Bhi11G002482 (gene) Wax gourd (B227) v1

Overview


Name	Bhi11G002482
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	ribonuclease MC-like
Location	chr11: 80870369 .. 80871516 (-)
RNA-Seq Expression	Bhi11G002482
Synteny	Bhi11G002482

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCAATGTCCCATATTGAGGTCCATTAACCAAAAGTATAATAAATCATGGGCAAGAGAGAAATTATGTTGGTTTTGAGCATATTGTTGCTATTGGTAGGAAGCTCTAAAGGGTTGAATGAATTTGACTACTACCAAGTTATTTTCCAATGGCAACCAGCTGCTTGTAGTAGCGCAACTCTAAAATCTCCATGTGTCAAACGTCCAGAAAATAGGTTCACTATCCATGGTGTTTGGCCTAGCCTCACATCTGGAAATGCTACAAAATGCTCTGGCACTCCCCCCTATGATGGTAATAAGGTAATTAATTATGGAAGATTATTGTATAATTGAATCTCGGCTCCGTTTTGAATTATAGTAGCTTTTAAAGTAGTAGTAGACATTTATATTTAAAAAAATCAGCTGTCACTAAAGTAGATTAGTAAGTTTAAATTAAATTGGTTGTGACATGATCATAATGACTAAGTTAGACAAAGTTAGATTAAAAAGTTCAAACAAAGCCTTAACTAATTACTCATTTGATTTGGTAGATATACGCAATTCGGAGTAGGCTGGATCAAGATTGGCCAAGTGTGGTTTATGGAACAAATCCAACATTGTGGAGCCATGAGTGGAAAACCCACGGAACTTGTTCAAATTACTATTTGGACCAATACAACTATTTTAACTTTGGACTTAGTGTATTTGGTAAGTACAATATGATATCCATTCTCAGAAAATATGGTATCAATCCAGATGGAAATGATTACTCCATAGACTACATTGAAAGTGCAATTTATAATGCAACGGTCAAATGGCCAGCCCTTCGCTGCAACAAAAACAAGTCGTCTGGTAAAAAACAATTATATGAAATTGCTTTGTGCTTCACAAAGGTTAATCGAGCTCTTCAAGATTGTCCCTACAACGCTGTTACATGTGGCAATAAATTCTCCTGGAATTCATGAAGAGGCTGTGTGCATTATGTGGAGGAACTACTACCCCTACAAGCTAATTAAGGGGCTTCTTAATAAATGGCTTGAAAAGTACTATGTATCTTCATGTGGTTTTCTTTGTAAGTGTTTTGTAACAATGTATGCCTTCATATGTTATGAATGGAAGAATTACTTCAATCTTATGAGACTTTTGAATAGTTTTATCTAACTATATTCCT

mRNA sequence

ATCAATGTCCCATATTGAGGTCCATTAACCAAAAGTATAATAAATCATGGGCAAGAGAGAAATTATGTTGGTTTTGAGCATATTGTTGCTATTGGTAGGAAGCTCTAAAGGGTTGAATGAATTTGACTACTACCAAGTTATTTTCCAATGGCAACCAGCTGCTTGTAGTAGCGCAACTCTAAAATCTCCATGTGTCAAACGTCCAGAAAATAGGTTCACTATCCATGGTGTTTGGCCTAGCCTCACATCTGGAAATGCTACAAAATGCTCTGGCACTCCCCCCTATGATGGTAATAAGATATACGCAATTCGGAGTAGGCTGGATCAAGATTGGCCAAGTGTGGTTTATGGAACAAATCCAACATTGTGGAGCCATGAGTGGAAAACCCACGGAACTTGTTCAAATTACTATTTGGACCAATACAACTATTTTAACTTTGGACTTAGTGTATTTGGTAAGTACAATATGATATCCATTCTCAGAAAATATGGTATCAATCCAGATGGAAATGATTACTCCATAGACTACATTGAAAGTGCAATTTATAATGCAACGGTCAAATGGCCAGCCCTTCGCTGCAACAAAAACAAGTCGTCTGGTAAAAAACAATTATATGAAATTGCTTTGTGCTTCACAAAGGTTAATCGAGCTCTTCAAGATTGTCCCTACAACGCTGTTACATGTGGCAATAAATTCTCCTGGAATTCATGAAGAGGCTGTGTGCATTATGTGGAGGAACTACTACCCCTACAAGCTAATTAAGGGGCTTCTTAATAAATGGCTTGAAAAGTACTATGTATCTTCATGTGGTTTTCTTTGTAAGTGTTTTGTAACAATGTATGCCTTCATATGTTATGAATGGAAGAATTACTTCAATCTTATGAGACTTTTGAATAGTTTTATCTAACTATATTCCT

Coding sequence (CDS)

ATGGGCAAGAGAGAAATTATGTTGGTTTTGAGCATATTGTTGCTATTGGTAGGAAGCTCTAAAGGGTTGAATGAATTTGACTACTACCAAGTTATTTTCCAATGGCAACCAGCTGCTTGTAGTAGCGCAACTCTAAAATCTCCATGTGTCAAACGTCCAGAAAATAGGTTCACTATCCATGGTGTTTGGCCTAGCCTCACATCTGGAAATGCTACAAAATGCTCTGGCACTCCCCCCTATGATGGTAATAAGATATACGCAATTCGGAGTAGGCTGGATCAAGATTGGCCAAGTGTGGTTTATGGAACAAATCCAACATTGTGGAGCCATGAGTGGAAAACCCACGGAACTTGTTCAAATTACTATTTGGACCAATACAACTATTTTAACTTTGGACTTAGTGTATTTGGTAAGTACAATATGATATCCATTCTCAGAAAATATGGTATCAATCCAGATGGAAATGATTACTCCATAGACTACATTGAAAGTGCAATTTATAATGCAACGGTCAAATGGCCAGCCCTTCGCTGCAACAAAAACAAGTCGTCTGGTAAAAAACAATTATATGAAATTGCTTTGTGCTTCACAAAGGTTAATCGAGCTCTTCAAGATTGTCCCTACAACGCTGTTACATGTGGCAATAAATTCTCCTGGAATTCATGA

Protein sequence

MGKREIMLVLSILLLLVGSSKGLNEFDYYQVIFQWQPAACSSATLKSPCVKRPENRFTIHGVWPSLTSGNATKCSGTPPYDGNKIYAIRSRLDQDWPSVVYGTNPTLWSHEWKTHGTCSNYYLDQYNYFNFGLSVFGKYNMISILRKYGINPDGNDYSIDYIESAIYNATVKWPALRCNKNKSSGKKQLYEIALCFTKVNRALQDCPYNAVTCGNKFSWNS

Homology

[Close]

BLAST of Bhi11G002482 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 135.6 bits (340), Expect = 4.9e-32
Identity = 68/208 (32.69%), Postives = 110/208 (52.88%), Query Frame = 0


Query: 6   IMLVLSILLLLVGSSKGLNEFDYYQVIFQWQPAACSSATLKSPCVK---RPENRFTIHGV 65
           ++ +L IL  +  +S    +FD++  + QW  + C   T K  C     +P   F IHG+
Sbjct: 10  LISLLVILPSVFSASSSSEDFDFFYFVQQWPGSYCD--TQKKCCYPNSGKPAADFGIHGL 69

Query: 66  WPSLTSGN-ATKCSGTPPYDGNKIYAIRSRLDQDWPSVV--YGTNPTLWSHEWKTHGTCS 125
           WP+   G   + C  + P+D + I  + + + + WP++    G+    W HEW+ HGTCS
Sbjct: 70  WPNYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCS 129

Query: 126 NYYLDQYNYFNFGLSVFGKYNMISILRKYGINPDGNDYSIDYIESAIYNATVKWPALRCN 185
              +DQ+ YF   L++  K N++  L K GINPDG  YS++ I  +I  +    P + CN
Sbjct: 130 ESVIDQHEYFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECN 189

Query: 186 KNKSSGKKQLYEIALCFTKVNRALQDCP 208
           ++  SG  QLY++ LC  +    L +CP
Sbjct: 190 RD-GSGNSQLYQVYLCVDRSGSGLIECP 214

[Close]

BLAST of Bhi11G002482 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 126.3 bits (316), Expect = 3.0e-29
Identity = 70/213 (32.86%), Postives = 110/213 (51.64%), Query Frame = 0


Query: 1   MGKREIMLVLSILLLLVG--SSKGLNEFDYYQVIFQWQPAAC-SSATLKSPCVKRPENRF 60
           MG +  + VL  LL+  G   S+   +FD++  + QW  A C +S     P   +P   F
Sbjct: 1   MGAKGCVNVLLKLLVFQGLFVSRPQEDFDFFYFVLQWPGAYCDTSRACCYPTSGKPAADF 60

Query: 61  TIHGVWPSLTSGN-ATKCSGTPPYDGNKIYAIRSRLDQDWPSVVYGTNP--TLWSHEWKT 120
            IHG+WP+   G+  + C     +D ++I  + S L ++WP++   +N     W HEW+ 
Sbjct: 61  GIHGLWPNYNGGSWPSNCDPDSQFDRSQISDLVSSLKKNWPTLSCPSNEGFNFWEHEWEK 120

Query: 121 HGTCSNYYLDQYNYFNFGLSVFGKYNMISILRKYGINPDGNDYSIDYIESAIYNATVKWP 180
           HGTCS   +DQ+ YF   L +  K N++ IL+  GINPD   Y++D I +AI +     P
Sbjct: 121 HGTCSESVMDQHEYFENALKLKQKANLLQILKNSGINPDDGFYNLDKITNAIKDGIGFTP 180

Query: 181 ALRCNKNKSSGKKQLYEIALCFTKVNRALQDCP 208
            + CNK+      QL++I +C         +CP
Sbjct: 181 GIECNKDPER-NAQLHQIYICVDTSGTEFIECP 212

[Close]

BLAST of Bhi11G002482 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 122.1 bits (305), Expect = 5.6e-28
Identity = 64/187 (34.22%), Postives = 97/187 (51.87%), Query Frame = 0


Query: 25  EFDYYQVIFQWQPAACSSA-TLKSPCVKRPENRFTIHGVWPSL-TSGNATKCSGTPPYDG 84
           +FD++  + QW  A C S  +   P   +P   F IHG+WP+  T G    C+    +D 
Sbjct: 21  DFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPNYKTGGWPQNCNPDSRFDD 80

Query: 85  NKIYAIRSRLDQDWPSVVYGTNPTL--WSHEWKTHGTCSNYYLDQYNYFNFGLSVFGKYN 144
            ++  + S L ++WP++   +N  +  W+HEW+ HGTC+   LDQ++YF  GL +  K N
Sbjct: 81  LRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESELDQHDYFEAGLKLKQKAN 140

Query: 145 MISILRKYGINPDGNDYSIDYIESAIYNATVKWPALRCNKNKSSGKKQLYEIALCFTKVN 204
           ++  L   GI PD   Y +  IE+ I       P + CNK+ SS   QLY+I LC     
Sbjct: 141 LLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNKD-SSHNSQLYQIYLCVDTSA 200

Query: 205 RALQDCP 208
               +CP
Sbjct: 201 SKFINCP 206

[Close]

BLAST of Bhi11G002482 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 96.3 bits (238), Expect = 3.3e-20
Identity = 62/211 (29.38%), Postives = 100/211 (47.39%), Query Frame = 0


Query: 6   IMLVLSILLLLVGSSKGLN----EFDYYQVIFQWQPAACSS---ATLKSPCVKRPE--NR 65
           ++LV  I     G    LN    EFDY+ +  QW    C        K+ C +  +   +
Sbjct: 8   LLLVACIAGAFAGDVIELNRSQREFDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQ 67

Query: 66  FTIHGVWPSLTSGNATKCSGTPPYDGNKIYAIRSRLDQDWPSVVYGT-------NPTLWS 125
           FTIHG+WP    G+   C     +   +I  +   L++ WPS+  G+         + W 
Sbjct: 68  FTIHGLWPDYNDGSWPSCCYRSDFKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWG 127

Query: 126 HEWKTHGTCSN-YYLDQYNYFNFGLSVFGKYNMISILRKYG-INPDGNDYSIDYIESAIY 185
           HEW+ HGTCS+  + D+YNYF   L+++ K+N+  +L + G +  +   Y +  I +AI 
Sbjct: 128 HEWEKHGTCSSPVFHDEYNYFLTTLNLYLKHNVTDVLYQAGYVASNSEKYPLGGIVTAIQ 187

Query: 186 NATVKWPALRCNKNKSSGKKQLYEIALCFTK 199
           NA    P + C ++       + EI +CF K
Sbjct: 188 NAFHITPEVVCKRD------AIDEIRICFYK 212

[Close]

BLAST of Bhi11G002482 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 6.9e-31
Identity = 68/208 (32.69%), Postives = 110/208 (52.88%), Query Frame = 0


Query: 6   IMLVLSILLLLVGSSKGLNEFDYYQVIFQWQPAACSSATLKSPCVK---RPENRFTIHGV 65
           ++ +L IL  +  +S    +FD++  + QW  + C   T K  C     +P   F IHG+
Sbjct: 10  LISLLVILPSVFSASSSSEDFDFFYFVQQWPGSYCD--TQKKCCYPNSGKPAADFGIHGL 69

Query: 66  WPSLTSGN-ATKCSGTPPYDGNKIYAIRSRLDQDWPSVV--YGTNPTLWSHEWKTHGTCS 125
           WP+   G   + C  + P+D + I  + + + + WP++    G+    W HEW+ HGTCS
Sbjct: 70  WPNYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCS 129

Query: 126 NYYLDQYNYFNFGLSVFGKYNMISILRKYGINPDGNDYSIDYIESAIYNATVKWPALRCN 185
              +DQ+ YF   L++  K N++  L K GINPDG  YS++ I  +I  +    P + CN
Sbjct: 130 ESVIDQHEYFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECN 189

Query: 186 KNKSSGKKQLYEIALCFTKVNRALQDCP 208
           ++  SG  QLY++ LC  +    L +CP
Sbjct: 190 RD-GSGNSQLYQVYLCVDRSGSGLIECP 214

[Close]

BLAST of Bhi11G002482 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 2.5e-28
Identity = 65/192 (33.85%), Postives = 93/192 (48.44%), Query Frame = 0


Query: 26  FDYYQVIFQWQPAACSSATLKSPCVKRPENRFTIHGVWPSLTSGNATKCSGTPPYDGNKI 85
           FD +  + QW PA CS     S C       FTIHG+WP  +  + T C    P+D  KI
Sbjct: 1   FDSFWFVQQWPPAVCSFQKSGS-CPGSGLRTFTIHGLWPQGSGTSLTNCPQGSPFDITKI 60

Query: 86  YAIRSRLDQDWPSVVYGTNPTLWSHEWKTHGTCSNYYLDQYNYFNFGLSVFGKYNMISIL 145
             ++S+L+  WP+V+   N   WSHEW  HGTCS    +Q  YF   + +   Y++I  L
Sbjct: 61  SHLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSESTFNQAAYFKLAVDMRNNYDIIGAL 120

Query: 146 RKYGINPDGNDYSIDYIESAIYNATVKWPALRCNKNKSSGKKQLYEIALCFTKVNRALQD 205
           R +   P+G   S   I+  +     K+P LRC  +  +    L ++  CF +    L D
Sbjct: 121 RPHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVQVVACFAQDGSTLID 180

Query: 206 CPYNAVTCGNKF 218
           C  +  TCG  F
Sbjct: 181 CTRD--TCGANF 189

[Close]

BLAST of Bhi11G002482 vs. ExPASy Swiss-Prot
Match: P42815 (Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 7.9e-27
Identity = 64/187 (34.22%), Postives = 97/187 (51.87%), Query Frame = 0


Query: 25  EFDYYQVIFQWQPAACSSA-TLKSPCVKRPENRFTIHGVWPSL-TSGNATKCSGTPPYDG 84
           +FD++  + QW  A C S  +   P   +P   F IHG+WP+  T G    C+    +D 
Sbjct: 21  DFDFFYFVLQWPGAYCDSRHSCCYPQTGKPAADFGIHGLWPNYKTGGWPQNCNPDSRFDD 80

Query: 85  NKIYAIRSRLDQDWPSVVYGTNPTL--WSHEWKTHGTCSNYYLDQYNYFNFGLSVFGKYN 144
            ++  + S L ++WP++   +N  +  W+HEW+ HGTC+   LDQ++YF  GL +  K N
Sbjct: 81  LRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESELDQHDYFEAGLKLKQKAN 140

Query: 145 MISILRKYGINPDGNDYSIDYIESAIYNATVKWPALRCNKNKSSGKKQLYEIALCFTKVN 204
           ++  L   GI PD   Y +  IE+ I       P + CNK+ SS   QLY+I LC     
Sbjct: 141 LLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNKD-SSHNSQLYQIYLCVDTSA 200

Query: 205 RALQDCP 208
               +CP
Sbjct: 201 SKFINCP 206

[Close]

BLAST of Bhi11G002482 vs. ExPASy Swiss-Prot
Match: Q7M438 (Ribonuclease DdI OS=Dictyostelium discoideum OX=44689 GN=ddiA PE=1 SV=3)

HSP 1 Score: 109.0 bits (271), Expect = 7.0e-23
Identity = 70/216 (32.41%), Postives = 108/216 (50.00%), Query Frame = 0


Query: 4   REIMLVLSILLL--------LVGSSKGLNEFDYYQVIFQWQPAACSSATLKSPCVKRPEN 63
           R I  +LS+LL+         +  S    +FD+Y  + QW  + C S T    C++  E 
Sbjct: 2   RLIAALLSVLLIASTAQSTVTIYESSKPGDFDFYLFVQQWIYSYCDSQT----CIQNKER 61

Query: 64  R-FTIHGVWPSLTSGNATKCSGTPPYDGNKIYAIRSRLDQDWPSVVYGTNPTLWSHEWKT 123
             FTIHG+WP  + G+       P ++ N I  +  +L+ DWPS+  G N   W+HE+  
Sbjct: 62  EAFTIHGLWPENSDGSYPSFCSGPSFNVNAIQDLEDQLNFDWPSLT-GPNTDFWTHEFSK 121

Query: 124 HGTCS--NYYLDQYNYFNFGLSVFGKYNMISILRKYGINP-DGNDYSIDYIESAIYNATV 183
           HGTCS      D ++YF  G+ ++ ++N+ + L    I P D N Y    I +AI     
Sbjct: 122 HGTCSITGPITDIHDYFATGIKLYTEFNITAALESENIYPSDSNTYKPVDITNAITTHFG 181

Query: 184 KWPALRCNKNKSSGKKQLYEIALCFTKVNRALQDCP 208
             P ++C    SSG  QL  +A+C  K + ++ DCP
Sbjct: 182 GKPGIQC----SSG--QLSTVAVCIDKNSLSIMDCP 206

[Close]

BLAST of Bhi11G002482 vs. ExPASy Swiss-Prot
Match: O80325 (Ribonuclease S-7 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 3.5e-22
Identity = 67/208 (32.21%), Postives = 113/208 (54.33%), Query Frame = 0


Query: 6   IMLVLSILLLLVGSSKGLNEFDYYQVIFQWQPAACSSATLKSPCVKRPENRFTIHGVWPS 65
           + +V S+++L++ SS     +DY+Q   Q+QPA C+S    +PC   P+  FT+HG+WPS
Sbjct: 10  VTMVFSLIVLILSSS--TVGYDYFQFTQQYQPAVCNSK--PTPCKDPPDKLFTVHGLWPS 69

Query: 66  LTSGNATKCSGTPPYDGNKIYAIRSRLDQDWPSVVYGTNPT-LWSHEWKTHGTCS-NYYL 125
             +G   +       + ++I  I+++L   WP+V+  TN    W+ +W  HG+C     +
Sbjct: 70  NLNGPHPENCTNATVNPHRIKNIQAQLKIIWPNVLDRTNHVGFWNKQWIKHGSCGYPAIM 129

Query: 126 DQYNYFNFGLSVF--GKYNMISILRKYGINPDGNDYSIDYIESAIYNAT-VKWPALRCNK 185
           +  +YF   ++++   K N+  IL K  I P G    + +IE+AI N+T  K P  +C K
Sbjct: 130 NDTHYFQTVINMYITQKQNVSEILSKAKIEPLGIQRPLVHIENAIRNSTNNKKPKFKCQK 189

Query: 186 NKSSGKKQLYEIALCFTKVNRALQDCPY 209
           N  SG  +L E+ LC        ++CP+
Sbjct: 190 N--SGVTELVEVGLCSDGSLTQFRNCPH 211

[Close]

BLAST of Bhi11G002482 vs. NCBI nr
Match: XP_038903348.1 (ribonuclease MC-like [Benincasa hispida])

HSP 1 Score: 476.5 bits (1225), Expect = 1.3e-130
Identity = 221/221 (100.00%), Postives = 221/221 (100.00%), Query Frame = 0


Query: 1   MGKREIMLVLSILLLLVGSSKGLNEFDYYQVIFQWQPAACSSATLKSPCVKRPENRFTIH 60
           MGKREIMLVLSILLLLVGSSKGLNEFDYYQVIFQWQPAACSSATLKSPCVKRPENRFTIH
Sbjct: 1   MGKREIMLVLSILLLLVGSSKGLNEFDYYQVIFQWQPAACSSATLKSPCVKRPENRFTIH 60

Query: 61  GVWPSLTSGNATKCSGTPPYDGNKIYAIRSRLDQDWPSVVYGTNPTLWSHEWKTHGTCSN 120
           GVWPSLTSGNATKCSGTPPYDGNKIYAIRSRLDQDWPSVVYGTNPTLWSHEWKTHGTCSN
Sbjct: 61  GVWPSLTSGNATKCSGTPPYDGNKIYAIRSRLDQDWPSVVYGTNPTLWSHEWKTHGTCSN 120

Query: 121 YYLDQYNYFNFGLSVFGKYNMISILRKYGINPDGNDYSIDYIESAIYNATVKWPALRCNK 180
           YYLDQYNYFNFGLSVFGKYNMISILRKYGINPDGNDYSIDYIESAIYNATVKWPALRCNK
Sbjct: 121 YYLDQYNYFNFGLSVFGKYNMISILRKYGINPDGNDYSIDYIESAIYNATVKWPALRCNK 180

Query: 181 NKSSGKKQLYEIALCFTKVNRALQDCPYNAVTCGNKFSWNS 222
           NKSSGKKQLYEIALCFTKVNRALQDCPYNAVTCGNKFSWNS
Sbjct: 181 NKSSGKKQLYEIALCFTKVNRALQDCPYNAVTCGNKFSWNS 221

[Close]

BLAST of Bhi11G002482 vs. NCBI nr
Match: XP_022158939.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 300.8 bits (769), Expect = 9.5e-78
Identity = 133/221 (60.18%), Postives = 171/221 (77.38%), Query Frame = 0


Query: 1   MGKREIMLVLSILLLLVGSSKGLNEFDYYQVIFQWQPAACSSATLKSPCVKRPENRFTIH 60
           MGKREI+LVLS + L + SS+G+NEFDYYQV+ QWQPA CSSATLK+ CV+ PE+R+++H
Sbjct: 1   MGKREILLVLS-MFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVH 60

Query: 61  GVWPSLTSGNATKCSGTPPYDGNKIYAIRSRLDQDWPSVVYGTNPTLWSHEWKTHGTCSN 120
           GVWPSL+SG    CSGTPPYD   I  I++RLDQDWP+V+ G NP  WS+EW  HGTCS+
Sbjct: 61  GVWPSLSSGYLPTCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSS 120

Query: 121 YYLDQYNYFNFGLSVFGKYNMISILRKYGINPDGNDYSIDYIESAIYNATVKWPALRCNK 180
            YL+Q++YFNFGL V+  Y++ SIL +YGI P+G  Y+   +++AIY+AT KWPALRCN 
Sbjct: 121 AYLNQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNN 180

Query: 181 NKSSGKKQLYEIALCFTKVNRALQDCPYNAVTCGNKFSWNS 222
           N  +G  QL+EI+LCF+K  + L DCP+N VTC   F WNS
Sbjct: 181 NYRTGNMQLHEISLCFSKNGQILIDCPFNVVTCNEYFVWNS 220

[Close]

BLAST of Bhi11G002482 vs. NCBI nr
Match: XP_022156540.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 288.5 bits (737), Expect = 4.9e-74
Identity = 135/223 (60.54%), Postives = 172/223 (77.13%), Query Frame = 0


Query: 1   MGKR-EIMLVLSILLLLVGSSKGLNEFDYYQVIFQWQPAACSSATLKSPCVKRPENRFTI 60
           M KR EI+L+LS +LLL+G S+G+NEFDYYQV+ QWQPA CSS+TL   CV+ P+ RF+I
Sbjct: 1   MAKRSEIVLILS-MLLLIGWSEGVNEFDYYQVVLQWQPATCSSSTLVDECVQPPDERFSI 60

Query: 61  HGVWPSLTSGNATKCSGTPPYDGNKI-YAIRSRLDQDWPSVVYGTNPTLWSHEWKTHGTC 120
           HGVWPSL SG+A  CSGTPPYDG ++  +IR+RL QDWPSVV+  +P  WSHEW  HGTC
Sbjct: 61  HGVWPSLLSGDAV-CSGTPPYDGTQLNSSIRARLHQDWPSVVHRVDPPFWSHEWTLHGTC 120

Query: 121 SNYYLDQYNYFNFGLSVFGKYNMISILRKYGINPDGNDYSIDYIESAIYNATVKWPALRC 180
           S  YLDQ++YF+FGL V+G Y+++ IL  Y + P+GN Y+   +E+ IY+AT KWPALRC
Sbjct: 121 SYAYLDQFHYFDFGLFVYGNYDVLGILNYYNVYPNGNLYARSDVENIIYSATGKWPALRC 180

Query: 181 NKNKSSGKKQLYEIALCFTKVNRALQDCPYNAVTCGNKFSWNS 222
           N N  SG  QL+EI+LCF K + +L+DCPYNAV C + F WNS
Sbjct: 181 NNNFWSGDVQLHEISLCFRKQDFSLEDCPYNAVKCNDYFVWNS 221

[Close]

BLAST of Bhi11G002482 vs. NCBI nr
Match: XP_022159354.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 257.7 bits (657), Expect = 9.2e-65
Identity = 120/219 (54.79%), Postives = 151/219 (68.95%), Query Frame = 0


Query: 1   MGKREIMLVLSILLLLVGSSKGLNEFDYYQVIFQWQPAACSSATLKSPCVKRPENRFTIH 60
           M K+ I+LV S L+++        +++YYQV  QWQPA CS A L++PCV  P++RF+IH
Sbjct: 1   MEKKGIVLVFSTLVVIASC-----DYNYYQVTLQWQPATCSDANLQTPCVNTPQDRFSIH 60

Query: 61  GVWPSLTSGNATKCSGTPPYDGNKIYAIRSRLDQDWPSVVYGTNPTLWSHEWKTHGTCSN 120
           GVWP+L+SG+  +C GTP YDG+KI  IR+RL+ DWPSV+YGTNPTLWSHEW THGTCS 
Sbjct: 61  GVWPTLSSGSYPQCPGTPAYDGSKISTIRNRLNADWPSVIYGTNPTLWSHEWTTHGTCSY 120

Query: 121 YYLDQYNYFNFGLSVFGKYNMISILRKYGINPDGNDYSIDYIESAIYNATVKWPALRCNK 180
             L Q +YF FGL  + KY++ S L K  I P  + YS   I +AI   T K P LRCN 
Sbjct: 121 PSLSQLDYFTFGLDTYAKYDIYSALDKEDIYPSEDVYSKSSINNAIRKITQKLPGLRCNT 180

Query: 181 NKSSGKKQLYEIALCFTKVNRALQDCPYNAVTCGNKFSW 220
           N  S ++QL+EI LCF K NRA+ DCP N VTCGN   W
Sbjct: 181 NDYSRERQLHEIILCFAKSNRAIIDCPSNVVTCGNYVYW 214

[Close]

BLAST of Bhi11G002482 vs. NCBI nr
Match: AEM42996.1 (S-RNase [Siraitia grosvenorii])

HSP 1 Score: 221.1 bits (562), Expect = 9.6e-54
Identity = 105/223 (47.09%), Postives = 149/223 (66.82%), Query Frame = 0


Query: 1   MGKREIMLVLSILLLLVGSSKGLNEFDYYQVIFQWQPAACSSATLKSP-CVKRPENRFTI 60
           M KREI+LVLS +L+L+ S +GLNEFDY QV+ QWQPA CS++    P C + P+NR++I
Sbjct: 1   MAKREIVLVLS-MLVLIASCEGLNEFDYLQVVMQWQPATCSASNKPYPICYQNPDNRYSI 60

Query: 61  HGVWPSLTSGNATKCSGTPPYDGNKIYAIRSRLDQDWPSVVYGTNPTLWSHEWKTHGTCS 120
           HGVWPSL SG AT+CSG+ P++ N I  ++  + + WP+++ G N  LW HEW  HGTC+
Sbjct: 61  HGVWPSLYSGAATQCSGS-PFNANAISILQPGISEIWPNIINGNNLWLWGHEWDKHGTCT 120

Query: 121 NYYL-DQYNYFNFGLSVFGKYNMISILRKYGINPDGNDYSIDYIESAIYNATVKWPALRC 180
              L DQ  YF+F +  +  YN++ +L    I P+G  YS D + +AI   T K PA+RC
Sbjct: 121 EEILFDQDRYFSFAMDTYIDYNLLVLLGNSQITPNGQIYSRDQVYAAIRAKTGKTPAVRC 180

Query: 181 NKNKSSGKKQLYEIALCFTKVNRALQDCPYNAVTCGNKFSWNS 222
           N N+ +G++Q++E++LC+     A+ DCP NA  C N F W S
Sbjct: 181 NYNRWTGEQQMHEVSLCYNHDASAVVDCPLNANKCDNYFVWYS 221

[Close]

BLAST of Bhi11G002482 vs. ExPASy TrEMBL
Match: A0A6J1E0X1 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025395 PE=3 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 4.6e-78
Identity = 133/221 (60.18%), Postives = 171/221 (77.38%), Query Frame = 0


Query: 1   MGKREIMLVLSILLLLVGSSKGLNEFDYYQVIFQWQPAACSSATLKSPCVKRPENRFTIH 60
           MGKREI+LVLS + L + SS+G+NEFDYYQV+ QWQPA CSSATLK+ CV+ PE+R+++H
Sbjct: 1   MGKREILLVLS-MFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVH 60

Query: 61  GVWPSLTSGNATKCSGTPPYDGNKIYAIRSRLDQDWPSVVYGTNPTLWSHEWKTHGTCSN 120
           GVWPSL+SG    CSGTPPYD   I  I++RLDQDWP+V+ G NP  WS+EW  HGTCS+
Sbjct: 61  GVWPSLSSGYLPTCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSS 120

Query: 121 YYLDQYNYFNFGLSVFGKYNMISILRKYGINPDGNDYSIDYIESAIYNATVKWPALRCNK 180
            YL+Q++YFNFGL V+  Y++ SIL +YGI P+G  Y+   +++AIY+AT KWPALRCN 
Sbjct: 121 AYLNQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNN 180

Query: 181 NKSSGKKQLYEIALCFTKVNRALQDCPYNAVTCGNKFSWNS 222
           N  +G  QL+EI+LCF+K  + L DCP+N VTC   F WNS
Sbjct: 181 NYRTGNMQLHEISLCFSKNGQILIDCPFNVVTCNEYFVWNS 220

[Close]

BLAST of Bhi11G002482 vs. ExPASy TrEMBL
Match: A0A6J1DQK2 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111023418 PE=3 SV=1)

HSP 1 Score: 288.5 bits (737), Expect = 2.4e-74
Identity = 135/223 (60.54%), Postives = 172/223 (77.13%), Query Frame = 0


Query: 1   MGKR-EIMLVLSILLLLVGSSKGLNEFDYYQVIFQWQPAACSSATLKSPCVKRPENRFTI 60
           M KR EI+L+LS +LLL+G S+G+NEFDYYQV+ QWQPA CSS+TL   CV+ P+ RF+I
Sbjct: 1   MAKRSEIVLILS-MLLLIGWSEGVNEFDYYQVVLQWQPATCSSSTLVDECVQPPDERFSI 60

Query: 61  HGVWPSLTSGNATKCSGTPPYDGNKI-YAIRSRLDQDWPSVVYGTNPTLWSHEWKTHGTC 120
           HGVWPSL SG+A  CSGTPPYDG ++  +IR+RL QDWPSVV+  +P  WSHEW  HGTC
Sbjct: 61  HGVWPSLLSGDAV-CSGTPPYDGTQLNSSIRARLHQDWPSVVHRVDPPFWSHEWTLHGTC 120

Query: 121 SNYYLDQYNYFNFGLSVFGKYNMISILRKYGINPDGNDYSIDYIESAIYNATVKWPALRC 180
           S  YLDQ++YF+FGL V+G Y+++ IL  Y + P+GN Y+   +E+ IY+AT KWPALRC
Sbjct: 121 SYAYLDQFHYFDFGLFVYGNYDVLGILNYYNVYPNGNLYARSDVENIIYSATGKWPALRC 180

Query: 181 NKNKSSGKKQLYEIALCFTKVNRALQDCPYNAVTCGNKFSWNS 222
           N N  SG  QL+EI+LCF K + +L+DCPYNAV C + F WNS
Sbjct: 181 NNNFWSGDVQLHEISLCFRKQDFSLEDCPYNAVKCNDYFVWNS 221

[Close]

BLAST of Bhi11G002482 vs. ExPASy TrEMBL
Match: A0A6J1E266 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025771 PE=3 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 4.5e-65
Identity = 120/219 (54.79%), Postives = 151/219 (68.95%), Query Frame = 0


Query: 1   MGKREIMLVLSILLLLVGSSKGLNEFDYYQVIFQWQPAACSSATLKSPCVKRPENRFTIH 60
           M K+ I+LV S L+++        +++YYQV  QWQPA CS A L++PCV  P++RF+IH
Sbjct: 1   MEKKGIVLVFSTLVVIASC-----DYNYYQVTLQWQPATCSDANLQTPCVNTPQDRFSIH 60

Query: 61  GVWPSLTSGNATKCSGTPPYDGNKIYAIRSRLDQDWPSVVYGTNPTLWSHEWKTHGTCSN 120
           GVWP+L+SG+  +C GTP YDG+KI  IR+RL+ DWPSV+YGTNPTLWSHEW THGTCS 
Sbjct: 61  GVWPTLSSGSYPQCPGTPAYDGSKISTIRNRLNADWPSVIYGTNPTLWSHEWTTHGTCSY 120

Query: 121 YYLDQYNYFNFGLSVFGKYNMISILRKYGINPDGNDYSIDYIESAIYNATVKWPALRCNK 180
             L Q +YF FGL  + KY++ S L K  I P  + YS   I +AI   T K P LRCN 
Sbjct: 121 PSLSQLDYFTFGLDTYAKYDIYSALDKEDIYPSEDVYSKSSINNAIRKITQKLPGLRCNT 180

Query: 181 NKSSGKKQLYEIALCFTKVNRALQDCPYNAVTCGNKFSW 220
           N  S ++QL+EI LCF K NRA+ DCP N VTCGN   W
Sbjct: 181 NDYSRERQLHEIILCFAKSNRAIIDCPSNVVTCGNYVYW 214

[Close]

BLAST of Bhi11G002482 vs. ExPASy TrEMBL
Match: K7NBR4 (S-RNase OS=Siraitia grosvenorii OX=190515 PE=2 SV=1)

HSP 1 Score: 221.1 bits (562), Expect = 4.6e-54
Identity = 105/223 (47.09%), Postives = 149/223 (66.82%), Query Frame = 0


Query: 1   MGKREIMLVLSILLLLVGSSKGLNEFDYYQVIFQWQPAACSSATLKSP-CVKRPENRFTI 60
           M KREI+LVLS +L+L+ S +GLNEFDY QV+ QWQPA CS++    P C + P+NR++I
Sbjct: 1   MAKREIVLVLS-MLVLIASCEGLNEFDYLQVVMQWQPATCSASNKPYPICYQNPDNRYSI 60

Query: 61  HGVWPSLTSGNATKCSGTPPYDGNKIYAIRSRLDQDWPSVVYGTNPTLWSHEWKTHGTCS 120
           HGVWPSL SG AT+CSG+ P++ N I  ++  + + WP+++ G N  LW HEW  HGTC+
Sbjct: 61  HGVWPSLYSGAATQCSGS-PFNANAISILQPGISEIWPNIINGNNLWLWGHEWDKHGTCT 120

Query: 121 NYYL-DQYNYFNFGLSVFGKYNMISILRKYGINPDGNDYSIDYIESAIYNATVKWPALRC 180
              L DQ  YF+F +  +  YN++ +L    I P+G  YS D + +AI   T K PA+RC
Sbjct: 121 EEILFDQDRYFSFAMDTYIDYNLLVLLGNSQITPNGQIYSRDQVYAAIRAKTGKTPAVRC 180

Query: 181 NKNKSSGKKQLYEIALCFTKVNRALQDCPYNAVTCGNKFSWNS 222
           N N+ +G++Q++E++LC+     A+ DCP NA  C N F W S
Sbjct: 181 NYNRWTGEQQMHEVSLCYNHDASAVVDCPLNANKCDNYFVWYS 221

[Close]

BLAST of Bhi11G002482 vs. ExPASy TrEMBL
Match: A0A6J1D7Q5 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111018138 PE=3 SV=1)

HSP 1 Score: 215.7 bits (548), Expect = 1.9e-52
Identity = 105/222 (47.30%), Postives = 139/222 (62.61%), Query Frame = 0


Query: 1   MGKREIMLVLSILLLLVGSSKGLNEFDYYQVIFQWQPAACSSATLKSPCVKRPENRFTIH 60
           M +REI++++  +L+LV   +GLNEFDY QV+ QWQPA CS    K  C + P NRF+IH
Sbjct: 1   MAQREIIVLVLSMLVLVAICEGLNEFDYLQVVIQWQPATCSDYN-KPACFQAPGNRFSIH 60

Query: 61  GVWPSLTSGNATKCSGTPPYDGNKIYAIRSRLDQDWPSVVYGTNPTLWSHEWKTHGTCS- 120
           GVWPSL  G  T+CSG+ P+D N+I  ++  + + WP+V  G N  LW HEW  HGTCS 
Sbjct: 61  GVWPSLYDGYPTQCSGS-PFDRNQISGLQPGISEIWPNVATGDNFWLWQHEWDKHGTCSE 120

Query: 121 NYYLDQYNYFNFGLSVFGKYNMISILRKYGINPDGNDYSIDYIESAIYNATVKWPALRCN 180
           + + DQ  YF FG+  +   N+  IL    I P+G  Y+ D + +AI   T K PA+RCN
Sbjct: 121 STFYDQQEYFGFGMDAYIHNNLQVILSNNQITPNGRTYTRDQVYAAIRAKTGKTPAVRCN 180

Query: 181 KNKSSGKKQLYEIALCFTKVNRALQDCPYNAVTCGNKFSWNS 222
            N  +G  QL+EI+LCF K     +DCP NA  CG  F WNS
Sbjct: 181 YNSRTGASQLHEISLCFKKDASVFEDCPLNANKCGASFVWNS 220

The following BLAST results are available for this feature:

BLAST of Bhi11G002482 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G02990.1	4.9e-32	32.69	ribonuclease 1	[more]
AT1G14220.1	3.0e-29	32.86	Ribonuclease T2 family protein	[more]
AT1G26820.1	5.6e-28	34.22	ribonuclease 3	[more]
AT2G39780.1	3.3e-20	29.38	ribonuclease 2	[more]

BLAST of Bhi11G002482 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P42813	6.9e-31	32.69	Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1	[more]
P23540	2.5e-28	33.85	Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1	[more]
P42815	7.9e-27	34.22	Ribonuclease 3 OS=Arabidopsis thaliana OX=3702 GN=RNS3 PE=2 SV=1	[more]
Q7M438	7.0e-23	32.41	Ribonuclease DdI OS=Dictyostelium discoideum OX=44689 GN=ddiA PE=1 SV=3	[more]
O80325	3.5e-22	32.21	Ribonuclease S-7 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1	[more]

BLAST of Bhi11G002482 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038903348.1	1.3e-130	100.00	ribonuclease MC-like [Benincasa hispida]	[more]
XP_022158939.1	9.5e-78	60.18	ribonuclease MC-like [Momordica charantia]	[more]
XP_022156540.1	4.9e-74	60.54	ribonuclease MC-like [Momordica charantia]	[more]
XP_022159354.1	9.2e-65	54.79	ribonuclease MC-like [Momordica charantia]	[more]
AEM42996.1	9.6e-54	47.09	S-RNase [Siraitia grosvenorii]	[more]

BLAST of Bhi11G002482 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E0X1	4.6e-78	60.18	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025395 PE=3 SV=1	[more]
A0A6J1DQK2	2.4e-74	60.54	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111023418 PE=3 SV=1	[more]
A0A6J1E266	4.5e-65	54.79	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025771 PE=3 SV=1	[more]
K7NBR4	4.6e-54	47.09	S-RNase OS=Siraitia grosvenorii OX=190515 PE=2 SV=1	[more]
A0A6J1D7Q5	1.9e-52	47.30	ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111018138 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036430	Ribonuclease T2-like superfamily	GENE3D	3.90.730.10		coord: 20..219 e-value: 1.6E-52 score: 180.6
IPR036430	Ribonuclease T2-like superfamily	SUPERFAMILY	55895	Ribonuclease Rh-like	coord: 6..215
IPR001568	Ribonuclease T2-like	PFAM	PF00445	Ribonuclease_T2	coord: 26..206 e-value: 5.7E-40 score: 137.4
IPR001568	Ribonuclease T2-like	PANTHER	PTHR11240	RIBONUCLEASE T2	coord: 13..208
None	No IPR available	PANTHER	PTHR11240:SF49	RIBONUCLEASE 3	coord: 13..208
IPR033130	Ribonuclease T2, His active site 2	PROSITE	PS00531	RNASE_T2_2	coord: 107..118
IPR033697	Ribonuclease T2, eukaryotic	CDD	cd01061	RNase_T2_euk	coord: 26..207 e-value: 1.40808E-51 score: 163.657

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi11M002482	Bhi11M002482	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
biological_process	GO:0016070	RNA metabolic process
molecular_function	GO:0033897	ribonuclease T2 activity
molecular_function	GO:0003723	RNA binding