Bhi11G000412 (gene) Wax gourd (B227) v1

Overview
NameBhi11G000412
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionDNA topoisomerase I
Locationchr11: 12454539 .. 12462293 (+)
RNA-Seq ExpressionBhi11G000412
SyntenyBhi11G000412
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAATTGCATAAAAAGAACCACAAATCTGTTGGGTGGAAGACCCTCTTTGCTTTCTTTTTTGGTTTTTATTTTTCTGTGCAAACAAATAAACCATCTCCTTCTCCTTTTCCGATTCACGACAAACATTTTTTTAGGTTTCCTTCGACCTCTCCCTCGCACTCGCTCTCTCTCCCTCTCTCTCTTCAATTGCTTCCATTAACAAGCGACTGAGGTTGCTCCGAACCTCACACCGGCCACCCTCTTCACCTCAGGTACTCTCTCTAACTCTTGTTCTTAAGCTTCAGATTTCCCCCAATCTCACTCTGCTTCAGCTCAATGAACCGTGTAGTTCGATTCCCTTTTGCTTCTCTTCTTGCATTCGAAACCTACCCTTGTAATTTCGGCCCTTTCTGAAGTGATTTAGTTGCTTTTTTATTCAATTTTTGTAGTCTGTAATTCTCTGCTTGCTTGGATTAGCTTATCTAATGTTCTAGGTTTAACTTTGCTTTCTTAGAGGCTCTTTTCAATGATTTAGTTTAAGTAATGAACACCCTTTGGTTAGATTTCTGAGACCGAAGTATTTACCCTTTTGCCCTGTACTTTTTATAAATTCTCCAATGAATGGGGTATTTTCCAACGAACGTTTTGAGTGTAATTGTTAGGTTAAGTGTTTACGCAAAGGGTATTATTGGTGCAGAAACTGAACTTGTCTGTCTTCTTCATCTTCCAAGAGACTGATGTAAAATAATTTCTGGTTCTCTGTACATAGCTGCGCAATGGCTGTTGAGACTTTTGGAAAAGCTCCAATGTTTGATGACGATGACGATGAGGACAACATGCCGATAGTTTTTAAAAGGAGTAGTGCATCTAGGCAAAATCAGTTAGATTCGGAAATTAAGCATTCACAATCTCAGAGATTGAATAAGTTATCGGGGAGGCAGGGTTCTGATATATGTTCAACCAATGGCCAAAGCTCTAATAATCAAAAAAGGATGACTTCATTGCCGAAGACATCACCCATGAGATCCGAAATAGGAAGTCAGAAAGCATCCAAGTCTCCTATCCGGGAACCACCTGTTAAACCACTAGGCACATCTTTAAAAGTTTCAGATTCTATGGATAGCCGGTCGAAACCTTTGGTAAACACTGCTGTGAAAAAGGAGAATGTTTCTATAATTCTTACTCAAGAGAATAGTGACTCTGAAGACGATAAACCATTGAGTATTAGATTAAAAGGGAACTCCAGCCAGCCTAATAAAGGTCTTGTACCAACCGTACCAAAGAACAAAGTAAAAAAATCATCAGGAGATTCTGATGATGAAATTCCTTTGTCATCAAAATTTCAAATGAAATCTGGTTCAGGGGTGTTGGGTAGTAAACAACATGGTTTCGATGAGAAGAAACCATTGGTTCCAAGAGTACATCAGAATGGTTCGACCTCAAGGGATAAGCTGCAAAAGCCTGCTGTTCTTTCCAGCAAGAGACCTCTTCCTAATGCAGATTCCCATACCCCTTCCCAAATTTCAACAAAAAAGCCAAAGCTTTCAGATTCATCTACTCCTTTGAGTAGCAAGCTAGTATCATTGAAAGCAAAACAAGAAGTAGATGACGAGGACGACGATATTCCTATATCACAGAGAATGAAGAAGATGTCTGCACCAGTGAATAAGTCATCAAGCTCTAAGCAAACAGTATCAAAAGTTGTTTCTTCATCAATCAAGAAAACATTCAAAAAGTCCAAAAAGCCAGTCAAGAAATCTAAATATGTTAAGTCCACAAAGTTGCTGCCTAGTGCCGGTGATGGACAGAAAAAATGGACCACCTTGGTTCACAATGGTGTCATTTTCCCACCTCCATACCAGACTCACGGGGTGAAGATACTTTATAAAGGGAAGCCTGTTGATTTGACTCCGGAACAAGAGGAGGTACCCCTCCACTTGACGTGTTGCATGCACAACATGTTTCTTCCATTTTCTTTATTATTATTATTATTATTATTATCATCATAATATATTTGTATGTAATTTATGTCATACACAGTTCATCTTTACCATCGCATTGTGGTAAATATGGTTGATATGAAAATTTGTTGTTATCAGGTTGCAACTATGTTTGCTGTGATGAAGGATACAGAATATATGCTAAAACCAAAATTCAAAGAAAATTTCTGGAATGACTGGCGCAAATTACTTGGGAAAAACCATGTCATTCAAAATTTGGATGATTGTGATTTCACCCCAATATATGACTGGTATCAGAAGGAAAAGGAGAAGAAGAAACAGATGACTACAGAAGTAAGTCCATGATGTTTCAATCTTCCTAGTTGTTTATTATATCTTTTCATCTTTACAAGTTTAATGGAAACATAGTTCCAAATTTTGGCATCTTTCTTGGGTCTCGATCTACTTTTTCATTATCAAATCCTAAGAAGTAATAAGGATGTTTTCAGTGAAAAGGAATGTCTGGTTTCATTTCTAAATGTTACAAACTTCTTTTTTGATGGTTTATTCTTAGGTTTCCCAAAAATCCATAATTTGTTTTTTTGTTCATATATTTTTCTAGAGGTTTTACTTTAAGTTCAATTTTTAGGTTTTCTTTTCATGCCTTGGTTATATGGGTATGTTGCCAAGGTGTATTTCAAGGTGCTTTTGCTCCATTTCTCAATGTGAGCTATTCGTTTTTGATACAAGGAAATATGAACTATTTATCATTCAAGATGCTTTATTTTTGGCGGCCACTAAATGTCTAACCATAATAGTTACTTCTACTCGATACTCGATTTCAAGTTAATTTGTTTTGTTTTTTTTTTTTTTTTTCTAATTAAGGGTGAATTTGATGTATATTGTTGAACTAGTCATCTTTAATTGATTAATCTGCAACCTTGTATGAAGCCAACTATGAGCTTCAGGCCCTTTTCAGGCATTCAATTTCATTAATTCTGGTTTCACATCTCTGGAATTTGCTTGTCCAGGAGAAGAAAGCTCTAAAGGAGGAGAAACTGAAACAAGAGGAGAAGTACATGTGGGCTATTCTTGATGGTGTTAAGGAGAAGGTTATTCTCATTTACCTTTTTCTACTTGCTCTTTGGAAGAATATAGTCTTGAGCATATTCTTTATTTACTTTGATTACCTTCCCTTGCAATGATTGTCCAGTGTAGTCAATGTTAGAGCACTCACTGAATGAATGTTGCTCAACTAGGGATACTGTAATAGACTTGTTGCATTCTACGGTAATCACTAATCAGCAGTTGGCCACAACTTAAGGTCATACAGTCTTTTCTTTTTTGGTTAATTTCACATGCCATTCATGGTCATAGGAGTATTGAAATCAACCATCGAACCATGCAATTGAACACACTAGGGGATTATTTATTTATTTGTTCGTTTTAAATTTCAAGTAGTTTGTTTTGAAGTGAGAGAAATCGTTTTATGTTTGCTGTATTCAAAACCAAACGAGAAAATTGGGCAGTTCTAGTGTCAAGAAAGTTGTCATAGTTTTGAAAATTCAAAAGCTTGGTGATATTCTACTTGGATGCAAGCAATAAGAGTATTTTCACAGTAAGTACCAGCTTAGAGGGGTGTTGACTGGTGGTAATGCTTGATTAATTACACTGCAGATCGTCATATGATAGATACTTGTTTACACTTGTCTGCTTCATACTAAATTACTAAGTTCATGCGCTAGTTGCTATTGCTACTATGTACTTTATAAGTTCATCTTAGCAATCGGTTTCTGCAATCTTATCCATTGAATCATTCCGGTAGAGCATTTACACAGTTTTTTGTTCTTAATATGGATCTCAGGTTGGAAACTTTAGAGTTGAACCGCCTGGACTGTTCCGAGGCCGTGGAGAACATCCAAAGGTACCATATTTAGGATTTAGTAAGTTACATATTTACTAGCATTTATGACACAAATCGGGTAATTCTTCAAGGTACTCCTTGGAGAGATGAGTATATGTTTTGGTATTTGGATGTCCATAGTTTACCCTTATTTGGAGATTTGTTACTTTATTCAGCTTTTAAAGTTTGGTTTTCTTTTCAGATGGGGAAATTGAAAAGGCGCATTCACCCCAGTGACATTACAATTAATATTGGAAAAGGTGCTCCAATCCCTGAGTGTCCTATACCTGGTGAAAGGTTTGATTCTTCTTTACACCACCATAAATAAGTTGCATTAGAAATATTGCAAAATAAGGGGCTATGTGCGTCTAAAAATTCATGAGATACATACCTAAACTGTTTCAGAATTTCTCAGTTTTATCATTTTCAATTTTTCATTAAATACTGGGTTCTTTGACAGGTGGAAGGAAGTGAGACATGACAATACGGTGACATGGTTGGCTTTCTGGAATGATCCAATAAATCAAAAAGAATTCAAGTATGTGTTTCTGGCTGCTAGTAGTTCACTGAAAGGCCAGAGTGACAAAGAGAAATACGAGAAAGCAAGGATGCTCAAGGTGTGCCATTCATTATAATTTTACAATTCTTTGTTGAAGTTGTAGTATGGAATCATCATGTCTGTTGAACCATTGCTGTTTTGTGTAGGACTACATAAAAAACATAAGGAATGCATATACAAAGGATTTTACAAGCAAAGACTCTACACGAAGGCAAATTGCAGTTGCTACTTATCTCATTGATAAACTGGCTCTTAGGGCTGGGAATGAGAAAGTAATGTCATCTTAGCCTATTTATGTTTCCCATGCATGCCATATTTTATATTTTCCACCATTTTGATCTGGAAGAGTTAAAATGTTGTTGATCCCTGCTGCAGGATGACGATGAAGCTGATACAGTTGGCTGCTGTACACTGAAAGTAGAGAATGTTAAAGTGGTACCTCCGAATCTTCTGGAGGTAAATATCTTTAAGCTTATTGAAATAGGAGCTTGAAAGTTGAGAGATGTCTTGTTTTTAATGCCCACAAGAGATGACGGCTTGATTCTTTATTTCAGTTCAACTTCCTCGGTAAAGATTCAATCAAGTATGAAAATACTGTTGAGGTTGAGCTCCCTGTTTTCAAAGCAATCACACAATTCCAAAGTGGTGAGCTTGTTTATCTGTTTGTTCGTTTATTTTTTTCTTTATCCTTTTTTTTATATAAATAATTTTATTTAAATACAACGATGCGTCAGTGTCTTGACTGAGGCTGTAGAGTATCATGTTTTTACCTGCTTCAGGAAAAAGTGGAACCGATGATCTGTTCGACAAGTTGGATACGAGTAAATTGAATGCGCATCTGAAGGATCTCATGCCTGGCCTCACTGCAAAAGTGTTCCGTACATATAATGCTTCAATCACACTTGATGACATGGTAAAATATATTTGGAGCATCAGTTTTTCATCTACCCTTAATTTGTTGCAGGGAACTGATTGTGAGTTCAAAATTTGTTGTAATCTACGAGAGTAAACAACGTTATCCTTTTCAAAATAACTCTAGCAGGATGTTTGATTCTTAGACATGGGTCTACAGAGTGCGGTTTCACAATCCCATTCCTTGTAATCCAAATATGAGATTACCCTAATCTTGTTCCCAGGCTTTCCATCCTAGCAAATCAAAATAATCTTATTCTCATGTACCAAATACTACTTTCACTTTATCCCTCTACCATCCAAACTCAGATAATTTGGCGTAGGGCTAAACGGAGATAATTTAGGCCCCATTTGGTAACCATTTTGTTTTTTGTTTTTGTTTTTGAAAATTAAGCCCTATAAACATTACTTCCACCTCCCAATTTCTTTCTTTATTATCTACTTTTCACTCTGGTTTAAAAAACCAAGTCAAATTTTGAGAACTAAAAAAAGTAGCTTTCAAAAGGATGTTTTTGTTTTTGAAATTTGGCTAAGAATTAAACCATTATACTCAAGAAAGATGCAAATCATGGTAAGAAATGTGGATAATATAAGCTTAATTTTCAATAACAAAAACAAAAAAACCAAATGATTACCAATCGGGTCATAGCTTTACTCTTTTGGATTGGGATCCCTTTCTGCAATTGGGTGTGTGGTTGTTTTTATTTATTTATTTATTTTTGTATACCTTTGTATATCCTTTCATCATCCTCAATGTTTTAAAAAAAAGAACTCTGATATCCTTGTTTTATAACTCGTAAGTCTCAACTCTCAAGCTTCCTATTCCGATGACTGCAACCAAATGCCCCTGAAGTTCTATGGACTATTGCAATTGGGATCATTTGGTTTTCCTTAAATCTTTTCCATCACAAACTTTTAAGCATTCATCTACTTCATCATAGGTTAGGTTCTGACAGTGGAAAGTCATTTGTGTTTTATATCTTGCTCTCACGAACCATTTATTTGATACTTCTACAGCATAATGATGATGTCAAAGTTGTCCATGAGGCATCAAATTTTAGATGTTTGCTCTCCTAGGTGGTAAAATCATTGTCCTGATCGAAGTTTTTATAAACACACTCCATTGGATGAAGAAGGGCATTTTAATGGTTGACTTGCATGGAATTTTAACGGATGGGGACTTGGGTCTAGTTGATCTAGGTTAAGTTCATAGAAATCATTCTGCTTATTATTCGAGTCAATCTGTTTACTTTTGTGATTTTCCTTTTTTCTCCTTTTATTTAATTTAGATTTTTAATAAGATTATGACGTTATCCATGACAGTTGAATAGAGGAACCAAAGATGGAGACGTTGTAGAAAAGATTGCCACATACCAGCATGCAAACAAGGAGGTAAATTCGCTCCTCTTTAGGGTTTTACTAGTCTAATTTCATGCCATTTGACATTGGCCAATTTTTCTATGAACTACAGGTTGCAATTATTTGTAATCACCAGCGGACTGTTTCAAAATCACATGGGGCGCAAATGACGAGATTGAATGAAAAGATCTCTGAGCTCAGGGTAATAACCATATTTTTCTCATGGTCCATCTTGGTTAAATAATGCTGCCAGTTCACTTTGTAGCTCTAGTGCATTTAGTCTATCCATCATTTGTTATGCAGGACATTGTGAAAGAGCTTAAAATTGATTTAGATAGAGCAAAGAAAGGGAAGCCCCCTTTAAAAGATGCTGATGGAAAGCGAAAGAGGAACTTGACCCCGGAAGCGTAAACTTCCTCATTTACTTGAAAAAGCCACATTAACATCTTCATTTGTAGTGTTTAACTTTAAGAACTCATATTTCAGGTTAGAGAAGAAGATATCCCAAACGAATGCAAAGATTGAGAAGATAGAGCGAGATATGAAGACTAAAGAGGATTTGAAAACTGTAGCATTGGGCACGTCAAAGATCAACTACCTTGATCCTCGGATCACAGTCGCATGGTGTAAGCGTCATGAAGTCCCTATCGAAAAGGTAAGTACCATTTTGTTGCATTCATGATGCACCTAGCAAGACATTTTGAATCAAACTGTTTATATCTCCTTTTTTTTATGGATTTTAAAGATCACTTTATAACATACTCCTACAGATATTCAACAAGTCTCTTCTAGCAAAGTTTGCTTGGGCAATGGACGTCGATCCCGACTTCAGATTCTGATTGTTGTTTATATTCCTGGCGGTCTCAAACCCTAAATCATCATCCTTCGCCCCCGGCTCAACCTCGAAAGAAAATAAGAAAAAATGAAGAAGAGATGGAGTAGGAAAAATTGAACTGTACTATTTTGCATTACATTCAATTTTTTCTTTTTAATCCTTTAGTTTGCAGAGGAAAGTCCCATGATGTATGTTTATCTATCTAATTATATTTGGGGTAAAATGGCTCATTTCTGACACTCCCCATTCCCCATTCCCCTTCCCC

mRNA sequence

ATGAAATTGCATAAAAAGAACCACAAATCTGTTGGGTGGAAGACCCTCTTTGCTTTCTTTTTTGGTTTTTATTTTTCTGTGCAAACAAATAAACCATCTCCTTCTCCTTTTCCGATTCACGACAAACATTTTTTTAGGTTTCCTTCGACCTCTCCCTCGCACTCGCTCTCTCTCCCTCTCTCTCTTCAATTGCTTCCATTAACAAGCGACTGAGGTTGCTCCGAACCTCACACCGGCCACCCTCTTCACCTCAGCTGCGCAATGGCTGTTGAGACTTTTGGAAAAGCTCCAATGTTTGATGACGATGACGATGAGGACAACATGCCGATAGTTTTTAAAAGGAGTAGTGCATCTAGGCAAAATCAGTTAGATTCGGAAATTAAGCATTCACAATCTCAGAGATTGAATAAGTTATCGGGGAGGCAGGGTTCTGATATATGTTCAACCAATGGCCAAAGCTCTAATAATCAAAAAAGGATGACTTCATTGCCGAAGACATCACCCATGAGATCCGAAATAGGAAGTCAGAAAGCATCCAAGTCTCCTATCCGGGAACCACCTGTTAAACCACTAGGCACATCTTTAAAAGTTTCAGATTCTATGGATAGCCGGTCGAAACCTTTGGTAAACACTGCTGTGAAAAAGGAGAATGTTTCTATAATTCTTACTCAAGAGAATAGTGACTCTGAAGACGATAAACCATTGAGTATTAGATTAAAAGGGAACTCCAGCCAGCCTAATAAAGGTCTTGTACCAACCGTACCAAAGAACAAAGTAAAAAAATCATCAGGAGATTCTGATGATGAAATTCCTTTGTCATCAAAATTTCAAATGAAATCTGGTTCAGGGGTGTTGGGTAGTAAACAACATGGTTTCGATGAGAAGAAACCATTGGTTCCAAGAGTACATCAGAATGGTTCGACCTCAAGGGATAAGCTGCAAAAGCCTGCTGTTCTTTCCAGCAAGAGACCTCTTCCTAATGCAGATTCCCATACCCCTTCCCAAATTTCAACAAAAAAGCCAAAGCTTTCAGATTCATCTACTCCTTTGAGTAGCAAGCTAGTATCATTGAAAGCAAAACAAGAAGTAGATGACGAGGACGACGATATTCCTATATCACAGAGAATGAAGAAGATGTCTGCACCAGTGAATAAGTCATCAAGCTCTAAGCAAACAGTATCAAAAGTTGTTTCTTCATCAATCAAGAAAACATTCAAAAAGTCCAAAAAGCCAGTCAAGAAATCTAAATATGTTAAGTCCACAAAGTTGCTGCCTAGTGCCGGTGATGGACAGAAAAAATGGACCACCTTGGTTCACAATGGTGTCATTTTCCCACCTCCATACCAGACTCACGGGGTGAAGATACTTTATAAAGGGAAGCCTGTTGATTTGACTCCGGAACAAGAGGAGGTTGCAACTATGTTTGCTGTGATGAAGGATACAGAATATATGCTAAAACCAAAATTCAAAGAAAATTTCTGGAATGACTGGCGCAAATTACTTGGGAAAAACCATGTCATTCAAAATTTGGATGATTGTGATTTCACCCCAATATATGACTGGTATCAGAAGGAAAAGGAGAAGAAGAAACAGATGACTACAGAAGAGAAGAAAGCTCTAAAGGAGGAGAAACTGAAACAAGAGGAGAAGTACATGTGGGCTATTCTTGATGGTGTTAAGGAGAAGGTTGGAAACTTTAGAGTTGAACCGCCTGGACTGTTCCGAGGCCGTGGAGAACATCCAAAGATGGGGAAATTGAAAAGGCGCATTCACCCCAGTGACATTACAATTAATATTGGAAAAGGTGCTCCAATCCCTGAGTGTCCTATACCTGGTGAAAGGTGGAAGGAAGTGAGACATGACAATACGGTGACATGGTTGGCTTTCTGGAATGATCCAATAAATCAAAAAGAATTCAAGTATGTGTTTCTGGCTGCTAGTAGTTCACTGAAAGGCCAGAGTGACAAAGAGAAATACGAGAAAGCAAGGATGCTCAAGGACTACATAAAAAACATAAGGAATGCATATACAAAGGATTTTACAAGCAAAGACTCTACACGAAGGCAAATTGCAGTTGCTACTTATCTCATTGATAAACTGGCTCTTAGGGCTGGGAATGAGAAAGATGACGATGAAGCTGATACAGTTGGCTGCTGTACACTGAAAGTAGAGAATGTTAAAGTGGTACCTCCGAATCTTCTGGAGTTCAACTTCCTCGGTAAAGATTCAATCAAGTATGAAAATACTGTTGAGGTTGAGCTCCCTGTTTTCAAAGCAATCACACAATTCCAAAGTGGAAAAAGTGGAACCGATGATCTGTTCGACAAGTTGGATACGAGTAAATTGAATGCGCATCTGAAGGATCTCATGCCTGGCCTCACTGCAAAAGTGTTCCGTACATATAATGCTTCAATCACACTTGATGACATGTTGAATAGAGGAACCAAAGATGGAGACGTTGTAGAAAAGATTGCCACATACCAGCATGCAAACAAGGAGGTTGCAATTATTTGTAATCACCAGCGGACTGTTTCAAAATCACATGGGGCGCAAATGACGAGATTGAATGAAAAGATCTCTGAGCTCAGGGACATTGTGAAAGAGCTTAAAATTGATTTAGATAGAGCAAAGAAAGGGAAGCCCCCTTTAAAAGATGCTGATGGAAAGCGAAAGAGGAACTTGACCCCGGAAGCGTTAGAGAAGAAGATATCCCAAACGAATGCAAAGATTGAGAAGATAGAGCGAGATATGAAGACTAAAGAGGATTTGAAAACTGTAGCATTGGGCACGTCAAAGATCAACTACCTTGATCCTCGGATCACAGTCGCATGGTGTAAGCGTCATGAAGTCCCTATCGAAAAGATATTCAACAAGTCTCTTCTAGCAAAGTTTGCTTGGGCAATGGACGTCGATCCCGACTTCAGATTCTGATTGTTGTTTATATTCCTGGCGGTCTCAAACCCTAAATCATCATCCTTCGCCCCCGGCTCAACCTCGAAAGAAAATAAGAAAAAATGAAGAAGAGATGGAGTAGGAAAAATTGAACTGTACTATTTTGCATTACATTCAATTTTTTCTTTTTAATCCTTTAGTTTGCAGAGGAAAGTCCCATGATGTATGTTTATCTATCTAATTATATTTGGGGTAAAATGGCTCATTTCTGACACTCCCCATTCCCCATTCCCCTTCCCC

Coding sequence (CDS)

ATGGCTGTTGAGACTTTTGGAAAAGCTCCAATGTTTGATGACGATGACGATGAGGACAACATGCCGATAGTTTTTAAAAGGAGTAGTGCATCTAGGCAAAATCAGTTAGATTCGGAAATTAAGCATTCACAATCTCAGAGATTGAATAAGTTATCGGGGAGGCAGGGTTCTGATATATGTTCAACCAATGGCCAAAGCTCTAATAATCAAAAAAGGATGACTTCATTGCCGAAGACATCACCCATGAGATCCGAAATAGGAAGTCAGAAAGCATCCAAGTCTCCTATCCGGGAACCACCTGTTAAACCACTAGGCACATCTTTAAAAGTTTCAGATTCTATGGATAGCCGGTCGAAACCTTTGGTAAACACTGCTGTGAAAAAGGAGAATGTTTCTATAATTCTTACTCAAGAGAATAGTGACTCTGAAGACGATAAACCATTGAGTATTAGATTAAAAGGGAACTCCAGCCAGCCTAATAAAGGTCTTGTACCAACCGTACCAAAGAACAAAGTAAAAAAATCATCAGGAGATTCTGATGATGAAATTCCTTTGTCATCAAAATTTCAAATGAAATCTGGTTCAGGGGTGTTGGGTAGTAAACAACATGGTTTCGATGAGAAGAAACCATTGGTTCCAAGAGTACATCAGAATGGTTCGACCTCAAGGGATAAGCTGCAAAAGCCTGCTGTTCTTTCCAGCAAGAGACCTCTTCCTAATGCAGATTCCCATACCCCTTCCCAAATTTCAACAAAAAAGCCAAAGCTTTCAGATTCATCTACTCCTTTGAGTAGCAAGCTAGTATCATTGAAAGCAAAACAAGAAGTAGATGACGAGGACGACGATATTCCTATATCACAGAGAATGAAGAAGATGTCTGCACCAGTGAATAAGTCATCAAGCTCTAAGCAAACAGTATCAAAAGTTGTTTCTTCATCAATCAAGAAAACATTCAAAAAGTCCAAAAAGCCAGTCAAGAAATCTAAATATGTTAAGTCCACAAAGTTGCTGCCTAGTGCCGGTGATGGACAGAAAAAATGGACCACCTTGGTTCACAATGGTGTCATTTTCCCACCTCCATACCAGACTCACGGGGTGAAGATACTTTATAAAGGGAAGCCTGTTGATTTGACTCCGGAACAAGAGGAGGTTGCAACTATGTTTGCTGTGATGAAGGATACAGAATATATGCTAAAACCAAAATTCAAAGAAAATTTCTGGAATGACTGGCGCAAATTACTTGGGAAAAACCATGTCATTCAAAATTTGGATGATTGTGATTTCACCCCAATATATGACTGGTATCAGAAGGAAAAGGAGAAGAAGAAACAGATGACTACAGAAGAGAAGAAAGCTCTAAAGGAGGAGAAACTGAAACAAGAGGAGAAGTACATGTGGGCTATTCTTGATGGTGTTAAGGAGAAGGTTGGAAACTTTAGAGTTGAACCGCCTGGACTGTTCCGAGGCCGTGGAGAACATCCAAAGATGGGGAAATTGAAAAGGCGCATTCACCCCAGTGACATTACAATTAATATTGGAAAAGGTGCTCCAATCCCTGAGTGTCCTATACCTGGTGAAAGGTGGAAGGAAGTGAGACATGACAATACGGTGACATGGTTGGCTTTCTGGAATGATCCAATAAATCAAAAAGAATTCAAGTATGTGTTTCTGGCTGCTAGTAGTTCACTGAAAGGCCAGAGTGACAAAGAGAAATACGAGAAAGCAAGGATGCTCAAGGACTACATAAAAAACATAAGGAATGCATATACAAAGGATTTTACAAGCAAAGACTCTACACGAAGGCAAATTGCAGTTGCTACTTATCTCATTGATAAACTGGCTCTTAGGGCTGGGAATGAGAAAGATGACGATGAAGCTGATACAGTTGGCTGCTGTACACTGAAAGTAGAGAATGTTAAAGTGGTACCTCCGAATCTTCTGGAGTTCAACTTCCTCGGTAAAGATTCAATCAAGTATGAAAATACTGTTGAGGTTGAGCTCCCTGTTTTCAAAGCAATCACACAATTCCAAAGTGGAAAAAGTGGAACCGATGATCTGTTCGACAAGTTGGATACGAGTAAATTGAATGCGCATCTGAAGGATCTCATGCCTGGCCTCACTGCAAAAGTGTTCCGTACATATAATGCTTCAATCACACTTGATGACATGTTGAATAGAGGAACCAAAGATGGAGACGTTGTAGAAAAGATTGCCACATACCAGCATGCAAACAAGGAGGTTGCAATTATTTGTAATCACCAGCGGACTGTTTCAAAATCACATGGGGCGCAAATGACGAGATTGAATGAAAAGATCTCTGAGCTCAGGGACATTGTGAAAGAGCTTAAAATTGATTTAGATAGAGCAAAGAAAGGGAAGCCCCCTTTAAAAGATGCTGATGGAAAGCGAAAGAGGAACTTGACCCCGGAAGCGTTAGAGAAGAAGATATCCCAAACGAATGCAAAGATTGAGAAGATAGAGCGAGATATGAAGACTAAAGAGGATTTGAAAACTGTAGCATTGGGCACGTCAAAGATCAACTACCTTGATCCTCGGATCACAGTCGCATGGTGTAAGCGTCATGAAGTCCCTATCGAAAAGATATTCAACAAGTCTCTTCTAGCAAAGTTTGCTTGGGCAATGGACGTCGATCCCGACTTCAGATTCTGA

Protein sequence

MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRQGSDICSTNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSPIREPPVKPLGTSLKVSDSMDSRSKPLVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGNSSQPNKGLVPTVPKNKVKKSSGDSDDEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDKLQKPAVLSSKRPLPNADSHTPSQISTKKPKLSDSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKSSSSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHVIQNLDDCDFTPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPGLFRGRGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTRRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKYENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKDGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKELKIDLDRAKKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Homology
BLAST of Bhi11G000412 vs. TAIR 10
Match: AT5G55300.1 (DNA topoisomerase I alpha )

HSP 1 Score: 1023.8 bits (2646), Expect = 8.0e-299
Identity = 576/940 (61.28%), Postives = 689/940 (73.30%), Query Frame = 0

Query: 1   MAVETFGKAPMFDD--DDDEDNMPIVFKRSS--ASRQNQLDSEIKHSQSQRLNKLSGRQG 60
           M  ET  K P+ D+   D +D+ P+ FKR++  AS  NQ  S  + S++    K+S  + 
Sbjct: 1   MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPMR- 60

Query: 61  SDICSTNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSPIREPPVKPLGTSLKVSDSMDS 120
           S + S NG + +N        KTS ++S + S  +  SP + P    + +++K       
Sbjct: 61  SPVTSPNGTTPSN--------KTSIVKSSMPSSSSKASPAKSPLRNDMPSTVK------D 120

Query: 121 RSKPLVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGN----------SSQPNKGLVPT 180
           RS+      ++K+     +  E+ DSEDD+PLS  L GN          S QP K     
Sbjct: 121 RSQ------LQKDQSECKI--EHEDSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGD 180

Query: 181 VPKNKVKKSSGD-SDDEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDK 240
            P ++  +   D SDDE P+SS F+ K  SG+ G  Q   DEKKPLV ++HQNGST +++
Sbjct: 181 RPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVKNE 240

Query: 241 LQKPAVLSSKRPLPNADSHTPSQISTKKPKLSDSSTPLSSK--------------LVS-- 300
           +    VL  KRPL    S    Q S KK K+S S T +  K              LVS  
Sbjct: 241 VPNGKVL-GKRPLEKNSS--ADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVSPK 300

Query: 301 LKAKQ-------EVDDEDDDIPISQRMKKMSAPVNKSSSSKQTVS-------------KV 360
           +KAKQ         DD+DDD+PIS+R K  S+  N SS+  + V               V
Sbjct: 301 MKAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKARNV 360

Query: 361 VSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQTHGVKIL 420
           VS   +   K +KK  K SKY  S+K  PS+GDGQKKWTTLVHNGVIFPPPY+ HG+KIL
Sbjct: 361 VSPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGIKIL 420

Query: 421 YKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHVIQNLDDCDFT 480
           YKGKPVDLT EQEEVATMFAVM++T+Y  KP+F+ENFWNDWR+LLGK HVIQ LDDCDFT
Sbjct: 421 YKGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDCDFT 480

Query: 481 PIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPGLFRG 540
           PIY+W+ +EKEKKKQM+TEEKKALKEEK+KQEEKYMWA++DGVKEK+GNFRVEPPGLFRG
Sbjct: 481 PIYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPPGLFRG 540

Query: 541 RGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINQ 600
           RGEHPKMGKLK+RIHP +IT+NIGKGAPIPECPI GERWKEV+HDNTVTWLAFW DPIN 
Sbjct: 541 RGEHPKMGKLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADPINP 600

Query: 601 KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTRRQIAVATYL 660
           KEFKYVFL A SSLKG SDKEKYEKAR L D+I NIR  YTK+FT+KD   RQIAVATYL
Sbjct: 601 KEFKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVATYL 660

Query: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKYENTVEVELPV 720
           IDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE  V
Sbjct: 661 IDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLV 720

Query: 721 FKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780
           +KAI QFQ+GKS TDDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TK
Sbjct: 721 YKAIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETK 780

Query: 781 DGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKELKIDLDRA 840
           DGDV +KI  YQ ANKEVAIICNHQRTVSK+HGAQ+ +L  +I EL++++KELK +LDRA
Sbjct: 781 DGDVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRA 840

Query: 841 KKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL 890
           KKGKPPL+ +DGK+ R+L P A EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYL
Sbjct: 841 KKGKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYL 900

BLAST of Bhi11G000412 vs. TAIR 10
Match: AT5G55300.2 (DNA topoisomerase I alpha )

HSP 1 Score: 1018.8 bits (2633), Expect = 2.6e-297
Identity = 574/937 (61.26%), Postives = 686/937 (73.21%), Query Frame = 0

Query: 1   MAVETFGKAPMFDD--DDDEDNMPIVFKRSS--ASRQNQLDSEIKHSQSQRLNKLSGRQG 60
           M  ET  K P+ D+   D +D+ P+ FKR++  AS  NQ  S  + S++    K+S  + 
Sbjct: 1   MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPMR- 60

Query: 61  SDICSTNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSPIREPPVKPLGTSLKVSDSMDS 120
           S + S NG + +N        KTS ++S + S  +  SP + P    + +++K       
Sbjct: 61  SPVTSPNGTTPSN--------KTSIVKSSMPSSSSKASPAKSPLRNDMPSTVK------D 120

Query: 121 RSKPLVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGN----------SSQPNKGLVPT 180
           RS+      ++K+     +  E+ DSEDD+PLS  L GN          S QP K     
Sbjct: 121 RSQ------LQKDQSECKI--EHEDSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGD 180

Query: 181 VPKNKVKKSSGD-SDDEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDK 240
            P ++  +   D SDDE P+SS F+ K  SG+ G  Q   DEKKPLV ++HQNGST +++
Sbjct: 181 RPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVKNE 240

Query: 241 LQKPAVLSSKRPLPNADSHTPSQISTKKPKLSDSSTPLSSK--------------LVS-- 300
           +    VL  KRPL    S    Q S KK K+S S T +  K              LVS  
Sbjct: 241 VPNGKVL-GKRPLEKNSS--ADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVSPK 300

Query: 301 LKAKQ-------EVDDEDDDIPISQRMKKMSAPVNKSSSSKQTVS-------------KV 360
           +KAKQ         DD+DDD+PIS+R K  S+  N SS+  + V               V
Sbjct: 301 MKAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKARNV 360

Query: 361 VSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQTHGVKIL 420
           VS   +   K +KK  K SKY  S+K  PS+GDGQKKWTTLVHNGVIFPPPY+ HG+KIL
Sbjct: 361 VSPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGIKIL 420

Query: 421 YKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHVIQNLDDCDFT 480
           YKGKPVDLT EQEEVATMFAVM++T+Y  KP+F+ENFWNDWR+LLGK HVIQ LDDCDFT
Sbjct: 421 YKGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDCDFT 480

Query: 481 PIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPGLFRG 540
           PIY+W+ +EKEKKKQM+TEEKKALKEEK+KQEEKYMWA++DGVKEK+GNFRVEPPGLFRG
Sbjct: 481 PIYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPPGLFRG 540

Query: 541 RGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINQ 600
           RGEHPKMGKLK+RIHP +IT+NIGKGAPIPECPI GERWKEV+HDNTVTWLAFW DPIN 
Sbjct: 541 RGEHPKMGKLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADPINP 600

Query: 601 KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTRRQIAVATYL 660
           KEFKYVFL A SSLKG SDKEKYEKAR L D+I NIR  YTK+FT+KD   RQIAVATYL
Sbjct: 601 KEFKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVATYL 660

Query: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKYENTVEVELPV 720
           IDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE  V
Sbjct: 661 IDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLV 720

Query: 721 FKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780
           +KAI QFQ+GKS TDDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TK
Sbjct: 721 YKAIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETK 780

Query: 781 DGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKELKIDLDRA 840
           DGDV +KI  YQ ANKEVAIICNHQRTVSK+HGAQ+ +L  +I EL++++KELK +LDRA
Sbjct: 781 DGDVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRA 840

Query: 841 KKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL 887
           KKGKPPL+ +DGK+ R+L P A EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYL
Sbjct: 841 KKGKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYL 900

BLAST of Bhi11G000412 vs. TAIR 10
Match: AT5G55310.1 (DNA topoisomerase 1 beta )

HSP 1 Score: 1008.1 bits (2605), Expect = 4.5e-294
Identity = 573/941 (60.89%), Postives = 676/941 (71.84%), Query Frame = 0

Query: 1   MAVETFGKAPMFD-----DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRQ 60
           MA E F K  + +     +D+DED++P+VFKR+S       ++    ++   +N  +  +
Sbjct: 1   MATEAFVKPVVPNGHDGYEDEDEDDIPLVFKRNS-------NTAATTNRPSPIN--NAMR 60

Query: 61  GSDICSTNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSPIREPPVKPLGTSLKVSDSMD 120
            S I ST              P TSP RS   S ++S      P    +  S   S   D
Sbjct: 61  NSAIGSTKSSPPMRS------PLTSPNRSASSSTRSSMMKPALPSSSSVQRSTLKSPLRD 120

Query: 121 SRSKPLVNTAVKKEN----VSIILTQENSDSEDDKPLSIRLKGNS-------SQPNKGLV 180
            RS        K+ N       +   ++ DSEDDKPLS RLK +S       S   +G  
Sbjct: 121 DRS-----VVAKERNGFGKAPSVSKSDDEDSEDDKPLSARLKLDSKEVTKQPSSSGRGST 180

Query: 181 PTVPKNKVKKSSGDS--------DDEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVH 240
               +    +  G S        D+  P+SS  Q K+  G   SK    ++K+PLV  + 
Sbjct: 181 QQAVQKSNMRPQGLSDYTKKKVLDERAPMSSTVQTKTSVGTSSSKPVHIEQKRPLVNNID 240

Query: 241 QNG---STSRDKLQKPAVLSSKRPLPNADSHTPSQISTKKPKLS---------------- 300
           +NG    T     Q PA    KRPL    S   +Q S K+PKLS                
Sbjct: 241 RNGLKPKTEGHSSQAPA----KRPLEKGSS--SNQSSVKRPKLSEPARPVKVEQGSHISA 300

Query: 301 ---------DSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKSSSSKQTVSK 360
                    D+S PL +   ++  K++  D DD +PI+ RMK  S+  NKSSS+K + SK
Sbjct: 301 TQDAKGKNLDASKPLRANQATV--KEDNSDGDDHVPIASRMKSDSSN-NKSSSAKPSSSK 360

Query: 361 VVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQTHGVKI 420
           +++SS +   +K  K VK SKY KS+K LPS GDGQKKW TL HNGVIFPPPY+ HGVKI
Sbjct: 361 MIASSSRTIAQKPNKWVKDSKYSKSSKSLPS-GDGQKKWKTLQHNGVIFPPPYKRHGVKI 420

Query: 421 LYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHVIQNLDDCDF 480
           L++GKPVDLTPEQEEVATMFAVM++TEY  KPKF+ENFWNDWRKLLGKNH+I++LDDCDF
Sbjct: 421 LFQGKPVDLTPEQEEVATMFAVMRETEYYKKPKFRENFWNDWRKLLGKNHMIKSLDDCDF 480

Query: 481 TPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPGLFR 540
           TPIY+WY +EKE KKQMT EEK+ +KEEKLKQEEKYMWA+LDGV+E++GNFRVEPPGLFR
Sbjct: 481 TPIYEWYMQEKETKKQMTAEEKRIVKEEKLKQEEKYMWAVLDGVRERIGNFRVEPPGLFR 540

Query: 541 GRGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFWNDPIN 600
           GRGEHPKMGKLK+RI P DITINIGK APIPECPIPGERWKEV+HDNTVTWLAFW+DPIN
Sbjct: 541 GRGEHPKMGKLKKRIRPCDITINIGKEAPIPECPIPGERWKEVKHDNTVTWLAFWSDPIN 600

Query: 601 QKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTRRQIAVATY 660
            KEFKYVFLAASSSLKGQSDKEKYEKAR L ++I +IR AYTKDF +KD T+RQIAVATY
Sbjct: 601 PKEFKYVFLAASSSLKGQSDKEKYEKARKLHNHIGSIRAAYTKDFNNKDVTKRQIAVATY 660

Query: 661 LIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKYENTVEVELP 720
           LIDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN L+F+FLGKDSI+Y NTVEVE  
Sbjct: 661 LIDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKLKFDFLGKDSIQYVNTVEVEPL 720

Query: 721 VFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGT 780
           V+KAI QFQ+GKS TDDLFD+LDTSKLN HLK+LM GLTAKVFRTYNASITLD ML++ T
Sbjct: 721 VYKAIGQFQAGKSKTDDLFDELDTSKLNTHLKELMAGLTAKVFRTYNASITLDLMLSKET 780

Query: 781 KDGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKELKIDLDR 840
           +DGDV EK+  YQ ANKEVAIICNHQRTVSKSHGAQ+ +L  KI ELR+ +KEL IDLDR
Sbjct: 781 RDGDVPEKVVVYQQANKEVAIICNHQRTVSKSHGAQVEKLAVKIEELREQIKELNIDLDR 840

Query: 841 AKKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINY 890
           AKKG+ PL  +DGKRKRNLTPEALEKKI QT  KIEK+ERDM+TKED+KTVALGTSKINY
Sbjct: 841 AKKGRTPLMGSDGKRKRNLTPEALEKKIMQTQGKIEKMERDMQTKEDMKTVALGTSKINY 900

BLAST of Bhi11G000412 vs. TAIR 10
Match: AT5G55300.3 (DNA topoisomerase I alpha )

HSP 1 Score: 423.3 bits (1087), Expect = 4.8e-118
Identity = 207/271 (76.38%), Postives = 243/271 (89.67%), Query Frame = 0

Query: 619 NEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKYENTVEVELPVFKAITQFQS 678
           ++ DDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE  V+KAI QFQ+
Sbjct: 291 DDDDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLVYKAIGQFQA 350

Query: 679 GKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKDGDVVEKIA 738
           GKS TDDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TKDGDV +KI 
Sbjct: 351 GKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETKDGDVTQKIV 410

Query: 739 TYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKELKIDLDRAKKGKPPLKD 798
            YQ ANKEVAIICNHQRTVSK+HGAQ+ +L  +I EL++++KELK +LDRAKKGKPPL+ 
Sbjct: 411 VYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRAKKGKPPLEG 470

Query: 799 ADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAWC 858
           +DGK+ R+L P A EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYLDPRITVAWC
Sbjct: 471 SDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYLDPRITVAWC 530

Query: 859 KRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 890
           KRHEVPIEKIF KSLL KFAWAMDV+P++RF
Sbjct: 531 KRHEVPIEKIFTKSLLEKFAWAMDVEPEYRF 561


HSP 2 Score: 93.6 bits (231), Expect = 8.6e-19
Identity = 104/305 (34.10%), Postives = 157/305 (51.48%), Query Frame = 0

Query: 1   MAVETFGKAPMFDD--DDDEDNMPIVFKRSS--ASRQNQLDSEIKHSQSQRLNKLSGRQG 60
           M  ET  K P+ D+   D +D+ P+ FKR++  AS  NQ  S  + S++    K+S  + 
Sbjct: 1   MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPMR- 60

Query: 61  SDICSTNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSPIREPPVKPLGTSLKVSDSMDS 120
           S + S NG + +N        KTS ++S + S  +  SP + P    + +++K       
Sbjct: 61  SPVTSPNGTTPSN--------KTSIVKSSMPSSSSKASPAKSPLRNDMPSTVK------D 120

Query: 121 RSKPLVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGN----------SSQPNKGLVPT 180
           RS+      ++K+     +  E+ DSEDD+PLS  L GN          S QP K     
Sbjct: 121 RSQ------LQKDQSECKI--EHEDSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGD 180

Query: 181 VPKNKVKKSSGD-SDDEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDK 240
            P ++  +   D SDDE P+SS F+ K  SG+ G  Q   DEKKPLV ++HQNGST +++
Sbjct: 181 RPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVKNE 240

Query: 241 LQKPAVLSSKRPLPNADSHTPSQISTKKPKLSDSSTPLSSKLVSLKAKQEVDDEDDDIPI 291
           +    VL  KRPL    S    Q S KK K+  S++P S K+     K+E+DD+   + +
Sbjct: 241 VPNGKVL-GKRPLEKNSS--ADQSSLKKAKI--SASPTSVKMKQDSVKKEIDDK-GRVLV 275

BLAST of Bhi11G000412 vs. TAIR 10
Match: AT4G26701.1 (DNA binding;DNA topoisomerase type Is )

HSP 1 Score: 129.0 bits (323), Expect = 1.9e-29
Identity = 60/68 (88.24%), Postives = 64/68 (94.12%), Query Frame = 0

Query: 822 KIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAM 881
           KIEK+ERDM+TKEDLKTVALGTSKINY+DPRITVAWCKRHE PIEKIFNKSLL KFAWAM
Sbjct: 2   KIEKMERDMQTKEDLKTVALGTSKINYMDPRITVAWCKRHEAPIEKIFNKSLLEKFAWAM 61

Query: 882 DVDPDFRF 890
           DV+P F F
Sbjct: 62  DVEPHFTF 69

BLAST of Bhi11G000412 vs. ExPASy Swiss-Prot
Match: P30181 (DNA topoisomerase 1 alpha OS=Arabidopsis thaliana OX=3702 GN=TOP1A PE=1 SV=1)

HSP 1 Score: 1023.8 bits (2646), Expect = 1.1e-297
Identity = 576/940 (61.28%), Postives = 689/940 (73.30%), Query Frame = 0

Query: 1   MAVETFGKAPMFDD--DDDEDNMPIVFKRSS--ASRQNQLDSEIKHSQSQRLNKLSGRQG 60
           M  ET  K P+ D+   D +D+ P+ FKR++  AS  NQ  S  + S++    K+S  + 
Sbjct: 1   MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPMR- 60

Query: 61  SDICSTNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSPIREPPVKPLGTSLKVSDSMDS 120
           S + S NG + +N        KTS ++S + S  +  SP + P    + +++K       
Sbjct: 61  SPVTSPNGTTPSN--------KTSIVKSSMPSSSSKASPAKSPLRNDMPSTVK------D 120

Query: 121 RSKPLVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGN----------SSQPNKGLVPT 180
           RS+      ++K+     +  E+ DSEDD+PLS  L GN          S QP K     
Sbjct: 121 RSQ------LQKDQSECKI--EHEDSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGD 180

Query: 181 VPKNKVKKSSGD-SDDEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDK 240
            P ++  +   D SDDE P+SS F+ K  SG+ G  Q   DEKKPLV ++HQNGST +++
Sbjct: 181 RPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVKNE 240

Query: 241 LQKPAVLSSKRPLPNADSHTPSQISTKKPKLSDSSTPLSSK--------------LVS-- 300
           +    VL  KRPL    S    Q S KK K+S S T +  K              LVS  
Sbjct: 241 VPNGKVL-GKRPLEKNSS--ADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVSPK 300

Query: 301 LKAKQ-------EVDDEDDDIPISQRMKKMSAPVNKSSSSKQTVS-------------KV 360
           +KAKQ         DD+DDD+PIS+R K  S+  N SS+  + V               V
Sbjct: 301 MKAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKARNV 360

Query: 361 VSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQTHGVKIL 420
           VS   +   K +KK  K SKY  S+K  PS+GDGQKKWTTLVHNGVIFPPPY+ HG+KIL
Sbjct: 361 VSPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGIKIL 420

Query: 421 YKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHVIQNLDDCDFT 480
           YKGKPVDLT EQEEVATMFAVM++T+Y  KP+F+ENFWNDWR+LLGK HVIQ LDDCDFT
Sbjct: 421 YKGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDCDFT 480

Query: 481 PIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPGLFRG 540
           PIY+W+ +EKEKKKQM+TEEKKALKEEK+KQEEKYMWA++DGVKEK+GNFRVEPPGLFRG
Sbjct: 481 PIYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPPGLFRG 540

Query: 541 RGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINQ 600
           RGEHPKMGKLK+RIHP +IT+NIGKGAPIPECPI GERWKEV+HDNTVTWLAFW DPIN 
Sbjct: 541 RGEHPKMGKLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADPINP 600

Query: 601 KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTRRQIAVATYL 660
           KEFKYVFL A SSLKG SDKEKYEKAR L D+I NIR  YTK+FT+KD   RQIAVATYL
Sbjct: 601 KEFKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVATYL 660

Query: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKYENTVEVELPV 720
           IDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE  V
Sbjct: 661 IDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLV 720

Query: 721 FKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780
           +KAI QFQ+GKS TDDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TK
Sbjct: 721 YKAIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETK 780

Query: 781 DGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKELKIDLDRA 840
           DGDV +KI  YQ ANKEVAIICNHQRTVSK+HGAQ+ +L  +I EL++++KELK +LDRA
Sbjct: 781 DGDVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRA 840

Query: 841 KKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL 890
           KKGKPPL+ +DGK+ R+L P A EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYL
Sbjct: 841 KKGKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYL 900

BLAST of Bhi11G000412 vs. ExPASy Swiss-Prot
Match: Q9FJ79 (DNA topoisomerase 1 beta OS=Arabidopsis thaliana OX=3702 GN=TOP1B PE=1 SV=1)

HSP 1 Score: 1008.1 bits (2605), Expect = 6.4e-293
Identity = 573/941 (60.89%), Postives = 676/941 (71.84%), Query Frame = 0

Query: 1   MAVETFGKAPMFD-----DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRQ 60
           MA E F K  + +     +D+DED++P+VFKR+S       ++    ++   +N  +  +
Sbjct: 1   MATEAFVKPVVPNGHDGYEDEDEDDIPLVFKRNS-------NTAATTNRPSPIN--NAMR 60

Query: 61  GSDICSTNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSPIREPPVKPLGTSLKVSDSMD 120
            S I ST              P TSP RS   S ++S      P    +  S   S   D
Sbjct: 61  NSAIGSTKSSPPMRS------PLTSPNRSASSSTRSSMMKPALPSSSSVQRSTLKSPLRD 120

Query: 121 SRSKPLVNTAVKKEN----VSIILTQENSDSEDDKPLSIRLKGNS-------SQPNKGLV 180
            RS        K+ N       +   ++ DSEDDKPLS RLK +S       S   +G  
Sbjct: 121 DRS-----VVAKERNGFGKAPSVSKSDDEDSEDDKPLSARLKLDSKEVTKQPSSSGRGST 180

Query: 181 PTVPKNKVKKSSGDS--------DDEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVH 240
               +    +  G S        D+  P+SS  Q K+  G   SK    ++K+PLV  + 
Sbjct: 181 QQAVQKSNMRPQGLSDYTKKKVLDERAPMSSTVQTKTSVGTSSSKPVHIEQKRPLVNNID 240

Query: 241 QNG---STSRDKLQKPAVLSSKRPLPNADSHTPSQISTKKPKLS---------------- 300
           +NG    T     Q PA    KRPL    S   +Q S K+PKLS                
Sbjct: 241 RNGLKPKTEGHSSQAPA----KRPLEKGSS--SNQSSVKRPKLSEPARPVKVEQGSHISA 300

Query: 301 ---------DSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKSSSSKQTVSK 360
                    D+S PL +   ++  K++  D DD +PI+ RMK  S+  NKSSS+K + SK
Sbjct: 301 TQDAKGKNLDASKPLRANQATV--KEDNSDGDDHVPIASRMKSDSSN-NKSSSAKPSSSK 360

Query: 361 VVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQTHGVKI 420
           +++SS +   +K  K VK SKY KS+K LPS GDGQKKW TL HNGVIFPPPY+ HGVKI
Sbjct: 361 MIASSSRTIAQKPNKWVKDSKYSKSSKSLPS-GDGQKKWKTLQHNGVIFPPPYKRHGVKI 420

Query: 421 LYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHVIQNLDDCDF 480
           L++GKPVDLTPEQEEVATMFAVM++TEY  KPKF+ENFWNDWRKLLGKNH+I++LDDCDF
Sbjct: 421 LFQGKPVDLTPEQEEVATMFAVMRETEYYKKPKFRENFWNDWRKLLGKNHMIKSLDDCDF 480

Query: 481 TPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPGLFR 540
           TPIY+WY +EKE KKQMT EEK+ +KEEKLKQEEKYMWA+LDGV+E++GNFRVEPPGLFR
Sbjct: 481 TPIYEWYMQEKETKKQMTAEEKRIVKEEKLKQEEKYMWAVLDGVRERIGNFRVEPPGLFR 540

Query: 541 GRGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFWNDPIN 600
           GRGEHPKMGKLK+RI P DITINIGK APIPECPIPGERWKEV+HDNTVTWLAFW+DPIN
Sbjct: 541 GRGEHPKMGKLKKRIRPCDITINIGKEAPIPECPIPGERWKEVKHDNTVTWLAFWSDPIN 600

Query: 601 QKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTRRQIAVATY 660
            KEFKYVFLAASSSLKGQSDKEKYEKAR L ++I +IR AYTKDF +KD T+RQIAVATY
Sbjct: 601 PKEFKYVFLAASSSLKGQSDKEKYEKARKLHNHIGSIRAAYTKDFNNKDVTKRQIAVATY 660

Query: 661 LIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKYENTVEVELP 720
           LIDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN L+F+FLGKDSI+Y NTVEVE  
Sbjct: 661 LIDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKLKFDFLGKDSIQYVNTVEVEPL 720

Query: 721 VFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGT 780
           V+KAI QFQ+GKS TDDLFD+LDTSKLN HLK+LM GLTAKVFRTYNASITLD ML++ T
Sbjct: 721 VYKAIGQFQAGKSKTDDLFDELDTSKLNTHLKELMAGLTAKVFRTYNASITLDLMLSKET 780

Query: 781 KDGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKELKIDLDR 840
           +DGDV EK+  YQ ANKEVAIICNHQRTVSKSHGAQ+ +L  KI ELR+ +KEL IDLDR
Sbjct: 781 RDGDVPEKVVVYQQANKEVAIICNHQRTVSKSHGAQVEKLAVKIEELREQIKELNIDLDR 840

Query: 841 AKKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINY 890
           AKKG+ PL  +DGKRKRNLTPEALEKKI QT  KIEK+ERDM+TKED+KTVALGTSKINY
Sbjct: 841 AKKGRTPLMGSDGKRKRNLTPEALEKKIMQTQGKIEKMERDMQTKEDMKTVALGTSKINY 900

BLAST of Bhi11G000412 vs. ExPASy Swiss-Prot
Match: P93119 (DNA topoisomerase 1 OS=Daucus carota OX=4039 GN=TOP1 PE=2 SV=1)

HSP 1 Score: 840.9 bits (2171), Expect = 1.3e-242
Identity = 483/793 (60.91%), Postives = 575/793 (72.51%), Query Frame = 0

Query: 139 NSDSEDDKPLSIR-----LKGNSSQPNKG------LVPTVPKNKVKKSSGDSDDEIPLSS 198
           + DS+DDKPLS +     L GNS     G         T PK ++ K     +DE+ LSS
Sbjct: 32  SEDSDDDKPLSHKLSSGALNGNSHHIRMGSNLSCPSPYTSPKPRIIKG---PEDEMLLSS 91

Query: 199 KFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDKLQKPAVLSSKRPLPNADSHTPS 258
           + Q+ +G+    ++    DE KPL             +L+ P+   +KRPL    +  PS
Sbjct: 92  ELQLNAGAS--NAESSDSDESKPLA-----------SELEDPSSSLNKRPLIQPKNSDPS 151

Query: 259 QISTKKPKLSDSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKSSSSKQTVS 318
              +KK KLS+ S P + KL +  A+QE    DDD  IS R KK + P +K S  K+   
Sbjct: 152 --PSKKAKLSEPSAPTNRKLKT--AEQEEAAADDDPSISNRNKKSTTPASKVSDKKKRPD 211

Query: 319 KVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQTHGVK 378
             VS+S+ K        V  SK +K   +L    +GQ     LVHNGVIFPP Y+ HGVK
Sbjct: 212 --VSASVNK--------VDFSKSLKVPLVLVKGRNGQ----PLVHNGVIFPPLYKPHGVK 271

Query: 379 ILYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHVIQNLDDCD 438
           +LY+  PVDLTPEQEEVATMFAVM +TEYM KPKF+ENF +DWRK+LG+ H+IQNL+DCD
Sbjct: 272 MLYRENPVDLTPEQEEVATMFAVMLETEYMTKPKFRENFMSDWRKILGEKHIIQNLEDCD 331

Query: 439 FTPIYDWYQKEKEKKKQMTTEEKKALKEEKLK-QEEKYMWAILDGV-KEKVGNFRVEPPG 498
           FTPIY+W+Q+EKEKKKQM+T+EKKA+KE K + ++EKYMWA++DG+ +EKVGNFRV    
Sbjct: 332 FTPIYEWHQREKEKKKQMSTDEKKAIKEGKDETRKEKYMWAVVDGMSREKVGNFRVGTTR 391

Query: 499 LFRGRGEHPKMGKLKRRIHPS-DITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFWN 558
           + +      K  ++K+ ++   DITINIGK APIPE PIPGERWKE+RHDNTVTWLAFWN
Sbjct: 392 VVQRSWRASKDPQVKKNVYSQIDITINIGKDAPIPEPPIPGERWKEIRHDNTVTWLAFWN 451

Query: 559 DPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIR-NAYTKDFTSKDSTRRQI 618
           DPIN KEFKYVFLAASSSLKG+  +EKYEK+R LKDYI+ IR  AYTKDF SKDS +RQI
Sbjct: 452 DPINPKEFKYVFLAASSSLKGKVTREKYEKSRKLKDYIEGIRAAAYTKDFASKDSKKRQI 511

Query: 619 AVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKYENTV 678
           AVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENV+   PN+L+F+FLGKDSI+Y+N V
Sbjct: 512 AVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVETKRPNILKFDFLGKDSIRYQNEV 571

Query: 679 EVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDM 738
           EVE  VF AI QF+SGK G DDLFD+LDTSKLNAHLK+LMPGLTAKVFRTYNASITLD+M
Sbjct: 572 EVEPRVFSAIEQFRSGKEGGDDLFDQLDTSKLNAHLKELMPGLTAKVFRTYNASITLDEM 631

Query: 739 -----LNRGTKDGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI 798
                L+R TK GDV EK+  YQHANKEVAIICNHQRTVSKSH AQM RLNEKI EL+ +
Sbjct: 632 VRCKLLSRETKGGDVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQMVRLNEKIEELKGL 691

Query: 799 VKELKIDLDRAKKGKPPLKDADGKRKRNLTPE----------------------ALEKKI 858
           +KEL+ DL R  KGKPPLK++DGK KRNL  +                      ++ KKI
Sbjct: 692 LKELQEDLTRVNKGKPPLKNSDGKPKRNLNLKRYKGKLLKLIQKLRKWSETRRLSVTKKI 751

Query: 859 SQTNAKIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 890
           +QTN KIEK+ERD +TKEDLKTVALGTSKINYLDPRITVAWCKRH+VPIEKIFNKSLLAK
Sbjct: 752 AQTNTKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHDVPIEKIFNKSLLAK 790

BLAST of Bhi11G000412 vs. ExPASy Swiss-Prot
Match: Q54RC3 (DNA topoisomerase 1 OS=Dictyostelium discoideum OX=44689 GN=top1 PE=3 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 3.3e-148
Identity = 349/867 (40.25%), Postives = 501/867 (57.79%), Query Frame = 0

Query: 71  KRMTSLPKTSPMRSEIGSQKASKSPIREPPVKPLGTSLKVSDSMDSRSKPLVNTAVKKEN 130
           K++T  PK +P+  +  +    K PI  P  KP+ +S   SDS DS S    + +   ++
Sbjct: 59  KKLTPTPKPAPVTPKPTAAVNGKKPI--PKKKPVDSS-SDSDSSDSSSD---SDSDSSDS 118

Query: 131 VSIILTQENSDSEDDKPLSIRLKGNSSQPNK--GLVPTVPKNKVKKSSGDSDDEIPLSSK 190
            S   + ++S S+ D P   + K  S  PNK  G V    KN  K ++  +     + SK
Sbjct: 119 DSDSSSSDSSSSDSDVP---KKKPISKIPNKPSGTVSKTSKNSPKTTTTTTTTTTTVKSK 178

Query: 191 FQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDKLQKPAVLSSKRPLPNADSHTPSQ 250
              K       S     D           + S S  + +KP    SK+P     +    +
Sbjct: 179 PAPKVKPEQSSSDDSSSDSSD---DSSDDSSSDSSSEEEKPKKKPSKKP-----AAVKKE 238

Query: 251 ISTKKPKLSDSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKSSSSKQTVSK 310
           I+ KK       T  ++K   +K + EV  E      ++++KK  +  +     K T  K
Sbjct: 239 ITVKK------ETTTTTKKTPVKKEVEVKKE-TKTTTTKKVKKEESD-DDGEDEKTTTKK 298

Query: 311 VVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQTHGVKI 370
             ++  +K  ++ +   ++ + V          D   KWTTL+HNG +FPP Y  HG+K 
Sbjct: 299 KTTTRKRKLKEEEEGEEEEEEGVNKWWDKEDDEDDGTKWTTLIHNGPVFPPAYVPHGIKF 358

Query: 371 LYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLL--GKNHVIQNLDDC 430
           +Y GK V LTP+QEEVAT +A   +T+++ K  FK+NF+ +++ LL   +  +++ +D C
Sbjct: 359 MYDGKVVKLTPKQEEVATFYANYLETDHVKKQAFKDNFFKEFKGLLTSEQKEIVKKMDKC 418

Query: 431 DFTPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPGL 490
           DF+ I+   + +KE++K  + EEK A   EK K +EK+ +A +DG KEKVGN+ +EPPGL
Sbjct: 419 DFSHIHTHLKTKKEQRKARSKEEKDAETAEKKKIQEKHGFATIDGFKEKVGNYMIEPPGL 478

Query: 491 FRGRGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFWNDP 550
           F GRG+HPK G LK+RI PSD+T+NIG+G  IP  PIPGE+WKE+ H+NTVTWLAFW + 
Sbjct: 479 FLGRGQHPKTGMLKQRIMPSDVTLNIGEGEVIPPSPIPGEKWKEIVHNNTVTWLAFWRES 538

Query: 551 INQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTRRQIAVA 610
           +N   FKYV+LA SS +KGQ+D++K+E AR LK+ I +IR  Y K F  + + +RQ+AVA
Sbjct: 539 VN-GGFKYVWLAPSSKIKGQADRKKFEVARGLKNCIGDIRKGYQKSFNDESTEKRQLAVA 598

Query: 611 TYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKYENTVEVE 670
            YLID LALR GNEK +D+ADTVGCC+L+VE++K+   N +  +FLGKDS++Y NTV++ 
Sbjct: 599 LYLIDFLALRVGNEKGEDQADTVGCCSLRVEHIKLESNNTITLDFLGKDSMRYLNTVQIR 658

Query: 671 LPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNR 730
              +K +  F  GK  TD LFD L  ++LN HLK  M GL+AKVFRTYNASITL   L++
Sbjct: 659 EDAYKVLKTFVKGKKPTDLLFDSLTVTQLNNHLKKQMTGLSAKVFRTYNASITLQKELDK 718

Query: 731 -GTKD-GDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKELKI 790
             TK+   + EKI  Y   N+EVAI+CNHQ+   K+    M++++EKI ELRD+      
Sbjct: 719 MDTKEFQSIDEKILFYNRCNREVAILCNHQKAPPKNLAETMSKIDEKIQELRDLQ----- 778

Query: 791 DLDRAKKGKPP-------------------------LKDADGKR--------------KR 850
           DL R K GK P                          KD D ++              K 
Sbjct: 779 DLCRIKIGKTPKSQQEDIESREAERLPKLIQLAITNKKDKDKEKTDKEISFDVKAAFEKS 838

Query: 851 NLTPEA---LEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHE 890
              P+A   +++KI++   +I+K E     K++LKTVALGTSKINYLDPRITVAWCK+++
Sbjct: 839 RTLPDAKGKIKEKIAKYEEQIKKQEILKTGKDELKTVALGTSKINYLDPRITVAWCKKNK 893

BLAST of Bhi11G000412 vs. ExPASy Swiss-Prot
Match: P07799 (DNA topoisomerase 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=top1 PE=1 SV=2)

HSP 1 Score: 523.9 bits (1348), Expect = 3.6e-147
Identity = 341/846 (40.31%), Postives = 472/846 (55.79%), Query Frame = 0

Query: 129 ENVSIILTQENSDSEDDKPLSIRLKGNSSQPNKGLVPTVPKNKVKKSSGDSDDEIPL--- 188
           + +S+  + E SDS ++ PLS  L                 NK  KS  D DD IP+   
Sbjct: 23  KRISMKESDEESDSSENHPLSESL-----------------NKKSKSESDEDD-IPIRKR 82

Query: 189 --SSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDKLQKPAVLSSKRPLPNADS 248
             SSK  M + S    +K  G             NG     K  K AV+  +        
Sbjct: 83  RASSKKNMSNSSSKKRAKVMG-------------NGGLKNGK--KTAVVKEEEDF----- 142

Query: 249 HTPSQISTKKPKLSDSSTPLSSKLVSLKA-KQEVDDEDDDIPISQRMKKMSAPVNKSSSS 308
              ++I+   PK    S    SK  +  A K+E  D DD +P+         P       
Sbjct: 143 ---NEIAKPSPKHKRVSKANGSKNGAKSAVKKEESDTDDSVPLRAVSTVSLTPY------ 202

Query: 309 KQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQ 368
           K  +    S++  ++    +   +  K+  S  +     D  +KWTTL HNGVIF PPY+
Sbjct: 203 KSELPSGASTTQNRSPNDEEDEDEDYKWWTSENI-----DDTQKWTTLEHNGVIFAPPYE 262

Query: 369 --THGVKILYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGK---N 428
                VK++Y G PV+L PE EEVA  +A M +T++   P F++NF+ D+ K+  +   N
Sbjct: 263 PLPKNVKLIYDGNPVNLPPEAEEVAGFYAAMLETDHAKNPVFQDNFFRDFLKVCDECNFN 322

Query: 429 HVIQNLDDCDFTPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVG 488
           H I+    CDFT ++  +++++E+KK M  E+KKA+K++K ++EEKY W ILDG KEKVG
Sbjct: 323 HNIKEFSKCDFTQMFHHFEQKREEKKSMPKEQKKAIKQKKDEEEEKYKWCILDGRKEKVG 382

Query: 489 NFRVEPPGLFRGRGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTV 548
           NFR+EPPGLFRGRG HPK G LKRR++P  ITINIG+G P+PE P+PG +W EV+HDNTV
Sbjct: 383 NFRIEPPGLFRGRGSHPKTGSLKRRVYPEQITINIGEGVPVPE-PLPGHQWAEVKHDNTV 442

Query: 549 TWLAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKD 608
           TWLA W++ IN    KYVFLAA SSLKGQSD +KYEK+R LKDYI +IR  Y KD  S+ 
Sbjct: 443 TWLATWHENIN-NNVKYVFLAAGSSLKGQSDLKKYEKSRKLKDYIDDIRKGYRKDLKSEL 502

Query: 609 STRRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSI 668
           +  RQ   A YLID  ALRAGNEK +DEADTVGCC+L+ E+V + PP  + F+FLGKDSI
Sbjct: 503 TVERQRGTAMYLIDVFALRAGNEKGEDEADTVGCCSLRYEHVTLKPPRTVVFDFLGKDSI 562

Query: 669 KYENTVEVELPVFKAITQF-QSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNA 728
           +Y N VEV+  VFK +  F +  K   D +FD+L T+ LN +L  LM GL+AKVFRTYNA
Sbjct: 563 RYYNEVEVDPQVFKNLKIFKRPPKKEGDLIFDRLSTNSLNKYLTSLMDGLSAKVFRTYNA 622

Query: 729 SITLDDMLNRGTKDGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISEL- 788
           S T+ + L +  K+  + +KI  Y  AN+ VAI+CNHQR+V+K+H  QM R  E+I  L 
Sbjct: 623 SYTMAEELKKMPKNLTLADKILFYNRANRTVAILCNHQRSVTKNHDVQMERFAERIKALQ 682

Query: 789 --RDIVKELKIDLD-RAKKGKPPL------------------------------------ 848
             R  ++++ ++L+ +  K KP L                                    
Sbjct: 683 YQRMRLRKMMLNLEPKLAKSKPELLAKEEGITDSWIVKHHETLYELEKEKIKKKFDRENE 742

Query: 849 -----------------------------KDADGKRKR----NLTPEALEKKISQTNAKI 890
                                         DA+ K K+      + E LEK++++ N +I
Sbjct: 743 KLAAEDPKSVLPESELEVRLKAADELKKALDAELKSKKVDPGRSSMEQLEKRLNKLNERI 802

BLAST of Bhi11G000412 vs. NCBI nr
Match: XP_038904656.1 (DNA topoisomerase 1 alpha-like isoform X1 [Benincasa hispida] >XP_038904657.1 DNA topoisomerase 1 alpha-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1729.1 bits (4477), Expect = 0.0e+00
Identity = 889/889 (100.00%), Postives = 889/889 (100.00%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRQGSDIC 60
           MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRQGSDIC
Sbjct: 1   MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRQGSDIC 60

Query: 61  STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSPIREPPVKPLGTSLKVSDSMDSRSKP 120
           STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSPIREPPVKPLGTSLKVSDSMDSRSKP
Sbjct: 61  STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSPIREPPVKPLGTSLKVSDSMDSRSKP 120

Query: 121 LVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGNSSQPNKGLVPTVPKNKVKKSSGDSD 180
           LVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGNSSQPNKGLVPTVPKNKVKKSSGDSD
Sbjct: 121 LVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGNSSQPNKGLVPTVPKNKVKKSSGDSD 180

Query: 181 DEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDKLQKPAVLSSKRPLPN 240
           DEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDKLQKPAVLSSKRPLPN
Sbjct: 181 DEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDKLQKPAVLSSKRPLPN 240

Query: 241 ADSHTPSQISTKKPKLSDSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKSS 300
           ADSHTPSQISTKKPKLSDSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKSS
Sbjct: 241 ADSHTPSQISTKKPKLSDSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKSS 300

Query: 301 SSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360
           SSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP
Sbjct: 301 SSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360

Query: 361 YQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHVI 420
           YQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHVI 420

Query: 421 QNLDDCDFTPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480
           QNLDDCDFTPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR
Sbjct: 421 QNLDDCDFTPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480

Query: 481 VEPPGLFRGRGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL 540
           VEPPGLFRGRGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL
Sbjct: 481 VEPPGLFRGRGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL 540

Query: 541 AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTR 600
           AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTR
Sbjct: 541 AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTR 600

Query: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKYE 660
           RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKYE
Sbjct: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKYE 660

Query: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720
           NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL
Sbjct: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720

Query: 721 DDMLNRGTKDGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVK 780
           DDMLNRGTKDGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVK
Sbjct: 721 DDMLNRGTKDGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVK 780

Query: 781 ELKIDLDRAKKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
           ELKIDLDRAKKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA
Sbjct: 781 ELKIDLDRAKKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840

Query: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 890
           LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 889

BLAST of Bhi11G000412 vs. NCBI nr
Match: XP_008449103.1 (PREDICTED: DNA topoisomerase 1-like [Cucumis melo] >XP_008449104.1 PREDICTED: DNA topoisomerase 1-like [Cucumis melo])

HSP 1 Score: 1618.2 bits (4189), Expect = 0.0e+00
Identity = 834/890 (93.71%), Postives = 858/890 (96.40%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRQGSDIC 60
           MAVETFGKAPMF DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQ+QRLNKL GRQGSD+C
Sbjct: 1   MAVETFGKAPMF-DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLC 60

Query: 61  STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASK-SPIREPPVKPLGTSLKVSDSMDSRSK 120
           STNGQSSNNQKRMTSLPKTSPMRSEIGSQKA K SPI+EP  K LG SLK SDSMDSR K
Sbjct: 61  STNGQSSNNQKRMTSLPKTSPMRSEIGSQKAPKSSPIQEPLTKSLGISLKASDSMDSRPK 120

Query: 121 PLVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGNSSQPNKGLVPTVPKNKVKKSSGDS 180
            LVN AVK+ENVSI  +QENSDSEDDKPLSIRLKGNS+QPNKGLV T+PKNKVKKSSGDS
Sbjct: 121 NLVNAAVKEENVSINPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTIPKNKVKKSSGDS 180

Query: 181 DDEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDKLQKPAVLSSKRPLP 240
           DDE+PLSSKFQMK  SG +GSKQ GFDEKKPLVP+VHQNGSTSRDKLQKPAVLSSKRPLP
Sbjct: 181 DDEVPLSSKFQMKHASGPMGSKQLGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP 240

Query: 241 NADSHTPSQISTKKPKLSDSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKS 300
           N DSH+PSQ STKKPKLSDSS+PLSSKLVSLKAKQEVDD+DDDIPISQR+KK  A VNKS
Sbjct: 241 NEDSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDDIPISQRLKKTPATVNKS 300

Query: 301 SSSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
           SSSK TVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP
Sbjct: 301 SSSKPTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360

Query: 361 PYQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHV 420
           PYQTHGVK+LYKGKPVDLTP+QEEVATMFAVMKDT+YMLKPKFKENFWNDWRKLLGKNHV
Sbjct: 361 PYQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420

Query: 421 IQNLDDCDFTPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF 480
           IQNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF
Sbjct: 421 IQNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF 480

Query: 481 RVEPPGLFRGRGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW 540
           RVEPPGLFRGRGEHPKMGKLKRRI PSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW
Sbjct: 481 RVEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW 540

Query: 541 LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDST 600
           LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKAR+LKDYIKNIRNAYTKDFTSKD T
Sbjct: 541 LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARVLKDYIKNIRNAYTKDFTSKDPT 600

Query: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKY 660
           RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVK VPPNLLEFNFLGKDSI+Y
Sbjct: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRY 660

Query: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASIT 720
           ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASIT
Sbjct: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASIT 720

Query: 721 LDDMLNRGTKDGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIV 780
           LDDMLNRGTKDG+V EKI TYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI+
Sbjct: 721 LDDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIL 780

Query: 781 KELKIDLDRAKKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV 840
           KELKIDLDRAKKGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV
Sbjct: 781 KELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV 840

Query: 841 ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 890
           ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 889

BLAST of Bhi11G000412 vs. NCBI nr
Match: XP_004149585.1 (DNA topoisomerase 1 alpha [Cucumis sativus] >XP_011650506.1 DNA topoisomerase 1 alpha [Cucumis sativus] >KGN56068.1 hypothetical protein Csa_010762 [Cucumis sativus])

HSP 1 Score: 1610.9 bits (4170), Expect = 0.0e+00
Identity = 830/890 (93.26%), Postives = 856/890 (96.18%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRQGSDIC 60
           MAVETFGKAPMF DDDDEDNMPIVFKR+SASRQNQLDSEIKHSQ+QRL+KL GRQGSD+C
Sbjct: 1   MAVETFGKAPMF-DDDDEDNMPIVFKRTSASRQNQLDSEIKHSQAQRLSKLPGRQGSDLC 60

Query: 61  STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASK-SPIREPPVKPLGTSLKVSDSMDSRSK 120
           STNGQSSNNQKRMTSLPKTSPMRSEIGSQKAS+ SPI EP  K LGTSLK S SMDSR K
Sbjct: 61  STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASRSSPIPEPLTKSLGTSLKASHSMDSRPK 120

Query: 121 PLVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGNSSQPNKGLVPTVPKNKVKKSSGDS 180
            LVN  VK+ENVSI  +QENSDSEDDKPLSIRLKGN +QPNKGLV TVPKNKVKKS  DS
Sbjct: 121 SLVNAVVKEENVSINPSQENSDSEDDKPLSIRLKGNPNQPNKGLVSTVPKNKVKKSLADS 180

Query: 181 DDEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDKLQKPAVLSSKRPLP 240
           DDE+PLSSKFQMK  SGVLGSKQHGFDEKKPLVP+VHQNGSTSRDKLQKPAVLSSKRPLP
Sbjct: 181 DDEVPLSSKFQMKHASGVLGSKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP 240

Query: 241 NADSHTPSQISTKKPKLSDSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKS 300
           N DSH+PSQ  TKKPKL+DSS+PL+SKLVSLKAKQEVDD+DDDIPISQRMKK+SA VNKS
Sbjct: 241 NEDSHSPSQFPTKKPKLTDSSSPLNSKLVSLKAKQEVDDDDDDIPISQRMKKVSATVNKS 300

Query: 301 SSSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
           SSSK T+SKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP
Sbjct: 301 SSSKPTISKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360

Query: 361 PYQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHV 420
           PYQTHGVK+LYKGKPVDLTP QEEVATMFAVMKDT+YMLKPKFKENFWNDWRKLLGKNHV
Sbjct: 361 PYQTHGVKMLYKGKPVDLTPPQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420

Query: 421 IQNLDDCDFTPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF 480
           IQNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKA+KEEKLKQEEKYMWAILDGVKEKVGNF
Sbjct: 421 IQNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKAVKEEKLKQEEKYMWAILDGVKEKVGNF 480

Query: 481 RVEPPGLFRGRGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW 540
           RVEPPGLFRGRGEHPKMGKLKRRI PSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW
Sbjct: 481 RVEPPGLFRGRGEHPKMGKLKRRICPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW 540

Query: 541 LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDST 600
           LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKD T
Sbjct: 541 LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDPT 600

Query: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKY 660
           RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVK VPPNLLEFNFLGKDSI+Y
Sbjct: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRY 660

Query: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASIT 720
           ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASIT
Sbjct: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASIT 720

Query: 721 LDDMLNRGTKDGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIV 780
           LDDMLNRGTK+G+V EKI TYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI+
Sbjct: 721 LDDMLNRGTKNGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIL 780

Query: 781 KELKIDLDRAKKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV 840
           KELKIDLDRAKKGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV
Sbjct: 781 KELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV 840

Query: 841 ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 890
           ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 889

BLAST of Bhi11G000412 vs. NCBI nr
Match: KAA0065816.1 (DNA topoisomerase 1-like [Cucumis melo var. makuwa] >TYJ96555.1 DNA topoisomerase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1607.0 bits (4160), Expect = 0.0e+00
Identity = 834/908 (91.85%), Postives = 858/908 (94.49%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRQGSDIC 60
           MAVETFGKAPMF DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQ+QRLNKL GRQGSD+C
Sbjct: 1   MAVETFGKAPMF-DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLC 60

Query: 61  STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASK-SPIREPPVKPLGTSLKVSDSMDSRSK 120
           STNGQSSNNQKRMTSLPKTSPMRSEIGSQKA K SPI+EP  K LG SLK SDSMDSR K
Sbjct: 61  STNGQSSNNQKRMTSLPKTSPMRSEIGSQKAPKSSPIQEPLTKSLGISLKASDSMDSRPK 120

Query: 121 PLVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGNSSQPNKGLVPTVPKNKVKKSSGDS 180
            LVN AVK+ENVSI  +QENSDSEDDKPLSIRLKGNS+QPNKGLV T+PKNKVKKSSGDS
Sbjct: 121 NLVNAAVKEENVSINPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTIPKNKVKKSSGDS 180

Query: 181 DDEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDKLQKPAVLSSKRPLP 240
           DDE+PLSSKFQMK  SG +GSKQ GFDEKKPLVP+VHQNGSTSRDKLQKPAVLSSKRPLP
Sbjct: 181 DDEVPLSSKFQMKHASGPMGSKQLGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP 240

Query: 241 NADSHTPSQISTKKPKLSDSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKS 300
           N DSH+PSQ STKKPKLSDSS+PLSSKLVSLKAKQEVDD+DDDIPISQR+KK  A VNKS
Sbjct: 241 NEDSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDDIPISQRLKKTPATVNKS 300

Query: 301 SSSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
           SSSK TVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP
Sbjct: 301 SSSKPTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360

Query: 361 PYQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHV 420
           PYQTHGVK+LYKGKPVDLTP+QEEVATMFAVMKDT+YMLKPKFKENFWNDWRKLLGKNHV
Sbjct: 361 PYQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420

Query: 421 IQNLDDCDFTPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF 480
           IQNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF
Sbjct: 421 IQNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF 480

Query: 481 RVEPPGLFRGRGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW 540
           RVEPPGLFRGRGEHPKMGKLKRRI PSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW
Sbjct: 481 RVEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW 540

Query: 541 LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDST 600
           LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKAR+LKDYIKNIRNAYTKDFTSKD T
Sbjct: 541 LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARVLKDYIKNIRNAYTKDFTSKDPT 600

Query: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKY 660
           RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVK VPPNLLEFNFLGKDSI+Y
Sbjct: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRY 660

Query: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASIT 720
           ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASIT
Sbjct: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASIT 720

Query: 721 LDDMLNRGTKDGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIV 780
           LDDMLNRGTKDG+V EKI TYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI+
Sbjct: 721 LDDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIL 780

Query: 781 KELKIDLDRAKKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV 840
           KELKIDLDRAKKGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV
Sbjct: 781 KELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV 840

Query: 841 ALGTSKINYLDPRITVAWCKRHEVPIEK------------------IFNKSLLAKFAWAM 890
           ALGTSKINYLDPRITVAWCKRHEVPIEK                  IFNKSLLAKFAWAM
Sbjct: 841 ALGTSKINYLDPRITVAWCKRHEVPIEKFKNFLLIFVIHNHNLLPQIFNKSLLAKFAWAM 900

BLAST of Bhi11G000412 vs. NCBI nr
Match: XP_023553297.1 (DNA topoisomerase 1 beta-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1577.0 bits (4082), Expect = 0.0e+00
Identity = 815/889 (91.68%), Postives = 845/889 (95.05%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRQGSDIC 60
           MAVETFGKAPMF DDDDEDNMPIVFKRSSASRQNQL+SE+KHSQSQRLN+LSGR GSD C
Sbjct: 1   MAVETFGKAPMF-DDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDAC 60

Query: 61  STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSPIREPPVKPLGTSLKVSDSMDSRSKP 120
           S NGQSSN QKRMTSLPKTSPMR EIGSQKASKS I+EP VK LGT+LKVSDSMDSR K 
Sbjct: 61  SANGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQ 120

Query: 121 LVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGNSSQPNKGLVPTVPKNKVKKSSGDSD 180
           L N AVK+EN SI  TQ+NSDSEDDKPLSIRLKGNS+ P+KG +PTV KNK+KKSS DSD
Sbjct: 121 LANAAVKEENDSIHPTQDNSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSD 180

Query: 181 DEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDKLQKPAVLSSKRPLPN 240
           DE+PLSSKFQMKS SGVLG +QHGFDEKKPL  +VHQNGSTSRDKLQKPAV+SSKRPLPN
Sbjct: 181 DEVPLSSKFQMKSASGVLGGRQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPN 240

Query: 241 ADSHTPSQISTKKPKLSDSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKSS 300
           ADSH+ SQIS+KKPKLSDSSTPLS+  VSLKAKQEV D+DD IPISQRM KMSA VNKSS
Sbjct: 241 ADSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVADDDDHIPISQRM-KMSAAVNKSS 300

Query: 301 SSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360
            SK+TVSKV SSSIKKT KKSKKPVKKSKYVKSTKLLPS+GDGQKKWTTLVHNGVIFPPP
Sbjct: 301 GSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPP 360

Query: 361 YQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHVI 420
           YQTHGVKIL+KGKPVDLTPEQEEVATMFAVMKDT+YM KPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420

Query: 421 QNLDDCDFTPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480
           QNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR
Sbjct: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480

Query: 481 VEPPGLFRGRGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL 540
           VEPPGLFRGRGEHPKMGKLK+RI PSDITINIGK APIPECPIPG+RWKEVRHDNTVTWL
Sbjct: 481 VEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWL 540

Query: 541 AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTR 600
           AFWNDPIN KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRN+YTKDFTSKD TR
Sbjct: 541 AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTR 600

Query: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKYE 660
           RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVK VPPNLLEFNFLGKDSIKYE
Sbjct: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE 660

Query: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720
           NTVEVELPVFKAITQFQSGKSG+DDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL
Sbjct: 661 NTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720

Query: 721 DDMLNRGTKDGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVK 780
           DDMLNRGTKDG+V EKI TYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVK
Sbjct: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVK 780

Query: 781 ELKIDLDRAKKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
           ELKIDLDRAKKGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA
Sbjct: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840

Query: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 890
           LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887

BLAST of Bhi11G000412 vs. ExPASy TrEMBL
Match: A0A1S3BM72 (DNA topoisomerase I OS=Cucumis melo OX=3656 GN=LOC103491068 PE=3 SV=1)

HSP 1 Score: 1618.2 bits (4189), Expect = 0.0e+00
Identity = 834/890 (93.71%), Postives = 858/890 (96.40%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRQGSDIC 60
           MAVETFGKAPMF DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQ+QRLNKL GRQGSD+C
Sbjct: 1   MAVETFGKAPMF-DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLC 60

Query: 61  STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASK-SPIREPPVKPLGTSLKVSDSMDSRSK 120
           STNGQSSNNQKRMTSLPKTSPMRSEIGSQKA K SPI+EP  K LG SLK SDSMDSR K
Sbjct: 61  STNGQSSNNQKRMTSLPKTSPMRSEIGSQKAPKSSPIQEPLTKSLGISLKASDSMDSRPK 120

Query: 121 PLVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGNSSQPNKGLVPTVPKNKVKKSSGDS 180
            LVN AVK+ENVSI  +QENSDSEDDKPLSIRLKGNS+QPNKGLV T+PKNKVKKSSGDS
Sbjct: 121 NLVNAAVKEENVSINPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTIPKNKVKKSSGDS 180

Query: 181 DDEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDKLQKPAVLSSKRPLP 240
           DDE+PLSSKFQMK  SG +GSKQ GFDEKKPLVP+VHQNGSTSRDKLQKPAVLSSKRPLP
Sbjct: 181 DDEVPLSSKFQMKHASGPMGSKQLGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP 240

Query: 241 NADSHTPSQISTKKPKLSDSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKS 300
           N DSH+PSQ STKKPKLSDSS+PLSSKLVSLKAKQEVDD+DDDIPISQR+KK  A VNKS
Sbjct: 241 NEDSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDDIPISQRLKKTPATVNKS 300

Query: 301 SSSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
           SSSK TVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP
Sbjct: 301 SSSKPTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360

Query: 361 PYQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHV 420
           PYQTHGVK+LYKGKPVDLTP+QEEVATMFAVMKDT+YMLKPKFKENFWNDWRKLLGKNHV
Sbjct: 361 PYQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420

Query: 421 IQNLDDCDFTPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF 480
           IQNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF
Sbjct: 421 IQNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF 480

Query: 481 RVEPPGLFRGRGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW 540
           RVEPPGLFRGRGEHPKMGKLKRRI PSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW
Sbjct: 481 RVEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW 540

Query: 541 LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDST 600
           LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKAR+LKDYIKNIRNAYTKDFTSKD T
Sbjct: 541 LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARVLKDYIKNIRNAYTKDFTSKDPT 600

Query: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKY 660
           RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVK VPPNLLEFNFLGKDSI+Y
Sbjct: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRY 660

Query: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASIT 720
           ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASIT
Sbjct: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASIT 720

Query: 721 LDDMLNRGTKDGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIV 780
           LDDMLNRGTKDG+V EKI TYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI+
Sbjct: 721 LDDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIL 780

Query: 781 KELKIDLDRAKKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV 840
           KELKIDLDRAKKGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV
Sbjct: 781 KELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV 840

Query: 841 ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 890
           ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 889

BLAST of Bhi11G000412 vs. ExPASy TrEMBL
Match: A0A0A0L2W2 (DNA topoisomerase I OS=Cucumis sativus OX=3659 GN=Csa_3G063680 PE=3 SV=1)

HSP 1 Score: 1610.9 bits (4170), Expect = 0.0e+00
Identity = 830/890 (93.26%), Postives = 856/890 (96.18%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRQGSDIC 60
           MAVETFGKAPMF DDDDEDNMPIVFKR+SASRQNQLDSEIKHSQ+QRL+KL GRQGSD+C
Sbjct: 1   MAVETFGKAPMF-DDDDEDNMPIVFKRTSASRQNQLDSEIKHSQAQRLSKLPGRQGSDLC 60

Query: 61  STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASK-SPIREPPVKPLGTSLKVSDSMDSRSK 120
           STNGQSSNNQKRMTSLPKTSPMRSEIGSQKAS+ SPI EP  K LGTSLK S SMDSR K
Sbjct: 61  STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASRSSPIPEPLTKSLGTSLKASHSMDSRPK 120

Query: 121 PLVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGNSSQPNKGLVPTVPKNKVKKSSGDS 180
            LVN  VK+ENVSI  +QENSDSEDDKPLSIRLKGN +QPNKGLV TVPKNKVKKS  DS
Sbjct: 121 SLVNAVVKEENVSINPSQENSDSEDDKPLSIRLKGNPNQPNKGLVSTVPKNKVKKSLADS 180

Query: 181 DDEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDKLQKPAVLSSKRPLP 240
           DDE+PLSSKFQMK  SGVLGSKQHGFDEKKPLVP+VHQNGSTSRDKLQKPAVLSSKRPLP
Sbjct: 181 DDEVPLSSKFQMKHASGVLGSKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP 240

Query: 241 NADSHTPSQISTKKPKLSDSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKS 300
           N DSH+PSQ  TKKPKL+DSS+PL+SKLVSLKAKQEVDD+DDDIPISQRMKK+SA VNKS
Sbjct: 241 NEDSHSPSQFPTKKPKLTDSSSPLNSKLVSLKAKQEVDDDDDDIPISQRMKKVSATVNKS 300

Query: 301 SSSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
           SSSK T+SKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP
Sbjct: 301 SSSKPTISKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360

Query: 361 PYQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHV 420
           PYQTHGVK+LYKGKPVDLTP QEEVATMFAVMKDT+YMLKPKFKENFWNDWRKLLGKNHV
Sbjct: 361 PYQTHGVKMLYKGKPVDLTPPQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420

Query: 421 IQNLDDCDFTPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF 480
           IQNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKA+KEEKLKQEEKYMWAILDGVKEKVGNF
Sbjct: 421 IQNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKAVKEEKLKQEEKYMWAILDGVKEKVGNF 480

Query: 481 RVEPPGLFRGRGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW 540
           RVEPPGLFRGRGEHPKMGKLKRRI PSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW
Sbjct: 481 RVEPPGLFRGRGEHPKMGKLKRRICPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW 540

Query: 541 LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDST 600
           LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKD T
Sbjct: 541 LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDPT 600

Query: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKY 660
           RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVK VPPNLLEFNFLGKDSI+Y
Sbjct: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRY 660

Query: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASIT 720
           ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASIT
Sbjct: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASIT 720

Query: 721 LDDMLNRGTKDGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIV 780
           LDDMLNRGTK+G+V EKI TYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI+
Sbjct: 721 LDDMLNRGTKNGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIL 780

Query: 781 KELKIDLDRAKKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV 840
           KELKIDLDRAKKGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV
Sbjct: 781 KELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV 840

Query: 841 ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 890
           ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 889

BLAST of Bhi11G000412 vs. ExPASy TrEMBL
Match: A0A5A7VHI9 (DNA topoisomerase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G002170 PE=3 SV=1)

HSP 1 Score: 1607.0 bits (4160), Expect = 0.0e+00
Identity = 834/908 (91.85%), Postives = 858/908 (94.49%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRQGSDIC 60
           MAVETFGKAPMF DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQ+QRLNKL GRQGSD+C
Sbjct: 1   MAVETFGKAPMF-DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLC 60

Query: 61  STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASK-SPIREPPVKPLGTSLKVSDSMDSRSK 120
           STNGQSSNNQKRMTSLPKTSPMRSEIGSQKA K SPI+EP  K LG SLK SDSMDSR K
Sbjct: 61  STNGQSSNNQKRMTSLPKTSPMRSEIGSQKAPKSSPIQEPLTKSLGISLKASDSMDSRPK 120

Query: 121 PLVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGNSSQPNKGLVPTVPKNKVKKSSGDS 180
            LVN AVK+ENVSI  +QENSDSEDDKPLSIRLKGNS+QPNKGLV T+PKNKVKKSSGDS
Sbjct: 121 NLVNAAVKEENVSINPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTIPKNKVKKSSGDS 180

Query: 181 DDEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDKLQKPAVLSSKRPLP 240
           DDE+PLSSKFQMK  SG +GSKQ GFDEKKPLVP+VHQNGSTSRDKLQKPAVLSSKRPLP
Sbjct: 181 DDEVPLSSKFQMKHASGPMGSKQLGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP 240

Query: 241 NADSHTPSQISTKKPKLSDSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKS 300
           N DSH+PSQ STKKPKLSDSS+PLSSKLVSLKAKQEVDD+DDDIPISQR+KK  A VNKS
Sbjct: 241 NEDSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDDIPISQRLKKTPATVNKS 300

Query: 301 SSSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
           SSSK TVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP
Sbjct: 301 SSSKPTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360

Query: 361 PYQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHV 420
           PYQTHGVK+LYKGKPVDLTP+QEEVATMFAVMKDT+YMLKPKFKENFWNDWRKLLGKNHV
Sbjct: 361 PYQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420

Query: 421 IQNLDDCDFTPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF 480
           IQNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF
Sbjct: 421 IQNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF 480

Query: 481 RVEPPGLFRGRGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW 540
           RVEPPGLFRGRGEHPKMGKLKRRI PSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW
Sbjct: 481 RVEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW 540

Query: 541 LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDST 600
           LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKAR+LKDYIKNIRNAYTKDFTSKD T
Sbjct: 541 LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARVLKDYIKNIRNAYTKDFTSKDPT 600

Query: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKY 660
           RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVK VPPNLLEFNFLGKDSI+Y
Sbjct: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRY 660

Query: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASIT 720
           ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASIT
Sbjct: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASIT 720

Query: 721 LDDMLNRGTKDGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIV 780
           LDDMLNRGTKDG+V EKI TYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI+
Sbjct: 721 LDDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIL 780

Query: 781 KELKIDLDRAKKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV 840
           KELKIDLDRAKKGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV
Sbjct: 781 KELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV 840

Query: 841 ALGTSKINYLDPRITVAWCKRHEVPIEK------------------IFNKSLLAKFAWAM 890
           ALGTSKINYLDPRITVAWCKRHEVPIEK                  IFNKSLLAKFAWAM
Sbjct: 841 ALGTSKINYLDPRITVAWCKRHEVPIEKFKNFLLIFVIHNHNLLPQIFNKSLLAKFAWAM 900

BLAST of Bhi11G000412 vs. ExPASy TrEMBL
Match: A0A6J1KZE0 (DNA topoisomerase I OS=Cucurbita maxima OX=3661 GN=LOC111500229 PE=3 SV=1)

HSP 1 Score: 1572.0 bits (4069), Expect = 0.0e+00
Identity = 814/889 (91.56%), Postives = 844/889 (94.94%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRQGSDIC 60
           MAVETFGKA MF DDDDEDNMPIVFKRSSASRQNQL+SE+KHSQSQRLN+LSGR GSD C
Sbjct: 1   MAVETFGKATMF-DDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDAC 60

Query: 61  STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSPIREPPVKPLGTSLKVSDSMDSRSKP 120
           STNGQSSN QKRMTSLPKTSPMR EIGSQKASKS I+EP VK LGT+LKVSDSMDSR K 
Sbjct: 61  STNGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQ 120

Query: 121 LVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGNSSQPNKGLVPTVPKNKVKKSSGDSD 180
           LVN AVK+EN SI   QENSDSEDDKPLSIRLKGNS+ P+KG +PTV KNK+KKSS DSD
Sbjct: 121 LVNAAVKEENDSIHPAQENSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSD 180

Query: 181 DEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDKLQKPAVLSSKRPLPN 240
           DE+PLSSKF+MKS SGVLG KQHGFDEKKPL  +VHQNGSTS DKLQKPAV+SSKRPLPN
Sbjct: 181 DEVPLSSKFRMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSSDKLQKPAVVSSKRPLPN 240

Query: 241 ADSHTPSQISTKKPKLSDSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKSS 300
           ADSH+ SQIS+KKPKLSDSSTPLS+  VSLKAKQEV D+DD IPISQRM KMSA VNKSS
Sbjct: 241 ADSHSSSQISSKKPKLSDSSTPLSTHTVSLKAKQEVVDDDDHIPISQRM-KMSAAVNKSS 300

Query: 301 SSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360
            SK+TVSKV SSSIKKT KKSKKPVKKSKYVKSTKLLPS+GDGQKKWTTLVHNGVIFPPP
Sbjct: 301 GSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPP 360

Query: 361 YQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHVI 420
           YQTHGVKIL+KGKPVDLTPEQEEVATMFAVMKDT+YM KPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420

Query: 421 QNLDDCDFTPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480
           QNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR
Sbjct: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480

Query: 481 VEPPGLFRGRGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL 540
           VEPPGLFRGRGEHPKMGKLK+RI PSDITINIGK APIPECPIPG+RWKEVRHDNTVTWL
Sbjct: 481 VEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWL 540

Query: 541 AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTR 600
           AFWNDPIN KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRN+YTKDFTSKD TR
Sbjct: 541 AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTR 600

Query: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKYE 660
           RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVK VPPNLLEFNFLGKDSIKYE
Sbjct: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE 660

Query: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720
           NTVEVELPVFKAITQFQSGKSG+DDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL
Sbjct: 661 NTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720

Query: 721 DDMLNRGTKDGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVK 780
           DDMLNRGTKDG+V EKI TYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI+K
Sbjct: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780

Query: 781 ELKIDLDRAKKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
           ELKIDLDRAKKGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA
Sbjct: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840

Query: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 890
           LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887

BLAST of Bhi11G000412 vs. ExPASy TrEMBL
Match: A0A6J1EWI0 (DNA topoisomerase I OS=Cucurbita moschata OX=3662 GN=LOC111438616 PE=3 SV=1)

HSP 1 Score: 1570.8 bits (4066), Expect = 0.0e+00
Identity = 813/889 (91.45%), Postives = 843/889 (94.83%), Query Frame = 0

Query: 1   MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRQGSDIC 60
           MAVETFGKAPMF DDDDEDNMPIVFKRSSASRQNQL+SE+KHSQSQRLN+LSGR GSD C
Sbjct: 1   MAVETFGKAPMF-DDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDAC 60

Query: 61  STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSPIREPPVKPLGTSLKVSDSMDSRSKP 120
           S NGQSSN QKRMTSLPKTSPMR EIGSQKA+KS I+EP VK LGT+LKVSDSMDSR K 
Sbjct: 61  SANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQ 120

Query: 121 LVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGNSSQPNKGLVPTVPKNKVKKSSGDSD 180
           L N AVK+EN SI  TQENSDSEDDKPLSIRLKGNS+ P+KG +PTV KNK+KKSS DSD
Sbjct: 121 LANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSD 180

Query: 181 DEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDKLQKPAVLSSKRPLPN 240
           DE+PLSSKFQMKS SGVLG KQHGF EKKPL  +VHQNGSTSRDKLQKPAV+SSKRPLPN
Sbjct: 181 DEVPLSSKFQMKSASGVLGGKQHGFHEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPN 240

Query: 241 ADSHTPSQISTKKPKLSDSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKSS 300
           ADSH+ SQIS+KKPKLSDSSTPLS+  VSLKAKQEV D+DD IPISQRM KMSA VNKSS
Sbjct: 241 ADSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRM-KMSAAVNKSS 300

Query: 301 SSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360
            SK+TVSKV SSSIKKT KKSKKPVKKSKYVKSTKLLPS+GDGQKKWTTLVHNGVIFPPP
Sbjct: 301 GSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPP 360

Query: 361 YQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHVI 420
           YQTHGVKIL+KGKPVDLT EQEEVATMFAVMKDT+YM KPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKILHKGKPVDLTTEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420

Query: 421 QNLDDCDFTPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480
           QNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR
Sbjct: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480

Query: 481 VEPPGLFRGRGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL 540
           VEPPGLFRGRGEHPKMGKLK+RI PSDITINIGK APIPECPIPG+RWKEVRHDNTVTWL
Sbjct: 481 VEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWL 540

Query: 541 AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTR 600
           AFWNDPIN KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRN+YTKDFTSKD TR
Sbjct: 541 AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTR 600

Query: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKYE 660
           RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVK VPPNLLEFNFLGKDSIKYE
Sbjct: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE 660

Query: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720
           NTVEVELPVFKAITQFQSGKSG+DDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL
Sbjct: 661 NTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720

Query: 721 DDMLNRGTKDGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVK 780
           DDMLNRGTKDG+V EKI TYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI+K
Sbjct: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780

Query: 781 ELKIDLDRAKKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
           ELKIDLDRAKKGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA
Sbjct: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840

Query: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 890
           LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G55300.18.0e-29961.28DNA topoisomerase I alpha [more]
AT5G55300.22.6e-29761.26DNA topoisomerase I alpha [more]
AT5G55310.14.5e-29460.89DNA topoisomerase 1 beta [more]
AT5G55300.34.8e-11876.38DNA topoisomerase I alpha [more]
AT4G26701.11.9e-2988.24DNA binding;DNA topoisomerase type Is [more]
Match NameE-valueIdentityDescription
P301811.1e-29761.28DNA topoisomerase 1 alpha OS=Arabidopsis thaliana OX=3702 GN=TOP1A PE=1 SV=1[more]
Q9FJ796.4e-29360.89DNA topoisomerase 1 beta OS=Arabidopsis thaliana OX=3702 GN=TOP1B PE=1 SV=1[more]
P931191.3e-24260.91DNA topoisomerase 1 OS=Daucus carota OX=4039 GN=TOP1 PE=2 SV=1[more]
Q54RC33.3e-14840.25DNA topoisomerase 1 OS=Dictyostelium discoideum OX=44689 GN=top1 PE=3 SV=1[more]
P077993.6e-14740.31DNA topoisomerase 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=28... [more]
Match NameE-valueIdentityDescription
XP_038904656.10.0e+00100.00DNA topoisomerase 1 alpha-like isoform X1 [Benincasa hispida] >XP_038904657.1 DN... [more]
XP_008449103.10.0e+0093.71PREDICTED: DNA topoisomerase 1-like [Cucumis melo] >XP_008449104.1 PREDICTED: DN... [more]
XP_004149585.10.0e+0093.26DNA topoisomerase 1 alpha [Cucumis sativus] >XP_011650506.1 DNA topoisomerase 1 ... [more]
KAA0065816.10.0e+0091.85DNA topoisomerase 1-like [Cucumis melo var. makuwa] >TYJ96555.1 DNA topoisomeras... [more]
XP_023553297.10.0e+0091.68DNA topoisomerase 1 beta-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A1S3BM720.0e+0093.71DNA topoisomerase I OS=Cucumis melo OX=3656 GN=LOC103491068 PE=3 SV=1[more]
A0A0A0L2W20.0e+0093.26DNA topoisomerase I OS=Cucumis sativus OX=3659 GN=Csa_3G063680 PE=3 SV=1[more]
A0A5A7VHI90.0e+0091.85DNA topoisomerase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G... [more]
A0A6J1KZE00.0e+0091.56DNA topoisomerase I OS=Cucurbita maxima OX=3661 GN=LOC111500229 PE=3 SV=1[more]
A0A6J1EWI00.0e+0091.45DNA topoisomerase I OS=Cucurbita moschata OX=3662 GN=LOC111438616 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 765..792
NoneNo IPR availableCOILSCoilCoilcoord: 435..463
NoneNo IPR availableCOILSCoilCoilcoord: 816..836
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..285
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 26..91
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 182..197
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 271..285
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 109..138
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 238..268
NoneNo IPR availablePANTHERPTHR10290:SF17DNA TOPOISOMERASE Icoord: 136..889
NoneNo IPR availablePANTHERPTHR10290DNA TOPOISOMERASE Icoord: 136..889
IPR001631DNA topoisomerase IPRINTSPR00416EUTPISMRASEIcoord: 842..853
score: 72.62
coord: 647..663
score: 57.14
coord: 705..719
score: 80.0
coord: 602..621
score: 67.14
coord: 486..495
score: 71.07
coord: 625..639
score: 66.19
IPR013499DNA topoisomerase I, eukaryotic-typeSMARTSM00435topeucoord: 486..862
e-value: 1.9E-264
score: 894.5
IPR025834Topoisomerase I C-terminal domainPFAMPF14370Topo_C_assoccoord: 821..889
e-value: 1.6E-35
score: 120.8
IPR014711DNA topoisomerase I, catalytic core, alpha-helical subdomain, eukaryotic-typeGENE3D3.90.15.10Topoisomerase I; Chain A, domain 3coord: 560..708
e-value: 3.5E-57
score: 194.8
IPR013030DNA topoisomerase I, DNA binding, N-terminal domain 2GENE3D2.170.11.10DNA Topoisomerase I, domain 2coord: 342..557
e-value: 2.2E-95
score: 320.4
IPR008336DNA topoisomerase I, DNA binding, eukaryotic-typePFAMPF02919Topoisom_I_Ncoord: 346..557
e-value: 1.6E-93
score: 312.1
IPR013034DNA topoisomerase I, DNA binding, N-terminal domain 1GENE3D1.10.10.41coord: 362..445
e-value: 2.2E-95
score: 320.4
IPR014727DNA topoisomerase I, catalytic core, alpha/beta subdomainGENE3D1.10.132.10coord: 709..889
e-value: 1.2E-67
score: 229.1
IPR013500DNA topoisomerase I, catalytic core, eukaryotic-typePFAMPF01028Topoisom_Icoord: 560..783
e-value: 1.6E-91
score: 305.7
IPR013500DNA topoisomerase I, catalytic core, eukaryotic-typeCDDcd00659Topo_IB_Ccoord: 566..759
e-value: 1.32082E-78
score: 251.813
IPR018521DNA topoisomerase I, active sitePROSITEPS00176TOPOISOMERASE_I_EUKcoord: 835..853
IPR036202DNA topoisomerase I, DNA binding, eukaryotic-type, N-terminal domain superfamilySUPERFAMILY56741Eukaryotic DNA topoisomerase I, N-terminal DNA-binding fragmentcoord: 341..558
IPR011010DNA breaking-rejoining enzyme, catalytic coreSUPERFAMILY56349DNA breaking-rejoining enzymescoord: 560..886

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi11M000412Bhi11M000412mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006338 chromatin remodeling
biological_process GO:0007059 chromosome segregation
biological_process GO:0006260 DNA replication
biological_process GO:0006265 DNA topological change
cellular_component GO:0005694 chromosome
cellular_component GO:0005730 nucleolus
molecular_function GO:0003677 DNA binding
molecular_function GO:0003917 DNA topoisomerase type I (single strand cut, ATP-independent) activity