Bhi11G000591 (gene) Wax gourd (B227) v1

Overview


Name	Bhi11G000591
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	(+)-gamma-cadinene synthase
Location	chr11: 18971624 .. 18971950 (+)
RNA-Seq Expression	Bhi11G000591
Synteny	Bhi11G000591

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTTCTCAAATTTCAAATTCTCCTACTTCAATTATGAAAGCCAATGACATTCCTGATACTAAGCGTTCTATTGCAAATTTTCATCCTAGCATTTGGACTAATCATTTCCTTTCATCTACCTTTGATGATGCATTGGTAAAACTAAATTCCAATACTTTTCTATTTTGAATAATTTTAAATTTTTATTCTTTTAATTTCAGAAAGTAAACGATGGAACGAAAGAACAAGCTAGAAAGTTGAAAGAAGAAATACGGATGATGGTAGTTGTTTTAGTGGAAAACCCATTGGAGAAGCTAACATTGGTTGATTCAATCCAACGACTAG

mRNA sequence

ATGTCTTCTCAAATTTCAAATTCTCCTACTTCAATTATGAAAGCCAATGACATTCCTGATACTAAGCGTTCTATTGCAAATTTTCATCCTAGCATTTGGACTAATCATTTCCTTTCATCTACCTTTGATGATGCATTGAAAGTAAACGATGGAACGAAAGAACAAGCTAGAAAGTTGAAAGAAGAAATACGGATGATGGTAGTTGTTTTAGTGGAAAACCCATTGGAGAAGCTAACATTGGTTGATTCAATCCAACGACTAG

Coding sequence (CDS)

ATGTCTTCTCAAATTTCAAATTCTCCTACTTCAATTATGAAAGCCAATGACATTCCTGATACTAAGCGTTCTATTGCAAATTTTCATCCTAGCATTTGGACTAATCATTTCCTTTCATCTACCTTTGATGATGCATTGAAAGTAAACGATGGAACGAAAGAACAAGCTAGAAAGTTGAAAGAAGAAATACGGATGATGGTAGTTGTTTTAGTGGAAAACCCATTGGAGAAGCTAACATTGGTTGATTCAATCCAACGACTAG

Protein sequence

MSSQISNSPTSIMKANDIPDTKRSIANFHPSIWTNHFLSSTFDDALKVNDGTKEQARKLKEEIRMMVVVLVENPLEKLTLVDSIQRL

Homology

[Close]

BLAST of Bhi11G000591 vs. ExPASy Swiss-Prot
Match: B2KSJ5 ((+)-gamma-cadinene synthase OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 1.3e-28
Identity = 63/87 (72.41%), Postives = 72/87 (82.76%), Query Frame = 0


Query: 1  MSSQISNSPTSIMKANDIPDTKRSIANFHPSIWTNHFLSSTFDDALKVNDGTKEQARKLK 60
          MSSQ+SN P SIMK NDIPD KRS+ANFHP+IW  HFLS TFDDALK+++G KE+  KLK
Sbjct: 1  MSSQVSNFPASIMKTNDIPDVKRSLANFHPNIWKEHFLSFTFDDALKIDEGMKERTEKLK 60

Query: 61 EEIRMMVVVLVENPLEKLTLVDSIQRL 88
          EEIRMM++  VEN L KL LVDSIQRL
Sbjct: 61 EEIRMMMIAYVENQLIKLNLVDSIQRL 87

[Close]

BLAST of Bhi11G000591 vs. ExPASy Swiss-Prot
Match: Q6Q3H3 ((-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 1.5e-08
Identity = 30/69 (43.48%), Postives = 45/69 (65.22%), Query Frame = 0


Query: 19 PDTKRSIANFHPSIWTNHFLSSTFDDALKVNDGTKEQARKLKEEIRMMVVVLVENPLEKL 78
          P+  R  ANFHPSIW +HFLS    +    +D  K+  ++LKEE+R M++   ++  +KL
Sbjct: 18 PEVGRRCANFHPSIWGDHFLSYA-SEFTNTDDHLKQHVQQLKEEVRKMLMAADDDSAQKL 77

Query: 79 TLVDSIQRL 88
           L+D+IQRL
Sbjct: 78 LLIDAIQRL 85

[Close]

BLAST of Bhi11G000591 vs. ExPASy Swiss-Prot
Match: Q94JS8 ((E)-beta-farnesene synthase OS=Citrus junos OX=135197 PE=1 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 7.2e-08
Identity = 28/68 (41.18%), Postives = 43/68 (63.24%), Query Frame = 0


Query: 20 DTKRSIANFHPSIWTNHFLSSTFDDALKVNDGTKEQARKLKEEIRMMVVVLVENPLEKLT 79
          +T R IA+FHP++W NHFL S   D   ++  T+EQ   LK+E+R M+        +KL 
Sbjct: 18 ETVRPIADFHPTLWGNHFLKSA-ADVETIDAATQEQHAALKQEVRRMITTTANKLAQKLH 77

Query: 80 LVDSIQRL 88
          ++D++QRL
Sbjct: 78 MIDAVQRL 84

[Close]

BLAST of Bhi11G000591 vs. ExPASy Swiss-Prot
Match: Q5SBP7 (Selinene synthase OS=Ocimum basilicum OX=39350 GN=SES PE=1 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 3.6e-07
Identity = 32/83 (38.55%), Postives = 52/83 (62.65%), Query Frame = 0


Query: 5  ISNSPTSIMKANDIPDTKRSIANFHPSIWTNHFLSSTFDDALKVNDGTKEQARKLKEEIR 64
          +S +PTS  K +D+ + ++S A +H S+W NHFLS T  D  ++    KEQ  KLKE+++
Sbjct: 6  VSAAPTS-PKNSDVEEIRKS-ATYHSSVWGNHFLSYT-SDVTEITAAEKEQLEKLKEKVK 65

Query: 65 MMVVVLVENPLEKLTLVDSIQRL 88
           ++    +    K+ L+D+IQRL
Sbjct: 66 NLLAQTPDESTGKMELIDAIQRL 85

[Close]

BLAST of Bhi11G000591 vs. ExPASy Swiss-Prot
Match: B9S9Z3 (Alpha-copaene synthase OS=Ricinus communis OX=3988 GN=TPS1 PE=1 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 3.6e-07
Identity = 32/88 (36.36%), Postives = 52/88 (59.09%), Query Frame = 0


Query: 1  MSSQISNSPTSIMKANDIPDTKRSIANFHPSIWTNHFLSSTFDDALKVNDGT-KEQARKL 60
          MS Q+S  P      N     KR  + +HP+IW +HFL++      K+ DG+ ++Q   L
Sbjct: 1  MSLQVSAVPIKTSTQNATSAVKRHSSTYHPTIWGDHFLANLSHS--KIIDGSIEQQFEGL 60

Query: 61 KEEIRMMVVVLVENPLEKLTLVDSIQRL 88
          K+++R M++ L   P +KL L+D++QRL
Sbjct: 61 KQKVRKMIIDLNNEPCKKLGLIDAVQRL 86

[Close]

BLAST of Bhi11G000591 vs. NCBI nr
Match: XP_038905757.1 ((+)-gamma-cadinene synthase-like [Benincasa hispida])

HSP 1 Score: 139.4 bits (350), Expect = 1.4e-29
Identity = 71/87 (81.61%), Postives = 78/87 (89.66%), Query Frame = 0


Query: 1  MSSQISNSPTSIMKANDIPDTKRSIANFHPSIWTNHFLSSTFDDALKVNDGTKEQARKLK 60
          MSS+ISN PTS+MK NDIPDTKRS ANFHP+IWT+HFLS T DDALK++DG KEQ +KLK
Sbjct: 1  MSSKISNFPTSVMKTNDIPDTKRSTANFHPNIWTDHFLSFTSDDALKLDDGMKEQVKKLK 60

Query: 61 EEIRMMVVVLVENPLEKLTLVDSIQRL 88
          EEIRMMVVV VENPLEKL LVDSIQRL
Sbjct: 61 EEIRMMVVVYVENPLEKLKLVDSIQRL 87

[Close]

BLAST of Bhi11G000591 vs. NCBI nr
Match: NP_001284382.1 ((+)-gamma-cadinene synthase [Cucumis melo] >B2KSJ5.1 RecName: Full=(+)-gamma-cadinene synthase; Short=CmTpsNY; AltName: Full=(+)-delta-cadinene synthase [Cucumis melo] >ABX83200.1 terpene synthase [Cucumis melo])

HSP 1 Score: 126.7 bits (317), Expect = 9.7e-26
Identity = 63/87 (72.41%), Postives = 72/87 (82.76%), Query Frame = 0


Query: 1  MSSQISNSPTSIMKANDIPDTKRSIANFHPSIWTNHFLSSTFDDALKVNDGTKEQARKLK 60
          MSSQ+SN P SIMK NDIPD KRS+ANFHP+IW  HFLS TFDDALK+++G KE+  KLK
Sbjct: 1  MSSQVSNFPASIMKTNDIPDVKRSLANFHPNIWKEHFLSFTFDDALKIDEGMKERTEKLK 60

Query: 61 EEIRMMVVVLVENPLEKLTLVDSIQRL 88
          EEIRMM++  VEN L KL LVDSIQRL
Sbjct: 61 EEIRMMMIAYVENQLIKLNLVDSIQRL 87

[Close]

BLAST of Bhi11G000591 vs. NCBI nr
Match: NP_001315388.1 ((+)-gamma-cadinene synthase-like [Cucumis melo] >AJD19682.1 sesquiterpene synthase Tps1 [Cucumis melo])

HSP 1 Score: 125.9 bits (315), Expect = 1.7e-25
Identity = 64/87 (73.56%), Postives = 72/87 (82.76%), Query Frame = 0


Query: 1  MSSQISNSPTSIMKANDIPDTKRSIANFHPSIWTNHFLSSTFDDALKVNDGTKEQARKLK 60
          MSSQ+SN PTSIMK NDIPD KRS+A FHP+IW  HFLS TFDDALKV++G KE+  KLK
Sbjct: 1  MSSQVSNFPTSIMKTNDIPDVKRSLAIFHPNIWKEHFLSFTFDDALKVDEGMKERTEKLK 60

Query: 61 EEIRMMVVVLVENPLEKLTLVDSIQRL 88
          EEIRMM++  VEN L KL LVDSIQRL
Sbjct: 61 EEIRMMMIAYVENQLVKLNLVDSIQRL 87

[Close]

BLAST of Bhi11G000591 vs. NCBI nr
Match: XP_038903601.1 ((+)-gamma-cadinene synthase-like [Benincasa hispida])

HSP 1 Score: 124.8 bits (312), Expect = 3.7e-25
Identity = 64/75 (85.33%), Postives = 68/75 (90.67%), Query Frame = 0


Query: 13 MKANDIPDTKRSIANFHPSIWTNHFLSSTFDDALKVNDGTKEQARKLKEEIRMMVVVLVE 72
          MKANDIPDTKRSIANFHPSIWT+HFLS TFDDALKV++  KEQA  LKEEIRMMVVV V+
Sbjct: 1  MKANDIPDTKRSIANFHPSIWTDHFLSVTFDDALKVDNEMKEQAENLKEEIRMMVVVSVK 60

Query: 73 NPLEKLTLVDSIQRL 88
          NPLEKL LVDSIQRL
Sbjct: 61 NPLEKLKLVDSIQRL 75

[Close]

BLAST of Bhi11G000591 vs. NCBI nr
Match: NP_001292628.1 ((+)-gamma-cadinene synthase [Cucumis sativus] >AAU05952.1 beta-caryophyllene synthase [Cucumis sativus])

HSP 1 Score: 117.5 bits (293), Expect = 5.9e-23
Identity = 60/82 (73.17%), Postives = 67/82 (81.71%), Query Frame = 0


Query: 6  SNSPTSIMKANDIPDTKRSIANFHPSIWTNHFLSSTFDDALKVNDGTKEQARKLKEEIRM 65
          S+ P SIMK NDI DTKRS+ANFHP+IW  HFLS TFDDALKV+ G KE+  KLKEEIRM
Sbjct: 3  SHFPASIMKTNDIYDTKRSLANFHPTIWKEHFLSFTFDDALKVDGGMKERIEKLKEEIRM 62

Query: 66 MVVVLVENPLEKLTLVDSIQRL 88
          MV+  V+NPL KL LVDSIQRL
Sbjct: 63 MVIASVQNPLVKLNLVDSIQRL 84

[Close]

BLAST of Bhi11G000591 vs. ExPASy TrEMBL
Match: A0A1S4DZR2 ((+)-gamma-cadinene synthase-like OS=Cucumis melo OX=3656 GN=LOC103493390 PE=4 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 7.2e-27
Identity = 65/87 (74.71%), Postives = 73/87 (83.91%), Query Frame = 0


Query: 1  MSSQISNSPTSIMKANDIPDTKRSIANFHPSIWTNHFLSSTFDDALKVNDGTKEQARKLK 60
          MSSQ+SN PTSIMK NDIPD KRS+ANFHP+IW  HFLS TFDDALKV++G KE+  KLK
Sbjct: 1  MSSQVSNFPTSIMKTNDIPDVKRSLANFHPNIWKEHFLSFTFDDALKVDEGMKERTEKLK 60

Query: 61 EEIRMMVVVLVENPLEKLTLVDSIQRL 88
          EEIRMM++  VEN L KL LVDSIQRL
Sbjct: 61 EEIRMMMIAYVENQLVKLNLVDSIQRL 87

[Close]

BLAST of Bhi11G000591 vs. ExPASy TrEMBL
Match: A0A1S2X8Q6 ((+)-gamma-cadinene synthase OS=Cucumis melo OX=3656 GN=TPS PE=4 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 4.7e-26
Identity = 63/87 (72.41%), Postives = 72/87 (82.76%), Query Frame = 0


Query: 1  MSSQISNSPTSIMKANDIPDTKRSIANFHPSIWTNHFLSSTFDDALKVNDGTKEQARKLK 60
          MSSQ+SN P SIMK NDIPD KRS+ANFHP+IW  HFLS TFDDALK+++G KE+  KLK
Sbjct: 1  MSSQVSNFPASIMKTNDIPDVKRSLANFHPNIWKEHFLSFTFDDALKIDEGMKERTEKLK 60

Query: 61 EEIRMMVVVLVENPLEKLTLVDSIQRL 88
          EEIRMM++  VEN L KL LVDSIQRL
Sbjct: 61 EEIRMMMIAYVENQLIKLNLVDSIQRL 87

[Close]

BLAST of Bhi11G000591 vs. ExPASy TrEMBL
Match: A0A0B4VEV4 (Sesquiterpene synthase Tps1 OS=Cucumis melo OX=3656 PE=2 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 8.0e-26
Identity = 64/87 (73.56%), Postives = 72/87 (82.76%), Query Frame = 0


Query: 1  MSSQISNSPTSIMKANDIPDTKRSIANFHPSIWTNHFLSSTFDDALKVNDGTKEQARKLK 60
          MSSQ+SN PTSIMK NDIPD KRS+A FHP+IW  HFLS TFDDALKV++G KE+  KLK
Sbjct: 1  MSSQVSNFPTSIMKTNDIPDVKRSLAIFHPNIWKEHFLSFTFDDALKVDEGMKERTEKLK 60

Query: 61 EEIRMMVVVLVENPLEKLTLVDSIQRL 88
          EEIRMM++  VEN L KL LVDSIQRL
Sbjct: 61 EEIRMMMIAYVENQLVKLNLVDSIQRL 87

[Close]

BLAST of Bhi11G000591 vs. ExPASy TrEMBL
Match: Q66PX8 (Beta-caryophyllene synthase OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 2.8e-23
Identity = 60/82 (73.17%), Postives = 67/82 (81.71%), Query Frame = 0


Query: 6  SNSPTSIMKANDIPDTKRSIANFHPSIWTNHFLSSTFDDALKVNDGTKEQARKLKEEIRM 65
          S+ P SIMK NDI DTKRS+ANFHP+IW  HFLS TFDDALKV+ G KE+  KLKEEIRM
Sbjct: 3  SHFPASIMKTNDIYDTKRSLANFHPTIWKEHFLSFTFDDALKVDGGMKERIEKLKEEIRM 62

Query: 66 MVVVLVENPLEKLTLVDSIQRL 88
          MV+  V+NPL KL LVDSIQRL
Sbjct: 63 MVIASVQNPLVKLNLVDSIQRL 84

[Close]

BLAST of Bhi11G000591 vs. ExPASy TrEMBL
Match: A0A0A0L579 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G097040 PE=4 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 2.8e-23
Identity = 60/82 (73.17%), Postives = 67/82 (81.71%), Query Frame = 0


Query: 6  SNSPTSIMKANDIPDTKRSIANFHPSIWTNHFLSSTFDDALKVNDGTKEQARKLKEEIRM 65
          S+ P SIMK NDI DTKRS+ANFHP+IW  HFLS TFDDALKV+ G KE+  KLKEEIRM
Sbjct: 3  SHFPASIMKTNDIYDTKRSLANFHPTIWKEHFLSFTFDDALKVDGGMKERIEKLKEEIRM 62

Query: 66 MVVVLVENPLEKLTLVDSIQRL 88
          MV+  V+NPL KL LVDSIQRL
Sbjct: 63 MVIASVQNPLVKLNLVDSIQRL 84

The following BLAST results are available for this feature:

BLAST of Bhi11G000591 vs.


Match Name	E-value	Identity	Description

BLAST of Bhi11G000591 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B2KSJ5	1.3e-28	72.41	(+)-gamma-cadinene synthase OS=Cucumis melo OX=3656 PE=1 SV=1	[more]
Q6Q3H3	1.5e-08	43.48	(-)-germacrene D synthase OS=Vitis vinifera OX=29760 GN=VIT_19s0014g04930 PE=1 S...	[more]
Q94JS8	7.2e-08	41.18	(E)-beta-farnesene synthase OS=Citrus junos OX=135197 PE=1 SV=1	[more]
Q5SBP7	3.6e-07	38.55	Selinene synthase OS=Ocimum basilicum OX=39350 GN=SES PE=1 SV=1	[more]
B9S9Z3	3.6e-07	36.36	Alpha-copaene synthase OS=Ricinus communis OX=3988 GN=TPS1 PE=1 SV=1	[more]

BLAST of Bhi11G000591 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038905757.1	1.4e-29	81.61	(+)-gamma-cadinene synthase-like [Benincasa hispida]	[more]
NP_001284382.1	9.7e-26	72.41	(+)-gamma-cadinene synthase [Cucumis melo] >B2KSJ5.1 RecName: Full=(+)-gamma-cad...	[more]
NP_001315388.1	1.7e-25	73.56	(+)-gamma-cadinene synthase-like [Cucumis melo] >AJD19682.1 sesquiterpene syntha...	[more]
XP_038903601.1	3.7e-25	85.33	(+)-gamma-cadinene synthase-like [Benincasa hispida]	[more]
NP_001292628.1	5.9e-23	73.17	(+)-gamma-cadinene synthase [Cucumis sativus] >AAU05952.1 beta-caryophyllene syn...	[more]

BLAST of Bhi11G000591 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S4DZR2	7.2e-27	74.71	(+)-gamma-cadinene synthase-like OS=Cucumis melo OX=3656 GN=LOC103493390 PE=4 SV...	[more]
A0A1S2X8Q6	4.7e-26	72.41	(+)-gamma-cadinene synthase OS=Cucumis melo OX=3656 GN=TPS PE=4 SV=1	[more]
A0A0B4VEV4	8.0e-26	73.56	Sesquiterpene synthase Tps1 OS=Cucumis melo OX=3656 PE=2 SV=1	[more]
Q66PX8	2.8e-23	73.17	Beta-caryophyllene synthase OS=Cucumis sativus OX=3659 PE=2 SV=1	[more]
A0A0A0L579	2.8e-23	73.17	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G097040 PE=4 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 42..62
IPR008949	Isoprenoid synthase domain superfamily	GENE3D	1.10.600.10	Farnesyl Diphosphate Synthase	coord: 26..86 e-value: 1.1E-11 score: 46.2
IPR008930	Terpenoid cyclases/protein prenyltransferase alpha-alpha toroid	SUPERFAMILY	48239	Terpenoid cyclases/Protein prenyltransferases	coord: 29..87

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi11M000591	Bhi11M000591	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016102	diterpenoid biosynthetic process
biological_process	GO:0045338	farnesyl diphosphate metabolic process
biological_process	GO:0051762	sesquiterpene biosynthetic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0047461	(+)-delta-cadinene synthase activity
molecular_function	GO:0000287	magnesium ion binding