Bhi11G000969 (gene) Wax gourd (B227) v1

Overview


Name	Bhi11G000969
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Lipoyl(octanoyl) transferase
Location	chr11: 33918723 .. 33919029 (-)
RNA-Seq Expression	Bhi11G000969
Synteny	Bhi11G000969

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCTTCATTGGTACCTCAGGGCACTTGAGGAGGTCATCATACGCGTTCTTTCCTCGACATTTTCGATCAATGCTCATCGAATCGATGGTTTAACAGGTGTCTGGGCAGGTATTCCTTGTAGAAATTTGTATATACCATATTCCAAATGCTTTTTCTCTTCTATTTTTGTATGTAGCATATTCTAAATGCTATATTCATTCCTCGCTACTTCGACATTCGACATAACGAATTGAATTTCTTAAATGATTTTCCTTTGCAGGAGACCAGAAACTGGAAGCCATTGGAATTAGGGTATCTAAATAG

mRNA sequence

ATGGATCTTCATTGGTACCTCAGGGCACTTGAGGAGGTCATCATACGCGTTCTTTCCTCGACATTTTCGATCAATGCTCATCGAATCGATGGTTTAACAGGTGTCTGGGCAGGAGACCAGAAACTGGAAGCCATTGGAATTAGGGTATCTAAATAG

Coding sequence (CDS)

ATGGATCTTCATTGGTACCTCAGGGCACTTGAGGAGGTCATCATACGCGTTCTTTCCTCGACATTTTCGATCAATGCTCATCGAATCGATGGTTTAACAGGTGTCTGGGCAGGAGACCAGAAACTGGAAGCCATTGGAATTAGGGTATCTAAATAG

Protein sequence

MDLHWYLRALEEVIIRVLSSTFSINAHRIDGLTGVWAGDQKLEAIGIRVSK

Homology

[Close]

BLAST of Bhi11G000969 vs. TAIR 10
Match: AT4G31050.1 (Biotin/lipoate A/B protein ligase family )

HSP 1 Score: 90.1 bits (222), Expect = 5.5e-19
Identity = 41/51 (80.39%), Postives = 46/51 (90.20%), Query Frame = 0


Query: 1   MDLHWYLRALEEVIIRVLSSTFSINAHRIDGLTGVWAGDQKLEAIGIRVSK 52
           MDLHWYLR LEE++IRVLSSTFSI A R+DGLTGVW G+QK+ AIGIRVSK
Sbjct: 146 MDLHWYLRMLEEIVIRVLSSTFSIKASRLDGLTGVWVGNQKVAAIGIRVSK 196

[Close]

BLAST of Bhi11G000969 vs. TAIR 10
Match: AT1G47578.1 (Biotin/lipoate A/B protein ligase family )

HSP 1 Score: 87.0 bits (214), Expect = 4.6e-18
Identity = 39/51 (76.47%), Postives = 45/51 (88.24%), Query Frame = 0


Query: 1   MDLHWYLRALEEVIIRVLSSTFSINAHRIDGLTGVWAGDQKLEAIGIRVSK 52
           MDLHWYLR LEEV+IRVLSS F+INA R+DG TGVW G++K+ AIGIRVSK
Sbjct: 148 MDLHWYLRKLEEVVIRVLSSAFAINASRLDGFTGVWVGNKKMAAIGIRVSK 198

[Close]

BLAST of Bhi11G000969 vs. ExPASy Swiss-Prot
Match: Q948J9 (Octanoyltransferase LIP2p, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LIP2P PE=1 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 7.7e-18
Identity = 41/51 (80.39%), Postives = 46/51 (90.20%), Query Frame = 0


Query: 1   MDLHWYLRALEEVIIRVLSSTFSINAHRIDGLTGVWAGDQKLEAIGIRVSK 52
           MDLHWYLR LEE++IRVLSSTFSI A R+DGLTGVW G+QK+ AIGIRVSK
Sbjct: 146 MDLHWYLRMLEEIVIRVLSSTFSIKASRLDGLTGVWVGNQKVAAIGIRVSK 196

[Close]

BLAST of Bhi11G000969 vs. ExPASy Swiss-Prot
Match: P0C7R2 (Octanoyltransferase LIP2p2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LIP2P2 PE=1 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 6.5e-17
Identity = 39/51 (76.47%), Postives = 45/51 (88.24%), Query Frame = 0


Query: 1   MDLHWYLRALEEVIIRVLSSTFSINAHRIDGLTGVWAGDQKLEAIGIRVSK 52
           MDLHWYLR LEEV+IRVLSS F+INA R+DG TGVW G++K+ AIGIRVSK
Sbjct: 148 MDLHWYLRKLEEVVIRVLSSAFAINASRLDGFTGVWVGNKKMAAIGIRVSK 198

[Close]

BLAST of Bhi11G000969 vs. ExPASy Swiss-Prot
Match: Q7ND22 (Octanoyltransferase OS=Gloeobacter violaceus (strain ATCC 29082 / PCC 7421) OX=251221 GN=lipB PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 1.0e-09
Identity = 28/50 (56.00%), Postives = 37/50 (74.00%), Query Frame = 0


Query: 2   DLHWYLRALEEVIIRVLSSTFSINAHRIDGLTGVWAGDQKLEAIGIRVSK 52
           DLHWYLR LE+V+I VL+  F +   R  G TGVWAG +K+ A+GI+VS+
Sbjct: 98  DLHWYLRTLEQVLIEVLAD-FGVQGEREAGFTGVWAGGRKIAALGIKVSR 146

[Close]

BLAST of Bhi11G000969 vs. ExPASy Swiss-Prot
Match: Q31KN6 (Octanoyltransferase OS=Synechococcus elongatus (strain PCC 7942 / FACHB-805) OX=1140 GN=lipB PE=3 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 2.2e-09
Identity = 29/50 (58.00%), Postives = 38/50 (76.00%), Query Frame = 0


Query: 2   DLHWYLRALEEVIIRVLSSTFSINAHRIDGLTGVWAGDQKLEAIGIRVSK 52
           DLHWYLR LE V+I VL++ + +   RI+GLTGVW   +KL AIGI+VS+
Sbjct: 102 DLHWYLRQLEAVLILVLAN-YGLTGERIEGLTGVWVEGKKLAAIGIQVSR 150

[Close]

BLAST of Bhi11G000969 vs. ExPASy Swiss-Prot
Match: Q5N180 (Octanoyltransferase OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 1402/1) OX=269084 GN=lipB PE=3 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 2.2e-09
Identity = 29/50 (58.00%), Postives = 38/50 (76.00%), Query Frame = 0


Query: 2   DLHWYLRALEEVIIRVLSSTFSINAHRIDGLTGVWAGDQKLEAIGIRVSK 52
           DLHWYLR LE V+I VL++ + +   RI+GLTGVW   +KL AIGI+VS+
Sbjct: 102 DLHWYLRQLEAVLILVLAN-YGLTGERIEGLTGVWVEGKKLAAIGIQVSR 150

[Close]

BLAST of Bhi11G000969 vs. NCBI nr
Match: KAA0034631.1 (plastidial lipoyltransferase 2-like [Cucumis melo var. makuwa] >TYK09182.1 plastidial lipoyltransferase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 101.7 bits (252), Expect = 2.0e-18
Identity = 48/51 (94.12%), Postives = 50/51 (98.04%), Query Frame = 0


Query: 1   MDLHWYLRALEEVIIRVLSSTFSINAHRIDGLTGVWAGDQKLEAIGIRVSK 52
           MDLHWYLRALEEV+IRVLSSTFSINAHRIDGLTGVWAG+QKL AIGIRVSK
Sbjct: 349 MDLHWYLRALEEVVIRVLSSTFSINAHRIDGLTGVWAGNQKLAAIGIRVSK 399

[Close]

BLAST of Bhi11G000969 vs. NCBI nr
Match: XP_031742007.1 (octanoyltransferase LIP2p, chloroplastic isoform X3 [Cucumis sativus])

HSP 1 Score: 101.7 bits (252), Expect = 2.0e-18
Identity = 48/51 (94.12%), Postives = 50/51 (98.04%), Query Frame = 0


Query: 1   MDLHWYLRALEEVIIRVLSSTFSINAHRIDGLTGVWAGDQKLEAIGIRVSK 52
           MDLHWYLRALEEV+IRVLSSTFSINAHRIDGLTGVWAG+QKL AIGIRVSK
Sbjct: 158 MDLHWYLRALEEVVIRVLSSTFSINAHRIDGLTGVWAGNQKLAAIGIRVSK 208

[Close]

BLAST of Bhi11G000969 vs. NCBI nr
Match: XP_016900264.1 (PREDICTED: plastidial lipoyltransferase 2-like [Cucumis melo])

HSP 1 Score: 101.7 bits (252), Expect = 2.0e-18
Identity = 48/51 (94.12%), Postives = 50/51 (98.04%), Query Frame = 0


Query: 1   MDLHWYLRALEEVIIRVLSSTFSINAHRIDGLTGVWAGDQKLEAIGIRVSK 52
           MDLHWYLRALEEV+IRVLSSTFSINAHRIDGLTGVWAG+QKL AIGIRVSK
Sbjct: 158 MDLHWYLRALEEVVIRVLSSTFSINAHRIDGLTGVWAGNQKLAAIGIRVSK 208

[Close]

BLAST of Bhi11G000969 vs. NCBI nr
Match: XP_004135053.2 (octanoyltransferase LIP2p, chloroplastic isoform X1 [Cucumis sativus] >KGN52158.1 hypothetical protein Csa_008925 [Cucumis sativus])

HSP 1 Score: 101.7 bits (252), Expect = 2.0e-18
Identity = 48/51 (94.12%), Postives = 50/51 (98.04%), Query Frame = 0


Query: 1   MDLHWYLRALEEVIIRVLSSTFSINAHRIDGLTGVWAGDQKLEAIGIRVSK 52
           MDLHWYLRALEEV+IRVLSSTFSINAHRIDGLTGVWAG+QKL AIGIRVSK
Sbjct: 158 MDLHWYLRALEEVVIRVLSSTFSINAHRIDGLTGVWAGNQKLAAIGIRVSK 208

[Close]

BLAST of Bhi11G000969 vs. NCBI nr
Match: XP_031742006.1 (octanoyltransferase LIP2p, chloroplastic isoform X2 [Cucumis sativus])

HSP 1 Score: 101.7 bits (252), Expect = 2.0e-18
Identity = 48/51 (94.12%), Postives = 50/51 (98.04%), Query Frame = 0


Query: 1   MDLHWYLRALEEVIIRVLSSTFSINAHRIDGLTGVWAGDQKLEAIGIRVSK 52
           MDLHWYLRALEEV+IRVLSSTFSINAHRIDGLTGVWAG+QKL AIGIRVSK
Sbjct: 158 MDLHWYLRALEEVVIRVLSSTFSINAHRIDGLTGVWAGNQKLAAIGIRVSK 208

[Close]

BLAST of Bhi11G000969 vs. ExPASy TrEMBL
Match: A0A5D3CD13 (Lipoyl(octanoyl) transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G001740 PE=3 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 9.5e-19
Identity = 48/51 (94.12%), Postives = 50/51 (98.04%), Query Frame = 0


Query: 1   MDLHWYLRALEEVIIRVLSSTFSINAHRIDGLTGVWAGDQKLEAIGIRVSK 52
           MDLHWYLRALEEV+IRVLSSTFSINAHRIDGLTGVWAG+QKL AIGIRVSK
Sbjct: 349 MDLHWYLRALEEVVIRVLSSTFSINAHRIDGLTGVWAGNQKLAAIGIRVSK 399

[Close]

BLAST of Bhi11G000969 vs. ExPASy TrEMBL
Match: A0A0A0KRC7 (Lipoyl(octanoyl) transferase OS=Cucumis sativus OX=3659 GN=Csa_5G612870 PE=3 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 9.5e-19
Identity = 48/51 (94.12%), Postives = 50/51 (98.04%), Query Frame = 0


Query: 1   MDLHWYLRALEEVIIRVLSSTFSINAHRIDGLTGVWAGDQKLEAIGIRVSK 52
           MDLHWYLRALEEV+IRVLSSTFSINAHRIDGLTGVWAG+QKL AIGIRVSK
Sbjct: 158 MDLHWYLRALEEVVIRVLSSTFSINAHRIDGLTGVWAGNQKLAAIGIRVSK 208

[Close]

BLAST of Bhi11G000969 vs. ExPASy TrEMBL
Match: A0A1S4DWA5 (Lipoyl(octanoyl) transferase OS=Cucumis melo OX=3656 GN=LOC103489357 PE=3 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 9.5e-19
Identity = 48/51 (94.12%), Postives = 50/51 (98.04%), Query Frame = 0


Query: 1   MDLHWYLRALEEVIIRVLSSTFSINAHRIDGLTGVWAGDQKLEAIGIRVSK 52
           MDLHWYLRALEEV+IRVLSSTFSINAHRIDGLTGVWAG+QKL AIGIRVSK
Sbjct: 158 MDLHWYLRALEEVVIRVLSSTFSINAHRIDGLTGVWAGNQKLAAIGIRVSK 208

[Close]

BLAST of Bhi11G000969 vs. ExPASy TrEMBL
Match: A0A6J1D9U7 (Lipoyl(octanoyl) transferase OS=Momordica charantia OX=3673 GN=LOC111018691 PE=3 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 1.6e-18
Identity = 47/51 (92.16%), Postives = 50/51 (98.04%), Query Frame = 0


Query: 1   MDLHWYLRALEEVIIRVLSSTFSINAHRIDGLTGVWAGDQKLEAIGIRVSK 52
           MDLHWYLRALEEV+IRVLSSTFSINAHR+DGLTGVWAG+QKL AIGIRVSK
Sbjct: 153 MDLHWYLRALEEVVIRVLSSTFSINAHRLDGLTGVWAGNQKLAAIGIRVSK 203

[Close]

BLAST of Bhi11G000969 vs. ExPASy TrEMBL
Match: A0A6J1G154 (Lipoyl(octanoyl) transferase OS=Cucurbita moschata OX=3662 GN=LOC111449727 PE=3 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 6.1e-18
Identity = 47/51 (92.16%), Postives = 50/51 (98.04%), Query Frame = 0


Query: 1   MDLHWYLRALEEVIIRVLSSTFSINAHRIDGLTGVWAGDQKLEAIGIRVSK 52
           MDLHWYLRALEEVIIRVLSSTFSINA+R+DGLTGVWAG+QKL AIGIRVSK
Sbjct: 160 MDLHWYLRALEEVIIRVLSSTFSINANRVDGLTGVWAGNQKLAAIGIRVSK 210

The following BLAST results are available for this feature:

BLAST of Bhi11G000969 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G31050.1	5.5e-19	80.39	Biotin/lipoate A/B protein ligase family	[more]
AT1G47578.1	4.6e-18	76.47	Biotin/lipoate A/B protein ligase family	[more]

BLAST of Bhi11G000969 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q948J9	7.7e-18	80.39	Octanoyltransferase LIP2p, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LIP2...	[more]
P0C7R2	6.5e-17	76.47	Octanoyltransferase LIP2p2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LIP...	[more]
Q7ND22	1.0e-09	56.00	Octanoyltransferase OS=Gloeobacter violaceus (strain ATCC 29082 / PCC 7421) OX=2...	[more]
Q31KN6	2.2e-09	58.00	Octanoyltransferase OS=Synechococcus elongatus (strain PCC 7942 / FACHB-805) OX=...	[more]
Q5N180	2.2e-09	58.00	Octanoyltransferase OS=Synechococcus sp. (strain ATCC 27144 / PCC 6301 / SAUG 14...	[more]

BLAST of Bhi11G000969 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0034631.1	2.0e-18	94.12	plastidial lipoyltransferase 2-like [Cucumis melo var. makuwa] >TYK09182.1 plast...	[more]
XP_031742007.1	2.0e-18	94.12	octanoyltransferase LIP2p, chloroplastic isoform X3 [Cucumis sativus]	[more]
XP_016900264.1	2.0e-18	94.12	PREDICTED: plastidial lipoyltransferase 2-like [Cucumis melo]	[more]
XP_004135053.2	2.0e-18	94.12	octanoyltransferase LIP2p, chloroplastic isoform X1 [Cucumis sativus] >KGN52158....	[more]
XP_031742006.1	2.0e-18	94.12	octanoyltransferase LIP2p, chloroplastic isoform X2 [Cucumis sativus]	[more]

BLAST of Bhi11G000969 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CD13	9.5e-19	94.12	Lipoyl(octanoyl) transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca...	[more]
A0A0A0KRC7	9.5e-19	94.12	Lipoyl(octanoyl) transferase OS=Cucumis sativus OX=3659 GN=Csa_5G612870 PE=3 SV=...	[more]
A0A1S4DWA5	9.5e-19	94.12	Lipoyl(octanoyl) transferase OS=Cucumis melo OX=3656 GN=LOC103489357 PE=3 SV=1	[more]
A0A6J1D9U7	1.6e-18	92.16	Lipoyl(octanoyl) transferase OS=Momordica charantia OX=3673 GN=LOC111018691 PE=3...	[more]
A0A6J1G154	6.1e-18	92.16	Lipoyl(octanoyl) transferase OS=Cucurbita moschata OX=3662 GN=LOC111449727 PE=3 ...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.930.10	Bira Bifunctional Protein; Domain 2	coord: 1..51 e-value: 2.7E-13 score: 51.8
None	No IPR available	PANTHER	PTHR10993:SF14	BNAC03G77390D PROTEIN	coord: 1..51
None	No IPR available	PANTHER	PTHR10993	OCTANOYLTRANSFERASE	coord: 1..51
None	No IPR available	SUPERFAMILY	55681	Class II aaRS and biotin synthetases	coord: 1..51
IPR004143	Biotinyl protein ligase (BPL) and lipoyl protein ligase (LPL), catalytic domain	PROSITE	PS51733	BPL_LPL_CATALYTIC	coord: 1..51 score: 16.611155

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi11M000969	Bhi11M000969	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009249	protein lipoylation
biological_process	GO:0006464	cellular protein modification process
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0016874	ligase activity
molecular_function	GO:0033819	lipoyl(octanoyl) transferase activity
molecular_function	GO:0102555	octanoyl transferase activity (acting on glycine-cleavage complex H protein)