Bhi11G001154 (gene) Wax gourd (B227) v1

Overview


Name	Bhi11G001154
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	acyl-CoA--sterol O-acyltransferase 1-like
Location	chr11: 39761821 .. 39763265 (+)
RNA-Seq Expression	Bhi11G001154
Synteny	Bhi11G001154

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATATTATAAAAAAAGTGATTTAAAACTATAAATTTTAATTTACCTCCTTCGTCTTCACTTCCATTTCTAGAGAGAGAGACAAAACTAGAGAGAGAAAAAAATGGAGGGAGAAATCATCAACTTGACCAAGGTATGGTTGACCGTCTGTATCTCTCTCTGTTACTGCCATACCGCCGGAAAACTCATCTCTCCCGGCGCCGGCCGCTTCCTCGCCATCGCCCCCGTCGTCGTCCTCTTCTTCCTCCTCCCTCTGCAACTCACCTCCATCCACCTCGGCGGCGCCACTGCCTTCTTCGTCGCTTGGCTCGCAAACTTCAAGCTACTCCTCTTTGCCGCTGGCGACGGCCCTCTCTCCACCACTCCGCCGCTCCCTCTCCGCCGATTCCTAGCCCTATCTTGCTTACCGATCAAGATTCTTCAAAACCCCTCGGAGAAATCGAGCCCTAAAAATCGCTCCGAAATCCTAAACGAAAAACCCAAATCGCTCCTCAACTACTCGATTAAAGCCCTAATCGTCGGTTTGATGGTGAAATTGTACGATTACTCTGAGTTTATTCACCCGAAAGTTCTGTTGTTCATTTATAGTTGGCACATTTACTTTCTCCTTGAGATTATTCTGGCCATTACGCAGTTTTTTGGTCGGAATCTTCTCGGAATCGAGCTAGAGCCGCAGTTTCGCGATCCGTATTACTCGACTTCGCTTCAGGATTTTTGGGGAAGGCGATGGAATCTGATGGCGACGAATATTCTTCAGCCGACGGTTTACAAGCCGACGGTGAAGATCGCAAGTAAGGTAATCGGGCGGATGTGGGCGCCATTGCCGGCGGTGATGGCAACGTTTTTGGTATCGGCAATGATGCACGAACTAATATTTTACTATTTAGGGCGAATGAAGCCAAACTGGGAGCCCACGTGGTTCTTTGTTATCCATGGAATTGCTTTGACGGTGGAGATTCTGGTGAAGAAGATGGTGGGTGAGAAGTTTCGGTTACCGGCGGCGGTGTCTGTGCCGGTGACTCTGGTTTTGATCTTCTCGACGAAAATTTGGTTGTTTTTTCTGTAGTTCATGAGGTTGAAGAGTACGCGGCGGTGGTCAGTTTTGTGAAGAAAGTCGCTTTCTCATTGGTCGGTTGAGTTTTTTATTATTATTTTTCTTTTCATTTTTTTTTTAATTGGTCAATTCGGGTTGGGTGTTGTATTAATTAGATAATTACATTAAGAACTTTTTGTTTCTCTTTAAGAGGTAAATTTCACGAGAATAAACTTAGGGTAATGTATGTTTATATTAAAAAGGCTTTTATCCCCATTTTGCCCCTCATTTTACCTTCCATACACGCATCTCTTTCTCCAACACATCTTTTCAATAAGAATCTCTTTTTGTACAATGTAACTATCACATTTTAAAACAAATCTTCCTCTTATATAAAGAAAACATACAATGAAAC

mRNA sequence

ATATTATAAAAAAAGTGATTTAAAACTATAAATTTTAATTTACCTCCTTCGTCTTCACTTCCATTTCTAGAGAGAGAGACAAAACTAGAGAGAGAAAAAAATGGAGGGAGAAATCATCAACTTGACCAAGGTATGGTTGACCGTCTGTATCTCTCTCTGTTACTGCCATACCGCCGGAAAACTCATCTCTCCCGGCGCCGGCCGCTTCCTCGCCATCGCCCCCGTCGTCGTCCTCTTCTTCCTCCTCCCTCTGCAACTCACCTCCATCCACCTCGGCGGCGCCACTGCCTTCTTCGTCGCTTGGCTCGCAAACTTCAAGCTACTCCTCTTTGCCGCTGGCGACGGCCCTCTCTCCACCACTCCGCCGCTCCCTCTCCGCCGATTCCTAGCCCTATCTTGCTTACCGATCAAGATTCTTCAAAACCCCTCGGAGAAATCGAGCCCTAAAAATCGCTCCGAAATCCTAAACGAAAAACCCAAATCGCTCCTCAACTACTCGATTAAAGCCCTAATCGTCGGTTTGATGGTGAAATTGTACGATTACTCTGAGTTTATTCACCCGAAAGTTCTGTTGTTCATTTATAGTTGGCACATTTACTTTCTCCTTGAGATTATTCTGGCCATTACGCAGTTTTTTGGTCGGAATCTTCTCGGAATCGAGCTAGAGCCGCAGTTTCGCGATCCGTATTACTCGACTTCGCTTCAGGATTTTTGGGGAAGGCGATGGAATCTGATGGCGACGAATATTCTTCAGCCGACGGTTTACAAGCCGACGGTGAAGATCGCAAGTAAGGTAATCGGGCGGATGTGGGCGCCATTGCCGGCGGTGATGGCAACGTTTTTGGTATCGGCAATGATGCACGAACTAATATTTTACTATTTAGGGCGAATGAAGCCAAACTGGGAGCCCACGTGGTTCTTTGTTATCCATGGAATTGCTTTGACGGTGGAGATTCTGGTGAAGAAGATGGTGGGTGAGAAGTTTCGGTTACCGGCGGCGGTGTCTGTGCCGGTGACTCTGGTTTTGATCTTCTCGACGAAAATTTGGTTGTTTTTTCTGTAGTTCATGAGGTTGAAGAGTACGCGGCGGTGGTCAGTTTTGTGAAGAAAGTCGCTTTCTCATTGGTCGGTTGAGTTTTTTATTATTATTTTTCTTTTCATTTTTTTTTTAATTGGTCAATTCGGGTTGGGTGTTGTATTAATTAGATAATTACATTAAGAACTTTTTGTTTCTCTTTAAGAGGTAAATTTCACGAGAATAAACTTAGGGTAATGTATGTTTATATTAAAAAGGCTTTTATCCCCATTTTGCCCCTCATTTTACCTTCCATACACGCATCTCTTTCTCCAACACATCTTTTCAATAAGAATCTCTTTTTGTACAATGTAACTATCACATTTTAAAACAAATCTTCCTCTTATATAAAGAAAACATACAATGAAAC

Coding sequence (CDS)

ATGGAGGGAGAAATCATCAACTTGACCAAGGTATGGTTGACCGTCTGTATCTCTCTCTGTTACTGCCATACCGCCGGAAAACTCATCTCTCCCGGCGCCGGCCGCTTCCTCGCCATCGCCCCCGTCGTCGTCCTCTTCTTCCTCCTCCCTCTGCAACTCACCTCCATCCACCTCGGCGGCGCCACTGCCTTCTTCGTCGCTTGGCTCGCAAACTTCAAGCTACTCCTCTTTGCCGCTGGCGACGGCCCTCTCTCCACCACTCCGCCGCTCCCTCTCCGCCGATTCCTAGCCCTATCTTGCTTACCGATCAAGATTCTTCAAAACCCCTCGGAGAAATCGAGCCCTAAAAATCGCTCCGAAATCCTAAACGAAAAACCCAAATCGCTCCTCAACTACTCGATTAAAGCCCTAATCGTCGGTTTGATGGTGAAATTGTACGATTACTCTGAGTTTATTCACCCGAAAGTTCTGTTGTTCATTTATAGTTGGCACATTTACTTTCTCCTTGAGATTATTCTGGCCATTACGCAGTTTTTTGGTCGGAATCTTCTCGGAATCGAGCTAGAGCCGCAGTTTCGCGATCCGTATTACTCGACTTCGCTTCAGGATTTTTGGGGAAGGCGATGGAATCTGATGGCGACGAATATTCTTCAGCCGACGGTTTACAAGCCGACGGTGAAGATCGCAAGTAAGGTAATCGGGCGGATGTGGGCGCCATTGCCGGCGGTGATGGCAACGTTTTTGGTATCGGCAATGATGCACGAACTAATATTTTACTATTTAGGGCGAATGAAGCCAAACTGGGAGCCCACGTGGTTCTTTGTTATCCATGGAATTGCTTTGACGGTGGAGATTCTGGTGAAGAAGATGGTGGGTGAGAAGTTTCGGTTACCGGCGGCGGTGTCTGTGCCGGTGACTCTGGTTTTGATCTTCTCGACGAAAATTTGGTTGTTTTTTCTGTAG

Protein sequence

MEGEIINLTKVWLTVCISLCYCHTAGKLISPGAGRFLAIAPVVVLFFLLPLQLTSIHLGGATAFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKILQNPSEKSSPKNRSEILNEKPKSLLNYSIKALIVGLMVKLYDYSEFIHPKVLLFIYSWHIYFLLEIILAITQFFGRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVIGRMWAPLPAVMATFLVSAMMHELIFYYLGRMKPNWEPTWFFVIHGIALTVEILVKKMVGEKFRLPAAVSVPVTLVLIFSTKIWLFFL

Homology

[Close]

BLAST of Bhi11G001154 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 288.9 bits (738), Expect = 5.0e-78
Identity = 157/310 (50.65%), Postives = 211/310 (68.06%), Query Frame = 0


Query: 10  KVWLTVCISLCYCHTAGKLISPGAGRFLAIAPVVVLFFLLPLQLTSIHLGGATAFFVAWL 69
           K W  V ISLCY     KL+  G  R +   PV ++FF++P  + S+HL G TAFF+AWL
Sbjct: 6   KAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFIAWL 65

Query: 70  ANFKLLLFAAGDGPLSTT-PPLPLRRFLALSCLPIKILQNPSEKSSPKNRSEILNEKPKS 129
           ANFKLLLFA G GPLS+   PL L  FLA+SCLPIKI  +P  K +  +  E   E P  
Sbjct: 66  ANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSP--KPTKTHSHEGSTEGP-- 125

Query: 130 LLNYSIKALIVGLMVKLYDYSEFIHPKVLLFIYSWHIYFLLEIILAITQFFGRNLLGIEL 189
            L Y+IKA+ V L++K Y+YS  +  KV+L +Y+ HIYF LEIILA T    R +  +EL
Sbjct: 126 -LIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLEL 185

Query: 190 EPQFRDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVIGRMWAPLPAVMATFL 249
           EPQF  PY +TSLQDFWGRRWNLM T IL+PTVY+P++++ S V+G  ++ + A   TF+
Sbjct: 186 EPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VLGPNYSQILAAFGTFV 245

Query: 250 VSAMMHELIFYYLGRMKPNWEPTWFFVIHGIALTVEILVKKMVGEKFRLPAAVSVPVTLV 309
           VS +MHELIF+Y+GR++P+W+  WFF+I+G   TVEI +KK +  ++R P A+S  +TL 
Sbjct: 246 VSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTLT 305

Query: 310 LIFSTKIWLF 319
            +  T +WLF
Sbjct: 306 FVMVTALWLF 309

[Close]

BLAST of Bhi11G001154 vs. TAIR 10
Match: AT5G55340.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 250.0 bits (637), Expect = 2.6e-66
Identity = 139/320 (43.44%), Postives = 194/320 (60.62%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLCYCHTAGKLISPGAGRFLAIAPVVVLFFLLPLQLTSIHLGG 60
           M+ E+ NL KVW++  IS+ YC+     I  GA RFL+++PV+ LF +LPL  +S+HL  
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  ATAFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKILQNPSEKSSPKNRSE 120
            TAFF+ WLANFKL+LF+   GPL    P    RF   +C PIK+ QNP  +        
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLIPI-PTNFPRFFCFTCFPIKVQQNPKSQ-------- 120

Query: 121 ILNEKPKSLLNYSIKALIVGLMVKLYDYSEFIHPKVLLFIYSWHIYFLLEIILAITQFFG 180
             N  PK  L ++IK  I  +++ LY Y + + P +LL +Y  H+Y  +EIIL   +   
Sbjct: 121 --NHLPK--LVFAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVV 180

Query: 181 RNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVIGRMWAPL 240
              LG +LEPQ   PY +TSLQDFWGRRWNLM   IL+P VY P  +++ + +   WA  
Sbjct: 181 FISLGCDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALF 240

Query: 241 PAVMATFLVSAMMHELIFYYLGRMKPNWEPTWFFVIHGIALTVEILVKK--MVGEKFRLP 300
           P ++A F+VS ++HEL+F+YL R  P  E T FFV+HG+   VE+ VKK   V +++RL 
Sbjct: 241 PGILAAFIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRLS 300

Query: 301 AAVSVPVTLVLIFSTKIWLF 319
             VS  +T+  +F T  WLF
Sbjct: 301 PGVSRVLTVGFVFVTGGWLF 307

[Close]

BLAST of Bhi11G001154 vs. TAIR 10
Match: AT5G55380.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 240.4 bits (612), Expect = 2.1e-63
Identity = 134/319 (42.01%), Postives = 192/319 (60.19%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLCYCHTAGKLISPGAGRFLAIAPVVVLFFLLPLQLTSIHLGG 60
           ME +  NL +VW++  ISL YC+     +S G  R L+I PV +LF +LPL L+ +H   
Sbjct: 1   MEEKFRNLIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCA 60

Query: 61  ATAFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKILQNPSEKSSPKNRSE 120
            +  F++WLANFKLLLFA  +GPL   PP  L RF+  +CLPIKI Q+PS  + P     
Sbjct: 61  ISVLFLSWLANFKLLLFAFDEGPLFPLPP-KLSRFICFACLPIKIRQDPSPNAIPN---- 120

Query: 121 ILNEKPKSLLNYSIKALIVGLMVKLYDYSEFIHPKVLLFIYSWHIYFLLEIILAITQFFG 180
            L+ KP      ++K L++G+++ +Y+Y + +   V+L +Y  HIY  +E++L       
Sbjct: 121 -LHPKPMPKWVLAVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAVV 180

Query: 181 RNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVIGRMWAPL 240
             LLG  +EP F +PY +TSLQDFW RRWNLM + +L+ TV+ P  +   +++    A  
Sbjct: 181 STLLGCNIEPVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMF 240

Query: 241 PAVMATFLVSAMMHELIFYYLGRMKPNWEPTWFFVIHGIALTVEILVKKMVGEKFRLP-A 300
             VMA+F VS +MHEL+++Y+ R  P WE T FFV+HG A   EI VK+   +  R P  
Sbjct: 241 AGVMASFFVSGLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIAVKRT--QWLRPPHR 300

Query: 301 AVSVPVTLVLIFSTKIWLF 319
           AVS  V L  +  T +WLF
Sbjct: 301 AVSGLVVLTFVSVTGVWLF 311

[Close]

BLAST of Bhi11G001154 vs. TAIR 10
Match: AT5G55350.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 238.8 bits (608), Expect = 6.0e-63
Identity = 134/320 (41.88%), Postives = 191/320 (59.69%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLCYCHTAGKLISPGAGRFLAIAPVVVLFFLLPLQLTSIHLGG 60
           ME E+++L KVW+   IS+ YC+     I  G  R L++ PV VLF +LPL ++S+H  G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  ATAFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKILQNPSEKSSPKNRSE 120
           +TAFF++WLANFKL+LF+   GPL   P   L RF+  +C PIK+ QNP  +        
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLFPVPS-NLSRFVCFTCFPIKLQQNPKPQ-------- 120

Query: 121 ILNEKPKSLLNYSIKALIVGLMVKLYDYSEFIHPKVLLFIYSWHIYFLLEIILAITQFFG 180
             N+ PK    +++K    G+++ +Y+Y + + P VLL +YS HIY   EI+LA  +   
Sbjct: 121 --NQMPK--WGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLL 180

Query: 181 RNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVIGRMWAPL 240
              L  +LEP F +PY STSLQDFWGRRWNLM   IL+P VY P  ++A + +    A  
Sbjct: 181 SISLWCDLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALF 240

Query: 241 PAVMATFLVSAMMHELIFYYLGRMKPNWEPTWFFVIHGIALTVEILVKK-MVGEKFRLPA 300
             V A+FLVS ++HELIF+Y  R  P  E T FFV+HG+    E   K+  +  ++++  
Sbjct: 241 LGVFASFLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQ 300

Query: 301 AVSVPVTLVLIFSTKIWLFF 320
            VS  +T+  +  T  WLFF
Sbjct: 301 MVSRLLTVGFVVMTGGWLFF 307

[Close]

BLAST of Bhi11G001154 vs. TAIR 10
Match: AT5G55360.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 230.7 bits (587), Expect = 1.6e-60
Identity = 132/323 (40.87%), Postives = 189/323 (58.51%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLCYCHTAGKLISPGAGRFLAIAPVVVLFFLLPLQLTSIHLGG 60
           ME E+ N  K+W++  IS+ YC+     I  G  R L++ PV  LF + PL  + +H  G
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 61  ATAFFVAWLANFKLLLFAAGDGPLSTTPPLP--LRRFLALSCLPIKILQNPSEKSSPKNR 120
             AFF++WLANFKL+LF+   GPLS   PLP  L RF+ ++C PIK  QNP+        
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPLS---PLPRTLSRFICITCFPIKPQQNPN-------- 120

Query: 121 SEILNEKPKSLLNYSIKALIVGLMVKLYDYSEFIHPKVLLFIYSWHIYFLLEIILAITQF 180
             I N K    L ++IK +I  +++++Y+Y +++ P +LL   S HI+  LEI+  + + 
Sbjct: 121 --IQNYKIPIWL-FAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKA 180

Query: 181 FGRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVIGRMWA 240
                LG +LEPQ  +PY +TSLQDFWGRRWNLM   IL+P VY P  ++A + +    A
Sbjct: 181 LVFITLGCDLEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQA 240

Query: 241 PLPAVMATFLVSAMMHELIFYYLGRMKPNWEPTWFFVIHGIALTVEILVKK--MVGEKFR 300
               V A FLVS  +HE++F+YL R  P  E TWFF++HG+    E+ VKK   V   +R
Sbjct: 241 MFLGVFAAFLVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRRWWR 300

Query: 301 LPAAVSVPVTLVLIFSTKIWLFF 320
           +   VS  +T+  +  T  W FF
Sbjct: 301 VSPTVSRLLTVGFVVVTSGWFFF 309

[Close]

BLAST of Bhi11G001154 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 7.1e-77
Identity = 157/310 (50.65%), Postives = 211/310 (68.06%), Query Frame = 0


Query: 10  KVWLTVCISLCYCHTAGKLISPGAGRFLAIAPVVVLFFLLPLQLTSIHLGGATAFFVAWL 69
           K W  V ISLCY     KL+  G  R +   PV ++FF++P  + S+HL G TAFF+AWL
Sbjct: 6   KAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFIAWL 65

Query: 70  ANFKLLLFAAGDGPLSTT-PPLPLRRFLALSCLPIKILQNPSEKSSPKNRSEILNEKPKS 129
           ANFKLLLFA G GPLS+   PL L  FLA+SCLPIKI  +P  K +  +  E   E P  
Sbjct: 66  ANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSP--KPTKTHSHEGSTEGP-- 125

Query: 130 LLNYSIKALIVGLMVKLYDYSEFIHPKVLLFIYSWHIYFLLEIILAITQFFGRNLLGIEL 189
            L Y+IKA+ V L++K Y+YS  +  KV+L +Y+ HIYF LEIILA T    R +  +EL
Sbjct: 126 -LIYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLEL 185

Query: 190 EPQFRDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVIGRMWAPLPAVMATFL 249
           EPQF  PY +TSLQDFWGRRWNLM T IL+PTVY+P++++ S V+G  ++ + A   TF+
Sbjct: 186 EPQFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VLGPNYSQILAAFGTFV 245

Query: 250 VSAMMHELIFYYLGRMKPNWEPTWFFVIHGIALTVEILVKKMVGEKFRLPAAVSVPVTLV 309
           VS +MHELIF+Y+GR++P+W+  WFF+I+G   TVEI +KK +  ++R P A+S  +TL 
Sbjct: 246 VSGIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTINGRWRFPKAISQVLTLT 305

Query: 310 LIFSTKIWLF 319
            +  T +WLF
Sbjct: 306 FVMVTALWLF 309

[Close]

BLAST of Bhi11G001154 vs. ExPASy Swiss-Prot
Match: Q9FJ76 (Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=AT5 PE=2 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 3.7e-65
Identity = 139/320 (43.44%), Postives = 194/320 (60.62%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLCYCHTAGKLISPGAGRFLAIAPVVVLFFLLPLQLTSIHLGG 60
           M+ E+ NL KVW++  IS+ YC+     I  GA RFL+++PV+ LF +LPL  +S+HL  
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 61  ATAFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKILQNPSEKSSPKNRSE 120
            TAFF+ WLANFKL+LF+   GPL    P    RF   +C PIK+ QNP  +        
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLIPI-PTNFPRFFCFTCFPIKVQQNPKSQ-------- 120

Query: 121 ILNEKPKSLLNYSIKALIVGLMVKLYDYSEFIHPKVLLFIYSWHIYFLLEIILAITQFFG 180
             N  PK  L ++IK  I  +++ LY Y + + P +LL +Y  H+Y  +EIIL   +   
Sbjct: 121 --NHLPK--LVFAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVV 180

Query: 181 RNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVIGRMWAPL 240
              LG +LEPQ   PY +TSLQDFWGRRWNLM   IL+P VY P  +++ + +   WA  
Sbjct: 181 FISLGCDLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALF 240

Query: 241 PAVMATFLVSAMMHELIFYYLGRMKPNWEPTWFFVIHGIALTVEILVKK--MVGEKFRLP 300
           P ++A F+VS ++HEL+F+YL R  P  E T FFV+HG+   VE+ VKK   V +++RL 
Sbjct: 241 PGILAAFIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQRWRLS 300

Query: 301 AAVSVPVTLVLIFSTKIWLF 319
             VS  +T+  +F T  WLF
Sbjct: 301 PGVSRVLTVGFVFVTGGWLF 307

[Close]

BLAST of Bhi11G001154 vs. ExPASy Swiss-Prot
Match: Q9FJ72 (Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=AT1 PE=2 SV=1)

HSP 1 Score: 240.4 bits (612), Expect = 2.9e-62
Identity = 134/319 (42.01%), Postives = 192/319 (60.19%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLCYCHTAGKLISPGAGRFLAIAPVVVLFFLLPLQLTSIHLGG 60
           ME +  NL +VW++  ISL YC+     +S G  R L+I PV +LF +LPL L+ +H   
Sbjct: 1   MEEKFRNLIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCA 60

Query: 61  ATAFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKILQNPSEKSSPKNRSE 120
            +  F++WLANFKLLLFA  +GPL   PP  L RF+  +CLPIKI Q+PS  + P     
Sbjct: 61  ISVLFLSWLANFKLLLFAFDEGPLFPLPP-KLSRFICFACLPIKIRQDPSPNAIPN---- 120

Query: 121 ILNEKPKSLLNYSIKALIVGLMVKLYDYSEFIHPKVLLFIYSWHIYFLLEIILAITQFFG 180
            L+ KP      ++K L++G+++ +Y+Y + +   V+L +Y  HIY  +E++L       
Sbjct: 121 -LHPKPMPKWVLAVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAVV 180

Query: 181 RNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVIGRMWAPL 240
             LLG  +EP F +PY +TSLQDFW RRWNLM + +L+ TV+ P  +   +++    A  
Sbjct: 181 STLLGCNIEPVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMF 240

Query: 241 PAVMATFLVSAMMHELIFYYLGRMKPNWEPTWFFVIHGIALTVEILVKKMVGEKFRLP-A 300
             VMA+F VS +MHEL+++Y+ R  P WE T FFV+HG A   EI VK+   +  R P  
Sbjct: 241 AGVMASFFVSGLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIAVKRT--QWLRPPHR 300

Query: 301 AVSVPVTLVLIFSTKIWLF 319
           AVS  V L  +  T +WLF
Sbjct: 301 AVSGLVVLTFVSVTGVWLF 311

[Close]

BLAST of Bhi11G001154 vs. ExPASy Swiss-Prot
Match: Q9FJ75 (Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=AT4 PE=2 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 8.4e-62
Identity = 134/320 (41.88%), Postives = 191/320 (59.69%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLCYCHTAGKLISPGAGRFLAIAPVVVLFFLLPLQLTSIHLGG 60
           ME E+++L KVW+   IS+ YC+     I  G  R L++ PV VLF +LPL ++S+H  G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 61  ATAFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKILQNPSEKSSPKNRSE 120
           +TAFF++WLANFKL+LF+   GPL   P   L RF+  +C PIK+ QNP  +        
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLFPVPS-NLSRFVCFTCFPIKLQQNPKPQ-------- 120

Query: 121 ILNEKPKSLLNYSIKALIVGLMVKLYDYSEFIHPKVLLFIYSWHIYFLLEIILAITQFFG 180
             N+ PK    +++K    G+++ +Y+Y + + P VLL +YS HIY   EI+LA  +   
Sbjct: 121 --NQMPK--WGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLL 180

Query: 181 RNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVIGRMWAPL 240
              L  +LEP F +PY STSLQDFWGRRWNLM   IL+P VY P  ++A + +    A  
Sbjct: 181 SISLWCDLEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALF 240

Query: 241 PAVMATFLVSAMMHELIFYYLGRMKPNWEPTWFFVIHGIALTVEILVKK-MVGEKFRLPA 300
             V A+FLVS ++HELIF+Y  R  P  E T FFV+HG+    E   K+  +  ++++  
Sbjct: 241 LGVFASFLVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVRRWKVSQ 300

Query: 301 AVSVPVTLVLIFSTKIWLFF 320
            VS  +T+  +  T  WLFF
Sbjct: 301 MVSRLLTVGFVVMTGGWLFF 307

[Close]

BLAST of Bhi11G001154 vs. ExPASy Swiss-Prot
Match: Q9XGY6 (Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 3.2e-61
Identity = 132/320 (41.25%), Postives = 195/320 (60.94%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLCYCHTAGKLISP--GAGRFLAIAPVVVLFFLLPLQLTSIHL 60
           +E E+   ++VW++   + CYC      ++P  GA R L + PVV+LF  LPL+L+S HL
Sbjct: 3   VEKELKTFSEVWISAIAAACYCRFV-PAVAPHGGALRLLLLLPVVLLFIFLPLRLSSFHL 62

Query: 61  GGATAFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKILQNPSEKSSPKNR 120
           GG TA ++ WLANFKLLLFA   GPLS  P L L  F++ + LPIK   +PS       R
Sbjct: 63  GGPTALYLVWLANFKLLLFAFHLGPLS-NPSLSLLHFISTTLLPIKFRDDPSNDHEKNKR 122

Query: 121 SEILNEKPKSLLNYSIKALIVGLMVKLYDYSEFIHPKVLLFIYSWHIYFLLEIILAITQF 180
           +  L+ + + ++ +  K +    ++K+Y++ + +   V+  +Y +H Y   EI LA +  
Sbjct: 123 T--LSFEWRKVVLFVAKLVFFAGILKIYEFRKDLPHFVISVLYCFHFYLGTEITLAASAV 182

Query: 181 FGRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVIGRMWA 240
             R  LG++L PQF +PY +TSLQDFWGRRWNLM ++IL  T Y+P  ++ S+ +   W 
Sbjct: 183 IARATLGLDLYPQFNEPYLATSLQDFWGRRWNLMVSDILGLTTYQPVRRVLSRWVRLRWE 242

Query: 241 PLPAVMATFLVSAMMHELIFYYLGRMKPNWEPTWFFVIHGIALTVEILVKKMVGEKFRLP 300
              A++  F VS +MHE+ F+YL R +P+WE T FFV+HG+   VE++VKK V  K RL 
Sbjct: 243 VAGAMLVAFTVSGLMHEVFFFYLTRARPSWEVTGFFVLHGVCTAVEMVVKKAVSGKVRLR 302

Query: 301 AAVSVPVTLVLIFSTKIWLF 319
             VS  +T+  +  T  WLF
Sbjct: 303 REVSGALTVGFVMVTGGWLF 318

[Close]

BLAST of Bhi11G001154 vs. NCBI nr
Match: XP_038903324.1 (acyl-CoA--sterol O-acyltransferase 1-like [Benincasa hispida])

HSP 1 Score: 632.9 bits (1631), Expect = 1.5e-177
Identity = 320/320 (100.00%), Postives = 320/320 (100.00%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLCYCHTAGKLISPGAGRFLAIAPVVVLFFLLPLQLTSIHLGG 60
           MEGEIINLTKVWLTVCISLCYCHTAGKLISPGAGRFLAIAPVVVLFFLLPLQLTSIHLGG
Sbjct: 1   MEGEIINLTKVWLTVCISLCYCHTAGKLISPGAGRFLAIAPVVVLFFLLPLQLTSIHLGG 60

Query: 61  ATAFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKILQNPSEKSSPKNRSE 120
           ATAFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKILQNPSEKSSPKNRSE
Sbjct: 61  ATAFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKILQNPSEKSSPKNRSE 120

Query: 121 ILNEKPKSLLNYSIKALIVGLMVKLYDYSEFIHPKVLLFIYSWHIYFLLEIILAITQFFG 180
           ILNEKPKSLLNYSIKALIVGLMVKLYDYSEFIHPKVLLFIYSWHIYFLLEIILAITQFFG
Sbjct: 121 ILNEKPKSLLNYSIKALIVGLMVKLYDYSEFIHPKVLLFIYSWHIYFLLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVIGRMWAPL 240
           RNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVIGRMWAPL
Sbjct: 181 RNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVIGRMWAPL 240

Query: 241 PAVMATFLVSAMMHELIFYYLGRMKPNWEPTWFFVIHGIALTVEILVKKMVGEKFRLPAA 300
           PAVMATFLVSAMMHELIFYYLGRMKPNWEPTWFFVIHGIALTVEILVKKMVGEKFRLPAA
Sbjct: 241 PAVMATFLVSAMMHELIFYYLGRMKPNWEPTWFFVIHGIALTVEILVKKMVGEKFRLPAA 300

Query: 301 VSVPVTLVLIFSTKIWLFFL 321
           VSVPVTLVLIFSTKIWLFFL
Sbjct: 301 VSVPVTLVLIFSTKIWLFFL 320

[Close]

BLAST of Bhi11G001154 vs. NCBI nr
Match: XP_023527814.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 564.3 bits (1453), Expect = 6.7e-157
Identity = 278/319 (87.15%), Postives = 296/319 (92.79%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLCYCHTAGKLISPGAGRFLAIAPVVVLFFLLPLQLTSIHLGG 60
           MEGEIIN T VWL VC+SLCYCHTAGK++ PGAGRFLAIAPVV+LFFLLPLQLTS+HLGG
Sbjct: 1   MEGEIINFTMVWLIVCMSLCYCHTAGKILPPGAGRFLAIAPVVLLFFLLPLQLTSVHLGG 60

Query: 61  ATAFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKILQNPSEKSSPKNRSE 120
           ATAFF+AWLANFKLLLFAAG+GPLS TPPLPLRRFLALSCLPIKILQNPSEK SPKN  E
Sbjct: 61  ATAFFIAWLANFKLLLFAAGNGPLSATPPLPLRRFLALSCLPIKILQNPSEKQSPKNHHE 120

Query: 121 ILNEKPKSLLNYSIKALIVGLMVKLYDYSEFIHPKVLLFIYSWHIYFLLEIILAITQFFG 180
            LN++PKS LNY IKALIV LMVKLYDYSEFIHPKV+LFIYSWH+YF LEIILAITQFFG
Sbjct: 121 TLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVIGRMWAPL 240
           R LLGIELEPQF DPY+STSLQDFWGRRWNLMATNILQPT+YKPTVKIA+KV+GR WAPL
Sbjct: 181 RTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTIYKPTVKIAAKVVGRKWAPL 240

Query: 241 PAVMATFLVSAMMHELIFYYLGRMKPNWEPTWFFVIHGIALTVEILVKKMVGEKFRLPAA 300
           P VMATFLVSA+MHELIFYYLGRMKPNWE TWFFVIHG ALTVEILVKK++ EK RLPAA
Sbjct: 241 PGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPAA 300

Query: 301 VSVPVTLVLIFSTKIWLFF 320
           VSVPVTLV IFST IWLFF
Sbjct: 301 VSVPVTLVFIFSTAIWLFF 319

[Close]

BLAST of Bhi11G001154 vs. NCBI nr
Match: XP_022982638.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima])

HSP 1 Score: 563.5 bits (1451), Expect = 1.1e-156
Identity = 278/319 (87.15%), Postives = 296/319 (92.79%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLCYCHTAGKLISPGAGRFLAIAPVVVLFFLLPLQLTSIHLGG 60
           MEGEIIN T VWL VC+SLCYCHTAGK++ PGAGRFLAI+PVV+LFFLLPLQLTS+HLGG
Sbjct: 1   MEGEIINFTMVWLIVCMSLCYCHTAGKILHPGAGRFLAISPVVLLFFLLPLQLTSVHLGG 60

Query: 61  ATAFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKILQNPSEKSSPKNRSE 120
           ATAFF+AWLANFKLLLFAAG+GPLS TPPLPLRRFLALSCLPIKILQNPSEK SPKN  E
Sbjct: 61  ATAFFIAWLANFKLLLFAAGNGPLSATPPLPLRRFLALSCLPIKILQNPSEKQSPKNHHE 120

Query: 121 ILNEKPKSLLNYSIKALIVGLMVKLYDYSEFIHPKVLLFIYSWHIYFLLEIILAITQFFG 180
            LN++PKS LNY IKALIV LMVKLYDYSEFIHPKV+LFIYSWH+YF LEIILAITQFFG
Sbjct: 121 TLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVIGRMWAPL 240
           R LLGIELEPQF DPY+STSLQDFWGRRWNLMATNILQPTVYKPTVKIA+KV+GR WAPL
Sbjct: 181 RTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTVYKPTVKIAAKVVGRKWAPL 240

Query: 241 PAVMATFLVSAMMHELIFYYLGRMKPNWEPTWFFVIHGIALTVEILVKKMVGEKFRLPAA 300
           P VMATFLVSA+MHELIFYYLGRMKPNWE TWFFVIHG ALTVEILVKK++ EK RLPAA
Sbjct: 241 PGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPAA 300

Query: 301 VSVPVTLVLIFSTKIWLFF 320
           VSVPVTLV IFST IWLFF
Sbjct: 301 VSVPVTLVFIFSTAIWLFF 319

[Close]

BLAST of Bhi11G001154 vs. NCBI nr
Match: XP_022934183.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata])

HSP 1 Score: 560.1 bits (1442), Expect = 1.3e-155
Identity = 275/319 (86.21%), Postives = 294/319 (92.16%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLCYCHTAGKLISPGAGRFLAIAPVVVLFFLLPLQLTSIHLGG 60
           MEGE+IN   VWL VC+SLCYCHTAGK++ PGAGRFLAIAPVVVLFFLLPLQLTS+HLGG
Sbjct: 1   MEGEVINFIMVWLIVCMSLCYCHTAGKILPPGAGRFLAIAPVVVLFFLLPLQLTSVHLGG 60

Query: 61  ATAFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKILQNPSEKSSPKNRSE 120
           +TAFF+AWLANFKLLLF AG+GPLS TPPLPLRRFLALSCLPIKILQNPSEK SPKN  E
Sbjct: 61  STAFFIAWLANFKLLLFTAGNGPLSATPPLPLRRFLALSCLPIKILQNPSEKQSPKNHHE 120

Query: 121 ILNEKPKSLLNYSIKALIVGLMVKLYDYSEFIHPKVLLFIYSWHIYFLLEIILAITQFFG 180
            LN++PKS LNY IKALIV LMVKLYDYSEFIHPKV+LFIYSWH+YF LEIILAITQFFG
Sbjct: 121 TLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVIGRMWAPL 240
           R LLGIELEPQF DPY+STSLQDFWGRRWNLMATNILQPT+YKPTVKIA+KV+GR WAPL
Sbjct: 181 RTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTIYKPTVKIAAKVVGRKWAPL 240

Query: 241 PAVMATFLVSAMMHELIFYYLGRMKPNWEPTWFFVIHGIALTVEILVKKMVGEKFRLPAA 300
           P VMATFLVSA+MHELIFYYLGRMKPNWE TWFFVIHG ALTVEILVKK++ EK RLPAA
Sbjct: 241 PGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPAA 300

Query: 301 VSVPVTLVLIFSTKIWLFF 320
           VSVPVTLV IFST IWLFF
Sbjct: 301 VSVPVTLVFIFSTAIWLFF 319

[Close]

BLAST of Bhi11G001154 vs. NCBI nr
Match: XP_022948970.1 (acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata] >KAG6606895.1 Acyl-CoA--sterol O-acyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 543.5 bits (1399), Expect = 1.2e-150
Identity = 268/319 (84.01%), Postives = 291/319 (91.22%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLCYCHTAGKLISPGAGRFLAIAPVVVLFFLLPLQLTSIHLGG 60
           MEGEI+N  KVWLTVCISLCYCH  GKL++PG GRFLA+AP+VVLFFLLPLQLTSIHLGG
Sbjct: 1   MEGEILNFIKVWLTVCISLCYCHATGKLLAPGPGRFLAVAPIVVLFFLLPLQLTSIHLGG 60

Query: 61  ATAFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKILQNPSEKSSPKNRSE 120
           +T+FF+AWLANFKLLLFAAGDGPLS  PPLPLRRFLALSCLPIKILQN  EKS P N   
Sbjct: 61  STSFFIAWLANFKLLLFAAGDGPLSAEPPLPLRRFLALSCLPIKILQN--EKSCPNNNRS 120

Query: 121 ILNEKPKSLLNYSIKALIVGLMVKLYDYSEFIHPKVLLFIYSWHIYFLLEIILAITQFFG 180
               KPKS LNYSIKA+IV LMVKLYDYSEFIHPKV+LFIYSWH+YFLLEIILAITQFFG
Sbjct: 121 DTTLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVIGRMWAPL 240
           RN+LGIELEPQF+DPY+STSLQDFWGRRWNLMATNILQPTVYKP+VKIA+KVIGR WAPL
Sbjct: 181 RNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAPL 240

Query: 241 PAVMATFLVSAMMHELIFYYLGRMKPNWEPTWFFVIHGIALTVEILVKKMVGEKFRLPAA 300
           PAVMATFLVSAMMHELIFYY+GRM+PNWE TWFFVIHG+ALTVEI+VKK++ EK+RLP A
Sbjct: 241 PAVMATFLVSAMMHELIFYYMGRMRPNWECTWFFVIHGVALTVEIMVKKVLPEKWRLPPA 300

Query: 301 VSVPVTLVLIFSTKIWLFF 320
           VSVPVTL  IF T IWLFF
Sbjct: 301 VSVPVTLAFIFPTAIWLFF 317

[Close]

BLAST of Bhi11G001154 vs. ExPASy TrEMBL
Match: A0A6J1J5C4 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111481453 PE=3 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 5.5e-157
Identity = 278/319 (87.15%), Postives = 296/319 (92.79%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLCYCHTAGKLISPGAGRFLAIAPVVVLFFLLPLQLTSIHLGG 60
           MEGEIIN T VWL VC+SLCYCHTAGK++ PGAGRFLAI+PVV+LFFLLPLQLTS+HLGG
Sbjct: 1   MEGEIINFTMVWLIVCMSLCYCHTAGKILHPGAGRFLAISPVVLLFFLLPLQLTSVHLGG 60

Query: 61  ATAFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKILQNPSEKSSPKNRSE 120
           ATAFF+AWLANFKLLLFAAG+GPLS TPPLPLRRFLALSCLPIKILQNPSEK SPKN  E
Sbjct: 61  ATAFFIAWLANFKLLLFAAGNGPLSATPPLPLRRFLALSCLPIKILQNPSEKQSPKNHHE 120

Query: 121 ILNEKPKSLLNYSIKALIVGLMVKLYDYSEFIHPKVLLFIYSWHIYFLLEIILAITQFFG 180
            LN++PKS LNY IKALIV LMVKLYDYSEFIHPKV+LFIYSWH+YF LEIILAITQFFG
Sbjct: 121 TLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVIGRMWAPL 240
           R LLGIELEPQF DPY+STSLQDFWGRRWNLMATNILQPTVYKPTVKIA+KV+GR WAPL
Sbjct: 181 RTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTVYKPTVKIAAKVVGRKWAPL 240

Query: 241 PAVMATFLVSAMMHELIFYYLGRMKPNWEPTWFFVIHGIALTVEILVKKMVGEKFRLPAA 300
           P VMATFLVSA+MHELIFYYLGRMKPNWE TWFFVIHG ALTVEILVKK++ EK RLPAA
Sbjct: 241 PGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPAA 300

Query: 301 VSVPVTLVLIFSTKIWLFF 320
           VSVPVTLV IFST IWLFF
Sbjct: 301 VSVPVTLVFIFSTAIWLFF 319

[Close]

BLAST of Bhi11G001154 vs. ExPASy TrEMBL
Match: A0A6J1F6Y6 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111441427 PE=3 SV=1)

HSP 1 Score: 560.1 bits (1442), Expect = 6.1e-156
Identity = 275/319 (86.21%), Postives = 294/319 (92.16%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLCYCHTAGKLISPGAGRFLAIAPVVVLFFLLPLQLTSIHLGG 60
           MEGE+IN   VWL VC+SLCYCHTAGK++ PGAGRFLAIAPVVVLFFLLPLQLTS+HLGG
Sbjct: 1   MEGEVINFIMVWLIVCMSLCYCHTAGKILPPGAGRFLAIAPVVVLFFLLPLQLTSVHLGG 60

Query: 61  ATAFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKILQNPSEKSSPKNRSE 120
           +TAFF+AWLANFKLLLF AG+GPLS TPPLPLRRFLALSCLPIKILQNPSEK SPKN  E
Sbjct: 61  STAFFIAWLANFKLLLFTAGNGPLSATPPLPLRRFLALSCLPIKILQNPSEKQSPKNHHE 120

Query: 121 ILNEKPKSLLNYSIKALIVGLMVKLYDYSEFIHPKVLLFIYSWHIYFLLEIILAITQFFG 180
            LN++PKS LNY IKALIV LMVKLYDYSEFIHPKV+LFIYSWH+YF LEIILAITQFFG
Sbjct: 121 TLNQEPKSFLNYWIKALIVALMVKLYDYSEFIHPKVILFIYSWHVYFFLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVIGRMWAPL 240
           R LLGIELEPQF DPY+STSLQDFWGRRWNLMATNILQPT+YKPTVKIA+KV+GR WAPL
Sbjct: 181 RTLLGIELEPQFHDPYFSTSLQDFWGRRWNLMATNILQPTIYKPTVKIAAKVVGRKWAPL 240

Query: 241 PAVMATFLVSAMMHELIFYYLGRMKPNWEPTWFFVIHGIALTVEILVKKMVGEKFRLPAA 300
           P VMATFLVSA+MHELIFYYLGRMKPNWE TWFFVIHG ALTVEILVKK++ EK RLPAA
Sbjct: 241 PGVMATFLVSAIMHELIFYYLGRMKPNWELTWFFVIHGFALTVEILVKKVLPEKRRLPAA 300

Query: 301 VSVPVTLVLIFSTKIWLFF 320
           VSVPVTLV IFST IWLFF
Sbjct: 301 VSVPVTLVFIFSTAIWLFF 319

[Close]

BLAST of Bhi11G001154 vs. ExPASy TrEMBL
Match: A0A6J1GBE5 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111452458 PE=3 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 5.9e-151
Identity = 268/319 (84.01%), Postives = 291/319 (91.22%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLCYCHTAGKLISPGAGRFLAIAPVVVLFFLLPLQLTSIHLGG 60
           MEGEI+N  KVWLTVCISLCYCH  GKL++PG GRFLA+AP+VVLFFLLPLQLTSIHLGG
Sbjct: 1   MEGEILNFIKVWLTVCISLCYCHATGKLLAPGPGRFLAVAPIVVLFFLLPLQLTSIHLGG 60

Query: 61  ATAFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKILQNPSEKSSPKNRSE 120
           +T+FF+AWLANFKLLLFAAGDGPLS  PPLPLRRFLALSCLPIKILQN  EKS P N   
Sbjct: 61  STSFFIAWLANFKLLLFAAGDGPLSAEPPLPLRRFLALSCLPIKILQN--EKSCPNNNRS 120

Query: 121 ILNEKPKSLLNYSIKALIVGLMVKLYDYSEFIHPKVLLFIYSWHIYFLLEIILAITQFFG 180
               KPKS LNYSIKA+IV LMVKLYDYSEFIHPKV+LFIYSWH+YFLLEIILAITQFFG
Sbjct: 121 DTTLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVIGRMWAPL 240
           RN+LGIELEPQF+DPY+STSLQDFWGRRWNLMATNILQPTVYKP+VKIA+KVIGR WAPL
Sbjct: 181 RNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAPL 240

Query: 241 PAVMATFLVSAMMHELIFYYLGRMKPNWEPTWFFVIHGIALTVEILVKKMVGEKFRLPAA 300
           PAVMATFLVSAMMHELIFYY+GRM+PNWE TWFFVIHG+ALTVEI+VKK++ EK+RLP A
Sbjct: 241 PAVMATFLVSAMMHELIFYYMGRMRPNWECTWFFVIHGVALTVEIMVKKVLPEKWRLPPA 300

Query: 301 VSVPVTLVLIFSTKIWLFF 320
           VSVPVTL  IF T IWLFF
Sbjct: 301 VSVPVTLAFIFPTAIWLFF 317

[Close]

BLAST of Bhi11G001154 vs. ExPASy TrEMBL
Match: A0A0A0L9S2 (MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G684690 PE=3 SV=1)

HSP 1 Score: 541.6 bits (1394), Expect = 2.2e-150
Identity = 271/320 (84.69%), Postives = 290/320 (90.62%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLCYCHTAGKLISPGAGRFLAIAPVVVLFFLLPLQLTSIHLGG 60
           ME EIINLTKVWLTV ISL YCH   KL++PG  RFLAIAPVV++FFLLPLQLTSIH GG
Sbjct: 28  MEAEIINLTKVWLTVFISLGYCHATAKLLNPGPARFLAIAPVVLIFFLLPLQLTSIHFGG 87

Query: 61  ATAFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKILQNPSEKSSPK-NRS 120
           AT FFV WLA FKLLL  AGDGPLSTTPPLPL RF+A+SCLPIKIL+NPSEKS+PK NR+
Sbjct: 88  ATCFFVTWLATFKLLLLTAGDGPLSTTPPLPLHRFIAISCLPIKILENPSEKSAPKNNRA 147

Query: 121 EILNEKPKSLLNYSIKALIVGLMVKLYDYSEFIHPKVLLFIYSWHIYFLLEIILAITQFF 180
           E LNEKPKS LNYSI+ALIV LMVKLYDYS F HPK++LFIYSWH+Y LLEIILAITQFF
Sbjct: 148 ETLNEKPKSFLNYSIRALIVVLMVKLYDYSHFFHPKLVLFIYSWHVYLLLEIILAITQFF 207

Query: 181 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVIGRMWAP 240
           GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQP VYKPTVKIA++VIGRMWAP
Sbjct: 208 GRNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPAVYKPTVKIAARVIGRMWAP 267

Query: 241 LPAVMATFLVSAMMHELIFYYLGRMKPNWEPTWFFVIHGIALTVEILVKKMVGEKFRLPA 300
           LP VMATFLVSAMMHELIFYYLGRM+PNWE TWFFVIHGIALTVEI++KKM+ EK RLPA
Sbjct: 268 LPGVMATFLVSAMMHELIFYYLGRMRPNWEITWFFVIHGIALTVEIMMKKMLPEKRRLPA 327

Query: 301 AVSVPVTLVLIFSTKIWLFF 320
            VSVPVTLV IFST IWLFF
Sbjct: 328 KVSVPVTLVFIFSTAIWLFF 347

[Close]

BLAST of Bhi11G001154 vs. ExPASy TrEMBL
Match: A0A6J1KAA2 (acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493091 PE=3 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 3.8e-150
Identity = 267/319 (83.70%), Postives = 289/319 (90.60%), Query Frame = 0


Query: 1   MEGEIINLTKVWLTVCISLCYCHTAGKLISPGAGRFLAIAPVVVLFFLLPLQLTSIHLGG 60
           MEGEI+N  KVWLTVCISLCYCH  GKLI PGAGRFLA+AP+VVLFF LPLQLTSIHLGG
Sbjct: 1   MEGEILNFIKVWLTVCISLCYCHATGKLIPPGAGRFLAVAPIVVLFFFLPLQLTSIHLGG 60

Query: 61  ATAFFVAWLANFKLLLFAAGDGPLSTTPPLPLRRFLALSCLPIKILQNPSEKSSPKNRSE 120
           +T+FF+AWLANFKLLLF AGDGPLS  PPLPLRRFLALSCLPIKILQN  EKS P N   
Sbjct: 61  STSFFIAWLANFKLLLFTAGDGPLSAEPPLPLRRFLALSCLPIKILQN--EKSCPNNNRS 120

Query: 121 ILNEKPKSLLNYSIKALIVGLMVKLYDYSEFIHPKVLLFIYSWHIYFLLEIILAITQFFG 180
               KPKS LNYSIKA+IV LMVKLYDYSEFIHPKV+LFIYSWH+YFLLEIILAITQFFG
Sbjct: 121 DTTLKPKSFLNYSIKAVIVALMVKLYDYSEFIHPKVVLFIYSWHLYFLLEIILAITQFFG 180

Query: 181 RNLLGIELEPQFRDPYYSTSLQDFWGRRWNLMATNILQPTVYKPTVKIASKVIGRMWAPL 240
           RN+LGIELEPQF+DPY+STSLQDFWGRRWNLMATNILQPTVYKP+VKIA+KVIGR WAPL
Sbjct: 181 RNVLGIELEPQFQDPYFSTSLQDFWGRRWNLMATNILQPTVYKPSVKIAAKVIGRKWAPL 240

Query: 241 PAVMATFLVSAMMHELIFYYLGRMKPNWEPTWFFVIHGIALTVEILVKKMVGEKFRLPAA 300
           PAVMATFLVSAMMHELIF+Y+GRM+PNWE TWFFVIHG+ALTVEI+VKK++ EK+RLP A
Sbjct: 241 PAVMATFLVSAMMHELIFFYMGRMRPNWECTWFFVIHGVALTVEIMVKKVLPEKWRLPPA 300

Query: 301 VSVPVTLVLIFSTKIWLFF 320
           VSVPVTL  IF T IWLFF
Sbjct: 301 VSVPVTLAFIFPTAIWLFF 317

The following BLAST results are available for this feature:

BLAST of Bhi11G001154 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G51970.1	5.0e-78	50.65	acyl-CoA sterol acyl transferase 1	[more]
AT5G55340.1	2.6e-66	43.44	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55380.1	2.1e-63	42.01	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55350.1	6.0e-63	41.88	MBOAT (membrane bound O-acyl transferase) family protein	[more]
AT5G55360.1	1.6e-60	40.87	MBOAT (membrane bound O-acyl transferase) family protein	[more]

BLAST of Bhi11G001154 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SV07	7.1e-77	50.65	Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE...	[more]
Q9FJ76	3.7e-65	43.44	Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX...	[more]
Q9FJ72	2.9e-62	42.01	Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX...	[more]
Q9FJ75	8.4e-62	41.88	Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX...	[more]
Q9XGY6	3.2e-61	41.25	Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 ...	[more]

BLAST of Bhi11G001154 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038903324.1	1.5e-177	100.00	acyl-CoA--sterol O-acyltransferase 1-like [Benincasa hispida]	[more]
XP_023527814.1	6.7e-157	87.15	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022982638.1	1.1e-156	87.15	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita maxima]	[more]
XP_022934183.1	1.3e-155	86.21	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata]	[more]
XP_022948970.1	1.2e-150	84.01	acyl-CoA--sterol O-acyltransferase 1-like [Cucurbita moschata] >KAG6606895.1 Acy...	[more]

BLAST of Bhi11G001154 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J5C4	5.5e-157	87.15	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1F6Y6	6.1e-156	86.21	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1GBE5	5.9e-151	84.01	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A0A0L9S2	2.2e-150	84.69	MBOAT_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G684690 PE=...	[more]
A0A6J1KAA2	3.8e-150	83.70	acyl-CoA--sterol O-acyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR032805	Wax synthase domain	PFAM	PF13813	MBOAT_2	coord: 189..275 e-value: 4.8E-16 score: 58.8
IPR017088	Wax synthase, Magnoliopsida	PIRSF	PIRSF037006	Wax_synthase	coord: 1..320 e-value: 3.6E-110 score: 366.3
IPR044851	Wax synthase	PANTHER	PTHR31595	LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATED	coord: 2..319
None	No IPR available	PANTHER	PTHR31595:SF46	ACYL-COA--STEROL O-ACYLTRANSFERASE 1	coord: 2..319

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi11M001154	Bhi11M001154	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008374	O-acyltransferase activity