Bhi11G000428 (gene) Wax gourd (B227) v1

Overview


Name	Bhi11G000428
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	cytochrome P450 CYP736A12-like
Location	chr11: 12850791 .. 12851474 (+)
RNA-Seq Expression	Bhi11G000428
Synteny	Bhi11G000428

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCGTAAAATATGTTCATTTCAATTGCTTACATCTCTCAAAATCGACTCTTTTTCATCAATTCGAAGGCAAGAACTTGGTTTGTTGATTGATCGTCTTCGAGAGGCTGCGAAAAATCATAGTATTGTGGATCTTAATTCTCAAGTCTCTTCTCTTATTGCTGGTGTGATTTGTGTAATGTTGTTTGGGAAGAAATTTGTTGATGAGGAGTTTATGGCTGCAATACATGAAGTCACTTTCTTGGCTGGTGCTCTTAACTTGGGGGATTTTATTCCTTTTATTGCCTTTCTTGATCTTCAAGGATTAGGTCGTCGTTCCAAAGCTATTAGTAAGGCCTTTGATGGATTTTTAAACACGATTATCGAGCAACATGTCGAGTTTAAGGACCAAAATAACACTAAGAGTGGGCTTTTTGTGGATGTTATGTTGGATCTCATGAGGTCAGAAAAAACAAAGTGCCAAATTGATCGTACGAGTATCAAAGGCGTAATGTTTGTAAGTATACTTTAAAATTTCATTCAACTAAAGTTGGTAATTCGTTAAAGGTCCCATGCTCAAACGATGAAAATTCAAGTATTGAGTACTTACTCTTCTTATTGTCTATGAACTTTTTAATTACAACTTTTCAATAGAATAATTTATTTTTTAAAAAATAATCCATGGGAACCTTGGCCTAGTCCATAA

mRNA sequence

ATGCGTAAAATATGTTCATTTCAATTGCTTACATCTCTCAAAATCGACTCTTTTTCATCAATTCGAAGGCAAGAACTTGGTTTGTTGATTGATCGTCTTCGAGAGGCTGCGAAAAATCATAGTATTGTGGATCTTAATTCTCAAGTCTCTTCTCTTATTGCTGGTGTGATTTGTGTAATGTTGTTTGGGAAGAAATTTGTTGATGAGGAGTTTATGGCTGCAATACATGAAGTCACTTTCTTGGCTGGTGCTCTTAACTTGGGGGATTTTATTCCTTTTATTGCCTTTCTTGATCTTCAAGGATTAGGTCGTCGTTCCAAAGCTATTAGTAAGGCCTTTGATGGATTTTTAAACACGATTATCGAGCAACATGTCGAGTTTAAGGACCAAAATAACACTAAGAGTGGGCTTTTTGTGGATGTTATGTTGGATCTCATGAGGTCAGAAAAAACAAAGTGCCAAATTGATCGTACGAGTATCAAAGGCGTAATGTTTGTAATTGGTAATTCGTTAAAGGTCCCATGCTCAAACGATGAAAATTCAAGTATTGAGTACTTACTCTTCTTATTAATAATTTATTTTTTAAAAAATAATCCATGGGAACCTTGGCCTAGTCCATAA

Coding sequence (CDS)

ATGCGTAAAATATGTTCATTTCAATTGCTTACATCTCTCAAAATCGACTCTTTTTCATCAATTCGAAGGCAAGAACTTGGTTTGTTGATTGATCGTCTTCGAGAGGCTGCGAAAAATCATAGTATTGTGGATCTTAATTCTCAAGTCTCTTCTCTTATTGCTGGTGTGATTTGTGTAATGTTGTTTGGGAAGAAATTTGTTGATGAGGAGTTTATGGCTGCAATACATGAAGTCACTTTCTTGGCTGGTGCTCTTAACTTGGGGGATTTTATTCCTTTTATTGCCTTTCTTGATCTTCAAGGATTAGGTCGTCGTTCCAAAGCTATTAGTAAGGCCTTTGATGGATTTTTAAACACGATTATCGAGCAACATGTCGAGTTTAAGGACCAAAATAACACTAAGAGTGGGCTTTTTGTGGATGTTATGTTGGATCTCATGAGGTCAGAAAAAACAAAGTGCCAAATTGATCGTACGAGTATCAAAGGCGTAATGTTTGTAATTGGTAATTCGTTAAAGGTCCCATGCTCAAACGATGAAAATTCAAGTATTGAGTACTTACTCTTCTTATTAATAATTTATTTTTTAAAAAATAATCCATGGGAACCTTGGCCTAGTCCATAA

Protein sequence

MRKICSFQLLTSLKIDSFSSIRRQELGLLIDRLREAAKNHSIVDLNSQVSSLIAGVICVMLFGKKFVDEEFMAAIHEVTFLAGALNLGDFIPFIAFLDLQGLGRRSKAISKAFDGFLNTIIEQHVEFKDQNNTKSGLFVDVMLDLMRSEKTKCQIDRTSIKGVMFVIGNSLKVPCSNDENSSIEYLLFLLIIYFLKNNPWEPWPSP

Homology

[Close]

BLAST of Bhi11G000428 vs. TAIR 10
Match: AT5G24960.1 (cytochrome P450, family 71, subfamily A, polypeptide 14 )

HSP 1 Score: 90.9 bits (224), Expect = 1.3e-18
Identity = 51/176 (28.98%), Postives = 102/176 (57.95%), Query Frame = 0


Query: 1   MRKICSFQLLTSLKIDSFSSIRRQELGLLIDRLREAAKNHSIVDLNSQVSSLIAGVICVM 60
           ++ +C   LL   K+ SF  +R +E+  +++R+ +A+ + S ++L+  + +L + V   +
Sbjct: 128 IKSVCIVNLLNKKKVQSFEKVREEEISEMMERVEKASSDSSPLNLSELLLTLTSDVTSRV 187

Query: 61  LFGKKFVDEE----FMAAIHEVTFLAGALNLGDFIPFIAFLD-LQGLGRRSKAISKAFDG 120
             G+K+  EE    F   + ++T L G   +G++IP +A++D L+G+  +++ +SKAF  
Sbjct: 188 SLGRKYSKEESMSDFKIQMRKITELVGGFPVGEYIPCLAWIDKLRGVDEKAEEVSKAFGD 247

Query: 121 FLNTIIEQHVEFKDQNNTKSGLFVDVMLDLMRSEKTKCQIDRTSIKGV---MFVIG 169
            +  ++++H++  D+       FVDV+L L R E+   QI R+ IK +   MF+ G
Sbjct: 248 LMEKVLQEHLDATDKPTLD---FVDVLLSLERHERNGVQIRRSDIKFLILDMFLAG 300

[Close]

BLAST of Bhi11G000428 vs. TAIR 10
Match: AT5G06900.1 (cytochrome P450, family 93, subfamily D, polypeptide 1 )

HSP 1 Score: 86.7 bits (213), Expect = 2.4e-17
Identity = 55/174 (31.61%), Postives = 92/174 (52.87%), Query Frame = 0


Query: 1   MRKICSFQLLTSLKIDSFSSIRRQELGLLIDRLREAAKNHSIVDLNSQVSSLIAGVICVM 60
           M++IC  +L +S  +DSF S+R +EL  L+ R+ + A+    V+L  Q+  L + +I  M
Sbjct: 129 MKRICMVELFSSRALDSFVSVRSEELKKLLIRVLKKAEAEESVNLGEQLKELTSNIITRM 188

Query: 61  LFGKKFVD-------EEFMAAIHEVTFLAGALNLGDFIPFIAFLDLQGLGRRSKAISKAF 120
           +F K   D       EE +  + E+  LAG  N+ +   F+  LDLQGL +R K     +
Sbjct: 189 MFRKMQSDSDGGEKSEEVIKMVVELNELAGFFNVSETFWFLKRLDLQGLKKRLKNARDKY 248

Query: 121 DGFLNTIIEQHVEFKDQNNTKSGLFVDVMLDLMRSEKTKCQIDRTSIKGVMFVI 168
           D  +  I+E+H E   +N T     +DV+LD+   +  + ++ R +IK  +  I
Sbjct: 249 DVIIERIMEEH-ESSKKNATGERNMLDVLLDIYEDKNAEMKLTRENIKAFIMNI 301

[Close]

BLAST of Bhi11G000428 vs. TAIR 10
Match: AT2G30770.1 (cytochrome P450, family 71, subfamily A, polypeptide 13 )

HSP 1 Score: 82.8 bits (203), Expect = 3.5e-16
Identity = 45/178 (25.28%), Postives = 99/178 (55.62%), Query Frame = 0


Query: 1   MRKICSFQLLTSLKIDSFSSIRRQELGLLIDRLREAAKNHSIVDLNSQVSSLIAGVICVM 60
           M+ +C   LLT+  ++SF  +R  E+  +I++L +A+ + S  +L+    +L + V   +
Sbjct: 134 MKSVCILNLLTNKMVESFEKVREDEVNAMIEKLEKASSSSSSENLSELFITLPSDVTSRV 193

Query: 61  LFGKKFVDEE----FMAAIHEVTFLAGALNLGDFIPFIAFLD-LQGLGRRSKAISKAFDG 120
             G+K  ++E        + ++  L G   +G+++P +A++D ++G   + K +S+ F  
Sbjct: 194 ALGRKHSEDETARDLKKRVRQIMELLGEFPIGEYVPILAWIDGIRGFNNKIKEVSRGFSD 253

Query: 121 FLNTIIEQHVEFKDQNNTKSGLFVDVMLDLMRSEKTKCQIDRTSIKGV---MFVIGNS 171
            ++ ++++H+E    +N K+  FVD++L + + + +  Q+ R  IK +   MF+ G S
Sbjct: 254 LMDKVVQEHLE---ASNDKAD-FVDILLSIEKDKNSGFQVQRNDIKFMILDMFIGGTS 307

[Close]

BLAST of Bhi11G000428 vs. TAIR 10
Match: AT4G20240.1 (cytochrome P450, family 71, subfamily A, polypeptide 27 )

HSP 1 Score: 77.8 bits (190), Expect = 1.1e-14
Identity = 43/165 (26.06%), Postives = 94/165 (56.97%), Query Frame = 0


Query: 1   MRKICSFQLLTSLKIDSFSSIRRQELGLLIDRLREAAKNHSIVDLNSQVSSLIAGVICVM 60
           M+ +    LL +  + SF ++R +E+ ++ ++L EA+ + S V+L+  + +L   +IC +
Sbjct: 128 MKSLGVVHLLNNKMVRSFENLREEEIKVMTEKLEEASSSSSSVNLSKLLMTLTNDIICRI 187

Query: 61  LFGKKFVDEEFMAAIHEVTFLA----GALNLGDFIPFIAFLD-LQGLGRRSKAISKAFDG 120
             G+K+ +EE    I  +   +    G    GDFIP +A++D + G+  + K I+   D 
Sbjct: 188 TLGRKYNEEEGGIDIKNLVMTSSEFFGKFFFGDFIPSLAWIDWISGIDDKMKDINNKLDC 247

Query: 121 FLNTIIEQHVEFKDQNNTKSGLFVDVMLDLMRSEKTKCQIDRTSI 161
           FL++++++HV   D ++ +   F+D++L + + +  + + DR+ +
Sbjct: 248 FLDSMVQEHV---DADHKEPSDFIDMLLLIQKDKTKRFKFDRSDL 289

[Close]

BLAST of Bhi11G000428 vs. TAIR 10
Match: AT5G07990.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 73.6 bits (179), Expect = 2.1e-13
Identity = 42/141 (29.79%), Postives = 77/141 (54.61%), Query Frame = 0


Query: 1   MRKICSFQLLTSLKIDSFSSIRRQELGLLIDRLREAAKNHSIVDLNSQVSSLIAGVICVM 60
           +RKI S  L ++  ++ F  +R++E+G L   L         V+L   V+  +   +   
Sbjct: 128 LRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKP--VNLGQLVNMCVVNALGRE 187

Query: 61  LFGKKFVD-------EEFMAAIHEVTFLAGALNLGDFIPFIAFLDLQGLGRRSKAISKAF 120
           + G++          +EF + + E+  LAG  N+GDF+P + +LDLQG+  + K + K F
Sbjct: 188 MIGRRLFGADADHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGKMKRLHKRF 247

Query: 121 DGFLNTIIEQH-VEFKDQNNT 134
           D FL++I+++H +  +DQ +T
Sbjct: 248 DAFLSSILKEHEMNGQDQKHT 266

[Close]

BLAST of Bhi11G000428 vs. ExPASy Swiss-Prot
Match: A0A068Q5V6 (Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1)

HSP 1 Score: 137.1 bits (344), Expect = 2.2e-31
Identity = 76/169 (44.97%), Postives = 111/169 (65.68%), Query Frame = 0


Query: 2   RKICSFQLLTSLKIDSFSSIRRQELGLLIDRLREAAKNHSI-VDLNSQVSSLIAGVICVM 61
           RK+C+ +LL++ KI+SF S+RR+E+ L ++ +R AA N  + VDL+ +VSSL   + C M
Sbjct: 124 RKMCTIELLSNHKINSFKSMRREEVSLCVESIRAAANNRGVAVDLSDKVSSLSVDMSCRM 183

Query: 62  LFGKKFVDEE-----FMAAIHEVTFLAGALNLGDFIPFIAFLDLQGLGRRSKAISKAFDG 121
           + GKK+ DEE     F + + E   LA A NLGD+I FIA LDLQG  +R K+++KAFD 
Sbjct: 184 VLGKKYRDEEFDERGFKSVVREAIQLASAPNLGDYIRFIAPLDLQGFTKRMKSVNKAFDN 243

Query: 122 FLNTIIEQHVEFKDQNNTKSGLFVDVMLDLMRSEKTKCQIDRTSIKGVM 165
               IIE+H++  D   T    FVDVM+  M SE+++ +I+R  IK +M
Sbjct: 244 LFEKIIEEHLQPNDGERTMD--FVDVMVGFMGSEESEYRIERPHIKAIM 290

[Close]

BLAST of Bhi11G000428 vs. ExPASy Swiss-Prot
Match: H2DH18 (Cytochrome P450 CYP736A12 OS=Panax ginseng OX=4054 PE=2 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 2.5e-22
Identity = 68/197 (34.52%), Postives = 111/197 (56.35%), Query Frame = 0


Query: 1   MRKICSFQLLTSLKIDSFSSIRRQELGLLIDRLREAAKNHSIVDLNSQVSSLIAGVICVM 60
           +RK    +LLT  KI+SF  +RR+ELG ++  ++EA+  + +VDL+++V+++I  +   +
Sbjct: 128 IRKFVVLELLTPAKINSFVGMRREELGTVVKSIKEASAANEVVDLSAKVANIIENMTYRL 187

Query: 61  LFGKKFVDE-EFMAAIHEVTFLAGALNLGDFIPFIAFLDLQGLGRRSKAISKAFDGFLNT 120
           L G+   D  +    ++E   LAG  N+ DF+PF+  LD+QGL R+ K   K  D  L  
Sbjct: 188 LLGRTKDDRYDLKGIMNEALTLAGRFNIADFVPFLGPLDIQGLTRQFKDTGKRLDKILEF 247

Query: 121 IIEQHVEFKDQNNTKSGLFVDVMLDLMRS-----EKTKCQIDRTSIKGVMFVIGNSLKVP 180
           II++H E    N   SG F+D ML L        ++    IDR+ IK +M  I ++    
Sbjct: 248 IIDEH-EQNSSNGNASGDFIDDMLSLKNKPSNTHDELSKVIDRSVIKAIMIDIISA---- 307

Query: 181 CSNDENSSIEYLLFLLI 192
             +  ++SIE++L  LI
Sbjct: 308 AIDTSDTSIEWILTELI 319

[Close]

BLAST of Bhi11G000428 vs. ExPASy Swiss-Prot
Match: Q50EK4 (Cytochrome P450 750A1 OS=Pinus taeda OX=3352 GN=CYP750A1 PE=2 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 3.6e-21
Identity = 57/179 (31.84%), Postives = 103/179 (57.54%), Query Frame = 0


Query: 1   MRKICSFQLLTSLKIDSFSSIRRQELGLLIDRLREAAKNHSI-VDLNSQVSSLIAGVICV 60
           MRKIC  +LLTS +I+SF  +R++EL  +I  + E +++  I V+++  +S+ +A ++  
Sbjct: 137 MRKICVLELLTSKRIESFKHVRQEELSAMIHSIWEESESGRIAVNVSKAISTSLANILWR 196

Query: 61  MLFGKKFVDEE-------FMAAIHEVTFLAGALNLGDFIPFIAFLDLQGLGRRSKAISKA 120
           +L  KKF D +       F   + EV+   G+LN+GDFIP++  LDLQG+ R  K  +  
Sbjct: 197 ILARKKFSDNDLGADGKGFADLVVEVSIAVGSLNIGDFIPYLDCLDLQGIKRALKKANAR 256

Query: 121 FDGFLNTIIEQHVEFKDQNNTKSGL------FVDVMLDLMRSEKTKCQIDRTSIKGVMF 166
           FD F   +I++H+      N ++         +DV+L++ +++ T  ++ R  IK + +
Sbjct: 257 FDAFAEKMIDEHINASTIRNGEADAGCHVKDIIDVLLEMAKNDNTGAKVTREIIKAITY 315

[Close]

BLAST of Bhi11G000428 vs. ExPASy Swiss-Prot
Match: P58045 (Cytochrome P450 71A14 OS=Arabidopsis thaliana OX=3702 GN=CYP71A14 PE=2 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 1.8e-17
Identity = 51/176 (28.98%), Postives = 102/176 (57.95%), Query Frame = 0


Query: 1   MRKICSFQLLTSLKIDSFSSIRRQELGLLIDRLREAAKNHSIVDLNSQVSSLIAGVICVM 60
           ++ +C   LL   K+ SF  +R +E+  +++R+ +A+ + S ++L+  + +L + V   +
Sbjct: 128 IKSVCIVNLLNKKKVQSFEKVREEEISEMMERVEKASSDSSPLNLSELLLTLTSDVTSRV 187

Query: 61  LFGKKFVDEE----FMAAIHEVTFLAGALNLGDFIPFIAFLD-LQGLGRRSKAISKAFDG 120
             G+K+  EE    F   + ++T L G   +G++IP +A++D L+G+  +++ +SKAF  
Sbjct: 188 SLGRKYSKEESMSDFKIQMRKITELVGGFPVGEYIPCLAWIDKLRGVDEKAEEVSKAFGD 247

Query: 121 FLNTIIEQHVEFKDQNNTKSGLFVDVMLDLMRSEKTKCQIDRTSIKGV---MFVIG 169
            +  ++++H++  D+       FVDV+L L R E+   QI R+ IK +   MF+ G
Sbjct: 248 LMEKVLQEHLDATDKPTLD---FVDVLLSLERHERNGVQIRRSDIKFLILDMFLAG 300

[Close]

BLAST of Bhi11G000428 vs. ExPASy Swiss-Prot
Match: P24465 (Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2)

HSP 1 Score: 87.8 bits (216), Expect = 1.5e-16
Identity = 53/173 (30.64%), Postives = 95/173 (54.91%), Query Frame = 0


Query: 1   MRKICSFQLLTSLKIDSFSSIRRQELGLLIDRLREAAKNHSIVDLNSQVSSLIAGVICVM 60
           +RKIC  +LL+  +++S+ SIR +E+GL+++R+ ++      V+L+  +  L +G I  +
Sbjct: 126 VRKICVLELLSIKRVNSYRSIREEEVGLMMERISQSCSTGEAVNLSELLLLLSSGTITRV 185

Query: 61  LFGKKFVDEE-----FMAAIHEVTFLAGALNLGDFIPFIAFLD-LQGLGRRSKAISKAFD 120
            FGKK+  EE     F     E+T L GA  +GD+ P  A++D L G+  R K      D
Sbjct: 186 AFGKKYEGEEERKNKFADLATELTTLMGAFFVGDYFPSFAWVDVLTGMDARLKRNHGELD 245

Query: 121 GFLNTIIEQHVEFKDQNNT---KSGLFVDVMLDLMRSEKTKCQIDRTSIKGVM 165
            F++ +I+ H+  +  N +   +    VDV+L L +       ++R ++K V+
Sbjct: 246 AFVDHVIDDHLLSRKANGSDGVEQKDLVDVLLHLQKDSSLGVHLNRNNLKAVI 298

[Close]

BLAST of Bhi11G000428 vs. NCBI nr
Match: XP_038904224.1 (cytochrome P450 71AU50-like [Benincasa hispida])

HSP 1 Score: 320.5 bits (820), Expect = 1.1e-83
Identity = 165/165 (100.00%), Postives = 165/165 (100.00%), Query Frame = 0


Query: 1   MRKICSFQLLTSLKIDSFSSIRRQELGLLIDRLREAAKNHSIVDLNSQVSSLIAGVICVM 60
           MRKICSFQLLTSLKIDSFSSIRRQELGLLIDRLREAAKNHSIVDLNSQVSSLIAGVICVM
Sbjct: 125 MRKICSFQLLTSLKIDSFSSIRRQELGLLIDRLREAAKNHSIVDLNSQVSSLIAGVICVM 184

Query: 61  LFGKKFVDEEFMAAIHEVTFLAGALNLGDFIPFIAFLDLQGLGRRSKAISKAFDGFLNTI 120
           LFGKKFVDEEFMAAIHEVTFLAGALNLGDFIPFIAFLDLQGLGRRSKAISKAFDGFLNTI
Sbjct: 185 LFGKKFVDEEFMAAIHEVTFLAGALNLGDFIPFIAFLDLQGLGRRSKAISKAFDGFLNTI 244

Query: 121 IEQHVEFKDQNNTKSGLFVDVMLDLMRSEKTKCQIDRTSIKGVMF 166
           IEQHVEFKDQNNTKSGLFVDVMLDLMRSEKTKCQIDRTSIKGVMF
Sbjct: 245 IEQHVEFKDQNNTKSGLFVDVMLDLMRSEKTKCQIDRTSIKGVMF 289

[Close]

BLAST of Bhi11G000428 vs. NCBI nr
Match: XP_031738787.1 (cytochrome P450 CYP736A12-like [Cucumis sativus])

HSP 1 Score: 230.7 bits (587), Expect = 1.1e-56
Identity = 120/165 (72.73%), Postives = 139/165 (84.24%), Query Frame = 0


Query: 1   MRKICSFQLLTSLKIDSFSSIRRQELGLLIDRLREAAKNHSIVDLNSQVSSLIAGVICVM 60
           MRKICSFQLLTS KI+SF+SIR QELG  IDRL+EAAKNH IVDL+ QVSSLIA VIC+M
Sbjct: 59  MRKICSFQLLTSSKINSFTSIRMQELGFFIDRLQEAAKNHVIVDLSYQVSSLIADVICIM 118

Query: 61  LFGKKFVDEEFMAAIHEVTFLAGALNLGDFIPFIAFLDLQGLGRRSKAISKAFDGFLNTI 120
           LFGKKF D+EFM AIHE TFLAGA NLGDFIPFIAFLDLQGLGRR+KA+ K FD  L+ I
Sbjct: 119 LFGKKFTDKEFMVAIHEGTFLAGASNLGDFIPFIAFLDLQGLGRRAKAVIKIFDELLDVI 178

Query: 121 IEQHVEFKDQNNTKSG-LFVDVMLDLMRSEKTKCQIDRTSIKGVM 165
           IE+ +E+K QN T+ G  FVDVMLD++RS + + QIDR+SIK V+
Sbjct: 179 IEERLEYKKQNRTEKGEFFVDVMLDVIRSREKEHQIDRSSIKAVI 223

[Close]

BLAST of Bhi11G000428 vs. NCBI nr
Match: XP_008449122.2 (PREDICTED: cytochrome P450 CYP736A12-like [Cucumis melo])

HSP 1 Score: 228.0 bits (580), Expect = 7.3e-56
Identity = 119/166 (71.69%), Postives = 139/166 (83.73%), Query Frame = 0


Query: 1   MRKICSFQLLTSLKIDSFSSIRRQELGLLIDRLREAAKNHSIVDLNSQVSSLIAGVICVM 60
           MRKICS  LLTS K++SFSSIRRQELGLL+DRL+EAA+NH+IVDL+SQVSSL   VICVM
Sbjct: 125 MRKICSSHLLTSSKVNSFSSIRRQELGLLVDRLQEAARNHAIVDLSSQVSSLTFDVICVM 184

Query: 61  LFGKKFVDEEFMAAIHEVTFLAGALNLGDFIPFIAFLDLQGLGRRSKAISKAFDGFLNTI 120
           LFGKKFVD+E   AI E TFL+G  NLGDFIPFIAFLDLQGL RR+KAI+K  DGFL+ I
Sbjct: 185 LFGKKFVDKELTVAIREGTFLSGVPNLGDFIPFIAFLDLQGLCRRAKAINKVVDGFLDMI 244

Query: 121 IEQHVEFKDQNNT-KSGLFVDVMLDLMRSEKTKCQIDRTSIKGVMF 166
           IE+  EFKDQN T K G FVDVMLDL RS++ + QIDR+++K V+F
Sbjct: 245 IEERPEFKDQNKTDKGGFFVDVMLDLTRSQEIEHQIDRSNVKAVLF 290

[Close]

BLAST of Bhi11G000428 vs. NCBI nr
Match: XP_011652733.2 (cytochrome P450 CYP736A12 [Cucumis sativus])

HSP 1 Score: 224.6 bits (571), Expect = 8.1e-55
Identity = 117/166 (70.48%), Postives = 141/166 (84.94%), Query Frame = 0


Query: 1   MRKICSFQLLTSLKIDSFSSIRRQELGLLIDRLREAAKNHSIVDLNSQVSSLIAGVICVM 60
           MRKICS  LLTS K++SFSSIRRQELGLLI  L+EAA+NH+IVDL+S++SSL   VICVM
Sbjct: 59  MRKICSSHLLTSSKVNSFSSIRRQELGLLIHHLKEAARNHAIVDLSSKISSLTFDVICVM 118

Query: 61  LFGKKFVDEEFMAAIHEVTFLAGALNLGDFIPFIAFLDLQGLGRRSKAISKAFDGFLNTI 120
           LFGKKFVD+E  AAI E T L+GA NLGDF PFIAFLDLQGLGRR+KA++K  DGFL+ I
Sbjct: 119 LFGKKFVDKELTAAIREGTSLSGAPNLGDFFPFIAFLDLQGLGRRAKAVNKVVDGFLDMI 178

Query: 121 IEQHVEFKDQNNTKSG-LFVDVMLDLMRSEKTKCQIDRTSIKGVMF 166
           IE+ +EFKD+N T+S  LFVDVMLDL+RSE+ + QIDR++IK V+F
Sbjct: 179 IEERLEFKDKNKTESSELFVDVMLDLIRSEEMEHQIDRSNIKAVIF 224

[Close]

BLAST of Bhi11G000428 vs. NCBI nr
Match: XP_023007802.1 (cytochrome P450 CYP736A12-like [Cucurbita maxima])

HSP 1 Score: 200.7 bits (509), Expect = 1.2e-47
Identity = 102/164 (62.20%), Postives = 131/164 (79.88%), Query Frame = 0


Query: 1   MRKICSFQLLTSLKIDSFSSIRRQELGLLIDRLREAAKNHSIVDLNSQVSSLIAGVICVM 60
           MRKIC  QL T+LK++SF+S+RR+E+GLLID L+EAAK H +VDL+SQ+SS IA VIC M
Sbjct: 125 MRKICILQLFTTLKMNSFTSMRREEVGLLIDHLQEAAKMHGVVDLSSQISSFIADVICRM 184

Query: 61  LFGKKFVDEEFMAAIHEVTFLAGALNLGDFIPFIAFLDLQGLGRRSKAISKAFDGFLNTI 120
           +FG+KFV+EEF A+IHE T L GA +LGDFIPFI+ LDLQGLGRR+K ++K FDGFL+ I
Sbjct: 185 VFGRKFVEEEFKASIHEATLLVGAPDLGDFIPFISMLDLQGLGRRAKGVTKVFDGFLDMI 244

Query: 121 IEQHVEFKDQNNTKSGLFVDVMLDLMRSEKTKCQIDRTSIKGVM 165
           I+QH+E  D+N TK G F+DV+LD         +ID +SIKGV+
Sbjct: 245 IKQHLESNDENRTK-GCFLDVLLD--------SKIDHSSIKGVI 279

[Close]

BLAST of Bhi11G000428 vs. ExPASy TrEMBL
Match: A0A1S3BLZ4 (cytochrome P450 CYP736A12-like OS=Cucumis melo OX=3656 GN=LOC103491077 PE=3 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 3.5e-56
Identity = 119/166 (71.69%), Postives = 139/166 (83.73%), Query Frame = 0


Query: 1   MRKICSFQLLTSLKIDSFSSIRRQELGLLIDRLREAAKNHSIVDLNSQVSSLIAGVICVM 60
           MRKICS  LLTS K++SFSSIRRQELGLL+DRL+EAA+NH+IVDL+SQVSSL   VICVM
Sbjct: 125 MRKICSSHLLTSSKVNSFSSIRRQELGLLVDRLQEAARNHAIVDLSSQVSSLTFDVICVM 184

Query: 61  LFGKKFVDEEFMAAIHEVTFLAGALNLGDFIPFIAFLDLQGLGRRSKAISKAFDGFLNTI 120
           LFGKKFVD+E   AI E TFL+G  NLGDFIPFIAFLDLQGL RR+KAI+K  DGFL+ I
Sbjct: 185 LFGKKFVDKELTVAIREGTFLSGVPNLGDFIPFIAFLDLQGLCRRAKAINKVVDGFLDMI 244

Query: 121 IEQHVEFKDQNNT-KSGLFVDVMLDLMRSEKTKCQIDRTSIKGVMF 166
           IE+  EFKDQN T K G FVDVMLDL RS++ + QIDR+++K V+F
Sbjct: 245 IEERPEFKDQNKTDKGGFFVDVMLDLTRSQEIEHQIDRSNVKAVLF 290

[Close]

BLAST of Bhi11G000428 vs. ExPASy TrEMBL
Match: A0A0A0L2B1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G067750 PE=3 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 2.3e-55
Identity = 117/168 (69.64%), Postives = 142/168 (84.52%), Query Frame = 0


Query: 1   MRKICSFQLLTSLKIDSFSSIRRQELGLLIDRLREAAKNHSIVDLNSQVSSLIAGVICVM 60
           MRKICS  LLTS K++SFSSIRRQELGLLI  L+EAA+NH+IVDL+S++SSL   VICVM
Sbjct: 125 MRKICSSHLLTSSKVNSFSSIRRQELGLLIHHLKEAARNHAIVDLSSKISSLTFDVICVM 184

Query: 61  LFGKKFVDEEFMAAIHEVTFLAGALNLGDFIPFIAFLDLQGLGRRSKAISKAFDGFLNTI 120
           LFGKKFVD+E  AAI E T L+GA NLGDF PFIAFLDLQGLGRR+KA++K  DGFL+ I
Sbjct: 185 LFGKKFVDKELTAAIREGTSLSGAPNLGDFFPFIAFLDLQGLGRRAKAVNKVVDGFLDMI 244

Query: 121 IEQHVEFKDQNNTKSG-LFVDVMLDLMRSEKTKCQIDRTSIKGVMFVI 168
           IE+ +EFKD+N T+S  LFVDVMLDL+RSE+ + QIDR++IK V+F +
Sbjct: 245 IEERLEFKDKNKTESSELFVDVMLDLIRSEEMEHQIDRSNIKAVIFAL 292

[Close]

BLAST of Bhi11G000428 vs. ExPASy TrEMBL
Match: A0A6J1KZP5 (cytochrome P450 CYP736A12-like OS=Cucurbita maxima OX=3661 GN=LOC111500301 PE=3 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 6.0e-48
Identity = 102/164 (62.20%), Postives = 131/164 (79.88%), Query Frame = 0


Query: 1   MRKICSFQLLTSLKIDSFSSIRRQELGLLIDRLREAAKNHSIVDLNSQVSSLIAGVICVM 60
           MRKIC  QL T+LK++SF+S+RR+E+GLLID L+EAAK H +VDL+SQ+SS IA VIC M
Sbjct: 125 MRKICILQLFTTLKMNSFTSMRREEVGLLIDHLQEAAKMHGVVDLSSQISSFIADVICRM 184

Query: 61  LFGKKFVDEEFMAAIHEVTFLAGALNLGDFIPFIAFLDLQGLGRRSKAISKAFDGFLNTI 120
           +FG+KFV+EEF A+IHE T L GA +LGDFIPFI+ LDLQGLGRR+K ++K FDGFL+ I
Sbjct: 185 VFGRKFVEEEFKASIHEATLLVGAPDLGDFIPFISMLDLQGLGRRAKGVTKVFDGFLDMI 244

Query: 121 IEQHVEFKDQNNTKSGLFVDVMLDLMRSEKTKCQIDRTSIKGVM 165
           I+QH+E  D+N TK G F+DV+LD         +ID +SIKGV+
Sbjct: 245 IKQHLESNDENRTK-GCFLDVLLD--------SKIDHSSIKGVI 279

[Close]

BLAST of Bhi11G000428 vs. ExPASy TrEMBL
Match: A0A6J1F1R2 (cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111438620 PE=3 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 1.3e-47
Identity = 102/164 (62.20%), Postives = 131/164 (79.88%), Query Frame = 0


Query: 1   MRKICSFQLLTSLKIDSFSSIRRQELGLLIDRLREAAKNHSIVDLNSQVSSLIAGVICVM 60
           MRKIC  QL T+LK++SF+S+RR+E+GLLID L+EAAK H +VDL+S++SSLIA VIC M
Sbjct: 125 MRKICILQLFTTLKMNSFTSMRREEVGLLIDHLQEAAKMHGVVDLSSKISSLIADVICRM 184

Query: 61  LFGKKFVDEEFMAAIHEVTFLAGALNLGDFIPFIAFLDLQGLGRRSKAISKAFDGFLNTI 120
           +FG+KFV+EEF A+IHE T L GA NLGDFIPFI+ LDL GLGRR+K ++K FDGFL+ I
Sbjct: 185 VFGRKFVEEEFKASIHEATLLVGAPNLGDFIPFISMLDLHGLGRRAKGVTKVFDGFLDII 244

Query: 121 IEQHVEFKDQNNTKSGLFVDVMLDLMRSEKTKCQIDRTSIKGVM 165
           I+QH+E  D+N TKS  F+DV+LD         +ID +SIKGV+
Sbjct: 245 IKQHLESNDENRTKS-CFLDVLLD--------SKIDHSSIKGVI 279

[Close]

BLAST of Bhi11G000428 vs. ExPASy TrEMBL
Match: A0A5D3DKJ7 (Cytochrome P450 CYP736A12-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold943G00260 PE=3 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 2.5e-46
Identity = 100/140 (71.43%), Postives = 116/140 (82.86%), Query Frame = 0


Query: 1   MRKICSFQLLTSLKIDSFSSIRRQELGLLIDRLREAAKNHSIVDLNSQVSSLIAGVICVM 60
           MRKICS  LLTS K++SFSSIRRQELGLL+DRL+EAA+NH+IVDL+SQVSSL   VICVM
Sbjct: 20  MRKICSSHLLTSSKVNSFSSIRRQELGLLVDRLQEAARNHAIVDLSSQVSSLTFDVICVM 79

Query: 61  LFGKKFVDEEFMAAIHEVTFLAGALNLGDFIPFIAFLDLQGLGRRSKAISKAFDGFLNTI 120
           LFGKKFVD+E   AI E TFL+G  NLGDFIPFIAFLDLQGL RR+KAI+K  DGFL+ I
Sbjct: 80  LFGKKFVDKELTVAIREGTFLSGVPNLGDFIPFIAFLDLQGLCRRAKAINKVVDGFLDMI 139

Query: 121 IEQHVEFKDQNNTKSGLFVD 141
           IE+  EFKDQN T   + ++
Sbjct: 140 IEERPEFKDQNKTDKDIMIN 159

The following BLAST results are available for this feature:

BLAST of Bhi11G000428 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G24960.1	1.3e-18	28.98	cytochrome P450, family 71, subfamily A, polypeptide 14	[more]
AT5G06900.1	2.4e-17	31.61	cytochrome P450, family 93, subfamily D, polypeptide 1	[more]
AT2G30770.1	3.5e-16	25.28	cytochrome P450, family 71, subfamily A, polypeptide 13	[more]
AT4G20240.1	1.1e-14	26.06	cytochrome P450, family 71, subfamily A, polypeptide 27	[more]
AT5G07990.1	2.1e-13	29.79	Cytochrome P450 superfamily protein	[more]

BLAST of Bhi11G000428 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A068Q5V6	2.2e-31	44.97	Cytochrome P450 71AU50 OS=Prunus mume OX=102107 GN=CYP71AU50 PE=2 SV=1	[more]
H2DH18	2.5e-22	34.52	Cytochrome P450 CYP736A12 OS=Panax ginseng OX=4054 PE=2 SV=1	[more]
Q50EK4	3.6e-21	31.84	Cytochrome P450 750A1 OS=Pinus taeda OX=3352 GN=CYP750A1 PE=2 SV=1	[more]
P58045	1.8e-17	28.98	Cytochrome P450 71A14 OS=Arabidopsis thaliana OX=3702 GN=CYP71A14 PE=2 SV=1	[more]
P24465	1.5e-16	30.64	Cytochrome P450 71A1 OS=Persea americana OX=3435 GN=CYP71A1 PE=1 SV=2	[more]

BLAST of Bhi11G000428 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038904224.1	1.1e-83	100.00	cytochrome P450 71AU50-like [Benincasa hispida]	[more]
XP_031738787.1	1.1e-56	72.73	cytochrome P450 CYP736A12-like [Cucumis sativus]	[more]
XP_008449122.2	7.3e-56	71.69	PREDICTED: cytochrome P450 CYP736A12-like [Cucumis melo]	[more]
XP_011652733.2	8.1e-55	70.48	cytochrome P450 CYP736A12 [Cucumis sativus]	[more]
XP_023007802.1	1.2e-47	62.20	cytochrome P450 CYP736A12-like [Cucurbita maxima]	[more]

BLAST of Bhi11G000428 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3BLZ4	3.5e-56	71.69	cytochrome P450 CYP736A12-like OS=Cucumis melo OX=3656 GN=LOC103491077 PE=3 SV=1	[more]
A0A0A0L2B1	2.3e-55	69.64	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G067750 PE=3 SV=1	[more]
A0A6J1KZP5	6.0e-48	62.20	cytochrome P450 CYP736A12-like OS=Cucurbita maxima OX=3661 GN=LOC111500301 PE=3 ...	[more]
A0A6J1F1R2	1.3e-47	62.20	cytochrome P450 CYP736A12-like OS=Cucurbita moschata OX=3662 GN=LOC111438620 PE=...	[more]
A0A5D3DKJ7	2.5e-46	71.43	Cytochrome P450 CYP736A12-like protein OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..168 e-value: 5.4E-18 score: 66.8
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 1..165
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 2..161 e-value: 2.1E-10 score: 40.0
None	No IPR available	PANTHER	PTHR47943	CYTOCHROME P450 93A3-LIKE	coord: 1..191
None	No IPR available	PANTHER	PTHR47943:SF2	CYTOCHROME P450 FAMILY 71 PROTEIN	coord: 1..191

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi11M000428	Bhi11M000428	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen