Bhi11G001077 (gene) Wax gourd (B227) v1

Overview


Name	Bhi11G001077
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Pectinesterase
Location	chr11: 37438285 .. 37439109 (-)
RNA-Seq Expression	Bhi11G001077
Synteny	Bhi11G001077

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AATACTCATAATGCAGCAGGAAATGTGAGGAAACGTGAAGATATAACACCAGCAATTGCAGCTCGCATAGAAGGAGACAAAGGGATATTCCATAATTGTGGCTTCATCGGGCTCCAAGATACTTTGTGGGACGGACATGGCCGTCATCTATTCACTCAATGCTACATTGAAGGTGTCATTGATGTCATATCTGGTTTTGGTCAGTCTATCTATAAGGTTAACTTCTAAGTAAATGAAATTCATTTACGAGTCCGGCTTTTCATATCCACAAGTGTCTGAGTCAGTTTACACGCGTGTTTAGACCAATTGTATGAGACGAGAAAGTCTTTTTTGTTTTTTCACATGCTCTCAAATGTCACATTAGGTCAATCTATGATCAATCTTTTTTTAACAAGATATTAAAAGTAGTGGAATCAATAATTAGGTGAACCAGGATATCTCAACAATTAAGTTCACATAACTATAGTACTTTCACCGGTGAGTTGCATTATCAGAAAAATATACAAAGAAGTGTCTTTCATTGCATTAGTTAAATTGAAATATTGATTTTTTGTTTTGTGTTGTTTGATTGCAGAAATGTGTGATAAATATACCAATCAATGTGTATGCTCCAATACTAAATATTGGTTACATAACAGCTCAAGGAAAGAATAATGCAAGTGAAAGCAATGGGTTTGTATTCATTGGATGCACAGTGATGGGAAGTGGGAATGTTTTTCTTGGAAGGGCTTATAGACCTTTCTCCACTGTCATTTTCCATCTATCTTTTCTACCATCTTGCATTAGCCCTGCTGGTTGGAATTCTTGGGAACAACTTGGCCATGA

mRNA sequence

AATACTCATAATGCAGCAGGAAATGTGAGGAAACGTGAAGATATAACACCAGCAATTGCAGCTCGCATAGAAGGAGACAAAGGGATATTCCATAATTGTGGCTTCATCGGGCTCCAAGATACTTTGTGGGACGGACATGGCCGTCATCTATTCACTCAATGCTACATTGAAGGTGTCATTGATGTCATATCTGGTTTTGGTCAGTCTATCTATAAGAAATGTGTGATAAATATACCAATCAATGTGTATGCTCCAATACTAAATATTGGTTACATAACAGCTCAAGGAAAGAATAATGCAAGTGAAAGCAATGGGTTTGTATTCATTGGATGCACAGTGATGGGAAGTGGGAATGTTTTTCTTGGAAGGGCTTATAGACCTTTCTCCACTGTCATTTTCCATCTATCTTTTCTACCATCTTGCATTAGCCCTGCTGGTTGGAATTCTTGGGAACAACTTGGCCATGA

Coding sequence (CDS)

AATACTCATAATGCAGCAGGAAATGTGAGGAAACGTGAAGATATAACACCAGCAATTGCAGCTCGCATAGAAGGAGACAAAGGGATATTCCATAATTGTGGCTTCATCGGGCTCCAAGATACTTTGTGGGACGGACATGGCCGTCATCTATTCACTCAATGCTACATTGAAGGTGTCATTGATGTCATATCTGGTTTTGGTCAGTCTATCTATAAGAAATGTGTGATAAATATACCAATCAATGTGTATGCTCCAATACTAAATATTGGTTACATAACAGCTCAAGGAAAGAATAATGCAAGTGAAAGCAATGGGTTTGTATTCATTGGATGCACAGTGATGGGAAGTGGGAATGTTTTTCTTGGAAGGGCTTATAGACCTTTCTCCACTGTCATTTTCCATCTATCTTTTCTACCATCTTGCATTAGCCCTGCTGGTTGGAATTCTTGGGAACAACTTGGCCATGA

Protein sequence

NTHNAAGNVRKREDITPAIAARIEGDKGIFHNCGFIGLQDTLWDGHGRHLFTQCYIEGVIDVISGFGQSIYKKCVINIPINVYAPILNIGYITAQGKNNASESNGFVFIGCTVMGSGNVFLGRAYRPFSTVIFHLSFLPSCISPAGWNSWEQLGH

Homology

[Close]

BLAST of Bhi11G001077 vs. TAIR 10
Match: AT5G26810.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 162.5 bits (410), Expect = 2.6e-40
Identity = 79/159 (49.69%), Postives = 103/159 (64.78%), Query Frame = 0


Query: 1   NTHNAAGNVRKRE----DITPAIAARIEGDKGIFHNCGFIGLQDTLWDGHGRHLFTQCYI 60
           NT+N   ++ K      DI PA+A  + GDK  F+NC F+GLQDT+WD  GRH F  CYI
Sbjct: 80  NTYNRLTSLTKANGMSWDIKPAVAISVYGDKSAFYNCDFLGLQDTVWDNLGRHHFKNCYI 139

Query: 61  EGVIDVISGFGQSIYKKCVINIPINVYAPILNIGYITAQGKNNASESNGFVFIGCTVMGS 120
           EG ID I G GQS+Y+ C IN      A  ++ GYITAQG+++ S+ +GFVF+  +V GS
Sbjct: 140 EGAIDFIFGSGQSVYEDCHINATAGALASKVSFGYITAQGRSSDSDPSGFVFLRGSVSGS 199

Query: 121 GNVFLGRAYRPFSTVIFHLSFLPSCISPAGWNSWEQLGH 156
            +V+LGRAY PFS VIF  + L S + P GW SW   G+
Sbjct: 200 TSVYLGRAYGPFSRVIFIQTDLSSVVHPEGWYSWHYGGY 238

[Close]

BLAST of Bhi11G001077 vs. TAIR 10
Match: AT5G18990.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 157.5 bits (397), Expect = 8.5e-39
Identity = 76/156 (48.72%), Postives = 100/156 (64.10%), Query Frame = 0


Query: 1   NTHNAAGNVR-KREDITPAIAARIEGDKGIFHNCGFIGLQDTLWDGHGRHLFTQCYIEGV 60
           N++N   N +  +    PA+AA I GDK  F++ GF G+QDTLWD  GRH F +C I+G 
Sbjct: 121 NSYNFPSNGKINKNPRVPAVAAFIGGDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGA 180

Query: 61  IDVISGFGQSIYKKCVINIPINVYAPILNIGYITAQGKNNASESNGFVFIGCTVMGSGNV 120
           +D I G GQSIY+ CVI +      P +  GYITAQG+ NA+++NGFVFI C V G G  
Sbjct: 181 VDFILGSGQSIYQSCVIQVLGGQLGPGVT-GYITAQGRTNANDANGFVFINCLVHGFGKA 240

Query: 121 FLGRAYRPFSTVIFHLSFLPSCISPAGWNSWEQLGH 156
           +LGRA+RP+S VIF+ S L   + P GW  W   G+
Sbjct: 241 YLGRAWRPYSRVIFYNSNLTDVVDPLGWWEWNYQGY 275

[Close]

BLAST of Bhi11G001077 vs. TAIR 10
Match: AT3G24130.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 156.0 bits (393), Expect = 2.5e-38
Identity = 73/140 (52.14%), Postives = 94/140 (67.14%), Query Frame = 0


Query: 16  TPAIAARIEGDKGIFHNCGFIGLQDTLWDGHGRHLFTQCYIEGVIDVISGFGQSIYKKCV 75
           TPA+AA I GDK  F++ GF G+QDTLWD  GRH F +C I+G +D I G GQSIY+ CV
Sbjct: 142 TPAVAALIGGDKSAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQSCV 201

Query: 76  INIPINVYAPILNIGYITAQGKNNASESNGFVFIGCTVMGSGNVFLGRAYRPFSTVIFHL 135
           I +      P L  GYITAQG+ N  ++NGF+FI C V G+G  FLGR +R +S VIF+ 
Sbjct: 202 IQVLGGQLEPGL-AGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSRVIFYN 261

Query: 136 SFLPSCISPAGWNSWEQLGH 156
           S L   + P GW++W  +GH
Sbjct: 262 SNLTDVVVPEGWDAWNFVGH 280

[Close]

BLAST of Bhi11G001077 vs. TAIR 10
Match: AT2G47280.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 144.1 bits (362), Expect = 9.7e-35
Identity = 68/155 (43.87%), Postives = 97/155 (62.58%), Query Frame = 0


Query: 1   NTHNAAGNVRKREDITPAIAARIEGDKGIFHNCGFIGLQDTLWDGHGRHLFTQCYIEGVI 60
           NT+N   N   + +I PA+AAR+ GD+ +  +  F+GLQDTL+DG GRH + +C I G I
Sbjct: 127 NTYNIVPN--NKREIVPAVAARMLGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGI 186

Query: 61  DVISGFGQSIYKKCVINIPINVYAPILNIGYITAQGKNNASESNGFVFIGCTVMGSGNVF 120
           D I G+GQS++K+C +N+ + +YAP    G ITA  + + S+  GFVF  CTV G G   
Sbjct: 187 DFIFGYGQSLFKECTLNMTLGIYAPDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTL 246

Query: 121 LGRAYRPFSTVIFHLSFLPSCISPAGWNSWEQLGH 156
           LGRA+   + VIF  S L   + P GW++W   G+
Sbjct: 247 LGRAWGSNARVIFDRSRLSDVVLPIGWDAWRAKGN 279

[Close]

BLAST of Bhi11G001077 vs. TAIR 10
Match: AT2G36710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 135.6 bits (340), Expect = 3.5e-32
Identity = 61/133 (45.86%), Postives = 86/133 (64.66%), Query Frame = 0


Query: 18  AIAARIEGDKGIFHNCGFIGLQDTLWDGHGRHLFTQCYIEGVIDVISGFGQSIYKKCVIN 77
           A+A RIEGD+  F+ CGF G QDTL D  GRH F +C+I+G ID I G G+S+Y+ C IN
Sbjct: 201 AVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTIN 260

Query: 78  IPINVYAPILNIGYITAQGKNNASESNGFVFIGCTVMGSGNVFLGRAYRPFSTVIFHLSF 137
                    +  G ITAQG+ +  E +GF F+ C + GSG + LGRA+  ++TV+F  ++
Sbjct: 261 SIAKGNTSGVT-GSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYATVVFSNTY 320

Query: 138 LPSCISPAGWNSW 151
           +   I+P GWN+W
Sbjct: 321 MSGIITPEGWNNW 332

[Close]

BLAST of Bhi11G001077 vs. ExPASy Swiss-Prot
Match: O04953 (Putative pectinesterase 52 OS=Arabidopsis thaliana OX=3702 GN=PME52 PE=2 SV=2)

HSP 1 Score: 162.5 bits (410), Expect = 3.7e-39
Identity = 79/159 (49.69%), Postives = 103/159 (64.78%), Query Frame = 0


Query: 1   NTHNAAGNVRKRE----DITPAIAARIEGDKGIFHNCGFIGLQDTLWDGHGRHLFTQCYI 60
           NT+N   ++ K      DI PA+A  + GDK  F+NC F+GLQDT+WD  GRH F  CYI
Sbjct: 80  NTYNRLTSLTKANGMSWDIKPAVAISVYGDKSAFYNCDFLGLQDTVWDNLGRHHFKNCYI 139

Query: 61  EGVIDVISGFGQSIYKKCVINIPINVYAPILNIGYITAQGKNNASESNGFVFIGCTVMGS 120
           EG ID I G GQS+Y+ C IN      A  ++ GYITAQG+++ S+ +GFVF+  +V GS
Sbjct: 140 EGAIDFIFGSGQSVYEDCHINATAGALASKVSFGYITAQGRSSDSDPSGFVFLRGSVSGS 199

Query: 121 GNVFLGRAYRPFSTVIFHLSFLPSCISPAGWNSWEQLGH 156
            +V+LGRAY PFS VIF  + L S + P GW SW   G+
Sbjct: 200 TSVYLGRAYGPFSRVIFIQTDLSSVVHPEGWYSWHYGGY 238

[Close]

BLAST of Bhi11G001077 vs. ExPASy Swiss-Prot
Match: Q3E9D3 (Probable pectinesterase 55 OS=Arabidopsis thaliana OX=3702 GN=PME55 PE=2 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 1.2e-37
Identity = 76/156 (48.72%), Postives = 100/156 (64.10%), Query Frame = 0


Query: 1   NTHNAAGNVR-KREDITPAIAARIEGDKGIFHNCGFIGLQDTLWDGHGRHLFTQCYIEGV 60
           N++N   N +  +    PA+AA I GDK  F++ GF G+QDTLWD  GRH F +C I+G 
Sbjct: 121 NSYNFPSNGKINKNPRVPAVAAFIGGDKSAFYSVGFAGIQDTLWDSDGRHYFHRCTIQGA 180

Query: 61  IDVISGFGQSIYKKCVINIPINVYAPILNIGYITAQGKNNASESNGFVFIGCTVMGSGNV 120
           +D I G GQSIY+ CVI +      P +  GYITAQG+ NA+++NGFVFI C V G G  
Sbjct: 181 VDFILGSGQSIYQSCVIQVLGGQLGPGVT-GYITAQGRTNANDANGFVFINCLVHGFGKA 240

Query: 121 FLGRAYRPFSTVIFHLSFLPSCISPAGWNSWEQLGH 156
           +LGRA+RP+S VIF+ S L   + P GW  W   G+
Sbjct: 241 YLGRAWRPYSRVIFYNSNLTDVVDPLGWWEWNYQGY 275

[Close]

BLAST of Bhi11G001077 vs. ExPASy Swiss-Prot
Match: Q4PSN0 (Probable pectinesterase 29 OS=Arabidopsis thaliana OX=3702 GN=PME29 PE=2 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 3.5e-37
Identity = 73/140 (52.14%), Postives = 94/140 (67.14%), Query Frame = 0


Query: 16  TPAIAARIEGDKGIFHNCGFIGLQDTLWDGHGRHLFTQCYIEGVIDVISGFGQSIYKKCV 75
           TPA+AA I GDK  F++ GF G+QDTLWD  GRH F +C I+G +D I G GQSIY+ CV
Sbjct: 142 TPAVAALIGGDKSAFYSVGFAGIQDTLWDFDGRHYFHRCTIQGAVDFIFGTGQSIYQSCV 201

Query: 76  INIPINVYAPILNIGYITAQGKNNASESNGFVFIGCTVMGSGNVFLGRAYRPFSTVIFHL 135
           I +      P L  GYITAQG+ N  ++NGF+FI C V G+G  FLGR +R +S VIF+ 
Sbjct: 202 IQVLGGQLEPGL-AGYITAQGRTNPYDANGFIFINCLVYGTGMAFLGRPWRGYSRVIFYN 261

Query: 136 SFLPSCISPAGWNSWEQLGH 156
           S L   + P GW++W  +GH
Sbjct: 262 SNLTDVVVPEGWDAWNFVGH 280

[Close]

BLAST of Bhi11G001077 vs. ExPASy Swiss-Prot
Match: Q4PSQ5 (Probable pectinesterase 66 OS=Arabidopsis thaliana OX=3702 GN=PME66 PE=2 SV=2)

HSP 1 Score: 144.1 bits (362), Expect = 1.4e-33
Identity = 68/155 (43.87%), Postives = 97/155 (62.58%), Query Frame = 0


Query: 1   NTHNAAGNVRKREDITPAIAARIEGDKGIFHNCGFIGLQDTLWDGHGRHLFTQCYIEGVI 60
           NT+N   N   + +I PA+AAR+ GD+ +  +  F+GLQDTL+DG GRH + +C I G I
Sbjct: 127 NTYNIVPN--NKREIVPAVAARMLGDRYVVTDSSFVGLQDTLFDGKGRHYYKRCIISGGI 186

Query: 61  DVISGFGQSIYKKCVINIPINVYAPILNIGYITAQGKNNASESNGFVFIGCTVMGSGNVF 120
           D I G+GQS++K+C +N+ + +YAP    G ITA  + + S+  GFVF  CTV G G   
Sbjct: 187 DFIFGYGQSLFKECTLNMTLGIYAPDNPYGTITAHQRPSPSDEGGFVFSDCTVTGVGKTL 246

Query: 121 LGRAYRPFSTVIFHLSFLPSCISPAGWNSWEQLGH 156
           LGRA+   + VIF  S L   + P GW++W   G+
Sbjct: 247 LGRAWGSNARVIFDRSRLSDVVLPIGWDAWRAKGN 279

[Close]

BLAST of Bhi11G001077 vs. ExPASy Swiss-Prot
Match: Q9ZQA3 (Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 4.9e-31
Identity = 61/133 (45.86%), Postives = 86/133 (64.66%), Query Frame = 0


Query: 18  AIAARIEGDKGIFHNCGFIGLQDTLWDGHGRHLFTQCYIEGVIDVISGFGQSIYKKCVIN 77
           A+A RIEGD+  F+ CGF G QDTL D  GRH F +C+I+G ID I G G+S+Y+ C IN
Sbjct: 201 AVALRIEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTIN 260

Query: 78  IPINVYAPILNIGYITAQGKNNASESNGFVFIGCTVMGSGNVFLGRAYRPFSTVIFHLSF 137
                    +  G ITAQG+ +  E +GF F+ C + GSG + LGRA+  ++TV+F  ++
Sbjct: 261 SIAKGNTSGVT-GSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYATVVFSNTY 320

Query: 138 LPSCISPAGWNSW 151
           +   I+P GWN+W
Sbjct: 321 MSGIITPEGWNNW 332

[Close]

BLAST of Bhi11G001077 vs. NCBI nr
Match: XP_038904330.1 (putative pectinesterase 52 [Benincasa hispida])

HSP 1 Score: 332.8 bits (852), Expect = 1.6e-87
Identity = 155/155 (100.00%), Postives = 155/155 (100.00%), Query Frame = 0


Query: 1   NTHNAAGNVRKREDITPAIAARIEGDKGIFHNCGFIGLQDTLWDGHGRHLFTQCYIEGVI 60
           NTHNAAGNVRKREDITPAIAARIEGDKGIFHNCGFIGLQDTLWDGHGRHLFTQCYIEGVI
Sbjct: 197 NTHNAAGNVRKREDITPAIAARIEGDKGIFHNCGFIGLQDTLWDGHGRHLFTQCYIEGVI 256

Query: 61  DVISGFGQSIYKKCVINIPINVYAPILNIGYITAQGKNNASESNGFVFIGCTVMGSGNVF 120
           DVISGFGQSIYKKCVINIPINVYAPILNIGYITAQGKNNASESNGFVFIGCTVMGSGNVF
Sbjct: 257 DVISGFGQSIYKKCVINIPINVYAPILNIGYITAQGKNNASESNGFVFIGCTVMGSGNVF 316

Query: 121 LGRAYRPFSTVIFHLSFLPSCISPAGWNSWEQLGH 156
           LGRAYRPFSTVIFHLSFLPSCISPAGWNSWEQLGH
Sbjct: 317 LGRAYRPFSTVIFHLSFLPSCISPAGWNSWEQLGH 351

[Close]

BLAST of Bhi11G001077 vs. NCBI nr
Match: KAA0038126.1 (putative pectinesterase 55 [Cucumis melo var. makuwa] >TYK20481.1 putative pectinesterase 55 [Cucumis melo var. makuwa])

HSP 1 Score: 278.5 bits (711), Expect = 3.5e-71
Identity = 128/155 (82.58%), Postives = 138/155 (89.03%), Query Frame = 0


Query: 1   NTHNAAGNVRKREDITPAIAARIEGDKGIFHNCGFIGLQDTLWDGHGRHLFTQCYIEGVI 60
           NT+NA G+V +REDI PAIAA I+GDK IFH CGFIGLQDTLWDG GRHLFTQCYIEGVI
Sbjct: 44  NTYNALGSVTRREDIKPAIAALIQGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVI 103

Query: 61  DVISGFGQSIYKKCVINIPINVYAPILNIGYITAQGKNNASESNGFVFIGCTVMGSGNVF 120
           DVISGFGQSIYK+CVINIPIN YAP+LN G+ITAQGK N SES+GFVFI C V GSG VF
Sbjct: 104 DVISGFGQSIYKECVINIPINAYAPLLNEGFITAQGKENPSESSGFVFIRCIVKGSGKVF 163

Query: 121 LGRAYRPFSTVIFHLSFLPSCISPAGWNSWEQLGH 156
           LGRAYRPFSTVIFHL FLPSCI PAGWNSW+Q+GH
Sbjct: 164 LGRAYRPFSTVIFHLCFLPSCIDPAGWNSWQQVGH 198

[Close]

BLAST of Bhi11G001077 vs. NCBI nr
Match: XP_011652807.1 (probable pectinesterase 55 [Cucumis sativus])

HSP 1 Score: 274.6 bits (701), Expect = 5.1e-70
Identity = 125/155 (80.65%), Postives = 138/155 (89.03%), Query Frame = 0


Query: 1   NTHNAAGNVRKREDITPAIAARIEGDKGIFHNCGFIGLQDTLWDGHGRHLFTQCYIEGVI 60
           NT+NA G+V +REDI PA+AA I+GDK IFH CGFIGLQDTLWDG GRHLFTQCYIEGVI
Sbjct: 86  NTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVI 145

Query: 61  DVISGFGQSIYKKCVINIPINVYAPILNIGYITAQGKNNASESNGFVFIGCTVMGSGNVF 120
           DVISGFGQSIYK+CVINIP+N YAP+LN G+ITAQGK N +ES+GFVF+ C V GSGNVF
Sbjct: 146 DVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVF 205

Query: 121 LGRAYRPFSTVIFHLSFLPSCISPAGWNSWEQLGH 156
           LGRAYRPFSTVIFHL FLPSCI+PAGWNSW Q GH
Sbjct: 206 LGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGH 240

[Close]

BLAST of Bhi11G001077 vs. NCBI nr
Match: KAE8650757.1 (hypothetical protein Csa_017626 [Cucumis sativus])

HSP 1 Score: 274.6 bits (701), Expect = 5.1e-70
Identity = 125/155 (80.65%), Postives = 138/155 (89.03%), Query Frame = 0


Query: 1   NTHNAAGNVRKREDITPAIAARIEGDKGIFHNCGFIGLQDTLWDGHGRHLFTQCYIEGVI 60
           NT+NA G+V +REDI PA+AA I+GDK IFH CGFIGLQDTLWDG GRHLFTQCYIEGVI
Sbjct: 138 NTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVI 197

Query: 61  DVISGFGQSIYKKCVINIPINVYAPILNIGYITAQGKNNASESNGFVFIGCTVMGSGNVF 120
           DVISGFGQSIYK+CVINIP+N YAP+LN G+ITAQGK N +ES+GFVF+ C V GSGNVF
Sbjct: 198 DVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVF 257

Query: 121 LGRAYRPFSTVIFHLSFLPSCISPAGWNSWEQLGH 156
           LGRAYRPFSTVIFHL FLPSCI+PAGWNSW Q GH
Sbjct: 258 LGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGH 292

[Close]

BLAST of Bhi11G001077 vs. NCBI nr
Match: XP_016900403.1 (PREDICTED: probable pectinesterase 55 [Cucumis melo])

HSP 1 Score: 259.6 bits (662), Expect = 1.7e-65
Identity = 124/171 (72.51%), Postives = 135/171 (78.95%), Query Frame = 0


Query: 1   NTHNAAGNVRKREDITPAIAARIEGDKGIFHNCGFIGLQDTLWDGHGRHLFTQCYIEGVI 60
           NT+NA G+V +REDI PAIAA I+GDK IFH CGFIGLQDTLWDG GRHLFTQCYIEGVI
Sbjct: 124 NTYNALGSVTRREDIKPAIAALIQGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVI 183

Query: 61  DVISGFGQSI----------------YKKCVINIPINVYAPILNIGYITAQGKNNASESN 120
           DVISGFG  I                 ++CVINIPIN YAP+LN G+ITAQGK N SES+
Sbjct: 184 DVISGFGHQIEVLIFFLFVCLFVLCLTQECVINIPINAYAPLLNEGFITAQGKENPSESS 243

Query: 121 GFVFIGCTVMGSGNVFLGRAYRPFSTVIFHLSFLPSCISPAGWNSWEQLGH 156
           GFVFI C V GSG VFLGRAYRPFSTVIFHL FLPSCI PAGWNSW+Q+GH
Sbjct: 244 GFVFIRCIVKGSGKVFLGRAYRPFSTVIFHLCFLPSCIDPAGWNSWQQVGH 294

[Close]

BLAST of Bhi11G001077 vs. ExPASy TrEMBL
Match: A0A5D3DA76 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00550 PE=4 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 1.7e-71
Identity = 128/155 (82.58%), Postives = 138/155 (89.03%), Query Frame = 0


Query: 1   NTHNAAGNVRKREDITPAIAARIEGDKGIFHNCGFIGLQDTLWDGHGRHLFTQCYIEGVI 60
           NT+NA G+V +REDI PAIAA I+GDK IFH CGFIGLQDTLWDG GRHLFTQCYIEGVI
Sbjct: 44  NTYNALGSVTRREDIKPAIAALIQGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVI 103

Query: 61  DVISGFGQSIYKKCVINIPINVYAPILNIGYITAQGKNNASESNGFVFIGCTVMGSGNVF 120
           DVISGFGQSIYK+CVINIPIN YAP+LN G+ITAQGK N SES+GFVFI C V GSG VF
Sbjct: 104 DVISGFGQSIYKECVINIPINAYAPLLNEGFITAQGKENPSESSGFVFIRCIVKGSGKVF 163

Query: 121 LGRAYRPFSTVIFHLSFLPSCISPAGWNSWEQLGH 156
           LGRAYRPFSTVIFHL FLPSCI PAGWNSW+Q+GH
Sbjct: 164 LGRAYRPFSTVIFHLCFLPSCIDPAGWNSWQQVGH 198

[Close]

BLAST of Bhi11G001077 vs. ExPASy TrEMBL
Match: A0A0A0LB12 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G536660 PE=4 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 2.5e-70
Identity = 125/155 (80.65%), Postives = 138/155 (89.03%), Query Frame = 0


Query: 1   NTHNAAGNVRKREDITPAIAARIEGDKGIFHNCGFIGLQDTLWDGHGRHLFTQCYIEGVI 60
           NT+NA G+V +REDI PA+AA I+GDK IFH CGFIGLQDTLWDG GRHLFTQCYIEGVI
Sbjct: 138 NTYNARGSVMRREDIKPALAALIQGDKVIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVI 197

Query: 61  DVISGFGQSIYKKCVINIPINVYAPILNIGYITAQGKNNASESNGFVFIGCTVMGSGNVF 120
           DVISGFGQSIYK+CVINIP+N YAP+LN G+ITAQGK N +ES+GFVF+ C V GSGNVF
Sbjct: 198 DVISGFGQSIYKECVINIPVNAYAPLLNEGFITAQGKENPNESSGFVFLRCIVQGSGNVF 257

Query: 121 LGRAYRPFSTVIFHLSFLPSCISPAGWNSWEQLGH 156
           LGRAYRPFSTVIFHL FLPSCI+PAGWNSW Q GH
Sbjct: 258 LGRAYRPFSTVIFHLCFLPSCINPAGWNSWLQAGH 292

[Close]

BLAST of Bhi11G001077 vs. ExPASy TrEMBL
Match: A0A1S4DWQ1 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103490078 PE=4 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 8.3e-66
Identity = 124/171 (72.51%), Postives = 135/171 (78.95%), Query Frame = 0


Query: 1   NTHNAAGNVRKREDITPAIAARIEGDKGIFHNCGFIGLQDTLWDGHGRHLFTQCYIEGVI 60
           NT+NA G+V +REDI PAIAA I+GDK IFH CGFIGLQDTLWDG GRHLFTQCYIEGVI
Sbjct: 124 NTYNALGSVTRREDIKPAIAALIQGDKAIFHKCGFIGLQDTLWDGPGRHLFTQCYIEGVI 183

Query: 61  DVISGFGQSI----------------YKKCVINIPINVYAPILNIGYITAQGKNNASESN 120
           DVISGFG  I                 ++CVINIPIN YAP+LN G+ITAQGK N SES+
Sbjct: 184 DVISGFGHQIEVLIFFLFVCLFVLCLTQECVINIPINAYAPLLNEGFITAQGKENPSESS 243

Query: 121 GFVFIGCTVMGSGNVFLGRAYRPFSTVIFHLSFLPSCISPAGWNSWEQLGH 156
           GFVFI C V GSG VFLGRAYRPFSTVIFHL FLPSCI PAGWNSW+Q+GH
Sbjct: 244 GFVFIRCIVKGSGKVFLGRAYRPFSTVIFHLCFLPSCIDPAGWNSWQQVGH 294

[Close]

BLAST of Bhi11G001077 vs. ExPASy TrEMBL
Match: A0A6J1GAV4 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111452500 PE=4 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 1.1e-62
Identity = 106/150 (70.67%), Postives = 135/150 (90.00%), Query Frame = 0


Query: 6   AGNVRKREDITPAIAARIEGDKGIFHNCGFIGLQDTLWDGHGRHLFTQCYIEGVIDVISG 65
           +G+V+++EDI PA+AA IEGDKGIFH CGF+GLQDTLWDG+GRH +T+CYIEGV DVISG
Sbjct: 43  SGSVKRQEDIAPAVAALIEGDKGIFHKCGFVGLQDTLWDGNGRHRYTECYIEGVTDVISG 102

Query: 66  FGQSIYKKCVINIPINVYAPILNIGYITAQGKNNASESNGFVFIGCTVMGSGNVFLGRAY 125
            GQSIY+ C INIP+++YAP++  GYITAQGK ++S++NGFVFIGC+V+GSG V+LGRAY
Sbjct: 103 AGQSIYENCEINIPVDLYAPVIGHGYITAQGKEDSSQTNGFVFIGCSVIGSGTVYLGRAY 162

Query: 126 RPFSTVIFHLSFLPSCISPAGWNSWEQLGH 156
           RPFSTVI++ SFL +CI+PAGW+ WEQ+GH
Sbjct: 163 RPFSTVIYYSSFLSACINPAGWDPWEQVGH 192

[Close]

BLAST of Bhi11G001077 vs. ExPASy TrEMBL
Match: A0A6J1DWQ8 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111023848 PE=4 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 1.5e-59
Identity = 109/155 (70.32%), Postives = 127/155 (81.94%), Query Frame = 0


Query: 1   NTHNAAGNVRKREDITPAIAARIEGDKGIFHNCGFIGLQDTLWDGHGRHLFTQCYIEGVI 60
           NT+NA G+VR++EDI PA+AA I GDK  F+ C FIGLQDTLWDG GRH F  CYIEGVI
Sbjct: 135 NTYNAPGSVRRQEDIAPAVAALIMGDKASFYECRFIGLQDTLWDGPGRHHFVNCYIEGVI 194

Query: 61  DVISGFGQSIYKKCVINIPINVYAPILNIGYITAQGKNNASESNGFVFIGCTVMGSGNVF 120
           DVISG GQSIY+KC+IN+PI+ YAPIL+ GYITAQGK N +E +GFVF  C+V+G+G V+
Sbjct: 195 DVISGSGQSIYEKCMINVPIHQYAPILSYGYITAQGKENPNEGSGFVFNACSVVGNGRVY 254

Query: 121 LGRAYRPFSTVIFHLSFLPSCISPAGWNSWEQLGH 156
           LGRAYRPFSTVIFH SFL   I PAGWN W Q+GH
Sbjct: 255 LGRAYRPFSTVIFHNSFLAGFIDPAGWNPWGQVGH 289

The following BLAST results are available for this feature:

BLAST of Bhi11G001077 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G26810.1	2.6e-40	49.69	Pectin lyase-like superfamily protein	[more]
AT5G18990.1	8.5e-39	48.72	Pectin lyase-like superfamily protein	[more]
AT3G24130.1	2.5e-38	52.14	Pectin lyase-like superfamily protein	[more]
AT2G47280.1	9.7e-35	43.87	Pectin lyase-like superfamily protein	[more]
AT2G36710.1	3.5e-32	45.86	Pectin lyase-like superfamily protein	[more]

BLAST of Bhi11G001077 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04953	3.7e-39	49.69	Putative pectinesterase 52 OS=Arabidopsis thaliana OX=3702 GN=PME52 PE=2 SV=2	[more]
Q3E9D3	1.2e-37	48.72	Probable pectinesterase 55 OS=Arabidopsis thaliana OX=3702 GN=PME55 PE=2 SV=1	[more]
Q4PSN0	3.5e-37	52.14	Probable pectinesterase 29 OS=Arabidopsis thaliana OX=3702 GN=PME29 PE=2 SV=1	[more]
Q4PSQ5	1.4e-33	43.87	Probable pectinesterase 66 OS=Arabidopsis thaliana OX=3702 GN=PME66 PE=2 SV=2	[more]
Q9ZQA3	4.9e-31	45.86	Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1	[more]

BLAST of Bhi11G001077 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038904330.1	1.6e-87	100.00	putative pectinesterase 52 [Benincasa hispida]	[more]
KAA0038126.1	3.5e-71	82.58	putative pectinesterase 55 [Cucumis melo var. makuwa] >TYK20481.1 putative pecti...	[more]
XP_011652807.1	5.1e-70	80.65	probable pectinesterase 55 [Cucumis sativus]	[more]
KAE8650757.1	5.1e-70	80.65	hypothetical protein Csa_017626 [Cucumis sativus]	[more]
XP_016900403.1	1.7e-65	72.51	PREDICTED: probable pectinesterase 55 [Cucumis melo]	[more]

BLAST of Bhi11G001077 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DA76	1.7e-71	82.58	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00550...	[more]
A0A0A0LB12	2.5e-70	80.65	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G536660 PE=4 SV=1	[more]
A0A1S4DWQ1	8.3e-66	72.51	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103490078 PE=4 SV=1	[more]
A0A6J1GAV4	1.1e-62	70.67	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111452500 PE=4 SV=1	[more]
A0A6J1DWQ8	1.5e-59	70.32	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111023848 PE=4 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 16..151 e-value: 1.6E-26 score: 92.7
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 1..154 e-value: 2.9E-43 score: 150.4
None	No IPR available	PANTHER	PTHR31321:SF82	PECTINESTERASE	coord: 7..155
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 7..155
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 3..151

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi11M001077	Bhi11M001077	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0045490	pectin catabolic process
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity