Bhi11G001865 (gene) Wax gourd (B227) v1

Overview
NameBhi11G001865
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationchr11: 61366176 .. 61367534 (+)
RNA-Seq ExpressionBhi11G001865
SyntenyBhi11G001865
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTCTTCTTCTTTATCTTTTTCAAGAAAATAAGTTGTGTGTGATTGGATCTCGTCTAGTGGTAGTAGTCATGTATTTATTTCATAACCAATTCTTCTACGACATTGTAATCAAAGATGTAAACTAAAATTGACATGCTAAACATAAGAATTGAGATAGACAATTTTTTTATTTTTTGAAAAACAAATGTAAGATCCAAAGATTTAAACTTCTCTACACGAAGTACGTTCAAGATAAATTAGAGTTTTTTTTTCTTAAAAAAAATAAAAACAATTACAAATTTTCTTTATTCAATTTAATTTAGGTTTGTTTAATTTTTTTTTTTTAAAGAAAACAATAATCCTACAAATCCAAACAAAAATAATAAAACTCGAATCTTGCATGAACATGAGCTCCCAAATGATAAGAAACCAAATCCACAGCCTTGCTAGCCCCTACACTTCCCCGCCATGGACAAAAGCGCCTCCTTGATGAATCGATCGCCGCCGCCATCCCCGACGCCGCATCGGACCGTTGATTTCTCTCCGATCGAGTTTATTCTCGGCCTCGTTGCCGTCATCACAATCCCTGCCCTGGTCTACTCCTTCTTCTTCGCCATCAAGTGCCCGCCCAATCTCCTCCGCCTCTGGCGGCATAGATCCGCCAGGCTCTCCGGCGCCGCCGTCACCGTGGTGGTCAGAAATCGTACTCCTTCTAGTGACGTGGCTGCAGCCGTTAAATACAACAAGGACGAGCACTGTAAGGAAGTCAGCAACGAGTGTCCGGTGTGCCTATCGGCGTTCGACGACGGCGAGGAGATCCGGCAACTCGCCGCCTGTAAACACTCTTTCCATGTGGATTGTATCGACATGTGGCTCTATTCTCACCCTAATTGCCCTGTTTGCCGCGCCGCCGTCCCTGTCGCTGTTAAACGGCCGATTAACAACCACGCGCCGCGGCGGCCGGCGGTTTCTAGAAGCGACGATTTCCAGCAAGGACTGCCGGATGCGGGATATTTAGTGTGAAATTCTTCATCTTCTTCCATCTCTGTTCCCTTCCGTTTGCTGCAAATTCGAACCACAGATCGAAAATTACGTTCAAATATCGTCTCAATTTCGTTAAATTTTGTGGATAATTTGGAATTAGATAGGTTTTGATTTCAAAGAGTTTTGAAATTTGGATTGTTTTTTTATTCTGAAAAATTCAAAGCGCAAGATTGAAACAGAATTAGGTTTTTTTTTTAATGGGAAATTGATTTATGAAATATATTTCCCTTTTCTTCTTCCTATTACACGTGTTATTATTTGATTCCATTCCCCACTCTAGATTAAATGCGAATATATAATTTCAGTCTTATAGTATATCTATATTTTGCTTACG

mRNA sequence

TTTTCTTCTTCTTTATCTTTTTCAAGAAAATAAGTTGTGTGTGATTGGATCTCGTCTAGTGGTAGTAGTCATGTATTTATTTCATAACCAATTCTTCTACGACATTGTAATCAAAGATGTAAACTAAAATTGACATGCTAAACATAAGAATTGAGATAGACAATTTTTTTATTTTTTGAAAAACAAATGTAAGATCCAAAGATTTAAACTTCTCTACACGAAGTACGTTCAAGATAAATTAGAGTTTTTTTTTCTTAAAAAAAATAAAAACAATTACAAATTTTCTTTATTCAATTTAATTTAGGTTTGTTTAATTTTTTTTTTTTAAAGAAAACAATAATCCTACAAATCCAAACAAAAATAATAAAACTCGAATCTTGCATGAACATGAGCTCCCAAATGATAAGAAACCAAATCCACAGCCTTGCTAGCCCCTACACTTCCCCGCCATGGACAAAAGCGCCTCCTTGATGAATCGATCGCCGCCGCCATCCCCGACGCCGCATCGGACCGTTGATTTCTCTCCGATCGAGTTTATTCTCGGCCTCGTTGCCGTCATCACAATCCCTGCCCTGGTCTACTCCTTCTTCTTCGCCATCAAGTGCCCGCCCAATCTCCTCCGCCTCTGGCGGCATAGATCCGCCAGGCTCTCCGGCGCCGCCGTCACCGTGGTGGTCAGAAATCGTACTCCTTCTAGTGACGTGGCTGCAGCCGTTAAATACAACAAGGACGAGCACTGTAAGGAAGTCAGCAACGAGTGTCCGGTGTGCCTATCGGCGTTCGACGACGGCGAGGAGATCCGGCAACTCGCCGCCTGTAAACACTCTTTCCATGTGGATTGTATCGACATGTGGCTCTATTCTCACCCTAATTGCCCTGTTTGCCGCGCCGCCGTCCCTGTCGCTGTTAAACGGCCGATTAACAACCACGCGCCGCGGCGGCCGGCGGTTTCTAGAAGCGACGATTTCCAGCAAGGACTGCCGGATGCGGGATATTTAGTGTGAAATTCTTCATCTTCTTCCATCTCTGTTCCCTTCCGTTTGCTGCAAATTCGAACCACAGATCGAAAATTACGTTCAAATATCGTCTCAATTTCGTTAAATTTTGTGGATAATTTGGAATTAGATAGGTTTTGATTTCAAAGAGTTTTGAAATTTGGATTGTTTTTTTATTCTGAAAAATTCAAAGCGCAAGATTGAAACAGAATTAGGTTTTTTTTTTAATGGGAAATTGATTTATGAAATATATTTCCCTTTTCTTCTTCCTATTACACGTGTTATTATTTGATTCCATTCCCCACTCTAGATTAAATGCGAATATATAATTTCAGTCTTATAGTATATCTATATTTTGCTTACG

Coding sequence (CDS)

ATGGACAAAAGCGCCTCCTTGATGAATCGATCGCCGCCGCCATCCCCGACGCCGCATCGGACCGTTGATTTCTCTCCGATCGAGTTTATTCTCGGCCTCGTTGCCGTCATCACAATCCCTGCCCTGGTCTACTCCTTCTTCTTCGCCATCAAGTGCCCGCCCAATCTCCTCCGCCTCTGGCGGCATAGATCCGCCAGGCTCTCCGGCGCCGCCGTCACCGTGGTGGTCAGAAATCGTACTCCTTCTAGTGACGTGGCTGCAGCCGTTAAATACAACAAGGACGAGCACTGTAAGGAAGTCAGCAACGAGTGTCCGGTGTGCCTATCGGCGTTCGACGACGGCGAGGAGATCCGGCAACTCGCCGCCTGTAAACACTCTTTCCATGTGGATTGTATCGACATGTGGCTCTATTCTCACCCTAATTGCCCTGTTTGCCGCGCCGCCGTCCCTGTCGCTGTTAAACGGCCGATTAACAACCACGCGCCGCGGCGGCCGGCGGTTTCTAGAAGCGACGATTTCCAGCAAGGACTGCCGGATGCGGGATATTTAGTGTGA

Protein sequence

MDKSASLMNRSPPPSPTPHRTVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLLRLWRHRSARLSGAAVTVVVRNRTPSSDVAAAVKYNKDEHCKEVSNECPVCLSAFDDGEEIRQLAACKHSFHVDCIDMWLYSHPNCPVCRAAVPVAVKRPINNHAPRRPAVSRSDDFQQGLPDAGYLV
Homology
BLAST of Bhi11G001865 vs. TAIR 10
Match: AT2G37580.1 (RING/U-box superfamily protein )

HSP 1 Score: 141.4 bits (355), Expect = 7.5e-34
Identity = 77/191 (40.31%), Postives = 106/191 (55.50%), Query Frame = 0

Query: 12  PPPSPTPHRTVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLLRLWRHRSARLS--- 71
           PPP P P R++D +P++ I  ++A + +PALVY+ FF   C  +       R++  S   
Sbjct: 47  PPPFPAPPRSIDLTPLKLIFVVIAFVAVPALVYALFFNGPCSSSRRNSSSSRTSSSSDDT 106

Query: 72  --GAAVTVVVRNRTPSSDVAAAVKYNKDEHCKEVSNECPVCLSAFDDGEEIRQLAACKHS 131
                 T  +   T +S+  +  K++KD H KE+ NEC VCL  F D +E+RQL+ CKH+
Sbjct: 107 PHATVDTPPITETTVTSE--SGGKFHKDTHSKEIGNECSVCLMVFTDSDELRQLSECKHA 166

Query: 132 FHVDCIDMWLYSHPNCPVCRA-----------AVPVAVKRPINNHAPRR--PAVSRSDDF 185
           FHV CI+ WL  HPNCP+CR             VPV V   +N     R   A SR DD+
Sbjct: 167 FHVLCIETWLKDHPNCPICRTDVSVKQQTEAPNVPVNVNGNVNRSGGNRRVSATSRDDDW 226

BLAST of Bhi11G001865 vs. TAIR 10
Match: AT5G43420.1 (RING/U-box superfamily protein )

HSP 1 Score: 73.9 bits (180), Expect = 1.5e-13
Identity = 57/167 (34.13%), Postives = 79/167 (47.31%), Query Frame = 0

Query: 12  PPPSPTPHRTVDFSPIEFILGLVAVITIPA----LVYSFFFAIKCPPNLLRL-------- 71
           PPP P  HR    +   F +  VAVI I A    LV  + F IKC  N  R+        
Sbjct: 18  PPPPPIFHR-ASSTGTSFPILAVAVIGILATAFLLVSYYVFVIKCCLNWHRIDILGRFSL 77

Query: 72  -WRHRSARLSGAAVTVVVRNRTPSSDVAAAV---KYNK-------------DEHCKEVSN 131
             R R+ +      +  +R+R     V  A+   K+ K             +E  ++ S 
Sbjct: 78  SRRRRNDQDPLMVYSPELRSRGLDESVIRAIPIFKFKKRYDQNDGVFTGEGEEEEEKRSQ 137

Query: 132 ECPVCLSAFDDGEEIRQLAACKHSFHVDCIDMWLYSHPNCPVCRAAV 150
           EC VCLS F D E++R +  C H FH+DCID+WL ++ NCP+CR  V
Sbjct: 138 ECSVCLSEFQDEEKLRIIPNCSHLFHIDCIDVWLQNNANCPLCRTRV 183

BLAST of Bhi11G001865 vs. TAIR 10
Match: AT1G72220.1 (RING/U-box superfamily protein )

HSP 1 Score: 73.6 bits (179), Expect = 1.9e-13
Identity = 26/47 (55.32%), Postives = 35/47 (74.47%), Query Frame = 0

Query: 103 ECPVCLSAFDDGEEIRQLAACKHSFHVDCIDMWLYSHPNCPVCRAAV 150
           +CPVCL+ F++ E +R L  C H+FH+ CID WL SH NCP+CRA +
Sbjct: 176 DCPVCLNEFEEDESLRLLPKCNHAFHISCIDTWLSSHTNCPLCRAGI 222

BLAST of Bhi11G001865 vs. TAIR 10
Match: AT4G40070.1 (RING/U-box superfamily protein )

HSP 1 Score: 73.6 bits (179), Expect = 1.9e-13
Identity = 48/156 (30.77%), Postives = 73/156 (46.79%), Query Frame = 0

Query: 4   SASLMNRSPP---PSPTPHRTVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLLRLW 63
           +A   + SPP      TP +T  F+ +  +  L  +++    VY    A   P +  R +
Sbjct: 27  NAQSFSPSPPDLQTGHTPSKTTVFAVLVTLFFLTGLLS----VYIRHCARSNPDSSTRYF 86

Query: 64  RHRSARLSGAAVTVVVRNRTPSSDVAAAVKYNKDEHCKEVSNECPVCLSAFDDGEEIRQL 123
           R+R+    G++    + N    S    A    K+        EC +CL+  +D E +R L
Sbjct: 87  RNRAN--DGSSRRGGLDNAVVESFPVFAYSSVKESKIGSKDLECAICLNELEDHETVRLL 146

Query: 124 AACKHSFHVDCIDMWLYSHPNCPVCRAAVPVAVKRP 157
             C H FH+DCID WLYSH  CPVCR+ +     +P
Sbjct: 147 PICNHLFHIDCIDTWLYSHATCPVCRSNLTAKSNKP 176

BLAST of Bhi11G001865 vs. TAIR 10
Match: AT4G15975.1 (RING/U-box superfamily protein )

HSP 1 Score: 73.6 bits (179), Expect = 1.9e-13
Identity = 28/47 (59.57%), Postives = 35/47 (74.47%), Query Frame = 0

Query: 103 ECPVCLSAFDDGEEIRQLAACKHSFHVDCIDMWLYSHPNCPVCRAAV 150
           EC VCLS F D E  R +  CKH+FHVDCIDMW +SH +CP+CR+ +
Sbjct: 75  ECSVCLSEFKDNESGRVMPNCKHTFHVDCIDMWFHSHSSCPLCRSLI 121

BLAST of Bhi11G001865 vs. ExPASy Swiss-Prot
Match: O80927 (RING-H2 finger protein ATL33 OS=Arabidopsis thaliana OX=3702 GN=ATL33 PE=2 SV=2)

HSP 1 Score: 141.4 bits (355), Expect = 1.1e-32
Identity = 77/191 (40.31%), Postives = 106/191 (55.50%), Query Frame = 0

Query: 12  PPPSPTPHRTVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLLRLWRHRSARLS--- 71
           PPP P P R++D +P++ I  ++A + +PALVY+ FF   C  +       R++  S   
Sbjct: 47  PPPFPAPPRSIDLTPLKLIFVVIAFVAVPALVYALFFNGPCSSSRRNSSSSRTSSSSDDT 106

Query: 72  --GAAVTVVVRNRTPSSDVAAAVKYNKDEHCKEVSNECPVCLSAFDDGEEIRQLAACKHS 131
                 T  +   T +S+  +  K++KD H KE+ NEC VCL  F D +E+RQL+ CKH+
Sbjct: 107 PHATVDTPPITETTVTSE--SGGKFHKDTHSKEIGNECSVCLMVFTDSDELRQLSECKHA 166

Query: 132 FHVDCIDMWLYSHPNCPVCRA-----------AVPVAVKRPINNHAPRR--PAVSRSDDF 185
           FHV CI+ WL  HPNCP+CR             VPV V   +N     R   A SR DD+
Sbjct: 167 FHVLCIETWLKDHPNCPICRTDVSVKQQTEAPNVPVNVNGNVNRSGGNRRVSATSRDDDW 226

BLAST of Bhi11G001865 vs. ExPASy Swiss-Prot
Match: Q9LRB7 (E3 ubiquitin-protein ligase EL5 OS=Oryza sativa subsp. japonica OX=39947 GN=EL5.1 PE=1 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 2.4e-13
Identity = 43/101 (42.57%), Postives = 51/101 (50.50%), Query Frame = 0

Query: 83  SDVAAAVKYNKDEHCKEVSNECPVCLSAFDDGEEIRQLAACKHSFHVDCIDMWLYSHPNC 142
           S  AAA K  ++E    V  EC VCL+  +DGEE R L  C H FH +C+DMWL SH  C
Sbjct: 115 STAAAAAKEEEEEDDDGV--ECAVCLAELEDGEEARFLPRCGHGFHAECVDMWLGSHSTC 174

Query: 143 PVCR--AAVPVAVKRPINNHAPRRPAVSRSDDFQQGLPDAG 182
           P+CR    VP     P+    P    VS       GL D G
Sbjct: 175 PLCRLTVVVPPPPLPPVPPEPPASYTVSLPASVLLGLSDHG 213

BLAST of Bhi11G001865 vs. ExPASy Swiss-Prot
Match: Q9LSW9 (RING-H2 finger protein ATL16 OS=Arabidopsis thaliana OX=3702 GN=ATL16 PE=2 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 2.1e-12
Identity = 57/167 (34.13%), Postives = 79/167 (47.31%), Query Frame = 0

Query: 12  PPPSPTPHRTVDFSPIEFILGLVAVITIPA----LVYSFFFAIKCPPNLLRL-------- 71
           PPP P  HR    +   F +  VAVI I A    LV  + F IKC  N  R+        
Sbjct: 18  PPPPPIFHR-ASSTGTSFPILAVAVIGILATAFLLVSYYVFVIKCCLNWHRIDILGRFSL 77

Query: 72  -WRHRSARLSGAAVTVVVRNRTPSSDVAAAV---KYNK-------------DEHCKEVSN 131
             R R+ +      +  +R+R     V  A+   K+ K             +E  ++ S 
Sbjct: 78  SRRRRNDQDPLMVYSPELRSRGLDESVIRAIPIFKFKKRYDQNDGVFTGEGEEEEEKRSQ 137

Query: 132 ECPVCLSAFDDGEEIRQLAACKHSFHVDCIDMWLYSHPNCPVCRAAV 150
           EC VCLS F D E++R +  C H FH+DCID+WL ++ NCP+CR  V
Sbjct: 138 ECSVCLSEFQDEEKLRIIPNCSHLFHIDCIDVWLQNNANCPLCRTRV 183

BLAST of Bhi11G001865 vs. ExPASy Swiss-Prot
Match: Q8W571 (RING-H2 finger protein ATL32 OS=Arabidopsis thaliana OX=3702 GN=ATL32 PE=2 SV=3)

HSP 1 Score: 73.6 bits (179), Expect = 2.7e-12
Identity = 48/156 (30.77%), Postives = 73/156 (46.79%), Query Frame = 0

Query: 4   SASLMNRSPP---PSPTPHRTVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLLRLW 63
           +A   + SPP      TP +T  F+ +  +  L  +++    VY    A   P +  R +
Sbjct: 27  NAQSFSPSPPDLQTGHTPSKTTVFAVLVTLFFLTGLLS----VYIRHCARSNPDSSTRYF 86

Query: 64  RHRSARLSGAAVTVVVRNRTPSSDVAAAVKYNKDEHCKEVSNECPVCLSAFDDGEEIRQL 123
           R+R+    G++    + N    S    A    K+        EC +CL+  +D E +R L
Sbjct: 87  RNRAN--DGSSRRGGLDNAVVESFPVFAYSSVKESKIGSKDLECAICLNELEDHETVRLL 146

Query: 124 AACKHSFHVDCIDMWLYSHPNCPVCRAAVPVAVKRP 157
             C H FH+DCID WLYSH  CPVCR+ +     +P
Sbjct: 147 PICNHLFHIDCIDTWLYSHATCPVCRSNLTAKSNKP 176

BLAST of Bhi11G001865 vs. ExPASy Swiss-Prot
Match: Q8LFY8 (RING-H2 finger protein ATL54 OS=Arabidopsis thaliana OX=3702 GN=ATL54 PE=2 SV=2)

HSP 1 Score: 73.6 bits (179), Expect = 2.7e-12
Identity = 26/47 (55.32%), Postives = 35/47 (74.47%), Query Frame = 0

Query: 103 ECPVCLSAFDDGEEIRQLAACKHSFHVDCIDMWLYSHPNCPVCRAAV 150
           +CPVCL+ F++ E +R L  C H+FH+ CID WL SH NCP+CRA +
Sbjct: 176 DCPVCLNEFEEDESLRLLPKCNHAFHISCIDTWLSSHTNCPLCRAGI 222

BLAST of Bhi11G001865 vs. NCBI nr
Match: XP_038905078.1 (RING-H2 finger protein ATL33 [Benincasa hispida])

HSP 1 Score: 381.7 bits (979), Expect = 3.5e-102
Identity = 184/184 (100.00%), Postives = 184/184 (100.00%), Query Frame = 0

Query: 1   MDKSASLMNRSPPPSPTPHRTVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLLRLW 60
           MDKSASLMNRSPPPSPTPHRTVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLLRLW
Sbjct: 1   MDKSASLMNRSPPPSPTPHRTVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLLRLW 60

Query: 61  RHRSARLSGAAVTVVVRNRTPSSDVAAAVKYNKDEHCKEVSNECPVCLSAFDDGEEIRQL 120
           RHRSARLSGAAVTVVVRNRTPSSDVAAAVKYNKDEHCKEVSNECPVCLSAFDDGEEIRQL
Sbjct: 61  RHRSARLSGAAVTVVVRNRTPSSDVAAAVKYNKDEHCKEVSNECPVCLSAFDDGEEIRQL 120

Query: 121 AACKHSFHVDCIDMWLYSHPNCPVCRAAVPVAVKRPINNHAPRRPAVSRSDDFQQGLPDA 180
           AACKHSFHVDCIDMWLYSHPNCPVCRAAVPVAVKRPINNHAPRRPAVSRSDDFQQGLPDA
Sbjct: 121 AACKHSFHVDCIDMWLYSHPNCPVCRAAVPVAVKRPINNHAPRRPAVSRSDDFQQGLPDA 180

Query: 181 GYLV 185
           GYLV
Sbjct: 181 GYLV 184

BLAST of Bhi11G001865 vs. NCBI nr
Match: KAE8651013.1 (hypothetical protein Csa_001104 [Cucumis sativus])

HSP 1 Score: 349.4 bits (895), Expect = 1.9e-92
Identity = 167/184 (90.76%), Postives = 169/184 (91.85%), Query Frame = 0

Query: 1   MDKSASLMNRSPPPSPTPHRTVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLLRLW 60
           MD +AS MNRSPPP PTPHRTVDFSPIEFILGLVAVITIPALVYSF FAIKCPPN LRLW
Sbjct: 42  MDATASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALVYSFIFAIKCPPNFLRLW 101

Query: 61  RHRSARLSGAAVTVVVRNRTPSSDVAAAVKYNKDEHCKEVSNECPVCLSAFDDGEEIRQL 120
           R RS RLSGA  TVVV NRTPSSDVAAAVKYNKDEHCKEV NECPVCLSAFDDGEEIRQL
Sbjct: 102 RQRSTRLSGAGATVVVGNRTPSSDVAAAVKYNKDEHCKEVGNECPVCLSAFDDGEEIRQL 161

Query: 121 AACKHSFHVDCIDMWLYSHPNCPVCRAAVPVAVKRPINNHAPRRPAVSRSDDFQQGLPDA 180
           A CKHSFHVDCIDMWLYSHPNCPVCRAAVPV VKRPI+NHAPRRPA SRSDDF QGLPDA
Sbjct: 162 AVCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPIHNHAPRRPAASRSDDFHQGLPDA 221

Query: 181 GYLV 185
           GYLV
Sbjct: 222 GYLV 225

BLAST of Bhi11G001865 vs. NCBI nr
Match: XP_004137943.1 (RING-H2 finger protein ATL33 [Cucumis sativus])

HSP 1 Score: 349.4 bits (895), Expect = 1.9e-92
Identity = 167/184 (90.76%), Postives = 169/184 (91.85%), Query Frame = 0

Query: 1   MDKSASLMNRSPPPSPTPHRTVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLLRLW 60
           MD +AS MNRSPPP PTPHRTVDFSPIEFILGLVAVITIPALVYSF FAIKCPPN LRLW
Sbjct: 1   MDATASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALVYSFIFAIKCPPNFLRLW 60

Query: 61  RHRSARLSGAAVTVVVRNRTPSSDVAAAVKYNKDEHCKEVSNECPVCLSAFDDGEEIRQL 120
           R RS RLSGA  TVVV NRTPSSDVAAAVKYNKDEHCKEV NECPVCLSAFDDGEEIRQL
Sbjct: 61  RQRSTRLSGAGATVVVGNRTPSSDVAAAVKYNKDEHCKEVGNECPVCLSAFDDGEEIRQL 120

Query: 121 AACKHSFHVDCIDMWLYSHPNCPVCRAAVPVAVKRPINNHAPRRPAVSRSDDFQQGLPDA 180
           A CKHSFHVDCIDMWLYSHPNCPVCRAAVPV VKRPI+NHAPRRPA SRSDDF QGLPDA
Sbjct: 121 AVCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPIHNHAPRRPAASRSDDFHQGLPDA 180

Query: 181 GYLV 185
           GYLV
Sbjct: 181 GYLV 184

BLAST of Bhi11G001865 vs. NCBI nr
Match: XP_008442561.1 (PREDICTED: RING-H2 finger protein ATL33 [Cucumis melo] >KAA0044082.1 RING-H2 finger protein ATL33 [Cucumis melo var. makuwa] >TYK25055.1 RING-H2 finger protein ATL33 [Cucumis melo var. makuwa])

HSP 1 Score: 344.0 bits (881), Expect = 8.2e-91
Identity = 164/184 (89.13%), Postives = 166/184 (90.22%), Query Frame = 0

Query: 1   MDKSASLMNRSPPPSPTPHRTVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLLRLW 60
           MD +AS MNRSPPP PTPHRTVDFSPIEFILGLVAVITIPAL YSF FAIKCPPN LRLW
Sbjct: 1   MDTTASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALAYSFIFAIKCPPNFLRLW 60

Query: 61  RHRSARLSGAAVTVVVRNRTPSSDVAAAVKYNKDEHCKEVSNECPVCLSAFDDGEEIRQL 120
           R RS RLS A VT+VV NRTPSSDVAAAVKYNKDEHCKEV  ECPVCLSAFDDGEEIRQL
Sbjct: 61  RQRSTRLSAAGVTMVVGNRTPSSDVAAAVKYNKDEHCKEVGTECPVCLSAFDDGEEIRQL 120

Query: 121 AACKHSFHVDCIDMWLYSHPNCPVCRAAVPVAVKRPINNHAPRRPAVSRSDDFQQGLPDA 180
             CKHSFHVDCIDMWLYSHPNCPVCRAAVPV VKRPINNHAPRRPA SRSDDF QGLPDA
Sbjct: 121 TTCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPINNHAPRRPAASRSDDFHQGLPDA 180

Query: 181 GYLV 185
           GYLV
Sbjct: 181 GYLV 184

BLAST of Bhi11G001865 vs. NCBI nr
Match: XP_022934546.1 (RING-H2 finger protein ATL33-like [Cucurbita moschata] >KAG6580552.1 RING-H2 finger protein ATL33, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017306.1 RING-H2 finger protein ATL33, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 311.6 bits (797), Expect = 4.5e-81
Identity = 152/184 (82.61%), Postives = 157/184 (85.33%), Query Frame = 0

Query: 1   MDKSASLMNRSPPPSPTPHRTVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLLRLW 60
           MD SA+LMNRS PP PTPHRT DFSPIEFILGL+AVI IPALVYSFFFAIKCPP LLRLW
Sbjct: 4   MDTSAALMNRSTPPHPTPHRTNDFSPIEFILGLIAVIAIPALVYSFFFAIKCPPGLLRLW 63

Query: 61  RHRSARLSGAAVTVVVRNRTPSSDVAAAVKYNKDEHCKEVSNECPVCLSAFDDGEEIRQL 120
           R RSARLSGA VTVV  NR+  SDVA   KYNKDE  KEV NECPVCLSAF DGEEIRQL
Sbjct: 64  RRRSARLSGAPVTVVAGNRSSYSDVAVTFKYNKDEVGKEVGNECPVCLSAFVDGEEIRQL 123

Query: 121 AACKHSFHVDCIDMWLYSHPNCPVCRAAVPVAVKRPINNHAPRRPAVSRSDDFQQGLPDA 180
            ACKHSFHVDCIDMW YSHPNCPVCRA + +AVKRPINN APRRPA SRSDDF QGLPDA
Sbjct: 124 IACKHSFHVDCIDMWFYSHPNCPVCRAEIAIAVKRPINNRAPRRPAASRSDDFHQGLPDA 183

Query: 181 GYLV 185
           G LV
Sbjct: 184 GNLV 187

BLAST of Bhi11G001865 vs. ExPASy TrEMBL
Match: A0A0A0LA67 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G734330 PE=3 SV=1)

HSP 1 Score: 349.4 bits (895), Expect = 9.4e-93
Identity = 167/184 (90.76%), Postives = 169/184 (91.85%), Query Frame = 0

Query: 1   MDKSASLMNRSPPPSPTPHRTVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLLRLW 60
           MD +AS MNRSPPP PTPHRTVDFSPIEFILGLVAVITIPALVYSF FAIKCPPN LRLW
Sbjct: 1   MDATASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALVYSFIFAIKCPPNFLRLW 60

Query: 61  RHRSARLSGAAVTVVVRNRTPSSDVAAAVKYNKDEHCKEVSNECPVCLSAFDDGEEIRQL 120
           R RS RLSGA  TVVV NRTPSSDVAAAVKYNKDEHCKEV NECPVCLSAFDDGEEIRQL
Sbjct: 61  RQRSTRLSGAGATVVVGNRTPSSDVAAAVKYNKDEHCKEVGNECPVCLSAFDDGEEIRQL 120

Query: 121 AACKHSFHVDCIDMWLYSHPNCPVCRAAVPVAVKRPINNHAPRRPAVSRSDDFQQGLPDA 180
           A CKHSFHVDCIDMWLYSHPNCPVCRAAVPV VKRPI+NHAPRRPA SRSDDF QGLPDA
Sbjct: 121 AVCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPIHNHAPRRPAASRSDDFHQGLPDA 180

Query: 181 GYLV 185
           GYLV
Sbjct: 181 GYLV 184

BLAST of Bhi11G001865 vs. ExPASy TrEMBL
Match: A0A5D3DN65 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001760 PE=3 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 3.9e-91
Identity = 164/184 (89.13%), Postives = 166/184 (90.22%), Query Frame = 0

Query: 1   MDKSASLMNRSPPPSPTPHRTVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLLRLW 60
           MD +AS MNRSPPP PTPHRTVDFSPIEFILGLVAVITIPAL YSF FAIKCPPN LRLW
Sbjct: 1   MDTTASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALAYSFIFAIKCPPNFLRLW 60

Query: 61  RHRSARLSGAAVTVVVRNRTPSSDVAAAVKYNKDEHCKEVSNECPVCLSAFDDGEEIRQL 120
           R RS RLS A VT+VV NRTPSSDVAAAVKYNKDEHCKEV  ECPVCLSAFDDGEEIRQL
Sbjct: 61  RQRSTRLSAAGVTMVVGNRTPSSDVAAAVKYNKDEHCKEVGTECPVCLSAFDDGEEIRQL 120

Query: 121 AACKHSFHVDCIDMWLYSHPNCPVCRAAVPVAVKRPINNHAPRRPAVSRSDDFQQGLPDA 180
             CKHSFHVDCIDMWLYSHPNCPVCRAAVPV VKRPINNHAPRRPA SRSDDF QGLPDA
Sbjct: 121 TTCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPINNHAPRRPAASRSDDFHQGLPDA 180

Query: 181 GYLV 185
           GYLV
Sbjct: 181 GYLV 184

BLAST of Bhi11G001865 vs. ExPASy TrEMBL
Match: A0A1S3B6Q9 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103486397 PE=3 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 3.9e-91
Identity = 164/184 (89.13%), Postives = 166/184 (90.22%), Query Frame = 0

Query: 1   MDKSASLMNRSPPPSPTPHRTVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLLRLW 60
           MD +AS MNRSPPP PTPHRTVDFSPIEFILGLVAVITIPAL YSF FAIKCPPN LRLW
Sbjct: 1   MDTTASSMNRSPPPYPTPHRTVDFSPIEFILGLVAVITIPALAYSFIFAIKCPPNFLRLW 60

Query: 61  RHRSARLSGAAVTVVVRNRTPSSDVAAAVKYNKDEHCKEVSNECPVCLSAFDDGEEIRQL 120
           R RS RLS A VT+VV NRTPSSDVAAAVKYNKDEHCKEV  ECPVCLSAFDDGEEIRQL
Sbjct: 61  RQRSTRLSAAGVTMVVGNRTPSSDVAAAVKYNKDEHCKEVGTECPVCLSAFDDGEEIRQL 120

Query: 121 AACKHSFHVDCIDMWLYSHPNCPVCRAAVPVAVKRPINNHAPRRPAVSRSDDFQQGLPDA 180
             CKHSFHVDCIDMWLYSHPNCPVCRAAVPV VKRPINNHAPRRPA SRSDDF QGLPDA
Sbjct: 121 TTCKHSFHVDCIDMWLYSHPNCPVCRAAVPVTVKRPINNHAPRRPAASRSDDFHQGLPDA 180

Query: 181 GYLV 185
           GYLV
Sbjct: 181 GYLV 184

BLAST of Bhi11G001865 vs. ExPASy TrEMBL
Match: A0A6J1F238 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111441690 PE=3 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 2.2e-81
Identity = 152/184 (82.61%), Postives = 157/184 (85.33%), Query Frame = 0

Query: 1   MDKSASLMNRSPPPSPTPHRTVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLLRLW 60
           MD SA+LMNRS PP PTPHRT DFSPIEFILGL+AVI IPALVYSFFFAIKCPP LLRLW
Sbjct: 4   MDTSAALMNRSTPPHPTPHRTNDFSPIEFILGLIAVIAIPALVYSFFFAIKCPPGLLRLW 63

Query: 61  RHRSARLSGAAVTVVVRNRTPSSDVAAAVKYNKDEHCKEVSNECPVCLSAFDDGEEIRQL 120
           R RSARLSGA VTVV  NR+  SDVA   KYNKDE  KEV NECPVCLSAF DGEEIRQL
Sbjct: 64  RRRSARLSGAPVTVVAGNRSSYSDVAVTFKYNKDEVGKEVGNECPVCLSAFVDGEEIRQL 123

Query: 121 AACKHSFHVDCIDMWLYSHPNCPVCRAAVPVAVKRPINNHAPRRPAVSRSDDFQQGLPDA 180
            ACKHSFHVDCIDMW YSHPNCPVCRA + +AVKRPINN APRRPA SRSDDF QGLPDA
Sbjct: 124 IACKHSFHVDCIDMWFYSHPNCPVCRAEIAIAVKRPINNRAPRRPAASRSDDFHQGLPDA 183

Query: 181 GYLV 185
           G LV
Sbjct: 184 GNLV 187

BLAST of Bhi11G001865 vs. ExPASy TrEMBL
Match: A0A6J1J6T6 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111481888 PE=3 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 2.7e-79
Identity = 150/184 (81.52%), Postives = 154/184 (83.70%), Query Frame = 0

Query: 1   MDKSASLMNRSPPPSPTPHRTVDFSPIEFILGLVAVITIPALVYSFFFAIKCPPNLLRLW 60
           MD SA+LMNRS PP P  HRT DFSP EFILGLVAVI IPALVYSFFFAIKCPP LLRLW
Sbjct: 4   MDSSAALMNRSTPPHPMTHRTNDFSPFEFILGLVAVIAIPALVYSFFFAIKCPPGLLRLW 63

Query: 61  RHRSARLSGAAVTVVVRNRTPSSDVAAAVKYNKDEHCKEVSNECPVCLSAFDDGEEIRQL 120
           R RSARLSGA V VV  NR+  SDVAA  KYNKDE  KEV NECPVCLSAF DGEEIRQL
Sbjct: 64  RRRSARLSGAPVAVVAGNRSSYSDVAATFKYNKDEVGKEVGNECPVCLSAFVDGEEIRQL 123

Query: 121 AACKHSFHVDCIDMWLYSHPNCPVCRAAVPVAVKRPINNHAPRRPAVSRSDDFQQGLPDA 180
            ACKHSFHVDCIDMW YSHPNCPVCRA + +AVKRPINN APRRPA SRSDDF QGLPDA
Sbjct: 124 IACKHSFHVDCIDMWFYSHPNCPVCRAEIVIAVKRPINNRAPRRPAASRSDDFHQGLPDA 183

Query: 181 GYLV 185
           G LV
Sbjct: 184 GNLV 187

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT2G37580.17.5e-3440.31RING/U-box superfamily protein [more]
AT5G43420.11.5e-1334.13RING/U-box superfamily protein [more]
AT1G72220.11.9e-1355.32RING/U-box superfamily protein [more]
AT4G40070.11.9e-1330.77RING/U-box superfamily protein [more]
AT4G15975.11.9e-1359.57RING/U-box superfamily protein [more]
Match NameE-valueIdentityDescription
O809271.1e-3240.31RING-H2 finger protein ATL33 OS=Arabidopsis thaliana OX=3702 GN=ATL33 PE=2 SV=2[more]
Q9LRB72.4e-1342.57E3 ubiquitin-protein ligase EL5 OS=Oryza sativa subsp. japonica OX=39947 GN=EL5.... [more]
Q9LSW92.1e-1234.13RING-H2 finger protein ATL16 OS=Arabidopsis thaliana OX=3702 GN=ATL16 PE=2 SV=1[more]
Q8W5712.7e-1230.77RING-H2 finger protein ATL32 OS=Arabidopsis thaliana OX=3702 GN=ATL32 PE=2 SV=3[more]
Q8LFY82.7e-1255.32RING-H2 finger protein ATL54 OS=Arabidopsis thaliana OX=3702 GN=ATL54 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
XP_038905078.13.5e-102100.00RING-H2 finger protein ATL33 [Benincasa hispida][more]
KAE8651013.11.9e-9290.76hypothetical protein Csa_001104 [Cucumis sativus][more]
XP_004137943.11.9e-9290.76RING-H2 finger protein ATL33 [Cucumis sativus][more]
XP_008442561.18.2e-9189.13PREDICTED: RING-H2 finger protein ATL33 [Cucumis melo] >KAA0044082.1 RING-H2 fin... [more]
XP_022934546.14.5e-8182.61RING-H2 finger protein ATL33-like [Cucurbita moschata] >KAG6580552.1 RING-H2 fin... [more]
Match NameE-valueIdentityDescription
A0A0A0LA679.4e-9390.76RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G734330 PE... [more]
A0A5D3DN653.9e-9189.13RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3B6Q93.9e-9189.13RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103486397 PE=3 ... [more]
A0A6J1F2382.2e-8182.61RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111441690... [more]
A0A6J1J6T62.7e-7981.52RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111481888 P... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 104..145
e-value: 4.6E-6
score: 36.2
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 102..146
e-value: 4.4E-14
score: 52.6
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 104..146
score: 12.312242
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 75..152
e-value: 2.3E-20
score: 74.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 161..184
NoneNo IPR availablePANTHERPTHR45676:SF88RING-H2 FINGER PROTEIN ATL33coord: 9..184
NoneNo IPR availableCDDcd16461RING-H2_EL5_likecoord: 103..146
e-value: 8.12372E-16
score: 65.7943
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 103..152
IPR044675E3 ubiquitin-protein ligase RING1-likePANTHERPTHR45676RING-H2 FINGER PROTEIN ATL51-RELATEDcoord: 9..184

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi11M001865Bhi11M001865mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0016021 integral component of membrane