Bhi11G001234 (gene) Wax gourd (B227) v1

Overview
NameBhi11G001234
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Description4-coumarate--CoA ligase 1
Locationchr11: 41810962 .. 41816469 (-)
RNA-Seq ExpressionBhi11G001234
SyntenyBhi11G001234
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTGAACTCAGAAGAACAATAAAACCTTCAAAATAAAAAAAATAATAATAAAAAAAAAAGTTTTATCTGTTCTTCTTCTTCTTCTTTGGGCATTGAAATGGGCATTGAAACAGTTCAGAATGACGTCATTTTCCGGTCAAAACTACCGGACATCTACATTCCAAAGCACCTTCCTCTGCATTCCTACTGCCTACACGAAAACGCCGCCAAAATCGGCGACCGGACTTGTCTAATCAACGGCGTCACCGGCGAATCCTTCACCTACAACGATGTCGATCTCTCCGCTCGCAAAGTCGCCGCCGGACTAAACAAACTCGGAATTACAAAAAGCGATGTAATTATGCTCCTCCTCCCAAATTCGCCGGAATTCGTCTTCACTTTTCTCGGTGCCTCATACCTCGGCGCAATTATGACGGCGGCGAATCCTTTCTTCACAGCACAAGAAATCGCAAAGCAAGCAAAAGGATCGAAAGCGAAATTGATCCTAACACACTCATCATATTACGAAAAAGTAAAGGAAATAACAGAGGAATTACCTGACGTCAAAATCATGACCGTCGATTCTCCACCGGACGGTTGTTTATGGTTCGGCGATCTAATTAAAGCCGACGAACGAGATATTCCGACGGTAGAGATCGATCCAGACGACGTCGTTGCGCTGCCGTATTCCTCTGGAACTACCGGATTACCGAAAGGAGTGATGCTCACACATAAAAGTTTGGTTACGAGTGTGGCTCAGCAAGTGGATGGTGAAAATCCGAATCTGTATTATCGAAATGAGGATGTGATTCTGTGCGTTTTACCGTTGTTTCATATTTATTCGCTTAATTCGGTTTTGCTCTGTGGATTACGAGCTGGAACTACGATTTTGATTATGCCGAAATTTGAGATCGGTTTGCTTTTACAATTGGTGGAGAAATATAGAGTGTCGATTGCGCCGATTGTCCCACCGATTGTTTTGGCAATTGCGAAGTCGCCGGAGCTTGAGAAGTATGATCTTTCGTCGATCAGAGTGATTAAATGCGGCGGCGCTCCGCTTGGGAAGGAGCTTGAAGATACCGTGAGAGCTAAGTTTCCTAAGGCTGTGCTCGGCCAGGTACTACAATTTCATAATTTTTAATAATTTTAATTATCGGATATTTTTTACATACATATTAGATTAAACACCCGAGATAGAAGAACCAAACCTCCACATACTTTTAATTATCGGTTTACTTCATCATCTTTTCATGTTGAGAAAATTTAAATTTTGTCTCATATATTTTTTAAATCTTAAATTTGGCGTGACTTTCAATTGTTCTATGATTTTATTAAAAGGTTTAATACAAATATTTATTTGACATTTTAATTTTAAAAGAGAAAAAAAAAAAAAAAAAAACTTCAAGCCCATCGTATGTTGTTCAAATTCCTTTTTATTATTATTATTATTATTAAAATATATTTTTCTCAATTTTTCTATATGTGTTGAAATGAGATGCAAAAAAGACGGGGGGGGTTACACACAAGTTTGACCCACCTTTTAAATTTTAGGAATAAGTTGAAAATTCTAAGGTTTATTACTATTTTTAAAGCTTTAACATTCATTCAATCTCTGTGATTTTAGTTGAAACTTAGTTATTATGATTTTATAAAATCTTATAAATTATCTTTATAATTTGATAAAATGTTCGTACATAATCTCTACCATATCAACTATTTATGTGTATTTTATCGAATCGTAGAGACTAAATTATAATTTTTTTAAATATTGGAAACCAAATTTATAATTTAAATTTTTTAAGTTAAAAAATATATTTAAAGGTTAGATATTAAATTTATAATTTAATCTTTAATAATTAATAGTATAGTTTAAGGATAATTTACATGTATTTATTTTATTGAAAAAGGACTCAGATTTAATCATGGAGATAGTGTTTATTTAATGTAATTAAGATTCTTTTAGTTGGTATTTAGTAAAGAAACATCTTTTTTTATATATAATGATAATTAGTCAAAATTGATGATGTGAAGAGAGACTTGACTAGAGACTAAATGACACCTAAGTCAAATCAAAAGACTCCATGTAATTTTTTTTTTTTTTATTTATTATTATTTTATTATTTTATTATTATTATTATTATTATTATTATTATTTTTGCATTTCCTTATTTGTACTTGAAAGGTTTCGTCCACCTAAACAACCACTCACCAACAACAAAAAGCAAAAATCAACTTGGTAAAGTTTTCACCTAAAAGCTTAGAATAAAAAATAGGAAGTTGAATCAAAGTACTTTTGAGTTTTAACACAATTTTTACTTGGTCAGAATATCGCTTTCATACGATGGGAGTTATTGGTTCAAATGTCTTTGCTTTCAGACGTTATTTACTAATAAGAATAGGAAAAAGATAAGAGTGGAGGTTCTAACATCTCTTCTGACATTGAGTTACACTAAAACTAATTTCATCTTCTTACGCCAATTAAAAAATATGATTGTTTTTAAATAAAAAAAAATATAACAAAATATTATTAGTAAAAGACCACAATAGACATATAGTGATAGATGTCTATCCTAATCCATCACTAGTAAACAATGACATACTTGAAAATATTTTTAAAAGTTTTACCATTTAAAATAATTACCCTAAAAATATATAACAAATGTGGAAATTAATAAGTGTTATCTTCAAATTAATTGTATGATTAATGGGGAAAAGTTTTTTAAAATTCATGAACCTACTAAATGAAAAAATTGGAAGATTAGAGTTCTATTAAACATAAAATTTAACTTTTTATCTTAATGTTTTAAAATTTAAATATGTAAGACTTATTAGACAAAAGACATTGAAATTTTAAATTCATTCAATAATGATTGGGTTTTTGGAAAGTATTTATAAATATTACTTCCATCCATAAATTTACGTGAGCCGCTTTTTTCAAACATTTTATAAATCCAATGATTTTAAAAAACTAAAAGAAAATTGTCATAAACTTAGGATATGTTCAGTAGAGAATTTGAAAATAATGGATTCAATGAAAAGAGTTGTATTTTATATTTTTAAATATATGTTTGATAGTAAATTCAAAAATTAGGTTCTAATTTAAATAATTGTTTAAATTTTTTTTAATATTATTTTATAAATCATAAAATTAGTCGTAGTTATCTATGTAAATATTAGTAGACAATAAATTATTATTAAATTATTTATGAACATATATATTGTATGTTAAAAAATTTATTTAATTATTATTTTGTAATATTATATAATTTACCATACATGATATAATACATATTTTAATTTTAAAAGATTAGTTAAGTTTATAATATGACATACTATATTTTTAAATGCTCATATATTTATTTAAAATAATTATGTGCTTTAAAATTTAAAATTTAAAATGTTGATACAACAAAAACATAAAAATGTTATCTTTAGAAATGGCATGGTTTGAATCACAATTTTCTATCAAATACATATTTCTTAAACTTTGAATCTAAAAGTATAAAACATAATCCATATATAACGAATGTAAAATTTATTGAGAGAAAACAAACCAAATAAAATTTATTGAACATTTTAAAAGTTAAGAACCGTTTAGACACAATTTAAATTTGGGAGGATTGAACTTTAAATTAATGAAAATTCATGTTTAGAGAAATATTTTTCAACGTGGCAGAATGGTAGTCATTGGATCATAAAGATTTGATATAGAGAGAAATTACGTTGATTGTAGGGGTACGGAATGACGGAGGCGGGTCCAGTACTAACAATGGGTTTAGCATTTGCAAAGGAGCCATTTCCGATGAAACCAGGCGCCTGCGGCACCGTCGTCAGAAACGCCGAGATGAAGATCGTCGACACCGAAACCGGCAGCTCATTGCCTCGAAACACGCCGGGAGAGATCTGCATCAGAGGCGATCAAATCATGAAAGGGTATCTCAACAATCCGGAGGCGACGGCGGCCACAATCGACAAGGACGGCTGGTTGCACACCGGCGATATCGGATTCATCGACGACGAAGACGAGCTCTTCATTGTGGATCGGTTGAAGGAACTGATAAAGTACAAGGGATTTCAAGTGGCTCCGGCGGAACTGGAAGCTCTGTTGCTGACTCATCCGGTGATCTCTGATGCCGCCGTCGTACCGTAAGAATCAATTGAATTTGGATTCCGATTAGTGAAAAGTCGTCCATGGGTTTATTTATTATTATTATTTTTTTTTTGGTGTGTGAATTGATTTGTAGAATGAAAGATGAACAAGCTGGAGAGGTTCCCGTTGCGTTCGTTGTGAAATTGAAGAATTCTGAAGTAACTGAGGATGAAATTAAACAATTTGTTTCAAAACAGGTACTATTCCTTTCTACTCAAGAATCTAAATTCTGTTTTTTTTATTACTTAAAACTATCATTTTTAAGTTGAAAAAAAAAACATTTAAAAATCAAAATAGTTACCAACTAAAATCTTAATAAATATTTGTTCCTTTAATATCTATACAAAAGTTGGTGAATCGACATTTCTATTACATCGTCAGAAAATCTGTAAAACATAACAAAATAAGCCACAAATTATTACTAATATGTGAATATAATATAAATAATAAACAATTAACCTAACGAAAAAATATAGAATATTTGTTTACGATTGAAATATTATTTTAATTATTTTAAAAAATGTTAATCAAGATCAATATTTTATTTATATTTTCATTAATATTTCTTCAAAGTCGAAACTTCCACATTTTCGTGGATATTGGCATTTTAGACTTTATTTGTCAACTAAATTTTGAAACCAAAGACTTTTTTTTTTTTTTTTTTTTAATGAATAAAACCAAAGATGTAATATCGTAACTAACTAAAATTTTTAAGATAGGGTTAGAGAATACTGGAATAAATTTTGAAAATTATAAAGTAACGGTAGGATATAATAAAATGAGATTATATATAAAAAATATATTTATATATTTCTTTTGAGGAAGATTCTTTTTAGTATAATTGGAGGTAGAGAAATTTGGATCGCAAATTTTATGTTACCAATATGCTCGAATGCTAATTGTACTATGCTCGATTTGACTTTCTAGATTGGGACTTAAAACTGAGTAAAGGGAGTAAAAAACTTATTCAATGATTAATAATTATATATTTCTGGAATATTAACGATGATTTATTTTTGAATAATACGTGCAGGTGGTTTTCTACAAGAGAATCAACCGGGTGTTTTTCATAGATGCAATTCCCAAATCGCCCTCGGGCAAGATCTTGAGGAAGGACTTGAGGGCAAAGTTGGCAGCTGCTTTTCCAAATTCTAATTAATTAATTTCTTTTTTCTCTTTTGCAATTTCACCCCCACACCAAATGATAATGCAAGTTTATTTTATTTTATTTTTATTTTCCCTCCCTTTCTCTCGTTATTACTTTCTTTTTTTAAAAAAGAAAAAGTTGTATTTTATATGGAAAATTGTTGTACTTGTATGAACTACCTAAAGCTCTCTACATCTTTTCATATAAATGAAGAAACTGATTTCCAGTTTCG

mRNA sequence

TTTGAACTCAGAAGAACAATAAAACCTTCAAAATAAAAAAAATAATAATAAAAAAAAAAGTTTTATCTGTTCTTCTTCTTCTTCTTTGGGCATTGAAATGGGCATTGAAACAGTTCAGAATGACGTCATTTTCCGGTCAAAACTACCGGACATCTACATTCCAAAGCACCTTCCTCTGCATTCCTACTGCCTACACGAAAACGCCGCCAAAATCGGCGACCGGACTTGTCTAATCAACGGCGTCACCGGCGAATCCTTCACCTACAACGATGTCGATCTCTCCGCTCGCAAAGTCGCCGCCGGACTAAACAAACTCGGAATTACAAAAAGCGATGTAATTATGCTCCTCCTCCCAAATTCGCCGGAATTCGTCTTCACTTTTCTCGGTGCCTCATACCTCGGCGCAATTATGACGGCGGCGAATCCTTTCTTCACAGCACAAGAAATCGCAAAGCAAGCAAAAGGATCGAAAGCGAAATTGATCCTAACACACTCATCATATTACGAAAAAGTAAAGGAAATAACAGAGGAATTACCTGACGTCAAAATCATGACCGTCGATTCTCCACCGGACGGTTGTTTATGGTTCGGCGATCTAATTAAAGCCGACGAACGAGATATTCCGACGGTAGAGATCGATCCAGACGACGTCGTTGCGCTGCCGTATTCCTCTGGAACTACCGGATTACCGAAAGGAGTGATGCTCACACATAAAAGTTTGGTTACGAGTGTGGCTCAGCAAGTGGATGGTGAAAATCCGAATCTGTATTATCGAAATGAGGATGTGATTCTGTGCGTTTTACCGTTGTTTCATATTTATTCGCTTAATTCGGTTTTGCTCTGTGGATTACGAGCTGGAACTACGATTTTGATTATGCCGAAATTTGAGATCGGTTTGCTTTTACAATTGGTGGAGAAATATAGAGTGTCGATTGCGCCGATTGTCCCACCGATTGTTTTGGCAATTGCGAAGTCGCCGGAGCTTGAGAAGTATGATCTTTCGTCGATCAGAGTGATTAAATGCGGCGGCGCTCCGCTTGGGAAGGAGCTTGAAGATACCGTGAGAGCTAAGTTTCCTAAGGCTGTGCTCGGCCAGGGGTACGGAATGACGGAGGCGGGTCCAGTACTAACAATGGGTTTAGCATTTGCAAAGGAGCCATTTCCGATGAAACCAGGCGCCTGCGGCACCGTCGTCAGAAACGCCGAGATGAAGATCGTCGACACCGAAACCGGCAGCTCATTGCCTCGAAACACGCCGGGAGAGATCTGCATCAGAGGCGATCAAATCATGAAAGGGTATCTCAACAATCCGGAGGCGACGGCGGCCACAATCGACAAGGACGGCTGGTTGCACACCGGCGATATCGGATTCATCGACGACGAAGACGAGCTCTTCATTGTGGATCGGTTGAAGGAACTGATAAAGTACAAGGGATTTCAAGTGGCTCCGGCGGAACTGGAAGCTCTGTTGCTGACTCATCCGGTGATCTCTGATGCCGCCGTCGTACCAATGAAAGATGAACAAGCTGGAGAGGTTCCCGTTGCGTTCGTTGTGAAATTGAAGAATTCTGAAGTAACTGAGGATGAAATTAAACAATTTGTTTCAAAACAGGTGGTTTTCTACAAGAGAATCAACCGGGTGTTTTTCATAGATGCAATTCCCAAATCGCCCTCGGGCAAGATCTTGAGGAAGGACTTGAGGGCAAAGTTGGCAGCTGCTTTTCCAAATTCTAATTAATTAATTTCTTTTTTCTCTTTTGCAATTTCACCCCCACACCAAATGATAATGCAAGTTTATTTTATTTTATTTTTATTTTCCCTCCCTTTCTCTCGTTATTACTTTCTTTTTTTAAAAAAGAAAAAGTTGTATTTTATATGGAAAATTGTTGTACTTGTATGAACTACCTAAAGCTCTCTACATCTTTTCATATAAATGAAGAAACTGATTTCCAGTTTCG

Coding sequence (CDS)

ATGGGCATTGAAACAGTTCAGAATGACGTCATTTTCCGGTCAAAACTACCGGACATCTACATTCCAAAGCACCTTCCTCTGCATTCCTACTGCCTACACGAAAACGCCGCCAAAATCGGCGACCGGACTTGTCTAATCAACGGCGTCACCGGCGAATCCTTCACCTACAACGATGTCGATCTCTCCGCTCGCAAAGTCGCCGCCGGACTAAACAAACTCGGAATTACAAAAAGCGATGTAATTATGCTCCTCCTCCCAAATTCGCCGGAATTCGTCTTCACTTTTCTCGGTGCCTCATACCTCGGCGCAATTATGACGGCGGCGAATCCTTTCTTCACAGCACAAGAAATCGCAAAGCAAGCAAAAGGATCGAAAGCGAAATTGATCCTAACACACTCATCATATTACGAAAAAGTAAAGGAAATAACAGAGGAATTACCTGACGTCAAAATCATGACCGTCGATTCTCCACCGGACGGTTGTTTATGGTTCGGCGATCTAATTAAAGCCGACGAACGAGATATTCCGACGGTAGAGATCGATCCAGACGACGTCGTTGCGCTGCCGTATTCCTCTGGAACTACCGGATTACCGAAAGGAGTGATGCTCACACATAAAAGTTTGGTTACGAGTGTGGCTCAGCAAGTGGATGGTGAAAATCCGAATCTGTATTATCGAAATGAGGATGTGATTCTGTGCGTTTTACCGTTGTTTCATATTTATTCGCTTAATTCGGTTTTGCTCTGTGGATTACGAGCTGGAACTACGATTTTGATTATGCCGAAATTTGAGATCGGTTTGCTTTTACAATTGGTGGAGAAATATAGAGTGTCGATTGCGCCGATTGTCCCACCGATTGTTTTGGCAATTGCGAAGTCGCCGGAGCTTGAGAAGTATGATCTTTCGTCGATCAGAGTGATTAAATGCGGCGGCGCTCCGCTTGGGAAGGAGCTTGAAGATACCGTGAGAGCTAAGTTTCCTAAGGCTGTGCTCGGCCAGGGGTACGGAATGACGGAGGCGGGTCCAGTACTAACAATGGGTTTAGCATTTGCAAAGGAGCCATTTCCGATGAAACCAGGCGCCTGCGGCACCGTCGTCAGAAACGCCGAGATGAAGATCGTCGACACCGAAACCGGCAGCTCATTGCCTCGAAACACGCCGGGAGAGATCTGCATCAGAGGCGATCAAATCATGAAAGGGTATCTCAACAATCCGGAGGCGACGGCGGCCACAATCGACAAGGACGGCTGGTTGCACACCGGCGATATCGGATTCATCGACGACGAAGACGAGCTCTTCATTGTGGATCGGTTGAAGGAACTGATAAAGTACAAGGGATTTCAAGTGGCTCCGGCGGAACTGGAAGCTCTGTTGCTGACTCATCCGGTGATCTCTGATGCCGCCGTCGTACCAATGAAAGATGAACAAGCTGGAGAGGTTCCCGTTGCGTTCGTTGTGAAATTGAAGAATTCTGAAGTAACTGAGGATGAAATTAAACAATTTGTTTCAAAACAGGTGGTTTTCTACAAGAGAATCAACCGGGTGTTTTTCATAGATGCAATTCCCAAATCGCCCTCGGGCAAGATCTTGAGGAAGGACTTGAGGGCAAAGTTGGCAGCTGCTTTTCCAAATTCTAATTAA

Protein sequence

MGIETVQNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVDLSARKVAAGLNKLGITKSDVIMLLLPNSPEFVFTFLGASYLGAIMTAANPFFTAQEIAKQAKGSKAKLILTHSSYYEKVKEITEELPDVKIMTVDSPPDGCLWFGDLIKADERDIPTVEIDPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHIYSLNSVLLCGLRAGTTILIMPKFEIGLLLQLVEKYRVSIAPIVPPIVLAIAKSPELEKYDLSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFIDDEDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKLKNSEVTEDEIKQFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAAAFPNSN
Homology
BLAST of Bhi11G001234 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 771.2 bits (1990), Expect = 5.7e-223
Identity = 382/541 (70.61%), Postives = 456/541 (84.29%), Query Frame = 0

Query: 8   NDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVDLSARKVA 67
           NDVIFRS+LPDIYIP HLPLH Y + EN ++   + CLING TGE +TY DV +++RK+A
Sbjct: 19  NDVIFRSRLPDIYIPNHLPLHDY-IFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 78

Query: 68  AGLNKLGITKSDVIMLLLPNSPEFVFTFLGASYLGAIMTAANPFFTAQEIAKQAKGSKAK 127
           AGL+ LG+ + DV+M+LLPNSPE V TFL AS++GAI T+ANPFFT  EI+KQAK S AK
Sbjct: 79  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 138

Query: 128 LILTHSSYYEKVKEITEELPDVKIMTVDSP--PDGCLWFGDLIKADERDIPTV--EIDPD 187
           LI+T S Y +K+K +  +   V I+T DS   P+ CL F +L +++E  + ++  +I P+
Sbjct: 139 LIVTQSRYVDKIKNLQND--GVLIVTTDSDAIPENCLRFSELTQSEEPRVDSIPEKISPE 198

Query: 188 DVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHIYSL 247
           DVVALP+SSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY+  +DVILCVLP+FHIY+L
Sbjct: 199 DVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYAL 258

Query: 248 NSVLLCGLRAGTTILIMPKFEIGLLLQLVEKYRVSIAPIVPPIVLAIAKSPELEKYDLSS 307
           NS++LC LR G TILIMPKFEI LLL+ +++ +V++A +VPPIVLAIAKSPE EKYDLSS
Sbjct: 259 NSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSS 318

Query: 308 IRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACG 367
           +R++K G APLGKELED + AKFP A LGQGYGMTEAGPVL M L FAKEPFP+K GACG
Sbjct: 319 VRMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACG 378

Query: 368 TVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDI 427
           TVVRNAEMKI+D +TG SLPRN PGEICIRG+QIMKGYLN+P ATA+TIDKDGWLHTGD+
Sbjct: 379 TVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDV 438

Query: 428 GFIDDEDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVA 487
           GFIDD+DELFIVDRLKELIKYKGFQVAPAELE+LL+ HP I+D AVV MK+E AGEVPVA
Sbjct: 439 GFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVA 498

Query: 488 FVVKLKNSEVTEDEIKQFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAAAFP 545
           FVV+ K+S ++EDEIKQFVSKQVVFYKRIN+VFF D+IPK+PSGKILRKDLRA+LA    
Sbjct: 499 FVVRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLANGLM 556

BLAST of Bhi11G001234 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 762.3 bits (1967), Expect = 2.7e-220
Identity = 384/540 (71.11%), Postives = 447/540 (82.78%), Query Frame = 0

Query: 8   NDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVDLSARKVA 67
           +DVIFRSKLPDIYIP HL LH Y + +N ++   + CLING TG  +TY+DV + +R++A
Sbjct: 21  SDVIFRSKLPDIYIPNHLSLHDY-IFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIA 80

Query: 68  AGLNKLGITKSDVIMLLLPNSPEFVFTFLGASYLGAIMTAANPFFTAQEIAKQAKGSKAK 127
           A  +KLG+ ++DV+MLLLPN PEFV +FL AS+ GA  TAANPFFT  EIAKQAK S  K
Sbjct: 81  ANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTK 140

Query: 128 LILTHSSYYEKVKEITEELPDVKIMTVDSP-----PDGCLWFGDLIKAD---ERDIPTVE 187
           LI+T + Y +K+K +  +   V I+ +D       P+GCL F +L ++       I +VE
Sbjct: 141 LIITEARYVDKIKPLQND-DGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVE 200

Query: 188 IDPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYRNEDVILCVLPLFH 247
           I PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY+ ++DVILCVLP+FH
Sbjct: 201 ISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFH 260

Query: 248 IYSLNSVLLCGLRAGTTILIMPKFEIGLLLQLVEKYRVSIAPIVPPIVLAIAKSPELEKY 307
           IY+LNS++LCGLR G  ILIMPKFEI LLL+L+++ +V++AP+VPPIVLAIAKS E EKY
Sbjct: 261 IYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKY 320

Query: 308 DLSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKP 367
           DLSSIRV+K G APLGKELED V AKFP A LGQGYGMTEAGPVL M L FAKEPFP+K 
Sbjct: 321 DLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKS 380

Query: 368 GACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLH 427
           GACGTVVRNAEMKIVD +TG SL RN PGEICIRG QIMKGYLNNP ATA TIDKDGWLH
Sbjct: 381 GACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLH 440

Query: 428 TGDIGFIDDEDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGE 487
           TGDIG IDD+DELFIVDRLKELIKYKGFQVAPAELEALL+ HP I+D AVV MK+E AGE
Sbjct: 441 TGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGE 500

Query: 488 VPVAFVVKLKNSEVTEDEIKQFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKLA 540
           VPVAFVVK K+SE++ED++KQFVSKQVVFYKRIN+VFF ++IPK+PSGKILRKDLRAKLA
Sbjct: 501 VPVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKLA 558

BLAST of Bhi11G001234 vs. TAIR 10
Match: AT1G51680.3 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 705.7 bits (1820), Expect = 2.9e-203
Identity = 356/507 (70.22%), Postives = 415/507 (81.85%), Query Frame = 0

Query: 8   NDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVDLSARKVA 67
           +DVIFRSKLPDIYIP HL LH Y + +N ++   + CLING TG  +TY+DV + +R++A
Sbjct: 21  SDVIFRSKLPDIYIPNHLSLHDY-IFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIA 80

Query: 68  AGLNKLGITKSDVIMLLLPNSPEFVFTFLGASYLGAIMTAANPFFTAQEIAKQAKGSKAK 127
           A  +KLG+ ++DV+MLLLPN PEFV +FL AS+ GA  TAANPFFT  EIAKQAK S  K
Sbjct: 81  ANFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTK 140

Query: 128 LILTHSSYYEKVKEITEELPDVKIMTVDSP-----PDGCLWFGDLIKAD---ERDIPTVE 187
           LI+T + Y +K+K +  +   V I+ +D       P+GCL F +L ++       I +VE
Sbjct: 141 LIITEARYVDKIKPLQND-DGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVE 200

Query: 188 IDPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYRNEDVILCVLPLFH 247
           I PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY+ ++DVILCVLP+FH
Sbjct: 201 ISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFH 260

Query: 248 IYSLNSVLLCGLRAGTTILIMPKFEIGLLLQLVEKYRVSIAPIVPPIVLAIAKSPELEKY 307
           IY+LNS++LCGLR G  ILIMPKFEI LLL+L+++ +V++AP+VPPIVLAIAKS E EKY
Sbjct: 261 IYALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKY 320

Query: 308 DLSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKP 367
           DLSSIRV+K G APLGKELED V AKFP A LGQGYGMTEAGPVL M L FAKEPFP+K 
Sbjct: 321 DLSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKS 380

Query: 368 GACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLH 427
           GACGTVVRNAEMKIVD +TG SL RN PGEICIRG QIMKGYLNNP ATA TIDKDGWLH
Sbjct: 381 GACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLH 440

Query: 428 TGDIGFIDDEDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGE 487
           TGDIG IDD+DELFIVDRLKELIKYKGFQVAPAELEALL+ HP I+D AVV MK+E AGE
Sbjct: 441 TGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGE 500

Query: 488 VPVAFVVKLKNSEVTEDEIKQFVSKQV 507
           VPVAFVVK K+SE++ED++KQFVSKQV
Sbjct: 501 VPVAFVVKSKDSELSEDDVKQFVSKQV 525

BLAST of Bhi11G001234 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 700.7 bits (1807), Expect = 9.5e-202
Identity = 345/531 (64.97%), Postives = 433/531 (81.54%), Query Frame = 0

Query: 11  IFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVDLSARKVAAGL 70
           IFRSKLPDI IP HLPLH+YC  E  + + D+ CLI G TG+S+TY +  L  R+VA+GL
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCF-EKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGL 93

Query: 71  NKLGITKSDVIMLLLPNSPEFVFTFLGASYLGAIMTAANPFFTAQEIAKQAKGSKAKLIL 130
            KLGI K DVIM+LL NS EFVF+F+GAS +GA+ T ANPF+T+QE+ KQ K S AKLI+
Sbjct: 94  YKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLII 153

Query: 131 THSSYYEKVKEITEELPDVKIMTVDSP-PDGCLWFGDLIKADERD--IPTVEIDPDDVVA 190
           THS Y +K+K + E L    ++T D P P+ CL F  LI  DE +    TV+I  DD  A
Sbjct: 154 THSQYVDKLKNLGENL---TLITTDEPTPENCLPFSTLITDDETNPFQETVDIGGDDAAA 213

Query: 191 LPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHIYSLNSVL 250
           LP+SSGTTGLPKGV+LTHKSL+TSVAQQVDG+NPNLY ++ DVILCVLPLFHIYSLNSVL
Sbjct: 214 LPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVL 273

Query: 251 LCGLRAGTTILIMPKFEIGLLLQLVEKYRVSIAPIVPPIVLAIAKSPELEKYDLSSIRVI 310
           L  LR+G T+L+M KFEIG LL L++++RV+IA +VPP+V+A+AK+P +  YDLSS+R +
Sbjct: 274 LNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRFV 333

Query: 311 KCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVR 370
             G APLGKEL+D++R + P+A+LGQGYGMTEAGPVL+M L FAKEP P K G+CGTVVR
Sbjct: 334 LSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVR 393

Query: 371 NAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFID 430
           NAE+K+V  ET  SL  N PGEICIRG QIMK YLN+PEAT+ATID++GWLHTGDIG++D
Sbjct: 394 NAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYVD 453

Query: 431 DEDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVK 490
           ++DE+FIVDRLKE+IK+KGFQV PAELE+LL+ H  I+DAAVVP  DE AGEVPVAFVV+
Sbjct: 454 EDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVR 513

Query: 491 LKNSEVTEDEIKQFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKL 539
              +++TE+++K++V+KQVVFYKR+++VFF+ +IPKSPSGKILRKDL+AKL
Sbjct: 514 SNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of Bhi11G001234 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 672.2 bits (1733), Expect = 3.6e-193
Identity = 333/548 (60.77%), Postives = 421/548 (76.82%), Query Frame = 0

Query: 4   ETVQNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGD----RTCLINGVTGESFTYNDV 63
           E   +D IFRSKLPDI+IP HLPL  Y + +  +  GD     TC+I+G TG   TY DV
Sbjct: 21  EEPSHDFIFRSKLPDIFIPNHLPLTDY-VFQRFSGDGDGDSSTTCIIDGATGRILTYADV 80

Query: 64  DLSARKVAAGLNKLGITKSDVIMLLLPNSPEFVFTFLGASYLGAIMTAANPFFTAQEIAK 123
             + R++AAG+++LGI   DV+MLLLPNSPEF  +FL  +YLGA+ T ANPF+T  EIAK
Sbjct: 81  QTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAK 140

Query: 124 QAKGSKAKLILTHSSYYEKVKEITEELPDVKIMTVD---------SPPDGCLWFGDLIKA 183
           QAK S AK+I+T     +K+  +  +   V I+ +D         S  DGC+ F +L +A
Sbjct: 141 QAKASAAKMIITKKCLVDKLTNLKND--GVLIVCLDDDGDNGVVSSSDDGCVSFTELTQA 200

Query: 184 DERDIPTVEIDPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYRNEDV 243
           DE ++   +I P+D VA+PYSSGTTGLPKGVM+THK LVTS+AQ+VDGENPNL +   DV
Sbjct: 201 DETELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDV 260

Query: 244 ILCVLPLFHIYSLNSVLLCGLRAGTTILIMPKFEIGLLLQLVEKYRVSIAPIVPPIVLAI 303
           ILC LP+FHIY+L++++L  +R G  +LI+P+FE+ L+++L+++Y+V++ P+ PP+VLA 
Sbjct: 261 ILCFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAF 320

Query: 304 AKSPELEKYDLSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAF 363
            KSPE E+YDLSS+R++  G A L KELED VR KFP A+ GQGYGMTE+G V    LAF
Sbjct: 321 IKSPETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLAF 380

Query: 364 AKEPFPMKPGACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAA 423
           AK PF  K GACGTV+RNAEMK+VDTETG SLPRN  GEIC+RG Q+MKGYLN+PEATA 
Sbjct: 381 AKNPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATAR 440

Query: 424 TIDKDGWLHTGDIGFIDDEDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVV 483
           TIDKDGWLHTGDIGF+DD+DE+FIVDRLKELIK+KG+QVAPAELEALL++HP I DAAVV
Sbjct: 441 TIDKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVV 500

Query: 484 PMKDEQAGEVPVAFVVKLKNSEVTEDEIKQFVSKQVVFYKRINRVFFIDAIPKSPSGKIL 539
            MKDE A EVPVAFV + + S++TED++K +V+KQVV YKRI  VFFI+ IPK+ SGKIL
Sbjct: 501 AMKDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKIL 560

BLAST of Bhi11G001234 vs. ExPASy Swiss-Prot
Match: O24146 (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1)

HSP 1 Score: 811.2 bits (2094), Expect = 7.0e-234
Identity = 406/541 (75.05%), Postives = 456/541 (84.29%), Query Frame = 0

Query: 4   ETVQNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVDLSA 63
           +T Q D+IFRSKLPDIYIP HLPLHSYC  EN ++   R CLING   + +TY DV+L++
Sbjct: 4   DTKQVDIIFRSKLPDIYIPNHLPLHSYCF-ENISEFSSRPCLINGANKQIYTYADVELNS 63

Query: 64  RKVAAGLNKLGITKSDVIMLLLPNSPEFVFTFLGASYLGAIMTAANPFFTAQEIAKQAKG 123
           RKVAAGL+K GI   D IM+LLPNSPEFVF F+GASYLGAI T ANP FT  E+ KQAK 
Sbjct: 64  RKVAAGLHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKA 123

Query: 124 SKAKLILTHSSYYEKVKEITEELPDVKIMTVDSPPDGCLWFGDLIKADERDIPTVEIDPD 183
           S AK+I+T + +  KVK+   E  DVKI+ +DS P+GCL F  L +A+E DIP VEI PD
Sbjct: 124 SSAKIIVTQACHVNKVKDYAFE-NDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEIQPD 183

Query: 184 DVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHIYSL 243
           DVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY  +EDV+LCVLPLFHIYSL
Sbjct: 184 DVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIYSL 243

Query: 244 NSVLLCGLRAGTTILIMPKFEIGLLLQLVEKYRVSIAPIVPPIVLAIAKSPELEKYDLSS 303
           NSVLLCGLR G  ILIM KF+I   L+L+++Y+V+I P VPPIVLAIAKSP ++ YDLSS
Sbjct: 244 NSVLLCGLRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYDLSS 303

Query: 304 IRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACG 363
           +R +  G APLGKELEDTVRAKFP A LGQGYGMTEAGPVL M LAFAKEPF +K GACG
Sbjct: 304 VRTVMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACG 363

Query: 364 TVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDI 423
           TVVRNAEMKIVD +TG+SLPRN  GEICIRGDQIMKGYLN+PEATA TIDK+GWL+TGDI
Sbjct: 364 TVVRNAEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTGDI 423

Query: 424 GFIDDEDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVA 483
           G+IDD+DELFIVDRLKELIKYKGFQVAPAELEALLL HP ISDAAVVPMKDEQAGEVPVA
Sbjct: 424 GYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVA 483

Query: 484 FVVKLKNSEVTEDEIKQFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAAAFP 543
           FVV+   S +TEDE+K F+SKQV+FYKRI RVFF+DAIPKSPSGKILRKDLRAKLAA  P
Sbjct: 484 FVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAGLP 542

Query: 544 N 545
           N
Sbjct: 544 N 542

BLAST of Bhi11G001234 vs. ExPASy Swiss-Prot
Match: O24145 (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 806.6 bits (2082), Expect = 1.7e-232
Identity = 398/542 (73.43%), Postives = 454/542 (83.76%), Query Frame = 0

Query: 4   ETVQN-DVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVDLS 63
           ET Q+ D+IFRSKLPDIYIPKHLPLHSYC  EN ++   R CLING   + +TY +V+L+
Sbjct: 8   ETKQSGDLIFRSKLPDIYIPKHLPLHSYCF-ENISEFSSRPCLINGANDQIYTYAEVELT 67

Query: 64  ARKVAAGLNKLGITKSDVIMLLLPNSPEFVFTFLGASYLGAIMTAANPFFTAQEIAKQAK 123
            RKVA GLNKLGI + D IM+LLPNSPEFVF F+GASYLGAI T ANP FT  E+ KQAK
Sbjct: 68  CRKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAK 127

Query: 124 GSKAKLILTHSSYYEKVKEITEELPDVKIMTVDSPPDGCLWFGDLIKADERDIPTVEIDP 183
            S AK+I+T S +  KVK+   E  DVK++ +DS P+GCL F +L ++DE +IP V+I P
Sbjct: 128 ASSAKIIITQSCFVGKVKDYASE-NDVKVICIDSAPEGCLHFSELTQSDEHEIPEVKIQP 187

Query: 184 DDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHIYS 243
           DDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGEN NLY  +EDV++CVLPLFHIYS
Sbjct: 188 DDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYS 247

Query: 244 LNSVLLCGLRAGTTILIMPKFEIGLLLQLVEKYRVSIAPIVPPIVLAIAKSPELEKYDLS 303
           LNS+LLCGLR G  ILIM KF+I   L+L++KY+VSI P VPPIVLAIAKSP ++ YDLS
Sbjct: 248 LNSILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLS 307

Query: 304 SIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGAC 363
           S+R +  G APLGKELED VR KFP A LGQGYGMTEAGPVL M LAFAKEPF +K GAC
Sbjct: 308 SVRTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGAC 367

Query: 364 GTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGD 423
           GTVVRNAEMKIVD +TG SLPRN PGEICIRGDQIMKGYLN+PEAT  TIDK+GWLHTGD
Sbjct: 368 GTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGD 427

Query: 424 IGFIDDEDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPV 483
           IGFID++DELFIVDRLKELIKYKGFQVAPAE+EALLL HP ISDAAVVPMKDEQAGEVPV
Sbjct: 428 IGFIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPV 487

Query: 484 AFVVKLKNSEVTEDEIKQFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAAAF 543
           AFVV+   S +TEDE+K F+SKQV+FYKR+ RVFF++ +PKSPSGKILRKDLRA+LAA  
Sbjct: 488 AFVVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAAGV 547

Query: 544 PN 545
           PN
Sbjct: 548 PN 547

BLAST of Bhi11G001234 vs. ExPASy Swiss-Prot
Match: I3PB37 (4-coumarate:CoA ligase 1 OS=Petunia hybrida OX=4102 GN=4CL1 PE=1 SV=1)

HSP 1 Score: 802.7 bits (2072), Expect = 2.5e-231
Identity = 398/544 (73.16%), Postives = 452/544 (83.09%), Query Frame = 0

Query: 1   MGIETVQNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVD 60
           M  ET Q D+IFRSKLPDIYIPKHLPLHSYC  EN ++   R CLING     +TY DV+
Sbjct: 3   METETNQGDLIFRSKLPDIYIPKHLPLHSYCF-ENISEFSSRPCLINGANNHIYTYADVE 62

Query: 61  LSARKVAAGLNKLGITKSDVIMLLLPNSPEFVFTFLGASYLGAIMTAANPFFTAQEIAKQ 120
           L++RKVAAGLNKLGI + D IM+LLPNSPEFVF F+GASYLGAI T ANP FT  E+ KQ
Sbjct: 63  LTSRKVAAGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQ 122

Query: 121 AKGSKAKLILTHSSYYEKVKEITEELPDVKIMTVDSPPDGCLWFGDLIKADERDIPTVEI 180
           AK S AKLI+T + +  KVK+   +  ++ ++ +DS P+GC+ F +L +ADE DIP V+I
Sbjct: 123 AKASNAKLIITQACFVNKVKDYAFD-NNLNVICIDSAPEGCIHFSELTQADEHDIPDVKI 182

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240
             DDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGEN NLY  +EDV++CVLPLFHI
Sbjct: 183 QSDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHI 242

Query: 241 YSLNSVLLCGLRAGTTILIMPKFEIGLLLQLVEKYRVSIAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLR G  ILIM KF+I    +L+EKY+V+I P VPPIVLAIAKSP ++ YD
Sbjct: 243 YSLNSVLLCGLRVGAAILIMQKFDIVQFCELIEKYKVTIGPFVPPIVLAIAKSPVVDNYD 302

Query: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSS+R +  G APLGKELED VR KFP A LGQGYGMTEAGPVL M LAFAKEPF +K G
Sbjct: 303 LSSVRTVMSGAAPLGKELEDAVRIKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSG 362

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVD +TG SLPRN PGEICIRGDQIMKGYLN+P AT  TIDK+GWLHT
Sbjct: 363 ACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPAATTRTIDKEGWLHT 422

Query: 421 GDIGFIDDEDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIG+ID++DELFIVDRLKELIKYKGFQVAPAELEALLL HP ISDAAVVPMKDEQAGEV
Sbjct: 423 GDIGYIDNDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEV 482

Query: 481 PVAFVVKLKNSEVTEDEIKQFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAA 540
           PVAFVV+   S++TEDE+K FVSKQV+FYKRI RVFF++ +PKSPSGKILRKDLRA+LAA
Sbjct: 483 PVAFVVRSNGSDITEDEVKDFVSKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARLAA 542

Query: 541 AFPN 545
             PN
Sbjct: 543 GVPN 544

BLAST of Bhi11G001234 vs. ExPASy Swiss-Prot
Match: P14913 (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL2 PE=2 SV=1)

HSP 1 Score: 798.9 bits (2062), Expect = 3.6e-230
Identity = 396/534 (74.16%), Postives = 456/534 (85.39%), Query Frame = 0

Query: 7   QNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVDLSARKV 66
           + D+IFRSKLPDIYIPKHLPLH+YC  EN +K+GD++CLING TGE+FTY+ V+L +RKV
Sbjct: 8   KEDLIFRSKLPDIYIPKHLPLHTYCF-ENISKVGDKSCLINGATGETFTYSQVELLSRKV 67

Query: 67  AAGLNKLGITKSDVIMLLLPNSPEFVFTFLGASYLGAIMTAANPFFTAQEIAKQAKGSKA 126
           A+GLNKLGI + D IMLLLPNSPE+ F FLGASY GAI T ANPFFT+ E+ KQ K S A
Sbjct: 68  ASGLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASLA 127

Query: 127 KLILTHSSYYEKVKEITEELPDVKIMTVDSPPDGCLWFGDLIKADERDIPTVEIDPDDVV 186
           KLI+T + Y +KVK+   E  +++I+ +D  P  CL F  L++ADE ++P V ID DDVV
Sbjct: 128 KLIITQACYVDKVKDYAAE-KNIQIICIDDAPQDCLHFSKLMEADESEMPEVVIDSDDVV 187

Query: 187 ALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHIYSLNSV 246
           ALPYSSGTTGLPKGVMLTHK LVTSVAQQVDG+NPNLY  +EDV++C+LPLFHIYSLN+V
Sbjct: 188 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAV 247

Query: 247 LLCGLRAGTTILIMPKFEIGLLLQLVEKYRVSIAPIVPPIVLAIAKSPELEKYDLSSIRV 306
           L CGLRAG TILIM KF+I   L+L++KY+V+I P VPPIVLAIAKSP ++KYDLSS+R 
Sbjct: 248 LCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVRT 307

Query: 307 IKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVV 366
           +  G APLGKELED VRAKFP A LGQGYGMTEAGPVL M LAFAKEP+ +K GACGTVV
Sbjct: 308 VMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVV 367

Query: 367 RNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHTGDIGFI 426
           RNAEMKIVD ET +SLPRN  GEICIRGDQIMKGYLN+PE+T  TID++GWLHTGDIGFI
Sbjct: 368 RNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFI 427

Query: 427 DDEDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVV 486
           DD+DELFIVDRLKE+IKYKGFQVAPAELEALLLTHP ISDAAVVPM DE+AGEVPVAFVV
Sbjct: 428 DDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVV 487

Query: 487 KLKNSEVTEDEIKQFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAA 541
           +      TE+EIKQFVSKQVVFYKRI RVFF+DAIPKSPSGKILRKDLRAK+A+
Sbjct: 488 RTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRAKIAS 539

BLAST of Bhi11G001234 vs. ExPASy Swiss-Prot
Match: O24540 (4-coumarate--CoA ligase OS=Vanilla planifolia OX=51239 GN=4CL PE=3 SV=1)

HSP 1 Score: 798.5 bits (2061), Expect = 4.7e-230
Identity = 405/546 (74.18%), Postives = 457/546 (83.70%), Query Frame = 0

Query: 1   MGIETVQNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVD 60
           + IE  + D+IFRSKLPDIYIPK+LPLHSYC  EN +K   R CLING T E FTY DV+
Sbjct: 5   VAIEEQKKDIIFRSKLPDIYIPKNLPLHSYCF-ENISKFSSRPCLINGATDEIFTYADVE 64

Query: 61  LSARKVAAGLNKLGITKSDVIMLLLPNSPEFVFTFLGASYLGAIMTAANPFFTAQEIAKQ 120
           L +R+V +GL+KLGI + D IM+LLPNSPEFVF FLGAS++G+I T ANPFFT+ E+ KQ
Sbjct: 65  LISRRVGSGLSKLGIKQGDTIMILLPNSPEFVFAFLGASFIGSISTMANPFFTSTEVIKQ 124

Query: 121 AKGSKAKLILTHSSYYEKVKEITEELPDVKIMTVDSPPD-----GCLWFGDLIKADERDI 180
           AK S AKLI+T   Y +KVK+   E   VKI+++D+          L F +L  ADE ++
Sbjct: 125 AKASNAKLIITQGCYVDKVKDYACE-NGVKIISIDTTTTADDAANILHFSELTGADENEM 184

Query: 181 PTVEIDPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYRNEDVILCVL 240
           P VEI PD VVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLY  ++DV+LCVL
Sbjct: 185 PKVEISPDGVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYMHSDDVLLCVL 244

Query: 241 PLFHIYSLNSVLLCGLRAGTTILIMPKFEIGLLLQLVEKYRVSIAPIVPPIVLAIAKSPE 300
           PLFHIYSLNSVLLCGLRAG+ ILIM KFEI   L+L++KY+V+I P VPPIVLAIAKS  
Sbjct: 245 PLFHIYSLNSVLLCGLRAGSGILIMQKFEIVPFLELIQKYKVTIGPFVPPIVLAIAKSTV 304

Query: 301 LEKYDLSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPF 360
           ++ YDLSS+R +  G APLGKELED VRAKFP A LGQGYGMTEAGPVL M LAFAKEPF
Sbjct: 305 VDNYDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPF 364

Query: 361 PMKPGACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKD 420
            +K GACGTVVRNAEMKIVD ETGSSLPRN PGEICIRGDQIMKGYLN+PEATA TIDK+
Sbjct: 365 DIKSGACGTVVRNAEMKIVDPETGSSLPRNHPGEICIRGDQIMKGYLNDPEATARTIDKE 424

Query: 421 GWLHTGDIGFIDDEDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDE 480
           GWLHTGDIG+IDD+DELFIVDRLKELIKYKGFQVAPAELEALLLTHP ISDAAVVPMKDE
Sbjct: 425 GWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPCISDAAVVPMKDE 484

Query: 481 QAGEVPVAFVVKLKNSEVTEDEIKQFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLR 540
            AGEVPVAFVVK     +TEDEIKQF+SKQV+FYKRINRVFF++AIPK+PSGKILRKDLR
Sbjct: 485 AAGEVPVAFVVKSNGHNITEDEIKQFISKQVIFYKRINRVFFVEAIPKAPSGKILRKDLR 544

Query: 541 AKLAAA 542
           A+LAAA
Sbjct: 545 ARLAAA 548

BLAST of Bhi11G001234 vs. NCBI nr
Match: XP_038904387.1 (4-coumarate--CoA ligase 1-like [Benincasa hispida])

HSP 1 Score: 1083.6 bits (2801), Expect = 0.0e+00
Identity = 546/546 (100.00%), Postives = 546/546 (100.00%), Query Frame = 0

Query: 1   MGIETVQNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVD 60
           MGIETVQNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVD
Sbjct: 1   MGIETVQNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVD 60

Query: 61  LSARKVAAGLNKLGITKSDVIMLLLPNSPEFVFTFLGASYLGAIMTAANPFFTAQEIAKQ 120
           LSARKVAAGLNKLGITKSDVIMLLLPNSPEFVFTFLGASYLGAIMTAANPFFTAQEIAKQ
Sbjct: 61  LSARKVAAGLNKLGITKSDVIMLLLPNSPEFVFTFLGASYLGAIMTAANPFFTAQEIAKQ 120

Query: 121 AKGSKAKLILTHSSYYEKVKEITEELPDVKIMTVDSPPDGCLWFGDLIKADERDIPTVEI 180
           AKGSKAKLILTHSSYYEKVKEITEELPDVKIMTVDSPPDGCLWFGDLIKADERDIPTVEI
Sbjct: 121 AKGSKAKLILTHSSYYEKVKEITEELPDVKIMTVDSPPDGCLWFGDLIKADERDIPTVEI 180

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240
           DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI
Sbjct: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGTTILIMPKFEIGLLLQLVEKYRVSIAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLRAGTTILIMPKFEIGLLLQLVEKYRVSIAPIVPPIVLAIAKSPELEKYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGLLLQLVEKYRVSIAPIVPPIVLAIAKSPELEKYD 300

Query: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDEDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFIDDEDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV
Sbjct: 421 GDIGFIDDEDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 481 PVAFVVKLKNSEVTEDEIKQFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAA 540
           PVAFVVKLKNSEVTEDEIKQFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAA
Sbjct: 481 PVAFVVKLKNSEVTEDEIKQFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAA 540

Query: 541 AFPNSN 547
           AFPNSN
Sbjct: 541 AFPNSN 546

BLAST of Bhi11G001234 vs. NCBI nr
Match: KAA0034948.1 (4-coumarate--CoA ligase 1 [Cucumis melo var. makuwa] >TYK21303.1 4-coumarate--CoA ligase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1029.2 bits (2660), Expect = 1.3e-296
Identity = 516/546 (94.51%), Postives = 533/546 (97.62%), Query Frame = 0

Query: 1   MGIETVQNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVD 60
           MGIETV+NDVIFRSKLPDIYIP HLPLHSYCL ENAAKIG RTCLINGVTGESFT+NDVD
Sbjct: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60

Query: 61  LSARKVAAGLNKLGITKSDVIMLLLPNSPEFVFTFLGASYLGAIMTAANPFFTAQEIAKQ 120
           L+ARKVA+GLNKLGITK DVIMLLLPNSPEFVF FLGAS+LGAIMTAANPFFTA EIAKQ
Sbjct: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLILTHSSYYEKVKEITEELPDVKIMTVDSPPDGCLWFGDLIKADERDIPTVEI 180
           AKGSKAKLI+T SSYYEK+KEITEELP+VKIMTVDSP DGCLWFGDLIKADER+IP VEI
Sbjct: 121 AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI 180

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240
           DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYY NEDVILCVLPLFHI
Sbjct: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGTTILIMPKFEIGLLLQLVEKYRVSIAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLRAG+TILIMPKFEIGLLLQLVEKY V++APIVPPIVLAIAKSPELEKYD
Sbjct: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD 300

Query: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRVIK GGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDEDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFID++DELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV
Sbjct: 421 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 481 PVAFVVKLKNSEVTEDEIKQFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAA 540
           PVAFVVKLKNSE TEDEIKQF+SKQVVFYK+INRVFFIDAIPKSPSGKILRK+LRAKLAA
Sbjct: 481 PVAFVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLAA 540

Query: 541 AFPNSN 547
           AFPNSN
Sbjct: 541 AFPNSN 546

BLAST of Bhi11G001234 vs. NCBI nr
Match: XP_004137646.3 (4-coumarate--CoA ligase 1 [Cucumis sativus] >KAE8650952.1 hypothetical protein Csa_000821 [Cucumis sativus])

HSP 1 Score: 1023.5 bits (2645), Expect = 6.9e-295
Identity = 509/546 (93.22%), Postives = 531/546 (97.25%), Query Frame = 0

Query: 1   MGIETVQNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVD 60
           MGIETV+NDVIFRSKLPDIYIPKHLPLHSYCL ENAAKIG RTCLINGVTGESFTYNDVD
Sbjct: 1   MGIETVENDVIFRSKLPDIYIPKHLPLHSYCLQENAAKIGHRTCLINGVTGESFTYNDVD 60

Query: 61  LSARKVAAGLNKLGITKSDVIMLLLPNSPEFVFTFLGASYLGAIMTAANPFFTAQEIAKQ 120
           L+ RKVA+GLNKLGITK DVIMLLLPNSPEFVF FLGASYLGAIMTAANPFFTA EI KQ
Sbjct: 61  LTTRKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIGKQ 120

Query: 121 AKGSKAKLILTHSSYYEKVKEITEELPDVKIMTVDSPPDGCLWFGDLIKADERDIPTVEI 180
           AKGSK+KLI+T SSYYEKVKEITEELP+VKIMTVDSPPDGCLWFGDLIKADER++P V+I
Sbjct: 121 AKGSKSKLIITQSSYYEKVKEITEELPEVKIMTVDSPPDGCLWFGDLIKADEREVPRVDI 180

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240
           DP+DVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYY +EDVILCVLPLFHI
Sbjct: 181 DPEDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGHEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGTTILIMPKFEIGLLLQLVEKYRVSIAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLRAG+TILIMPKFEIGLLLQL EKY V++APIVPPIVLAIAKSPELEKYD
Sbjct: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLAEKYGVTVAPIVPPIVLAIAKSPELEKYD 300

Query: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIR+IK GGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAK+PFPMKPG
Sbjct: 301 LSSIRIIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKDPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDEDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFIDD+DE+FIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV
Sbjct: 421 GDIGFIDDDDEMFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 481 PVAFVVKLKNSEVTEDEIKQFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAA 540
           PVAFVVKLKNSE +EDEIKQF+SKQVVFYKRINRVFFI+AIPKSPSGKILRK+LRAKLAA
Sbjct: 481 PVAFVVKLKNSEASEDEIKQFISKQVVFYKRINRVFFIEAIPKSPSGKILRKELRAKLAA 540

Query: 541 AFPNSN 547
           AFP SN
Sbjct: 541 AFPTSN 546

BLAST of Bhi11G001234 vs. NCBI nr
Match: XP_022935389.1 (4-coumarate--CoA ligase 1-like [Cucurbita moschata])

HSP 1 Score: 996.9 bits (2576), Expect = 6.9e-287
Identity = 496/546 (90.84%), Postives = 520/546 (95.24%), Query Frame = 0

Query: 1   MGIETVQNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVD 60
           M +E +QND IFRSKLPDIYIP HLPLHSYCLHEN AKIG RTCLIN VTGESFTY+DVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60

Query: 61  LSARKVAAGLNKLGITKSDVIMLLLPNSPEFVFTFLGASYLGAIMTAANPFFTAQEIAKQ 120
           L+ARKVA+GLNKLGI + DVIML+L NSPEFVF FLGASY GAIMTAANPFFTA EIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVIMLILQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLILTHSSYYEKVKEITEELPDVKIMTVDSPPDGCLWFGDLIKADERDIPTVEI 180
           AKGS AKLI+T SSYYEKVKEITEELPDVKIMTVDSPPDGCL F DLI+ADER++PTVEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240
           +PDDVVALPYSSGTTGLPKGVMLTH+ LVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGTTILIMPKFEIGLLLQLVEKYRVSIAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLRAGTTILIMPKFEIG LLQLV+K++VSIAPIVPPIVLAIAKSP+L+KYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTE G SLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDEDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFIDD+DELFIVDRLKELIKYKGFQVAPAE+EALLLTHP ISDAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVKLKNSEVTEDEIKQFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAA 540
           PVAFVV+LKNSEVTEDEIKQF+SKQVVFYKRI R F IDAIPKSPSGKILRK+LRAKLAA
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAA 540

Query: 541 AFPNSN 547
            FPNSN
Sbjct: 541 GFPNSN 546

BLAST of Bhi11G001234 vs. NCBI nr
Match: KAG6581611.1 (4-coumarate--CoA ligase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 996.1 bits (2574), Expect = 1.2e-286
Identity = 496/546 (90.84%), Postives = 519/546 (95.05%), Query Frame = 0

Query: 1   MGIETVQNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVD 60
           M +E +QND IFRSKLPDIYIP HLPLHSYCLHEN AKIG RTCLIN VTGESFTY+DVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60

Query: 61  LSARKVAAGLNKLGITKSDVIMLLLPNSPEFVFTFLGASYLGAIMTAANPFFTAQEIAKQ 120
           L+ARKVA+GLNKLGI + DVIMLLL NSPEFVF FLGASY GAIMTAANPFFTA EIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVIMLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLILTHSSYYEKVKEITEELPDVKIMTVDSPPDGCLWFGDLIKADERDIPTVEI 180
           AKGS AKLI+T SSYYEKVKEITEELPDVKIMTVDSPPDGCL F DLI+ADER++PTVEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240
           +PDDVVALPYSSGTTGLPKGVMLTH+ LVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGTTILIMPKFEIGLLLQLVEKYRVSIAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLRAGTTILIMPKFEIG LLQLV+K++VSIAPIVPPIVLAIAKSP+L+KYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTE G SLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDEDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFIDD+DELFIVDRLKELIKYKGFQVAPAE+EALLLTHP ISDAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVKLKNSEVTEDEIKQFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAA 540
           PVAFVV+LKNSEVTEDEIKQF+SKQVVFYKRI R F IDAIPKSPSGKILRK+LRAKLA 
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 540

Query: 541 AFPNSN 547
            FPNSN
Sbjct: 541 GFPNSN 546

BLAST of Bhi11G001234 vs. ExPASy TrEMBL
Match: A0A5A7SWF8 (4-coumarate--CoA ligase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1199G00110 PE=4 SV=1)

HSP 1 Score: 1029.2 bits (2660), Expect = 6.1e-297
Identity = 516/546 (94.51%), Postives = 533/546 (97.62%), Query Frame = 0

Query: 1   MGIETVQNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVD 60
           MGIETV+NDVIFRSKLPDIYIP HLPLHSYCL ENAAKIG RTCLINGVTGESFT+NDVD
Sbjct: 1   MGIETVENDVIFRSKLPDIYIPNHLPLHSYCLQENAAKIGHRTCLINGVTGESFTFNDVD 60

Query: 61  LSARKVAAGLNKLGITKSDVIMLLLPNSPEFVFTFLGASYLGAIMTAANPFFTAQEIAKQ 120
           L+ARKVA+GLNKLGITK DVIMLLLPNSPEFVF FLGAS+LGAIMTAANPFFTA EIAKQ
Sbjct: 61  LTARKVASGLNKLGITKRDVIMLLLPNSPEFVFAFLGASHLGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLILTHSSYYEKVKEITEELPDVKIMTVDSPPDGCLWFGDLIKADERDIPTVEI 180
           AKGSKAKLI+T SSYYEK+KEITEELP+VKIMTVDSP DGCLWFGDLIKADER+IP VEI
Sbjct: 121 AKGSKAKLIITQSSYYEKLKEITEELPEVKIMTVDSPHDGCLWFGDLIKADEREIPRVEI 180

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240
           DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYY NEDVILCVLPLFHI
Sbjct: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYGNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGTTILIMPKFEIGLLLQLVEKYRVSIAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLRAG+TILIMPKFEIGLLLQLVEKY V++APIVPPIVLAIAKSPELEKYD
Sbjct: 241 YSLNSVLLCGLRAGSTILIMPKFEIGLLLQLVEKYGVTVAPIVPPIVLAIAKSPELEKYD 300

Query: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRVIK GGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDEDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFID++DELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV
Sbjct: 421 GDIGFIDEDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 481 PVAFVVKLKNSEVTEDEIKQFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAA 540
           PVAFVVKLKNSE TEDEIKQF+SKQVVFYK+INRVFFIDAIPKSPSGKILRK+LRAKLAA
Sbjct: 481 PVAFVVKLKNSEATEDEIKQFISKQVVFYKKINRVFFIDAIPKSPSGKILRKELRAKLAA 540

Query: 541 AFPNSN 547
           AFPNSN
Sbjct: 541 AFPNSN 546

BLAST of Bhi11G001234 vs. ExPASy TrEMBL
Match: A0A6J1F599 (4-coumarate--CoA ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111442288 PE=4 SV=1)

HSP 1 Score: 996.9 bits (2576), Expect = 3.3e-287
Identity = 496/546 (90.84%), Postives = 520/546 (95.24%), Query Frame = 0

Query: 1   MGIETVQNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVD 60
           M +E +QND IFRSKLPDIYIP HLPLHSYCLHEN AKIG RTCLIN VTGESFTY+DVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60

Query: 61  LSARKVAAGLNKLGITKSDVIMLLLPNSPEFVFTFLGASYLGAIMTAANPFFTAQEIAKQ 120
           L+ARKVA+GLNKLGI + DVIML+L NSPEFVF FLGASY GAIMTAANPFFTA EIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVIMLILQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLILTHSSYYEKVKEITEELPDVKIMTVDSPPDGCLWFGDLIKADERDIPTVEI 180
           AKGS AKLI+T SSYYEKVKEITEELPDVKIMTVDSPPDGCL F DLI+ADER++PTVEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240
           +PDDVVALPYSSGTTGLPKGVMLTH+ LVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGTTILIMPKFEIGLLLQLVEKYRVSIAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLRAGTTILIMPKFEIG LLQLV+K++VSIAPIVPPIVLAIAKSP+L+KYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTE G SLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDEDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFIDD+DELFIVDRLKELIKYKGFQVAPAE+EALLLTHP ISDAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVKLKNSEVTEDEIKQFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAA 540
           PVAFVV+LKNSEVTEDEIKQF+SKQVVFYKRI R F IDAIPKSPSGKILRK+LRAKLAA
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAA 540

Query: 541 AFPNSN 547
            FPNSN
Sbjct: 541 GFPNSN 546

BLAST of Bhi11G001234 vs. ExPASy TrEMBL
Match: A0A6J1J912 (4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482411 PE=4 SV=1)

HSP 1 Score: 988.4 bits (2554), Expect = 1.2e-284
Identity = 493/546 (90.29%), Postives = 517/546 (94.69%), Query Frame = 0

Query: 1   MGIETVQNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVD 60
           M +E +QND IFRSKLPDIYIP HLPLHSYCLHEN AKIG RTCLIN VT ESFTY+DVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINAVTAESFTYHDVD 60

Query: 61  LSARKVAAGLNKLGITKSDVIMLLLPNSPEFVFTFLGASYLGAIMTAANPFFTAQEIAKQ 120
           L+ARKVA+GLNKLGI + DVI+LLL NSPEFVF FLGASY GAIMTAANPFFTA EIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVILLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLILTHSSYYEKVKEITEELPDVKIMTVDSPPDGCLWFGDLIKADERDIPTVEI 180
           AKGS AKLI+T SSYYEKVKEITEELPDVKIMTVDSPPDGCL F DLI+ADER++PTVEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240
           +PDDVVALPYSSGTTGLPKGVMLTH+ LVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGTTILIMPKFEIGLLLQLVEKYRVSIAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLRAGTTILIMPKFEIG LLQLV+K++VSIAPIVPPIVLAIAKSP+L+KYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIRVIKCGGAPLGKELED+VRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDSVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAEMKIVDTE G SLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGCSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGFIDDEDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIGFIDD+DELFIVDRLKELIKYKGFQVAPAELEALLLTHP ISDAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVKLKNSEVTEDEIKQFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAA 540
           PVAFVV+LKNSEVTEDEIKQF+SKQVVFYKRI R F IDAIPKSPSGKILRK+LRAKLA 
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 540

Query: 541 AFPNSN 547
            FP SN
Sbjct: 541 GFPISN 546

BLAST of Bhi11G001234 vs. ExPASy TrEMBL
Match: A0A6J1KCG9 (4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493060 PE=4 SV=1)

HSP 1 Score: 964.5 bits (2492), Expect = 1.8e-277
Identity = 488/546 (89.38%), Postives = 509/546 (93.22%), Query Frame = 0

Query: 1   MGIETVQNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVD 60
           M  ETV +D+IFRSKLPDIYIPKHLPLHSYCL E  A+IG RTCLIN VTGESFTY+DVD
Sbjct: 1   MANETV-HDIIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHRTCLINAVTGESFTYSDVD 60

Query: 61  LSARKVAAGLNKLGITKSDVIMLLLPNSPEFVFTFLGASYLGAIMTAANPFFTAQEIAKQ 120
           L+ARK A+GL KLGI K DVIMLLLPNSPEFVF FLGASYLGAIMTAANPFFTA EIAKQ
Sbjct: 61  LAARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLILTHSSYYEKVKEITEELPDVKIMTVDSPPDGCLWFGDLIKADERDIPTVEI 180
           AKGSKAKL +T SSYYEKVKEITE+LPD KIMTVDSPP GCL F DLI+  + +IP VEI
Sbjct: 121 AKGSKAKLTITQSSYYEKVKEITEQLPDAKIMTVDSPPLGCLSFADLIQGGDHEIPAVEI 180

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240
            PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLYYRN+DVILCVLPLFHI
Sbjct: 181 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGTTILIMPKFEIGLLLQLVEKYRVSIAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLRAG TILIMPKFEIG LLQLVEKYRVSIAPIVPPIVLAIAKSP+LEKYD
Sbjct: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYD 300

Query: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIR+IK GGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAE+KIVDTE G SLPRNTPGEICI+GDQIMKGYLNNPEATAATID +GWLHT
Sbjct: 361 ACGTVVRNAEIKIVDTENGGSLPRNTPGEICIKGDQIMKGYLNNPEATAATIDNEGWLHT 420

Query: 421 GDIGFIDDEDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIG IDD+DELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV
Sbjct: 421 GDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 481 PVAFVVKLKNSEVTEDEIKQFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAA 540
           PVAFVVKLKNS+VTEDEIKQF+SKQVVFYKRINRVFFI AIPKSPSGKILRK+LRAKLAA
Sbjct: 481 PVAFVVKLKNSKVTEDEIKQFISKQVVFYKRINRVFFIGAIPKSPSGKILRKELRAKLAA 540

Query: 541 AFPNSN 547
            FP  N
Sbjct: 541 DFPIPN 545

BLAST of Bhi11G001234 vs. ExPASy TrEMBL
Match: A0A6J1G9T7 (4-coumarate--CoA ligase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452267 PE=4 SV=1)

HSP 1 Score: 963.0 bits (2488), Expect = 5.3e-277
Identity = 489/543 (90.06%), Postives = 506/543 (93.19%), Query Frame = 0

Query: 1   MGIETVQNDVIFRSKLPDIYIPKHLPLHSYCLHENAAKIGDRTCLINGVTGESFTYNDVD 60
           M  ETV +D IFRSKLPDIYIPKHLPLHSYCL E  A+IG RTCLIN VTGESFTY+DVD
Sbjct: 1   MATETV-HDFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHRTCLINAVTGESFTYSDVD 60

Query: 61  LSARKVAAGLNKLGITKSDVIMLLLPNSPEFVFTFLGASYLGAIMTAANPFFTAQEIAKQ 120
           L+ARK A+GL KLGI K DVIMLLLPNSPEFVF FLGASYLGAIMTAANPFFTA EIAKQ
Sbjct: 61  LAARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLILTHSSYYEKVKEITEELPDVKIMTVDSPPDGCLWFGDLIKADERDIPTVEI 180
           AKGSKAKLI+T SSYYEKVKEITE+L D KIMTVDSP  GCL F DLI+  +  IP VEI
Sbjct: 121 AKGSKAKLIITQSSYYEKVKEITEQLSDAKIMTVDSPSLGCLSFADLIQGGDHKIPAVEI 180

Query: 181 DPDDVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240
            PDDVVALPYSSGTTGLPKGVMLTHK LVTSVAQQVDGENPNLYYRN+DVILCVLPLFHI
Sbjct: 181 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGTTILIMPKFEIGLLLQLVEKYRVSIAPIVPPIVLAIAKSPELEKYD 300
           YSLNSVLLCGLRAG TILIMPKFEIG LLQLVEKYRVSIAPIVPPIVLAIAKSP+LEKYD
Sbjct: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYD 300

Query: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIR+IK GGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEMKIVDTETGSSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420
           ACGTVVRNAE+KIVDTE G SLPRNTPGEICIRGDQIMKGYLNNPEATAATID +GWLHT
Sbjct: 361 ACGTVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDSEGWLHT 420

Query: 421 GDIGFIDDEDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIG IDD+DELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV
Sbjct: 421 GDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480

Query: 481 PVAFVVKLKNSEVTEDEIKQFVSKQVVFYKRINRVFFIDAIPKSPSGKILRKDLRAKLAA 540
           PVAFVVKLKNSEVTEDEIKQF+SKQVVFYKRINRVFFIDAIPKSPSGKILRK+LRAKLAA
Sbjct: 481 PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAA 540

Query: 541 AFP 544
            FP
Sbjct: 541 DFP 542

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT3G21240.15.7e-22370.614-coumarate:CoA ligase 2 [more]
AT1G51680.12.7e-22071.114-coumarate:CoA ligase 1 [more]
AT1G51680.32.9e-20370.224-coumarate:CoA ligase 1 [more]
AT1G65060.19.5e-20264.974-coumarate:CoA ligase 3 [more]
AT3G21230.13.6e-19360.774-coumarate:CoA ligase 5 [more]
Match NameE-valueIdentityDescription
O241467.0e-23475.054-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1[more]
O241451.7e-23273.434-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1[more]
I3PB372.5e-23173.164-coumarate:CoA ligase 1 OS=Petunia hybrida OX=4102 GN=4CL1 PE=1 SV=1[more]
P149133.6e-23074.164-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL2 PE=2 SV=1[more]
O245404.7e-23074.184-coumarate--CoA ligase OS=Vanilla planifolia OX=51239 GN=4CL PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_038904387.10.0e+00100.004-coumarate--CoA ligase 1-like [Benincasa hispida][more]
KAA0034948.11.3e-29694.514-coumarate--CoA ligase 1 [Cucumis melo var. makuwa] >TYK21303.1 4-coumarate--Co... [more]
XP_004137646.36.9e-29593.224-coumarate--CoA ligase 1 [Cucumis sativus] >KAE8650952.1 hypothetical protein C... [more]
XP_022935389.16.9e-28790.844-coumarate--CoA ligase 1-like [Cucurbita moschata][more]
KAG6581611.11.2e-28690.844-coumarate--CoA ligase 1, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A5A7SWF86.1e-29794.514-coumarate--CoA ligase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A6J1F5993.3e-28790.844-coumarate--CoA ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111442288 PE=... [more]
A0A6J1J9121.2e-28490.294-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482411 PE=4 ... [more]
A0A6J1KCG91.8e-27789.384-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493060 PE=4 ... [more]
A0A6J1G9T75.3e-27790.064-coumarate--CoA ligase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 453..528
e-value: 7.0E-17
score: 62.2
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 33..444
e-value: 2.4E-112
score: 375.6
NoneNo IPR availableGENE3D3.30.300.30coord: 439..541
e-value: 1.0E-32
score: 114.9
NoneNo IPR availablePANTHERPTHR24096:SF3824-COUMARATE--COA LIGASE 2-LIKEcoord: 7..540
NoneNo IPR availablePANTHERPTHR24096LONG-CHAIN-FATTY-ACID--COA LIGASEcoord: 7..540
NoneNo IPR availableCDDcd059044CLcoord: 21..534
e-value: 0.0
score: 801.45
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 20..540
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 5..438
e-value: 5.2E-171
score: 570.9
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 188..199

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi11M001234Bhi11M001234mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016874 ligase activity