTCCAGGTCTTCCTTACCCTGGTGAACAACCAGTCAAGGAACCTTTTGTAGATTCTTATTCTGGTCCTGTTGATTCATTTTGTTTGCTGGTCTTCAATCCTGATCAGGTTTGTAATATTTTCTTTCTAGATTTGTATATGAAGCTTGCATTCTCCATCTTTTTACTTTCCCTTATGTTACCTTTTATCCTTTTTCGTTCTCAGGTCGATTATCTAAATCTGAAGAGTGAAGAAAGGAATTTGTTTAAAGCTATTCCAAGTTTTGGAGGGGAGAAATTATGGGAAACGGAGAGGATAAACCCTTAGCAATTGCACCCCGATTTGCATTTATATATACTTATAATAAGCTGCTATCTTCAAATCAACTGATGCAAGTTAAAAGTTACTCGTATGTGTTAAAAATAATCCGAAACCCTTTGTTTGTTAGTACTGATGATATTGGTTGGTTTCAATTTCTTGTGTCGCATATTGTTAGTTATGAAAACATCTTTAATTAATCCTTTCCTATGAGATTTTGTAGCTTTATTTGTTCAAACTCAGCTAGAGATGTGGCTGAGCAACAAAAAAGAGGGTGTCCAAACTCAAAAGAGGTATCCATTTCAACAAAGCATAATAGCAAATTCTGTTGTGTTGTTGAATATCTGTTTTGAATTTGTAACAGGAACTGACATTCGATGATGGAAGAGATCATGACAGAATTCTTACTTTTGTGATAAGGGAAGAGTCTTGTAAAGGGCCTGTTCTTGTGCTGTTTTGTGTGGAGTGCNNNNNNNNNNCATCCGCTAATCAGACTTTTTGTAATTTTTAGCTTAGTAAATTCTTTTAGATTGGAGTTCATTCTTATATCTTTTTGAAGACTCCTTTTGTAGGATTGTTACAATCTCTTGTAAATGAAAGTTTGGTTTCTTGTCTAACAAAAGATTAATGTCTGGTTAAAATTCAGTAGGGTATTTCAATATTAAAATCAGAAGATGCAACACAATCCATTATTAACCAGCGCTTATGCAAGGTTCAGGGTATTACAAAAGAAAGTTTGGTTTCAGTAGTATATTACAACCCAGCTTTAGCAGAGCAAAGCAATCTGTGCAAGGTTTATACCAAAAATGATTGCATTTATTGTCAGTTGTGCTTGATCTGTAATCAGAGAAACAACCAATAGCTGAATTGGAGGTTATAAAAAAAAAAAGTTATTGCTTCCAATTTTTGTACAATTCCTAGAGGCAATAAAAGATGTTCACTTAATGTGTGCCAAAACTAGGATTAATAAAACAAAACCCAGCAACTTGATTATCTTGTTCTGCTTGAATAATACACTACTCTTGTACTCTACTTGTCAAACTCCCAGATGAC

mRNA sequence

GTTAAATTCATTGTTCGATATCGGATATAAATTACTGAAACAGTTGAATTTCGTTTCTGAAAGCAGCAACTATTTCCCAGTGATGTATTGAACTGAAATGGGAAATAGAAAGCAGTAGGCGAATTAGCGGGCAATTAGGAAATGGGATCGGCAGCGGCGCCATGGAAGTCCCTTCTACTCGGTGCTTTGGAATCTAATGGCCGAATCAAGCATTCCAAATTCTTGCAGCTCGCAACGATAGGAATTAATGGAAGGCCCTCAAATCGCACAGTTGTTTTCAGAGGATTTGAAGAGGGCACCGATAGGATCCATATTTACACGGATTCTAGAACTCGCAAGATTGAAGAGCTTAAGAATTGTCCATTTGCCGAGATTTGTTGGTATTTCACCGAGTCATGGGAGCAATTTCGTATCAGTGGGAGAATTGAAGTTGTTGATGCGTTATGTCTTGATGCTACAAAGTTTAAGCAAAGGGCAGAAGCCTGGTCCTCCATTTCTCCAAAGTCAAGATTGCAGTATTTGGGGCCTAGTCCAGGTCTTCCTTACCCTGGTGAACAACCAGTCAAGGAACCTTTTGTAGATTCTTATTCTGGTCCTGTTGATTCATTTTGTTTGCTGGTCTTCAATCCTGATCAGGTCGATTATCTAAATCTGAAGAGTGAAGAAAGGAATTTGTTTAAAGCTATTCCAAGTTTTGGAGGGGAGAAATTATGGGAAACGGAGAGGATAAACCCTTAGCAATTGCACCCCGATTTGCATTTATATATACTTATAATAAGCTGCTATCTTCAAATCAACTGATGCAAGTTAAAAGTTACTCGTATGTGTTAAAAATAATCCGAAACCCTTTGTTTGTTAGTACTGATGATATTGGTTGGTTTCAATTTCTTGTGTCGCATATTGTTAGTTATGAAAACATCTTTAATTAATCCTTTCCTATGAGATTTTGTAGCTTTATTTGTTCAAACTCAGCTAGAGATGTGGCTGAGCAACAAAAAAGAGGGTGTCCAAACTCAAAAGAGGAACTGACATTCGATGATGGAAGAGATCATGACAGAATTCTTACTTTTGTGATAAGGGAAGAGTCTTGTAAAGGGCCTGTTCTTGTGCTGTTTTGTGTGGAGTGCNNNNNNNNNNCATCCGCTAATCAGACTTTTTGTAATTTTTAGCTTAGTAAATTCTTTTAGATTGGAGTTCATTCTTATATCTTTTTGAAGACTCCTTTTGTAGGATTGTTACAATCTCTTGTAAATGAAAGTTTGGTTTCTTGTCTAACAAAAGATTAATGTCTGGTTAAAATTCAGTAGGGTATTTCAATATTAAAATCAGAAGATGCAACACAATCCATTATTAACCAGCGCTTATGCAAGGTTCAGGGTATTACAAAAGAAAGTTTGGTTTCAGTAGTATATTACAACCCAGCTTTAGCAGAGCAAAGCAATCTGTGCAAGGTTTATACCAAAAATGATTGCATTTATTGTCAGTTGTGCTTGATCTGTAATCAGAGAAACAACCAATAGCTGAATTGGAGGTTATAAAAAAAAAAAGTTATTGCTTCCAATTTTTGTACAATTCCTAGAGGCAATAAAAGATGTTCACTTAATGTGTGCCAAAACTAGGATTAATAAAACAAAACCCAGCAACTTGATTATCTTGTTCTGCTTGAATAATACACTACTCTTGTACTCTACTTGTCAAACTCCCAGATGAC

Coding sequence (CDS)

ATGGGATCGGCAGCGGCGCCATGGAAGTCCCTTCTACTCGGTGCTTTGGAATCTAATGGCCGAATCAAGCATTCCAAATTCTTGCAGCTCGCAACGATAGGAATTAATGGAAGGCCCTCAAATCGCACAGTTGTTTTCAGAGGATTTGAAGAGGGCACCGATAGGATCCATATTTACACGGATTCTAGAACTCGCAAGATTGAAGAGCTTAAGAATTGTCCATTTGCCGAGATTTGTTGGTATTTCACCGAGTCATGGGAGCAATTTCGTATCAGTGGGAGAATTGAAGTTGTTGATGCGTTATGTCTTGATGCTACAAAGTTTAAGCAAAGGGCAGAAGCCTGGTCCTCCATTTCTCCAAAGTCAAGATTGCAGTATTTGGGGCCTAGTCCAGGTCTTCCTTACCCTGGTGAACAACCAGTCAAGGAACCTTTTGTAGATTCTTATTCTGGTCCTGTTGATTCATTTTGTTTGCTGGTCTTCAATCCTGATCAGGTCGATTATCTAAATCTGAAGAGTGAAGAAAGGAATTTGTTTAAAGCTATTCCAAGTTTTGGAGGGGAGAAATTATGGGAAACGGAGAGGATAAACCCTTAG

Protein sequence

MGSAAAPWKSLLLGALESNGRIKHSKFLQLATIGINGRPSNRTVVFRGFEEGTDRIHIYTDSRTRKIEELKNCPFAEICWYFTESWEQFRISGRIEVVDALCLDATKFKQRAEAWSSISPKSRLQYLGPSPGLPYPGEQPVKEPFVDSYSGPVDSFCLLVFNPDQVDYLNLKSEERNLFKAIPSFGGEKLWETERINP
Homology
[Close]
BLAST of Bhi12G000003 vs. TAIR 10
Match: AT2G46580.1 (Pyridoxamine 5'-phosphate oxidase family protein )

HSP 1 Score: 252.3 bits (643), Expect = 3.2e-67
Identity = 114/198 (57.58%), Postives = 152/198 (76.77%), Query Frame = 0 

Query: 1   MGSAAAPWKSLLLGALESNGRIKHSKFLQLATIGINGRPSNRTVVFRGFEEGTDRIHIYT 60
           MG+  APWK LL GA+E+N  + HS ++QLATIG+NGRPSNRTVVFRGFEE +DRI I T
Sbjct: 1   MGTHVAPWKQLLFGAIEANSHLSHSSYVQLATIGLNGRPSNRTVVFRGFEENSDRIQINT 60

Query: 61  DSRTRKIEELKNCPFAEICWYFTESWEQFRISGRIEVVDALCLDATKFKQRAEAWSSISP 120
           D R+RKIEELK+CPF+E+CWYF+++WEQFRI+GRIEV+DA   D TK +QR +AW + S 
Sbjct: 61  DLRSRKIEELKHCPFSEMCWYFSDTWEQFRINGRIEVIDASNPDQTKLQQREKAWFANSL 120

Query: 121 KSRLQYLGPSPGLPYPGEQPVKEPFVDSYSGPVDSFCLLVFNPDQVDYLNLKSEERNLFK 180
           +SRL Y+ P+PG P   EQ  ++  +D  SGPV  +CLL+  P++VDYLNLK+ +R  F 
Sbjct: 121 RSRLIYVCPTPGSPCNSEQSSQQVKLDPSSGPVPEYCLLLLEPEKVDYLNLKTNQRLFFS 180

Query: 181 AIPSFGGEKLWETERINP 199
           ++ +  GEK W +E++NP
Sbjct: 181 SMATGTGEKCWTSEKVNP 198

[Close]
BLAST of Bhi12G000003 vs. ExPASy Swiss-Prot
Match: Q9ZPY1 (Pyridoxine/pyridoxamine 5'-phosphate oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=PPOX2 PE=1 SV=2)

HSP 1 Score: 252.3 bits (643), Expect = 4.6e-66
Identity = 114/198 (57.58%), Postives = 152/198 (76.77%), Query Frame = 0 

Query: 1   MGSAAAPWKSLLLGALESNGRIKHSKFLQLATIGINGRPSNRTVVFRGFEEGTDRIHIYT 60
           MG+  APWK LL GA+E+N  + HS ++QLATIG+NGRPSNRTVVFRGFEE +DRI I T
Sbjct: 1   MGTHVAPWKQLLFGAIEANSHLSHSSYVQLATIGLNGRPSNRTVVFRGFEENSDRIQINT 60

Query: 61  DSRTRKIEELKNCPFAEICWYFTESWEQFRISGRIEVVDALCLDATKFKQRAEAWSSISP 120
           D R+RKIEELK+CPF+E+CWYF+++WEQFRI+GRIEV+DA   D TK +QR +AW + S 
Sbjct: 61  DLRSRKIEELKHCPFSEMCWYFSDTWEQFRINGRIEVIDASNPDQTKLQQREKAWFANSL 120

Query: 121 KSRLQYLGPSPGLPYPGEQPVKEPFVDSYSGPVDSFCLLVFNPDQVDYLNLKSEERNLFK 180
           +SRL Y+ P+PG P   EQ  ++  +D  SGPV  +CLL+  P++VDYLNLK+ +R  F 
Sbjct: 121 RSRLIYVCPTPGSPCNSEQSSQQVKLDPSSGPVPEYCLLLLEPEKVDYLNLKTNQRLFFS 180

Query: 181 AIPSFGGEKLWETERINP 199
           ++ +  GEK W +E++NP
Sbjct: 181 SMATGTGEKCWTSEKVNP 198

[Close]
BLAST of Bhi12G000003 vs. ExPASy Swiss-Prot
Match: P21159 (Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Myxococcus xanthus OX=34 GN=pdxH PE=1 SV=2)

HSP 1 Score: 57.0 bits (136), Expect = 2.8e-07
Identity = 34/94 (36.17%), Postives = 52/94 (55.32%), Query Frame = 0 

Query: 30  LATIGINGRPSNRTVVFRGFEEGTDRIHIYTDSRTRKIEELKNCPFAEICWYFTESWEQF 89
           +AT+G +GRPS R V+ + F+        YT+  +RK  E +  P+A +C+Y+    EQ 
Sbjct: 57  VATVGDDGRPSARVVLLKDFD--ARGFVFYTNHESRKGREARAHPYAALCFYWQPLNEQV 116

Query: 90  RISGRIEVVDALCLDATKFKQRAE-----AWSSI 119
           R+ GR+E V     DA  F+ RA      AW+S+
Sbjct: 117 RVEGRVERVTDAEADA-YFQSRARGSQVGAWASL 147

[Close]
BLAST of Bhi12G000003 vs. ExPASy Swiss-Prot
Match: Q1DCS1 (Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Myxococcus xanthus (strain DK1622) OX=246197 GN=pdxH PE=3 SV=2)

HSP 1 Score: 57.0 bits (136), Expect = 2.8e-07
Identity = 34/94 (36.17%), Postives = 52/94 (55.32%), Query Frame = 0 

Query: 30  LATIGINGRPSNRTVVFRGFEEGTDRIHIYTDSRTRKIEELKNCPFAEICWYFTESWEQF 89
           +AT+G +GRPS R V+ + F+        YT+  +RK  E +  P+A +C+Y+    EQ 
Sbjct: 43  VATVGDDGRPSARVVLLKDFD--ARGFVFYTNHESRKGREARAHPYAALCFYWQPLNEQV 102

Query: 90  RISGRIEVVDALCLDATKFKQRAE-----AWSSI 119
           R+ GR+E V     DA  F+ RA      AW+S+
Sbjct: 103 RVEGRVERVTDAEADA-YFQSRARGSQVGAWASL 133

[Close]
BLAST of Bhi12G000003 vs. ExPASy Swiss-Prot
Match: P53210 (Uncharacterized protein YGR017W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YGR017W PE=1 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.8e-07
Identity = 39/112 (34.82%), Postives = 56/112 (50.00%), Query Frame = 0 

Query: 1   MGSAAAPWKSLLLGALESNGRIKHSKFLQLATIG-INGRPSNRTVVFRGF---EEGTDRI 60
           M    APW  + + + ++N     S   Q AT+  +  +P  RTVVFR F   ++ T+ +
Sbjct: 1   MSHQMAPWIPMFIQSCKNNTEPFVS--FQFATVDELTNKPRCRTVVFRDFLFHDKRTNVL 60

Query: 61  HIYTDSRTRKIEE------------LKNC--PFAEICWYFTESWEQFRISGR 95
              TD R+ KI E             K C  PF E C+YF E+WEQ+R SG+
Sbjct: 61  TFNTDMRSSKITESFITPNSNNSSDSKRCETPFFEACFYFPETWEQYRFSGQ 110

[Close]
BLAST of Bhi12G000003 vs. ExPASy Swiss-Prot
Match: B2SY78 (Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Paraburkholderia phytofirmans (strain DSM 17436 / LMG 22146 / PsJN) OX=398527 GN=pdxH PE=3 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 6.3e-07
Identity = 42/140 (30.00%), Postives = 64/140 (45.71%), Query Frame = 0 

Query: 19  NGRIKHSKFLQLATIGINGRPSNRTVVFRGFEEGTDRIHIYTDSRTRKIEELKNCPFAEI 78
           + ++     + LAT+   GRPS R V+ +G +E       +T+  +RK  EL   P+A +
Sbjct: 38  DAKLPEPNTMTLATVDSRGRPSARIVLIKGVDE--RGFVFFTNYESRKGRELAANPYASL 97

Query: 79  CWYFTESWEQFRISGRIEVVDALCLDA----TKFKQRAEAWSS-----ISPKSRLQYLGP 138
            +Y+ E   Q R+ GRI    A   DA         R  AW+S     I  +S+L+    
Sbjct: 98  LFYWIELERQVRVEGRIVKTSAEESDAYFASRPLGSRIGAWASNQSQLIESRSQLETRER 157

Query: 139 SPGLPYPGEQPVKEPFVDSY 150
              L Y G+QP + P    Y
Sbjct: 158 EISLQY-GDQPPRPPHWGGY 174

[Close]
BLAST of Bhi12G000003 vs. NCBI nr
Match: XP_038874699.1 (pyridoxine/pyridoxamine 5'-phosphate oxidase 2-like isoform X2 [Benincasa hispida])

HSP 1 Score: 416.0 bits (1068), Expect = 1.8e-112
Identity = 198/198 (100.00%), Postives = 198/198 (100.00%), Query Frame = 0 

Query: 1   MGSAAAPWKSLLLGALESNGRIKHSKFLQLATIGINGRPSNRTVVFRGFEEGTDRIHIYT 60
           MGSAAAPWKSLLLGALESNGRIKHSKFLQLATIGINGRPSNRTVVFRGFEEGTDRIHIYT
Sbjct: 1   MGSAAAPWKSLLLGALESNGRIKHSKFLQLATIGINGRPSNRTVVFRGFEEGTDRIHIYT 60

Query: 61  DSRTRKIEELKNCPFAEICWYFTESWEQFRISGRIEVVDALCLDATKFKQRAEAWSSISP 120
           DSRTRKIEELKNCPFAEICWYFTESWEQFRISGRIEVVDALCLDATKFKQRAEAWSSISP
Sbjct: 61  DSRTRKIEELKNCPFAEICWYFTESWEQFRISGRIEVVDALCLDATKFKQRAEAWSSISP 120

Query: 121 KSRLQYLGPSPGLPYPGEQPVKEPFVDSYSGPVDSFCLLVFNPDQVDYLNLKSEERNLFK 180
           KSRLQYLGPSPGLPYPGEQPVKEPFVDSYSGPVDSFCLLVFNPDQVDYLNLKSEERNLFK
Sbjct: 121 KSRLQYLGPSPGLPYPGEQPVKEPFVDSYSGPVDSFCLLVFNPDQVDYLNLKSEERNLFK 180

Query: 181 AIPSFGGEKLWETERINP 199
           AIPSFGGEKLWETERINP
Sbjct: 181 AIPSFGGEKLWETERINP 198

[Close]
BLAST of Bhi12G000003 vs. NCBI nr
Match: XP_038899249.1 (pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X1 [Benincasa hispida])

HSP 1 Score: 409.8 bits (1052), Expect = 1.3e-110
Identity = 195/198 (98.48%), Postives = 196/198 (98.99%), Query Frame = 0 

Query: 1   MGSAAAPWKSLLLGALESNGRIKHSKFLQLATIGINGRPSNRTVVFRGFEEGTDRIHIYT 60
           MGSAAAPWKSLLLGALESNGRIKHSKFLQLATIGINGRPSNRTVVFRGFEEGTD+IHIYT
Sbjct: 1   MGSAAAPWKSLLLGALESNGRIKHSKFLQLATIGINGRPSNRTVVFRGFEEGTDKIHIYT 60

Query: 61  DSRTRKIEELKNCPFAEICWYFTESWEQFRISGRIEVVDALCLDATKFKQRAEAWSSISP 120
           DSRTRKIEELKNCPFAEICWYFTESWEQFRISGRIEVVDA CLDATKFKQRAEAWSSISP
Sbjct: 61  DSRTRKIEELKNCPFAEICWYFTESWEQFRISGRIEVVDASCLDATKFKQRAEAWSSISP 120

Query: 121 KSRLQYLGPSPGLPYPGEQPVKEPFVDSYSGPVDSFCLLVFNPDQVDYLNLKSEERNLFK 180
           KSRLQYLGPSPGLPYPGEQPVKEPFVDSYSGPVDSFCLLVFNPDQVDYLNLKSEERNLFK
Sbjct: 121 KSRLQYLGPSPGLPYPGEQPVKEPFVDSYSGPVDSFCLLVFNPDQVDYLNLKSEERNLFK 180

Query: 181 AIPSFGGEKLWETERINP 199
           AIPSFGGEKLWET RINP
Sbjct: 181 AIPSFGGEKLWETGRINP 198

[Close]
BLAST of Bhi12G000003 vs. NCBI nr
Match: XP_038874698.1 (pyridoxine/pyridoxamine 5'-phosphate oxidase 2-like isoform X1 [Benincasa hispida])

HSP 1 Score: 400.6 bits (1028), Expect = 7.9e-108
Identity = 198/227 (87.22%), Postives = 198/227 (87.22%), Query Frame = 0 

Query: 1   MGSAAAPWKSLLLGALESNGRIKHSKFLQL-----------------------------A 60
           MGSAAAPWKSLLLGALESNGRIKHSKFLQL                             A
Sbjct: 1   MGSAAAPWKSLLLGALESNGRIKHSKFLQLVHFPLSISLQVQVSLLLLHSRFLRRNRFQA 60

Query: 61  TIGINGRPSNRTVVFRGFEEGTDRIHIYTDSRTRKIEELKNCPFAEICWYFTESWEQFRI 120
           TIGINGRPSNRTVVFRGFEEGTDRIHIYTDSRTRKIEELKNCPFAEICWYFTESWEQFRI
Sbjct: 61  TIGINGRPSNRTVVFRGFEEGTDRIHIYTDSRTRKIEELKNCPFAEICWYFTESWEQFRI 120

Query: 121 SGRIEVVDALCLDATKFKQRAEAWSSISPKSRLQYLGPSPGLPYPGEQPVKEPFVDSYSG 180
           SGRIEVVDALCLDATKFKQRAEAWSSISPKSRLQYLGPSPGLPYPGEQPVKEPFVDSYSG
Sbjct: 121 SGRIEVVDALCLDATKFKQRAEAWSSISPKSRLQYLGPSPGLPYPGEQPVKEPFVDSYSG 180

Query: 181 PVDSFCLLVFNPDQVDYLNLKSEERNLFKAIPSFGGEKLWETERINP 199
           PVDSFCLLVFNPDQVDYLNLKSEERNLFKAIPSFGGEKLWETERINP
Sbjct: 181 PVDSFCLLVFNPDQVDYLNLKSEERNLFKAIPSFGGEKLWETERINP 227

[Close]
BLAST of Bhi12G000003 vs. NCBI nr
Match: KAA0048877.1 (pyridoxine/pyridoxamine 5'-phosphate oxidase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 381.7 bits (979), Expect = 3.8e-102
Identity = 179/198 (90.40%), Postives = 190/198 (95.96%), Query Frame = 0 

Query: 1   MGSAAAPWKSLLLGALESNGRIKHSKFLQLATIGINGRPSNRTVVFRGFEEGTDRIHIYT 60
           MGSA APWKSLLL ALESNG+IKHSKFLQLATIG NGRPSNRTVVFR FEEGTDRIHIYT
Sbjct: 1   MGSAVAPWKSLLLRALESNGQIKHSKFLQLATIGTNGRPSNRTVVFREFEEGTDRIHIYT 60

Query: 61  DSRTRKIEELKNCPFAEICWYFTESWEQFRISGRIEVVDALCLDATKFKQRAEAWSSISP 120
           DSR+RKIEELKNCPFAEICWYFT++WEQFRISGR+EVVDA CLDATKFKQRAEAWSSISP
Sbjct: 61  DSRSRKIEELKNCPFAEICWYFTDTWEQFRISGRMEVVDASCLDATKFKQRAEAWSSISP 120

Query: 121 KSRLQYLGPSPGLPYPGEQPVKEPFVDSYSGPVDSFCLLVFNPDQVDYLNLKSEERNLFK 180
           KSR+QYLGPSPGLP+  EQP KEPF+DSYSGPVD+FCLLVF+PDQVDYLNLKSEERNLF+
Sbjct: 121 KSRMQYLGPSPGLPHLAEQPAKEPFLDSYSGPVDAFCLLVFDPDQVDYLNLKSEERNLFR 180

Query: 181 AIPSFGGEKLWETERINP 199
           AIPSFGGEKLWETERINP
Sbjct: 181 AIPSFGGEKLWETERINP 198

[Close]
BLAST of Bhi12G000003 vs. NCBI nr
Match: XP_008437871.1 (PREDICTED: pyridoxine/pyridoxamine 5'-phosphate oxidase 2 [Cucumis melo] >XP_016898998.1 PREDICTED: pyridoxine/pyridoxamine 5'-phosphate oxidase 2 [Cucumis melo] >TYK20828.1 pyridoxine/pyridoxamine 5'-phosphate oxidase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 381.3 bits (978), Expect = 5.0e-102
Identity = 178/198 (89.90%), Postives = 190/198 (95.96%), Query Frame = 0 

Query: 1   MGSAAAPWKSLLLGALESNGRIKHSKFLQLATIGINGRPSNRTVVFRGFEEGTDRIHIYT 60
           MGSA APWKSLLL ALESNG+IKHSKFLQLATIG NGRPSNRTVVFR FEEGTDRIHIYT
Sbjct: 1   MGSAVAPWKSLLLRALESNGQIKHSKFLQLATIGTNGRPSNRTVVFREFEEGTDRIHIYT 60

Query: 61  DSRTRKIEELKNCPFAEICWYFTESWEQFRISGRIEVVDALCLDATKFKQRAEAWSSISP 120
           DSR+RKIEELKNCPFAE+CWYFT++WEQFRISGR+EVVDA CLDATKFKQRAEAWSSISP
Sbjct: 61  DSRSRKIEELKNCPFAEVCWYFTDTWEQFRISGRMEVVDASCLDATKFKQRAEAWSSISP 120

Query: 121 KSRLQYLGPSPGLPYPGEQPVKEPFVDSYSGPVDSFCLLVFNPDQVDYLNLKSEERNLFK 180
           KSR+QYLGPSPGLP+  EQP KEPF+DSYSGPVD+FCLLVF+PDQVDYLNLKSEERNLF+
Sbjct: 121 KSRMQYLGPSPGLPHLAEQPAKEPFLDSYSGPVDAFCLLVFDPDQVDYLNLKSEERNLFR 180

Query: 181 AIPSFGGEKLWETERINP 199
           AIPSFGGEKLWETERINP
Sbjct: 181 AIPSFGGEKLWETERINP 198

[Close]
BLAST of Bhi12G000003 vs. ExPASy TrEMBL
Match: A0A5A7U3K2 (Pyridoxal 5'-phosphate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G00920 PE=4 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 1.8e-102
Identity = 179/198 (90.40%), Postives = 190/198 (95.96%), Query Frame = 0 

Query: 1   MGSAAAPWKSLLLGALESNGRIKHSKFLQLATIGINGRPSNRTVVFRGFEEGTDRIHIYT 60
           MGSA APWKSLLL ALESNG+IKHSKFLQLATIG NGRPSNRTVVFR FEEGTDRIHIYT
Sbjct: 1   MGSAVAPWKSLLLRALESNGQIKHSKFLQLATIGTNGRPSNRTVVFREFEEGTDRIHIYT 60

Query: 61  DSRTRKIEELKNCPFAEICWYFTESWEQFRISGRIEVVDALCLDATKFKQRAEAWSSISP 120
           DSR+RKIEELKNCPFAEICWYFT++WEQFRISGR+EVVDA CLDATKFKQRAEAWSSISP
Sbjct: 61  DSRSRKIEELKNCPFAEICWYFTDTWEQFRISGRMEVVDASCLDATKFKQRAEAWSSISP 120

Query: 121 KSRLQYLGPSPGLPYPGEQPVKEPFVDSYSGPVDSFCLLVFNPDQVDYLNLKSEERNLFK 180
           KSR+QYLGPSPGLP+  EQP KEPF+DSYSGPVD+FCLLVF+PDQVDYLNLKSEERNLF+
Sbjct: 121 KSRMQYLGPSPGLPHLAEQPAKEPFLDSYSGPVDAFCLLVFDPDQVDYLNLKSEERNLFR 180

Query: 181 AIPSFGGEKLWETERINP 199
           AIPSFGGEKLWETERINP
Sbjct: 181 AIPSFGGEKLWETERINP 198

[Close]
BLAST of Bhi12G000003 vs. ExPASy TrEMBL
Match: A0A1S4DSQ7 (Pyridoxal 5'-phosphate synthase OS=Cucumis melo OX=3656 GN=LOC103483173 PE=4 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 2.4e-102
Identity = 178/198 (89.90%), Postives = 190/198 (95.96%), Query Frame = 0 

Query: 1   MGSAAAPWKSLLLGALESNGRIKHSKFLQLATIGINGRPSNRTVVFRGFEEGTDRIHIYT 60
           MGSA APWKSLLL ALESNG+IKHSKFLQLATIG NGRPSNRTVVFR FEEGTDRIHIYT
Sbjct: 1   MGSAVAPWKSLLLRALESNGQIKHSKFLQLATIGTNGRPSNRTVVFREFEEGTDRIHIYT 60

Query: 61  DSRTRKIEELKNCPFAEICWYFTESWEQFRISGRIEVVDALCLDATKFKQRAEAWSSISP 120
           DSR+RKIEELKNCPFAE+CWYFT++WEQFRISGR+EVVDA CLDATKFKQRAEAWSSISP
Sbjct: 61  DSRSRKIEELKNCPFAEVCWYFTDTWEQFRISGRMEVVDASCLDATKFKQRAEAWSSISP 120

Query: 121 KSRLQYLGPSPGLPYPGEQPVKEPFVDSYSGPVDSFCLLVFNPDQVDYLNLKSEERNLFK 180
           KSR+QYLGPSPGLP+  EQP KEPF+DSYSGPVD+FCLLVF+PDQVDYLNLKSEERNLF+
Sbjct: 121 KSRMQYLGPSPGLPHLAEQPAKEPFLDSYSGPVDAFCLLVFDPDQVDYLNLKSEERNLFR 180

Query: 181 AIPSFGGEKLWETERINP 199
           AIPSFGGEKLWETERINP
Sbjct: 181 AIPSFGGEKLWETERINP 198

[Close]
BLAST of Bhi12G000003 vs. ExPASy TrEMBL
Match: A0A5D3DBA0 (Pyridoxal 5'-phosphate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold291G00900 PE=4 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 2.4e-102
Identity = 178/198 (89.90%), Postives = 190/198 (95.96%), Query Frame = 0 

Query: 1   MGSAAAPWKSLLLGALESNGRIKHSKFLQLATIGINGRPSNRTVVFRGFEEGTDRIHIYT 60
           MGSA APWKSLLL ALESNG+IKHSKFLQLATIG NGRPSNRTVVFR FEEGTDRIHIYT
Sbjct: 1   MGSAVAPWKSLLLRALESNGQIKHSKFLQLATIGTNGRPSNRTVVFREFEEGTDRIHIYT 60

Query: 61  DSRTRKIEELKNCPFAEICWYFTESWEQFRISGRIEVVDALCLDATKFKQRAEAWSSISP 120
           DSR+RKIEELKNCPFAE+CWYFT++WEQFRISGR+EVVDA CLDATKFKQRAEAWSSISP
Sbjct: 61  DSRSRKIEELKNCPFAEVCWYFTDTWEQFRISGRMEVVDASCLDATKFKQRAEAWSSISP 120

Query: 121 KSRLQYLGPSPGLPYPGEQPVKEPFVDSYSGPVDSFCLLVFNPDQVDYLNLKSEERNLFK 180
           KSR+QYLGPSPGLP+  EQP KEPF+DSYSGPVD+FCLLVF+PDQVDYLNLKSEERNLF+
Sbjct: 121 KSRMQYLGPSPGLPHLAEQPAKEPFLDSYSGPVDAFCLLVFDPDQVDYLNLKSEERNLFR 180

Query: 181 AIPSFGGEKLWETERINP 199
           AIPSFGGEKLWETERINP
Sbjct: 181 AIPSFGGEKLWETERINP 198

[Close]
BLAST of Bhi12G000003 vs. ExPASy TrEMBL
Match: A0A0A0L745 (Pyridoxal 5'-phosphate synthase OS=Cucumis sativus OX=3659 GN=Csa_3G119450 PE=4 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 4.2e-99
Identity = 174/197 (88.32%), Postives = 184/197 (93.40%), Query Frame = 0 

Query: 1   MGSAAAPWKSLLLGALESNGRIKHSKFLQLATIGINGRPSNRTVVFRGFEEGTDRIHIYT 60
           MGSA APWKSLLL ALESNG  KHSKFLQLATIG NGRPSNRTVVFRGFEEGTDRIHIYT
Sbjct: 1   MGSAVAPWKSLLLSALESNGHFKHSKFLQLATIGTNGRPSNRTVVFRGFEEGTDRIHIYT 60

Query: 61  DSRTRKIEELKNCPFAEICWYFTESWEQFRISGRIEVVDALCLDATKFKQRAEAWSSISP 120
           DSR RKIEELKNCPFAEICWYFTESWEQFRISGR+EVVDA C+DATKFKQRAEAWSSISP
Sbjct: 61  DSRNRKIEELKNCPFAEICWYFTESWEQFRISGRMEVVDASCVDATKFKQRAEAWSSISP 120

Query: 121 KSRLQYLGPSPGLPYPGEQPVKEPFVDSYSGPVDSFCLLVFNPDQVDYLNLKSEERNLFK 180
           KSR+QYLGPSPGLP+  EQP KEPF+DS SGPVD+FCLLVF+PDQVDYLN++S+ERN FK
Sbjct: 121 KSRMQYLGPSPGLPHLAEQPAKEPFLDSCSGPVDTFCLLVFDPDQVDYLNVRSDERNSFK 180

Query: 181 AIPSFGGEKLWETERIN 198
           AI SFGGEKLWETERIN
Sbjct: 181 AITSFGGEKLWETERIN 197

[Close]
BLAST of Bhi12G000003 vs. ExPASy TrEMBL
Match: A0A6J1EFU0 (Pyridoxal 5'-phosphate synthase OS=Cucurbita moschata OX=3662 GN=LOC111432127 PE=4 SV=1)

HSP 1 Score: 352.4 bits (903), Expect = 1.2e-93
Identity = 169/198 (85.35%), Postives = 177/198 (89.39%), Query Frame = 0 

Query: 1   MGSAAAPWKSLLLGALESNGRIKHSKFLQLATIGINGRPSNRTVVFRGFEEGTDRIHIYT 60
           MGSAAAPWKSLLL ALESN +IKHS FL LATIG NGRPSNRTVVFRGFEEGTDRIHIYT
Sbjct: 24  MGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRTVVFRGFEEGTDRIHIYT 83

Query: 61  DSRTRKIEELKNCPFAEICWYFTESWEQFRISGRIEVVDALCLDATKFKQRAEAWSSISP 120
           DSR+RKIEELK CPFAEI WYFTESWEQFRISGRIEVVDA CLDATKFK+RAEAWSSISP
Sbjct: 84  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 143

Query: 121 KSRLQYLGPSPGLPYPGEQPVKEPFVDSYSGPVDSFCLLVFNPDQVDYLNLKSEERNLFK 180
           KSR+QYLGPSP LPY  EQP KEP +DS SGPVD+FCLLV +PDQVDYLNLKS ER LFK
Sbjct: 144 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 203

Query: 181 AIPSFGGEKLWETERINP 199
           AIP  G E LWE+ERINP
Sbjct: 204 AIPCVGRETLWESERINP 221

The following BLAST results are available for this feature:
BLAST of Bhi12G000003 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G46580.13.2e-6757.58Pyridoxamine 5'-phosphate oxidase family protein [more]
BLAST of Bhi12G000003 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9ZPY14.6e-6657.58Pyridoxine/pyridoxamine 5'-phosphate oxidase 2 OS=Arabidopsis thaliana OX=3702 G... [more]
P211592.8e-0736.17Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Myxococcus xanthus OX=34 GN=pdxH... [more]
Q1DCS12.8e-0736.17Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Myxococcus xanthus (strain DK162... [more]
P532102.8e-0734.82Uncharacterized protein YGR017W OS=Saccharomyces cerevisiae (strain ATCC 204508 ... [more]
B2SY786.3e-0730.00Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Paraburkholderia phytofirmans (s... [more]
BLAST of Bhi12G000003 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038874699.11.8e-112100.00pyridoxine/pyridoxamine 5'-phosphate oxidase 2-like isoform X2 [Benincasa hispid... [more]
XP_038899249.11.3e-11098.48pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X1 [Benincasa hispida][more]
XP_038874698.17.9e-10887.22pyridoxine/pyridoxamine 5'-phosphate oxidase 2-like isoform X1 [Benincasa hispid... [more]
KAA0048877.13.8e-10290.40pyridoxine/pyridoxamine 5'-phosphate oxidase 2 [Cucumis melo var. makuwa][more]
XP_008437871.15.0e-10289.90PREDICTED: pyridoxine/pyridoxamine 5'-phosphate oxidase 2 [Cucumis melo] >XP_016... [more]
BLAST of Bhi12G000003 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5A7U3K21.8e-10290.40Pyridoxal 5'-phosphate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A1S4DSQ72.4e-10289.90Pyridoxal 5'-phosphate synthase OS=Cucumis melo OX=3656 GN=LOC103483173 PE=4 SV=... [more]
A0A5D3DBA02.4e-10289.90Pyridoxal 5'-phosphate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A0A0L7454.2e-9988.32Pyridoxal 5'-phosphate synthase OS=Cucumis sativus OX=3659 GN=Csa_3G119450 PE=4 ... [more]
A0A6J1EFU01.2e-9385.35Pyridoxal 5'-phosphate synthase OS=Cucurbita moschata OX=3662 GN=LOC111432127 PE... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012349FMN-binding split barrelGENE3D2.30.110.10coord: 3..198
e-value: 1.8E-53
score: 183.3
IPR024015Pyridoxamine 5'-phosphate oxidase, probable FMN-dependent, Alr4036 familyTIGRFAMTIGR04026TIGR04026coord: 6..198
e-value: 6.6E-50
score: 167.6
IPR024624Pyridoxamine 5'-phosphate oxidase, Alr4036 family, FMN-binding domainPFAMPF12766Pyridox_oxase_2coord: 6..98
e-value: 3.0E-27
score: 95.2
NoneNo IPR availablePANTHERPTHR10851:SF3PYRIDOXINE/PYRIDOXAMINE 5'-PHOSPHATE OXIDASE 2coord: 2..198
NoneNo IPR availableSUPERFAMILY50475FMN-binding split barrelcoord: 6..198
IPR000659Pyridoxamine 5'-phosphate oxidasePANTHERPTHR10851PYRIDOXINE-5-PHOSPHATE OXIDASEcoord: 2..198

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi12M000003Bhi12M000003mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042823 pyridoxal phosphate biosynthetic process
biological_process GO:0008615 pyridoxine biosynthetic process
molecular_function GO:0010181 FMN binding
molecular_function GO:0004733 pyridoxamine-phosphate oxidase activity