Homology
BLAST of Bhi11G000412 vs. TAIR 10
Match:
AT5G55300.1 (DNA topoisomerase I alpha )
HSP 1 Score: 1023.8 bits (2646), Expect = 8.0e-299
Identity = 576/940 (61.28%), Postives = 689/940 (73.30%), Query Frame = 0
Query: 1 MAVETFGKAPMFDD--DDDEDNMPIVFKRSS--ASRQNQLDSEIKHSQSQRLNKLSGRQG 60
M ET K P+ D+ D +D+ P+ FKR++ AS NQ S + S++ K+S +
Sbjct: 1 MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPMR- 60
Query: 61 SDICSTNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSPIREPPVKPLGTSLKVSDSMDS 120
S + S NG + +N KTS ++S + S + SP + P + +++K
Sbjct: 61 SPVTSPNGTTPSN--------KTSIVKSSMPSSSSKASPAKSPLRNDMPSTVK------D 120
Query: 121 RSKPLVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGN----------SSQPNKGLVPT 180
RS+ ++K+ + E+ DSEDD+PLS L GN S QP K
Sbjct: 121 RSQ------LQKDQSECKI--EHEDSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGD 180
Query: 181 VPKNKVKKSSGD-SDDEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDK 240
P ++ + D SDDE P+SS F+ K SG+ G Q DEKKPLV ++HQNGST +++
Sbjct: 181 RPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVKNE 240
Query: 241 LQKPAVLSSKRPLPNADSHTPSQISTKKPKLSDSSTPLSSK--------------LVS-- 300
+ VL KRPL S Q S KK K+S S T + K LVS
Sbjct: 241 VPNGKVL-GKRPLEKNSS--ADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVSPK 300
Query: 301 LKAKQ-------EVDDEDDDIPISQRMKKMSAPVNKSSSSKQTVS-------------KV 360
+KAKQ DD+DDD+PIS+R K S+ N SS+ + V V
Sbjct: 301 MKAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKARNV 360
Query: 361 VSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQTHGVKIL 420
VS + K +KK K SKY S+K PS+GDGQKKWTTLVHNGVIFPPPY+ HG+KIL
Sbjct: 361 VSPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGIKIL 420
Query: 421 YKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHVIQNLDDCDFT 480
YKGKPVDLT EQEEVATMFAVM++T+Y KP+F+ENFWNDWR+LLGK HVIQ LDDCDFT
Sbjct: 421 YKGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDCDFT 480
Query: 481 PIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPGLFRG 540
PIY+W+ +EKEKKKQM+TEEKKALKEEK+KQEEKYMWA++DGVKEK+GNFRVEPPGLFRG
Sbjct: 481 PIYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPPGLFRG 540
Query: 541 RGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINQ 600
RGEHPKMGKLK+RIHP +IT+NIGKGAPIPECPI GERWKEV+HDNTVTWLAFW DPIN
Sbjct: 541 RGEHPKMGKLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADPINP 600
Query: 601 KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTRRQIAVATYL 660
KEFKYVFL A SSLKG SDKEKYEKAR L D+I NIR YTK+FT+KD RQIAVATYL
Sbjct: 601 KEFKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVATYL 660
Query: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKYENTVEVELPV 720
IDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE V
Sbjct: 661 IDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLV 720
Query: 721 FKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780
+KAI QFQ+GKS TDDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TK
Sbjct: 721 YKAIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETK 780
Query: 781 DGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKELKIDLDRA 840
DGDV +KI YQ ANKEVAIICNHQRTVSK+HGAQ+ +L +I EL++++KELK +LDRA
Sbjct: 781 DGDVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRA 840
Query: 841 KKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL 890
KKGKPPL+ +DGK+ R+L P A EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYL
Sbjct: 841 KKGKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYL 900
BLAST of Bhi11G000412 vs. TAIR 10
Match:
AT5G55300.2 (DNA topoisomerase I alpha )
HSP 1 Score: 1018.8 bits (2633), Expect = 2.6e-297
Identity = 574/937 (61.26%), Postives = 686/937 (73.21%), Query Frame = 0
Query: 1 MAVETFGKAPMFDD--DDDEDNMPIVFKRSS--ASRQNQLDSEIKHSQSQRLNKLSGRQG 60
M ET K P+ D+ D +D+ P+ FKR++ AS NQ S + S++ K+S +
Sbjct: 1 MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPMR- 60
Query: 61 SDICSTNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSPIREPPVKPLGTSLKVSDSMDS 120
S + S NG + +N KTS ++S + S + SP + P + +++K
Sbjct: 61 SPVTSPNGTTPSN--------KTSIVKSSMPSSSSKASPAKSPLRNDMPSTVK------D 120
Query: 121 RSKPLVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGN----------SSQPNKGLVPT 180
RS+ ++K+ + E+ DSEDD+PLS L GN S QP K
Sbjct: 121 RSQ------LQKDQSECKI--EHEDSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGD 180
Query: 181 VPKNKVKKSSGD-SDDEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDK 240
P ++ + D SDDE P+SS F+ K SG+ G Q DEKKPLV ++HQNGST +++
Sbjct: 181 RPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVKNE 240
Query: 241 LQKPAVLSSKRPLPNADSHTPSQISTKKPKLSDSSTPLSSK--------------LVS-- 300
+ VL KRPL S Q S KK K+S S T + K LVS
Sbjct: 241 VPNGKVL-GKRPLEKNSS--ADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVSPK 300
Query: 301 LKAKQ-------EVDDEDDDIPISQRMKKMSAPVNKSSSSKQTVS-------------KV 360
+KAKQ DD+DDD+PIS+R K S+ N SS+ + V V
Sbjct: 301 MKAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKARNV 360
Query: 361 VSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQTHGVKIL 420
VS + K +KK K SKY S+K PS+GDGQKKWTTLVHNGVIFPPPY+ HG+KIL
Sbjct: 361 VSPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGIKIL 420
Query: 421 YKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHVIQNLDDCDFT 480
YKGKPVDLT EQEEVATMFAVM++T+Y KP+F+ENFWNDWR+LLGK HVIQ LDDCDFT
Sbjct: 421 YKGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDCDFT 480
Query: 481 PIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPGLFRG 540
PIY+W+ +EKEKKKQM+TEEKKALKEEK+KQEEKYMWA++DGVKEK+GNFRVEPPGLFRG
Sbjct: 481 PIYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPPGLFRG 540
Query: 541 RGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINQ 600
RGEHPKMGKLK+RIHP +IT+NIGKGAPIPECPI GERWKEV+HDNTVTWLAFW DPIN
Sbjct: 541 RGEHPKMGKLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADPINP 600
Query: 601 KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTRRQIAVATYL 660
KEFKYVFL A SSLKG SDKEKYEKAR L D+I NIR YTK+FT+KD RQIAVATYL
Sbjct: 601 KEFKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVATYL 660
Query: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKYENTVEVELPV 720
IDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE V
Sbjct: 661 IDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLV 720
Query: 721 FKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780
+KAI QFQ+GKS TDDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TK
Sbjct: 721 YKAIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETK 780
Query: 781 DGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKELKIDLDRA 840
DGDV +KI YQ ANKEVAIICNHQRTVSK+HGAQ+ +L +I EL++++KELK +LDRA
Sbjct: 781 DGDVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRA 840
Query: 841 KKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL 887
KKGKPPL+ +DGK+ R+L P A EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYL
Sbjct: 841 KKGKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYL 900
BLAST of Bhi11G000412 vs. TAIR 10
Match:
AT5G55310.1 (DNA topoisomerase 1 beta )
HSP 1 Score: 1008.1 bits (2605), Expect = 4.5e-294
Identity = 573/941 (60.89%), Postives = 676/941 (71.84%), Query Frame = 0
Query: 1 MAVETFGKAPMFD-----DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRQ 60
MA E F K + + +D+DED++P+VFKR+S ++ ++ +N + +
Sbjct: 1 MATEAFVKPVVPNGHDGYEDEDEDDIPLVFKRNS-------NTAATTNRPSPIN--NAMR 60
Query: 61 GSDICSTNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSPIREPPVKPLGTSLKVSDSMD 120
S I ST P TSP RS S ++S P + S S D
Sbjct: 61 NSAIGSTKSSPPMRS------PLTSPNRSASSSTRSSMMKPALPSSSSVQRSTLKSPLRD 120
Query: 121 SRSKPLVNTAVKKEN----VSIILTQENSDSEDDKPLSIRLKGNS-------SQPNKGLV 180
RS K+ N + ++ DSEDDKPLS RLK +S S +G
Sbjct: 121 DRS-----VVAKERNGFGKAPSVSKSDDEDSEDDKPLSARLKLDSKEVTKQPSSSGRGST 180
Query: 181 PTVPKNKVKKSSGDS--------DDEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVH 240
+ + G S D+ P+SS Q K+ G SK ++K+PLV +
Sbjct: 181 QQAVQKSNMRPQGLSDYTKKKVLDERAPMSSTVQTKTSVGTSSSKPVHIEQKRPLVNNID 240
Query: 241 QNG---STSRDKLQKPAVLSSKRPLPNADSHTPSQISTKKPKLS---------------- 300
+NG T Q PA KRPL S +Q S K+PKLS
Sbjct: 241 RNGLKPKTEGHSSQAPA----KRPLEKGSS--SNQSSVKRPKLSEPARPVKVEQGSHISA 300
Query: 301 ---------DSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKSSSSKQTVSK 360
D+S PL + ++ K++ D DD +PI+ RMK S+ NKSSS+K + SK
Sbjct: 301 TQDAKGKNLDASKPLRANQATV--KEDNSDGDDHVPIASRMKSDSSN-NKSSSAKPSSSK 360
Query: 361 VVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQTHGVKI 420
+++SS + +K K VK SKY KS+K LPS GDGQKKW TL HNGVIFPPPY+ HGVKI
Sbjct: 361 MIASSSRTIAQKPNKWVKDSKYSKSSKSLPS-GDGQKKWKTLQHNGVIFPPPYKRHGVKI 420
Query: 421 LYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHVIQNLDDCDF 480
L++GKPVDLTPEQEEVATMFAVM++TEY KPKF+ENFWNDWRKLLGKNH+I++LDDCDF
Sbjct: 421 LFQGKPVDLTPEQEEVATMFAVMRETEYYKKPKFRENFWNDWRKLLGKNHMIKSLDDCDF 480
Query: 481 TPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPGLFR 540
TPIY+WY +EKE KKQMT EEK+ +KEEKLKQEEKYMWA+LDGV+E++GNFRVEPPGLFR
Sbjct: 481 TPIYEWYMQEKETKKQMTAEEKRIVKEEKLKQEEKYMWAVLDGVRERIGNFRVEPPGLFR 540
Query: 541 GRGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFWNDPIN 600
GRGEHPKMGKLK+RI P DITINIGK APIPECPIPGERWKEV+HDNTVTWLAFW+DPIN
Sbjct: 541 GRGEHPKMGKLKKRIRPCDITINIGKEAPIPECPIPGERWKEVKHDNTVTWLAFWSDPIN 600
Query: 601 QKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTRRQIAVATY 660
KEFKYVFLAASSSLKGQSDKEKYEKAR L ++I +IR AYTKDF +KD T+RQIAVATY
Sbjct: 601 PKEFKYVFLAASSSLKGQSDKEKYEKARKLHNHIGSIRAAYTKDFNNKDVTKRQIAVATY 660
Query: 661 LIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKYENTVEVELP 720
LIDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN L+F+FLGKDSI+Y NTVEVE
Sbjct: 661 LIDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKLKFDFLGKDSIQYVNTVEVEPL 720
Query: 721 VFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGT 780
V+KAI QFQ+GKS TDDLFD+LDTSKLN HLK+LM GLTAKVFRTYNASITLD ML++ T
Sbjct: 721 VYKAIGQFQAGKSKTDDLFDELDTSKLNTHLKELMAGLTAKVFRTYNASITLDLMLSKET 780
Query: 781 KDGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKELKIDLDR 840
+DGDV EK+ YQ ANKEVAIICNHQRTVSKSHGAQ+ +L KI ELR+ +KEL IDLDR
Sbjct: 781 RDGDVPEKVVVYQQANKEVAIICNHQRTVSKSHGAQVEKLAVKIEELREQIKELNIDLDR 840
Query: 841 AKKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINY 890
AKKG+ PL +DGKRKRNLTPEALEKKI QT KIEK+ERDM+TKED+KTVALGTSKINY
Sbjct: 841 AKKGRTPLMGSDGKRKRNLTPEALEKKIMQTQGKIEKMERDMQTKEDMKTVALGTSKINY 900
BLAST of Bhi11G000412 vs. TAIR 10
Match:
AT5G55300.3 (DNA topoisomerase I alpha )
HSP 1 Score: 423.3 bits (1087), Expect = 4.8e-118
Identity = 207/271 (76.38%), Postives = 243/271 (89.67%), Query Frame = 0
Query: 619 NEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKYENTVEVELPVFKAITQFQS 678
++ DDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE V+KAI QFQ+
Sbjct: 291 DDDDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLVYKAIGQFQA 350
Query: 679 GKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTKDGDVVEKIA 738
GKS TDDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TKDGDV +KI
Sbjct: 351 GKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETKDGDVTQKIV 410
Query: 739 TYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKELKIDLDRAKKGKPPLKD 798
YQ ANKEVAIICNHQRTVSK+HGAQ+ +L +I EL++++KELK +LDRAKKGKPPL+
Sbjct: 411 VYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRAKKGKPPLEG 470
Query: 799 ADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAWC 858
+DGK+ R+L P A EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYLDPRITVAWC
Sbjct: 471 SDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYLDPRITVAWC 530
Query: 859 KRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 890
KRHEVPIEKIF KSLL KFAWAMDV+P++RF
Sbjct: 531 KRHEVPIEKIFTKSLLEKFAWAMDVEPEYRF 561
HSP 2 Score: 93.6 bits (231), Expect = 8.6e-19
Identity = 104/305 (34.10%), Postives = 157/305 (51.48%), Query Frame = 0
Query: 1 MAVETFGKAPMFDD--DDDEDNMPIVFKRSS--ASRQNQLDSEIKHSQSQRLNKLSGRQG 60
M ET K P+ D+ D +D+ P+ FKR++ AS NQ S + S++ K+S +
Sbjct: 1 MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPMR- 60
Query: 61 SDICSTNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSPIREPPVKPLGTSLKVSDSMDS 120
S + S NG + +N KTS ++S + S + SP + P + +++K
Sbjct: 61 SPVTSPNGTTPSN--------KTSIVKSSMPSSSSKASPAKSPLRNDMPSTVK------D 120
Query: 121 RSKPLVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGN----------SSQPNKGLVPT 180
RS+ ++K+ + E+ DSEDD+PLS L GN S QP K
Sbjct: 121 RSQ------LQKDQSECKI--EHEDSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGD 180
Query: 181 VPKNKVKKSSGD-SDDEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDK 240
P ++ + D SDDE P+SS F+ K SG+ G Q DEKKPLV ++HQNGST +++
Sbjct: 181 RPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVKNE 240
Query: 241 LQKPAVLSSKRPLPNADSHTPSQISTKKPKLSDSSTPLSSKLVSLKAKQEVDDEDDDIPI 291
+ VL KRPL S Q S KK K+ S++P S K+ K+E+DD+ + +
Sbjct: 241 VPNGKVL-GKRPLEKNSS--ADQSSLKKAKI--SASPTSVKMKQDSVKKEIDDK-GRVLV 275
BLAST of Bhi11G000412 vs. TAIR 10
Match:
AT4G26701.1 (DNA binding;DNA topoisomerase type Is )
HSP 1 Score: 129.0 bits (323), Expect = 1.9e-29
Identity = 60/68 (88.24%), Postives = 64/68 (94.12%), Query Frame = 0
Query: 822 KIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAM 881
KIEK+ERDM+TKEDLKTVALGTSKINY+DPRITVAWCKRHE PIEKIFNKSLL KFAWAM
Sbjct: 2 KIEKMERDMQTKEDLKTVALGTSKINYMDPRITVAWCKRHEAPIEKIFNKSLLEKFAWAM 61
Query: 882 DVDPDFRF 890
DV+P F F
Sbjct: 62 DVEPHFTF 69
BLAST of Bhi11G000412 vs. ExPASy Swiss-Prot
Match:
P30181 (DNA topoisomerase 1 alpha OS=Arabidopsis thaliana OX=3702 GN=TOP1A PE=1 SV=1)
HSP 1 Score: 1023.8 bits (2646), Expect = 1.1e-297
Identity = 576/940 (61.28%), Postives = 689/940 (73.30%), Query Frame = 0
Query: 1 MAVETFGKAPMFDD--DDDEDNMPIVFKRSS--ASRQNQLDSEIKHSQSQRLNKLSGRQG 60
M ET K P+ D+ D +D+ P+ FKR++ AS NQ S + S++ K+S +
Sbjct: 1 MGTETVSK-PVMDNGSGDSDDDKPLAFKRNNTVASNSNQSKSNSQRSKAVPTTKVSPMR- 60
Query: 61 SDICSTNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSPIREPPVKPLGTSLKVSDSMDS 120
S + S NG + +N KTS ++S + S + SP + P + +++K
Sbjct: 61 SPVTSPNGTTPSN--------KTSIVKSSMPSSSSKASPAKSPLRNDMPSTVK------D 120
Query: 121 RSKPLVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGN----------SSQPNKGLVPT 180
RS+ ++K+ + E+ DSEDD+PLS L GN S QP K
Sbjct: 121 RSQ------LQKDQSECKI--EHEDSEDDRPLSSILSGNKGPTSSRQVSSPQPEKKNNGD 180
Query: 181 VPKNKVKKSSGD-SDDEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDK 240
P ++ + D SDDE P+SS F+ K SG+ G Q DEKKPLV ++HQNGST +++
Sbjct: 181 RPLDRASRIIKDESDDETPISSMFRKKIDSGMSGGNQLSNDEKKPLVQKLHQNGSTVKNE 240
Query: 241 LQKPAVLSSKRPLPNADSHTPSQISTKKPKLSDSSTPLSSK--------------LVS-- 300
+ VL KRPL S Q S KK K+S S T + K LVS
Sbjct: 241 VPNGKVL-GKRPLEKNSS--ADQSSLKKAKISASPTSVKMKQDSVKKEIDDKGRVLVSPK 300
Query: 301 LKAKQ-------EVDDEDDDIPISQRMKKMSAPVNKSSSSKQTVS-------------KV 360
+KAKQ DD+DDD+PIS+R K S+ N SS+ + V V
Sbjct: 301 MKAKQLSTREDGTDDDDDDDVPISKRFKSDSSNSNTSSAKPKAVKLNSTSSAAKPKARNV 360
Query: 361 VSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQTHGVKIL 420
VS + K +KK K SKY S+K PS+GDGQKKWTTLVHNGVIFPPPY+ HG+KIL
Sbjct: 361 VSPRSRAMTKNTKKVTKDSKYSTSSKSSPSSGDGQKKWTTLVHNGVIFPPPYKPHGIKIL 420
Query: 421 YKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHVIQNLDDCDFT 480
YKGKPVDLT EQEEVATMFAVM++T+Y KP+F+ENFWNDWR+LLGK HVIQ LDDCDFT
Sbjct: 421 YKGKPVDLTIEQEEVATMFAVMRETDYYTKPQFRENFWNDWRRLLGKKHVIQKLDDCDFT 480
Query: 481 PIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPGLFRG 540
PIY+W+ +EKEKKKQM+TEEKKALKEEK+KQEEKYMWA++DGVKEK+GNFRVEPPGLFRG
Sbjct: 481 PIYEWHLEEKEKKKQMSTEEKKALKEEKMKQEEKYMWAVVDGVKEKIGNFRVEPPGLFRG 540
Query: 541 RGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFWNDPINQ 600
RGEHPKMGKLK+RIHP +IT+NIGKGAPIPECPI GERWKEV+HDNTVTWLAFW DPIN
Sbjct: 541 RGEHPKMGKLKKRIHPCEITLNIGKGAPIPECPIAGERWKEVKHDNTVTWLAFWADPINP 600
Query: 601 KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTRRQIAVATYL 660
KEFKYVFL A SSLKG SDKEKYEKAR L D+I NIR YTK+FT+KD RQIAVATYL
Sbjct: 601 KEFKYVFLGAGSSLKGLSDKEKYEKARNLTDHIDNIRTTYTKNFTAKDVKMRQIAVATYL 660
Query: 661 IDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKYENTVEVELPV 720
IDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN ++F+FLGKDSI+Y NTVEVE V
Sbjct: 661 IDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKIKFDFLGKDSIQYVNTVEVEPLV 720
Query: 721 FKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGTK 780
+KAI QFQ+GKS TDDLFD+LDTSKLNAHLK+L+PGLTAKVFRTYNASITLD+ML++ TK
Sbjct: 721 YKAIGQFQAGKSKTDDLFDELDTSKLNAHLKELVPGLTAKVFRTYNASITLDEMLSQETK 780
Query: 781 DGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKELKIDLDRA 840
DGDV +KI YQ ANKEVAIICNHQRTVSK+HGAQ+ +L +I EL++++KELK +LDRA
Sbjct: 781 DGDVTQKIVVYQKANKEVAIICNHQRTVSKTHGAQIEKLTARIEELKEVLKELKTNLDRA 840
Query: 841 KKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYL 890
KKGKPPL+ +DGK+ R+L P A EKKI+Q +AKIEK+ERDM TKEDLKTVALGTSKINYL
Sbjct: 841 KKGKPPLEGSDGKKIRSLEPNAWEKKIAQQSAKIEKMERDMHTKEDLKTVALGTSKINYL 900
BLAST of Bhi11G000412 vs. ExPASy Swiss-Prot
Match:
Q9FJ79 (DNA topoisomerase 1 beta OS=Arabidopsis thaliana OX=3702 GN=TOP1B PE=1 SV=1)
HSP 1 Score: 1008.1 bits (2605), Expect = 6.4e-293
Identity = 573/941 (60.89%), Postives = 676/941 (71.84%), Query Frame = 0
Query: 1 MAVETFGKAPMFD-----DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRQ 60
MA E F K + + +D+DED++P+VFKR+S ++ ++ +N + +
Sbjct: 1 MATEAFVKPVVPNGHDGYEDEDEDDIPLVFKRNS-------NTAATTNRPSPIN--NAMR 60
Query: 61 GSDICSTNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSPIREPPVKPLGTSLKVSDSMD 120
S I ST P TSP RS S ++S P + S S D
Sbjct: 61 NSAIGSTKSSPPMRS------PLTSPNRSASSSTRSSMMKPALPSSSSVQRSTLKSPLRD 120
Query: 121 SRSKPLVNTAVKKEN----VSIILTQENSDSEDDKPLSIRLKGNS-------SQPNKGLV 180
RS K+ N + ++ DSEDDKPLS RLK +S S +G
Sbjct: 121 DRS-----VVAKERNGFGKAPSVSKSDDEDSEDDKPLSARLKLDSKEVTKQPSSSGRGST 180
Query: 181 PTVPKNKVKKSSGDS--------DDEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVH 240
+ + G S D+ P+SS Q K+ G SK ++K+PLV +
Sbjct: 181 QQAVQKSNMRPQGLSDYTKKKVLDERAPMSSTVQTKTSVGTSSSKPVHIEQKRPLVNNID 240
Query: 241 QNG---STSRDKLQKPAVLSSKRPLPNADSHTPSQISTKKPKLS---------------- 300
+NG T Q PA KRPL S +Q S K+PKLS
Sbjct: 241 RNGLKPKTEGHSSQAPA----KRPLEKGSS--SNQSSVKRPKLSEPARPVKVEQGSHISA 300
Query: 301 ---------DSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKSSSSKQTVSK 360
D+S PL + ++ K++ D DD +PI+ RMK S+ NKSSS+K + SK
Sbjct: 301 TQDAKGKNLDASKPLRANQATV--KEDNSDGDDHVPIASRMKSDSSN-NKSSSAKPSSSK 360
Query: 361 VVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQTHGVKI 420
+++SS + +K K VK SKY KS+K LPS GDGQKKW TL HNGVIFPPPY+ HGVKI
Sbjct: 361 MIASSSRTIAQKPNKWVKDSKYSKSSKSLPS-GDGQKKWKTLQHNGVIFPPPYKRHGVKI 420
Query: 421 LYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHVIQNLDDCDF 480
L++GKPVDLTPEQEEVATMFAVM++TEY KPKF+ENFWNDWRKLLGKNH+I++LDDCDF
Sbjct: 421 LFQGKPVDLTPEQEEVATMFAVMRETEYYKKPKFRENFWNDWRKLLGKNHMIKSLDDCDF 480
Query: 481 TPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPGLFR 540
TPIY+WY +EKE KKQMT EEK+ +KEEKLKQEEKYMWA+LDGV+E++GNFRVEPPGLFR
Sbjct: 481 TPIYEWYMQEKETKKQMTAEEKRIVKEEKLKQEEKYMWAVLDGVRERIGNFRVEPPGLFR 540
Query: 541 GRGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFWNDPIN 600
GRGEHPKMGKLK+RI P DITINIGK APIPECPIPGERWKEV+HDNTVTWLAFW+DPIN
Sbjct: 541 GRGEHPKMGKLKKRIRPCDITINIGKEAPIPECPIPGERWKEVKHDNTVTWLAFWSDPIN 600
Query: 601 QKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTRRQIAVATY 660
KEFKYVFLAASSSLKGQSDKEKYEKAR L ++I +IR AYTKDF +KD T+RQIAVATY
Sbjct: 601 PKEFKYVFLAASSSLKGQSDKEKYEKARKLHNHIGSIRAAYTKDFNNKDVTKRQIAVATY 660
Query: 661 LIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKYENTVEVELP 720
LIDKLALRAGNEKDDDEADTVGCCTLKV NV+ +PPN L+F+FLGKDSI+Y NTVEVE
Sbjct: 661 LIDKLALRAGNEKDDDEADTVGCCTLKVGNVECIPPNKLKFDFLGKDSIQYVNTVEVEPL 720
Query: 721 VFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNRGT 780
V+KAI QFQ+GKS TDDLFD+LDTSKLN HLK+LM GLTAKVFRTYNASITLD ML++ T
Sbjct: 721 VYKAIGQFQAGKSKTDDLFDELDTSKLNTHLKELMAGLTAKVFRTYNASITLDLMLSKET 780
Query: 781 KDGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKELKIDLDR 840
+DGDV EK+ YQ ANKEVAIICNHQRTVSKSHGAQ+ +L KI ELR+ +KEL IDLDR
Sbjct: 781 RDGDVPEKVVVYQQANKEVAIICNHQRTVSKSHGAQVEKLAVKIEELREQIKELNIDLDR 840
Query: 841 AKKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINY 890
AKKG+ PL +DGKRKRNLTPEALEKKI QT KIEK+ERDM+TKED+KTVALGTSKINY
Sbjct: 841 AKKGRTPLMGSDGKRKRNLTPEALEKKIMQTQGKIEKMERDMQTKEDMKTVALGTSKINY 900
BLAST of Bhi11G000412 vs. ExPASy Swiss-Prot
Match:
P93119 (DNA topoisomerase 1 OS=Daucus carota OX=4039 GN=TOP1 PE=2 SV=1)
HSP 1 Score: 840.9 bits (2171), Expect = 1.3e-242
Identity = 483/793 (60.91%), Postives = 575/793 (72.51%), Query Frame = 0
Query: 139 NSDSEDDKPLSIR-----LKGNSSQPNKG------LVPTVPKNKVKKSSGDSDDEIPLSS 198
+ DS+DDKPLS + L GNS G T PK ++ K +DE+ LSS
Sbjct: 32 SEDSDDDKPLSHKLSSGALNGNSHHIRMGSNLSCPSPYTSPKPRIIKG---PEDEMLLSS 91
Query: 199 KFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDKLQKPAVLSSKRPLPNADSHTPS 258
+ Q+ +G+ ++ DE KPL +L+ P+ +KRPL + PS
Sbjct: 92 ELQLNAGAS--NAESSDSDESKPLA-----------SELEDPSSSLNKRPLIQPKNSDPS 151
Query: 259 QISTKKPKLSDSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKSSSSKQTVS 318
+KK KLS+ S P + KL + A+QE DDD IS R KK + P +K S K+
Sbjct: 152 --PSKKAKLSEPSAPTNRKLKT--AEQEEAAADDDPSISNRNKKSTTPASKVSDKKKRPD 211
Query: 319 KVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQTHGVK 378
VS+S+ K V SK +K +L +GQ LVHNGVIFPP Y+ HGVK
Sbjct: 212 --VSASVNK--------VDFSKSLKVPLVLVKGRNGQ----PLVHNGVIFPPLYKPHGVK 271
Query: 379 ILYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHVIQNLDDCD 438
+LY+ PVDLTPEQEEVATMFAVM +TEYM KPKF+ENF +DWRK+LG+ H+IQNL+DCD
Sbjct: 272 MLYRENPVDLTPEQEEVATMFAVMLETEYMTKPKFRENFMSDWRKILGEKHIIQNLEDCD 331
Query: 439 FTPIYDWYQKEKEKKKQMTTEEKKALKEEKLK-QEEKYMWAILDGV-KEKVGNFRVEPPG 498
FTPIY+W+Q+EKEKKKQM+T+EKKA+KE K + ++EKYMWA++DG+ +EKVGNFRV
Sbjct: 332 FTPIYEWHQREKEKKKQMSTDEKKAIKEGKDETRKEKYMWAVVDGMSREKVGNFRVGTTR 391
Query: 499 LFRGRGEHPKMGKLKRRIHPS-DITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFWN 558
+ + K ++K+ ++ DITINIGK APIPE PIPGERWKE+RHDNTVTWLAFWN
Sbjct: 392 VVQRSWRASKDPQVKKNVYSQIDITINIGKDAPIPEPPIPGERWKEIRHDNTVTWLAFWN 451
Query: 559 DPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIR-NAYTKDFTSKDSTRRQI 618
DPIN KEFKYVFLAASSSLKG+ +EKYEK+R LKDYI+ IR AYTKDF SKDS +RQI
Sbjct: 452 DPINPKEFKYVFLAASSSLKGKVTREKYEKSRKLKDYIEGIRAAAYTKDFASKDSKKRQI 511
Query: 619 AVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKYENTV 678
AVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENV+ PN+L+F+FLGKDSI+Y+N V
Sbjct: 512 AVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVETKRPNILKFDFLGKDSIRYQNEV 571
Query: 679 EVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDM 738
EVE VF AI QF+SGK G DDLFD+LDTSKLNAHLK+LMPGLTAKVFRTYNASITLD+M
Sbjct: 572 EVEPRVFSAIEQFRSGKEGGDDLFDQLDTSKLNAHLKELMPGLTAKVFRTYNASITLDEM 631
Query: 739 -----LNRGTKDGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI 798
L+R TK GDV EK+ YQHANKEVAIICNHQRTVSKSH AQM RLNEKI EL+ +
Sbjct: 632 VRCKLLSRETKGGDVAEKVVVYQHANKEVAIICNHQRTVSKSHSAQMVRLNEKIEELKGL 691
Query: 799 VKELKIDLDRAKKGKPPLKDADGKRKRNLTPE----------------------ALEKKI 858
+KEL+ DL R KGKPPLK++DGK KRNL + ++ KKI
Sbjct: 692 LKELQEDLTRVNKGKPPLKNSDGKPKRNLNLKRYKGKLLKLIQKLRKWSETRRLSVTKKI 751
Query: 859 SQTNAKIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAK 890
+QTN KIEK+ERD +TKEDLKTVALGTSKINYLDPRITVAWCKRH+VPIEKIFNKSLLAK
Sbjct: 752 AQTNTKIEKMERDKETKEDLKTVALGTSKINYLDPRITVAWCKRHDVPIEKIFNKSLLAK 790
BLAST of Bhi11G000412 vs. ExPASy Swiss-Prot
Match:
Q54RC3 (DNA topoisomerase 1 OS=Dictyostelium discoideum OX=44689 GN=top1 PE=3 SV=1)
HSP 1 Score: 527.3 bits (1357), Expect = 3.3e-148
Identity = 349/867 (40.25%), Postives = 501/867 (57.79%), Query Frame = 0
Query: 71 KRMTSLPKTSPMRSEIGSQKASKSPIREPPVKPLGTSLKVSDSMDSRSKPLVNTAVKKEN 130
K++T PK +P+ + + K PI P KP+ +S SDS DS S + + ++
Sbjct: 59 KKLTPTPKPAPVTPKPTAAVNGKKPI--PKKKPVDSS-SDSDSSDSSSD---SDSDSSDS 118
Query: 131 VSIILTQENSDSEDDKPLSIRLKGNSSQPNK--GLVPTVPKNKVKKSSGDSDDEIPLSSK 190
S + ++S S+ D P + K S PNK G V KN K ++ + + SK
Sbjct: 119 DSDSSSSDSSSSDSDVP---KKKPISKIPNKPSGTVSKTSKNSPKTTTTTTTTTTTVKSK 178
Query: 191 FQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDKLQKPAVLSSKRPLPNADSHTPSQ 250
K S D + S S + +KP SK+P + +
Sbjct: 179 PAPKVKPEQSSSDDSSSDSSD---DSSDDSSSDSSSEEEKPKKKPSKKP-----AAVKKE 238
Query: 251 ISTKKPKLSDSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKSSSSKQTVSK 310
I+ KK T ++K +K + EV E ++++KK + + K T K
Sbjct: 239 ITVKK------ETTTTTKKTPVKKEVEVKKE-TKTTTTKKVKKEESD-DDGEDEKTTTKK 298
Query: 311 VVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQTHGVKI 370
++ +K ++ + ++ + V D KWTTL+HNG +FPP Y HG+K
Sbjct: 299 KTTTRKRKLKEEEEGEEEEEEGVNKWWDKEDDEDDGTKWTTLIHNGPVFPPAYVPHGIKF 358
Query: 371 LYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLL--GKNHVIQNLDDC 430
+Y GK V LTP+QEEVAT +A +T+++ K FK+NF+ +++ LL + +++ +D C
Sbjct: 359 MYDGKVVKLTPKQEEVATFYANYLETDHVKKQAFKDNFFKEFKGLLTSEQKEIVKKMDKC 418
Query: 431 DFTPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFRVEPPGL 490
DF+ I+ + +KE++K + EEK A EK K +EK+ +A +DG KEKVGN+ +EPPGL
Sbjct: 419 DFSHIHTHLKTKKEQRKARSKEEKDAETAEKKKIQEKHGFATIDGFKEKVGNYMIEPPGL 478
Query: 491 FRGRGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWLAFWNDP 550
F GRG+HPK G LK+RI PSD+T+NIG+G IP PIPGE+WKE+ H+NTVTWLAFW +
Sbjct: 479 FLGRGQHPKTGMLKQRIMPSDVTLNIGEGEVIPPSPIPGEKWKEIVHNNTVTWLAFWRES 538
Query: 551 INQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTRRQIAVA 610
+N FKYV+LA SS +KGQ+D++K+E AR LK+ I +IR Y K F + + +RQ+AVA
Sbjct: 539 VN-GGFKYVWLAPSSKIKGQADRKKFEVARGLKNCIGDIRKGYQKSFNDESTEKRQLAVA 598
Query: 611 TYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKYENTVEVE 670
YLID LALR GNEK +D+ADTVGCC+L+VE++K+ N + +FLGKDS++Y NTV++
Sbjct: 599 LYLIDFLALRVGNEKGEDQADTVGCCSLRVEHIKLESNNTITLDFLGKDSMRYLNTVQIR 658
Query: 671 LPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITLDDMLNR 730
+K + F GK TD LFD L ++LN HLK M GL+AKVFRTYNASITL L++
Sbjct: 659 EDAYKVLKTFVKGKKPTDLLFDSLTVTQLNNHLKKQMTGLSAKVFRTYNASITLQKELDK 718
Query: 731 -GTKD-GDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVKELKI 790
TK+ + EKI Y N+EVAI+CNHQ+ K+ M++++EKI ELRD+
Sbjct: 719 MDTKEFQSIDEKILFYNRCNREVAILCNHQKAPPKNLAETMSKIDEKIQELRDLQ----- 778
Query: 791 DLDRAKKGKPP-------------------------LKDADGKR--------------KR 850
DL R K GK P KD D ++ K
Sbjct: 779 DLCRIKIGKTPKSQQEDIESREAERLPKLIQLAITNKKDKDKEKTDKEISFDVKAAFEKS 838
Query: 851 NLTPEA---LEKKISQTNAKIEKIERDMKTKEDLKTVALGTSKINYLDPRITVAWCKRHE 890
P+A +++KI++ +I+K E K++LKTVALGTSKINYLDPRITVAWCK+++
Sbjct: 839 RTLPDAKGKIKEKIAKYEEQIKKQEILKTGKDELKTVALGTSKINYLDPRITVAWCKKNK 893
BLAST of Bhi11G000412 vs. ExPASy Swiss-Prot
Match:
P07799 (DNA topoisomerase 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=top1 PE=1 SV=2)
HSP 1 Score: 523.9 bits (1348), Expect = 3.6e-147
Identity = 341/846 (40.31%), Postives = 472/846 (55.79%), Query Frame = 0
Query: 129 ENVSIILTQENSDSEDDKPLSIRLKGNSSQPNKGLVPTVPKNKVKKSSGDSDDEIPL--- 188
+ +S+ + E SDS ++ PLS L NK KS D DD IP+
Sbjct: 23 KRISMKESDEESDSSENHPLSESL-----------------NKKSKSESDEDD-IPIRKR 82
Query: 189 --SSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDKLQKPAVLSSKRPLPNADS 248
SSK M + S +K G NG K K AV+ +
Sbjct: 83 RASSKKNMSNSSSKKRAKVMG-------------NGGLKNGK--KTAVVKEEEDF----- 142
Query: 249 HTPSQISTKKPKLSDSSTPLSSKLVSLKA-KQEVDDEDDDIPISQRMKKMSAPVNKSSSS 308
++I+ PK S SK + A K+E D DD +P+ P
Sbjct: 143 ---NEIAKPSPKHKRVSKANGSKNGAKSAVKKEESDTDDSVPLRAVSTVSLTPY------ 202
Query: 309 KQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPPYQ 368
K + S++ ++ + + K+ S + D +KWTTL HNGVIF PPY+
Sbjct: 203 KSELPSGASTTQNRSPNDEEDEDEDYKWWTSENI-----DDTQKWTTLEHNGVIFAPPYE 262
Query: 369 --THGVKILYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGK---N 428
VK++Y G PV+L PE EEVA +A M +T++ P F++NF+ D+ K+ + N
Sbjct: 263 PLPKNVKLIYDGNPVNLPPEAEEVAGFYAAMLETDHAKNPVFQDNFFRDFLKVCDECNFN 322
Query: 429 HVIQNLDDCDFTPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVG 488
H I+ CDFT ++ +++++E+KK M E+KKA+K++K ++EEKY W ILDG KEKVG
Sbjct: 323 HNIKEFSKCDFTQMFHHFEQKREEKKSMPKEQKKAIKQKKDEEEEKYKWCILDGRKEKVG 382
Query: 489 NFRVEPPGLFRGRGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTV 548
NFR+EPPGLFRGRG HPK G LKRR++P ITINIG+G P+PE P+PG +W EV+HDNTV
Sbjct: 383 NFRIEPPGLFRGRGSHPKTGSLKRRVYPEQITINIGEGVPVPE-PLPGHQWAEVKHDNTV 442
Query: 549 TWLAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKD 608
TWLA W++ IN KYVFLAA SSLKGQSD +KYEK+R LKDYI +IR Y KD S+
Sbjct: 443 TWLATWHENIN-NNVKYVFLAAGSSLKGQSDLKKYEKSRKLKDYIDDIRKGYRKDLKSEL 502
Query: 609 STRRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSI 668
+ RQ A YLID ALRAGNEK +DEADTVGCC+L+ E+V + PP + F+FLGKDSI
Sbjct: 503 TVERQRGTAMYLIDVFALRAGNEKGEDEADTVGCCSLRYEHVTLKPPRTVVFDFLGKDSI 562
Query: 669 KYENTVEVELPVFKAITQF-QSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNA 728
+Y N VEV+ VFK + F + K D +FD+L T+ LN +L LM GL+AKVFRTYNA
Sbjct: 563 RYYNEVEVDPQVFKNLKIFKRPPKKEGDLIFDRLSTNSLNKYLTSLMDGLSAKVFRTYNA 622
Query: 729 SITLDDMLNRGTKDGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISEL- 788
S T+ + L + K+ + +KI Y AN+ VAI+CNHQR+V+K+H QM R E+I L
Sbjct: 623 SYTMAEELKKMPKNLTLADKILFYNRANRTVAILCNHQRSVTKNHDVQMERFAERIKALQ 682
Query: 789 --RDIVKELKIDLD-RAKKGKPPL------------------------------------ 848
R ++++ ++L+ + K KP L
Sbjct: 683 YQRMRLRKMMLNLEPKLAKSKPELLAKEEGITDSWIVKHHETLYELEKEKIKKKFDRENE 742
Query: 849 -----------------------------KDADGKRKR----NLTPEALEKKISQTNAKI 890
DA+ K K+ + E LEK++++ N +I
Sbjct: 743 KLAAEDPKSVLPESELEVRLKAADELKKALDAELKSKKVDPGRSSMEQLEKRLNKLNERI 802
BLAST of Bhi11G000412 vs. NCBI nr
Match:
XP_038904656.1 (DNA topoisomerase 1 alpha-like isoform X1 [Benincasa hispida] >XP_038904657.1 DNA topoisomerase 1 alpha-like isoform X1 [Benincasa hispida])
HSP 1 Score: 1729.1 bits (4477), Expect = 0.0e+00
Identity = 889/889 (100.00%), Postives = 889/889 (100.00%), Query Frame = 0
Query: 1 MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRQGSDIC 60
MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRQGSDIC
Sbjct: 1 MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRQGSDIC 60
Query: 61 STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSPIREPPVKPLGTSLKVSDSMDSRSKP 120
STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSPIREPPVKPLGTSLKVSDSMDSRSKP
Sbjct: 61 STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSPIREPPVKPLGTSLKVSDSMDSRSKP 120
Query: 121 LVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGNSSQPNKGLVPTVPKNKVKKSSGDSD 180
LVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGNSSQPNKGLVPTVPKNKVKKSSGDSD
Sbjct: 121 LVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGNSSQPNKGLVPTVPKNKVKKSSGDSD 180
Query: 181 DEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDKLQKPAVLSSKRPLPN 240
DEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDKLQKPAVLSSKRPLPN
Sbjct: 181 DEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDKLQKPAVLSSKRPLPN 240
Query: 241 ADSHTPSQISTKKPKLSDSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKSS 300
ADSHTPSQISTKKPKLSDSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKSS
Sbjct: 241 ADSHTPSQISTKKPKLSDSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKSS 300
Query: 301 SSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360
SSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP
Sbjct: 301 SSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360
Query: 361 YQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHVI 420
YQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHVI 420
Query: 421 QNLDDCDFTPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480
QNLDDCDFTPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR
Sbjct: 421 QNLDDCDFTPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480
Query: 481 VEPPGLFRGRGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL 540
VEPPGLFRGRGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL
Sbjct: 481 VEPPGLFRGRGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL 540
Query: 541 AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTR 600
AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTR
Sbjct: 541 AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTR 600
Query: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKYE 660
RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKYE
Sbjct: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKYE 660
Query: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720
NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL
Sbjct: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720
Query: 721 DDMLNRGTKDGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVK 780
DDMLNRGTKDGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVK
Sbjct: 721 DDMLNRGTKDGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVK 780
Query: 781 ELKIDLDRAKKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
ELKIDLDRAKKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA
Sbjct: 781 ELKIDLDRAKKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
Query: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 890
LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 889
BLAST of Bhi11G000412 vs. NCBI nr
Match:
XP_008449103.1 (PREDICTED: DNA topoisomerase 1-like [Cucumis melo] >XP_008449104.1 PREDICTED: DNA topoisomerase 1-like [Cucumis melo])
HSP 1 Score: 1618.2 bits (4189), Expect = 0.0e+00
Identity = 834/890 (93.71%), Postives = 858/890 (96.40%), Query Frame = 0
Query: 1 MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRQGSDIC 60
MAVETFGKAPMF DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQ+QRLNKL GRQGSD+C
Sbjct: 1 MAVETFGKAPMF-DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLC 60
Query: 61 STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASK-SPIREPPVKPLGTSLKVSDSMDSRSK 120
STNGQSSNNQKRMTSLPKTSPMRSEIGSQKA K SPI+EP K LG SLK SDSMDSR K
Sbjct: 61 STNGQSSNNQKRMTSLPKTSPMRSEIGSQKAPKSSPIQEPLTKSLGISLKASDSMDSRPK 120
Query: 121 PLVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGNSSQPNKGLVPTVPKNKVKKSSGDS 180
LVN AVK+ENVSI +QENSDSEDDKPLSIRLKGNS+QPNKGLV T+PKNKVKKSSGDS
Sbjct: 121 NLVNAAVKEENVSINPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTIPKNKVKKSSGDS 180
Query: 181 DDEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDKLQKPAVLSSKRPLP 240
DDE+PLSSKFQMK SG +GSKQ GFDEKKPLVP+VHQNGSTSRDKLQKPAVLSSKRPLP
Sbjct: 181 DDEVPLSSKFQMKHASGPMGSKQLGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP 240
Query: 241 NADSHTPSQISTKKPKLSDSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKS 300
N DSH+PSQ STKKPKLSDSS+PLSSKLVSLKAKQEVDD+DDDIPISQR+KK A VNKS
Sbjct: 241 NEDSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDDIPISQRLKKTPATVNKS 300
Query: 301 SSSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
SSSK TVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP
Sbjct: 301 SSSKPTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
Query: 361 PYQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHV 420
PYQTHGVK+LYKGKPVDLTP+QEEVATMFAVMKDT+YMLKPKFKENFWNDWRKLLGKNHV
Sbjct: 361 PYQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420
Query: 421 IQNLDDCDFTPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF 480
IQNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF
Sbjct: 421 IQNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF 480
Query: 481 RVEPPGLFRGRGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW 540
RVEPPGLFRGRGEHPKMGKLKRRI PSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW
Sbjct: 481 RVEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW 540
Query: 541 LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDST 600
LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKAR+LKDYIKNIRNAYTKDFTSKD T
Sbjct: 541 LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARVLKDYIKNIRNAYTKDFTSKDPT 600
Query: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKY 660
RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVK VPPNLLEFNFLGKDSI+Y
Sbjct: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRY 660
Query: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASIT 720
ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASIT
Sbjct: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASIT 720
Query: 721 LDDMLNRGTKDGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIV 780
LDDMLNRGTKDG+V EKI TYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI+
Sbjct: 721 LDDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIL 780
Query: 781 KELKIDLDRAKKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV 840
KELKIDLDRAKKGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV
Sbjct: 781 KELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV 840
Query: 841 ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 890
ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 889
BLAST of Bhi11G000412 vs. NCBI nr
Match:
XP_004149585.1 (DNA topoisomerase 1 alpha [Cucumis sativus] >XP_011650506.1 DNA topoisomerase 1 alpha [Cucumis sativus] >KGN56068.1 hypothetical protein Csa_010762 [Cucumis sativus])
HSP 1 Score: 1610.9 bits (4170), Expect = 0.0e+00
Identity = 830/890 (93.26%), Postives = 856/890 (96.18%), Query Frame = 0
Query: 1 MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRQGSDIC 60
MAVETFGKAPMF DDDDEDNMPIVFKR+SASRQNQLDSEIKHSQ+QRL+KL GRQGSD+C
Sbjct: 1 MAVETFGKAPMF-DDDDEDNMPIVFKRTSASRQNQLDSEIKHSQAQRLSKLPGRQGSDLC 60
Query: 61 STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASK-SPIREPPVKPLGTSLKVSDSMDSRSK 120
STNGQSSNNQKRMTSLPKTSPMRSEIGSQKAS+ SPI EP K LGTSLK S SMDSR K
Sbjct: 61 STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASRSSPIPEPLTKSLGTSLKASHSMDSRPK 120
Query: 121 PLVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGNSSQPNKGLVPTVPKNKVKKSSGDS 180
LVN VK+ENVSI +QENSDSEDDKPLSIRLKGN +QPNKGLV TVPKNKVKKS DS
Sbjct: 121 SLVNAVVKEENVSINPSQENSDSEDDKPLSIRLKGNPNQPNKGLVSTVPKNKVKKSLADS 180
Query: 181 DDEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDKLQKPAVLSSKRPLP 240
DDE+PLSSKFQMK SGVLGSKQHGFDEKKPLVP+VHQNGSTSRDKLQKPAVLSSKRPLP
Sbjct: 181 DDEVPLSSKFQMKHASGVLGSKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP 240
Query: 241 NADSHTPSQISTKKPKLSDSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKS 300
N DSH+PSQ TKKPKL+DSS+PL+SKLVSLKAKQEVDD+DDDIPISQRMKK+SA VNKS
Sbjct: 241 NEDSHSPSQFPTKKPKLTDSSSPLNSKLVSLKAKQEVDDDDDDIPISQRMKKVSATVNKS 300
Query: 301 SSSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
SSSK T+SKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP
Sbjct: 301 SSSKPTISKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
Query: 361 PYQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHV 420
PYQTHGVK+LYKGKPVDLTP QEEVATMFAVMKDT+YMLKPKFKENFWNDWRKLLGKNHV
Sbjct: 361 PYQTHGVKMLYKGKPVDLTPPQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420
Query: 421 IQNLDDCDFTPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF 480
IQNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKA+KEEKLKQEEKYMWAILDGVKEKVGNF
Sbjct: 421 IQNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKAVKEEKLKQEEKYMWAILDGVKEKVGNF 480
Query: 481 RVEPPGLFRGRGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW 540
RVEPPGLFRGRGEHPKMGKLKRRI PSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW
Sbjct: 481 RVEPPGLFRGRGEHPKMGKLKRRICPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW 540
Query: 541 LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDST 600
LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKD T
Sbjct: 541 LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDPT 600
Query: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKY 660
RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVK VPPNLLEFNFLGKDSI+Y
Sbjct: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRY 660
Query: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASIT 720
ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASIT
Sbjct: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASIT 720
Query: 721 LDDMLNRGTKDGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIV 780
LDDMLNRGTK+G+V EKI TYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI+
Sbjct: 721 LDDMLNRGTKNGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIL 780
Query: 781 KELKIDLDRAKKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV 840
KELKIDLDRAKKGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV
Sbjct: 781 KELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV 840
Query: 841 ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 890
ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 889
BLAST of Bhi11G000412 vs. NCBI nr
Match:
KAA0065816.1 (DNA topoisomerase 1-like [Cucumis melo var. makuwa] >TYJ96555.1 DNA topoisomerase 1-like [Cucumis melo var. makuwa])
HSP 1 Score: 1607.0 bits (4160), Expect = 0.0e+00
Identity = 834/908 (91.85%), Postives = 858/908 (94.49%), Query Frame = 0
Query: 1 MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRQGSDIC 60
MAVETFGKAPMF DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQ+QRLNKL GRQGSD+C
Sbjct: 1 MAVETFGKAPMF-DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLC 60
Query: 61 STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASK-SPIREPPVKPLGTSLKVSDSMDSRSK 120
STNGQSSNNQKRMTSLPKTSPMRSEIGSQKA K SPI+EP K LG SLK SDSMDSR K
Sbjct: 61 STNGQSSNNQKRMTSLPKTSPMRSEIGSQKAPKSSPIQEPLTKSLGISLKASDSMDSRPK 120
Query: 121 PLVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGNSSQPNKGLVPTVPKNKVKKSSGDS 180
LVN AVK+ENVSI +QENSDSEDDKPLSIRLKGNS+QPNKGLV T+PKNKVKKSSGDS
Sbjct: 121 NLVNAAVKEENVSINPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTIPKNKVKKSSGDS 180
Query: 181 DDEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDKLQKPAVLSSKRPLP 240
DDE+PLSSKFQMK SG +GSKQ GFDEKKPLVP+VHQNGSTSRDKLQKPAVLSSKRPLP
Sbjct: 181 DDEVPLSSKFQMKHASGPMGSKQLGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP 240
Query: 241 NADSHTPSQISTKKPKLSDSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKS 300
N DSH+PSQ STKKPKLSDSS+PLSSKLVSLKAKQEVDD+DDDIPISQR+KK A VNKS
Sbjct: 241 NEDSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDDIPISQRLKKTPATVNKS 300
Query: 301 SSSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
SSSK TVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP
Sbjct: 301 SSSKPTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
Query: 361 PYQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHV 420
PYQTHGVK+LYKGKPVDLTP+QEEVATMFAVMKDT+YMLKPKFKENFWNDWRKLLGKNHV
Sbjct: 361 PYQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420
Query: 421 IQNLDDCDFTPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF 480
IQNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF
Sbjct: 421 IQNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF 480
Query: 481 RVEPPGLFRGRGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW 540
RVEPPGLFRGRGEHPKMGKLKRRI PSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW
Sbjct: 481 RVEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW 540
Query: 541 LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDST 600
LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKAR+LKDYIKNIRNAYTKDFTSKD T
Sbjct: 541 LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARVLKDYIKNIRNAYTKDFTSKDPT 600
Query: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKY 660
RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVK VPPNLLEFNFLGKDSI+Y
Sbjct: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRY 660
Query: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASIT 720
ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASIT
Sbjct: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASIT 720
Query: 721 LDDMLNRGTKDGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIV 780
LDDMLNRGTKDG+V EKI TYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI+
Sbjct: 721 LDDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIL 780
Query: 781 KELKIDLDRAKKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV 840
KELKIDLDRAKKGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV
Sbjct: 781 KELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV 840
Query: 841 ALGTSKINYLDPRITVAWCKRHEVPIEK------------------IFNKSLLAKFAWAM 890
ALGTSKINYLDPRITVAWCKRHEVPIEK IFNKSLLAKFAWAM
Sbjct: 841 ALGTSKINYLDPRITVAWCKRHEVPIEKFKNFLLIFVIHNHNLLPQIFNKSLLAKFAWAM 900
BLAST of Bhi11G000412 vs. NCBI nr
Match:
XP_023553297.1 (DNA topoisomerase 1 beta-like [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1577.0 bits (4082), Expect = 0.0e+00
Identity = 815/889 (91.68%), Postives = 845/889 (95.05%), Query Frame = 0
Query: 1 MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRQGSDIC 60
MAVETFGKAPMF DDDDEDNMPIVFKRSSASRQNQL+SE+KHSQSQRLN+LSGR GSD C
Sbjct: 1 MAVETFGKAPMF-DDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDAC 60
Query: 61 STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSPIREPPVKPLGTSLKVSDSMDSRSKP 120
S NGQSSN QKRMTSLPKTSPMR EIGSQKASKS I+EP VK LGT+LKVSDSMDSR K
Sbjct: 61 SANGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQ 120
Query: 121 LVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGNSSQPNKGLVPTVPKNKVKKSSGDSD 180
L N AVK+EN SI TQ+NSDSEDDKPLSIRLKGNS+ P+KG +PTV KNK+KKSS DSD
Sbjct: 121 LANAAVKEENDSIHPTQDNSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSD 180
Query: 181 DEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDKLQKPAVLSSKRPLPN 240
DE+PLSSKFQMKS SGVLG +QHGFDEKKPL +VHQNGSTSRDKLQKPAV+SSKRPLPN
Sbjct: 181 DEVPLSSKFQMKSASGVLGGRQHGFDEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPN 240
Query: 241 ADSHTPSQISTKKPKLSDSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKSS 300
ADSH+ SQIS+KKPKLSDSSTPLS+ VSLKAKQEV D+DD IPISQRM KMSA VNKSS
Sbjct: 241 ADSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVADDDDHIPISQRM-KMSAAVNKSS 300
Query: 301 SSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360
SK+TVSKV SSSIKKT KKSKKPVKKSKYVKSTKLLPS+GDGQKKWTTLVHNGVIFPPP
Sbjct: 301 GSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPP 360
Query: 361 YQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHVI 420
YQTHGVKIL+KGKPVDLTPEQEEVATMFAVMKDT+YM KPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420
Query: 421 QNLDDCDFTPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480
QNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR
Sbjct: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480
Query: 481 VEPPGLFRGRGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL 540
VEPPGLFRGRGEHPKMGKLK+RI PSDITINIGK APIPECPIPG+RWKEVRHDNTVTWL
Sbjct: 481 VEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWL 540
Query: 541 AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTR 600
AFWNDPIN KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRN+YTKDFTSKD TR
Sbjct: 541 AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTR 600
Query: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKYE 660
RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVK VPPNLLEFNFLGKDSIKYE
Sbjct: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE 660
Query: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720
NTVEVELPVFKAITQFQSGKSG+DDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL
Sbjct: 661 NTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720
Query: 721 DDMLNRGTKDGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVK 780
DDMLNRGTKDG+V EKI TYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVK
Sbjct: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVK 780
Query: 781 ELKIDLDRAKKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
ELKIDLDRAKKGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA
Sbjct: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
Query: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 890
LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887
BLAST of Bhi11G000412 vs. ExPASy TrEMBL
Match:
A0A1S3BM72 (DNA topoisomerase I OS=Cucumis melo OX=3656 GN=LOC103491068 PE=3 SV=1)
HSP 1 Score: 1618.2 bits (4189), Expect = 0.0e+00
Identity = 834/890 (93.71%), Postives = 858/890 (96.40%), Query Frame = 0
Query: 1 MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRQGSDIC 60
MAVETFGKAPMF DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQ+QRLNKL GRQGSD+C
Sbjct: 1 MAVETFGKAPMF-DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLC 60
Query: 61 STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASK-SPIREPPVKPLGTSLKVSDSMDSRSK 120
STNGQSSNNQKRMTSLPKTSPMRSEIGSQKA K SPI+EP K LG SLK SDSMDSR K
Sbjct: 61 STNGQSSNNQKRMTSLPKTSPMRSEIGSQKAPKSSPIQEPLTKSLGISLKASDSMDSRPK 120
Query: 121 PLVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGNSSQPNKGLVPTVPKNKVKKSSGDS 180
LVN AVK+ENVSI +QENSDSEDDKPLSIRLKGNS+QPNKGLV T+PKNKVKKSSGDS
Sbjct: 121 NLVNAAVKEENVSINPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTIPKNKVKKSSGDS 180
Query: 181 DDEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDKLQKPAVLSSKRPLP 240
DDE+PLSSKFQMK SG +GSKQ GFDEKKPLVP+VHQNGSTSRDKLQKPAVLSSKRPLP
Sbjct: 181 DDEVPLSSKFQMKHASGPMGSKQLGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP 240
Query: 241 NADSHTPSQISTKKPKLSDSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKS 300
N DSH+PSQ STKKPKLSDSS+PLSSKLVSLKAKQEVDD+DDDIPISQR+KK A VNKS
Sbjct: 241 NEDSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDDIPISQRLKKTPATVNKS 300
Query: 301 SSSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
SSSK TVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP
Sbjct: 301 SSSKPTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
Query: 361 PYQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHV 420
PYQTHGVK+LYKGKPVDLTP+QEEVATMFAVMKDT+YMLKPKFKENFWNDWRKLLGKNHV
Sbjct: 361 PYQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420
Query: 421 IQNLDDCDFTPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF 480
IQNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF
Sbjct: 421 IQNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF 480
Query: 481 RVEPPGLFRGRGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW 540
RVEPPGLFRGRGEHPKMGKLKRRI PSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW
Sbjct: 481 RVEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW 540
Query: 541 LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDST 600
LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKAR+LKDYIKNIRNAYTKDFTSKD T
Sbjct: 541 LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARVLKDYIKNIRNAYTKDFTSKDPT 600
Query: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKY 660
RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVK VPPNLLEFNFLGKDSI+Y
Sbjct: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRY 660
Query: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASIT 720
ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASIT
Sbjct: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASIT 720
Query: 721 LDDMLNRGTKDGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIV 780
LDDMLNRGTKDG+V EKI TYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI+
Sbjct: 721 LDDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIL 780
Query: 781 KELKIDLDRAKKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV 840
KELKIDLDRAKKGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV
Sbjct: 781 KELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV 840
Query: 841 ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 890
ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 889
BLAST of Bhi11G000412 vs. ExPASy TrEMBL
Match:
A0A0A0L2W2 (DNA topoisomerase I OS=Cucumis sativus OX=3659 GN=Csa_3G063680 PE=3 SV=1)
HSP 1 Score: 1610.9 bits (4170), Expect = 0.0e+00
Identity = 830/890 (93.26%), Postives = 856/890 (96.18%), Query Frame = 0
Query: 1 MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRQGSDIC 60
MAVETFGKAPMF DDDDEDNMPIVFKR+SASRQNQLDSEIKHSQ+QRL+KL GRQGSD+C
Sbjct: 1 MAVETFGKAPMF-DDDDEDNMPIVFKRTSASRQNQLDSEIKHSQAQRLSKLPGRQGSDLC 60
Query: 61 STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASK-SPIREPPVKPLGTSLKVSDSMDSRSK 120
STNGQSSNNQKRMTSLPKTSPMRSEIGSQKAS+ SPI EP K LGTSLK S SMDSR K
Sbjct: 61 STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASRSSPIPEPLTKSLGTSLKASHSMDSRPK 120
Query: 121 PLVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGNSSQPNKGLVPTVPKNKVKKSSGDS 180
LVN VK+ENVSI +QENSDSEDDKPLSIRLKGN +QPNKGLV TVPKNKVKKS DS
Sbjct: 121 SLVNAVVKEENVSINPSQENSDSEDDKPLSIRLKGNPNQPNKGLVSTVPKNKVKKSLADS 180
Query: 181 DDEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDKLQKPAVLSSKRPLP 240
DDE+PLSSKFQMK SGVLGSKQHGFDEKKPLVP+VHQNGSTSRDKLQKPAVLSSKRPLP
Sbjct: 181 DDEVPLSSKFQMKHASGVLGSKQHGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP 240
Query: 241 NADSHTPSQISTKKPKLSDSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKS 300
N DSH+PSQ TKKPKL+DSS+PL+SKLVSLKAKQEVDD+DDDIPISQRMKK+SA VNKS
Sbjct: 241 NEDSHSPSQFPTKKPKLTDSSSPLNSKLVSLKAKQEVDDDDDDIPISQRMKKVSATVNKS 300
Query: 301 SSSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
SSSK T+SKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP
Sbjct: 301 SSSKPTISKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
Query: 361 PYQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHV 420
PYQTHGVK+LYKGKPVDLTP QEEVATMFAVMKDT+YMLKPKFKENFWNDWRKLLGKNHV
Sbjct: 361 PYQTHGVKMLYKGKPVDLTPPQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420
Query: 421 IQNLDDCDFTPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF 480
IQNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKA+KEEKLKQEEKYMWAILDGVKEKVGNF
Sbjct: 421 IQNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKAVKEEKLKQEEKYMWAILDGVKEKVGNF 480
Query: 481 RVEPPGLFRGRGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW 540
RVEPPGLFRGRGEHPKMGKLKRRI PSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW
Sbjct: 481 RVEPPGLFRGRGEHPKMGKLKRRICPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW 540
Query: 541 LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDST 600
LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKD T
Sbjct: 541 LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDPT 600
Query: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKY 660
RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVK VPPNLLEFNFLGKDSI+Y
Sbjct: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRY 660
Query: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASIT 720
ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASIT
Sbjct: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASIT 720
Query: 721 LDDMLNRGTKDGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIV 780
LDDMLNRGTK+G+V EKI TYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI+
Sbjct: 721 LDDMLNRGTKNGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIL 780
Query: 781 KELKIDLDRAKKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV 840
KELKIDLDRAKKGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV
Sbjct: 781 KELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV 840
Query: 841 ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 890
ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 ALGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 889
BLAST of Bhi11G000412 vs. ExPASy TrEMBL
Match:
A0A5A7VHI9 (DNA topoisomerase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G002170 PE=3 SV=1)
HSP 1 Score: 1607.0 bits (4160), Expect = 0.0e+00
Identity = 834/908 (91.85%), Postives = 858/908 (94.49%), Query Frame = 0
Query: 1 MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRQGSDIC 60
MAVETFGKAPMF DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQ+QRLNKL GRQGSD+C
Sbjct: 1 MAVETFGKAPMF-DDDDEDNMPIVFKRSSASRQNQLDSEIKHSQAQRLNKLPGRQGSDLC 60
Query: 61 STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASK-SPIREPPVKPLGTSLKVSDSMDSRSK 120
STNGQSSNNQKRMTSLPKTSPMRSEIGSQKA K SPI+EP K LG SLK SDSMDSR K
Sbjct: 61 STNGQSSNNQKRMTSLPKTSPMRSEIGSQKAPKSSPIQEPLTKSLGISLKASDSMDSRPK 120
Query: 121 PLVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGNSSQPNKGLVPTVPKNKVKKSSGDS 180
LVN AVK+ENVSI +QENSDSEDDKPLSIRLKGNS+QPNKGLV T+PKNKVKKSSGDS
Sbjct: 121 NLVNAAVKEENVSINPSQENSDSEDDKPLSIRLKGNSNQPNKGLVSTIPKNKVKKSSGDS 180
Query: 181 DDEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDKLQKPAVLSSKRPLP 240
DDE+PLSSKFQMK SG +GSKQ GFDEKKPLVP+VHQNGSTSRDKLQKPAVLSSKRPLP
Sbjct: 181 DDEVPLSSKFQMKHASGPMGSKQLGFDEKKPLVPKVHQNGSTSRDKLQKPAVLSSKRPLP 240
Query: 241 NADSHTPSQISTKKPKLSDSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKS 300
N DSH+PSQ STKKPKLSDSS+PLSSKLVSLKAKQEVDD+DDDIPISQR+KK A VNKS
Sbjct: 241 NEDSHSPSQFSTKKPKLSDSSSPLSSKLVSLKAKQEVDDDDDDIPISQRLKKTPATVNKS 300
Query: 301 SSSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
SSSK TVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP
Sbjct: 301 SSSKPTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPP 360
Query: 361 PYQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHV 420
PYQTHGVK+LYKGKPVDLTP+QEEVATMFAVMKDT+YMLKPKFKENFWNDWRKLLGKNHV
Sbjct: 361 PYQTHGVKMLYKGKPVDLTPQQEEVATMFAVMKDTDYMLKPKFKENFWNDWRKLLGKNHV 420
Query: 421 IQNLDDCDFTPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF 480
IQNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF
Sbjct: 421 IQNLDDCDFTPIYDWYQMEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNF 480
Query: 481 RVEPPGLFRGRGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW 540
RVEPPGLFRGRGEHPKMGKLKRRI PSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW
Sbjct: 481 RVEPPGLFRGRGEHPKMGKLKRRIRPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTW 540
Query: 541 LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDST 600
LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKAR+LKDYIKNIRNAYTKDFTSKD T
Sbjct: 541 LAFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARVLKDYIKNIRNAYTKDFTSKDPT 600
Query: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKY 660
RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVK VPPNLLEFNFLGKDSI+Y
Sbjct: 601 RRQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIRY 660
Query: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASIT 720
ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDT+KLNAHLKDLMPGLTAKVFRTYNASIT
Sbjct: 661 ENTVEVELPVFKAITQFQSGKSGTDDLFDKLDTNKLNAHLKDLMPGLTAKVFRTYNASIT 720
Query: 721 LDDMLNRGTKDGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIV 780
LDDMLNRGTKDG+V EKI TYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI+
Sbjct: 721 LDDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIL 780
Query: 781 KELKIDLDRAKKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV 840
KELKIDLDRAKKGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV
Sbjct: 781 KELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTV 840
Query: 841 ALGTSKINYLDPRITVAWCKRHEVPIEK------------------IFNKSLLAKFAWAM 890
ALGTSKINYLDPRITVAWCKRHEVPIEK IFNKSLLAKFAWAM
Sbjct: 841 ALGTSKINYLDPRITVAWCKRHEVPIEKFKNFLLIFVIHNHNLLPQIFNKSLLAKFAWAM 900
BLAST of Bhi11G000412 vs. ExPASy TrEMBL
Match:
A0A6J1KZE0 (DNA topoisomerase I OS=Cucurbita maxima OX=3661 GN=LOC111500229 PE=3 SV=1)
HSP 1 Score: 1572.0 bits (4069), Expect = 0.0e+00
Identity = 814/889 (91.56%), Postives = 844/889 (94.94%), Query Frame = 0
Query: 1 MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRQGSDIC 60
MAVETFGKA MF DDDDEDNMPIVFKRSSASRQNQL+SE+KHSQSQRLN+LSGR GSD C
Sbjct: 1 MAVETFGKATMF-DDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDAC 60
Query: 61 STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSPIREPPVKPLGTSLKVSDSMDSRSKP 120
STNGQSSN QKRMTSLPKTSPMR EIGSQKASKS I+EP VK LGT+LKVSDSMDSR K
Sbjct: 61 STNGQSSNAQKRMTSLPKTSPMRPEIGSQKASKSSIQEPLVKSLGTTLKVSDSMDSRPKQ 120
Query: 121 LVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGNSSQPNKGLVPTVPKNKVKKSSGDSD 180
LVN AVK+EN SI QENSDSEDDKPLSIRLKGNS+ P+KG +PTV KNK+KKSS DSD
Sbjct: 121 LVNAAVKEENDSIHPAQENSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSD 180
Query: 181 DEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDKLQKPAVLSSKRPLPN 240
DE+PLSSKF+MKS SGVLG KQHGFDEKKPL +VHQNGSTS DKLQKPAV+SSKRPLPN
Sbjct: 181 DEVPLSSKFRMKSASGVLGGKQHGFDEKKPLDSKVHQNGSTSSDKLQKPAVVSSKRPLPN 240
Query: 241 ADSHTPSQISTKKPKLSDSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKSS 300
ADSH+ SQIS+KKPKLSDSSTPLS+ VSLKAKQEV D+DD IPISQRM KMSA VNKSS
Sbjct: 241 ADSHSSSQISSKKPKLSDSSTPLSTHTVSLKAKQEVVDDDDHIPISQRM-KMSAAVNKSS 300
Query: 301 SSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360
SK+TVSKV SSSIKKT KKSKKPVKKSKYVKSTKLLPS+GDGQKKWTTLVHNGVIFPPP
Sbjct: 301 GSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPP 360
Query: 361 YQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHVI 420
YQTHGVKIL+KGKPVDLTPEQEEVATMFAVMKDT+YM KPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKILHKGKPVDLTPEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420
Query: 421 QNLDDCDFTPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480
QNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR
Sbjct: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480
Query: 481 VEPPGLFRGRGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL 540
VEPPGLFRGRGEHPKMGKLK+RI PSDITINIGK APIPECPIPG+RWKEVRHDNTVTWL
Sbjct: 481 VEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWL 540
Query: 541 AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTR 600
AFWNDPIN KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRN+YTKDFTSKD TR
Sbjct: 541 AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTR 600
Query: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKYE 660
RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVK VPPNLLEFNFLGKDSIKYE
Sbjct: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE 660
Query: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720
NTVEVELPVFKAITQFQSGKSG+DDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL
Sbjct: 661 NTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720
Query: 721 DDMLNRGTKDGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVK 780
DDMLNRGTKDG+V EKI TYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI+K
Sbjct: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780
Query: 781 ELKIDLDRAKKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
ELKIDLDRAKKGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA
Sbjct: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
Query: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 890
LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887
BLAST of Bhi11G000412 vs. ExPASy TrEMBL
Match:
A0A6J1EWI0 (DNA topoisomerase I OS=Cucurbita moschata OX=3662 GN=LOC111438616 PE=3 SV=1)
HSP 1 Score: 1570.8 bits (4066), Expect = 0.0e+00
Identity = 813/889 (91.45%), Postives = 843/889 (94.83%), Query Frame = 0
Query: 1 MAVETFGKAPMFDDDDDEDNMPIVFKRSSASRQNQLDSEIKHSQSQRLNKLSGRQGSDIC 60
MAVETFGKAPMF DDDDEDNMPIVFKRSSASRQNQL+SE+KHSQSQRLN+LSGR GSD C
Sbjct: 1 MAVETFGKAPMF-DDDDEDNMPIVFKRSSASRQNQLNSEVKHSQSQRLNQLSGRPGSDAC 60
Query: 61 STNGQSSNNQKRMTSLPKTSPMRSEIGSQKASKSPIREPPVKPLGTSLKVSDSMDSRSKP 120
S NGQSSN QKRMTSLPKTSPMR EIGSQKA+KS I+EP VK LGT+LKVSDSMDSR K
Sbjct: 61 SANGQSSNAQKRMTSLPKTSPMRPEIGSQKAAKSSIQEPLVKSLGTTLKVSDSMDSRPKQ 120
Query: 121 LVNTAVKKENVSIILTQENSDSEDDKPLSIRLKGNSSQPNKGLVPTVPKNKVKKSSGDSD 180
L N AVK+EN SI TQENSDSEDDKPLSIRLKGNS+ P+KG +PTV KNK+KKSS DSD
Sbjct: 121 LANAAVKEENDSIHPTQENSDSEDDKPLSIRLKGNSNHPSKGPIPTVQKNKIKKSSEDSD 180
Query: 181 DEIPLSSKFQMKSGSGVLGSKQHGFDEKKPLVPRVHQNGSTSRDKLQKPAVLSSKRPLPN 240
DE+PLSSKFQMKS SGVLG KQHGF EKKPL +VHQNGSTSRDKLQKPAV+SSKRPLPN
Sbjct: 181 DEVPLSSKFQMKSASGVLGGKQHGFHEKKPLDSKVHQNGSTSRDKLQKPAVVSSKRPLPN 240
Query: 241 ADSHTPSQISTKKPKLSDSSTPLSSKLVSLKAKQEVDDEDDDIPISQRMKKMSAPVNKSS 300
ADSH+ SQIS+KKPKLSDSSTPLS+ VSLKAKQEV D+DD IPISQRM KMSA VNKSS
Sbjct: 241 ADSHSSSQISSKKPKLSDSSTPLSTNTVSLKAKQEVVDDDDHIPISQRM-KMSAAVNKSS 300
Query: 301 SSKQTVSKVVSSSIKKTFKKSKKPVKKSKYVKSTKLLPSAGDGQKKWTTLVHNGVIFPPP 360
SK+TVSKV SSSIKKT KKSKKPVKKSKYVKSTKLLPS+GDGQKKWTTLVHNGVIFPPP
Sbjct: 301 GSKKTVSKVASSSIKKTLKKSKKPVKKSKYVKSTKLLPSSGDGQKKWTTLVHNGVIFPPP 360
Query: 361 YQTHGVKILYKGKPVDLTPEQEEVATMFAVMKDTEYMLKPKFKENFWNDWRKLLGKNHVI 420
YQTHGVKIL+KGKPVDLT EQEEVATMFAVMKDT+YM KPKFKENFWNDWRKLLGKNHVI
Sbjct: 361 YQTHGVKILHKGKPVDLTTEQEEVATMFAVMKDTDYMQKPKFKENFWNDWRKLLGKNHVI 420
Query: 421 QNLDDCDFTPIYDWYQKEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480
QNLDDCDFTPIYDWYQ EKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR
Sbjct: 421 QNLDDCDFTPIYDWYQTEKEKKKQMTTEEKKALKEEKLKQEEKYMWAILDGVKEKVGNFR 480
Query: 481 VEPPGLFRGRGEHPKMGKLKRRIHPSDITINIGKGAPIPECPIPGERWKEVRHDNTVTWL 540
VEPPGLFRGRGEHPKMGKLK+RI PSDITINIGK APIPECPIPG+RWKEVRHDNTVTWL
Sbjct: 481 VEPPGLFRGRGEHPKMGKLKKRIRPSDITINIGKDAPIPECPIPGQRWKEVRHDNTVTWL 540
Query: 541 AFWNDPINQKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNAYTKDFTSKDSTR 600
AFWNDPIN KEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRN+YTKDFTSKD TR
Sbjct: 541 AFWNDPINPKEFKYVFLAASSSLKGQSDKEKYEKARMLKDYIKNIRNSYTKDFTSKDPTR 600
Query: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKVVPPNLLEFNFLGKDSIKYE 660
RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVK VPPNLLEFNFLGKDSIKYE
Sbjct: 601 RQIAVATYLIDKLALRAGNEKDDDEADTVGCCTLKVENVKAVPPNLLEFNFLGKDSIKYE 660
Query: 661 NTVEVELPVFKAITQFQSGKSGTDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720
NTVEVELPVFKAITQFQSGKSG+DDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL
Sbjct: 661 NTVEVELPVFKAITQFQSGKSGSDDLFDKLDTSKLNAHLKDLMPGLTAKVFRTYNASITL 720
Query: 721 DDMLNRGTKDGDVVEKIATYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDIVK 780
DDMLNRGTKDG+V EKI TYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDI+K
Sbjct: 721 DDMLNRGTKDGEVSEKIVTYQHANKEVAIICNHQRTVSKSHGAQMTRLNEKISELRDILK 780
Query: 781 ELKIDLDRAKKGKPPLKDADGKRKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
ELKIDLDRAKKGKPPLK +DGK+KRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA
Sbjct: 781 ELKIDLDRAKKGKPPLKGSDGKQKRNLTPEALEKKISQTNAKIEKIERDMKTKEDLKTVA 840
Query: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 890
LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF
Sbjct: 841 LGTSKINYLDPRITVAWCKRHEVPIEKIFNKSLLAKFAWAMDVDPDFRF 887
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
P30181 | 1.1e-297 | 61.28 | DNA topoisomerase 1 alpha OS=Arabidopsis thaliana OX=3702 GN=TOP1A PE=1 SV=1 | [more] |
Q9FJ79 | 6.4e-293 | 60.89 | DNA topoisomerase 1 beta OS=Arabidopsis thaliana OX=3702 GN=TOP1B PE=1 SV=1 | [more] |
P93119 | 1.3e-242 | 60.91 | DNA topoisomerase 1 OS=Daucus carota OX=4039 GN=TOP1 PE=2 SV=1 | [more] |
Q54RC3 | 3.3e-148 | 40.25 | DNA topoisomerase 1 OS=Dictyostelium discoideum OX=44689 GN=top1 PE=3 SV=1 | [more] |
P07799 | 3.6e-147 | 40.31 | DNA topoisomerase 1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=28... | [more] |
Match Name | E-value | Identity | Description | |
XP_038904656.1 | 0.0e+00 | 100.00 | DNA topoisomerase 1 alpha-like isoform X1 [Benincasa hispida] >XP_038904657.1 DN... | [more] |
XP_008449103.1 | 0.0e+00 | 93.71 | PREDICTED: DNA topoisomerase 1-like [Cucumis melo] >XP_008449104.1 PREDICTED: DN... | [more] |
XP_004149585.1 | 0.0e+00 | 93.26 | DNA topoisomerase 1 alpha [Cucumis sativus] >XP_011650506.1 DNA topoisomerase 1 ... | [more] |
KAA0065816.1 | 0.0e+00 | 91.85 | DNA topoisomerase 1-like [Cucumis melo var. makuwa] >TYJ96555.1 DNA topoisomeras... | [more] |
XP_023553297.1 | 0.0e+00 | 91.68 | DNA topoisomerase 1 beta-like [Cucurbita pepo subsp. pepo] | [more] |
Match Name | E-value | Identity | Description | |
A0A1S3BM72 | 0.0e+00 | 93.71 | DNA topoisomerase I OS=Cucumis melo OX=3656 GN=LOC103491068 PE=3 SV=1 | [more] |
A0A0A0L2W2 | 0.0e+00 | 93.26 | DNA topoisomerase I OS=Cucumis sativus OX=3659 GN=Csa_3G063680 PE=3 SV=1 | [more] |
A0A5A7VHI9 | 0.0e+00 | 91.85 | DNA topoisomerase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold546G... | [more] |
A0A6J1KZE0 | 0.0e+00 | 91.56 | DNA topoisomerase I OS=Cucurbita maxima OX=3661 GN=LOC111500229 PE=3 SV=1 | [more] |
A0A6J1EWI0 | 0.0e+00 | 91.45 | DNA topoisomerase I OS=Cucurbita moschata OX=3662 GN=LOC111438616 PE=3 SV=1 | [more] |