Bhi11G000327 (gene) Wax gourd (B227) v1

Overview
NameBhi11G000327
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Description4-hydroxy-4-methyl-2-oxoglutarate aldolase
Locationchr11: 9710086 .. 9712603 (-)
RNA-Seq ExpressionBhi11G000327
SyntenyBhi11G000327
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAGAATTTTGTGAGTATTTATTTATTATCCCTTTCATTGTTAGAGCTGATACTAAATCAGGCCCTTGCCAATGGGCTGTTTAATCTTTGAAATCTCAAACCATAGACCCAGCATTGAAGCTCACAAATCCATTGGTTTTTGAAGCCCAAAGGCACCAGCGACGATCATCGCTGTCGGGGCACAACAACCAAAAACGCCCTGGTCTATTCCATCATCTTGATAAGCTTTTCGCCATTTCTGAAAATTCTACGCCATGGGGGTGGCTTAAATTGACTCAGCCGCCTGTTTGATTGTTCAATTGGATGGTTTCGGGCCTTAGAGCTTTCCACAGAAGACGAGGGCTTCAGCTTTCAATTGGGTGAGTTTTCTCCATCATTTTGTCTTCACTTCTTTCTTCTAATACTGTTTACATGAGTTTCAAATAGTTCTAAGATGTGTTGAGGACTTGAAAATCCAGTTTAGTTTGTCTTCCTCTGGAAAATCATCCTTGGAATTTTGTTTCATTCGTACAGTGAGAATTTAGCTGTGTGAGTGTTTGAAAATGGGGTCTTATTTCTTCCTCAATTGGCTGTTGGATGCTGTTTTTTTATGCCTTTATGCAAGGTTGTTTTTCATGGGAGGAGTATTTAAACCATCTTCCATCGAATTTGAGAAAATTTTGCCACTGTGAACATAGGGAAGATGACCCTGATTGGGCTTTGAAAATTTTAGTTTAACCCATGGTTTCACTTGTACCATCCTATACATTGATGCTGAAGCTTTTTCTTGTCTGTCAATCAAGTGTTCAATCACACCTAACTTTAATAAACTGTATGGACTTTAGATATGATTTATGGAACAAACTGAATTCCATTTATCTTTTTGTTTTGGTACCATATCACAGCACTATTAAACAAGACTAACTTCAGTTTATTAGATAGATTAAATCCTAGTTTGAGTCATAAACTTCCACAGTTATTTTGTGTTGGCCAATGAATTTTCAGATATCATATATCTTAGTCCCTCAACTTTCAATTGCTTTGTTGTAGTCTCTAAACTATGCATTGGAAACTATTTTAGTCCATATAATTAAATTTCTATTAATTACTTATCAAAACTCATATTTATTTGGAACCTGAACAGCAAACATCTAACCGAATATATGTAGCTTCAAACATTTGCCTTTACGAACATCAATTGGTAATGTGCTTCCAATAAGTTTCTATTAACTAATTTTTTTTAATATTAACCGTAGAAGATAAAAATAAAACAGCATATTTGAATGTCCAAAACCAAATTAGATTTAAGACTATTAGGATTTTAACATAACTTGAGAAAGTAAATAGAACTTGCATAATCTCATTAAAGTGGAAGCAAGTTGTTAAATAGTACATCCAAATACAGGGGAAGTAACAATGTAAAAGACTAACTAACTAACTAATTAGTAATTACATTTAGTATAATAAACTCTATTAAAACATTTGGAAATTCATTCTTTTAACAACAATAATGATTATTTTCATCCCCAATCTTCTCTTGCTTTGCTTCTAAAACTTTCATCTCTCCTTGAAAGAAGGAAATGTAATATTTACACTTTTCTGATATTTCTGTAAAAGTTACTTCAAAACTATATCTTACCTGTTGACATATCCTTGCCAGCAGTGGAAGGCATGGCAGCTCCAGCAACAGCAGATGTTTGTGATGCAAATGCAGCACATGTCACAAATGGTGATCTCCGAGTACTGCAACCGAAATTTGATATTTACGGGCAACGGCGAGCATTTTCGGGACAGATAGTGACAGTCAAGGTATTTGAAGACAATGTTTTGGTAAGACAGCTTCTTGAAACCAAAGGTGAGGGGAGGGTTCTTGTGATAGATGGGAATGGAAGTAGGAGATGTGCATTGTTGGGAGGTAATCTTGGGCAGTTATCTCATAACAATGGGTGGGCTGGGATTGTCGTAAATGGTTGTATAAGAGATGTCGATGAGATCAATAATTGTGATATTGGTGTGAGAGCATTAGGGACCAATCCATTGAAGTCAAACAAGAAAGGGATGGGAGAAAAGCATGTAGTTCTTGAAATTGGTGGCACTTTGATTCATGAAGGAGAGTGGCTGTATGCTGATAGTGATGGCATTTTAGTGTCCAAATTTGAGTTGTCTGTATGAATAGATAGCCTGATCGAGGAGCATAGGAAAACTCTCTATTGCTTGTTTTCAAGGCTTTCTTGGCTCTGGTTATTGAGTTCCTTCAAAGTGTATTGGATTAAGTTATGCTATCTCTTTCATTATATTCAATGTATTTGTGATTCTAAATGAAGGGGTGATTAGAAAGTCAATTGTTGGGTTCTTTGTTTTGGAAGCACTTATTAGAATGTTGTGTACGTTGTAGTTAAGATCTGAAAAGAAACCAAGGTCATGCATCTACTGTCTTTTGAGTTTATGAATTATTTTAAAATTTTTAAATAATGTTGTGATTTAATGTTGAAATTATGACTTGATTTTATTAAGAGGGAATTCTTACCCTATATTTGAG

mRNA sequence

AGAGAATTTTGTGAGTATTTATTTATTATCCCTTTCATTGTTAGAGCTGATACTAAATCAGGCCCTTGCCAATGGGCTGTTTAATCTTTGAAATCTCAAACCATAGACCCAGCATTGAAGCTCACAAATCCATTGGTTTTTGAAGCCCAAAGGCACCAGCGACGATCATCGCTGTCGGGGCACAACAACCAAAAACGCCCTGGTCTATTCCATCATCTTGATAAGCTTTTCGCCATTTCTGAAAATTCTACGCCATGGGGGTGGCTTAAATTGACTCAGCCGCCTGTTTGATTGTTCAATTGGATGGTTTCGGGCCTTAGAGCTTTCCACAGAAGACGAGGGCTTCAGCTTTCAATTGGCAGTGGAAGGCATGGCAGCTCCAGCAACAGCAGATGTTTGTGATGCAAATGCAGCACATGTCACAAATGGTGATCTCCGAGTACTGCAACCGAAATTTGATATTTACGGGCAACGGCGAGCATTTTCGGGACAGATAGTGACAGTCAAGGTATTTGAAGACAATGTTTTGGTAAGACAGCTTCTTGAAACCAAAGGTGAGGGGAGGGTTCTTGTGATAGATGGGAATGGAAGTAGGAGATGTGCATTGTTGGGAGGTAATCTTGGGCAGTTATCTCATAACAATGGGTGGGCTGGGATTGTCGTAAATGGTTGTATAAGAGATGTCGATGAGATCAATAATTGTGATATTGGTGTGAGAGCATTAGGGACCAATCCATTGAAGTCAAACAAGAAAGGGATGGGAGAAAAGCATGTAGTTCTTGAAATTGGTGGCACTTTGATTCATGAAGGAGAGTGGCTGTATGCTGATAGTGATGGCATTTTAGTGTCCAAATTTGAGTTGTCTGTATGAATAGATAGCCTGATCGAGGAGCATAGGAAAACTCTCTATTGCTTGTTTTCAAGGCTTTCTTGGCTCTGGTTATTGAGTTCCTTCAAAGTGTATTGGATTAAGTTATGCTATCTCTTTCATTATATTCAATGTATTTGTGATTCTAAATGAAGGGGTGATTAGAAAGTCAATTGTTGGGTTCTTTGTTTTGGAAGCACTTATTAGAATGTTGTGTACGTTGTAGTTAAGATCTGAAAAGAAACCAAGGTCATGCATCTACTGTCTTTTGAGTTTATGAATTATTTTAAAATTTTTAAATAATGTTGTGATTTAATGTTGAAATTATGACTTGATTTTATTAAGAGGGAATTCTTACCCTATATTTGAG

Coding sequence (CDS)

ATGGCAGCTCCAGCAACAGCAGATGTTTGTGATGCAAATGCAGCACATGTCACAAATGGTGATCTCCGAGTACTGCAACCGAAATTTGATATTTACGGGCAACGGCGAGCATTTTCGGGACAGATAGTGACAGTCAAGGTATTTGAAGACAATGTTTTGGTAAGACAGCTTCTTGAAACCAAAGGTGAGGGGAGGGTTCTTGTGATAGATGGGAATGGAAGTAGGAGATGTGCATTGTTGGGAGGTAATCTTGGGCAGTTATCTCATAACAATGGGTGGGCTGGGATTGTCGTAAATGGTTGTATAAGAGATGTCGATGAGATCAATAATTGTGATATTGGTGTGAGAGCATTAGGGACCAATCCATTGAAGTCAAACAAGAAAGGGATGGGAGAAAAGCATGTAGTTCTTGAAATTGGTGGCACTTTGATTCATGAAGGAGAGTGGCTGTATGCTGATAGTGATGGCATTTTAGTGTCCAAATTTGAGTTGTCTGTATGA

Protein sequence

MAAPATADVCDANAAHVTNGDLRVLQPKFDIYGQRRAFSGQIVTVKVFEDNVLVRQLLETKGEGRVLVIDGNGSRRCALLGGNLGQLSHNNGWAGIVVNGCIRDVDEINNCDIGVRALGTNPLKSNKKGMGEKHVVLEIGGTLIHEGEWLYADSDGILVSKFELSV
Homology
BLAST of Bhi11G000327 vs. TAIR 10
Match: AT5G56260.1 (Ribonuclease E inhibitor RraA/Dimethylmenaquinone methyltransferase )

HSP 1 Score: 265.4 bits (677), Expect = 3.1e-71
Identity = 128/166 (77.11%), Postives = 144/166 (86.75%), Query Frame = 0

Query: 1   MAAPATADVCDANAAHVTNGDLRVLQPKFDIYGQRRAFSGQIVTVKVFEDNVLVRQLLET 60
           MAA ATA+ CD+NA  ++NGDLR L P F IYGQRR FSG IVT+KVFEDNVLVR  LET
Sbjct: 1   MAAFATAEACDSNAELISNGDLRALHPIFKIYGQRRCFSGPIVTLKVFEDNVLVRNQLET 60

Query: 61  KGEGRVLVIDGNGSRRCALLGGNLGQLSHNNGWAGIVVNGCIRDVDEINNCDIGVRALGT 120
           KGEG VLVIDG GS RCAL+GGNLGQL+ NNGW+GIVVNGC+RDVDEIN+CD+GVRALG+
Sbjct: 61  KGEGGVLVIDGGGSMRCALVGGNLGQLAQNNGWSGIVVNGCVRDVDEINDCDVGVRALGS 120

Query: 121 NPLKSNKKGMGEKHVVLEIGGTLIHEGEWLYADSDGILVSKFELSV 167
           NPLKS KKG GEK+V + IGGTLI +GEWLYADSDGIL+SK ELSV
Sbjct: 121 NPLKSTKKGHGEKNVPVHIGGTLIRDGEWLYADSDGILISKTELSV 166

BLAST of Bhi11G000327 vs. TAIR 10
Match: AT5G16450.1 (Ribonuclease E inhibitor RraA/Dimethylmenaquinone methyltransferase )

HSP 1 Score: 232.6 bits (592), Expect = 2.2e-61
Identity = 111/166 (66.87%), Postives = 134/166 (80.72%), Query Frame = 0

Query: 1   MAAPATADVCDANAAHVTNGDLRVLQPKFDIYGQRRAFSGQIVTVKVFEDNVLVRQLLET 60
           MA   TA+VCDAN   + +G LR LQP F IYG+R+ FSG +VTVKVFEDN L+RQ +E 
Sbjct: 1   MAFVTTAEVCDANQELIRSGQLRALQPIFQIYGRRQIFSGPVVTVKVFEDNGLIRQFIEE 60

Query: 61  KGEGRVLVIDGNGSRRCALLGGNLGQLSHNNGWAGIVVNGCIRDVDEINNCDIGVRALGT 120
           KG GRVLV+DG GS+RCA+LGGN    + NNGWAGIVVNGCIRDVDEIN CDIGVRAL +
Sbjct: 61  KGNGRVLVVDGGGSQRCAILGGNPVVQAQNNGWAGIVVNGCIRDVDEINGCDIGVRALAS 120

Query: 121 NPLKSNKKGMGEKHVVLEIGGTLIHEGEWLYADSDGILVSKFELSV 167
           +P+K++KKG+GE+ V + I GT I +GEWLYAD+DGILVS+ ELSV
Sbjct: 121 HPIKASKKGLGEQRVPVNIAGTRICDGEWLYADTDGILVSQIELSV 166

BLAST of Bhi11G000327 vs. TAIR 10
Match: AT5G16450.2 (Ribonuclease E inhibitor RraA/Dimethylmenaquinone methyltransferase )

HSP 1 Score: 232.6 bits (592), Expect = 2.2e-61
Identity = 111/166 (66.87%), Postives = 134/166 (80.72%), Query Frame = 0

Query: 1   MAAPATADVCDANAAHVTNGDLRVLQPKFDIYGQRRAFSGQIVTVKVFEDNVLVRQLLET 60
           MA   TA+VCDAN   + +G LR LQP F IYG+R+ FSG +VTVKVFEDN L+RQ +E 
Sbjct: 1   MAFVTTAEVCDANQELIRSGQLRALQPIFQIYGRRQIFSGPVVTVKVFEDNGLIRQFIEE 60

Query: 61  KGEGRVLVIDGNGSRRCALLGGNLGQLSHNNGWAGIVVNGCIRDVDEINNCDIGVRALGT 120
           KG GRVLV+DG GS+RCA+LGGN    + NNGWAGIVVNGCIRDVDEIN CDIGVRAL +
Sbjct: 61  KGNGRVLVVDGGGSQRCAILGGNPVVQAQNNGWAGIVVNGCIRDVDEINGCDIGVRALAS 120

Query: 121 NPLKSNKKGMGEKHVVLEIGGTLIHEGEWLYADSDGILVSKFELSV 167
           +P+K++KKG+GE+ V + I GT I +GEWLYAD+DGILVS+ ELSV
Sbjct: 121 HPIKASKKGLGEQRVPVNIAGTRICDGEWLYADTDGILVSQIELSV 166

BLAST of Bhi11G000327 vs. TAIR 10
Match: AT3G02770.1 (Ribonuclease E inhibitor RraA/Dimethylmenaquinone methyltransferase )

HSP 1 Score: 231.9 bits (590), Expect = 3.8e-61
Identity = 111/166 (66.87%), Postives = 132/166 (79.52%), Query Frame = 0

Query: 1   MAAPATADVCDANAAHVTNGDLRVLQPKFDIYGQRRAFSGQIVTVKVFEDNVLVRQLLET 60
           MA   TA+VCDAN   + +G LR LQP F IYG+R+ FSG +VTVKVFEDN L+R  LE 
Sbjct: 1   MAFVTTAEVCDANQEMIRSGQLRALQPVFQIYGRRQIFSGPVVTVKVFEDNGLIRHFLEE 60

Query: 61  KGEGRVLVIDGNGSRRCALLGGNLGQLSHNNGWAGIVVNGCIRDVDEINNCDIGVRALGT 120
           KG GRVLV+DG GS RCA+LGGN    + NNGWAGI+VNGCIRDVDEIN CDIGVRAL +
Sbjct: 61  KGNGRVLVVDGGGSLRCAILGGNPVVQAQNNGWAGIIVNGCIRDVDEINGCDIGVRALAS 120

Query: 121 NPLKSNKKGMGEKHVVLEIGGTLIHEGEWLYADSDGILVSKFELSV 167
           +P+K++KKG+GE+ V L I GT I +GEWLYAD+DGILVS+ ELSV
Sbjct: 121 HPIKASKKGLGEQRVSLNIAGTRICDGEWLYADTDGILVSQIELSV 166

BLAST of Bhi11G000327 vs. ExPASy Swiss-Prot
Match: Q9FH13 (Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 3 OS=Arabidopsis thaliana OX=3702 GN=At5g56260 PE=1 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 4.4e-70
Identity = 128/166 (77.11%), Postives = 144/166 (86.75%), Query Frame = 0

Query: 1   MAAPATADVCDANAAHVTNGDLRVLQPKFDIYGQRRAFSGQIVTVKVFEDNVLVRQLLET 60
           MAA ATA+ CD+NA  ++NGDLR L P F IYGQRR FSG IVT+KVFEDNVLVR  LET
Sbjct: 1   MAAFATAEACDSNAELISNGDLRALHPIFKIYGQRRCFSGPIVTLKVFEDNVLVRNQLET 60

Query: 61  KGEGRVLVIDGNGSRRCALLGGNLGQLSHNNGWAGIVVNGCIRDVDEINNCDIGVRALGT 120
           KGEG VLVIDG GS RCAL+GGNLGQL+ NNGW+GIVVNGC+RDVDEIN+CD+GVRALG+
Sbjct: 61  KGEGGVLVIDGGGSMRCALVGGNLGQLAQNNGWSGIVVNGCVRDVDEINDCDVGVRALGS 120

Query: 121 NPLKSNKKGMGEKHVVLEIGGTLIHEGEWLYADSDGILVSKFELSV 167
           NPLKS KKG GEK+V + IGGTLI +GEWLYADSDGIL+SK ELSV
Sbjct: 121 NPLKSTKKGHGEKNVPVHIGGTLIRDGEWLYADSDGILISKTELSV 166

BLAST of Bhi11G000327 vs. ExPASy Swiss-Prot
Match: Q9FFE0 (Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 OS=Arabidopsis thaliana OX=3702 GN=At5g16450 PE=1 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 3.1e-60
Identity = 111/166 (66.87%), Postives = 134/166 (80.72%), Query Frame = 0

Query: 1   MAAPATADVCDANAAHVTNGDLRVLQPKFDIYGQRRAFSGQIVTVKVFEDNVLVRQLLET 60
           MA   TA+VCDAN   + +G LR LQP F IYG+R+ FSG +VTVKVFEDN L+RQ +E 
Sbjct: 1   MAFVTTAEVCDANQELIRSGQLRALQPIFQIYGRRQIFSGPVVTVKVFEDNGLIRQFIEE 60

Query: 61  KGEGRVLVIDGNGSRRCALLGGNLGQLSHNNGWAGIVVNGCIRDVDEINNCDIGVRALGT 120
           KG GRVLV+DG GS+RCA+LGGN    + NNGWAGIVVNGCIRDVDEIN CDIGVRAL +
Sbjct: 61  KGNGRVLVVDGGGSQRCAILGGNPVVQAQNNGWAGIVVNGCIRDVDEINGCDIGVRALAS 120

Query: 121 NPLKSNKKGMGEKHVVLEIGGTLIHEGEWLYADSDGILVSKFELSV 167
           +P+K++KKG+GE+ V + I GT I +GEWLYAD+DGILVS+ ELSV
Sbjct: 121 HPIKASKKGLGEQRVPVNIAGTRICDGEWLYADTDGILVSQIELSV 166

BLAST of Bhi11G000327 vs. ExPASy Swiss-Prot
Match: Q9M8R9 (Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 1 OS=Arabidopsis thaliana OX=3702 GN=At3g02770 PE=2 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 5.3e-60
Identity = 111/166 (66.87%), Postives = 132/166 (79.52%), Query Frame = 0

Query: 1   MAAPATADVCDANAAHVTNGDLRVLQPKFDIYGQRRAFSGQIVTVKVFEDNVLVRQLLET 60
           MA   TA+VCDAN   + +G LR LQP F IYG+R+ FSG +VTVKVFEDN L+R  LE 
Sbjct: 1   MAFVTTAEVCDANQEMIRSGQLRALQPVFQIYGRRQIFSGPVVTVKVFEDNGLIRHFLEE 60

Query: 61  KGEGRVLVIDGNGSRRCALLGGNLGQLSHNNGWAGIVVNGCIRDVDEINNCDIGVRALGT 120
           KG GRVLV+DG GS RCA+LGGN    + NNGWAGI+VNGCIRDVDEIN CDIGVRAL +
Sbjct: 61  KGNGRVLVVDGGGSLRCAILGGNPVVQAQNNGWAGIIVNGCIRDVDEINGCDIGVRALAS 120

Query: 121 NPLKSNKKGMGEKHVVLEIGGTLIHEGEWLYADSDGILVSKFELSV 167
           +P+K++KKG+GE+ V L I GT I +GEWLYAD+DGILVS+ ELSV
Sbjct: 121 HPIKASKKGLGEQRVSLNIAGTRICDGEWLYADTDGILVSQIELSV 166

BLAST of Bhi11G000327 vs. ExPASy Swiss-Prot
Match: Q46ZV6 (Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cupriavidus pinatubonensis (strain JMP 134 / LMG 1197) OX=264198 GN=Reut_A1963 PE=3 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 1.5e-49
Identity = 85/159 (53.46%), Postives = 120/159 (75.47%), Query Frame = 0

Query: 6   TADVCDANAAHVTNGDLRVLQPKFDIYGQRRAFSGQIVTVKVFEDNVLVRQLLETKGEGR 65
           T D+CDAN   + +G LRV+ P F  +G+++AF+G   T+K+FEDN LVR+ LE+ G+GR
Sbjct: 5   TTDLCDANEPRLADGTLRVMAPVFRAFGKQQAFAGPAATLKIFEDNSLVREALESPGQGR 64

Query: 66  VLVIDGNGSRRCALLGGNLGQLSHNNGWAGIVVNGCIRDVDEINNCDIGVRALGTNPLKS 125
           VLV+DG GS RCAL+GGNLG L+  NGW GI+VNGC+RD  E++ CDIG+RAL  +P KS
Sbjct: 65  VLVVDGGGSLRCALVGGNLGLLAEKNGWVGIIVNGCVRDTGELDVCDIGIRALAAHPQKS 124

Query: 126 NKKGMGEKHVVLEIGGTLIHEGEWLYADSDGILVSKFEL 165
            K+G GE+ V +++ G ++  G W+Y D+DG+LVS+ +L
Sbjct: 125 QKRGAGERDVAVQMPGAVVRPGNWIYVDADGVLVSEEKL 163

BLAST of Bhi11G000327 vs. ExPASy Swiss-Prot
Match: B2JIG2 (Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Paraburkholderia phymatum (strain DSM 17167 / CIP 108236 / LMG 21445 / STM815) OX=391038 GN=Bphy_1374 PE=3 SV=1)

HSP 1 Score: 195.7 bits (496), Expect = 4.2e-49
Identity = 92/160 (57.50%), Postives = 117/160 (73.12%), Query Frame = 0

Query: 5   ATADVCDANAAHVTNGDLRVLQPKFDIYGQRRAFSGQIVTVKVFEDNVLVRQLLETKGEG 64
           ATAD+CDA+   +T G LRVL P F  +G+   F G+ VT+KVFEDN LVR  LE KG  
Sbjct: 4   ATADLCDAHEDQLTAGTLRVLDPVFSRFGRASRFGGEAVTLKVFEDNTLVRATLEEKGVA 63

Query: 65  RVLVIDGNGSRRCALLGGNLGQLSHNNGWAGIVVNGCIRDVDEINNCDIGVRALGTNPLK 124
           RVLV+DG GS RCAL+GGNLG+L   NGWAGIVV GC+RD  E+N C++G+ AL T+P +
Sbjct: 64  RVLVVDGGGSLRCALVGGNLGKLGEKNGWAGIVVFGCVRDTLELNECNLGIIALATHPQR 123

Query: 125 SNKKGMGEKHVVLEIGGTLIHEGEWLYADSDGILVSKFEL 165
           S K+G GE+ V L + G+++  GEW+YADSDG+LVS   L
Sbjct: 124 SQKRGGGERDVSLRLPGSVVRPGEWIYADSDGVLVSSAPL 163

BLAST of Bhi11G000327 vs. NCBI nr
Match: XP_038903077.1 (putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 3 [Benincasa hispida] >XP_038903078.1 putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 3 [Benincasa hispida])

HSP 1 Score: 339.3 bits (869), Expect = 1.8e-89
Identity = 166/166 (100.00%), Postives = 166/166 (100.00%), Query Frame = 0

Query: 1   MAAPATADVCDANAAHVTNGDLRVLQPKFDIYGQRRAFSGQIVTVKVFEDNVLVRQLLET 60
           MAAPATADVCDANAAHVTNGDLRVLQPKFDIYGQRRAFSGQIVTVKVFEDNVLVRQLLET
Sbjct: 1   MAAPATADVCDANAAHVTNGDLRVLQPKFDIYGQRRAFSGQIVTVKVFEDNVLVRQLLET 60

Query: 61  KGEGRVLVIDGNGSRRCALLGGNLGQLSHNNGWAGIVVNGCIRDVDEINNCDIGVRALGT 120
           KGEGRVLVIDGNGSRRCALLGGNLGQLSHNNGWAGIVVNGCIRDVDEINNCDIGVRALGT
Sbjct: 61  KGEGRVLVIDGNGSRRCALLGGNLGQLSHNNGWAGIVVNGCIRDVDEINNCDIGVRALGT 120

Query: 121 NPLKSNKKGMGEKHVVLEIGGTLIHEGEWLYADSDGILVSKFELSV 167
           NPLKSNKKGMGEKHVVLEIGGTLIHEGEWLYADSDGILVSKFELSV
Sbjct: 121 NPLKSNKKGMGEKHVVLEIGGTLIHEGEWLYADSDGILVSKFELSV 166

BLAST of Bhi11G000327 vs. NCBI nr
Match: XP_004148273.1 (putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 3 [Cucumis sativus] >XP_004148274.1 putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 3 [Cucumis sativus] >XP_011650460.1 putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 3 [Cucumis sativus] >XP_031739443.1 putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 3 [Cucumis sativus] >KGN55987.1 hypothetical protein Csa_009602 [Cucumis sativus])

HSP 1 Score: 330.1 bits (845), Expect = 1.1e-86
Identity = 160/166 (96.39%), Postives = 164/166 (98.80%), Query Frame = 0

Query: 1   MAAPATADVCDANAAHVTNGDLRVLQPKFDIYGQRRAFSGQIVTVKVFEDNVLVRQLLET 60
           MAAPATADVCDANAAHVTNGDLRVLQPKF+IYGQRRAFSGQIVTVKVFEDNVLVRQLLET
Sbjct: 1   MAAPATADVCDANAAHVTNGDLRVLQPKFEIYGQRRAFSGQIVTVKVFEDNVLVRQLLET 60

Query: 61  KGEGRVLVIDGNGSRRCALLGGNLGQLSHNNGWAGIVVNGCIRDVDEINNCDIGVRALGT 120
           KGEGRVLVIDGNGSRRCALLGGNLGQL+ NNGWAGIVVNGCIRDVDEINNCD+GVRALG 
Sbjct: 61  KGEGRVLVIDGNGSRRCALLGGNLGQLAQNNGWAGIVVNGCIRDVDEINNCDVGVRALGA 120

Query: 121 NPLKSNKKGMGEKHVVLEIGGTLIHEGEWLYADSDGILVSKFELSV 167
           NPLKSNKKGMGEKHVVL+IGGTLIHEGEWLYADSDGILVSKFELSV
Sbjct: 121 NPLKSNKKGMGEKHVVLQIGGTLIHEGEWLYADSDGILVSKFELSV 166

BLAST of Bhi11G000327 vs. NCBI nr
Match: XP_008448991.1 (PREDICTED: putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 3 [Cucumis melo] >XP_008448992.1 PREDICTED: putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 3 [Cucumis melo] >TYK19391.1 putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 3 [Cucumis melo var. makuwa])

HSP 1 Score: 329.7 bits (844), Expect = 1.4e-86
Identity = 160/166 (96.39%), Postives = 164/166 (98.80%), Query Frame = 0

Query: 1   MAAPATADVCDANAAHVTNGDLRVLQPKFDIYGQRRAFSGQIVTVKVFEDNVLVRQLLET 60
           MAAPATADVCDANAAHVTNGDLRVLQPKF+IYGQRRAFSGQIVTVKVFEDNVLVRQLLET
Sbjct: 1   MAAPATADVCDANAAHVTNGDLRVLQPKFEIYGQRRAFSGQIVTVKVFEDNVLVRQLLET 60

Query: 61  KGEGRVLVIDGNGSRRCALLGGNLGQLSHNNGWAGIVVNGCIRDVDEINNCDIGVRALGT 120
           KGEGRVLVIDGNGSRRCALLGGNLGQL+ NNGWAGIVVNGCIRDVDEINNCDIGVRALGT
Sbjct: 61  KGEGRVLVIDGNGSRRCALLGGNLGQLAQNNGWAGIVVNGCIRDVDEINNCDIGVRALGT 120

Query: 121 NPLKSNKKGMGEKHVVLEIGGTLIHEGEWLYADSDGILVSKFELSV 167
           NPLKSNKKGMG+KHVVL+IGGTLIHEGEWLYAD DGILVSKFELSV
Sbjct: 121 NPLKSNKKGMGDKHVVLQIGGTLIHEGEWLYADGDGILVSKFELSV 166

BLAST of Bhi11G000327 vs. NCBI nr
Match: XP_023540534.1 (putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 3 [Cucurbita pepo subsp. pepo] >XP_023540535.1 putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 3 [Cucurbita pepo subsp. pepo] >KAG7028314.1 putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 308.1 bits (788), Expect = 4.5e-80
Identity = 148/166 (89.16%), Postives = 159/166 (95.78%), Query Frame = 0

Query: 1   MAAPATADVCDANAAHVTNGDLRVLQPKFDIYGQRRAFSGQIVTVKVFEDNVLVRQLLET 60
           MAAPATAD+CDANAAHVT+GDLRVLQ KF IYGQRRAFSG IVTVKVFEDNVLVRQLLE+
Sbjct: 1   MAAPATADICDANAAHVTSGDLRVLQQKFKIYGQRRAFSGPIVTVKVFEDNVLVRQLLES 60

Query: 61  KGEGRVLVIDGNGSRRCALLGGNLGQLSHNNGWAGIVVNGCIRDVDEINNCDIGVRALGT 120
           +GEGRVLVIDGNGSRRCALLGGNLGQ++ + GWAG+VVNGCIRDVDEINNCD+GVRALGT
Sbjct: 61  QGEGRVLVIDGNGSRRCALLGGNLGQVAQSMGWAGVVVNGCIRDVDEINNCDVGVRALGT 120

Query: 121 NPLKSNKKGMGEKHVVLEIGGTLIHEGEWLYADSDGILVSKFELSV 167
           NPLKSNKKG+GEKHV + IGGTLIHEGEWLYADSDGILVSKFELSV
Sbjct: 121 NPLKSNKKGLGEKHVPVHIGGTLIHEGEWLYADSDGILVSKFELSV 166

BLAST of Bhi11G000327 vs. NCBI nr
Match: KAG6596786.1 (putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 308.1 bits (788), Expect = 4.5e-80
Identity = 148/166 (89.16%), Postives = 159/166 (95.78%), Query Frame = 0

Query: 1   MAAPATADVCDANAAHVTNGDLRVLQPKFDIYGQRRAFSGQIVTVKVFEDNVLVRQLLET 60
           MAAPATAD+CDANAAHVT+GDLRVLQ KF IYGQRRAFSG IVTVKVFEDNVLVRQLLE+
Sbjct: 14  MAAPATADICDANAAHVTSGDLRVLQQKFKIYGQRRAFSGPIVTVKVFEDNVLVRQLLES 73

Query: 61  KGEGRVLVIDGNGSRRCALLGGNLGQLSHNNGWAGIVVNGCIRDVDEINNCDIGVRALGT 120
           +GEGRVLVIDGNGSRRCALLGGNLGQ++ + GWAG+VVNGCIRDVDEINNCD+GVRALGT
Sbjct: 74  QGEGRVLVIDGNGSRRCALLGGNLGQVAQSMGWAGVVVNGCIRDVDEINNCDVGVRALGT 133

Query: 121 NPLKSNKKGMGEKHVVLEIGGTLIHEGEWLYADSDGILVSKFELSV 167
           NPLKSNKKG+GEKHV + IGGTLIHEGEWLYADSDGILVSKFELSV
Sbjct: 134 NPLKSNKKGLGEKHVPVHIGGTLIHEGEWLYADSDGILVSKFELSV 179

BLAST of Bhi11G000327 vs. ExPASy TrEMBL
Match: A0A0A0L5X9 (4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cucumis sativus OX=3659 GN=Csa_3G044520 PE=3 SV=1)

HSP 1 Score: 330.1 bits (845), Expect = 5.3e-87
Identity = 160/166 (96.39%), Postives = 164/166 (98.80%), Query Frame = 0

Query: 1   MAAPATADVCDANAAHVTNGDLRVLQPKFDIYGQRRAFSGQIVTVKVFEDNVLVRQLLET 60
           MAAPATADVCDANAAHVTNGDLRVLQPKF+IYGQRRAFSGQIVTVKVFEDNVLVRQLLET
Sbjct: 1   MAAPATADVCDANAAHVTNGDLRVLQPKFEIYGQRRAFSGQIVTVKVFEDNVLVRQLLET 60

Query: 61  KGEGRVLVIDGNGSRRCALLGGNLGQLSHNNGWAGIVVNGCIRDVDEINNCDIGVRALGT 120
           KGEGRVLVIDGNGSRRCALLGGNLGQL+ NNGWAGIVVNGCIRDVDEINNCD+GVRALG 
Sbjct: 61  KGEGRVLVIDGNGSRRCALLGGNLGQLAQNNGWAGIVVNGCIRDVDEINNCDVGVRALGA 120

Query: 121 NPLKSNKKGMGEKHVVLEIGGTLIHEGEWLYADSDGILVSKFELSV 167
           NPLKSNKKGMGEKHVVL+IGGTLIHEGEWLYADSDGILVSKFELSV
Sbjct: 121 NPLKSNKKGMGEKHVVLQIGGTLIHEGEWLYADSDGILVSKFELSV 166

BLAST of Bhi11G000327 vs. ExPASy TrEMBL
Match: A0A1S3BKF1 (4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cucumis melo OX=3656 GN=LOC103490998 PE=3 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 7.0e-87
Identity = 160/166 (96.39%), Postives = 164/166 (98.80%), Query Frame = 0

Query: 1   MAAPATADVCDANAAHVTNGDLRVLQPKFDIYGQRRAFSGQIVTVKVFEDNVLVRQLLET 60
           MAAPATADVCDANAAHVTNGDLRVLQPKF+IYGQRRAFSGQIVTVKVFEDNVLVRQLLET
Sbjct: 1   MAAPATADVCDANAAHVTNGDLRVLQPKFEIYGQRRAFSGQIVTVKVFEDNVLVRQLLET 60

Query: 61  KGEGRVLVIDGNGSRRCALLGGNLGQLSHNNGWAGIVVNGCIRDVDEINNCDIGVRALGT 120
           KGEGRVLVIDGNGSRRCALLGGNLGQL+ NNGWAGIVVNGCIRDVDEINNCDIGVRALGT
Sbjct: 61  KGEGRVLVIDGNGSRRCALLGGNLGQLAQNNGWAGIVVNGCIRDVDEINNCDIGVRALGT 120

Query: 121 NPLKSNKKGMGEKHVVLEIGGTLIHEGEWLYADSDGILVSKFELSV 167
           NPLKSNKKGMG+KHVVL+IGGTLIHEGEWLYAD DGILVSKFELSV
Sbjct: 121 NPLKSNKKGMGDKHVVLQIGGTLIHEGEWLYADGDGILVSKFELSV 166

BLAST of Bhi11G000327 vs. ExPASy TrEMBL
Match: A0A5D3D743 (4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G00240 PE=3 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 7.0e-87
Identity = 160/166 (96.39%), Postives = 164/166 (98.80%), Query Frame = 0

Query: 1   MAAPATADVCDANAAHVTNGDLRVLQPKFDIYGQRRAFSGQIVTVKVFEDNVLVRQLLET 60
           MAAPATADVCDANAAHVTNGDLRVLQPKF+IYGQRRAFSGQIVTVKVFEDNVLVRQLLET
Sbjct: 1   MAAPATADVCDANAAHVTNGDLRVLQPKFEIYGQRRAFSGQIVTVKVFEDNVLVRQLLET 60

Query: 61  KGEGRVLVIDGNGSRRCALLGGNLGQLSHNNGWAGIVVNGCIRDVDEINNCDIGVRALGT 120
           KGEGRVLVIDGNGSRRCALLGGNLGQL+ NNGWAGIVVNGCIRDVDEINNCDIGVRALGT
Sbjct: 61  KGEGRVLVIDGNGSRRCALLGGNLGQLAQNNGWAGIVVNGCIRDVDEINNCDIGVRALGT 120

Query: 121 NPLKSNKKGMGEKHVVLEIGGTLIHEGEWLYADSDGILVSKFELSV 167
           NPLKSNKKGMG+KHVVL+IGGTLIHEGEWLYAD DGILVSKFELSV
Sbjct: 121 NPLKSNKKGMGDKHVVLQIGGTLIHEGEWLYADGDGILVSKFELSV 166

BLAST of Bhi11G000327 vs. ExPASy TrEMBL
Match: A0A6J1GE55 (4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cucurbita moschata OX=3662 GN=LOC111453129 PE=3 SV=1)

HSP 1 Score: 307.8 bits (787), Expect = 2.8e-80
Identity = 147/166 (88.55%), Postives = 159/166 (95.78%), Query Frame = 0

Query: 1   MAAPATADVCDANAAHVTNGDLRVLQPKFDIYGQRRAFSGQIVTVKVFEDNVLVRQLLET 60
           MAAPATAD+CDANAAHVT+GDLRVLQ KF +YGQRRAFSG IVTVKVFEDNVLVRQLLE+
Sbjct: 1   MAAPATADICDANAAHVTSGDLRVLQQKFKVYGQRRAFSGPIVTVKVFEDNVLVRQLLES 60

Query: 61  KGEGRVLVIDGNGSRRCALLGGNLGQLSHNNGWAGIVVNGCIRDVDEINNCDIGVRALGT 120
           +GEGRVLVIDGNGSRRCALLGGNLGQ++ + GWAG+VVNGCIRDVDEINNCD+GVRALGT
Sbjct: 61  QGEGRVLVIDGNGSRRCALLGGNLGQVAQSMGWAGVVVNGCIRDVDEINNCDVGVRALGT 120

Query: 121 NPLKSNKKGMGEKHVVLEIGGTLIHEGEWLYADSDGILVSKFELSV 167
           NPLKSNKKG+GEKHV + IGGTLIHEGEWLYADSDGILVSKFELSV
Sbjct: 121 NPLKSNKKGLGEKHVPVHIGGTLIHEGEWLYADSDGILVSKFELSV 166

BLAST of Bhi11G000327 vs. ExPASy TrEMBL
Match: A0A6J1KXS9 (4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cucurbita maxima OX=3661 GN=LOC111498522 PE=3 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 1.1e-79
Identity = 147/166 (88.55%), Postives = 158/166 (95.18%), Query Frame = 0

Query: 1   MAAPATADVCDANAAHVTNGDLRVLQPKFDIYGQRRAFSGQIVTVKVFEDNVLVRQLLET 60
           MAAPATAD+CDANAAHV +GDLRVLQ KF IYGQRRAFSG IVTVKVFEDNVLVRQLLE+
Sbjct: 1   MAAPATADICDANAAHVISGDLRVLQQKFKIYGQRRAFSGPIVTVKVFEDNVLVRQLLES 60

Query: 61  KGEGRVLVIDGNGSRRCALLGGNLGQLSHNNGWAGIVVNGCIRDVDEINNCDIGVRALGT 120
           +GEGRVLVIDGNGSRRCALLGGNLGQ++ + GWAG+VVNGCIRDVDEINNCD+GVRALGT
Sbjct: 61  QGEGRVLVIDGNGSRRCALLGGNLGQVAQSMGWAGVVVNGCIRDVDEINNCDVGVRALGT 120

Query: 121 NPLKSNKKGMGEKHVVLEIGGTLIHEGEWLYADSDGILVSKFELSV 167
           NPLKSNKKG+GEKHV + IGGTLIHEGEWLYADSDGILVSKFELSV
Sbjct: 121 NPLKSNKKGLGEKHVPVHIGGTLIHEGEWLYADSDGILVSKFELSV 166

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G56260.13.1e-7177.11Ribonuclease E inhibitor RraA/Dimethylmenaquinone methyltransferase [more]
AT5G16450.12.2e-6166.87Ribonuclease E inhibitor RraA/Dimethylmenaquinone methyltransferase [more]
AT5G16450.22.2e-6166.87Ribonuclease E inhibitor RraA/Dimethylmenaquinone methyltransferase [more]
AT3G02770.13.8e-6166.87Ribonuclease E inhibitor RraA/Dimethylmenaquinone methyltransferase [more]
Match NameE-valueIdentityDescription
Q9FH134.4e-7077.11Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 3 OS=Arabidopsis thaliana OX... [more]
Q9FFE03.1e-6066.87Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 2 OS=Arabidopsis thaliana OX... [more]
Q9M8R95.3e-6066.87Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 1 OS=Arabidopsis thaliana OX... [more]
Q46ZV61.5e-4953.46Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cupriavidus pinatubonensi... [more]
B2JIG24.2e-4957.50Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Paraburkholderia phymatum... [more]
Match NameE-valueIdentityDescription
XP_038903077.11.8e-89100.00putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 3 [Benincasa hispida] >XP_03... [more]
XP_004148273.11.1e-8696.39putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 3 [Cucumis sativus] >XP_0041... [more]
XP_008448991.11.4e-8696.39PREDICTED: putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 3 [Cucumis melo] ... [more]
XP_023540534.14.5e-8089.16putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 3 [Cucurbita pepo subsp. pep... [more]
KAG6596786.14.5e-8089.16putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase 3, partial [Cucurbita argyro... [more]
Match NameE-valueIdentityDescription
A0A0A0L5X95.3e-8796.394-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cucumis sativus OX=3659 GN=Csa_3G0... [more]
A0A1S3BKF17.0e-8796.394-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cucumis melo OX=3656 GN=LOC1034909... [more]
A0A5D3D7437.0e-8796.394-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cucumis melo var. makuwa OX=119469... [more]
A0A6J1GE552.8e-8088.554-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1KXS91.1e-7988.554-hydroxy-4-methyl-2-oxoglutarate aldolase OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.50.30.40coord: 2..166
e-value: 1.3E-49
score: 170.0
NoneNo IPR availablePANTHERPTHR33254:SF224-HYDROXY-4-METHYL-2-OXOGLUTARATE ALDOLASEcoord: 1..166
NoneNo IPR availablePANTHERPTHR332544-HYDROXY-4-METHYL-2-OXOGLUTARATE ALDOLASE 3-RELATEDcoord: 1..166
IPR005493Ribonuclease E inhibitor RraA/RraA-like proteinPFAMPF03737RraA-likecoord: 6..159
e-value: 6.5E-45
score: 152.9
IPR005493Ribonuclease E inhibitor RraA/RraA-like proteinCDDcd16841RraA_familycoord: 7..160
e-value: 4.74695E-57
score: 173.8
IPR010203Regulator of ribonuclease activity ATIGRFAMTIGR01935TIGR01935coord: 6..160
e-value: 3.6E-63
score: 209.9
IPR036704Ribonuclease E inhibitor RraA/RraA-like superfamilySUPERFAMILY89562RraA-likecoord: 5..164

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi11M000327Bhi11M000327mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0051252 regulation of RNA metabolic process
molecular_function GO:0047443 4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0008948 oxaloacetate decarboxylase activity
molecular_function GO:0008428 ribonuclease inhibitor activity