Bhi11G002301 (gene) Wax gourd (B227) v1

Overview
NameBhi11G002301
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptionhaloacid dehalogenase-like hydrolase domain-containing protein 3
Locationchr11: 74263683 .. 74266899 (+)
RNA-Seq ExpressionBhi11G002301
SyntenyBhi11G002301
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAATCAGAATCAAGGGGTATTATCGGTAATAAGAATAAAGTTAAAGAGTAAATTGGGGAATACCGTATAAGAAGGATCATAATTTCCTTCTTTGGCAGGCTTCTATTTAAAGCCACTCGAAATAACTCTCAAAAACATTTTCCATTTTACAGCCGAAGGAATAGCGAAGAAGAAGAGAAAGAGAGTTCTGTGAAAAGCTTGCACTCAAATGAAGTTCGCTAATGGCGCTAATTGCTACTAGAACCAAGATCTCGAGGATTCTCTCCTCTTTCTCTCCTAAGACCTTGCTTTCTAGACCTCCATTTTCCTCTAATGGCGTCCAGGCGGCTGCTCGGCTCCACCCGCTGGGAGGATTCGGGCCTTCATTTGCATCCTCTGCTGTTGCTTCCATGGAGCCCGAAGCCCTAGGTAACAGAGCTATTGGCTTTGAAATCTTGGGCGTTAAGGATTATGAAGACTATCGGAGGTCTCTCTATGGCGAAATCACTCACAAGGCTTTGCTCGTTGATGCCGTTGGTACTCTTGTGGTTCCTTCTCAACCTATGGCTCAGGTCTCCCTCTCTTTGTCCCTCTCTCTTCAATTTTGTTTTCAAGTTTCGATTTTATGAAGTGAATCATTCGTTTTATGGTTACTATAGTTTATTTTTGTGGATCTTGTTGCAGATATATAGGGAAATAGGTGAGAAGTATGGAGTGAACTACTCGGAAGGGGAGATTCTGAATAGATACAGAAGGGCGTACGAGAAGCCTTGGGGTAGATCTCGTCTCAGGTCGACTTTTCTTTTTCTTTTGGAGCGTCTTCCAATTTTAGTTGACTATATTATCCTTTATTAATGTTGTCGTCTGGATCTCGCAGACCTTGTGTTCTGTGAAAGAAAAGTCTTGTCTAAAAGACCGTTTAATTGTTGACTAGATTAACTTGAATGAATAATTAAGAAGAATTAATTTATATTGTCTTTTAGGTGAATAGCTGTTCATCAAAGTGCATGCATGTGCTATTTGGGTTTGTAGTTTAGTATAGAATTATTTGGCTTAATGAGTATGGTTTCAAGGAAATTATGGTGTATTTAATGAATTTTTTTCAACAGTTAGGTTACTGACCATTATAATTCTAGCTTTATGCTTGTGGCTACCCAATATCCTAGTCAGTTTTGTAAAGTGCTTTGGTCAGAATTAAGTGGACTCAAACCCTTAATAAACATTGATCAGAATTGATTGTCATGGCCTGTGTTTTCTTGGTCAGCCCGAAGATTAAATTGGTCTAAATATCTTTAATAACTGTAAATCAGCCTTGCCTTAGAAAGTTAGAAACACAACCTTTTTTCTTCTGTTCTTTTTTATTGGAGCAATTGCTTGCTCGTTAATGATCTAATGAGATCCTTGTTACTATGTGCATACCACTAAGGTCCCATTAATTTTTCAAAATTAAACTTATTTCTCTCAATTTCTTACTGTAGTTTTCATCTTTGAGTAAAACGATTGAATTCTTAACCAAATTTCATAAATAAAAAACTATTTTTTCTTAAAAAGTAGATACCAAAACAAGAAATTTAGAGGTAAAAGGAGTGTTGGCTTGATTTTAAAAAACCTAAAATCTAATTGGTTACTAAATGAACTAAAGATTTTGTTTTGTTCGGAGATTAAGAAACCTTTTCCCGAAAAAAAAGGGCTCTTTGTATTGCAGTTGTATCTGCAATTTGAAAGGTAGAATATCAACTAAATACCATGAGGGTAAAAAAGGGCTTTTGTTTATGTGGTTATCTTCCTATCTTTAATCCAAACTTGATTCCTTAATCCATTAATTGATCTTTTGAGTACTGTCTTGTCTGACTAATCCAGGGACAGCGAGAAGTTTTTTAATTCTTCTCCAATTCCTTTCCTTAATGAAAGTAATTGCTTTTATGGAACTCTGCAGATATGTGAGTGATGGGAAACCCTTTTGGCAATATATCGTCAGTTCTTCCACGGGCTGTTCTGATTCTCAGTATTTTGAAGAACTCTACAACTACTATACGACTAAGAAGGTTAGAGTTTTTGGCAGCCGCTTTTTTCTCTGGCAAATCTACTGTTTTGTGATGTCTTTTATTATTATTGTAACGATCTTTTTTGCTTATAAATCTCTTGCAGGCCTGGCACCTTTGTGATCCTGATGCTGAGAGAGTCTTTAAAGCTCTCAGACAAGCAGGTGTAAAGATAGCCGTTGTGTCAAACTTCGACACTCGATTAAGACCTCTATTGCGGGCTTTGAATTGCGATCACTGGTTTGACGCTGTAGCAGTGTCAGCTGAAGTGAGTTATTCATCCATTTTCATGTTTTTTGCTGCTTGCTCTTCTCTTTTTCATGTGTATTTCATGAGCTAGGTTTAATAATCTTGGTTACAAACTGCTTTACTTTGCCTTTGACATGTACATGTGAACTTACATTCAGTTGTTCTTCCTAACCTGATCTCGAACAGGTTGAAGCCGAGAAACCTAATCCGACTATATTCCTAAAAGCCTGTGAATTGTTGGGAGTGAGCCCTGAGGATGCCTTACATGTTGGGGATGATCGTAGGAACGATATATGGGGCGCTAGAGATGCCGGTTGTGATGCATGGCTTTGGGGAAGTGATGTACACTCCTTCAAAGAGGTATGCTTCAATCCATAAATCACGCTACCATGTCATGTAGTTTACTTCCATTTATCTGTCTCAAATCGATTCTTCGTCGTTTTTCGATCACAAAGTTGACAGCCTGCTGGTATTTTCTTTTGGTGATGTAGGTTGCAGAGAGGATTGGAGTGAAAGTGTGAAACGGGGGTTATGACTTAGGTAAAAGCATTAAACTTGTTGGATTTCCATGGAAGCTCTCTGGATGTACAGGGTTTTTACCAGTGAGAGCAGCTGAGTGTGTATATTTTGTGTGCTGTGTTTGTATAGTTAGTCCAACTGTTTTTGCTACCAAGCAAGCACACTAAATAAAAAAGAACTCTTTTTCTTCTTGTTTGTGTCCATAAAGTGGTAAATTGCAGATCTTAGTGGTAAAAAGAGTGTATGGGTTTAATAAAAGAAATTAATCTATGGTGGCCATTTAGTTAGGATTAATATCTTATAAGTTTCTTTCAGATTCAAATGTTGTAGCGTTAAGTAATTTGTCAATTAAGATTAATAGAGATGTGCATTACTTGGACCCAGATGTGCTATTAATAATATTTAATAAGTAAGTACGACATGGAAAA

mRNA sequence

GAATCAGAATCAAGGGGTATTATCGGTAATAAGAATAAAGTTAAAGAGTAAATTGGGGAATACCGTATAAGAAGGATCATAATTTCCTTCTTTGGCAGGCTTCTATTTAAAGCCACTCGAAATAACTCTCAAAAACATTTTCCATTTTACAGCCGAAGGAATAGCGAAGAAGAAGAGAAAGAGAGTTCTGTGAAAAGCTTGCACTCAAATGAAGTTCGCTAATGGCGCTAATTGCTACTAGAACCAAGATCTCGAGGATTCTCTCCTCTTTCTCTCCTAAGACCTTGCTTTCTAGACCTCCATTTTCCTCTAATGGCGTCCAGGCGGCTGCTCGGCTCCACCCGCTGGGAGGATTCGGGCCTTCATTTGCATCCTCTGCTGTTGCTTCCATGGAGCCCGAAGCCCTAGGTAACAGAGCTATTGGCTTTGAAATCTTGGGCGTTAAGGATTATGAAGACTATCGGAGGTCTCTCTATGGCGAAATCACTCACAAGGCTTTGCTCGTTGATGCCGTTGGTACTCTTGTGGTTCCTTCTCAACCTATGGCTCAGATATATAGGGAAATAGGTGAGAAGTATGGAGTGAACTACTCGGAAGGGGAGATTCTGAATAGATACAGAAGGGCGTACGAGAAGCCTTGGGGTAGATCTCGTCTCAGATATGTGAGTGATGGGAAACCCTTTTGGCAATATATCGTCAGTTCTTCCACGGGCTGTTCTGATTCTCAGTATTTTGAAGAACTCTACAACTACTATACGACTAAGAAGGCCTGGCACCTTTGTGATCCTGATGCTGAGAGAGTCTTTAAAGCTCTCAGACAAGCAGGTGTAAAGATAGCCGTTGTGTCAAACTTCGACACTCGATTAAGACCTCTATTGCGGGCTTTGAATTGCGATCACTGGTTTGACGCTGTAGCAGTGTCAGCTGAAGTTGAAGCCGAGAAACCTAATCCGACTATATTCCTAAAAGCCTGTGAATTGTTGGGAGTGAGCCCTGAGGATGCCTTACATGTTGGGGATGATCGTAGGAACGATATATGGGGCGCTAGAGATGCCGGTTGTGATGCATGGCTTTGGGGAAGTGATGTACACTCCTTCAAAGAGGTTGCAGAGAGGATTGGAGTGAAAGTGTGAAACGGGGGTTATGACTTAGGTAAAAGCATTAAACTTGTTGGATTTCCATGGAAGCTCTCTGGATGTACAGGGTTTTTACCAGTGAGAGCAGCTGAGTGTGTATATTTTGTGTGCTGTGTTTGTATAGTTAGTCCAACTGTTTTTGCTACCAAGCAAGCACACTAAATAAAAAAGAACTCTTTTTCTTCTTGTTTGTGTCCATAAAGTGGTAAATTGCAGATCTTAGTGGTAAAAAGAGTGTATGGGTTTAATAAAAGAAATTAATCTATGGTGGCCATTTAGTTAGGATTAATATCTTATAAGTTTCTTTCAGATTCAAATGTTGTAGCGTTAAGTAATTTGTCAATTAAGATTAATAGAGATGTGCATTACTTGGACCCAGATGTGCTATTAATAATATTTAATAAGTAAGTACGACATGGAAAA

Coding sequence (CDS)

ATGGCGCTAATTGCTACTAGAACCAAGATCTCGAGGATTCTCTCCTCTTTCTCTCCTAAGACCTTGCTTTCTAGACCTCCATTTTCCTCTAATGGCGTCCAGGCGGCTGCTCGGCTCCACCCGCTGGGAGGATTCGGGCCTTCATTTGCATCCTCTGCTGTTGCTTCCATGGAGCCCGAAGCCCTAGGTAACAGAGCTATTGGCTTTGAAATCTTGGGCGTTAAGGATTATGAAGACTATCGGAGGTCTCTCTATGGCGAAATCACTCACAAGGCTTTGCTCGTTGATGCCGTTGGTACTCTTGTGGTTCCTTCTCAACCTATGGCTCAGATATATAGGGAAATAGGTGAGAAGTATGGAGTGAACTACTCGGAAGGGGAGATTCTGAATAGATACAGAAGGGCGTACGAGAAGCCTTGGGGTAGATCTCGTCTCAGATATGTGAGTGATGGGAAACCCTTTTGGCAATATATCGTCAGTTCTTCCACGGGCTGTTCTGATTCTCAGTATTTTGAAGAACTCTACAACTACTATACGACTAAGAAGGCCTGGCACCTTTGTGATCCTGATGCTGAGAGAGTCTTTAAAGCTCTCAGACAAGCAGGTGTAAAGATAGCCGTTGTGTCAAACTTCGACACTCGATTAAGACCTCTATTGCGGGCTTTGAATTGCGATCACTGGTTTGACGCTGTAGCAGTGTCAGCTGAAGTTGAAGCCGAGAAACCTAATCCGACTATATTCCTAAAAGCCTGTGAATTGTTGGGAGTGAGCCCTGAGGATGCCTTACATGTTGGGGATGATCGTAGGAACGATATATGGGGCGCTAGAGATGCCGGTTGTGATGCATGGCTTTGGGGAAGTGATGTACACTCCTTCAAAGAGGTTGCAGAGAGGATTGGAGTGAAAGTGTGA

Protein sequence

MALIATRTKISRILSSFSPKTLLSRPPFSSNGVQAAARLHPLGGFGPSFASSAVASMEPEALGNRAIGFEILGVKDYEDYRRSLYGEITHKALLVDAVGTLVVPSQPMAQIYREIGEKYGVNYSEGEILNRYRRAYEKPWGRSRLRYVSDGKPFWQYIVSSSTGCSDSQYFEELYNYYTTKKAWHLCDPDAERVFKALRQAGVKIAVVSNFDTRLRPLLRALNCDHWFDAVAVSAEVEAEKPNPTIFLKACELLGVSPEDALHVGDDRRNDIWGARDAGCDAWLWGSDVHSFKEVAERIGVKV
Homology
BLAST of Bhi11G002301 vs. TAIR 10
Match: AT2G41250.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 420.2 bits (1079), Expect = 1.4e-117
Identity = 195/256 (76.17%), Postives = 227/256 (88.67%), Query Frame = 0

Query: 48  SFASSAVASMEPEALGNRAIGFEILGVKDYEDYRRSLYGEITHKALLVDAVGTLVVPSQP 107
           S +SS+    + E+ G   +G  +   K+YEDYRRSLYGEITHKALLVDAVGTL+VP+QP
Sbjct: 38  SASSSSAVVADDESSGIYGLGSVL---KEYEDYRRSLYGEITHKALLVDAVGTLLVPAQP 97

Query: 108 MAQIYREIGEKYGVNYSEGEILNRYRRAYEKPWGRSRLRYVSDGKPFWQYIVSSSTGCSD 167
            AQIY+ IGEKYGV YSE EIL RYRRAY+KPWG S LRYV+D +PFWQYIV++STGCSD
Sbjct: 98  TAQIYKNIGEKYGVEYSEAEILTRYRRAYQKPWGGSHLRYVNDARPFWQYIVTASTGCSD 157

Query: 168 SQYFEELYNYYTTKKAWHLCDPDAERVFKALRQAGVKIAVVSNFDTRLRPLLRALNCDHW 227
           SQYFEELY+Y+TT++AW LCDPDA +VFKA+++AGVK+A+VSNFDTRLRPLLRAL C+ W
Sbjct: 158 SQYFEELYSYFTTEQAWKLCDPDAGKVFKAIKEAGVKVAIVSNFDTRLRPLLRALRCEDW 217

Query: 228 FDAVAVSAEVEAEKPNPTIFLKACELLGVSPEDALHVGDDRRNDIWGARDAGCDAWLWGS 287
           FDAVAVSAEVEAEKPNPTIFLKACELL V+PEDA+HVGDDRRND+WGARDAGCDAWLWGS
Sbjct: 218 FDAVAVSAEVEAEKPNPTIFLKACELLEVNPEDAVHVGDDRRNDVWGARDAGCDAWLWGS 277

Query: 288 DVHSFKEVAERIGVKV 304
           +V SFK+VA+RIGVKV
Sbjct: 278 EVTSFKQVAQRIGVKV 290

BLAST of Bhi11G002301 vs. TAIR 10
Match: AT1G14310.1 (Haloacid dehalogenase-like hydrolase (HAD) superfamily protein )

HSP 1 Score: 205.7 bits (522), Expect = 5.3e-53
Identity = 101/210 (48.10%), Postives = 134/210 (63.81%), Query Frame = 0

Query: 90  HKALLVDAVGTLVVPSQPMAQIYREIGEKYGVNYSEGEILNRYRRAYEKPWGRSRLRYVS 149
           +  LL+DA GTL+  S+P+ + Y  +G+KYG+  +  EI   ++R +  PW   +LRY  
Sbjct: 42  YDGLLLDAGGTLLQLSKPVHETYASLGQKYGLKTTPAEIKEGFKRVFSAPW-PEKLRYQG 101

Query: 150 DGKPFWQYIVSSSTGCSDSQYFEELYNYYTTKKAWHLCDPDAERVFKALRQAGVKIAVVS 209
           DG+PFW+ +VS +TGCSD+ YFE++Y YY   +AWHL +  A      L+ AGVK+AVVS
Sbjct: 102 DGRPFWKLVVSEATGCSDNDYFEDVYQYYANGEAWHLPE-GAYETMSLLKDAGVKMAVVS 161

Query: 210 NFDTRLRPLLRALNCDHWFDAVAVSAEVEAEKPNPTIFLKACELLGVSPEDALHVGDDRR 269
           NFDTRLR LL+ LN    FDAV VSAEV  EKP+  IF  A E + V    A+HVGDD  
Sbjct: 162 NFDTRLRKLLKDLNVIDMFDAVIVSAEVGYEKPDERIFKSALEQISVDVNRAVHVGDDEG 221

Query: 270 NDIWGARDAGCDAWLWGSDVHSFKEVAERI 300
            D  GA   G   WLWG DV +F ++ +RI
Sbjct: 222 ADKGGANAIGIACWLWGEDVQTFSDIQKRI 249

BLAST of Bhi11G002301 vs. ExPASy Swiss-Prot
Match: Q5HZL9 (Haloacid dehalogenase-like hydrolase domain-containing protein 3 OS=Xenopus laevis OX=8355 GN=hdhd3 PE=2 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.7e-16
Identity = 61/199 (30.65%), Postives = 91/199 (45.73%), Query Frame = 0

Query: 96  DAVGTLVVPSQPMAQIYREIGEKYGVNYSEGEILNRYRRAY---EKPWGRSRLRYVSDGK 155
           D   TL+    P+ Q Y    +K G+  + G +   +R AY    + +    L      +
Sbjct: 9   DVKDTLLRVRVPVGQQYYAEAKKRGLCVNPGTLETSFRNAYRSHSRLFPNYGLAQGMSSR 68

Query: 156 PFWQYIVSSS---TGCSDSQYFE----ELYNYYTTKKAWHLCDPDAERVFKALRQAGVKI 215
            +W  +V  +   +G  DS   +    +LY  ++T   W L  P A     +    G+++
Sbjct: 69  QWWLDVVLQTFRLSGIEDSDTVQSLAKQLYQDFSTAHNWALV-PGAREALDSCTNLGLRM 128

Query: 216 AVVSNFDTRLRPLLRALNCDHWFDAVAVSAEVEAEKPNPTIFLKACELLGVSPEDALHVG 275
           AV+SNFD RL  LLR    + +FD V  +      KP+  IF KA  L  V P  A+HVG
Sbjct: 129 AVISNFDRRLEELLRQCCLERYFDFVVTAESAGVAKPHLGIFHKALSLAKVPPHQAVHVG 188

Query: 276 DDRRNDIWGARDAGCDAWL 285
           DD  ND   AR  G  ++L
Sbjct: 189 DDYVNDYCAARMVGMHSYL 206

BLAST of Bhi11G002301 vs. ExPASy Swiss-Prot
Match: Q9CYW4 (Haloacid dehalogenase-like hydrolase domain-containing protein 3 OS=Mus musculus OX=10090 GN=Hdhd3 PE=1 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 1.5e-15
Identity = 61/207 (29.47%), Postives = 96/207 (46.38%), Query Frame = 0

Query: 91  KALLVDAVGTLVVPSQPMAQIYREIGEKYGVNYSEGEILNRYRRAYEKP------WGRSR 150
           + L  D   TL+   +P+ + Y      +GV   +  +   +R+AY         +G SR
Sbjct: 8   RLLTWDVKDTLIKLRRPVGEEYASKARAHGVVVEDITVEQAFRQAYRAQSHNFPNYGLSR 67

Query: 151 LRYVSDGKPFWQYIVSSS---TGCSDSQYF----EELYNYYTTKKAWHLCDPDAERVFKA 210
                  + +W+ +V  +    G  D+Q      ++LY  +++   W + +  AE   K 
Sbjct: 68  ---GLTSRQWWKDVVLHTFRLAGVPDAQAMTPVADQLYEDFSSPFTWQVLE-GAEMTLKG 127

Query: 211 LRQAGVKIAVVSNFDTRLRPLLRALNCDHWFDAVAVSAEVEAEKPNPTIFLKACELLGVS 270
            R+ G+K+AVVSNFD RL  +L  L     FD V  S  V   KP+P IF +A +   V 
Sbjct: 128 CRKRGLKLAVVSNFDRRLEDILTGLGLREHFDFVLTSEAVGCPKPDPRIFREALQRACVE 187

Query: 271 PEDALHVGDDRRNDIWGARDAGCDAWL 285
           P  A HVGD    D  G++  G  ++L
Sbjct: 188 PAVAAHVGDSYLCDYQGSQAVGMHSFL 210

BLAST of Bhi11G002301 vs. ExPASy Swiss-Prot
Match: Q94915 (Rhythmically expressed gene 2 protein OS=Drosophila melanogaster OX=7227 GN=Reg-2 PE=2 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 3.3e-15
Identity = 59/209 (28.23%), Postives = 95/209 (45.45%), Query Frame = 0

Query: 91  KALLVDAVGTLVVPSQPMAQIYREIGEKYGVNYSEGEILNRYRRAYEKPWGRSRLRYVSD 150
           + +  D   TL+       + Y EIG  +G      E+   ++  + K   R    +  D
Sbjct: 8   RLITFDVTNTLLQFRTTPGKQYGEIGALFGARCDNNELAKNFKANWYK-MNRDYPNFGRD 67

Query: 151 GKP------FWQYIVSSSTGCSDSQY-FEELYNY-------YTTKKAWHLCDPDAE---R 210
             P      +W+ +++ +   S +    E+L+N+       Y T   W  C+   E   +
Sbjct: 68  TNPQMEWQQWWRKLIAGTFAESGAAIPDEKLHNFSNHLIELYKTSICWQPCNGSVELLQQ 127

Query: 211 VFKALRQAGVKIAVVSNFDTRLRPLLRALNCDHWFDAVAVSAEVEAEKPNPTIFLKACE- 270
           + K L+    K+ V++NFD RL  LL+    D + D    S EV+AEKP+P IF KA E 
Sbjct: 128 LRKELKPEKCKLGVIANFDPRLPTLLQNTKLDQYLDFAINSYEVQAEKPDPQIFQKAMEK 187

Query: 271 --LLGVSPEDALHVGDDRRNDIWGARDAG 280
             L  + PE+ LH+GD    D   A++ G
Sbjct: 188 SGLKNLKPEECLHIGDGPTTDYLAAKELG 215

BLAST of Bhi11G002301 vs. ExPASy Swiss-Prot
Match: O14262 (Putative uncharacterized hydrolase C7D4.05 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC7D4.05 PE=3 SV=3)

HSP 1 Score: 84.0 bits (206), Expect = 3.3e-15
Identity = 56/197 (28.43%), Postives = 100/197 (50.76%), Query Frame = 0

Query: 91  KALLVDAVGTLVVPSQPMAQIYREIGEKYGVNYSEGEILNRYRRAYEKPWGRSRLRYVSD 150
           K +  DA GT++  S+P+  +Y E+ +KYGV+ +  EI +   +A++    + +      
Sbjct: 11  KLVTFDAFGTILHLSKPVPIVYSEVAQKYGVHATIDEIEHNSNKAFKDFSEKHKNHGKKS 70

Query: 151 G---KPFWQYIVSSSTGCS-DSQYFEELYNYYTTKKAWHLCDPDAERVFKALRQAGVKIA 210
           G     +W  ++  S      ++  EEL++Y++ K  + +     + + +   +    I 
Sbjct: 71  GLNPHDWWIKVIEHSFPTPVPAEMAEELWSYFSKKTGYTIHPLLIDFLKRNKEERKYIIG 130

Query: 211 VVSNFDTRLRPLLRALNCDHWFDAVAVSAEVEAEKPNPTIF----LKACELLG--VSPED 270
           ++SN D R+R +L     DH  D  A S +V  EKP+  IF     KA +LLG  + PE+
Sbjct: 131 IISNTDERIRTVLEDYGIDHLIDIYAFSYDVGFEKPSREIFDYAMEKAVKLLGQEIQPEE 190

Query: 271 ALHVGDDRRNDIWGARD 278
            +H+GDD   D+  AR+
Sbjct: 191 CMHLGDDLIKDVSAARN 207

BLAST of Bhi11G002301 vs. ExPASy Swiss-Prot
Match: Q5E9D6 (Haloacid dehalogenase-like hydrolase domain-containing protein 3 OS=Bos taurus OX=9913 GN=HDHD3 PE=2 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 2.8e-14
Identity = 56/204 (27.45%), Postives = 91/204 (44.61%), Query Frame = 0

Query: 91  KALLVDAVGTLVVPSQPMAQIYREIGEKYGVNYSEGEILNRYRRAYE---KPWGRSRLRY 150
           + L  D   TL+    P+   Y      +G+      +   +++AY+   + +    L +
Sbjct: 8   RLLTWDVKDTLLRLRHPVGVEYATKARAHGLEVEATALGQAFKQAYKAQSQSFPNYGLGH 67

Query: 151 VSDGKPFWQYIVSSS---TGCSDSQ----YFEELYNYYTTKKAWHLCDPDAERVFKALRQ 210
                 +W  +V  +    G  D+Q      E+LY  +++   W + +  AE   +  R+
Sbjct: 68  GLTSHQWWLDLVQQTFHQAGVRDAQAVAPIAEQLYKDFSSPSTWQVLE-GAEATLRGCRK 127

Query: 211 AGVKIAVVSNFDTRLRPLLRALNCDHWFDAVAVSAEVEAEKPNPTIFLKACELLGVSPED 270
            G+K+AVVSNFD RL  +L  +     FD V  S      KP+P IF +A  L  V P  
Sbjct: 128 RGLKLAVVSNFDRRLEDILEGVGLREHFDFVLTSEAAGWPKPDPRIFHEALHLAQVEPAV 187

Query: 271 ALHVGDDRRNDIWGARDAGCDAWL 285
             H+GD  + D  GAR  G  ++L
Sbjct: 188 GAHIGDSYQRDYKGARAVGMHSFL 210

BLAST of Bhi11G002301 vs. NCBI nr
Match: XP_038905682.1 (haloacid dehalogenase-like hydrolase domain-containing protein 3 [Benincasa hispida])

HSP 1 Score: 620.9 bits (1600), Expect = 5.7e-174
Identity = 303/303 (100.00%), Postives = 303/303 (100.00%), Query Frame = 0

Query: 1   MALIATRTKISRILSSFSPKTLLSRPPFSSNGVQAAARLHPLGGFGPSFASSAVASMEPE 60
           MALIATRTKISRILSSFSPKTLLSRPPFSSNGVQAAARLHPLGGFGPSFASSAVASMEPE
Sbjct: 1   MALIATRTKISRILSSFSPKTLLSRPPFSSNGVQAAARLHPLGGFGPSFASSAVASMEPE 60

Query: 61  ALGNRAIGFEILGVKDYEDYRRSLYGEITHKALLVDAVGTLVVPSQPMAQIYREIGEKYG 120
           ALGNRAIGFEILGVKDYEDYRRSLYGEITHKALLVDAVGTLVVPSQPMAQIYREIGEKYG
Sbjct: 61  ALGNRAIGFEILGVKDYEDYRRSLYGEITHKALLVDAVGTLVVPSQPMAQIYREIGEKYG 120

Query: 121 VNYSEGEILNRYRRAYEKPWGRSRLRYVSDGKPFWQYIVSSSTGCSDSQYFEELYNYYTT 180
           VNYSEGEILNRYRRAYEKPWGRSRLRYVSDGKPFWQYIVSSSTGCSDSQYFEELYNYYTT
Sbjct: 121 VNYSEGEILNRYRRAYEKPWGRSRLRYVSDGKPFWQYIVSSSTGCSDSQYFEELYNYYTT 180

Query: 181 KKAWHLCDPDAERVFKALRQAGVKIAVVSNFDTRLRPLLRALNCDHWFDAVAVSAEVEAE 240
           KKAWHLCDPDAERVFKALRQAGVKIAVVSNFDTRLRPLLRALNCDHWFDAVAVSAEVEAE
Sbjct: 181 KKAWHLCDPDAERVFKALRQAGVKIAVVSNFDTRLRPLLRALNCDHWFDAVAVSAEVEAE 240

Query: 241 KPNPTIFLKACELLGVSPEDALHVGDDRRNDIWGARDAGCDAWLWGSDVHSFKEVAERIG 300
           KPNPTIFLKACELLGVSPEDALHVGDDRRNDIWGARDAGCDAWLWGSDVHSFKEVAERIG
Sbjct: 241 KPNPTIFLKACELLGVSPEDALHVGDDRRNDIWGARDAGCDAWLWGSDVHSFKEVAERIG 300

Query: 301 VKV 304
           VKV
Sbjct: 301 VKV 303

BLAST of Bhi11G002301 vs. NCBI nr
Match: XP_011652195.1 (haloacid dehalogenase-like hydrolase domain-containing protein 3 [Cucumis sativus] >KGN59453.1 hypothetical protein Csa_001198 [Cucumis sativus])

HSP 1 Score: 586.6 bits (1511), Expect = 1.2e-163
Identity = 283/303 (93.40%), Postives = 292/303 (96.37%), Query Frame = 0

Query: 1   MALIATRTKISRILSSFSPKTLLSRPPFSSNGVQAAARLHPLGGFGPSFASSAVASMEPE 60
           MALI+TRTKISRILS+FSP TLLSRPPF SNGVQAAARL P GGFG SFASSAVAS+EPE
Sbjct: 1   MALISTRTKISRILSTFSPNTLLSRPPFPSNGVQAAARLLPFGGFGSSFASSAVASVEPE 60

Query: 61  ALGNRAIGFEILGVKDYEDYRRSLYGEITHKALLVDAVGTLVVPSQPMAQIYREIGEKYG 120
            LG+  IGFEILGVKDYEDYRRSLYG+ITHKALLVDAVGTLVVPSQPMAQIYREIGEKYG
Sbjct: 61  TLGSSGIGFEILGVKDYEDYRRSLYGDITHKALLVDAVGTLVVPSQPMAQIYREIGEKYG 120

Query: 121 VNYSEGEILNRYRRAYEKPWGRSRLRYVSDGKPFWQYIVSSSTGCSDSQYFEELYNYYTT 180
           VNYSEGEILNRYRRAYEKPWGRSRLRYV+DGKPFWQYIVSSSTGCSDSQYFEELYNYYTT
Sbjct: 121 VNYSEGEILNRYRRAYEKPWGRSRLRYVNDGKPFWQYIVSSSTGCSDSQYFEELYNYYTT 180

Query: 181 KKAWHLCDPDAERVFKALRQAGVKIAVVSNFDTRLRPLLRALNCDHWFDAVAVSAEVEAE 240
            KAWHLCDPDAE+VFKALRQAGVKIA+VSNFDTRLRPLLR LNCDHWFDAVAVSAEVEAE
Sbjct: 181 NKAWHLCDPDAEKVFKALRQAGVKIAIVSNFDTRLRPLLRDLNCDHWFDAVAVSAEVEAE 240

Query: 241 KPNPTIFLKACELLGVSPEDALHVGDDRRNDIWGARDAGCDAWLWGSDVHSFKEVAERIG 300
           KPNPTIFLKACELLGV PEDALHVGDDRRND+WGARDAGCDAWLWGSDVHSFKEVAERIG
Sbjct: 241 KPNPTIFLKACELLGVRPEDALHVGDDRRNDVWGARDAGCDAWLWGSDVHSFKEVAERIG 300

Query: 301 VKV 304
           VKV
Sbjct: 301 VKV 303

BLAST of Bhi11G002301 vs. NCBI nr
Match: XP_008443269.1 (PREDICTED: haloacid dehalogenase-like hydrolase domain-containing protein 3 [Cucumis melo] >KAA0053875.1 haloacid dehalogenase-like hydrolase domain-containing protein 3 [Cucumis melo var. makuwa] >TYK25529.1 haloacid dehalogenase-like hydrolase domain-containing protein 3 [Cucumis melo var. makuwa])

HSP 1 Score: 584.7 bits (1506), Expect = 4.5e-163
Identity = 282/303 (93.07%), Postives = 290/303 (95.71%), Query Frame = 0

Query: 1   MALIATRTKISRILSSFSPKTLLSRPPFSSNGVQAAARLHPLGGFGPSFASSAVASMEPE 60
           MALIA RTKISR LS+FS  +LLSRPPF SNGVQA ARLHP GG G SF+SSAVASMEPE
Sbjct: 1   MALIAARTKISRTLSTFSLNSLLSRPPFPSNGVQAVARLHPFGGLGSSFSSSAVASMEPE 60

Query: 61  ALGNRAIGFEILGVKDYEDYRRSLYGEITHKALLVDAVGTLVVPSQPMAQIYREIGEKYG 120
            LG+R IGFEILGVKDYEDYRRSLYG+ITHKALLVDAVGTLVVPSQPMAQIYREIGEKYG
Sbjct: 61  TLGSRGIGFEILGVKDYEDYRRSLYGDITHKALLVDAVGTLVVPSQPMAQIYREIGEKYG 120

Query: 121 VNYSEGEILNRYRRAYEKPWGRSRLRYVSDGKPFWQYIVSSSTGCSDSQYFEELYNYYTT 180
           VNYSEGEILNRYRRAYEKPWGRSRLRYV+DGKPFWQYIVSSSTGCSDSQYFEELYNYYTT
Sbjct: 121 VNYSEGEILNRYRRAYEKPWGRSRLRYVNDGKPFWQYIVSSSTGCSDSQYFEELYNYYTT 180

Query: 181 KKAWHLCDPDAERVFKALRQAGVKIAVVSNFDTRLRPLLRALNCDHWFDAVAVSAEVEAE 240
            KAWHLCDPDAERVFKALRQAGVKIA+VSNFDTRLRPLLRALNCDHWFDAVAVSAEVEAE
Sbjct: 181 NKAWHLCDPDAERVFKALRQAGVKIAIVSNFDTRLRPLLRALNCDHWFDAVAVSAEVEAE 240

Query: 241 KPNPTIFLKACELLGVSPEDALHVGDDRRNDIWGARDAGCDAWLWGSDVHSFKEVAERIG 300
           KPNPTIFLKACELLGV PEDALHVGDDRRND+WGARDAGCDAWLWGSDVHSFKEVAERIG
Sbjct: 241 KPNPTIFLKACELLGVRPEDALHVGDDRRNDLWGARDAGCDAWLWGSDVHSFKEVAERIG 300

Query: 301 VKV 304
           VKV
Sbjct: 301 VKV 303

BLAST of Bhi11G002301 vs. NCBI nr
Match: XP_022935563.1 (haloacid dehalogenase-like hydrolase domain-containing protein 3 [Cucurbita moschata] >KAG6580886.1 Rhythmically expressed gene 2 protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017644.1 Rhythmically expressed gene 2 protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 572.0 bits (1473), Expect = 3.0e-159
Identity = 277/303 (91.42%), Postives = 287/303 (94.72%), Query Frame = 0

Query: 1   MALIATRTKISRILSSFSPKTLLSRPPFSSNGVQAAARLHPLGGFGPSFASSAVASMEPE 60
           MALI+ RTKISR+LS+ S K  LSR PF SNGVQ   RLHPLGGFG SFASSAVAS+E +
Sbjct: 1   MALISARTKISRVLSTLSHKNFLSRSPFFSNGVQPVDRLHPLGGFGRSFASSAVASVEHD 60

Query: 61  ALGNRAIGFEILGVKDYEDYRRSLYGEITHKALLVDAVGTLVVPSQPMAQIYREIGEKYG 120
           ALG+RA+GFEILGVKDYEDYRRSLYGEITHKALLVDAVGTLVVPSQPMAQIYREIGEKYG
Sbjct: 61  ALGSRAMGFEILGVKDYEDYRRSLYGEITHKALLVDAVGTLVVPSQPMAQIYREIGEKYG 120

Query: 121 VNYSEGEILNRYRRAYEKPWGRSRLRYVSDGKPFWQYIVSSSTGCSDSQYFEELYNYYTT 180
           VNYSEGEILNRYRRAYEKPWG SRLRYVSDGKPFWQYIVSSSTGCSDSQYFEELYNYYTT
Sbjct: 121 VNYSEGEILNRYRRAYEKPWGGSRLRYVSDGKPFWQYIVSSSTGCSDSQYFEELYNYYTT 180

Query: 181 KKAWHLCDPDAERVFKALRQAGVKIAVVSNFDTRLRPLLRALNCDHWFDAVAVSAEVEAE 240
            KAWHLCDPDAERVFKALRQAGVKIA+VSNFDTRLRPLLRAL CDHWFDAVAVSAEVEAE
Sbjct: 181 NKAWHLCDPDAERVFKALRQAGVKIAIVSNFDTRLRPLLRALKCDHWFDAVAVSAEVEAE 240

Query: 241 KPNPTIFLKACELLGVSPEDALHVGDDRRNDIWGARDAGCDAWLWGSDVHSFKEVAERIG 300
           KPNPTIFLKACELLGV PE+ALHVGDDRRND+WGARDAGCDAWLWGSDVHSFKEVAERIG
Sbjct: 241 KPNPTIFLKACELLGVRPEEALHVGDDRRNDVWGARDAGCDAWLWGSDVHSFKEVAERIG 300

Query: 301 VKV 304
           VKV
Sbjct: 301 VKV 303

BLAST of Bhi11G002301 vs. NCBI nr
Match: XP_022983839.1 (haloacid dehalogenase-like hydrolase domain-containing protein 3 [Cucurbita maxima])

HSP 1 Score: 570.9 bits (1470), Expect = 6.8e-159
Identity = 276/303 (91.09%), Postives = 287/303 (94.72%), Query Frame = 0

Query: 1   MALIATRTKISRILSSFSPKTLLSRPPFSSNGVQAAARLHPLGGFGPSFASSAVASMEPE 60
           MALI+ RTKISR+LS+ S K  LSR PF SNGVQ   RLHPLGGFG SFASSAVAS+E +
Sbjct: 1   MALISARTKISRVLSTLSHKNFLSRSPFFSNGVQPVDRLHPLGGFGRSFASSAVASVEHD 60

Query: 61  ALGNRAIGFEILGVKDYEDYRRSLYGEITHKALLVDAVGTLVVPSQPMAQIYREIGEKYG 120
           ALG+RA+GFEILGVKDYEDYRRSLYGEITHKALLVDAVGTLVVPSQPMAQIYREIGEKYG
Sbjct: 61  ALGSRAMGFEILGVKDYEDYRRSLYGEITHKALLVDAVGTLVVPSQPMAQIYREIGEKYG 120

Query: 121 VNYSEGEILNRYRRAYEKPWGRSRLRYVSDGKPFWQYIVSSSTGCSDSQYFEELYNYYTT 180
           VNYSEGEILNRYRRAYEKPWG SRLRYVSDGKPFWQYIVSSSTGCSDSQYFEELYNYYTT
Sbjct: 121 VNYSEGEILNRYRRAYEKPWGGSRLRYVSDGKPFWQYIVSSSTGCSDSQYFEELYNYYTT 180

Query: 181 KKAWHLCDPDAERVFKALRQAGVKIAVVSNFDTRLRPLLRALNCDHWFDAVAVSAEVEAE 240
            KAWHLCDPDAERVFKALRQAGVKIA+VSNFDTRLRPLLRAL CDHWFDAVAVSAEVEAE
Sbjct: 181 NKAWHLCDPDAERVFKALRQAGVKIAIVSNFDTRLRPLLRALKCDHWFDAVAVSAEVEAE 240

Query: 241 KPNPTIFLKACELLGVSPEDALHVGDDRRNDIWGARDAGCDAWLWGSDVHSFKEVAERIG 300
           KPNPTIFLKACELLGV PE+ALHVGDDRRND+WGARDAGCDAWLWGSDVHSF+EVAERIG
Sbjct: 241 KPNPTIFLKACELLGVRPEEALHVGDDRRNDVWGARDAGCDAWLWGSDVHSFREVAERIG 300

Query: 301 VKV 304
           VKV
Sbjct: 301 VKV 303

BLAST of Bhi11G002301 vs. ExPASy TrEMBL
Match: A0A0A0LCE6 (N-acylneuraminate-9-phosphatase OS=Cucumis sativus OX=3659 GN=Csa_3G821560 PE=4 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 5.8e-164
Identity = 283/303 (93.40%), Postives = 292/303 (96.37%), Query Frame = 0

Query: 1   MALIATRTKISRILSSFSPKTLLSRPPFSSNGVQAAARLHPLGGFGPSFASSAVASMEPE 60
           MALI+TRTKISRILS+FSP TLLSRPPF SNGVQAAARL P GGFG SFASSAVAS+EPE
Sbjct: 1   MALISTRTKISRILSTFSPNTLLSRPPFPSNGVQAAARLLPFGGFGSSFASSAVASVEPE 60

Query: 61  ALGNRAIGFEILGVKDYEDYRRSLYGEITHKALLVDAVGTLVVPSQPMAQIYREIGEKYG 120
            LG+  IGFEILGVKDYEDYRRSLYG+ITHKALLVDAVGTLVVPSQPMAQIYREIGEKYG
Sbjct: 61  TLGSSGIGFEILGVKDYEDYRRSLYGDITHKALLVDAVGTLVVPSQPMAQIYREIGEKYG 120

Query: 121 VNYSEGEILNRYRRAYEKPWGRSRLRYVSDGKPFWQYIVSSSTGCSDSQYFEELYNYYTT 180
           VNYSEGEILNRYRRAYEKPWGRSRLRYV+DGKPFWQYIVSSSTGCSDSQYFEELYNYYTT
Sbjct: 121 VNYSEGEILNRYRRAYEKPWGRSRLRYVNDGKPFWQYIVSSSTGCSDSQYFEELYNYYTT 180

Query: 181 KKAWHLCDPDAERVFKALRQAGVKIAVVSNFDTRLRPLLRALNCDHWFDAVAVSAEVEAE 240
            KAWHLCDPDAE+VFKALRQAGVKIA+VSNFDTRLRPLLR LNCDHWFDAVAVSAEVEAE
Sbjct: 181 NKAWHLCDPDAEKVFKALRQAGVKIAIVSNFDTRLRPLLRDLNCDHWFDAVAVSAEVEAE 240

Query: 241 KPNPTIFLKACELLGVSPEDALHVGDDRRNDIWGARDAGCDAWLWGSDVHSFKEVAERIG 300
           KPNPTIFLKACELLGV PEDALHVGDDRRND+WGARDAGCDAWLWGSDVHSFKEVAERIG
Sbjct: 241 KPNPTIFLKACELLGVRPEDALHVGDDRRNDVWGARDAGCDAWLWGSDVHSFKEVAERIG 300

Query: 301 VKV 304
           VKV
Sbjct: 301 VKV 303

BLAST of Bhi11G002301 vs. ExPASy TrEMBL
Match: A0A5D3DQC8 (Haloacid dehalogenase-like hydrolase domain-containing protein 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006810 PE=4 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 2.2e-163
Identity = 282/303 (93.07%), Postives = 290/303 (95.71%), Query Frame = 0

Query: 1   MALIATRTKISRILSSFSPKTLLSRPPFSSNGVQAAARLHPLGGFGPSFASSAVASMEPE 60
           MALIA RTKISR LS+FS  +LLSRPPF SNGVQA ARLHP GG G SF+SSAVASMEPE
Sbjct: 1   MALIAARTKISRTLSTFSLNSLLSRPPFPSNGVQAVARLHPFGGLGSSFSSSAVASMEPE 60

Query: 61  ALGNRAIGFEILGVKDYEDYRRSLYGEITHKALLVDAVGTLVVPSQPMAQIYREIGEKYG 120
            LG+R IGFEILGVKDYEDYRRSLYG+ITHKALLVDAVGTLVVPSQPMAQIYREIGEKYG
Sbjct: 61  TLGSRGIGFEILGVKDYEDYRRSLYGDITHKALLVDAVGTLVVPSQPMAQIYREIGEKYG 120

Query: 121 VNYSEGEILNRYRRAYEKPWGRSRLRYVSDGKPFWQYIVSSSTGCSDSQYFEELYNYYTT 180
           VNYSEGEILNRYRRAYEKPWGRSRLRYV+DGKPFWQYIVSSSTGCSDSQYFEELYNYYTT
Sbjct: 121 VNYSEGEILNRYRRAYEKPWGRSRLRYVNDGKPFWQYIVSSSTGCSDSQYFEELYNYYTT 180

Query: 181 KKAWHLCDPDAERVFKALRQAGVKIAVVSNFDTRLRPLLRALNCDHWFDAVAVSAEVEAE 240
            KAWHLCDPDAERVFKALRQAGVKIA+VSNFDTRLRPLLRALNCDHWFDAVAVSAEVEAE
Sbjct: 181 NKAWHLCDPDAERVFKALRQAGVKIAIVSNFDTRLRPLLRALNCDHWFDAVAVSAEVEAE 240

Query: 241 KPNPTIFLKACELLGVSPEDALHVGDDRRNDIWGARDAGCDAWLWGSDVHSFKEVAERIG 300
           KPNPTIFLKACELLGV PEDALHVGDDRRND+WGARDAGCDAWLWGSDVHSFKEVAERIG
Sbjct: 241 KPNPTIFLKACELLGVRPEDALHVGDDRRNDLWGARDAGCDAWLWGSDVHSFKEVAERIG 300

Query: 301 VKV 304
           VKV
Sbjct: 301 VKV 303

BLAST of Bhi11G002301 vs. ExPASy TrEMBL
Match: A0A1S3B7N8 (haloacid dehalogenase-like hydrolase domain-containing protein 3 OS=Cucumis melo OX=3656 GN=LOC103486892 PE=4 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 2.2e-163
Identity = 282/303 (93.07%), Postives = 290/303 (95.71%), Query Frame = 0

Query: 1   MALIATRTKISRILSSFSPKTLLSRPPFSSNGVQAAARLHPLGGFGPSFASSAVASMEPE 60
           MALIA RTKISR LS+FS  +LLSRPPF SNGVQA ARLHP GG G SF+SSAVASMEPE
Sbjct: 1   MALIAARTKISRTLSTFSLNSLLSRPPFPSNGVQAVARLHPFGGLGSSFSSSAVASMEPE 60

Query: 61  ALGNRAIGFEILGVKDYEDYRRSLYGEITHKALLVDAVGTLVVPSQPMAQIYREIGEKYG 120
            LG+R IGFEILGVKDYEDYRRSLYG+ITHKALLVDAVGTLVVPSQPMAQIYREIGEKYG
Sbjct: 61  TLGSRGIGFEILGVKDYEDYRRSLYGDITHKALLVDAVGTLVVPSQPMAQIYREIGEKYG 120

Query: 121 VNYSEGEILNRYRRAYEKPWGRSRLRYVSDGKPFWQYIVSSSTGCSDSQYFEELYNYYTT 180
           VNYSEGEILNRYRRAYEKPWGRSRLRYV+DGKPFWQYIVSSSTGCSDSQYFEELYNYYTT
Sbjct: 121 VNYSEGEILNRYRRAYEKPWGRSRLRYVNDGKPFWQYIVSSSTGCSDSQYFEELYNYYTT 180

Query: 181 KKAWHLCDPDAERVFKALRQAGVKIAVVSNFDTRLRPLLRALNCDHWFDAVAVSAEVEAE 240
            KAWHLCDPDAERVFKALRQAGVKIA+VSNFDTRLRPLLRALNCDHWFDAVAVSAEVEAE
Sbjct: 181 NKAWHLCDPDAERVFKALRQAGVKIAIVSNFDTRLRPLLRALNCDHWFDAVAVSAEVEAE 240

Query: 241 KPNPTIFLKACELLGVSPEDALHVGDDRRNDIWGARDAGCDAWLWGSDVHSFKEVAERIG 300
           KPNPTIFLKACELLGV PEDALHVGDDRRND+WGARDAGCDAWLWGSDVHSFKEVAERIG
Sbjct: 241 KPNPTIFLKACELLGVRPEDALHVGDDRRNDLWGARDAGCDAWLWGSDVHSFKEVAERIG 300

Query: 301 VKV 304
           VKV
Sbjct: 301 VKV 303

BLAST of Bhi11G002301 vs. ExPASy TrEMBL
Match: A0A6J1FAW1 (haloacid dehalogenase-like hydrolase domain-containing protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111442399 PE=4 SV=1)

HSP 1 Score: 572.0 bits (1473), Expect = 1.5e-159
Identity = 277/303 (91.42%), Postives = 287/303 (94.72%), Query Frame = 0

Query: 1   MALIATRTKISRILSSFSPKTLLSRPPFSSNGVQAAARLHPLGGFGPSFASSAVASMEPE 60
           MALI+ RTKISR+LS+ S K  LSR PF SNGVQ   RLHPLGGFG SFASSAVAS+E +
Sbjct: 1   MALISARTKISRVLSTLSHKNFLSRSPFFSNGVQPVDRLHPLGGFGRSFASSAVASVEHD 60

Query: 61  ALGNRAIGFEILGVKDYEDYRRSLYGEITHKALLVDAVGTLVVPSQPMAQIYREIGEKYG 120
           ALG+RA+GFEILGVKDYEDYRRSLYGEITHKALLVDAVGTLVVPSQPMAQIYREIGEKYG
Sbjct: 61  ALGSRAMGFEILGVKDYEDYRRSLYGEITHKALLVDAVGTLVVPSQPMAQIYREIGEKYG 120

Query: 121 VNYSEGEILNRYRRAYEKPWGRSRLRYVSDGKPFWQYIVSSSTGCSDSQYFEELYNYYTT 180
           VNYSEGEILNRYRRAYEKPWG SRLRYVSDGKPFWQYIVSSSTGCSDSQYFEELYNYYTT
Sbjct: 121 VNYSEGEILNRYRRAYEKPWGGSRLRYVSDGKPFWQYIVSSSTGCSDSQYFEELYNYYTT 180

Query: 181 KKAWHLCDPDAERVFKALRQAGVKIAVVSNFDTRLRPLLRALNCDHWFDAVAVSAEVEAE 240
            KAWHLCDPDAERVFKALRQAGVKIA+VSNFDTRLRPLLRAL CDHWFDAVAVSAEVEAE
Sbjct: 181 NKAWHLCDPDAERVFKALRQAGVKIAIVSNFDTRLRPLLRALKCDHWFDAVAVSAEVEAE 240

Query: 241 KPNPTIFLKACELLGVSPEDALHVGDDRRNDIWGARDAGCDAWLWGSDVHSFKEVAERIG 300
           KPNPTIFLKACELLGV PE+ALHVGDDRRND+WGARDAGCDAWLWGSDVHSFKEVAERIG
Sbjct: 241 KPNPTIFLKACELLGVRPEEALHVGDDRRNDVWGARDAGCDAWLWGSDVHSFKEVAERIG 300

Query: 301 VKV 304
           VKV
Sbjct: 301 VKV 303

BLAST of Bhi11G002301 vs. ExPASy TrEMBL
Match: A0A6J1J8M8 (haloacid dehalogenase-like hydrolase domain-containing protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111482334 PE=4 SV=1)

HSP 1 Score: 570.9 bits (1470), Expect = 3.3e-159
Identity = 276/303 (91.09%), Postives = 287/303 (94.72%), Query Frame = 0

Query: 1   MALIATRTKISRILSSFSPKTLLSRPPFSSNGVQAAARLHPLGGFGPSFASSAVASMEPE 60
           MALI+ RTKISR+LS+ S K  LSR PF SNGVQ   RLHPLGGFG SFASSAVAS+E +
Sbjct: 1   MALISARTKISRVLSTLSHKNFLSRSPFFSNGVQPVDRLHPLGGFGRSFASSAVASVEHD 60

Query: 61  ALGNRAIGFEILGVKDYEDYRRSLYGEITHKALLVDAVGTLVVPSQPMAQIYREIGEKYG 120
           ALG+RA+GFEILGVKDYEDYRRSLYGEITHKALLVDAVGTLVVPSQPMAQIYREIGEKYG
Sbjct: 61  ALGSRAMGFEILGVKDYEDYRRSLYGEITHKALLVDAVGTLVVPSQPMAQIYREIGEKYG 120

Query: 121 VNYSEGEILNRYRRAYEKPWGRSRLRYVSDGKPFWQYIVSSSTGCSDSQYFEELYNYYTT 180
           VNYSEGEILNRYRRAYEKPWG SRLRYVSDGKPFWQYIVSSSTGCSDSQYFEELYNYYTT
Sbjct: 121 VNYSEGEILNRYRRAYEKPWGGSRLRYVSDGKPFWQYIVSSSTGCSDSQYFEELYNYYTT 180

Query: 181 KKAWHLCDPDAERVFKALRQAGVKIAVVSNFDTRLRPLLRALNCDHWFDAVAVSAEVEAE 240
            KAWHLCDPDAERVFKALRQAGVKIA+VSNFDTRLRPLLRAL CDHWFDAVAVSAEVEAE
Sbjct: 181 NKAWHLCDPDAERVFKALRQAGVKIAIVSNFDTRLRPLLRALKCDHWFDAVAVSAEVEAE 240

Query: 241 KPNPTIFLKACELLGVSPEDALHVGDDRRNDIWGARDAGCDAWLWGSDVHSFKEVAERIG 300
           KPNPTIFLKACELLGV PE+ALHVGDDRRND+WGARDAGCDAWLWGSDVHSF+EVAERIG
Sbjct: 241 KPNPTIFLKACELLGVRPEEALHVGDDRRNDVWGARDAGCDAWLWGSDVHSFREVAERIG 300

Query: 301 VKV 304
           VKV
Sbjct: 301 VKV 303

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT2G41250.11.4e-11776.17Haloacid dehalogenase-like hydrolase (HAD) superfamily protein [more]
AT1G14310.15.3e-5348.10Haloacid dehalogenase-like hydrolase (HAD) superfamily protein [more]
Match NameE-valueIdentityDescription
Q5HZL91.7e-1630.65Haloacid dehalogenase-like hydrolase domain-containing protein 3 OS=Xenopus laev... [more]
Q9CYW41.5e-1529.47Haloacid dehalogenase-like hydrolase domain-containing protein 3 OS=Mus musculus... [more]
Q949153.3e-1528.23Rhythmically expressed gene 2 protein OS=Drosophila melanogaster OX=7227 GN=Reg-... [more]
O142623.3e-1528.43Putative uncharacterized hydrolase C7D4.05 OS=Schizosaccharomyces pombe (strain ... [more]
Q5E9D62.8e-1427.45Haloacid dehalogenase-like hydrolase domain-containing protein 3 OS=Bos taurus O... [more]
Match NameE-valueIdentityDescription
XP_038905682.15.7e-174100.00haloacid dehalogenase-like hydrolase domain-containing protein 3 [Benincasa hisp... [more]
XP_011652195.11.2e-16393.40haloacid dehalogenase-like hydrolase domain-containing protein 3 [Cucumis sativu... [more]
XP_008443269.14.5e-16393.07PREDICTED: haloacid dehalogenase-like hydrolase domain-containing protein 3 [Cuc... [more]
XP_022935563.13.0e-15991.42haloacid dehalogenase-like hydrolase domain-containing protein 3 [Cucurbita mosc... [more]
XP_022983839.16.8e-15991.09haloacid dehalogenase-like hydrolase domain-containing protein 3 [Cucurbita maxi... [more]
Match NameE-valueIdentityDescription
A0A0A0LCE65.8e-16493.40N-acylneuraminate-9-phosphatase OS=Cucumis sativus OX=3659 GN=Csa_3G821560 PE=4 ... [more]
A0A5D3DQC82.2e-16393.07Haloacid dehalogenase-like hydrolase domain-containing protein 3 OS=Cucumis melo... [more]
A0A1S3B7N82.2e-16393.07haloacid dehalogenase-like hydrolase domain-containing protein 3 OS=Cucumis melo... [more]
A0A6J1FAW11.5e-15991.42haloacid dehalogenase-like hydrolase domain-containing protein 3 OS=Cucurbita mo... [more]
A0A6J1J8M83.3e-15991.09haloacid dehalogenase-like hydrolase domain-containing protein 3 OS=Cucurbita ma... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006439HAD hydrolase, subfamily IAPRINTSPR00413HADHALOGNASEcoord: 90..101
score: 37.5
coord: 246..266
score: 25.0
coord: 198..211
score: 38.1
coord: 228..244
score: 34.31
IPR006439HAD hydrolase, subfamily IATIGRFAMTIGR01549TIGR01549coord: 199..279
e-value: 8.4E-13
score: 46.9
IPR006439HAD hydrolase, subfamily IATIGRFAMTIGR01509TIGR01509coord: 181..281
e-value: 8.7E-8
score: 30.5
IPR011949HAD-superfamily hydrolase, subfamily IA, REG-2-likeTIGRFAMTIGR02252TIGR02252coord: 91..284
e-value: 3.8E-63
score: 210.9
IPR041492Haloacid dehalogenase-like hydrolasePFAMPF13419HAD_2coord: 99..282
e-value: 3.6E-18
score: 66.3
IPR044924HAD-superfamily hydrolase, subfamily IA, REG-2-like, capping domain superfamilyGENE3D1.10.150.720coord: 106..179
e-value: 1.4E-45
score: 157.6
IPR023214HAD superfamilyGENE3D3.40.50.1000coord: 95..287
e-value: 1.4E-45
score: 157.6
NoneNo IPR availableSFLDSFLDS00003Haloacid_Dehalogenasecoord: 91..285
e-value: 7.6E-26
score: 85.8
NoneNo IPR availableSFLDSFLDG01129C1.5:_HAD,_Beta-PGM,_Phosphatcoord: 91..285
e-value: 7.6E-26
score: 85.8
NoneNo IPR availablePANTHERPTHR46649:SF6HALOACID DEHALOGENASE-LIKE HYDROLASE (HAD) SUPERFAMILY PROTEINcoord: 51..303
NoneNo IPR availablePANTHERPTHR46649FAMILY NOT NAMEDcoord: 51..303
NoneNo IPR availableCDDcd16415HAD_dREG-2_likecoord: 191..302
e-value: 1.0417E-49
score: 159.379
IPR036412HAD-like superfamilySUPERFAMILY56784HAD-likecoord: 91..293

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi11M002301Bhi11M002301mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity