Bhi11G001246 (gene) Wax gourd (B227) v1

Overview
NameBhi11G001246
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionChaperone protein dnaJ 8
Locationchr11: 42157514 .. 42159335 (+)
RNA-Seq ExpressionBhi11G001246
SyntenyBhi11G001246
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCCGAATCTAAAATCCCTTCCCTTCTTTCTTCTTCTTCCCAATTCCATTCTATTTATTGCCCTCCCTTTCCCCTTCCTATTCCAAATCCAAATCCCTCTTTCACCTTCACTTGATTTTCTCCTTCTCTTTTGAATCTTTTTAAACCCTAAACACTTTCCTCTTCTTCTTCTGTTAATGGCGTCCGTTGCTACTGCTGGATTCATTGCCCGGAGTGGTTCTTCCTCTTCTTCGTCGTGGATTCGCCATAGGAATCGACCGAAGAAGGATCAGAGTTCAACAAATCAACTTAGGGTTTCTTGTTCTTCTTCTTCTTCTTCTCCTTCATCGGTTGTTGATCCTTATCGGACTCTTCGGATTCAACCTGGAGCCTCTGAATCTGAAGTCAAAAAGGCCTTCCGTCATCTCGCTCTTCAGGTAATTCCACAACCTATCTCGATTTCTCTTTTTTTCTTTTACAGAATTTGTTTATCTCATTCTTTCGATTTCTTCGATTTTCAGTATCATCCTGACGTTTGCAGAGGAAGTAATTGTGGTGTGCAGTTTCATCAAATCAACGAAGCTTACGATGTACGTGATCATATACGCCCCCCCCCTTTTTTTTAATTATCTACTAAAATTAGGATCAATTATTGGAATTATGAATTGGTTTTTTTTTCTCTCCCTTAAAAGAGCAGTTATTGCGATTACTGATTAAATATATAGATATCTAGAGGAAGAATACTTTGAATTTCACAATACATAGCGTGTGAAATTGAGTGTTTGGTTAGGTTGTCGGTTTCACAGAGTTGCGATCATTCTGACGTTGAATTTCCTATACCTATTTGTCGTATATAATGTTAGCTAAAATTATTTATATGTATAAGTCTTTTGCTTTTTAATGGGACCCCAGGATGATCCAAAATTGTTAGAACGTTTTTATGTTAAATGGCAAAGCAAATTTGGTCCTACCAGGAGAAATTTAGAATTTAATCATTTCTAGTTTATAATTAGAACCTAGGCCACCTTTAGCCATACCTAATAATAGGTTTTTATCGATAGATCAGTTGCAATGATCACAATCACAAACAGCAACAATATTGACATAATTTTCAAATGCTATTGAATTTAGCATTTTTTGCCCGTGAATCACACCATACCAAATCTAGGCTGGGATTATATAATTTTATTGAACTACAGGAAAAAAAAAATTCAAACTTTTTTTCAGATCATAGCTATTTTTAATCGGAATTTTTTACCTTGTAACAAATAACAGATATCAGTTATGAATATAGTTTTGTTTTTGCTTTACTAATGGGGTAAATTTTTTTGTTTGGTGAAACAGATTGTGATGAATAACTTAAGAGGAATATCGACACCAATTGAAACATATGAGACATATGATGAAGGGGTGGATGAACCGATGAGAGGAATGGAGGATCCGGATTGGGATTTATGGGAAGAATGGATGGGATGGGAAGGAGCAGGAATTCGTGATTATTCCTCTCATATTAATCCATACATTTAAAACCAAAAATTATAAGTTATTGTGAATTGTTAATAGAATTGGTCCTTAATCTATCAACATCAACGTCAACCAATGGTTGCAGTGGTTTGATTTGGTTCGATTTGGTTTGTATATATGGTGGGAAAAGAGGGGGAATTGAAAATTGATATTCATTAATGTACACCCTTTTTTTTCAAGTGTTTGTACATATGGTCAAGACAAGGCCTTTGGGTTTTTAATAATATGAAAAGGCTTTCAAGATGCCCTATCGAAGTTGTGGTGGGACATTTGTGTTTTTCTCTCTCTCTCTTTCTTGTTCTTTGCCCAATTAAACGTGG

mRNA sequence

TCCGAATCTAAAATCCCTTCCCTTCTTTCTTCTTCTTCCCAATTCCATTCTATTTATTGCCCTCCCTTTCCCCTTCCTATTCCAAATCCAAATCCCTCTTTCACCTTCACTTGATTTTCTCCTTCTCTTTTGAATCTTTTTAAACCCTAAACACTTTCCTCTTCTTCTTCTGTTAATGGCGTCCGTTGCTACTGCTGGATTCATTGCCCGGAGTGGTTCTTCCTCTTCTTCGTCGTGGATTCGCCATAGGAATCGACCGAAGAAGGATCAGAGTTCAACAAATCAACTTAGGGTTTCTTGTTCTTCTTCTTCTTCTTCTCCTTCATCGGTTGTTGATCCTTATCGGACTCTTCGGATTCAACCTGGAGCCTCTGAATCTGAAGTCAAAAAGGCCTTCCGTCATCTCGCTCTTCAGTATCATCCTGACGTTTGCAGAGGAAGTAATTGTGGTGTGCAGTTTCATCAAATCAACGAAGCTTACGATATTGTGATGAATAACTTAAGAGGAATATCGACACCAATTGAAACATATGAGACATATGATGAAGGGGTGGATGAACCGATGAGAGGAATGGAGGATCCGGATTGGGATTTATGGGAAGAATGGATGGGATGGGAAGGAGCAGGAATTCGTGATTATTCCTCTCATATTAATCCATACATTTAAAACCAAAAATTATAAGTTATTGTGAATTGTTAATAGAATTGGTCCTTAATCTATCAACATCAACGTCAACCAATGGTTGCAGTGGTTTGATTTGGTTCGATTTGGTTTGTATATATGGTGGGAAAAGAGGGGGAATTGAAAATTGATATTCATTAATGTACACCCTTTTTTTTCAAGTGTTTGTACATATGGTCAAGACAAGGCCTTTGGGTTTTTAATAATATGAAAAGGCTTTCAAGATGCCCTATCGAAGTTGTGGTGGGACATTTGTGTTTTTCTCTCTCTCTCTTTCTTGTTCTTTGCCCAATTAAACGTGG

Coding sequence (CDS)

ATGGCGTCCGTTGCTACTGCTGGATTCATTGCCCGGAGTGGTTCTTCCTCTTCTTCGTCGTGGATTCGCCATAGGAATCGACCGAAGAAGGATCAGAGTTCAACAAATCAACTTAGGGTTTCTTGTTCTTCTTCTTCTTCTTCTCCTTCATCGGTTGTTGATCCTTATCGGACTCTTCGGATTCAACCTGGAGCCTCTGAATCTGAAGTCAAAAAGGCCTTCCGTCATCTCGCTCTTCAGTATCATCCTGACGTTTGCAGAGGAAGTAATTGTGGTGTGCAGTTTCATCAAATCAACGAAGCTTACGATATTGTGATGAATAACTTAAGAGGAATATCGACACCAATTGAAACATATGAGACATATGATGAAGGGGTGGATGAACCGATGAGAGGAATGGAGGATCCGGATTGGGATTTATGGGAAGAATGGATGGGATGGGAAGGAGCAGGAATTCGTGATTATTCCTCTCATATTAATCCATACATTTAA

Protein sequence

MASVATAGFIARSGSSSSSSWIRHRNRPKKDQSSTNQLRVSCSSSSSSPSSVVDPYRTLRIQPGASESEVKKAFRHLALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPIETYETYDEGVDEPMRGMEDPDWDLWEEWMGWEGAGIRDYSSHINPYI
Homology
BLAST of Bhi11G001246 vs. TAIR 10
Match: AT1G80920.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 177.9 bits (450), Expect = 6.4e-45
Identity = 93/157 (59.24%), Postives = 111/157 (70.70%), Query Frame = 0

Query: 8   GFIARSGS--SSSSSWIRHRNRPKKDQSSTNQLRVSCSSSSSSPSSVVDPYRTLRIQPGA 67
           GF    GS  SSSSS  R +N  +K+    N+ +V CSSS    SSV+DPY+TL+I+P +
Sbjct: 11  GFSGLPGSSFSSSSSSFRLKNSRRKNTKMLNRSKVVCSSS----SSVMDPYKTLKIRPDS 70

Query: 68  SESEVKKAFRHLALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPIETYETYDEG 127
           SE EVKKAFR LA +YHPDVCRGSNCGVQF  INEAYDIV+  ++      E +E +D  
Sbjct: 71  SEYEVKKAFRQLAKKYHPDVCRGSNCGVQFQTINEAYDIVLKQIKNQMEGTEEFEPFDV- 130

Query: 128 VDEPMRGMEDPDWDLWEEWMGWEGAGIRDYSSHINPY 163
            DE + GM DPD D WEEWMGWEGAG RDYSSH+NPY
Sbjct: 131 YDEGLNGMNDPDCDTWEEWMGWEGAGTRDYSSHVNPY 162

BLAST of Bhi11G001246 vs. TAIR 10
Match: AT1G72070.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 63.2 bits (152), Expect = 2.3e-10
Identity = 36/94 (38.30%), Postives = 51/94 (54.26%), Query Frame = 0

Query: 56  YRTLRIQPGASESEVKKAFRHLALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTP 115
           Y  L + P AS++EVK+AF+ LAL+YHPDV +G +    F +I  AY+ +M   +     
Sbjct: 41  YTVLGLTPLASQTEVKRAFKRLALKYHPDVHKGQD--KDFKEIKSAYECLMQKFK----- 100

Query: 116 IETYETYDEGVDEPMRGMEDPDWDLWEEWMGWEG 150
                      +E M   E  + D WEEWMG+EG
Sbjct: 101 ---------KEEEEMEITEMGEIDEWEEWMGFEG 118

BLAST of Bhi11G001246 vs. TAIR 10
Match: AT5G59610.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 60.5 bits (145), Expect = 1.5e-09
Identity = 36/107 (33.64%), Postives = 56/107 (52.34%), Query Frame = 0

Query: 55  PYRTLRIQPGASESEVKKAFRHLALQYHPDVCRGSNCGVQFHQINEAYDIVMNN------ 114
           PY  L + P A+  ++K+A+R LAL+YHPDV + +N   +F +I  AY  ++N+      
Sbjct: 74  PYEILGVSPSATPQDIKRAYRKLALKYHPDVNKEANAQEKFLKIKHAYTTLINSDSRRKY 133

Query: 115 -----LRGISTPIETYETYDEGVDEPMRGMEDPDWDLWEEWMGWEGA 151
                  G ST  +T    +  V+E   G+ D   DL EE+  WE +
Sbjct: 134 GSDSRATGSSTG-QTSRKGNSQVEEDFYGLGDFFKDLQEEYKNWEAS 179

BLAST of Bhi11G001246 vs. TAIR 10
Match: AT1G80030.1 (Molecular chaperone Hsp40/DnaJ family protein )

HSP 1 Score: 53.9 bits (128), Expect = 1.4e-07
Identity = 43/126 (34.13%), Postives = 63/126 (50.00%), Query Frame = 0

Query: 38  LRVSCSSSSSSP-------SSVV-----DPYRTLRIQPGASESEVKKAFRHLALQYHPDV 97
           L +S SSSSS+P       S VV     D Y TL +   A+  E+K A+R LA QYHPDV
Sbjct: 47  LSLSSSSSSSTPYRNRRGRSLVVFATSGDYYATLGVSKSANNKEIKAAYRRLARQYHPDV 106

Query: 98  CRGSNCGVQFHQINEAYDIVMNNLRGISTPIETYETYDE-GVDEPMRGMEDP----DWDL 147
            +      +F +I+ AY+++ +  +        Y+ Y E GV   + G   P     +DL
Sbjct: 107 NKEPGATEKFKEISAAYEVLSDEQKR-----ALYDQYGEAGVKSTVGGASGPYTSNPFDL 166

BLAST of Bhi11G001246 vs. TAIR 10
Match: AT1G80030.3 (Molecular chaperone Hsp40/DnaJ family protein )

HSP 1 Score: 53.9 bits (128), Expect = 1.4e-07
Identity = 43/126 (34.13%), Postives = 63/126 (50.00%), Query Frame = 0

Query: 38  LRVSCSSSSSSP-------SSVV-----DPYRTLRIQPGASESEVKKAFRHLALQYHPDV 97
           L +S SSSSS+P       S VV     D Y TL +   A+  E+K A+R LA QYHPDV
Sbjct: 47  LSLSSSSSSSTPYRNRRGRSLVVFATSGDYYATLGVSKSANNKEIKAAYRRLARQYHPDV 106

Query: 98  CRGSNCGVQFHQINEAYDIVMNNLRGISTPIETYETYDE-GVDEPMRGMEDP----DWDL 147
            +      +F +I+ AY+++ +  +        Y+ Y E GV   + G   P     +DL
Sbjct: 107 NKEPGATEKFKEISAAYEVLSDEQKR-----ALYDQYGEAGVKSTVGGASGPYTSNPFDL 166

BLAST of Bhi11G001246 vs. ExPASy Swiss-Prot
Match: Q9SAG8 (Chaperone protein dnaJ 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ATJ8 PE=2 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 9.0e-44
Identity = 93/157 (59.24%), Postives = 111/157 (70.70%), Query Frame = 0

Query: 8   GFIARSGS--SSSSSWIRHRNRPKKDQSSTNQLRVSCSSSSSSPSSVVDPYRTLRIQPGA 67
           GF    GS  SSSSS  R +N  +K+    N+ +V CSSS    SSV+DPY+TL+I+P +
Sbjct: 11  GFSGLPGSSFSSSSSSFRLKNSRRKNTKMLNRSKVVCSSS----SSVMDPYKTLKIRPDS 70

Query: 68  SESEVKKAFRHLALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPIETYETYDEG 127
           SE EVKKAFR LA +YHPDVCRGSNCGVQF  INEAYDIV+  ++      E +E +D  
Sbjct: 71  SEYEVKKAFRQLAKKYHPDVCRGSNCGVQFQTINEAYDIVLKQIKNQMEGTEEFEPFDV- 130

Query: 128 VDEPMRGMEDPDWDLWEEWMGWEGAGIRDYSSHINPY 163
            DE + GM DPD D WEEWMGWEGAG RDYSSH+NPY
Sbjct: 131 YDEGLNGMNDPDCDTWEEWMGWEGAGTRDYSSHVNPY 162

BLAST of Bhi11G001246 vs. ExPASy Swiss-Prot
Match: A7NS65 (Chaperone protein DnaJ OS=Roseiflexus castenholzii (strain DSM 13941 / HLO8) OX=383372 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 3.6e-08
Identity = 26/52 (50.00%), Postives = 36/52 (69.23%), Query Frame = 0

Query: 54  DPYRTLRIQPGASESEVKKAFRHLALQYHPDVCRGSNCGVQFHQINEAYDIV 106
           D Y  L +Q  AS+ E+KKAFR LA QYHPDV +  +   +F +INEAY+++
Sbjct: 6   DYYEVLGVQRNASQDEIKKAFRRLARQYHPDVNKAPDAEAKFKEINEAYEVL 57

BLAST of Bhi11G001246 vs. ExPASy Swiss-Prot
Match: A5UYW4 (Chaperone protein DnaJ OS=Roseiflexus sp. (strain RS-1) OX=357808 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 3.6e-08
Identity = 26/52 (50.00%), Postives = 36/52 (69.23%), Query Frame = 0

Query: 54  DPYRTLRIQPGASESEVKKAFRHLALQYHPDVCRGSNCGVQFHQINEAYDIV 106
           D Y  L +Q  AS+ E+KKAFR LA QYHPDV +  +   +F +INEAY+++
Sbjct: 6   DYYEVLGVQRNASQDEIKKAFRRLARQYHPDVNKAPDAEAKFKEINEAYEVL 57

BLAST of Bhi11G001246 vs. ExPASy Swiss-Prot
Match: Q8G6C6 (Chaperone protein DnaJ OS=Bifidobacterium longum (strain NCC 2705) OX=206672 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 6.1e-08
Identity = 37/107 (34.58%), Postives = 53/107 (49.53%), Query Frame = 0

Query: 52  VVDPYRTLRIQPGASESEVKKAFRHLALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRG 111
           + D Y TL ++ GAS+ E+KKA+R L+ +YHPD+  G     +F ++N AYD++ N    
Sbjct: 1   MADYYETLGVERGASDDEIKKAYRKLSRKYHPDIA-GPEFEDKFKEVNNAYDVLSNP--- 60

Query: 112 ISTPIETYETYDEGVD--EPMRGMEDPDWDLWEEWMGWEGAGIRDYS 157
                +    YD GVD   P  G             G+ GAG  D S
Sbjct: 61  -----DKRRMYDSGVDPNNPNAGAG-----------GFSGAGFGDMS 87

BLAST of Bhi11G001246 vs. ExPASy Swiss-Prot
Match: Q65U54 (Chaperone protein DnaJ OS=Mannheimia succiniciproducens (strain MBEL55E) OX=221988 GN=dnaJ PE=3 SV=2)

HSP 1 Score: 58.9 bits (141), Expect = 6.1e-08
Identity = 26/73 (35.62%), Postives = 45/73 (61.64%), Query Frame = 0

Query: 54  DPYRTLRIQPGASESEVKKAFRHLALQYHPDVCRGSNCGVQ-FHQINEAYDIVMNNLRGI 113
           D Y TL +Q GA E E+K+A++ LA++YHPD   G     + F ++NEAY+I+M+  +  
Sbjct: 5   DYYETLGVQKGADEKEIKRAYKRLAMKYHPDRTNGDKAAEEKFKEVNEAYEILMDKEKRA 64

Query: 114 STPIETYETYDEG 126
           +     +  +++G
Sbjct: 65  AYDQYGHAAFEQG 77

BLAST of Bhi11G001246 vs. NCBI nr
Match: XP_038906426.1 (chaperone protein dnaJ 8, chloroplastic [Benincasa hispida])

HSP 1 Score: 335.1 bits (858), Expect = 3.4e-88
Identity = 163/163 (100.00%), Postives = 163/163 (100.00%), Query Frame = 0

Query: 1   MASVATAGFIARSGSSSSSSWIRHRNRPKKDQSSTNQLRVSCSSSSSSPSSVVDPYRTLR 60
           MASVATAGFIARSGSSSSSSWIRHRNRPKKDQSSTNQLRVSCSSSSSSPSSVVDPYRTLR
Sbjct: 1   MASVATAGFIARSGSSSSSSWIRHRNRPKKDQSSTNQLRVSCSSSSSSPSSVVDPYRTLR 60

Query: 61  IQPGASESEVKKAFRHLALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPIETYE 120
           IQPGASESEVKKAFRHLALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPIETYE
Sbjct: 61  IQPGASESEVKKAFRHLALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPIETYE 120

Query: 121 TYDEGVDEPMRGMEDPDWDLWEEWMGWEGAGIRDYSSHINPYI 164
           TYDEGVDEPMRGMEDPDWDLWEEWMGWEGAGIRDYSSHINPYI
Sbjct: 121 TYDEGVDEPMRGMEDPDWDLWEEWMGWEGAGIRDYSSHINPYI 163

BLAST of Bhi11G001246 vs. NCBI nr
Match: XP_008465135.1 (PREDICTED: chaperone protein dnaJ 8, chloroplastic [Cucumis melo] >TYK27005.1 chaperone protein dnaJ 8 [Cucumis melo var. makuwa])

HSP 1 Score: 290.8 bits (743), Expect = 7.3e-75
Identity = 148/165 (89.70%), Postives = 154/165 (93.33%), Query Frame = 0

Query: 1   MASVATAGFIAR-SGSSSSSSWIRHRNRPKKDQSSTNQLRVSCSSSSSSPSSVVDPYRTL 60
           MASVATAGFIAR S SSSSSS IR+ NRPKK+++S+NQLRVSCSSS    SSV DPYRTL
Sbjct: 1   MASVATAGFIARSSSSSSSSSCIRNTNRPKKNRTSSNQLRVSCSSS----SSVADPYRTL 60

Query: 61  RIQPGASESEVKKAFRHLALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPIETY 120
           RIQPGASESEVKKAFRHLALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPIETY
Sbjct: 61  RIQPGASESEVKKAFRHLALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPIETY 120

Query: 121 ET-YDEGVDEPMRGMEDPDWDLWEEWMGWEGAGIRDYSSHINPYI 164
           ET YDEG+DEPMRGM DPDWDLWEEWMGWEGAGIRDYSSHINPYI
Sbjct: 121 ETIYDEGIDEPMRGMGDPDWDLWEEWMGWEGAGIRDYSSHINPYI 161

BLAST of Bhi11G001246 vs. NCBI nr
Match: XP_022983187.1 (chaperone protein dnaJ 8, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 275.0 bits (702), Expect = 4.1e-70
Identity = 137/164 (83.54%), Postives = 148/164 (90.24%), Query Frame = 0

Query: 1   MASVATAGF-IARSGSSSSSSWIRHRNRPKKDQSSTNQLRVSCSSSSSSPSSVVDPYRTL 60
           MASVATAG  IAR+GSSSSS WIRHRNR  KD+SS N++RV+CS      SS++DPY+TL
Sbjct: 1   MASVATAGISIARTGSSSSSFWIRHRNRSNKDRSSRNRVRVTCS------SSLLDPYQTL 60

Query: 61  RIQPGASESEVKKAFRHLALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPIETY 120
           RIQPGASESEVKKAFR LALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPIET 
Sbjct: 61  RIQPGASESEVKKAFRRLALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPIETC 120

Query: 121 ETYDEGVDEPMRGMEDPDWDLWEEWMGWEGAGIRDYSSHINPYI 164
           E+ +EGVDE MRGMEDPDW+LWEEWMGWEGAGIRDYSSHINPYI
Sbjct: 121 ESCEEGVDEAMRGMEDPDWELWEEWMGWEGAGIRDYSSHINPYI 158

BLAST of Bhi11G001246 vs. NCBI nr
Match: XP_022934104.1 (chaperone protein dnaJ 8, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 271.2 bits (692), Expect = 6.0e-69
Identity = 137/166 (82.53%), Postives = 149/166 (89.76%), Query Frame = 0

Query: 1   MASVATAGF-IARSGSSSSSS--WIRHRNRPKKDQSSTNQLRVSCSSSSSSPSSVVDPYR 60
           MASVATAG  IAR+GSSSSSS  WIRHRNR  KD+SS N++RV+CS      SS++DPY+
Sbjct: 1   MASVATAGISIARTGSSSSSSSFWIRHRNRSNKDRSSRNRVRVTCS------SSLLDPYQ 60

Query: 61  TLRIQPGASESEVKKAFRHLALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPIE 120
           TLRIQPGASESEVKKAFR LALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPIE
Sbjct: 61  TLRIQPGASESEVKKAFRRLALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPIE 120

Query: 121 TYETYDEGVDEPMRGMEDPDWDLWEEWMGWEGAGIRDYSSHINPYI 164
           T E+ +EGVDE MRGM+DPDW+LWEEWMGWEGAGIRDYSSHINPYI
Sbjct: 121 TRESCEEGVDEEMRGMDDPDWELWEEWMGWEGAGIRDYSSHINPYI 160

BLAST of Bhi11G001246 vs. NCBI nr
Match: XP_023526464.1 (chaperone protein dnaJ 8, chloroplastic-like [Cucurbita pepo subsp. pepo] >KAG6581620.1 Chaperone protein dnaJ 8, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018119.1 Chaperone protein dnaJ 8, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 271.2 bits (692), Expect = 6.0e-69
Identity = 137/166 (82.53%), Postives = 149/166 (89.76%), Query Frame = 0

Query: 1   MASVATAGF-IARSGSSSSSS--WIRHRNRPKKDQSSTNQLRVSCSSSSSSPSSVVDPYR 60
           MASVATAG  IAR+GSSSSSS  WIRHRNR  KD+SS N++RV+CS      SS++DPY+
Sbjct: 1   MASVATAGISIARTGSSSSSSSFWIRHRNRSNKDRSSRNRVRVTCS------SSLLDPYQ 60

Query: 61  TLRIQPGASESEVKKAFRHLALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPIE 120
           TLRIQPGASESEVKKAFR LALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPIE
Sbjct: 61  TLRIQPGASESEVKKAFRRLALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPIE 120

Query: 121 TYETYDEGVDEPMRGMEDPDWDLWEEWMGWEGAGIRDYSSHINPYI 164
           T E+ +EGVDE MRGM+DPDW+LWEEWMGWEGAGIRDYSSHINPYI
Sbjct: 121 TRESCEEGVDEAMRGMDDPDWELWEEWMGWEGAGIRDYSSHINPYI 160

BLAST of Bhi11G001246 vs. ExPASy TrEMBL
Match: A0A5D3DUL4 (Chaperone protein dnaJ 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold901G00100 PE=4 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 3.5e-75
Identity = 148/165 (89.70%), Postives = 154/165 (93.33%), Query Frame = 0

Query: 1   MASVATAGFIAR-SGSSSSSSWIRHRNRPKKDQSSTNQLRVSCSSSSSSPSSVVDPYRTL 60
           MASVATAGFIAR S SSSSSS IR+ NRPKK+++S+NQLRVSCSSS    SSV DPYRTL
Sbjct: 1   MASVATAGFIARSSSSSSSSSCIRNTNRPKKNRTSSNQLRVSCSSS----SSVADPYRTL 60

Query: 61  RIQPGASESEVKKAFRHLALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPIETY 120
           RIQPGASESEVKKAFRHLALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPIETY
Sbjct: 61  RIQPGASESEVKKAFRHLALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPIETY 120

Query: 121 ET-YDEGVDEPMRGMEDPDWDLWEEWMGWEGAGIRDYSSHINPYI 164
           ET YDEG+DEPMRGM DPDWDLWEEWMGWEGAGIRDYSSHINPYI
Sbjct: 121 ETIYDEGIDEPMRGMGDPDWDLWEEWMGWEGAGIRDYSSHINPYI 161

BLAST of Bhi11G001246 vs. ExPASy TrEMBL
Match: A0A1S3CPN7 (chaperone protein dnaJ 8, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103502812 PE=4 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 3.5e-75
Identity = 148/165 (89.70%), Postives = 154/165 (93.33%), Query Frame = 0

Query: 1   MASVATAGFIAR-SGSSSSSSWIRHRNRPKKDQSSTNQLRVSCSSSSSSPSSVVDPYRTL 60
           MASVATAGFIAR S SSSSSS IR+ NRPKK+++S+NQLRVSCSSS    SSV DPYRTL
Sbjct: 1   MASVATAGFIARSSSSSSSSSCIRNTNRPKKNRTSSNQLRVSCSSS----SSVADPYRTL 60

Query: 61  RIQPGASESEVKKAFRHLALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPIETY 120
           RIQPGASESEVKKAFRHLALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPIETY
Sbjct: 61  RIQPGASESEVKKAFRHLALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPIETY 120

Query: 121 ET-YDEGVDEPMRGMEDPDWDLWEEWMGWEGAGIRDYSSHINPYI 164
           ET YDEG+DEPMRGM DPDWDLWEEWMGWEGAGIRDYSSHINPYI
Sbjct: 121 ETIYDEGIDEPMRGMGDPDWDLWEEWMGWEGAGIRDYSSHINPYI 161

BLAST of Bhi11G001246 vs. ExPASy TrEMBL
Match: A0A6J1IYK7 (chaperone protein dnaJ 8, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111481844 PE=4 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 2.0e-70
Identity = 137/164 (83.54%), Postives = 148/164 (90.24%), Query Frame = 0

Query: 1   MASVATAGF-IARSGSSSSSSWIRHRNRPKKDQSSTNQLRVSCSSSSSSPSSVVDPYRTL 60
           MASVATAG  IAR+GSSSSS WIRHRNR  KD+SS N++RV+CS      SS++DPY+TL
Sbjct: 1   MASVATAGISIARTGSSSSSFWIRHRNRSNKDRSSRNRVRVTCS------SSLLDPYQTL 60

Query: 61  RIQPGASESEVKKAFRHLALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPIETY 120
           RIQPGASESEVKKAFR LALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPIET 
Sbjct: 61  RIQPGASESEVKKAFRRLALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPIETC 120

Query: 121 ETYDEGVDEPMRGMEDPDWDLWEEWMGWEGAGIRDYSSHINPYI 164
           E+ +EGVDE MRGMEDPDW+LWEEWMGWEGAGIRDYSSHINPYI
Sbjct: 121 ESCEEGVDEAMRGMEDPDWELWEEWMGWEGAGIRDYSSHINPYI 158

BLAST of Bhi11G001246 vs. ExPASy TrEMBL
Match: A0A6J1F1M8 (chaperone protein dnaJ 8, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111441378 PE=4 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 2.9e-69
Identity = 137/166 (82.53%), Postives = 149/166 (89.76%), Query Frame = 0

Query: 1   MASVATAGF-IARSGSSSSSS--WIRHRNRPKKDQSSTNQLRVSCSSSSSSPSSVVDPYR 60
           MASVATAG  IAR+GSSSSSS  WIRHRNR  KD+SS N++RV+CS      SS++DPY+
Sbjct: 1   MASVATAGISIARTGSSSSSSSFWIRHRNRSNKDRSSRNRVRVTCS------SSLLDPYQ 60

Query: 61  TLRIQPGASESEVKKAFRHLALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPIE 120
           TLRIQPGASESEVKKAFR LALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPIE
Sbjct: 61  TLRIQPGASESEVKKAFRRLALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPIE 120

Query: 121 TYETYDEGVDEPMRGMEDPDWDLWEEWMGWEGAGIRDYSSHINPYI 164
           T E+ +EGVDE MRGM+DPDW+LWEEWMGWEGAGIRDYSSHINPYI
Sbjct: 121 TRESCEEGVDEEMRGMDDPDWELWEEWMGWEGAGIRDYSSHINPYI 160

BLAST of Bhi11G001246 vs. ExPASy TrEMBL
Match: A0A6J1GC69 (chaperone protein dnaJ 8, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111452806 PE=4 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 8.4e-69
Identity = 137/164 (83.54%), Postives = 145/164 (88.41%), Query Frame = 0

Query: 1   MASVATAGFIARSGSSSSSSWIRHRNRPKK-DQSSTNQLRVSCSSSSSSPSSVVDPYRTL 60
           MASV+TAGFIAR+G   SSSWIR RNR  K  +SS NQ+R S SS SS  SS+ DPYRTL
Sbjct: 1   MASVSTAGFIARTG---SSSWIRQRNRANKLHRSSRNQVRASYSSYSS--SSMADPYRTL 60

Query: 61  RIQPGASESEVKKAFRHLALQYHPDVCRGSNCGVQFHQINEAYDIVMNNLRGISTPIETY 120
           RIQPGASESEVKKAFR LALQYHPDVC+GSNCGVQFHQINEAYDIVMNNLRGIS PIETY
Sbjct: 61  RIQPGASESEVKKAFRQLALQYHPDVCKGSNCGVQFHQINEAYDIVMNNLRGISMPIETY 120

Query: 121 ETYDEGVDEPMRGMEDPDWDLWEEWMGWEGAGIRDYSSHINPYI 164
           +TYD+G DEPMRGM DPDWDLWEEWMGWEGAGIRDYSSHINPYI
Sbjct: 121 DTYDDGGDEPMRGMGDPDWDLWEEWMGWEGAGIRDYSSHINPYI 159

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT1G80920.16.4e-4559.24Chaperone DnaJ-domain superfamily protein [more]
AT1G72070.12.3e-1038.30Chaperone DnaJ-domain superfamily protein [more]
AT5G59610.11.5e-0933.64Chaperone DnaJ-domain superfamily protein [more]
AT1G80030.11.4e-0734.13Molecular chaperone Hsp40/DnaJ family protein [more]
AT1G80030.31.4e-0734.13Molecular chaperone Hsp40/DnaJ family protein [more]
Match NameE-valueIdentityDescription
Q9SAG89.0e-4459.24Chaperone protein dnaJ 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ATJ8 ... [more]
A7NS653.6e-0850.00Chaperone protein DnaJ OS=Roseiflexus castenholzii (strain DSM 13941 / HLO8) OX=... [more]
A5UYW43.6e-0850.00Chaperone protein DnaJ OS=Roseiflexus sp. (strain RS-1) OX=357808 GN=dnaJ PE=3 S... [more]
Q8G6C66.1e-0834.58Chaperone protein DnaJ OS=Bifidobacterium longum (strain NCC 2705) OX=206672 GN=... [more]
Q65U546.1e-0835.62Chaperone protein DnaJ OS=Mannheimia succiniciproducens (strain MBEL55E) OX=2219... [more]
Match NameE-valueIdentityDescription
XP_038906426.13.4e-88100.00chaperone protein dnaJ 8, chloroplastic [Benincasa hispida][more]
XP_008465135.17.3e-7589.70PREDICTED: chaperone protein dnaJ 8, chloroplastic [Cucumis melo] >TYK27005.1 ch... [more]
XP_022983187.14.1e-7083.54chaperone protein dnaJ 8, chloroplastic-like [Cucurbita maxima][more]
XP_022934104.16.0e-6982.53chaperone protein dnaJ 8, chloroplastic-like [Cucurbita moschata][more]
XP_023526464.16.0e-6982.53chaperone protein dnaJ 8, chloroplastic-like [Cucurbita pepo subsp. pepo] >KAG65... [more]
Match NameE-valueIdentityDescription
A0A5D3DUL43.5e-7589.70Chaperone protein dnaJ 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A1S3CPN73.5e-7589.70chaperone protein dnaJ 8, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103502812 ... [more]
A0A6J1IYK72.0e-7083.54chaperone protein dnaJ 8, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1F1M82.9e-6982.53chaperone protein dnaJ 8, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1GC698.4e-6983.54chaperone protein dnaJ 8, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001623DnaJ domainPRINTSPR00625JDOMAINcoord: 90..110
score: 23.18
coord: 56..74
score: 39.56
coord: 74..89
score: 44.07
IPR001623DnaJ domainSMARTSM00271dnaj_3coord: 53..110
e-value: 8.2E-17
score: 71.9
IPR001623DnaJ domainPFAMPF00226DnaJcoord: 54..107
e-value: 1.2E-14
score: 54.2
IPR001623DnaJ domainPROSITEPS50076DNAJ_2coord: 54..123
score: 14.104311
IPR001623DnaJ domainCDDcd06257DnaJcoord: 54..104
e-value: 4.95768E-16
score: 66.0297
IPR036869Chaperone J-domain superfamilyGENE3D1.10.287.110DnaJ domaincoord: 23..111
e-value: 1.2E-17
score: 65.6
IPR036869Chaperone J-domain superfamilySUPERFAMILY46565Chaperone J-domaincoord: 51..149
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 33..55
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..55
NoneNo IPR availablePANTHERPTHR24074CO-CHAPERONE PROTEIN DJLAcoord: 5..163
NoneNo IPR availablePANTHERPTHR24074:SF37HEAT SHOCK PROTEIN BINDING PROTEINcoord: 5..163

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi11M001246Bhi11M001246mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009416 response to light stimulus
cellular_component GO:0009570 chloroplast stroma
cellular_component GO:0005634 nucleus