Bhi11G002433 (gene) Wax gourd (B227) v1

Overview


Name	Bhi11G002433
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Calcium-binding EF-hand family protein
Location	chr11: 79078930 .. 79079985 (+)
RNA-Seq Expression	Bhi11G002433
Synteny	Bhi11G002433

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTAATTTCTTATATTTTATAATATTGAAAGAGTATTACCGTTTCTTCCCACGAAAAATAATAAAAATGGGGGCGAAAAAGAAAAGAAAAGAAAAAGAGAGTTAGAAAGGAAGTTTCCGGGAAGTTGCATAAGGGGTAGTACACAATTTTCAGAGTTTCTCCTTCTCTCCCACCATATAACCCCCTTCATCTTCTTCACTCTAATTGCCCCAAATTCCCTTCTTCCCAAATCAAATTCAATTTTCTTCATTTCATTTCCATTTCCATTTCAAAATGAAGCTCCTCAAATCCTTCAATCCCAACCGTCTCTTCTCTTCCAAATCCCACCGCTCCAAACTCTCCCGATCCCAACCTCCCTCCTTCGGCTCCGCCTCCTCCTCCTCCTCCGCCGGCACTCCCACCAGCGTCTTACCTTCCGCCGTCTGGTCCGACTTCTCCGACTCCACACTCTCTCGCAAGGAACTGGAGGCTTTACTCGGCCTAATCGGAGCCGAACCGCCGAGTGAGGAAGAGCTTAAGACTATGATGGGCGCAATGGACCGTGTCGGACCGGCTTGCCACTCTGAGCTACGAGATACGTTTGAGATATTCGACGCTGATCACGATGGGAGAATTACGGCGGAGGAGCTTTTCAGTGTGTTCACGGCTATGGGGGATGAGCAGTGCACGTTAGAGGATTGCCGGCGTATGATAGCGGGAGTGGACAAGAATGGAGATGGGTTCGTCTGCTTCGAGGACTTTGCGCGTATGATGGACTGCCAGAGATGAACCCAATCATCACTGTTCATTTTTTTCGTTTAATTATTACTAATAATAATAATTTTTTTTTAAGAAAAAAGATCTTTGTATTTGCGTTGGTTGTTGTTAAGTATATAAAAACGGCGCTAAGTCGTCGGATCGGACAACATTGGTCAAATCATCCCTTCTCCGATTTCTTTTTCTTTTTCCTTCTTATTTCGCATGTTTATTTAATTACTTCTAATTAAAAAACTATTTTTATCCAATTATATATTTTTAAATATTCATGTTTAATTCCATGTCATGAAACATAAATACA

mRNA sequence

GTAATTTCTTATATTTTATAATATTGAAAGAGTATTACCGTTTCTTCCCACGAAAAATAATAAAAATGGGGGCGAAAAAGAAAAGAAAAGAAAAAGAGAGTTAGAAAGGAAGTTTCCGGGAAGTTGCATAAGGGGTAGTACACAATTTTCAGAGTTTCTCCTTCTCTCCCACCATATAACCCCCTTCATCTTCTTCACTCTAATTGCCCCAAATTCCCTTCTTCCCAAATCAAATTCAATTTTCTTCATTTCATTTCCATTTCCATTTCAAAATGAAGCTCCTCAAATCCTTCAATCCCAACCGTCTCTTCTCTTCCAAATCCCACCGCTCCAAACTCTCCCGATCCCAACCTCCCTCCTTCGGCTCCGCCTCCTCCTCCTCCTCCGCCGGCACTCCCACCAGCGTCTTACCTTCCGCCGTCTGGTCCGACTTCTCCGACTCCACACTCTCTCGCAAGGAACTGGAGGCTTTACTCGGCCTAATCGGAGCCGAACCGCCGAGTGAGGAAGAGCTTAAGACTATGATGGGCGCAATGGACCGTGTCGGACCGGCTTGCCACTCTGAGCTACGAGATACGTTTGAGATATTCGACGCTGATCACGATGGGAGAATTACGGCGGAGGAGCTTTTCAGTGTGTTCACGGCTATGGGGGATGAGCAGTGCACGTTAGAGGATTGCCGGCGTATGATAGCGGGAGTGGACAAGAATGGAGATGGGTTCGTCTGCTTCGAGGACTTTGCGCGTATGATGGACTGCCAGAGATGAACCCAATCATCACTGTTCATTTTTTTCGTTTAATTATTACTAATAATAATAATTTTTTTTTAAGAAAAAAGATCTTTGTATTTGCGTTGGTTGTTGTTAAGTATATAAAAACGGCGCTAAGTCGTCGGATCGGACAACATTGGTCAAATCATCCCTTCTCCGATTTCTTTTTCTTTTTCCTTCTTATTTCGCATGTTTATTTAATTACTTCTAATTAAAAAACTATTTTTATCCAATTATATATTTTTAAATATTCATGTTTAATTCCATGTCATGAAACATAAATACA

Coding sequence (CDS)

ATGAAGCTCCTCAAATCCTTCAATCCCAACCGTCTCTTCTCTTCCAAATCCCACCGCTCCAAACTCTCCCGATCCCAACCTCCCTCCTTCGGCTCCGCCTCCTCCTCCTCCTCCGCCGGCACTCCCACCAGCGTCTTACCTTCCGCCGTCTGGTCCGACTTCTCCGACTCCACACTCTCTCGCAAGGAACTGGAGGCTTTACTCGGCCTAATCGGAGCCGAACCGCCGAGTGAGGAAGAGCTTAAGACTATGATGGGCGCAATGGACCGTGTCGGACCGGCTTGCCACTCTGAGCTACGAGATACGTTTGAGATATTCGACGCTGATCACGATGGGAGAATTACGGCGGAGGAGCTTTTCAGTGTGTTCACGGCTATGGGGGATGAGCAGTGCACGTTAGAGGATTGCCGGCGTATGATAGCGGGAGTGGACAAGAATGGAGATGGGTTCGTCTGCTTCGAGGACTTTGCGCGTATGATGGACTGCCAGAGATGA

Protein sequence

MKLLKSFNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSAGTPTSVLPSAVWSDFSDSTLSRKELEALLGLIGAEPPSEEELKTMMGAMDRVGPACHSELRDTFEIFDADHDGRITAEELFSVFTAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFARMMDCQR

Homology

[Close]

BLAST of Bhi11G002433 vs. TAIR 10
Match: AT3G10190.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 129.0 bits (323), Expect = 3.4e-30
Identity = 94/211 (44.55%), Postives = 120/211 (56.87%), Query Frame = 0


Query: 1   MKLLKSFNPNRLFSSKSHRSKLSRSQPP-SFGSASSSS------SAG-------TPTSVL 60
           MKL K   P R F     RS +S+S    SFGSASSSS      S G       TPTS+L
Sbjct: 1   MKLAKLI-PKRFFIRSKDRSTVSKSPTAFSFGSASSSSGQDCKNSGGDGGGGSVTPTSIL 60

Query: 61  PSAVWSDFS---------------DSTLSRKELEALLGLIGAEPPSEEELKTMMGAMDRV 120
           P  V S +S               D  +SR +LE+LL  +G +P +EEE+  M+  +D  
Sbjct: 61  PE-VPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEEEINVMLKEVDCD 120

Query: 121 G-----------------PACHS-ELRDTFEIFDADHDGRITAEELFSVFTAMGDEQCTL 165
           G                 PA  S EL++TFE FDAD DG I+A+EL  VF+ +GDE+CTL
Sbjct: 121 GDGTIRLEELASRVVSLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIGDERCTL 180

[Close]

BLAST of Bhi11G002433 vs. TAIR 10
Match: AT2G41410.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 128.3 bits (321), Expect = 5.8e-30
Identity = 93/209 (44.50%), Postives = 114/209 (54.55%), Query Frame = 0


Query: 1   MKLLKSFN---PNRLFSSKSHRSKLSRSQPPSFGS-ASSSSSAG-----------TPTSV 60
           MKL  S N   P RLF +KS ++ +SRS+P SF S ASSSSS G           TP SV
Sbjct: 1   MKLAASLNRLSPKRLFRTKS-KASVSRSEPSSFSSNASSSSSDGSYGNLKQGPTATPISV 60

Query: 61  LPSAVWSDF--------------SDSTLSRKELEALLGLIGAEPPSEEELKTMMGAMDRV 120
           LP     DF               D  +SR +L AL+  +  EPPS+EE+  M+  +D  
Sbjct: 61  LPQNS-GDFYTELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGG 120

Query: 121 GPACHS--------------------ELRDTFEIFDADHDGRITAEELFSVFTAMGDEQC 161
              C S                    ELR+ FEIFD D +G+I+AEEL  VF  +GDE+C
Sbjct: 121 DGGCISLEDLASRVAGTSGEGSVETEELREVFEIFDVDRNGKISAEELHRVFGVIGDERC 180

[Close]

BLAST of Bhi11G002433 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 82.0 bits (201), Expect = 4.8e-16
Identity = 48/124 (38.71%), Postives = 69/124 (55.65%), Query Frame = 0


Query: 56  DSTLSRKELEALLGLIGAEPPSEEELKTMMGAMDRVGPA-------------------CH 115
           D  +S KEL A++  +G E P EEEL+  +  +DR G                       
Sbjct: 50  DGKISSKELGAIMTSLGHEVP-EEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVL 109

Query: 116 SELRDTFEIFDADHDGRITAEELFSVFTAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDF 161
             L+D F ++D D +G I+AEEL  V  ++GDE C++ +CR+MI GVDK+GDG + FE+F
Sbjct: 110 ENLKDAFSVYDIDGNGSISAEELHEVLRSLGDE-CSIAECRKMIGGVDKDGDGTIDFEEF 169

[Close]

BLAST of Bhi11G002433 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 73.6 bits (179), Expect = 1.7e-13
Identity = 46/134 (34.33%), Postives = 71/134 (52.99%), Query Frame = 0


Query: 50  VWSDFSDSTLSRKELEALLGLIGAEPP--SEEELKTMMGAMDRVG--------------- 109
           V+  F  +   +  ++ L  +IGA  P  S+EE K MM   D  G               
Sbjct: 19  VFQRFDKNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVALFQI 78

Query: 110 ------PACHSELRDTFEIFDADHDGRITAEELFSVFTAMGDEQCTLEDCRRMIAGVDKN 161
                  +   +L++ F+++D D +GRI+A EL SV   +G E+C+++DC+RMI  VD +
Sbjct: 79  SDQSSNNSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLG-EKCSIQDCQRMINKVDSD 138

[Close]

BLAST of Bhi11G002433 vs. TAIR 10
Match: AT2G15680.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 72.4 bits (176), Expect = 3.8e-13
Identity = 55/171 (32.16%), Postives = 83/171 (48.54%), Query Frame = 0


Query: 9   PNRLFSSKSHRSKLSRSQPPSFGSASSSSSAGTPTSVLPSAVWSDF---SDSTLSRKELE 68
           P+R+FS     S LS   P  F   S +             V+S F    D  +S+ E +
Sbjct: 22  PSRMFSRDRQSSGLSSPGPGGFSQPSVNEM---------RRVFSRFDLDKDGKISQTEYK 81

Query: 69  ALLGLIGAEPPSEE----------------ELKTMMGAMDRVGPACHSELRDTFEIFDAD 128
            +L  +G E   E+                + +  + A  R G    S++R++F  FD +
Sbjct: 82  VVLRALGQERAIEDVPKIFKAVDLDGDGFIDFREFIDAYKRSGGIRSSDIRNSFWTFDLN 141

Query: 129 HDGRITAEELFSVFTAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFARMM 161
            DG+I+AEE+ SV   +G E+C+LEDC RM+  VD +GDG V  E+F +MM
Sbjct: 142 GDGKISAEEVMSVLWKLG-ERCSLEDCNRMVRAVDADGDGLVNMEEFIKMM 182

[Close]

BLAST of Bhi11G002433 vs. ExPASy Swiss-Prot
Match: Q9SS31 (Probable calcium-binding protein CML36 OS=Arabidopsis thaliana OX=3702 GN=CML36 PE=2 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 4.8e-29
Identity = 94/211 (44.55%), Postives = 120/211 (56.87%), Query Frame = 0


Query: 1   MKLLKSFNPNRLFSSKSHRSKLSRSQPP-SFGSASSSS------SAG-------TPTSVL 60
           MKL K   P R F     RS +S+S    SFGSASSSS      S G       TPTS+L
Sbjct: 1   MKLAKLI-PKRFFIRSKDRSTVSKSPTAFSFGSASSSSGQDCKNSGGDGGGGSVTPTSIL 60

Query: 61  PSAVWSDFS---------------DSTLSRKELEALLGLIGAEPPSEEELKTMMGAMDRV 120
           P  V S +S               D  +SR +LE+LL  +G +P +EEE+  M+  +D  
Sbjct: 61  PE-VPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEEEINVMLKEVDCD 120

Query: 121 G-----------------PACHS-ELRDTFEIFDADHDGRITAEELFSVFTAMGDEQCTL 165
           G                 PA  S EL++TFE FDAD DG I+A+EL  VF+ +GDE+CTL
Sbjct: 121 GDGTIRLEELASRVVSLDPARDSTELKETFEFFDADRDGLISADELLRVFSTIGDERCTL 180

[Close]

BLAST of Bhi11G002433 vs. ExPASy Swiss-Prot
Match: P30188 (Probable calcium-binding protein CML35 OS=Arabidopsis thaliana OX=3702 GN=CML35 PE=2 SV=2)

HSP 1 Score: 128.3 bits (321), Expect = 8.2e-29
Identity = 93/209 (44.50%), Postives = 114/209 (54.55%), Query Frame = 0


Query: 1   MKLLKSFN---PNRLFSSKSHRSKLSRSQPPSFGS-ASSSSSAG-----------TPTSV 60
           MKL  S N   P RLF +KS ++ +SRS+P SF S ASSSSS G           TP SV
Sbjct: 1   MKLAASLNRLSPKRLFRTKS-KASVSRSEPSSFSSNASSSSSDGSYGNLKQGPTATPISV 60

Query: 61  LPSAVWSDF--------------SDSTLSRKELEALLGLIGAEPPSEEELKTMMGAMDRV 120
           LP     DF               D  +SR +L AL+  +  EPPS+EE+  M+  +D  
Sbjct: 61  LPQNS-GDFYTELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGG 120

Query: 121 GPACHS--------------------ELRDTFEIFDADHDGRITAEELFSVFTAMGDEQC 161
              C S                    ELR+ FEIFD D +G+I+AEEL  VF  +GDE+C
Sbjct: 121 DGGCISLEDLASRVAGTSGEGSVETEELREVFEIFDVDRNGKISAEELHRVFGVIGDERC 180

[Close]

BLAST of Bhi11G002433 vs. ExPASy Swiss-Prot
Match: Q9FYK2 (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 6.8e-15
Identity = 48/124 (38.71%), Postives = 69/124 (55.65%), Query Frame = 0


Query: 56  DSTLSRKELEALLGLIGAEPPSEEELKTMMGAMDRVGPA-------------------CH 115
           D  +S KEL A++  +G E P EEEL+  +  +DR G                       
Sbjct: 50  DGKISSKELGAIMTSLGHEVP-EEELEKAITEIDRKGDGYINFEEFVELNTKGMDQNDVL 109

Query: 116 SELRDTFEIFDADHDGRITAEELFSVFTAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDF 161
             L+D F ++D D +G I+AEEL  V  ++GDE C++ +CR+MI GVDK+GDG + FE+F
Sbjct: 110 ENLKDAFSVYDIDGNGSISAEELHEVLRSLGDE-CSIAECRKMIGGVDKDGDGTIDFEEF 169

[Close]

BLAST of Bhi11G002433 vs. ExPASy Swiss-Prot
Match: Q5ZCK5 (Probable calcium-binding protein CML16 OS=Oryza sativa subsp. japonica OX=39947 GN=CML16 PE=2 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 8.3e-13
Identity = 53/128 (41.41%), Postives = 67/128 (52.34%), Query Frame = 0


Query: 56  DSTLSRKELEALLGLIGAEPPSE----EELKTMMGAMDR------------------VGP 115
           D  +S  EL A+   I A PPSE     E+  MM  +D                    G 
Sbjct: 40  DGRISPSELAAVTRAI-APPPSESAGGREVAAMMNELDTDRDGFVDLGEFAAFHGRGRGD 99

Query: 116 ACH-SELRDTFEIFDADHDGRITAEELFSVFTAMGDEQCTLEDCRRMIAGVDKNGDGFVC 161
           A H +ELR  F+++D D DGRITA EL  V   +G E C+ E+C RMIA VD +GDG V 
Sbjct: 100 AEHEAELRAAFDVYDVDGDGRITAAELGKVLGRIG-EGCSAEECERMIASVDVDGDGCVG 159

[Close]

BLAST of Bhi11G002433 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 2.4e-12
Identity = 56/164 (34.15%), Postives = 76/164 (46.34%), Query Frame = 0


Query: 23  SRSQPPSFGSASSSSSAGTPTSVLPSAVWSDF------SDSTLSRKELEALLGLIGAEPP 82
           S S+ P    A  +S  G+P       +   F       D  +SR EL AL   +G    
Sbjct: 14  SGSKSPPLPQADPASGGGSPAPTPEEEMERVFRKFDANGDGRISRSELGALFESLG-HAA 73

Query: 83  SEEELKTMMGAMDRVG--------------------PACHSELRDTFEIFDADHDGRITA 142
           +++EL  MM   D  G                     A   +LR  F +FDAD +G I+A
Sbjct: 74  TDDELARMMAEADADGDGFISLDEFAALNATASGDAAAVEEDLRHAFRVFDADGNGTISA 133

Query: 143 EELFSVFTAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFARMM 161
            EL  V   +G E+ T++ CRRMI GVD+NGDG + FE+F  MM
Sbjct: 134 AELARVLHGLG-EKATVQQCRRMIEGVDQNGDGLISFEEFKVMM 175

[Close]

BLAST of Bhi11G002433 vs. NCBI nr
Match: XP_038903370.1 (probable calcium-binding protein CML36 [Benincasa hispida])

HSP 1 Score: 322.8 bits (826), Expect = 1.7e-84
Identity = 164/164 (100.00%), Postives = 164/164 (100.00%), Query Frame = 0


Query: 1   MKLLKSFNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSAGTPTSVLPSAVWSDFSDSTLS 60
           MKLLKSFNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSAGTPTSVLPSAVWSDFSDSTLS
Sbjct: 1   MKLLKSFNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSAGTPTSVLPSAVWSDFSDSTLS 60

Query: 61  RKELEALLGLIGAEPPSEEELKTMMGAMDRVGPACHSELRDTFEIFDADHDGRITAEELF 120
           RKELEALLGLIGAEPPSEEELKTMMGAMDRVGPACHSELRDTFEIFDADHDGRITAEELF
Sbjct: 61  RKELEALLGLIGAEPPSEEELKTMMGAMDRVGPACHSELRDTFEIFDADHDGRITAEELF 120

Query: 121 SVFTAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFARMMDCQR 165
           SVFTAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFARMMDCQR
Sbjct: 121 SVFTAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFARMMDCQR 164

[Close]

BLAST of Bhi11G002433 vs. NCBI nr
Match: XP_008443339.2 (PREDICTED: probable calcium-binding protein CML35 [Cucumis melo] >KAA0053814.1 putative calcium-binding protein CML35 [Cucumis melo var. makuwa] >TYK25587.1 putative calcium-binding protein CML35 [Cucumis melo var. makuwa])

HSP 1 Score: 298.9 bits (764), Expect = 2.7e-77
Identity = 154/168 (91.67%), Postives = 158/168 (94.05%), Query Frame = 0


Query: 1   MKLLKSFNPNRLFSSKSHRSKLSRSQPPSFGSASSS----SSAGTPTSVLPSAVWSDFSD 60
           MKLLKSFNPNRLFSSKSHRSKLSRSQPPSFGSASSS    SSAGTPTSVLPS  W+DFSD
Sbjct: 1   MKLLKSFNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSSTSSAGTPTSVLPSLDWADFSD 60

Query: 61  STLSRKELEALLGLIGAEPPSEEELKTMMGAMDRVGPACHSELRDTFEIFDADHDGRITA 120
            TLSRKEL+ALLGLIGAEPPSEEE+K MMG MDRVGPACHSELRDTFEIFDADHDGRITA
Sbjct: 61  PTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPACHSELRDTFEIFDADHDGRITA 120

Query: 121 EELFSVFTAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFARMMDCQR 165
           EELFSVFTAMGDE CTLEDCRRMIAGVDKNGDGFVCF+DFARMMDCQR
Sbjct: 121 EELFSVFTAMGDEGCTLEDCRRMIAGVDKNGDGFVCFDDFARMMDCQR 168

[Close]

BLAST of Bhi11G002433 vs. NCBI nr
Match: XP_004136671.2 (probable calcium-binding protein CML35 [Cucumis sativus] >KGN59507.1 hypothetical protein Csa_001340 [Cucumis sativus])

HSP 1 Score: 290.4 bits (742), Expect = 9.5e-75
Identity = 149/167 (89.22%), Postives = 154/167 (92.22%), Query Frame = 0


Query: 1   MKLLKSFNPNRLFSSKSHRSKLSRSQPPSFGSASSSSS---AGTPTSVLPSAVWSDFSDS 60
           MKLLKS NPNRLFSSKSHRSKLSRSQPPSFGSASSSSS   AGTPTSVLPS  WSDFSD 
Sbjct: 1   MKLLKSLNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSSSAAGTPTSVLPSLDWSDFSDP 60

Query: 61  TLSRKELEALLGLIGAEPPSEEELKTMMGAMDRVGPACHSELRDTFEIFDADHDGRITAE 120
           TLSRKEL+ALLGLIGAEPPSEEE+K MMG MDRVGP CHSELRDTFEIFDADHDGRITAE
Sbjct: 61  TLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPTCHSELRDTFEIFDADHDGRITAE 120

Query: 121 ELFSVFTAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFARMMDCQR 165
           ELFSVF AMGD+ CTLEDC+RMIAGVDKNGDGFVCF+DF RMMDCQR
Sbjct: 121 ELFSVFAAMGDDGCTLEDCQRMIAGVDKNGDGFVCFDDFVRMMDCQR 167

[Close]

BLAST of Bhi11G002433 vs. NCBI nr
Match: KAG6580931.1 (putative calcium-binding protein CML36, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017674.1 putative calcium-binding protein CML36, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 251.5 bits (641), Expect = 4.9e-63
Identity = 134/168 (79.76%), Postives = 143/168 (85.12%), Query Frame = 0


Query: 5   KSFNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSAGTPTSVLP-------SAVWSDFSDS 64
           KSF+P RLF SK HR+ LS+S+PPSFGSASSSS    PTSVLP       S  WSDFSD+
Sbjct: 9   KSFSPGRLFPSKKHRTSLSKSEPPSFGSASSSS----PTSVLPPHPHPHLSGEWSDFSDT 68

Query: 65  -TLSRKELEALLGLIGAEPPSEEELKTMMGAMDRVGPACHSELRDTFEIFDADHDGRITA 124
            T  RKELEALLGLIGAEPPSEEELK MM  +D+VGP CHSELRDTFEIFDADHDGRITA
Sbjct: 69  HTDYRKELEALLGLIGAEPPSEEELKMMMSQVDKVGPPCHSELRDTFEIFDADHDGRITA 128

Query: 125 EELFSVFTAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFARMMDCQR 165
           EELFSVF+AMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFA MM+CQR
Sbjct: 129 EELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFAVMMECQR 172

[Close]

BLAST of Bhi11G002433 vs. NCBI nr
Match: XP_022983878.1 (probable calcium-binding protein CML36 [Cucurbita maxima])

HSP 1 Score: 251.5 bits (641), Expect = 4.9e-63
Identity = 133/168 (79.17%), Postives = 143/168 (85.12%), Query Frame = 0


Query: 5   KSFNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSAGTPTSVLP-------SAVWSDFSDS 64
           KSF+P RLF SK HR+ LS+S+PPSFGSAS SS    PTSVLP       S  WSDFSD+
Sbjct: 21  KSFSPGRLFPSKKHRTSLSKSEPPSFGSASFSS----PTSVLPPHPHPHLSGEWSDFSDT 80

Query: 65  TLS-RKELEALLGLIGAEPPSEEELKTMMGAMDRVGPACHSELRDTFEIFDADHDGRITA 124
            +  RKELEALLGLIGAEPPSEEELK MM  +D+VGPACHSELRDTFEIFDADHDGRITA
Sbjct: 81  HIDYRKELEALLGLIGAEPPSEEELKMMMSQVDKVGPACHSELRDTFEIFDADHDGRITA 140

Query: 125 EELFSVFTAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFARMMDCQR 165
           EELFSVF+AMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFA MM+CQR
Sbjct: 141 EELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFAVMMECQR 184

[Close]

BLAST of Bhi11G002433 vs. ExPASy TrEMBL
Match: A0A5A7UEZ0 (Putative calcium-binding protein CML35 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007460 PE=4 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 1.3e-77
Identity = 154/168 (91.67%), Postives = 158/168 (94.05%), Query Frame = 0


Query: 1   MKLLKSFNPNRLFSSKSHRSKLSRSQPPSFGSASSS----SSAGTPTSVLPSAVWSDFSD 60
           MKLLKSFNPNRLFSSKSHRSKLSRSQPPSFGSASSS    SSAGTPTSVLPS  W+DFSD
Sbjct: 1   MKLLKSFNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSSTSSAGTPTSVLPSLDWADFSD 60

Query: 61  STLSRKELEALLGLIGAEPPSEEELKTMMGAMDRVGPACHSELRDTFEIFDADHDGRITA 120
            TLSRKEL+ALLGLIGAEPPSEEE+K MMG MDRVGPACHSELRDTFEIFDADHDGRITA
Sbjct: 61  PTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPACHSELRDTFEIFDADHDGRITA 120

Query: 121 EELFSVFTAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFARMMDCQR 165
           EELFSVFTAMGDE CTLEDCRRMIAGVDKNGDGFVCF+DFARMMDCQR
Sbjct: 121 EELFSVFTAMGDEGCTLEDCRRMIAGVDKNGDGFVCFDDFARMMDCQR 168

[Close]

BLAST of Bhi11G002433 vs. ExPASy TrEMBL
Match: A0A1S3B7R8 (probable calcium-binding protein CML35 OS=Cucumis melo OX=3656 GN=LOC103486950 PE=4 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 1.3e-77
Identity = 154/168 (91.67%), Postives = 158/168 (94.05%), Query Frame = 0


Query: 1   MKLLKSFNPNRLFSSKSHRSKLSRSQPPSFGSASSS----SSAGTPTSVLPSAVWSDFSD 60
           MKLLKSFNPNRLFSSKSHRSKLSRSQPPSFGSASSS    SSAGTPTSVLPS  W+DFSD
Sbjct: 1   MKLLKSFNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSSTSSAGTPTSVLPSLDWADFSD 60

Query: 61  STLSRKELEALLGLIGAEPPSEEELKTMMGAMDRVGPACHSELRDTFEIFDADHDGRITA 120
            TLSRKEL+ALLGLIGAEPPSEEE+K MMG MDRVGPACHSELRDTFEIFDADHDGRITA
Sbjct: 61  PTLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPACHSELRDTFEIFDADHDGRITA 120

Query: 121 EELFSVFTAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFARMMDCQR 165
           EELFSVFTAMGDE CTLEDCRRMIAGVDKNGDGFVCF+DFARMMDCQR
Sbjct: 121 EELFSVFTAMGDEGCTLEDCRRMIAGVDKNGDGFVCFDDFARMMDCQR 168

[Close]

BLAST of Bhi11G002433 vs. ExPASy TrEMBL
Match: A0A0A0LFY7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G823060 PE=4 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 4.6e-75
Identity = 149/167 (89.22%), Postives = 154/167 (92.22%), Query Frame = 0


Query: 1   MKLLKSFNPNRLFSSKSHRSKLSRSQPPSFGSASSSSS---AGTPTSVLPSAVWSDFSDS 60
           MKLLKS NPNRLFSSKSHRSKLSRSQPPSFGSASSSSS   AGTPTSVLPS  WSDFSD 
Sbjct: 1   MKLLKSLNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSSSAAGTPTSVLPSLDWSDFSDP 60

Query: 61  TLSRKELEALLGLIGAEPPSEEELKTMMGAMDRVGPACHSELRDTFEIFDADHDGRITAE 120
           TLSRKEL+ALLGLIGAEPPSEEE+K MMG MDRVGP CHSELRDTFEIFDADHDGRITAE
Sbjct: 61  TLSRKELKALLGLIGAEPPSEEEIKIMMGEMDRVGPTCHSELRDTFEIFDADHDGRITAE 120

Query: 121 ELFSVFTAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFARMMDCQR 165
           ELFSVF AMGD+ CTLEDC+RMIAGVDKNGDGFVCF+DF RMMDCQR
Sbjct: 121 ELFSVFAAMGDDGCTLEDCQRMIAGVDKNGDGFVCFDDFVRMMDCQR 167

[Close]

BLAST of Bhi11G002433 vs. ExPASy TrEMBL
Match: A0A6J1J8Y7 (probable calcium-binding protein CML36 OS=Cucurbita maxima OX=3661 GN=LOC111482360 PE=4 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 2.4e-63
Identity = 133/168 (79.17%), Postives = 143/168 (85.12%), Query Frame = 0


Query: 5   KSFNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSAGTPTSVLP-------SAVWSDFSDS 64
           KSF+P RLF SK HR+ LS+S+PPSFGSAS SS    PTSVLP       S  WSDFSD+
Sbjct: 21  KSFSPGRLFPSKKHRTSLSKSEPPSFGSASFSS----PTSVLPPHPHPHLSGEWSDFSDT 80

Query: 65  TLS-RKELEALLGLIGAEPPSEEELKTMMGAMDRVGPACHSELRDTFEIFDADHDGRITA 124
            +  RKELEALLGLIGAEPPSEEELK MM  +D+VGPACHSELRDTFEIFDADHDGRITA
Sbjct: 81  HIDYRKELEALLGLIGAEPPSEEELKMMMSQVDKVGPACHSELRDTFEIFDADHDGRITA 140

Query: 125 EELFSVFTAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFARMMDCQR 165
           EELFSVF+AMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFA MM+CQR
Sbjct: 141 EELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFAVMMECQR 184

[Close]

BLAST of Bhi11G002433 vs. ExPASy TrEMBL
Match: A0A6J1FB49 (probable calcium-binding protein CML36 OS=Cucurbita moschata OX=3662 GN=LOC111442449 PE=4 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 2.4e-63
Identity = 134/168 (79.76%), Postives = 143/168 (85.12%), Query Frame = 0


Query: 5   KSFNPNRLFSSKSHRSKLSRSQPPSFGSASSSSSAGTPTSVLP-------SAVWSDFSDS 64
           KSF+P RLF SK HR+ LS+S+PPSFGSASSSS    PTSVLP       S  WSDFSD+
Sbjct: 21  KSFSPGRLFPSKKHRTSLSKSEPPSFGSASSSS----PTSVLPPHPHPHLSGEWSDFSDT 80

Query: 65  -TLSRKELEALLGLIGAEPPSEEELKTMMGAMDRVGPACHSELRDTFEIFDADHDGRITA 124
            T  RKELEALLGLIGAEPPSEEELK MM  +D+VGP CHSELRDTFEIFDADHDGRITA
Sbjct: 81  HTDYRKELEALLGLIGAEPPSEEELKMMMSQVDKVGPPCHSELRDTFEIFDADHDGRITA 140

Query: 125 EELFSVFTAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFARMMDCQR 165
           EELFSVF+AMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFA MM+CQR
Sbjct: 141 EELFSVFSAMGDEQCTLEDCRRMIAGVDKNGDGFVCFEDFAVMMECQR 184

The following BLAST results are available for this feature:

BLAST of Bhi11G002433 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G10190.1	3.4e-30	44.55	Calcium-binding EF-hand family protein	[more]
AT2G41410.1	5.8e-30	44.50	Calcium-binding EF-hand family protein	[more]
AT1G24620.1	4.8e-16	38.71	EF hand calcium-binding protein family	[more]
AT1G66400.1	1.7e-13	34.33	calmodulin like 23	[more]
AT2G15680.1	3.8e-13	32.16	Calcium-binding EF-hand family protein	[more]

BLAST of Bhi11G002433 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SS31	4.8e-29	44.55	Probable calcium-binding protein CML36 OS=Arabidopsis thaliana OX=3702 GN=CML36 ...	[more]
P30188	8.2e-29	44.50	Probable calcium-binding protein CML35 OS=Arabidopsis thaliana OX=3702 GN=CML35 ...	[more]
Q9FYK2	6.8e-15	38.71	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ...	[more]
Q5ZCK5	8.3e-13	41.41	Probable calcium-binding protein CML16 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q8RZB5	2.4e-12	34.15	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of Bhi11G002433 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038903370.1	1.7e-84	100.00	probable calcium-binding protein CML36 [Benincasa hispida]	[more]
XP_008443339.2	2.7e-77	91.67	PREDICTED: probable calcium-binding protein CML35 [Cucumis melo] >KAA0053814.1 p...	[more]
XP_004136671.2	9.5e-75	89.22	probable calcium-binding protein CML35 [Cucumis sativus] >KGN59507.1 hypothetica...	[more]
KAG6580931.1	4.9e-63	79.76	putative calcium-binding protein CML36, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_022983878.1	4.9e-63	79.17	probable calcium-binding protein CML36 [Cucurbita maxima]	[more]

BLAST of Bhi11G002433 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7UEZ0	1.3e-77	91.67	Putative calcium-binding protein CML35 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3B7R8	1.3e-77	91.67	probable calcium-binding protein CML35 OS=Cucumis melo OX=3656 GN=LOC103486950 P...	[more]
A0A0A0LFY7	4.6e-75	89.22	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G823060 PE=4 SV=1	[more]
A0A6J1J8Y7	2.4e-63	79.17	probable calcium-binding protein CML36 OS=Cucurbita maxima OX=3661 GN=LOC1114823...	[more]
A0A6J1FB49	2.4e-63	79.76	probable calcium-binding protein CML36 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 135..163 e-value: 0.0022 score: 27.2 coord: 98..126 e-value: 2.7E-5 score: 33.6
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 98..160 e-value: 2.6E-9 score: 37.4
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 131..164 score: 11.695971
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 94..129 score: 13.09074
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 98..160 e-value: 2.36272E-18 score: 72.5805
None	No IPR available	GENE3D	1.10.238.10		coord: 94..164 e-value: 4.0E-20 score: 74.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..44
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 8..44
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 1..51 coord: 92..163
None	No IPR available	PANTHER	PTHR10891:SF869	SUBFAMILY NOT NAMED	coord: 1..51 coord: 92..163
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 144..156
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 107..119
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 53..160

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi11M002433	Bhi11M002433	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding