Bhi11G001312 (gene) Wax gourd (B227) v1

Overview
NameBhi11G001312
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptiontranscription termination factor MTERF4, chloroplastic
Locationchr11: 44635477 .. 44638326 (-)
RNA-Seq ExpressionBhi11G001312
SyntenyBhi11G001312
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTTTCATTTGGTGAAATTTTCATTGTTAATTCATTCAAAGCGAGAGTCGCCCCCTGCTATCCCTCTCCTTCCCCTGTTTCTCCCAAAGTCCAAATCCCATCGCCCGCCGCCGTTCACAGCTCGCCGTTCCTCCACCTGAAAGGATACCGGTATGTCTCTCTCCATCTCTCAACCCAACTCACTAGCTTGTACTCACACTTCATATCTTGATTCTCGTCCAGTTTAGGTTTCCATTGTTACACTCTCATCGTCGTCCACTTTGGGTTTTTGATACTTTCCGTTTCCACTGCGATTGCATTCATGAGAACTGGACTGAACTCTAACCTTCAATGACCACCTGTAAACATTGATCTTACAAACTAACCCCTTTTAAGATTGGGAATATTATTATGTAAGTGAATTCCGTTTTAGGGTTTATTATCTTCCTTGCCCTTACAGCTTTCTAATAGGAGATTGGGAGGATTCTATGGAGTTAGATGCTGTTTTTTCTGTTTCTGTTTCAGTTACTCTGTCTGATTGGCGCTGCTTGTTCTCTCTTTGGCAGTTTTGATTCAAATTACTGCATTATCATTTATGAGGCCAAGAAAATCTGCTAAGTTCTCCCTGGTTTTGGGCATTGCCCATCAAATTTACGAGAAATTTGGCTTATTCTGCCCCAAGATTACCAAATCCTACCTGAATCCATGTGCCCACCAATCGCATATTCAAAGCTTTTGGAGGGTTTTTCCATTAGGTGTACTGTATTCGACTCAATCCTCAAAACTTCCTGAATATGAAATGCCTACGGTCACCTGGGGAGCTGTTCAAGGGCGCAAAGAAAAACTTGTTTCCCGAGTCATAATCTGTGACTACTTAAAGAGTTTGGGGATTGTTCCTGATGAATTAGAACACTTGGAGCTGCCTTCTACGGTTGAAGTCATGAGAGAGCGTGTCGAGTTTCTTCAGAAGTTAGGATTGACGATCGATGACATTAACGAGTTCCCATTAATGCTAGGTTGTAGTGTTCGCAAAAATATAATACCTGTTCTAGGTTACTTGGAGAAAATTGGGATTCCAAGGCCAAAACTTGGAGAATTTATTAAGAATTATCCACAGGTCTTGCATGCCAGTGTCATTGTTGAGCTTGCTCCCGTCATTAAGCTTCTTCGTGGGCTCGATGTGGAGAAAGAAGATATTGGATATGTCCTCCAGAAATACCCTGAGCTTCTGGGTTTCAAGCTTGAGGGGACAATGAGTACATCTGTTGCATATCTTGTGAGTATAGGTGTTAATCCAAGAGATATTGGACCAATGGTCACACAATACCCGTACTTTTTGGGGATGAGAGTTGGAACCATGATCAAGCCCCTCGTTGAGTTTATTGTTTCTTTAGGTTTGCCAAAGAAGATAGTAGCAAGAATGCTCGAGAAGCGAGCATACATACTTGGTTATGATCTTGAAGAGACCGTGAAGCCCAATATAGATTGCTTGCTAAGCTTCGGGATTAGGAAGGAGCTGCTTCCTTCGGTGATTGCACAATATCCACAAATTCTTGGGCTGCCTCTGAAAGCGAAGTTGTCTTCTCAGCAATTTTTCTTTGACCTAAAGCTCAAGATTGATCCTGCAGGGTTTGCACAAGTGATAGAAAAAATGCCACAGATAGTCAGCCTTCACCAACACGTGATAATTAAACCGGCAGAGTTCTTTCTTGGACGCGGTATTCCGTCCTCTGATGTAGCTAAGATGATCATTAAATGTCCTCAGCTGGTCGCCTGTCGTGTAGAACTCATGAAAAACAGTTACTACTTCTTCAAGTCTGAGATGGGCAGGCCAATTAAAGAACTTGTCAACTTCCCCGAATATTTTACGTACAGTTTGGAATCGAGGATAAAACCAAGGTATGAGAGGCTGCAGAGCAAGGGAATCAGATGTTCATTAAATTGGTTTCTCAACTGCAGTGATCAAAGGTTTGAGGAGAGATTGAAGGGTGAATTCATTGAAACAGAGAGTTTGAGGCCATCATTTTGTATGGGAGGGAGATTAGAACTACCAGGGAATGAGATTATTGTGTCAGATGATGAGGTTGAGGAGGATGAGTGTGATGAAAAACTTCACAGACGTATTGTTTCTCTTTAGAAAGTTCCCCCTTTTATTTATTCAAATTTTCTTTTGCTCTGTCTGTTTGGTCTGTGAGCTATTTTTGGTTTGATATTTTTTAGAACCTAAAAGACGAAAAAAAATGGTGAAAATACACATTGTATACTTATTCTGAAAAAAGGATTTTCAAAAAAACTCTTAAAATAGTGTACTTTGATACACTTGCAAAACTTTTCGTTAATGGGACTTTTGTTTAAAACCGTAGACGGATTTTCACAAAGATATAACAAGCACATGGAACCATCTCTTTTTCTTTCTGTCAAGAAAAAATATGGTAGACTAACTTACTTATACTTATATTTATAGCTGTTAATGAAAGAGCCCAATGCAAAACATTATTTTCTCATTTCCAAGAGAGAGAATCCTATAGGGTAGTGCGTGTCAATGAAATTATTCCACCCAAGATCTACATGCAGAAAAATTATTTTAACATTCAACTGCTCCAATTGAAGATTATAAGCAGCTGTCAATGAAATGATCAGAACTCTGATTTGGGGTGTCTTAACTGGAAGGAAGATCCGAGTGTACCCCTTTGGAGGTTGGAACTAGTCTCGCGTTATTCCGACTATGATGTCCATGCTTTCCTTAAGTTTTATAGATTCACTTGCAGCCTAACCAACTTTTGGTTAATGTTCGATGCACTCGATCCCAAAGTTTCATTGATATTATTATTTTGTCAACATGAGCATATCTAAATGCCATGAAATATG

mRNA sequence

TTTTTTCATTTGGTGAAATTTTCATTGTTAATTCATTCAAAGCGAGAGTCGCCCCCTGCTATCCCTCTCCTTCCCCTGTTTCTCCCAAAGTCCAAATCCCATCGCCCGCCGCCGTTCACAGCTCGCCGTTCCTCCACCTGAAAGGATACCGGTATGTCTCTCTCCATCTCTCAACCCAACTCACTAGCTTGTACTCACACTTCATATCTTGATTCTCGTCCAGTTTAGGTTTCCATTGTTACACTCTCATCGTCGTCCACTTTGGGTTTTTGATACTTTCCGTTTCCACTGCGATTGCATTCATGAGAACTGGACTGAACTCTAACCTTCAATGACCACCTGTAAACATTGATCTTACAAACTAACCCCTTTTAAGATTGGGAATATTATTATTTTTGATTCAAATTACTGCATTATCATTTATGAGGCCAAGAAAATCTGCTAAGTTCTCCCTGGTTTTGGGCATTGCCCATCAAATTTACGAGAAATTTGGCTTATTCTGCCCCAAGATTACCAAATCCTACCTGAATCCATGTGCCCACCAATCGCATATTCAAAGCTTTTGGAGGGTTTTTCCATTAGGTGTACTGTATTCGACTCAATCCTCAAAACTTCCTGAATATGAAATGCCTACGGTCACCTGGGGAGCTGTTCAAGGGCGCAAAGAAAAACTTGTTTCCCGAGTCATAATCTGTGACTACTTAAAGAGTTTGGGGATTGTTCCTGATGAATTAGAACACTTGGAGCTGCCTTCTACGGTTGAAGTCATGAGAGAGCGTGTCGAGTTTCTTCAGAAGTTAGGATTGACGATCGATGACATTAACGAGTTCCCATTAATGCTAGGTTGTAGTGTTCGCAAAAATATAATACCTGTTCTAGGTTACTTGGAGAAAATTGGGATTCCAAGGCCAAAACTTGGAGAATTTATTAAGAATTATCCACAGGTCTTGCATGCCAGTGTCATTGTTGAGCTTGCTCCCGTCATTAAGCTTCTTCGTGGGCTCGATGTGGAGAAAGAAGATATTGGATATGTCCTCCAGAAATACCCTGAGCTTCTGGGTTTCAAGCTTGAGGGGACAATGAGTACATCTGTTGCATATCTTGTGAGTATAGGTGTTAATCCAAGAGATATTGGACCAATGGTCACACAATACCCGTACTTTTTGGGGATGAGAGTTGGAACCATGATCAAGCCCCTCGTTGAGTTTATTGTTTCTTTAGGTTTGCCAAAGAAGATAGTAGCAAGAATGCTCGAGAAGCGAGCATACATACTTGGTTATGATCTTGAAGAGACCGTGAAGCCCAATATAGATTGCTTGCTAAGCTTCGGGATTAGGAAGGAGCTGCTTCCTTCGGTGATTGCACAATATCCACAAATTCTTGGGCTGCCTCTGAAAGCGAAGTTGTCTTCTCAGCAATTTTTCTTTGACCTAAAGCTCAAGATTGATCCTGCAGGGTTTGCACAAGTGATAGAAAAAATGCCACAGATAGTCAGCCTTCACCAACACGTGATAATTAAACCGGCAGAGTTCTTTCTTGGACGCGGTATTCCGTCCTCTGATGTAGCTAAGATGATCATTAAATGTCCTCAGCTGGTCGCCTGTCGTGTAGAACTCATGAAAAACAGTTACTACTTCTTCAAGTCTGAGATGGGCAGGCCAATTAAAGAACTTGTCAACTTCCCCGAATATTTTACGTACAGTTTGGAATCGAGGATAAAACCAAGGTATGAGAGGCTGCAGAGCAAGGGAATCAGATGTTCATTAAATTGGTTTCTCAACTGCAGTGATCAAAGGTTTGAGGAGAGATTGAAGGGTGAATTCATTGAAACAGAGAGTTTGAGGCCATCATTTTGTATGGGAGGGAGATTAGAACTACCAGGGAATGAGATTATTGTGTCAGATGATGAGGTTGAGGAGGATGAGTGTGATGAAAAACTTCACAGACGTATTGTTTCTCTTTAGAAAGTTCCCCCTTTTATTTATTCAAATTTTCTTTTGCTCTGTCTGTTTGGTCTGTGAGCTATTTTTGGTTTGATATTTTTTAGAACCTAAAAGACGAAAAAAAATGGTGAAAATACACATTGTATACTTATTCTGAAAAAAGGATTTTCAAAAAAACTCTTAAAATAGTGTACTTTGATACACTTGCAAAACTTTTCGTTAATGGGACTTTTGTTTAAAACCGTAGACGGATTTTCACAAAGATATAACAAGCACATGGAACCATCTCTTTTTCTTTCTGTCAAGAAAAAATATGGTAGACTAACTTACTTATACTTATATTTATAGCTGTTAATGAAAGAGCCCAATGCAAAACATTATTTTCTCATTTCCAAGAGAGAGAATCCTATAGGGTAGTGCGTGTCAATGAAATTATTCCACCCAAGATCTACATGCAGAAAAATTATTTTAACATTCAACTGCTCCAATTGAAGATTATAAGCAGCTGTCAATGAAATGATCAGAACTCTGATTTGGGGTGTCTTAACTGGAAGGAAGATCCGAGTGTACCCCTTTGGAGGTTGGAACTAGTCTCGCGTTATTCCGACTATGATGTCCATGCTTTCCTTAAGTTTTATAGATTCACTTGCAGCCTAACCAACTTTTGGTTAATGTTCGATGCACTCGATCCCAAAGTTTCATTGATATTATTATTTTGTCAACATGAGCATATCTAAATGCCATGAAATATG

Coding sequence (CDS)

ATGAGGCCAAGAAAATCTGCTAAGTTCTCCCTGGTTTTGGGCATTGCCCATCAAATTTACGAGAAATTTGGCTTATTCTGCCCCAAGATTACCAAATCCTACCTGAATCCATGTGCCCACCAATCGCATATTCAAAGCTTTTGGAGGGTTTTTCCATTAGGTGTACTGTATTCGACTCAATCCTCAAAACTTCCTGAATATGAAATGCCTACGGTCACCTGGGGAGCTGTTCAAGGGCGCAAAGAAAAACTTGTTTCCCGAGTCATAATCTGTGACTACTTAAAGAGTTTGGGGATTGTTCCTGATGAATTAGAACACTTGGAGCTGCCTTCTACGGTTGAAGTCATGAGAGAGCGTGTCGAGTTTCTTCAGAAGTTAGGATTGACGATCGATGACATTAACGAGTTCCCATTAATGCTAGGTTGTAGTGTTCGCAAAAATATAATACCTGTTCTAGGTTACTTGGAGAAAATTGGGATTCCAAGGCCAAAACTTGGAGAATTTATTAAGAATTATCCACAGGTCTTGCATGCCAGTGTCATTGTTGAGCTTGCTCCCGTCATTAAGCTTCTTCGTGGGCTCGATGTGGAGAAAGAAGATATTGGATATGTCCTCCAGAAATACCCTGAGCTTCTGGGTTTCAAGCTTGAGGGGACAATGAGTACATCTGTTGCATATCTTGTGAGTATAGGTGTTAATCCAAGAGATATTGGACCAATGGTCACACAATACCCGTACTTTTTGGGGATGAGAGTTGGAACCATGATCAAGCCCCTCGTTGAGTTTATTGTTTCTTTAGGTTTGCCAAAGAAGATAGTAGCAAGAATGCTCGAGAAGCGAGCATACATACTTGGTTATGATCTTGAAGAGACCGTGAAGCCCAATATAGATTGCTTGCTAAGCTTCGGGATTAGGAAGGAGCTGCTTCCTTCGGTGATTGCACAATATCCACAAATTCTTGGGCTGCCTCTGAAAGCGAAGTTGTCTTCTCAGCAATTTTTCTTTGACCTAAAGCTCAAGATTGATCCTGCAGGGTTTGCACAAGTGATAGAAAAAATGCCACAGATAGTCAGCCTTCACCAACACGTGATAATTAAACCGGCAGAGTTCTTTCTTGGACGCGGTATTCCGTCCTCTGATGTAGCTAAGATGATCATTAAATGTCCTCAGCTGGTCGCCTGTCGTGTAGAACTCATGAAAAACAGTTACTACTTCTTCAAGTCTGAGATGGGCAGGCCAATTAAAGAACTTGTCAACTTCCCCGAATATTTTACGTACAGTTTGGAATCGAGGATAAAACCAAGGTATGAGAGGCTGCAGAGCAAGGGAATCAGATGTTCATTAAATTGGTTTCTCAACTGCAGTGATCAAAGGTTTGAGGAGAGATTGAAGGGTGAATTCATTGAAACAGAGAGTTTGAGGCCATCATTTTGTATGGGAGGGAGATTAGAACTACCAGGGAATGAGATTATTGTGTCAGATGATGAGGTTGAGGAGGATGAGTGTGATGAAAAACTTCACAGACGTATTGTTTCTCTTTAG

Protein sequence

MRPRKSAKFSLVLGIAHQIYEKFGLFCPKITKSYLNPCAHQSHIQSFWRVFPLGVLYSTQSSKLPEYEMPTVTWGAVQGRKEKLVSRVIICDYLKSLGIVPDELEHLELPSTVEVMRERVEFLQKLGLTIDDINEFPLMLGCSVRKNIIPVLGYLEKIGIPRPKLGEFIKNYPQVLHASVIVELAPVIKLLRGLDVEKEDIGYVLQKYPELLGFKLEGTMSTSVAYLVSIGVNPRDIGPMVTQYPYFLGMRVGTMIKPLVEFIVSLGLPKKIVARMLEKRAYILGYDLEETVKPNIDCLLSFGIRKELLPSVIAQYPQILGLPLKAKLSSQQFFFDLKLKIDPAGFAQVIEKMPQIVSLHQHVIIKPAEFFLGRGIPSSDVAKMIIKCPQLVACRVELMKNSYYFFKSEMGRPIKELVNFPEYFTYSLESRIKPRYERLQSKGIRCSLNWFLNCSDQRFEERLKGEFIETESLRPSFCMGGRLELPGNEIIVSDDEVEEDECDEKLHRRIVSL
Homology
[Close]
BLAST of Bhi11G001312 vs. TAIR 10
Match: AT2G44020.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 748.0 bits (1930), Expect = 4.9e-216
Identity = 359/482 (74.48%), Postives = 426/482 (88.38%), Query Frame = 0 

Query: 32  KSYLNPCAHQSHIQSFWRVFPLGVLYSTQSSKLPEYEMPTVTWGAVQGRKEKLVSRVIIC 91
           K+ +NP  +    Q+   +F +   Y+TQSSK PEYEMPTVTWG +QG+KEKLV+RV IC
Sbjct: 29  KTLINPDPNIPQFQNPCSIFRI-AHYATQSSKFPEYEMPTVTWGVIQGKKEKLVNRVKIC 88

Query: 92  DYLKSLGIVPDELEHLELPSTVEVMRERVEFLQKLGLTIDDINEFPLMLGCSVRKNIIPV 151
           DYLK LGI+ DELE +ELPST+EVM ERVEFLQKLGLTIDDINE+PLMLGCSVRKN+IPV
Sbjct: 89  DYLKGLGIITDELESIELPSTIEVMCERVEFLQKLGLTIDDINEYPLMLGCSVRKNLIPV 148

Query: 152 LGYLEKIGIPRPKLGEFIKNYPQVLHASVIVELAPVIKLLRGLDVEKEDIGYVLQKYPEL 211
           L YLEKIGI R KLGEF+KNYPQVLHASV+VELAPV+K LRGLDVEK+D+GYVL KYPEL
Sbjct: 149 LAYLEKIGISRSKLGEFVKNYPQVLHASVVVELAPVVKFLRGLDVEKQDLGYVLMKYPEL 208

Query: 212 LGFKLEGTMSTSVAYLVSIGVNPRDIGPMVTQYPYFLGMRVGTMIKPLVEFIVSLGLPKK 271
           LGFKLEGTMSTSVAYLVSIGV+PRDIGPMVTQYPY LGMRVGTMIKPLV++++S+GLPKK
Sbjct: 209 LGFKLEGTMSTSVAYLVSIGVSPRDIGPMVTQYPYLLGMRVGTMIKPLVDYLISIGLPKK 268

Query: 272 IVARMLEKRAYILGYDLEETVKPNIDCLLSFGIRKELLPSVIAQYPQILGLPLKAKLSSQ 331
           IVARMLEKR+YI+GY+LEETVKPN+DCL+SFG++KELLP +IAQYPQILGLP+KAK+S+Q
Sbjct: 269 IVARMLEKRSYIVGYNLEETVKPNVDCLISFGVKKELLPLLIAQYPQILGLPVKAKMSTQ 328

Query: 332 QFFFDLKLKIDPAGFAQVIEKMPQIVSLHQHVIIKPAEFFLGRGIPSSDVAKMIIKCPQL 391
           Q+FF LKLKIDP GFA+V+EKMPQIVSL Q+VI+KP EF LGR     D+AKM+++CPQ+
Sbjct: 329 QYFFSLKLKIDPEGFARVVEKMPQIVSLKQNVIMKPIEFLLGRAFQVEDIAKMVVRCPQI 388

Query: 392 VACRVELMKNSYYFFKSEMGRPIKELVNFPEYFTYSLESRIKPRYERLQSKGIRCSLNWF 451
           +  RVELMKNSYYF+K+EMGRP+KELV +PEYFTYSLESRIKPRY++LQSKGIR SLNWF
Sbjct: 389 LCSRVELMKNSYYFYKTEMGRPMKELVEYPEYFTYSLESRIKPRYQKLQSKGIRSSLNWF 448

Query: 452 LNCSDQRFEERLKGEFIETESLRPSFCMGGRLELPGNEIIVSDDEVEEDECDEKLHRRIV 511
           LNCSDQRFEERL+G FI+ ++  P+F MGG+LE+PG EI+  ++E E D  DE L+RR +
Sbjct: 449 LNCSDQRFEERLQGNFIDPDTEGPTFDMGGKLEMPGGEIVTDEEEDESD--DEVLYRRTL 507

Query: 512 SL 514
           +L
Sbjct: 509 TL 507

[Close]
BLAST of Bhi11G001312 vs. TAIR 10
Match: AT4G02990.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 449.1 bits (1154), Expect = 4.7e-126
Identity = 214/428 (50.00%), Postives = 312/428 (72.90%), Query Frame = 0 

Query: 81  KEKLVSRVIICDYLKSLGIVPDELEHLELPSTVEVMRERVEFLQKLGLTIDDINEFPLML 140
           KEK  +R  + ++L+ +GIVPDEL+ LELP T +VM+ERVEFL KLGLTI+DIN +PL+L
Sbjct: 112 KEKAANRAKVYEFLRGIGIVPDELDGLELPVTADVMKERVEFLHKLGLTIEDINNYPLVL 171

Query: 141 GCSVRKNIIPVLGYLEKIGIPRPKLGEFIKNYPQVLHASVIVELAPVIKLLRGLDVEKED 200
           GCSV+KN++PVL YL K+G+ +    EF++ YPQVLH+SV+++LAPV+K L+GLD++  D
Sbjct: 172 GCSVKKNMVPVLDYLGKLGVRKSTFTEFLRRYPQVLHSSVVIDLAPVVKYLQGLDIKPSD 231

Query: 201 IGYVLQKYPELLGFKLEGTMSTSVAYLVSIGVNPRDIGPMVTQYPYFLGMRVGTMIKPLV 260
           +  VL++YPE+LGFKLEGTMSTSVAYLV IGV  R+IG ++T+YP  LGMRV  +IKPLV
Sbjct: 232 VPRVLERYPEVLGFKLEGTMSTSVAYLVGIGVARREIGGILTRYPEILGMRVARIIKPLV 291

Query: 261 EFIVSLGLPKKIVARMLEKRAYILGYDLEETVKPNIDCLLSFGIRKELLPSVIAQYPQIL 320
           E++  LG+P+   AR++EKR +ILG++L++TVKPN+  L  F +R+  LPS+IAQYP+I+
Sbjct: 292 EYLEVLGIPRLAAARLIEKRPHILGFELDDTVKPNVQILQDFNVRETSLPSIIAQYPEII 351

Query: 321 GLPLKAKLSSQQFFFDLKLKIDPAGFAQVIEKMPQIVSLHQHVIIKPAEFFLGRGIPSSD 380
           G+ LK KL +Q+      + ++P     +IE+MPQ VSL +  ++K  +F    G     
Sbjct: 352 GIDLKPKLDTQRKLLCSAIHLNPEDLGSLIERMPQFVSLSESPMLKHIDFLTKCGFSIDQ 411

Query: 381 VAKMIIKCPQLVACRVELMKNSYYFFKSEMGRPIKELVNFPEYFTYSLESRIKPRYERLQ 440
             +M+I CPQ++A  + +MK S+ +F+ EM RP+++LV+FP +FTY LES +KPR++++ 
Sbjct: 412 TREMVIGCPQVLALNLGIMKLSFEYFQKEMKRPLQDLVDFPAFFTYGLESTVKPRHKKII 471

Query: 441 SKGIRCSLNWFLNCSDQRFEERLKGEFIETESLR---PSFCMGGRLELPGNEIIVSDDEV 500
            KGI+CSL W LNCSD++FE+R+  + I+ E +     SF M   ++    E   S+ E 
Sbjct: 472 KKGIKCSLAWMLNCSDEKFEQRMSYDTIDIEEVETDPSSFDMNTLMQPEREEESDSEYEE 531

Query: 501 EEDECDEK 506
           EED+ DE+
Sbjct: 532 EEDDDDEE 539

[Close]
BLAST of Bhi11G001312 vs. TAIR 10
Match: AT2G21710.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 156.8 bits (395), Expect = 4.8e-38
Identity = 92/370 (24.86%), Postives = 182/370 (49.19%), Query Frame = 0 

Query: 145 RKNIIPVLGYLEKIGIPRPKLGEFIKNYPQVLHASVIVELAPVIKLLRGLDVEKEDIGYV 204
           R+ +  ++ YLE  G+ R  +G  +   P++L  S + E+   +     + + + D G +
Sbjct: 278 REELNEIVEYLESNGVRRDWMGYVVGRCPELLSFS-MEEVKSRVDFFLKMGMNQNDFGTM 337

Query: 205 LQKYPELLGFKLEGTMSTSVAYLVSIGVNPRDIGPMVTQYPYFLGMRVGTMIKPLVEFIV 264
           +  YP+++GF     M   + YL   G++  ++G ++   P+ +G  +    KPLV++  
Sbjct: 338 VYDYPKIIGFFSFQVMEKKINYLKEFGLSTEEVGRLLAYKPHLMGCSIEERWKPLVKYFY 397

Query: 265 SLGLPKKIVARMLEKRAYILGYDLEETVKPNIDCLLSFGIRKELLPSVIAQYPQILGLPL 324
            LG+PK+ + R+L  +  +   DLE+T+ P +  L   GI  E + +++ ++P +L   L
Sbjct: 398 YLGIPKEGMKRILVVKPILYCIDLEKTIAPKVRFLQEMGIPNEAIGNMLVKFPSLLTNSL 457

Query: 325 KAKLSSQQFFFDLKLKIDPAGFAQVIEKMPQIVSLHQHVIIKP-AEFFLGRGIPSSDVAK 384
             K+     F   +  +      +VI   P ++       ++P   +++  GI    + +
Sbjct: 458 YKKIRPVVIFLLTRAGVTQKDIGKVIAMDPALLGCSIGTKLEPNMRYYISLGIRFYQLGE 517

Query: 385 MIIKCPQLVACRVELMKNSYYFFKSEMGRPIKELVNFPEYFTYSLESRIKPRYERLQSKG 444
           MI   P L+   V+ ++  Y + +  M RP+++L+ FP +F+YSLE RI PR+  +    
Sbjct: 518 MIADFPMLLRYNVDNLRPKYRYLRRTMIRPLQDLIEFPRFFSYSLERRIIPRHTIMVENR 577

Query: 445 IRCSLNWFLNCSDQRFEERLKGEF---------IETESLRPSFCMGGRLELPGNEIIVSD 504
           +   L + L C+D+ FE R++ +          +++E  +PS        +   EI  SD
Sbjct: 578 VNFKLRYMLACTDEEFERRVRDKVERRERFEAGLDSEDSQPS-----DENISDQEIAFSD 637

[Close]
BLAST of Bhi11G001312 vs. TAIR 10
Match: AT1G78930.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 137.5 bits (345), Expect = 3.0e-32
Identity = 94/368 (25.54%), Postives = 180/368 (48.91%), Query Frame = 0 

Query: 96  SLGIVPDELEHLELPSTVEVMRERVEFLQKLGLTIDDINE-FPLMLGCSVRKNIIPVLGY 155
           S+ I  D  + L     +E  R  ++ L  +  +   + E FP +L  S   ++ P++ +
Sbjct: 211 SIPIDEDVQQTLSFFEKIEARRGGLDMLGSVDASFRFLLESFPRLLLLSEENDMKPMVEF 270

Query: 156 LEKIGIPRPKLGEFIKNYPQVLHASVIVELAPVIKLLRGLDVEKEDIGYVLQKYPELLGF 215
           LE IGIP+  LG+ +  YP ++          V   +  + V  +D G +L KYP +L  
Sbjct: 271 LESIGIPKYCLGKVLLLYPPIMLGKTEEIKRRVATAMEKVSVVNKDSGKLLLKYPWILSP 330

Query: 216 KLEGTMSTSVAYLVSIGVNPRDIGPMVTQYPYFLGMRVGTMIKPLVEFIVSLGLPKKIVA 275
            ++   S   ++  S  V   DI   + ++P  LG     M   + EF   LG+  K + 
Sbjct: 331 SIQENYSHIGSFFYSESVLKMDIDHAIRRWPLLLGCSASNMEMMVKEF-DKLGVRDKRMG 390

Query: 276 RMLEKRAYILGYDLEETVKPNIDCLL-SFGIRKELLPSVIAQYPQILGLPLKAKLSSQQF 335
           +++ K   +L    +E +K  + C L   G +KE++  ++ + P+I G  ++  L  +  
Sbjct: 391 KVIPKMPQLLLCKPQEFLK--VVCFLEDLGFQKEIVGQILCRCPEIFGCSIEKTLQKKLI 450

Query: 336 FFDLKLKIDPAGFAQVIEKMPQIVSLHQHVIIKP-AEFFLGRGIPSSDVAKMIIKCPQLV 395
           F   +  +    F ++I+K P+ +       + P  ++ +  GI   ++A MI K   ++
Sbjct: 451 FL-TRFGVSTTHFPRIIKKYPEFLIYDADKTVLPRLKYLMEIGISEREIAFMIRKFSPIL 510

Query: 396 ACRVE-LMKNSYYFFKSEMGRPIKELVNFPEYFTYSLESRIKPRYERLQSKGIRCSLNWF 455
              ++ +++  + F  + M +P++E++ +P YF+YSLE RIKPR+  L+ + I C+L   
Sbjct: 511 GYSIDKVLRPKFEFLVNSMEKPVREVIEYPRYFSYSLEKRIKPRFRVLKGRNIECTLQEM 570

Query: 456 LNCSDQRF 460
           L  +D+ F
Sbjct: 571 LGKNDEEF 574

[Close]
BLAST of Bhi11G001312 vs. TAIR 10
Match: AT4G38160.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 123.2 bits (308), Expect = 5.9e-28
Identity = 73/322 (22.67%), Postives = 159/322 (49.38%), Query Frame = 0 

Query: 147 NIIPVLGYLEKIGIPRPKLGEFIKNYPQVLHASVIVELAPVIKLLRGLDVEKEDIGYVLQ 206
           N   ++ +    G   P + + ++   Q+  A   V       L   + +++  + Y++ 
Sbjct: 5   NTSSIMWFFRDKGFDDPSIDKMLRKCKQLEKAQSDVASENWDYLSNIVGIQERKLPYIVS 64

Query: 207 KYPELLGFKLEGTMSTSVAYLVSIGVNPRDIGPMVTQYPYFLGMRVGTMIKPLVEFIVSL 266
           + P++L  +L+  +   V  L S+G NPR++   +T++P  L   V   + PL+ F  +L
Sbjct: 65  RCPKILTLRLDERLIPMVECLSSLGRNPREVASAITKFPPILSHSVEEKLCPLLAFFQAL 124

Query: 267 GLPKKIVARMLEKRAYILGYDLEETVKPNIDCLLSFGIRKE-LLPSVIAQYPQILGLPLK 326
           G+P+  + +M+     ++ Y ++  +   +  L S G+ ++ ++  V+ + P ++G  + 
Sbjct: 125 GVPETQLGKMILFNPRLISYSIDTKLTVIVSFLASLGLDQDGMIGKVLVKNPFLMGYSVD 184

Query: 327 AKLSSQQFFFDLKLKIDPAGFAQVIEKMPQIVSLHQHVIIKPAEFFLGR-GIPSSDVAKM 386
            +L     F    + +   G   V+   PQ++    + I+KP   +L   G   S +A M
Sbjct: 185 KRLRPTTEFLKSSVGLSEDGIKSVVMNFPQLLCRDVNKILKPNYDYLKECGFGDSQIATM 244

Query: 387 IIKCPQLVACRVELMKNSYY----FFKSEMGRPIKELVNFPEYFTYSLESRIKPRYERLQ 446
           +   PQ++   ++ +KNS      F    MGR + E+ ++PE+F + L+ +++ R++ ++
Sbjct: 245 VTGYPQIL---IKSVKNSLQPRIRFLVQVMGRGMDEVASYPEFFHHGLKKKVESRFKLVK 304

Query: 447 SKGIRCSLNWFLNCSDQRFEER 463
              I CSL   L+C+ ++F E+
Sbjct: 305 KNNIDCSLREMLDCNTKKFHEK 323

[Close]
BLAST of Bhi11G001312 vs. ExPASy Swiss-Prot
Match: Q9ZT96 (Transcription termination factor MTERF4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF4 PE=1 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 6.6e-125
Identity = 214/428 (50.00%), Postives = 312/428 (72.90%), Query Frame = 0 

Query: 81  KEKLVSRVIICDYLKSLGIVPDELEHLELPSTVEVMRERVEFLQKLGLTIDDINEFPLML 140
           KEK  +R  + ++L+ +GIVPDEL+ LELP T +VM+ERVEFL KLGLTI+DIN +PL+L
Sbjct: 112 KEKAANRAKVYEFLRGIGIVPDELDGLELPVTADVMKERVEFLHKLGLTIEDINNYPLVL 171

Query: 141 GCSVRKNIIPVLGYLEKIGIPRPKLGEFIKNYPQVLHASVIVELAPVIKLLRGLDVEKED 200
           GCSV+KN++PVL YL K+G+ +    EF++ YPQVLH+SV+++LAPV+K L+GLD++  D
Sbjct: 172 GCSVKKNMVPVLDYLGKLGVRKSTFTEFLRRYPQVLHSSVVIDLAPVVKYLQGLDIKPSD 231

Query: 201 IGYVLQKYPELLGFKLEGTMSTSVAYLVSIGVNPRDIGPMVTQYPYFLGMRVGTMIKPLV 260
           +  VL++YPE+LGFKLEGTMSTSVAYLV IGV  R+IG ++T+YP  LGMRV  +IKPLV
Sbjct: 232 VPRVLERYPEVLGFKLEGTMSTSVAYLVGIGVARREIGGILTRYPEILGMRVARIIKPLV 291

Query: 261 EFIVSLGLPKKIVARMLEKRAYILGYDLEETVKPNIDCLLSFGIRKELLPSVIAQYPQIL 320
           E++  LG+P+   AR++EKR +ILG++L++TVKPN+  L  F +R+  LPS+IAQYP+I+
Sbjct: 292 EYLEVLGIPRLAAARLIEKRPHILGFELDDTVKPNVQILQDFNVRETSLPSIIAQYPEII 351

Query: 321 GLPLKAKLSSQQFFFDLKLKIDPAGFAQVIEKMPQIVSLHQHVIIKPAEFFLGRGIPSSD 380
           G+ LK KL +Q+      + ++P     +IE+MPQ VSL +  ++K  +F    G     
Sbjct: 352 GIDLKPKLDTQRKLLCSAIHLNPEDLGSLIERMPQFVSLSESPMLKHIDFLTKCGFSIDQ 411

Query: 381 VAKMIIKCPQLVACRVELMKNSYYFFKSEMGRPIKELVNFPEYFTYSLESRIKPRYERLQ 440
             +M+I CPQ++A  + +MK S+ +F+ EM RP+++LV+FP +FTY LES +KPR++++ 
Sbjct: 412 TREMVIGCPQVLALNLGIMKLSFEYFQKEMKRPLQDLVDFPAFFTYGLESTVKPRHKKII 471

Query: 441 SKGIRCSLNWFLNCSDQRFEERLKGEFIETESLR---PSFCMGGRLELPGNEIIVSDDEV 500
            KGI+CSL W LNCSD++FE+R+  + I+ E +     SF M   ++    E   S+ E 
Sbjct: 472 KKGIKCSLAWMLNCSDEKFEQRMSYDTIDIEEVETDPSSFDMNTLMQPEREEESDSEYEE 531

Query: 501 EEDECDEK 506
           EED+ DE+
Sbjct: 532 EEDDDDEE 539

[Close]
BLAST of Bhi11G001312 vs. ExPASy Swiss-Prot
Match: B6TGN4 (Transcription termination factor MTERF4, chloroplastic OS=Zea mays OX=4577 GN=MTERF4 PE=2 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 2.7e-118
Identity = 204/424 (48.11%), Postives = 295/424 (69.58%), Query Frame = 0 

Query: 81  KEKLVSRVIICDYLKSLGIVPDELEHLELPSTVEVMRERVEFLQKLGLTIDDINEFPLML 140
           +++   R  +  +L SLG+ P EL  LELP TV+VMRER EFL  LGLT +D+  +PL L
Sbjct: 68  RDRATRRADVDAFLTSLGVDPGELAGLELPVTVDVMRERAEFLGSLGLTQEDLAAYPLAL 127

Query: 141 GCSVRKNIIPVLGYLEKIGIPRPKLGEFIKNYPQVLHASVIVELAPVIKLLRGLDVEKED 200
           GCSVRKN++PVL YL K+G+ R  L + ++ YPQVLHASV+V+LAPV+K L+G+DV   D
Sbjct: 128 GCSVRKNMVPVLDYLGKLGVRRDALPDLLRRYPQVLHASVVVDLAPVVKYLQGMDVRPTD 187

Query: 201 IGYVLQKYPELLGFKLEGTMSTSVAYLVSIGVNPRDIGPMVTQYPYFLGMRVGTMIKPLV 260
           +  VL++YPELLGFKLEGTMSTSVAYLV IGV  R +G ++T++P  LGMRVG +IKP V
Sbjct: 188 VPRVLERYPELLGFKLEGTMSTSVAYLVGIGVGRRQVGSVITRFPEVLGMRVGKIIKPFV 247

Query: 261 EFIVSLGLPKKIVARMLEKRAYILGYDLEETVKPNIDCLLSFGIRKELLPSVIAQYPQIL 320
           E +  +GL +  +AR++EK+ Y+LG+ L+E VKPNI+ L+  G+RKE L S++ QYP +L
Sbjct: 248 EHLEGIGLQRLAIARIIEKKPYVLGFGLQEKVKPNIEALVDIGVRKEALASIVMQYPDVL 307

Query: 321 GLPLKAKLSSQQFFFDLKLKIDPAGFAQVIEKMPQIVSLHQHVIIKPAEFFLGRGIPSSD 380
           GL L+ KL +QQ  F+  + +    F +V+E+MPQ +SL +  ++K   F    G   S 
Sbjct: 308 GLELRDKLVAQQSLFESSILVSREDFGRVLERMPQAISLGRAAVLKHVNFLTACGFMLSQ 367

Query: 381 VAKMIIKCPQLVACRVELMKNSYYFFKSEMGRPIKELVNFPEYFTYSLESRIKPRYERLQ 440
           V+KM++ CPQL+A  +++M+ ++ +FK+EM R ++ELV FP +FTY +ES ++PR+E + 
Sbjct: 368 VSKMVVACPQLLALNIDIMRMNFEYFKNEMERDLEELVEFPAFFTYGIESTVRPRHEMVS 427

Query: 441 SKGIRCSLNWFLNCSDQRFEERLKGEFIETESLRPSFCMGGRLELPGNEIIVSDDEVEED 500
            KG+ CSL W LNCSD +F+ER+K + I  E +              +++  S DEVE +
Sbjct: 428 RKGLTCSLAWLLNCSDAKFDERMKYDTIGVEEMERE---------DSSDMNASVDEVESE 482

Query: 501 ECDE 505
           E ++
Sbjct: 488 EYED 482

[Close]
BLAST of Bhi11G001312 vs. ExPASy Swiss-Prot
Match: Q6AUK6 (Transcription termination factor MTERF4, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0404000 PE=2 SV=1)

HSP 1 Score: 426.4 bits (1095), Expect = 4.6e-118
Identity = 208/414 (50.24%), Postives = 293/414 (70.77%), Query Frame = 0 

Query: 93  YLKSLGIVPDELEHLELPSTVEVMRERVEFLQKLGLTIDDINEFPLMLGCSVRKNIIPVL 152
           +L SLG+ P EL  LELP+TV+VMRERVEFL  L L+ +D+  +PL LGCSVRKN++PVL
Sbjct: 89  FLASLGVDPGELAGLELPATVDVMRERVEFLHSLDLSNEDLAAYPLALGCSVRKNMVPVL 148

Query: 153 GYLEKIGIPRPKLGEFIKNYPQVLHASVIVELAPVIKLLRGLDVEKEDIGYVLQKYPELL 212
            YL K+G+ +  L + ++ YPQVLHASV+V+LAPV+K L+G+DV   D+  VL++YPELL
Sbjct: 149 DYLGKLGVRQDALPDLLRRYPQVLHASVVVDLAPVVKYLQGMDVRPHDVPRVLERYPELL 208

Query: 213 GFKLEGTMSTSVAYLVSIGVNPRDIGPMVTQYPYFLGMRVGTMIKPLVEFIVSLGLPKKI 272
           GFKLEGTMSTS+AYLV IGV  R +G ++T++P  LGMRVG +IKP VE +  +GL +  
Sbjct: 209 GFKLEGTMSTSIAYLVGIGVARRQVGSVITRFPEVLGMRVGKIIKPFVEHLEGIGLQRLA 268

Query: 273 VARMLEKRAYILGYDLEETVKPNIDCLLSFGIRKELLPSVIAQYPQILGLPLKAKLSSQQ 332
           +AR++EK+ Y+LG+ LE+ VKPNI+ LL FG+RKE L  ++AQYP ILG+ L+ KL++QQ
Sbjct: 269 IARIIEKKPYVLGFGLEDKVKPNIEALLEFGVRKEALAFIVAQYPDILGIELRDKLATQQ 328

Query: 333 FFFDLKLKIDPAGFAQVIEKMPQIVSLHQHVIIKPAEFFLGRGIPSSDVAKMIIKCPQLV 392
             F+  + +    F +VIE+MPQ +SL +  ++K   F    G   S V+KM++ CPQL+
Sbjct: 329 SLFESSILVSSEDFGRVIERMPQAISLGRTAVLKHVNFLTSCGFLLSQVSKMVVACPQLL 388

Query: 393 ACRVELMKNSYYFFKSEMGRPIKELVNFPEYFTYSLESRIKPRYERLQSKGIRCSLNWFL 452
           A  +++MK S+ +F++EM R ++ELV FP +FTY LES ++PR+E +  KG  CSL W L
Sbjct: 389 ALNMDIMKMSFEYFQNEMERDLEELVEFPAFFTYGLESTVRPRHEMVAKKGFTCSLAWLL 448

Query: 453 NCSDQRFEERLKGEFIETESLR--PSFCMGGRLELPGNEIIVSDDEVEEDECDE 505
           NCSD +F+ER+K + I  E +    SF      E         +DEVE+++ DE
Sbjct: 449 NCSDAKFDERMKYDTIGIEEMEVDNSFDTNTLSER-------VEDEVEDEDLDE 495

[Close]
BLAST of Bhi11G001312 vs. ExPASy Swiss-Prot
Match: F4IHL3 (Transcription termination factor MTERF2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF2 PE=3 SV=1)

HSP 1 Score: 156.8 bits (395), Expect = 6.8e-37
Identity = 92/370 (24.86%), Postives = 182/370 (49.19%), Query Frame = 0 

Query: 145 RKNIIPVLGYLEKIGIPRPKLGEFIKNYPQVLHASVIVELAPVIKLLRGLDVEKEDIGYV 204
           R+ +  ++ YLE  G+ R  +G  +   P++L  S + E+   +     + + + D G +
Sbjct: 278 REELNEIVEYLESNGVRRDWMGYVVGRCPELLSFS-MEEVKSRVDFFLKMGMNQNDFGTM 337

Query: 205 LQKYPELLGFKLEGTMSTSVAYLVSIGVNPRDIGPMVTQYPYFLGMRVGTMIKPLVEFIV 264
           +  YP+++GF     M   + YL   G++  ++G ++   P+ +G  +    KPLV++  
Sbjct: 338 VYDYPKIIGFFSFQVMEKKINYLKEFGLSTEEVGRLLAYKPHLMGCSIEERWKPLVKYFY 397

Query: 265 SLGLPKKIVARMLEKRAYILGYDLEETVKPNIDCLLSFGIRKELLPSVIAQYPQILGLPL 324
            LG+PK+ + R+L  +  +   DLE+T+ P +  L   GI  E + +++ ++P +L   L
Sbjct: 398 YLGIPKEGMKRILVVKPILYCIDLEKTIAPKVRFLQEMGIPNEAIGNMLVKFPSLLTNSL 457

Query: 325 KAKLSSQQFFFDLKLKIDPAGFAQVIEKMPQIVSLHQHVIIKP-AEFFLGRGIPSSDVAK 384
             K+     F   +  +      +VI   P ++       ++P   +++  GI    + +
Sbjct: 458 YKKIRPVVIFLLTRAGVTQKDIGKVIAMDPALLGCSIGTKLEPNMRYYISLGIRFYQLGE 517

Query: 385 MIIKCPQLVACRVELMKNSYYFFKSEMGRPIKELVNFPEYFTYSLESRIKPRYERLQSKG 444
           MI   P L+   V+ ++  Y + +  M RP+++L+ FP +F+YSLE RI PR+  +    
Sbjct: 518 MIADFPMLLRYNVDNLRPKYRYLRRTMIRPLQDLIEFPRFFSYSLERRIIPRHTIMVENR 577

Query: 445 IRCSLNWFLNCSDQRFEERLKGEF---------IETESLRPSFCMGGRLELPGNEIIVSD 504
           +   L + L C+D+ FE R++ +          +++E  +PS        +   EI  SD
Sbjct: 578 VNFKLRYMLACTDEEFERRVRDKVERRERFEAGLDSEDSQPS-----DENISDQEIAFSD 637

[Close]
BLAST of Bhi11G001312 vs. ExPASy Swiss-Prot
Match: Q9SZL6 (Transcription termination factor MTERF6, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MTERF6 PE=2 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 8.3e-27
Identity = 73/322 (22.67%), Postives = 159/322 (49.38%), Query Frame = 0 

Query: 147 NIIPVLGYLEKIGIPRPKLGEFIKNYPQVLHASVIVELAPVIKLLRGLDVEKEDIGYVLQ 206
           N   ++ +    G   P + + ++   Q+  A   V       L   + +++  + Y++ 
Sbjct: 5   NTSSIMWFFRDKGFDDPSIDKMLRKCKQLEKAQSDVASENWDYLSNIVGIQERKLPYIVS 64

Query: 207 KYPELLGFKLEGTMSTSVAYLVSIGVNPRDIGPMVTQYPYFLGMRVGTMIKPLVEFIVSL 266
           + P++L  +L+  +   V  L S+G NPR++   +T++P  L   V   + PL+ F  +L
Sbjct: 65  RCPKILTLRLDERLIPMVECLSSLGRNPREVASAITKFPPILSHSVEEKLCPLLAFFQAL 124

Query: 267 GLPKKIVARMLEKRAYILGYDLEETVKPNIDCLLSFGIRKE-LLPSVIAQYPQILGLPLK 326
           G+P+  + +M+     ++ Y ++  +   +  L S G+ ++ ++  V+ + P ++G  + 
Sbjct: 125 GVPETQLGKMILFNPRLISYSIDTKLTVIVSFLASLGLDQDGMIGKVLVKNPFLMGYSVD 184

Query: 327 AKLSSQQFFFDLKLKIDPAGFAQVIEKMPQIVSLHQHVIIKPAEFFLGR-GIPSSDVAKM 386
            +L     F    + +   G   V+   PQ++    + I+KP   +L   G   S +A M
Sbjct: 185 KRLRPTTEFLKSSVGLSEDGIKSVVMNFPQLLCRDVNKILKPNYDYLKECGFGDSQIATM 244

Query: 387 IIKCPQLVACRVELMKNSYY----FFKSEMGRPIKELVNFPEYFTYSLESRIKPRYERLQ 446
           +   PQ++   ++ +KNS      F    MGR + E+ ++PE+F + L+ +++ R++ ++
Sbjct: 245 VTGYPQIL---IKSVKNSLQPRIRFLVQVMGRGMDEVASYPEFFHHGLKKKVESRFKLVK 304

Query: 447 SKGIRCSLNWFLNCSDQRFEER 463
              I CSL   L+C+ ++F E+
Sbjct: 305 KNNIDCSLREMLDCNTKKFHEK 323

[Close]
BLAST of Bhi11G001312 vs. NCBI nr
Match: XP_038905918.1 (transcription termination factor MTERF4, chloroplastic [Benincasa hispida] >XP_038905920.1 transcription termination factor MTERF4, chloroplastic [Benincasa hispida] >XP_038905921.1 transcription termination factor MTERF4, chloroplastic [Benincasa hispida] >XP_038905922.1 transcription termination factor MTERF4, chloroplastic [Benincasa hispida] >XP_038905923.1 transcription termination factor MTERF4, chloroplastic [Benincasa hispida] >XP_038905924.1 transcription termination factor MTERF4, chloroplastic [Benincasa hispida])

HSP 1 Score: 1033.5 bits (2671), Expect = 6.2e-298
Identity = 513/513 (100.00%), Postives = 513/513 (100.00%), Query Frame = 0 

Query: 1   MRPRKSAKFSLVLGIAHQIYEKFGLFCPKITKSYLNPCAHQSHIQSFWRVFPLGVLYSTQ 60
           MRPRKSAKFSLVLGIAHQIYEKFGLFCPKITKSYLNPCAHQSHIQSFWRVFPLGVLYSTQ
Sbjct: 1   MRPRKSAKFSLVLGIAHQIYEKFGLFCPKITKSYLNPCAHQSHIQSFWRVFPLGVLYSTQ 60

Query: 61  SSKLPEYEMPTVTWGAVQGRKEKLVSRVIICDYLKSLGIVPDELEHLELPSTVEVMRERV 120
           SSKLPEYEMPTVTWGAVQGRKEKLVSRVIICDYLKSLGIVPDELEHLELPSTVEVMRERV
Sbjct: 61  SSKLPEYEMPTVTWGAVQGRKEKLVSRVIICDYLKSLGIVPDELEHLELPSTVEVMRERV 120

Query: 121 EFLQKLGLTIDDINEFPLMLGCSVRKNIIPVLGYLEKIGIPRPKLGEFIKNYPQVLHASV 180
           EFLQKLGLTIDDINEFPLMLGCSVRKNIIPVLGYLEKIGIPRPKLGEFIKNYPQVLHASV
Sbjct: 121 EFLQKLGLTIDDINEFPLMLGCSVRKNIIPVLGYLEKIGIPRPKLGEFIKNYPQVLHASV 180

Query: 181 IVELAPVIKLLRGLDVEKEDIGYVLQKYPELLGFKLEGTMSTSVAYLVSIGVNPRDIGPM 240
           IVELAPVIKLLRGLDVEKEDIGYVLQKYPELLGFKLEGTMSTSVAYLVSIGVNPRDIGPM
Sbjct: 181 IVELAPVIKLLRGLDVEKEDIGYVLQKYPELLGFKLEGTMSTSVAYLVSIGVNPRDIGPM 240

Query: 241 VTQYPYFLGMRVGTMIKPLVEFIVSLGLPKKIVARMLEKRAYILGYDLEETVKPNIDCLL 300
           VTQYPYFLGMRVGTMIKPLVEFIVSLGLPKKIVARMLEKRAYILGYDLEETVKPNIDCLL
Sbjct: 241 VTQYPYFLGMRVGTMIKPLVEFIVSLGLPKKIVARMLEKRAYILGYDLEETVKPNIDCLL 300

Query: 301 SFGIRKELLPSVIAQYPQILGLPLKAKLSSQQFFFDLKLKIDPAGFAQVIEKMPQIVSLH 360
           SFGIRKELLPSVIAQYPQILGLPLKAKLSSQQFFFDLKLKIDPAGFAQVIEKMPQIVSLH
Sbjct: 301 SFGIRKELLPSVIAQYPQILGLPLKAKLSSQQFFFDLKLKIDPAGFAQVIEKMPQIVSLH 360

Query: 361 QHVIIKPAEFFLGRGIPSSDVAKMIIKCPQLVACRVELMKNSYYFFKSEMGRPIKELVNF 420
           QHVIIKPAEFFLGRGIPSSDVAKMIIKCPQLVACRVELMKNSYYFFKSEMGRPIKELVNF
Sbjct: 361 QHVIIKPAEFFLGRGIPSSDVAKMIIKCPQLVACRVELMKNSYYFFKSEMGRPIKELVNF 420

Query: 421 PEYFTYSLESRIKPRYERLQSKGIRCSLNWFLNCSDQRFEERLKGEFIETESLRPSFCMG 480
           PEYFTYSLESRIKPRYERLQSKGIRCSLNWFLNCSDQRFEERLKGEFIETESLRPSFCMG
Sbjct: 421 PEYFTYSLESRIKPRYERLQSKGIRCSLNWFLNCSDQRFEERLKGEFIETESLRPSFCMG 480

Query: 481 GRLELPGNEIIVSDDEVEEDECDEKLHRRIVSL 514
           GRLELPGNEIIVSDDEVEEDECDEKLHRRIVSL
Sbjct: 481 GRLELPGNEIIVSDDEVEEDECDEKLHRRIVSL 513

[Close]
BLAST of Bhi11G001312 vs. NCBI nr
Match: XP_004141131.1 (transcription termination factor MTERF4, chloroplastic [Cucumis sativus] >KGN59741.1 hypothetical protein Csa_001560 [Cucumis sativus])

HSP 1 Score: 965.7 bits (2495), Expect = 1.6e-277
Identity = 479/513 (93.37%), Postives = 496/513 (96.69%), Query Frame = 0 

Query: 1   MRPRKSAKFSLVLGIAHQIYEKFGLFCPKITKSYLNPCAHQSHIQSFWRVFPLGVLYSTQ 60
           +RPRKSA  SLV GI+ Q++EKFGLFCPKI KS+LNPCAHQSHIQSFWRVFPLGVLYSTQ
Sbjct: 7   LRPRKSAICSLVFGISPQVFEKFGLFCPKIVKSFLNPCAHQSHIQSFWRVFPLGVLYSTQ 66

Query: 61  SSKLPEYEMPTVTWGAVQGRKEKLVSRVIICDYLKSLGIVPDELEHLELPSTVEVMRERV 120
           SSKLPEYEMPTVTWGAVQGRKEKLVSRVIICDYLKSLGIVPDELEHLELPSTVEVMRERV
Sbjct: 67  SSKLPEYEMPTVTWGAVQGRKEKLVSRVIICDYLKSLGIVPDELEHLELPSTVEVMRERV 126

Query: 121 EFLQKLGLTIDDINEFPLMLGCSVRKNIIPVLGYLEKIGIPRPKLGEFIKNYPQVLHASV 180
           EFLQKLGLTIDDINEFPL+LGCSVRKN+IPVLGYLEKIGIPRPKLGEFIKNYPQVLHASV
Sbjct: 127 EFLQKLGLTIDDINEFPLILGCSVRKNMIPVLGYLEKIGIPRPKLGEFIKNYPQVLHASV 186

Query: 181 IVELAPVIKLLRGLDVEKEDIGYVLQKYPELLGFKLEGTMSTSVAYLVSIGVNPRDIGPM 240
           IVELAPVIKLLRGLDVEKEDIGYVLQKYPELLGFKLEGTMSTSVAYLVSIGVNPRDIGPM
Sbjct: 187 IVELAPVIKLLRGLDVEKEDIGYVLQKYPELLGFKLEGTMSTSVAYLVSIGVNPRDIGPM 246

Query: 241 VTQYPYFLGMRVGTMIKPLVEFIVSLGLPKKIVARMLEKRAYILGYDLEETVKPNIDCLL 300
           VTQYP+FLGMRVGTMIKPLVEFIVSLGLPKKIVARMLEKRAYILGYDL ETVKPNIDCLL
Sbjct: 247 VTQYPFFLGMRVGTMIKPLVEFIVSLGLPKKIVARMLEKRAYILGYDLGETVKPNIDCLL 306

Query: 301 SFGIRKELLPSVIAQYPQILGLPLKAKLSSQQFFFDLKLKIDPAGFAQVIEKMPQIVSLH 360
           SFGIRKELLPSVIAQYP ILGLPLKAK+SSQQFFFDLKLKIDPAGFAQVIEKMPQ+VSLH
Sbjct: 307 SFGIRKELLPSVIAQYPLILGLPLKAKMSSQQFFFDLKLKIDPAGFAQVIEKMPQMVSLH 366

Query: 361 QHVIIKPAEFFLGRGIPSSDVAKMIIKCPQLVACRVELMKNSYYFFKSEMGRPIKELVNF 420
           QHVIIKPAEF L RGI SSDVAKMI++CPQL+ACRV LMKNSYYFFKS+MGRPIKELV+F
Sbjct: 367 QHVIIKPAEFLLERGIASSDVAKMIVQCPQLLACRVPLMKNSYYFFKSDMGRPIKELVDF 426

Query: 421 PEYFTYSLESRIKPRYERLQSKGIRCSLNWFLNCSDQRFEERLKGEFIETESLRPSFCMG 480
           PEYFTYSLESRIKPRY+RLQSKGI CSLNWFLNCSDQRFEERLKGEFIETESLRPSFCMG
Sbjct: 427 PEYFTYSLESRIKPRYQRLQSKGISCSLNWFLNCSDQRFEERLKGEFIETESLRPSFCMG 486

Query: 481 GRLELPGNEIIVSDDEVEEDECDEKLHRRIVSL 514
           GRLELP NE IVSDD   EDECDE+LHRRIVSL
Sbjct: 487 GRLELPSNETIVSDD---EDECDEQLHRRIVSL 516

[Close]
BLAST of Bhi11G001312 vs. NCBI nr
Match: XP_008464974.1 (PREDICTED: transcription termination factor MTERF4, chloroplastic [Cucumis melo] >KAA0057178.1 transcription termination factor MTERF4 [Cucumis melo var. makuwa] >TYK18782.1 transcription termination factor MTERF4 [Cucumis melo var. makuwa])

HSP 1 Score: 964.5 bits (2492), Expect = 3.6e-277
Identity = 473/513 (92.20%), Postives = 495/513 (96.49%), Query Frame = 0 

Query: 1   MRPRKSAKFSLVLGIAHQIYEKFGLFCPKITKSYLNPCAHQSHIQSFWRVFPLGVLYSTQ 60
           +RPRKSA FS V G++ Q+++KFGLFCPKI KS+L+PCAH S+ QSFWRVF LGVLYSTQ
Sbjct: 7   LRPRKSAIFSFVFGVSPQVFQKFGLFCPKIAKSFLSPCAHLSNNQSFWRVFSLGVLYSTQ 66

Query: 61  SSKLPEYEMPTVTWGAVQGRKEKLVSRVIICDYLKSLGIVPDELEHLELPSTVEVMRERV 120
           SSKLPEYE+PTVTWGAVQGRKEKLVSRVIICDYLKSLGIVPDELEHLELPS VEVMRERV
Sbjct: 67  SSKLPEYELPTVTWGAVQGRKEKLVSRVIICDYLKSLGIVPDELEHLELPSNVEVMRERV 126

Query: 121 EFLQKLGLTIDDINEFPLMLGCSVRKNIIPVLGYLEKIGIPRPKLGEFIKNYPQVLHASV 180
           EFLQKLGLTIDDINEFPLMLGCSVRKN+IPVLGYLEKIGIPRPKLGEFIKNYPQVLHASV
Sbjct: 127 EFLQKLGLTIDDINEFPLMLGCSVRKNMIPVLGYLEKIGIPRPKLGEFIKNYPQVLHASV 186

Query: 181 IVELAPVIKLLRGLDVEKEDIGYVLQKYPELLGFKLEGTMSTSVAYLVSIGVNPRDIGPM 240
           IVELAPVIKLLRGLDVEK+DIGYVLQKYPELLGFKLEGTMSTSVAYLVSIGVNPRDIGPM
Sbjct: 187 IVELAPVIKLLRGLDVEKDDIGYVLQKYPELLGFKLEGTMSTSVAYLVSIGVNPRDIGPM 246

Query: 241 VTQYPYFLGMRVGTMIKPLVEFIVSLGLPKKIVARMLEKRAYILGYDLEETVKPNIDCLL 300
           VTQYP+FLGMRVGTMIKPLVEFIVSLGLPKKIVARMLEKRAYILGYDLEETVKPNIDCLL
Sbjct: 247 VTQYPFFLGMRVGTMIKPLVEFIVSLGLPKKIVARMLEKRAYILGYDLEETVKPNIDCLL 306

Query: 301 SFGIRKELLPSVIAQYPQILGLPLKAKLSSQQFFFDLKLKIDPAGFAQVIEKMPQIVSLH 360
           SFG RKELLPS+IAQYP ILGLPLKAK+SSQQFFFDLKLK+DPAGFAQVIEKMPQIVSLH
Sbjct: 307 SFGTRKELLPSIIAQYPVILGLPLKAKMSSQQFFFDLKLKVDPAGFAQVIEKMPQIVSLH 366

Query: 361 QHVIIKPAEFFLGRGIPSSDVAKMIIKCPQLVACRVELMKNSYYFFKSEMGRPIKELVNF 420
           QHVI KPAEF L RGIPSSDVAKMI+KCPQL+ACRV LMKNSYYFFKS+MGRPIKELV+F
Sbjct: 367 QHVITKPAEFLLERGIPSSDVAKMIVKCPQLLACRVPLMKNSYYFFKSDMGRPIKELVDF 426

Query: 421 PEYFTYSLESRIKPRYERLQSKGIRCSLNWFLNCSDQRFEERLKGEFIETESLRPSFCMG 480
           PEYFTYSLESRIKPRY+RLQSKGI CSLNWFLNCSDQRFEERLKGEFIETESLRPSFCMG
Sbjct: 427 PEYFTYSLESRIKPRYQRLQSKGISCSLNWFLNCSDQRFEERLKGEFIETESLRPSFCMG 486

Query: 481 GRLELPGNEIIVSDDEVEEDECDEKLHRRIVSL 514
           GRLELP NE IVSDDEVEEDECDE+LHRRIVSL
Sbjct: 487 GRLELPSNETIVSDDEVEEDECDEQLHRRIVSL 519

[Close]
BLAST of Bhi11G001312 vs. NCBI nr
Match: XP_022980596.1 (transcription termination factor MTERF4, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 960.3 bits (2481), Expect = 6.7e-276
Identity = 477/514 (92.80%), Postives = 496/514 (96.50%), Query Frame = 0 

Query: 1   MRPRKSAKFSLVLGIAHQIYEKFGLFCPKITKSYLNPCAHQSHIQSFWRVFPLGVLYSTQ 60
           +RPRKSAKFSLVLGI+H IYE  GLFCPKITKS+LNPCAHQS IQSF RV PLGVLYSTQ
Sbjct: 7   LRPRKSAKFSLVLGISHHIYENLGLFCPKITKSFLNPCAHQSLIQSFCRVLPLGVLYSTQ 66

Query: 61  SSKLPEYEMPTVTWGAVQGRKEKLVSRVIICDYLKSLGIVPDELEHLELPSTVEVMRERV 120
           SSKLPEYEMPTVTWGAVQGRKEKLVSRVIICDYLKSLGIVPDELEHLELPSTVEVMRERV
Sbjct: 67  SSKLPEYEMPTVTWGAVQGRKEKLVSRVIICDYLKSLGIVPDELEHLELPSTVEVMRERV 126

Query: 121 EFLQKLGLTIDDINEFPLMLGCSVRKNIIPVLGYLEKIGIPRPKLGEFIKNYPQVLHASV 180
           EFLQKLGLTIDDINEFPLMLGCSVRKN+IPVLGYLEKIGIPRPKLGEFIKNYPQVLHASV
Sbjct: 127 EFLQKLGLTIDDINEFPLMLGCSVRKNMIPVLGYLEKIGIPRPKLGEFIKNYPQVLHASV 186

Query: 181 IVELAPVIKLLRGLDVEKEDIGYVLQKYPELLGFKLEGTMSTSVAYLVSIGVNPRDIGPM 240
           IVELAPV+KLLRGLDVEK+DIGYVLQK+PELLG+KLEGTMSTSVAYLVSIGVNPRDIGPM
Sbjct: 187 IVELAPVVKLLRGLDVEKQDIGYVLQKHPELLGYKLEGTMSTSVAYLVSIGVNPRDIGPM 246

Query: 241 VTQYPYFLGMRVGTMIKPLVEFIVSLGLPKKIVARMLEKRAYILGYDLEETVKPNIDCLL 300
           VTQYPYFL MRVGTMIKPLV+FIVSLGLPKKIVARMLEKRAYILGYDLEETV+PNIDCLL
Sbjct: 247 VTQYPYFLAMRVGTMIKPLVDFIVSLGLPKKIVARMLEKRAYILGYDLEETVRPNIDCLL 306

Query: 301 SFGIRKELLPSVIAQYPQILGLPLKAKLSSQQFFFDLKLKIDPAGFAQVIEKMPQIVSLH 360
           SFGIRKELLPSVIA YPQILGLPLKAKLS+QQFFFDLKLKIDPAGFAQVIEKMPQI+SLH
Sbjct: 307 SFGIRKELLPSVIAHYPQILGLPLKAKLSAQQFFFDLKLKIDPAGFAQVIEKMPQILSLH 366

Query: 361 QHVIIKPAEFFLGRGIPSSDVAKMIIKCPQLVACRVELMKNSYYFFKSEMGRPIKELVNF 420
           QHVI+KP EF LGRGIPSSDVAKM++KCPQLVACRVELMKNSYYFFKSEMGRPIKELV F
Sbjct: 367 QHVIMKPVEFLLGRGIPSSDVAKMVVKCPQLVACRVELMKNSYYFFKSEMGRPIKELVEF 426

Query: 421 PEYFTYSLESRIKPRYERLQSKGIRCSLNWFLNCSDQRFEERLKGEFIETESLRPSFCMG 480
           PEYFTYSLESRIKPRYER+Q KGIRCSLNWFLN S+QRFEERLKG+FIETESL PSFCMG
Sbjct: 427 PEYFTYSLESRIKPRYERVQRKGIRCSLNWFLNVSEQRFEERLKGDFIETESLGPSFCMG 486

Query: 481 GRLELPGNEIIVSDDEVEEDECDEK-LHRRIVSL 514
           GRLELPGNE IVSDD  EEDECDE+ LHRRIVSL
Sbjct: 487 GRLELPGNETIVSDD--EEDECDEQFLHRRIVSL 518

[Close]
BLAST of Bhi11G001312 vs. NCBI nr
Match: XP_022930565.1 (transcription termination factor MTERF4, chloroplastic [Cucurbita moschata])

HSP 1 Score: 952.6 bits (2461), Expect = 1.4e-273
Identity = 474/514 (92.22%), Postives = 493/514 (95.91%), Query Frame = 0 

Query: 1   MRPRKSAKFSLVLGIAHQIYEKFGLFCPKITKSYLNPCAHQSHIQSFWRVFPLGVLYSTQ 60
           +RPRKSAKFSLVLGI+H IYE  GLFCPKITKS+LNPCAHQS IQSF RV PL VLYSTQ
Sbjct: 7   LRPRKSAKFSLVLGISHHIYENLGLFCPKITKSFLNPCAHQSLIQSFCRVLPLSVLYSTQ 66

Query: 61  SSKLPEYEMPTVTWGAVQGRKEKLVSRVIICDYLKSLGIVPDELEHLELPSTVEVMRERV 120
           SSKL EYEMPTVTWGAVQGRKEKLVSRVIICDYLKSLGIVPDELEHLELPSTVEVMRERV
Sbjct: 67  SSKLHEYEMPTVTWGAVQGRKEKLVSRVIICDYLKSLGIVPDELEHLELPSTVEVMRERV 126

Query: 121 EFLQKLGLTIDDINEFPLMLGCSVRKNIIPVLGYLEKIGIPRPKLGEFIKNYPQVLHASV 180
           EFLQKLGLTIDDINEFPLMLGCSVRKN+IPVLGYLEKIGIPRPKLGEFIKNYPQVLHASV
Sbjct: 127 EFLQKLGLTIDDINEFPLMLGCSVRKNMIPVLGYLEKIGIPRPKLGEFIKNYPQVLHASV 186

Query: 181 IVELAPVIKLLRGLDVEKEDIGYVLQKYPELLGFKLEGTMSTSVAYLVSIGVNPRDIGPM 240
           IVELAPV+KLLRGLDVEKEDIGYVLQK+PELLG+KLEGTMSTSVAYLVSIGVNPRDIGPM
Sbjct: 187 IVELAPVVKLLRGLDVEKEDIGYVLQKHPELLGYKLEGTMSTSVAYLVSIGVNPRDIGPM 246

Query: 241 VTQYPYFLGMRVGTMIKPLVEFIVSLGLPKKIVARMLEKRAYILGYDLEETVKPNIDCLL 300
           VTQYPYFL MRVGTMIKPLV+FIVSLGLPKKIVARMLEKRAYILGYDLEETV+PNIDCLL
Sbjct: 247 VTQYPYFLAMRVGTMIKPLVDFIVSLGLPKKIVARMLEKRAYILGYDLEETVRPNIDCLL 306

Query: 301 SFGIRKELLPSVIAQYPQILGLPLKAKLSSQQFFFDLKLKIDPAGFAQVIEKMPQIVSLH 360
           SFGIRKELLPSVIA YPQILGLPLKAKLS+QQFFFDLKLKIDPAGFAQVIEKMPQI+ LH
Sbjct: 307 SFGIRKELLPSVIAHYPQILGLPLKAKLSAQQFFFDLKLKIDPAGFAQVIEKMPQILCLH 366

Query: 361 QHVIIKPAEFFLGRGIPSSDVAKMIIKCPQLVACRVELMKNSYYFFKSEMGRPIKELVNF 420
           QHVI+KP EF LGRGIPSSDVAKM++KCPQLVACRVELMKNSYYFFKSEMGRPIKELV F
Sbjct: 367 QHVIMKPVEFLLGRGIPSSDVAKMVVKCPQLVACRVELMKNSYYFFKSEMGRPIKELVEF 426

Query: 421 PEYFTYSLESRIKPRYERLQSKGIRCSLNWFLNCSDQRFEERLKGEFIETESLRPSFCMG 480
           PEYFTYSLESRIKPRYER+Q KGIRCSLNWFLN S+QRFEERLKG+FIETESL PSFCMG
Sbjct: 427 PEYFTYSLESRIKPRYERVQRKGIRCSLNWFLNVSEQRFEERLKGDFIETESLGPSFCMG 486

Query: 481 GRLELPGNEIIVSDDEVEEDECDEK-LHRRIVSL 514
           GRLE+PGNE IVSDD  EEDECDE+ LHRRIVSL
Sbjct: 487 GRLEVPGNETIVSDD--EEDECDEQFLHRRIVSL 518

[Close]
BLAST of Bhi11G001312 vs. ExPASy TrEMBL
Match: A0A0A0LI61 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G842630 PE=3 SV=1)

HSP 1 Score: 965.7 bits (2495), Expect = 7.7e-278
Identity = 479/513 (93.37%), Postives = 496/513 (96.69%), Query Frame = 0 

Query: 1   MRPRKSAKFSLVLGIAHQIYEKFGLFCPKITKSYLNPCAHQSHIQSFWRVFPLGVLYSTQ 60
           +RPRKSA  SLV GI+ Q++EKFGLFCPKI KS+LNPCAHQSHIQSFWRVFPLGVLYSTQ
Sbjct: 7   LRPRKSAICSLVFGISPQVFEKFGLFCPKIVKSFLNPCAHQSHIQSFWRVFPLGVLYSTQ 66

Query: 61  SSKLPEYEMPTVTWGAVQGRKEKLVSRVIICDYLKSLGIVPDELEHLELPSTVEVMRERV 120
           SSKLPEYEMPTVTWGAVQGRKEKLVSRVIICDYLKSLGIVPDELEHLELPSTVEVMRERV
Sbjct: 67  SSKLPEYEMPTVTWGAVQGRKEKLVSRVIICDYLKSLGIVPDELEHLELPSTVEVMRERV 126

Query: 121 EFLQKLGLTIDDINEFPLMLGCSVRKNIIPVLGYLEKIGIPRPKLGEFIKNYPQVLHASV 180
           EFLQKLGLTIDDINEFPL+LGCSVRKN+IPVLGYLEKIGIPRPKLGEFIKNYPQVLHASV
Sbjct: 127 EFLQKLGLTIDDINEFPLILGCSVRKNMIPVLGYLEKIGIPRPKLGEFIKNYPQVLHASV 186

Query: 181 IVELAPVIKLLRGLDVEKEDIGYVLQKYPELLGFKLEGTMSTSVAYLVSIGVNPRDIGPM 240
           IVELAPVIKLLRGLDVEKEDIGYVLQKYPELLGFKLEGTMSTSVAYLVSIGVNPRDIGPM
Sbjct: 187 IVELAPVIKLLRGLDVEKEDIGYVLQKYPELLGFKLEGTMSTSVAYLVSIGVNPRDIGPM 246

Query: 241 VTQYPYFLGMRVGTMIKPLVEFIVSLGLPKKIVARMLEKRAYILGYDLEETVKPNIDCLL 300
           VTQYP+FLGMRVGTMIKPLVEFIVSLGLPKKIVARMLEKRAYILGYDL ETVKPNIDCLL
Sbjct: 247 VTQYPFFLGMRVGTMIKPLVEFIVSLGLPKKIVARMLEKRAYILGYDLGETVKPNIDCLL 306

Query: 301 SFGIRKELLPSVIAQYPQILGLPLKAKLSSQQFFFDLKLKIDPAGFAQVIEKMPQIVSLH 360
           SFGIRKELLPSVIAQYP ILGLPLKAK+SSQQFFFDLKLKIDPAGFAQVIEKMPQ+VSLH
Sbjct: 307 SFGIRKELLPSVIAQYPLILGLPLKAKMSSQQFFFDLKLKIDPAGFAQVIEKMPQMVSLH 366

Query: 361 QHVIIKPAEFFLGRGIPSSDVAKMIIKCPQLVACRVELMKNSYYFFKSEMGRPIKELVNF 420
           QHVIIKPAEF L RGI SSDVAKMI++CPQL+ACRV LMKNSYYFFKS+MGRPIKELV+F
Sbjct: 367 QHVIIKPAEFLLERGIASSDVAKMIVQCPQLLACRVPLMKNSYYFFKSDMGRPIKELVDF 426

Query: 421 PEYFTYSLESRIKPRYERLQSKGIRCSLNWFLNCSDQRFEERLKGEFIETESLRPSFCMG 480
           PEYFTYSLESRIKPRY+RLQSKGI CSLNWFLNCSDQRFEERLKGEFIETESLRPSFCMG
Sbjct: 427 PEYFTYSLESRIKPRYQRLQSKGISCSLNWFLNCSDQRFEERLKGEFIETESLRPSFCMG 486

Query: 481 GRLELPGNEIIVSDDEVEEDECDEKLHRRIVSL 514
           GRLELP NE IVSDD   EDECDE+LHRRIVSL
Sbjct: 487 GRLELPSNETIVSDD---EDECDEQLHRRIVSL 516

[Close]
BLAST of Bhi11G001312 vs. ExPASy TrEMBL
Match: A0A5D3D5I6 (Transcription termination factor MTERF4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold257G00900 PE=3 SV=1)

HSP 1 Score: 964.5 bits (2492), Expect = 1.7e-277
Identity = 473/513 (92.20%), Postives = 495/513 (96.49%), Query Frame = 0 

Query: 1   MRPRKSAKFSLVLGIAHQIYEKFGLFCPKITKSYLNPCAHQSHIQSFWRVFPLGVLYSTQ 60
           +RPRKSA FS V G++ Q+++KFGLFCPKI KS+L+PCAH S+ QSFWRVF LGVLYSTQ
Sbjct: 7   LRPRKSAIFSFVFGVSPQVFQKFGLFCPKIAKSFLSPCAHLSNNQSFWRVFSLGVLYSTQ 66

Query: 61  SSKLPEYEMPTVTWGAVQGRKEKLVSRVIICDYLKSLGIVPDELEHLELPSTVEVMRERV 120
           SSKLPEYE+PTVTWGAVQGRKEKLVSRVIICDYLKSLGIVPDELEHLELPS VEVMRERV
Sbjct: 67  SSKLPEYELPTVTWGAVQGRKEKLVSRVIICDYLKSLGIVPDELEHLELPSNVEVMRERV 126

Query: 121 EFLQKLGLTIDDINEFPLMLGCSVRKNIIPVLGYLEKIGIPRPKLGEFIKNYPQVLHASV 180
           EFLQKLGLTIDDINEFPLMLGCSVRKN+IPVLGYLEKIGIPRPKLGEFIKNYPQVLHASV
Sbjct: 127 EFLQKLGLTIDDINEFPLMLGCSVRKNMIPVLGYLEKIGIPRPKLGEFIKNYPQVLHASV 186

Query: 181 IVELAPVIKLLRGLDVEKEDIGYVLQKYPELLGFKLEGTMSTSVAYLVSIGVNPRDIGPM 240
           IVELAPVIKLLRGLDVEK+DIGYVLQKYPELLGFKLEGTMSTSVAYLVSIGVNPRDIGPM
Sbjct: 187 IVELAPVIKLLRGLDVEKDDIGYVLQKYPELLGFKLEGTMSTSVAYLVSIGVNPRDIGPM 246

Query: 241 VTQYPYFLGMRVGTMIKPLVEFIVSLGLPKKIVARMLEKRAYILGYDLEETVKPNIDCLL 300
           VTQYP+FLGMRVGTMIKPLVEFIVSLGLPKKIVARMLEKRAYILGYDLEETVKPNIDCLL
Sbjct: 247 VTQYPFFLGMRVGTMIKPLVEFIVSLGLPKKIVARMLEKRAYILGYDLEETVKPNIDCLL 306

Query: 301 SFGIRKELLPSVIAQYPQILGLPLKAKLSSQQFFFDLKLKIDPAGFAQVIEKMPQIVSLH 360
           SFG RKELLPS+IAQYP ILGLPLKAK+SSQQFFFDLKLK+DPAGFAQVIEKMPQIVSLH
Sbjct: 307 SFGTRKELLPSIIAQYPVILGLPLKAKMSSQQFFFDLKLKVDPAGFAQVIEKMPQIVSLH 366

Query: 361 QHVIIKPAEFFLGRGIPSSDVAKMIIKCPQLVACRVELMKNSYYFFKSEMGRPIKELVNF 420
           QHVI KPAEF L RGIPSSDVAKMI+KCPQL+ACRV LMKNSYYFFKS+MGRPIKELV+F
Sbjct: 367 QHVITKPAEFLLERGIPSSDVAKMIVKCPQLLACRVPLMKNSYYFFKSDMGRPIKELVDF 426

Query: 421 PEYFTYSLESRIKPRYERLQSKGIRCSLNWFLNCSDQRFEERLKGEFIETESLRPSFCMG 480
           PEYFTYSLESRIKPRY+RLQSKGI CSLNWFLNCSDQRFEERLKGEFIETESLRPSFCMG
Sbjct: 427 PEYFTYSLESRIKPRYQRLQSKGISCSLNWFLNCSDQRFEERLKGEFIETESLRPSFCMG 486

Query: 481 GRLELPGNEIIVSDDEVEEDECDEKLHRRIVSL 514
           GRLELP NE IVSDDEVEEDECDE+LHRRIVSL
Sbjct: 487 GRLELPSNETIVSDDEVEEDECDEQLHRRIVSL 519

[Close]
BLAST of Bhi11G001312 vs. ExPASy TrEMBL
Match: A0A1S3CN78 (transcription termination factor MTERF4, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103502714 PE=3 SV=1)

HSP 1 Score: 964.5 bits (2492), Expect = 1.7e-277
Identity = 473/513 (92.20%), Postives = 495/513 (96.49%), Query Frame = 0 

Query: 1   MRPRKSAKFSLVLGIAHQIYEKFGLFCPKITKSYLNPCAHQSHIQSFWRVFPLGVLYSTQ 60
           +RPRKSA FS V G++ Q+++KFGLFCPKI KS+L+PCAH S+ QSFWRVF LGVLYSTQ
Sbjct: 7   LRPRKSAIFSFVFGVSPQVFQKFGLFCPKIAKSFLSPCAHLSNNQSFWRVFSLGVLYSTQ 66

Query: 61  SSKLPEYEMPTVTWGAVQGRKEKLVSRVIICDYLKSLGIVPDELEHLELPSTVEVMRERV 120
           SSKLPEYE+PTVTWGAVQGRKEKLVSRVIICDYLKSLGIVPDELEHLELPS VEVMRERV
Sbjct: 67  SSKLPEYELPTVTWGAVQGRKEKLVSRVIICDYLKSLGIVPDELEHLELPSNVEVMRERV 126

Query: 121 EFLQKLGLTIDDINEFPLMLGCSVRKNIIPVLGYLEKIGIPRPKLGEFIKNYPQVLHASV 180
           EFLQKLGLTIDDINEFPLMLGCSVRKN+IPVLGYLEKIGIPRPKLGEFIKNYPQVLHASV
Sbjct: 127 EFLQKLGLTIDDINEFPLMLGCSVRKNMIPVLGYLEKIGIPRPKLGEFIKNYPQVLHASV 186

Query: 181 IVELAPVIKLLRGLDVEKEDIGYVLQKYPELLGFKLEGTMSTSVAYLVSIGVNPRDIGPM 240
           IVELAPVIKLLRGLDVEK+DIGYVLQKYPELLGFKLEGTMSTSVAYLVSIGVNPRDIGPM
Sbjct: 187 IVELAPVIKLLRGLDVEKDDIGYVLQKYPELLGFKLEGTMSTSVAYLVSIGVNPRDIGPM 246

Query: 241 VTQYPYFLGMRVGTMIKPLVEFIVSLGLPKKIVARMLEKRAYILGYDLEETVKPNIDCLL 300
           VTQYP+FLGMRVGTMIKPLVEFIVSLGLPKKIVARMLEKRAYILGYDLEETVKPNIDCLL
Sbjct: 247 VTQYPFFLGMRVGTMIKPLVEFIVSLGLPKKIVARMLEKRAYILGYDLEETVKPNIDCLL 306

Query: 301 SFGIRKELLPSVIAQYPQILGLPLKAKLSSQQFFFDLKLKIDPAGFAQVIEKMPQIVSLH 360
           SFG RKELLPS+IAQYP ILGLPLKAK+SSQQFFFDLKLK+DPAGFAQVIEKMPQIVSLH
Sbjct: 307 SFGTRKELLPSIIAQYPVILGLPLKAKMSSQQFFFDLKLKVDPAGFAQVIEKMPQIVSLH 366

Query: 361 QHVIIKPAEFFLGRGIPSSDVAKMIIKCPQLVACRVELMKNSYYFFKSEMGRPIKELVNF 420
           QHVI KPAEF L RGIPSSDVAKMI+KCPQL+ACRV LMKNSYYFFKS+MGRPIKELV+F
Sbjct: 367 QHVITKPAEFLLERGIPSSDVAKMIVKCPQLLACRVPLMKNSYYFFKSDMGRPIKELVDF 426

Query: 421 PEYFTYSLESRIKPRYERLQSKGIRCSLNWFLNCSDQRFEERLKGEFIETESLRPSFCMG 480
           PEYFTYSLESRIKPRY+RLQSKGI CSLNWFLNCSDQRFEERLKGEFIETESLRPSFCMG
Sbjct: 427 PEYFTYSLESRIKPRYQRLQSKGISCSLNWFLNCSDQRFEERLKGEFIETESLRPSFCMG 486

Query: 481 GRLELPGNEIIVSDDEVEEDECDEKLHRRIVSL 514
           GRLELP NE IVSDDEVEEDECDE+LHRRIVSL
Sbjct: 487 GRLELPSNETIVSDDEVEEDECDEQLHRRIVSL 519

[Close]
BLAST of Bhi11G001312 vs. ExPASy TrEMBL
Match: A0A6J1IU10 (transcription termination factor MTERF4, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111479919 PE=3 SV=1)

HSP 1 Score: 960.3 bits (2481), Expect = 3.2e-276
Identity = 477/514 (92.80%), Postives = 496/514 (96.50%), Query Frame = 0 

Query: 1   MRPRKSAKFSLVLGIAHQIYEKFGLFCPKITKSYLNPCAHQSHIQSFWRVFPLGVLYSTQ 60
           +RPRKSAKFSLVLGI+H IYE  GLFCPKITKS+LNPCAHQS IQSF RV PLGVLYSTQ
Sbjct: 7   LRPRKSAKFSLVLGISHHIYENLGLFCPKITKSFLNPCAHQSLIQSFCRVLPLGVLYSTQ 66

Query: 61  SSKLPEYEMPTVTWGAVQGRKEKLVSRVIICDYLKSLGIVPDELEHLELPSTVEVMRERV 120
           SSKLPEYEMPTVTWGAVQGRKEKLVSRVIICDYLKSLGIVPDELEHLELPSTVEVMRERV
Sbjct: 67  SSKLPEYEMPTVTWGAVQGRKEKLVSRVIICDYLKSLGIVPDELEHLELPSTVEVMRERV 126

Query: 121 EFLQKLGLTIDDINEFPLMLGCSVRKNIIPVLGYLEKIGIPRPKLGEFIKNYPQVLHASV 180
           EFLQKLGLTIDDINEFPLMLGCSVRKN+IPVLGYLEKIGIPRPKLGEFIKNYPQVLHASV
Sbjct: 127 EFLQKLGLTIDDINEFPLMLGCSVRKNMIPVLGYLEKIGIPRPKLGEFIKNYPQVLHASV 186

Query: 181 IVELAPVIKLLRGLDVEKEDIGYVLQKYPELLGFKLEGTMSTSVAYLVSIGVNPRDIGPM 240
           IVELAPV+KLLRGLDVEK+DIGYVLQK+PELLG+KLEGTMSTSVAYLVSIGVNPRDIGPM
Sbjct: 187 IVELAPVVKLLRGLDVEKQDIGYVLQKHPELLGYKLEGTMSTSVAYLVSIGVNPRDIGPM 246

Query: 241 VTQYPYFLGMRVGTMIKPLVEFIVSLGLPKKIVARMLEKRAYILGYDLEETVKPNIDCLL 300
           VTQYPYFL MRVGTMIKPLV+FIVSLGLPKKIVARMLEKRAYILGYDLEETV+PNIDCLL
Sbjct: 247 VTQYPYFLAMRVGTMIKPLVDFIVSLGLPKKIVARMLEKRAYILGYDLEETVRPNIDCLL 306

Query: 301 SFGIRKELLPSVIAQYPQILGLPLKAKLSSQQFFFDLKLKIDPAGFAQVIEKMPQIVSLH 360
           SFGIRKELLPSVIA YPQILGLPLKAKLS+QQFFFDLKLKIDPAGFAQVIEKMPQI+SLH
Sbjct: 307 SFGIRKELLPSVIAHYPQILGLPLKAKLSAQQFFFDLKLKIDPAGFAQVIEKMPQILSLH 366

Query: 361 QHVIIKPAEFFLGRGIPSSDVAKMIIKCPQLVACRVELMKNSYYFFKSEMGRPIKELVNF 420
           QHVI+KP EF LGRGIPSSDVAKM++KCPQLVACRVELMKNSYYFFKSEMGRPIKELV F
Sbjct: 367 QHVIMKPVEFLLGRGIPSSDVAKMVVKCPQLVACRVELMKNSYYFFKSEMGRPIKELVEF 426

Query: 421 PEYFTYSLESRIKPRYERLQSKGIRCSLNWFLNCSDQRFEERLKGEFIETESLRPSFCMG 480
           PEYFTYSLESRIKPRYER+Q KGIRCSLNWFLN S+QRFEERLKG+FIETESL PSFCMG
Sbjct: 427 PEYFTYSLESRIKPRYERVQRKGIRCSLNWFLNVSEQRFEERLKGDFIETESLGPSFCMG 486

Query: 481 GRLELPGNEIIVSDDEVEEDECDEK-LHRRIVSL 514
           GRLELPGNE IVSDD  EEDECDE+ LHRRIVSL
Sbjct: 487 GRLELPGNETIVSDD--EEDECDEQFLHRRIVSL 518

[Close]
BLAST of Bhi11G001312 vs. ExPASy TrEMBL
Match: A0A6J1EX70 (transcription termination factor MTERF4, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111436975 PE=3 SV=1)

HSP 1 Score: 952.6 bits (2461), Expect = 6.8e-274
Identity = 474/514 (92.22%), Postives = 493/514 (95.91%), Query Frame = 0 

Query: 1   MRPRKSAKFSLVLGIAHQIYEKFGLFCPKITKSYLNPCAHQSHIQSFWRVFPLGVLYSTQ 60
           +RPRKSAKFSLVLGI+H IYE  GLFCPKITKS+LNPCAHQS IQSF RV PL VLYSTQ
Sbjct: 7   LRPRKSAKFSLVLGISHHIYENLGLFCPKITKSFLNPCAHQSLIQSFCRVLPLSVLYSTQ 66

Query: 61  SSKLPEYEMPTVTWGAVQGRKEKLVSRVIICDYLKSLGIVPDELEHLELPSTVEVMRERV 120
           SSKL EYEMPTVTWGAVQGRKEKLVSRVIICDYLKSLGIVPDELEHLELPSTVEVMRERV
Sbjct: 67  SSKLHEYEMPTVTWGAVQGRKEKLVSRVIICDYLKSLGIVPDELEHLELPSTVEVMRERV 126

Query: 121 EFLQKLGLTIDDINEFPLMLGCSVRKNIIPVLGYLEKIGIPRPKLGEFIKNYPQVLHASV 180
           EFLQKLGLTIDDINEFPLMLGCSVRKN+IPVLGYLEKIGIPRPKLGEFIKNYPQVLHASV
Sbjct: 127 EFLQKLGLTIDDINEFPLMLGCSVRKNMIPVLGYLEKIGIPRPKLGEFIKNYPQVLHASV 186

Query: 181 IVELAPVIKLLRGLDVEKEDIGYVLQKYPELLGFKLEGTMSTSVAYLVSIGVNPRDIGPM 240
           IVELAPV+KLLRGLDVEKEDIGYVLQK+PELLG+KLEGTMSTSVAYLVSIGVNPRDIGPM
Sbjct: 187 IVELAPVVKLLRGLDVEKEDIGYVLQKHPELLGYKLEGTMSTSVAYLVSIGVNPRDIGPM 246

Query: 241 VTQYPYFLGMRVGTMIKPLVEFIVSLGLPKKIVARMLEKRAYILGYDLEETVKPNIDCLL 300
           VTQYPYFL MRVGTMIKPLV+FIVSLGLPKKIVARMLEKRAYILGYDLEETV+PNIDCLL
Sbjct: 247 VTQYPYFLAMRVGTMIKPLVDFIVSLGLPKKIVARMLEKRAYILGYDLEETVRPNIDCLL 306

Query: 301 SFGIRKELLPSVIAQYPQILGLPLKAKLSSQQFFFDLKLKIDPAGFAQVIEKMPQIVSLH 360
           SFGIRKELLPSVIA YPQILGLPLKAKLS+QQFFFDLKLKIDPAGFAQVIEKMPQI+ LH
Sbjct: 307 SFGIRKELLPSVIAHYPQILGLPLKAKLSAQQFFFDLKLKIDPAGFAQVIEKMPQILCLH 366

Query: 361 QHVIIKPAEFFLGRGIPSSDVAKMIIKCPQLVACRVELMKNSYYFFKSEMGRPIKELVNF 420
           QHVI+KP EF LGRGIPSSDVAKM++KCPQLVACRVELMKNSYYFFKSEMGRPIKELV F
Sbjct: 367 QHVIMKPVEFLLGRGIPSSDVAKMVVKCPQLVACRVELMKNSYYFFKSEMGRPIKELVEF 426

Query: 421 PEYFTYSLESRIKPRYERLQSKGIRCSLNWFLNCSDQRFEERLKGEFIETESLRPSFCMG 480
           PEYFTYSLESRIKPRYER+Q KGIRCSLNWFLN S+QRFEERLKG+FIETESL PSFCMG
Sbjct: 427 PEYFTYSLESRIKPRYERVQRKGIRCSLNWFLNVSEQRFEERLKGDFIETESLGPSFCMG 486

Query: 481 GRLELPGNEIIVSDDEVEEDECDEK-LHRRIVSL 514
           GRLE+PGNE IVSDD  EEDECDE+ LHRRIVSL
Sbjct: 487 GRLEVPGNETIVSDD--EEDECDEQFLHRRIVSL 518

The following BLAST results are available for this feature:
BLAST of Bhi11G001312 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G44020.14.9e-21674.48Mitochondrial transcription termination factor family protein [more]
AT4G02990.14.7e-12650.00Mitochondrial transcription termination factor family protein [more]
AT2G21710.14.8e-3824.86Mitochondrial transcription termination factor family protein [more]
AT1G78930.13.0e-3225.54Mitochondrial transcription termination factor family protein [more]
AT4G38160.15.9e-2822.67Mitochondrial transcription termination factor family protein [more]
BLAST of Bhi11G001312 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9ZT966.6e-12550.00Transcription termination factor MTERF4, chloroplastic OS=Arabidopsis thaliana O... [more]
B6TGN42.7e-11848.11Transcription termination factor MTERF4, chloroplastic OS=Zea mays OX=4577 GN=MT... [more]
Q6AUK64.6e-11850.24Transcription termination factor MTERF4, chloroplastic OS=Oryza sativa subsp. ja... [more]
F4IHL36.8e-3724.86Transcription termination factor MTERF2, chloroplastic OS=Arabidopsis thaliana O... [more]
Q9SZL68.3e-2722.67Transcription termination factor MTERF6, chloroplastic/mitochondrial OS=Arabidop... [more]
BLAST of Bhi11G001312 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038905918.16.2e-298100.00transcription termination factor MTERF4, chloroplastic [Benincasa hispida] >XP_0... [more]
XP_004141131.11.6e-27793.37transcription termination factor MTERF4, chloroplastic [Cucumis sativus] >KGN597... [more]
XP_008464974.13.6e-27792.20PREDICTED: transcription termination factor MTERF4, chloroplastic [Cucumis melo]... [more]
XP_022980596.16.7e-27692.80transcription termination factor MTERF4, chloroplastic-like [Cucurbita maxima][more]
XP_022930565.11.4e-27392.22transcription termination factor MTERF4, chloroplastic [Cucurbita moschata][more]
BLAST of Bhi11G001312 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A0A0LI617.7e-27893.37Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G842630 PE=3 SV=1[more]
A0A5D3D5I61.7e-27792.20Transcription termination factor MTERF4 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S3CN781.7e-27792.20transcription termination factor MTERF4, chloroplastic OS=Cucumis melo OX=3656 G... [more]
A0A6J1IU103.2e-27692.80transcription termination factor MTERF4, chloroplastic-like OS=Cucurbita maxima ... [more]
A0A6J1EX706.8e-27492.22transcription termination factor MTERF4, chloroplastic OS=Cucurbita moschata OX=... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003690Transcription termination factor, mitochondrial/chloroplasticSMARTSM00733mt_12coord: 274..305
e-value: 6.8E-5
score: 32.3
coord: 238..269
e-value: 0.0033
score: 26.7
coord: 130..161
e-value: 0.0017
score: 27.6
coord: 310..342
e-value: 110.0
score: 11.2
coord: 166..197
e-value: 0.58
score: 19.2
coord: 202..233
e-value: 0.0034
score: 26.6
coord: 347..377
e-value: 130.0
score: 10.9
coord: 414..445
e-value: 1.5E-5
score: 34.4
coord: 382..413
e-value: 0.0017
score: 27.6
IPR003690Transcription termination factor, mitochondrial/chloroplasticPFAMPF02536mTERFcoord: 152..460
e-value: 4.9E-105
score: 350.9
IPR003690Transcription termination factor, mitochondrial/chloroplasticPANTHERPTHR13068CGI-12 PROTEIN-RELATEDcoord: 51..493
IPR038538MTERF superfamily, mitochondrial/chloroplasticGENE3D1.25.70.10Transcription termination factor 3, mitochondrialcoord: 119..256
e-value: 2.0E-25
score: 91.4
coord: 257..469
e-value: 1.1E-49
score: 171.0
NoneNo IPR availablePANTHERPTHR13068:SF24EXPRESSED PROTEINcoord: 51..493

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi11M001312Bhi11M001312mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032502 developmental process
biological_process GO:0006353 DNA-templated transcription, termination
biological_process GO:0006355 regulation of transcription, DNA-templated
molecular_function GO:0003690 double-stranded DNA binding