Bhi11G000062 (gene) Wax gourd (B227) v1

Overview
NameBhi11G000062
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptionvesicle-associated protein 2-1
Locationchr11: 1760472 .. 1765430 (+)
RNA-Seq ExpressionBhi11G000062
SyntenyBhi11G000062
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCTTTCCGACACGTGTCCCACTCTCTCTCCCTCTCCTCTCTCTCTCTCTAGTTTCAGTGAAGCTCTGCAGTTGCCATCGGAGAGGTTACCAAATAAGCTTTCGCTTATGCTTTCCTCCTTTTTCGCTCTCGATTTCCCCATCTTTCCTTCTCTTTCTCTTCACTATTCCTTTTTCTCTGCCTAATTTTTCGAAACCCCTTTTGTTTTTCTTTCGATTCAATTTCGAGTTGGACCGAGCAATCCGTACTCTTCCCCCCTTTCCCTTCGCTTCACCACTAATTCAATCTCTATCTTGTTCATATCCTCATCTTTTTTTCTATTTCCTTCTCTCTTTTTTAGGGTACTGGCATTCCGCTGTTTTTGGATTTTCTGGGTTCTTTTTTTTGTTCTGTTTCTGGGTTGTTTCTTTTAATGCTTGCTTTCTGGAGTTGAAATCGGTAACTGGGTCGATTTTTGTTTTGGGCTTTTCCATTTTTTATCTGGGTTTCTGCCGTCATGAGTGCTGGAGGTGGGAATCGCTTAATCTCCGTTCAACCGGATGAGCTTAAGTTTCAGAGTATGTAATTTTGCTTTGATGTTTCTGATATCTTGTTGTTTTTATCTACATTTGATTGAATTTGCCTTGATTAGATGAAGTTTTGTGATAGTTGTTGAGGATTAGGGTTGATGATGATTTCATAGGAGTTGAGAGAAACTCCCAATGCTGTGAGTTTGAGCATTTTGTAGGTCTTCCTTTGTTTATTATAGATAAGGATTGATGATTCATAGAAAGTTTTTTTTTTTTTTTTGGAACCTGCTAAATACTCAATAATTCAGTGAAACTTTCAATTTCCTCGCGTTGAGTATTGAGAATTATAGTATTTATACCTTTTACCTTCTAAAATTCAATTATATCCTAGCCCTTCCCCTGTAGTTACTGTTCATCTCGTCCACTTTCAGTCCCTCCTCTTTCTTTTTGTTTTCTCATTTTTCTTCTGCTCATTACATGAGGGACAAAGGCTCCAAACTCCAAGGTTTTTTCATGGAGGTGGAAGCCTTTTCTGTGAGAGGCATAAGCCTCTTGTGAACGAAAACAAGATGATAACATTTGTATGTAGACTTGAGGTTAGCATCCATGAGGATTTTAATCGATTGTTGGGTAGCATTATCATACTTTTGTGTTGAAAATTTTGGAGCTGGTTGATTGGGTCTAGAATAAGATTGGATACAGTTATCATGGCTCAGTGTTTGGTGTTTTGTTATTGAACTTAAATGATTACTCTGTTGTATCTCATATATTATGGTTGGGATTTAAAGCTATTTACTGGCTGCAGCTTAGTTCTTTTGATAAATGGAAATCTTTTATACCACTAGAAAATGTATACATTGTTTCTTTTTTTCTTTACCCTTAATCTCAAATGTCTTTTGGTATTATCCATTGCTTCTTCAATCCATGAAATCTTAGGAAAAAGAAGTAATATTACCAATGTATCGTATCAAAGATACAATAAAGGATGTTTATTCAGTGGTTTTCTTTGTTCGCAAGTTTTGTTGTATTCTTACATGTGGACTTTGTTACATCTGTTTTCTTGGTGGACTCTATTGCGTTTTGAACTTATGAACGTGGGGCCTATATCAACAGTTGAGTTTGAAAAGCAGAGCTTTTGTGATCTTAAAGTAGCAAACAACACGGAACATCATGTTGCCTTTAAGGTAATAGCTTGATTATTTTCAAAATGCATACATAATTGACAATGAATATCGATTTTGGAAAGCATTTTGCATAACTGGTTGACTTCAAACTTATTTCTTCATCGCTTATGCTAGGTCAAAACGACCTCACCCAAGAAATACTTTGTGCGTCCCAACACTGGTGTTGTTCAGCCTTGGGATTCTTGCATCATAAGAGGTTTGGCTTTTATATAAGCATTTTATGTTTATGCTTTTCAATGATCTTTAACTCATAATATCTTCAGAAAATTTGAGGATGCAATTGCAATTGTTTCAAGCTTGAAAGAAAAAGAATGTTGGTTCCTTTGTAATTGGAAAATCTTTAGAGGATTTTTTTTAATACCACATCATGTAAAACTAGTAAGTGGCCTTGACAAATTAAGACTTCCTTTGCACTAGCAGATGTTAACTGCTACTTGTCAGCTAACCTAAATATTATTTGCATTCAAGTTATCTCTCTTGATTCGTCTTGGTTTATTTTTATCTGTTAAACTAAAGTCAAAGTTGTTGCTGGATTCACAGTGACTCTCCAAGCCCAGCGAGAGTATCCTGCAGATATGCAATGCAAGGATAAGTTTCTCCTACAAAGTACAATAGTCCCACCAAATACTGATGTAGATGAGCTTCCTTCTGATGCTGTAAGAGTTTTGACTTGTCCCTTAGTATGAATGGACATACCGTTTCATTCTAATAAGCTTCAATCTAAACTTTATTTTCTTATTTCTAGTTCAATAAGGACAGTGGAAGGACAATTGAGGAATGCAAGCTTAAGGTTATCTATATTTCTCCTGCATCAGCCTTGGGGAACTCAGAAGATGAAAAGAACTCATCTCAAAGCTCCTCGGTGAGTGCTGAAATTCTCCCTTTTGTTCCTTGAATATATATATATATATATTGGATGTTCTCTTTTGTTCCCTGTATAACCATGTTATAGGGTAATCGTGTGCTTGCAATAGATGTGTATAGAGATAACAAATTCGAAATATCACTTTTATTCTATGCTCTCATCCTCCTATTATATATATGTTTTTAACATTTGTTGTCCAGTAGTTGTCTGCATGTTCGTAAGTTAAACCTCTATTGTTCGTAAATTTTAAACACACATAGATTGTTCTTCCTTATCTCATATACCGTTGGTACCAACACGAGCAGGCTGTACAACGTCTGAAGGAAGAAAGAGACATGGCTATAAGGCAAACCCATCAATTACAACAGGAACTGGTGAGTCACACCTCTCTGCTCTATTGTGCTTTTTTGCTGCTAAAAGACTTGTAATAACCAAGTCTTTTAACTTGGTCAATGCATAGTTCATATAATATTGAGTTGAATCAGAATGAAGGACCTAAAATTAAATTTGAAATGTGATTGAATCTGATTTTTGACTCATAATTTTCCTATGGATGAATAGTAGGATTGCACAAAAGTGTTAACGTCATTAATACAAGAATAAAATATCATACATGTTTCAAGTTACACAACAATTAATAGCATTGAAGTCTGAGAGAGAAGCTTTATATATGAATTTGGGCCAGATGGGGTCCAGAATGATCAATGGTAGAGGGTTTTTTAAAGGAAAAAAAAATGTTTAAGAAACCAAGCTTTCATGGAACAAAATAAAAGAATATAGAAGGGCATACAAAAAAAATCAACAAGAAGCCCAACAGAAGGGAGACCCAAAACACAACTTACAAGAATGGGCTTCAATCCAACAAAATGAAACCAAAATCATAGTTACAAAAAGGTAGTTTGTTAAATGGTAGGATTGCACATAAGCTTTGACATCAATGGTAGTCTAGTCAATTTCATAAAAATGGTAGTTTGTTAAATCCACTCACAACCATGAACAACAACTTTTCTTTTGGAGGGGAGTGGTTTGATCTATTTTGTTGTTTCCTATACATGTGGCATGTCAAATGCCAGAGAATTGCCCATAAACTTTTTCTTCTTAATGATAAACCAACCTTTTCATTGAAAAAAAATAAATAAAAGAATAGAAGGGCAAAAAAAGAAAAATAATAAAAAAAACCTACAGAAGGAAGGAGCTCCAATTAAGCCAAATAACACCCTAATGAATAACTACCAAAAAACTTCGTCACCGAAGCATATAGAACCTAACAAGGGACCAAACACTAGTAGATTCTCTCGCCCTTCCTCTAAATACTATATTGTTCCTCTCCCTCCAAAGATCTCACAACAAAACACACATTTGAGCTTACCACAAAAACCTCCCCTTAACTACTCAAACATCTAGCTGAAATCTCTGTGATGGGCTAACTGAAAGCCAAATGCTCCAAAAAAGTTGCTCCAAATGAACCGAGCAAAATCGTAAGAATATGATCTAGGCCTTCCTTTGCCCTTTGACAAAGAATTGCTTATAAACTTAGAACACGGCTGGCACAAATTCCATATTAGCTTGAAAGAATAATTGTTATCATTCATTTGACATGCACATTTCTGAGCTCAATATTTATTTGATTCTTAGTTCCAAGGGTTACCTATCCAATAGGATGTCAGTTTTCCTTTTCCTTTATACAATTGTTATATGGGAGTATCCCCACTCCTGGCACCTATGCTCATGGCCTTCCCCTGCTAATCCTCACAGAGGTTTATAAAAATTATGTTTTTAGCATTATTAGTAGCTGGTTGGTACTAAAAAAAATAAAAAAGAAAAAGAAAAAAAGGACCTTGTCATCGTCGTCTTTTACAAATGTAGAACATCGATGGCGATTTAATACTTTATACCAATTATTCCTCTCCGATAGGACATGATGAAGAGACGAAAATATCGGAAAGGCGATCCTGGCTTCTCGTTTACTTTCGCGATCTTCGTGGGGATCGTTGGGATCATGGCAGGTTTCCTCTTGAATCTCACATTATCTTCACCATCTACGGAATGACTTGGACCATGTTTCAGATCCTGAGCAGACAAATTTCTAGTCAATTTCATTGTATAATTTTTACTTGCCAATTTTTGTACATTAAGAAACGCTGCAAATTTGTGGTCTTGATACCTTAATTTTTCCAAAATGTTGATTTTTGTGCCAATTGACTGTGAATGTTGCTGGCGATCTCTTCTGAATCCTCCTTCAAAACTGAGGTTCAGAGAAGTTAAGTTTTAGTTCATATCTTTTATATAACTAAATTCTATGATGTTTCTTTTAAAATGTAATTTCTGTATGTAATATGAATTTGTTGAATACATATTCATCAAGATGGAGATTTGATCCTAAAATCTTTAGTGATTTGAG

mRNA sequence

CTCTTTCCGACACGTGTCCCACTCTCTCTCCCTCTCCTCTCTCTCTCTCTAGTTTCAGTGAAGCTCTGCAGTTGCCATCGGAGAGGTTACCAAATAAGCTTTCGCTTATGCTTTCCTCCTTTTTCGCTCTCGATTTCCCCATCTTTCCTTCTCTTTCTCTTCACTATTCCTTTTTCTCTGCCTAATTTTTCGAAACCCCTTTTGTTTTTCTTTCGATTCAATTTCGAGTTGGACCGAGCAATCCGTACTCTTCCCCCCTTTCCCTTCGCTTCACCACTAATTCAATCTCTATCTTGTTCATATCCTCATCTTTTTTTCTATTTCCTTCTCTCTTTTTTAGGGTACTGGCATTCCGCTGTTTTTGGATTTTCTGGGTTCTTTTTTTTGTTCTGTTTCTGGGTTGTTTCTTTTAATGCTTGCTTTCTGGAGTTGAAATCGGTAACTGGGTCGATTTTTGTTTTGGGCTTTTCCATTTTTTATCTGGGTTTCTGCCGTCATGAGTGCTGGAGGTGGGAATCGCTTAATCTCCGTTCAACCGGATGAGCTTAAGTTTCAGATTGAGTTTGAAAAGCAGAGCTTTTGTGATCTTAAAGTAGCAAACAACACGGAACATCATGTTGCCTTTAAGGTCAAAACGACCTCACCCAAGAAATACTTTGTGCGTCCCAACACTGGTGTTGTTCAGCCTTGGGATTCTTGCATCATAAGAGTGACTCTCCAAGCCCAGCGAGAGTATCCTGCAGATATGCAATGCAAGGATAAGTTTCTCCTACAAAGTACAATAGTCCCACCAAATACTGATGTAGATGAGCTTCCTTCTGATGCTTTCAATAAGGACAGTGGAAGGACAATTGAGGAATGCAAGCTTAAGGTTATCTATATTTCTCCTGCATCAGCCTTGGGGAACTCAGAAGATGAAAAGAACTCATCTCAAAGCTCCTCGGCTGTACAACGTCTGAAGGAAGAAAGAGACATGGCTATAAGGCAAACCCATCAATTACAACAGGAACTGGACATGATGAAGAGACGAAAATATCGGAAAGGCGATCCTGGCTTCTCGTTTACTTTCGCGATCTTCGTGGGGATCGTTGGGATCATGGCAGGTTTCCTCTTGAATCTCACATTATCTTCACCATCTACGGAATGACTTGGACCATGTTTCAGATCCTGAGCAGACAAATTTCTAGTCAATTTCATTGTATAATTTTTACTTGCCAATTTTTGTACATTAAGAAACGCTGCAAATTTGTGGTCTTGATACCTTAATTTTTCCAAAATGTTGATTTTTGTGCCAATTGACTGTGAATGTTGCTGGCGATCTCTTCTGAATCCTCCTTCAAAACTGAGGTTCAGAGAAGTTAAGTTTTAGTTCATATCTTTTATATAACTAAATTCTATGATGTTTCTTTTAAAATGTAATTTCTGTATGTAATATGAATTTGTTGAATACATATTCATCAAGATGGAGATTTGATCCTAAAATCTTTAGTGATTTGAG

Coding sequence (CDS)

ATGAGTGCTGGAGGTGGGAATCGCTTAATCTCCGTTCAACCGGATGAGCTTAAGTTTCAGATTGAGTTTGAAAAGCAGAGCTTTTGTGATCTTAAAGTAGCAAACAACACGGAACATCATGTTGCCTTTAAGGTCAAAACGACCTCACCCAAGAAATACTTTGTGCGTCCCAACACTGGTGTTGTTCAGCCTTGGGATTCTTGCATCATAAGAGTGACTCTCCAAGCCCAGCGAGAGTATCCTGCAGATATGCAATGCAAGGATAAGTTTCTCCTACAAAGTACAATAGTCCCACCAAATACTGATGTAGATGAGCTTCCTTCTGATGCTTTCAATAAGGACAGTGGAAGGACAATTGAGGAATGCAAGCTTAAGGTTATCTATATTTCTCCTGCATCAGCCTTGGGGAACTCAGAAGATGAAAAGAACTCATCTCAAAGCTCCTCGGCTGTACAACGTCTGAAGGAAGAAAGAGACATGGCTATAAGGCAAACCCATCAATTACAACAGGAACTGGACATGATGAAGAGACGAAAATATCGGAAAGGCGATCCTGGCTTCTCGTTTACTTTCGCGATCTTCGTGGGGATCGTTGGGATCATGGCAGGTTTCCTCTTGAATCTCACATTATCTTCACCATCTACGGAATGA

Protein sequence

MSAGGGNRLISVQPDELKFQIEFEKQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTGVVQPWDSCIIRVTLQAQREYPADMQCKDKFLLQSTIVPPNTDVDELPSDAFNKDSGRTIEECKLKVIYISPASALGNSEDEKNSSQSSSAVQRLKEERDMAIRQTHQLQQELDMMKRRKYRKGDPGFSFTFAIFVGIVGIMAGFLLNLTLSSPSTE
Homology
BLAST of Bhi11G000062 vs. TAIR 10
Match: AT5G47180.1 (Plant VAMP (vesicle-associated membrane protein) family protein )

HSP 1 Score: 290.0 bits (741), Expect = 1.5e-78
Identity = 145/220 (65.91%), Postives = 176/220 (80.00%), Query Frame = 0

Query: 1   MSAGGGNRLISVQPDELKFQIEFEKQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTG 60
           M+  G N+LIS+QPDELKF  E EKQS+CDLKVAN TE++VAFKVKTTSPKKYFVRPNTG
Sbjct: 1   MTGVGENQLISIQPDELKFLFELEKQSYCDLKVANKTENYVAFKVKTTSPKKYFVRPNTG 60

Query: 61  VVQPWDSCIIRVTLQAQREYPADMQCKDKFLLQSTIVPPNTDVDELPSDAFNKDSGRTIE 120
           V+QPWDSCIIRVTLQAQREYP DMQCKDKFLLQSTIVPP+TDVDELP D F KDSG+T+ 
Sbjct: 61  VIQPWDSCIIRVTLQAQREYPPDMQCKDKFLLQSTIVPPHTDVDELPQDTFTKDSGKTLT 120

Query: 121 ECKLKVIYISPASALGNSED-----EKNSSQSSSAVQRLKEERDMAIRQTHQLQQELDMM 180
           ECKLKV YI+P++   +SE      +  SS++ S +QRLKEERD A++QT QLQ EL+ +
Sbjct: 121 ECKLKVSYITPSTTQRSSESGATNGDGQSSETISTIQRLKEERDAAVKQTQQLQHELETV 180

Query: 181 KRRK-YRKGDPGFSFTFAIFVGIVGIMAGFLLNLTLSSPS 215
           +RR+  R    G S   A  VG++G++ GF+L LTL+SP+
Sbjct: 181 RRRRNQRNSGNGLSLKLAAMVGLIGLIIGFILKLTLASPT 220

BLAST of Bhi11G000062 vs. TAIR 10
Match: AT5G47180.2 (Plant VAMP (vesicle-associated membrane protein) family protein )

HSP 1 Score: 290.0 bits (741), Expect = 1.5e-78
Identity = 145/220 (65.91%), Postives = 176/220 (80.00%), Query Frame = 0

Query: 1   MSAGGGNRLISVQPDELKFQIEFEKQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTG 60
           M+  G N+LIS+QPDELKF  E EKQS+CDLKVAN TE++VAFKVKTTSPKKYFVRPNTG
Sbjct: 1   MTGVGENQLISIQPDELKFLFELEKQSYCDLKVANKTENYVAFKVKTTSPKKYFVRPNTG 60

Query: 61  VVQPWDSCIIRVTLQAQREYPADMQCKDKFLLQSTIVPPNTDVDELPSDAFNKDSGRTIE 120
           V+QPWDSCIIRVTLQAQREYP DMQCKDKFLLQSTIVPP+TDVDELP D F KDSG+T+ 
Sbjct: 61  VIQPWDSCIIRVTLQAQREYPPDMQCKDKFLLQSTIVPPHTDVDELPQDTFTKDSGKTLT 120

Query: 121 ECKLKVIYISPASALGNSED-----EKNSSQSSSAVQRLKEERDMAIRQTHQLQQELDMM 180
           ECKLKV YI+P++   +SE      +  SS++ S +QRLKEERD A++QT QLQ EL+ +
Sbjct: 121 ECKLKVSYITPSTTQRSSESGATNGDGQSSETISTIQRLKEERDAAVKQTQQLQHELETV 180

Query: 181 KRRK-YRKGDPGFSFTFAIFVGIVGIMAGFLLNLTLSSPS 215
           +RR+  R    G S   A  VG++G++ GF+L LTL+SP+
Sbjct: 181 RRRRNQRNSGNGLSLKLAAMVGLIGLIIGFILKLTLASPT 220

BLAST of Bhi11G000062 vs. TAIR 10
Match: AT2G45140.1 (plant VAP homolog 12 )

HSP 1 Score: 172.9 bits (437), Expect = 2.7e-43
Identity = 90/237 (37.97%), Postives = 140/237 (59.07%), Query Frame = 0

Query: 7   NRLISVQPDELKFQIEFEKQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTGVVQPWD 66
           N L+++ P +L+F  E +KQ  C L + N T+++VAFKVKTT+PKKY VRPNTGVV P  
Sbjct: 3   NELLTIDPVDLQFPFELKKQISCSLYLGNKTDNYVAFKVKTTNPKKYCVRPNTGVVHPRS 62

Query: 67  SCIIRVTLQAQREYPADMQCKDKFLLQSTIVPPNTDVDELPSDAFNKDSGRTIEECKLKV 126
           S  + VT+QAQ+E PAD+QCKDKFLLQ  +  P     ++  + F+K++G  +EE KL+V
Sbjct: 63  SSEVLVTMQAQKEAPADLQCKDKFLLQCVVASPGATPKDVTHEMFSKEAGHRVEETKLRV 122

Query: 127 IYISP--------------------ASALGNSED--------------EKNSSQSSSAVQ 186
           +Y++P                     S  GN+ D              + NSS++ + V 
Sbjct: 123 VYVAPPRPPSPVREGSEEGSSPRASVSDNGNASDFTAAPRFSADRVDAQDNSSEARALVT 182

Query: 187 RLKEERDMAIRQTHQLQQELDMMKRRKYRKGDPGFSFTFAIFVGIVGIMAGFLLNLT 210
           +L EE++ A++  ++LQQELD ++R   R    G  F + + VG++G++ G+++  T
Sbjct: 183 KLTEEKNSAVQLNNRLQQELDQLRRESKRSKSGGIPFMYVLLVGLIGLILGYIMKRT 239

BLAST of Bhi11G000062 vs. TAIR 10
Match: AT4G00170.1 (Plant VAMP (vesicle-associated membrane protein) family protein )

HSP 1 Score: 169.5 bits (428), Expect = 3.0e-42
Identity = 94/232 (40.52%), Postives = 140/232 (60.34%), Query Frame = 0

Query: 9   LISVQPDELKFQIEFEKQSFCDLKVANNTEHH-VAFKVKTTSPKKYFVRPNTGVVQPWDS 68
           L+++ P ELKF  E +KQS C +++ N T    VAFKVKTT+P+KY VRPNTGVV P DS
Sbjct: 6   LVNIHPTELKFPFELKKQSSCSMQLTNKTTTQCVAFKVKTTNPRKYCVRPNTGVVLPGDS 65

Query: 69  CIIRVTLQAQREYPADMQCKDKFLLQSTIVPPNTDVDELPSDAFNKDSGRTIEECKLKVI 128
           C + VT+QAQ+E P DMQCKDKFL+Q+ +V   T   E+ ++ FNK++GR IE+ KL+V+
Sbjct: 66  CNVTVTMQAQKEAPLDMQCKDKFLVQTVVVSDGTTSKEVLAEMFNKEAGRVIEDFKLRVV 125

Query: 129 YI----------------SPASALGN----------------SEDEKNSSQSSSAVQRLK 188
           YI                SP ++L +                 E  + SS++ S + +L 
Sbjct: 126 YIPANPPSPVPEGSEEGNSPMASLNDIASQSASLFDDVSRTFEETSEKSSEAWSMISKLT 185

Query: 189 EERDMAIRQTHQLQQELDMMKRRKYRKGDPGFSFTFAIFVGIVGIMAGFLLN 208
           EE+  A +Q+ +L+ EL+M+++   +K   G S    + VG++G + G+LLN
Sbjct: 186 EEKTSATQQSQKLRLELEMLRKETSKKQSGGHSLLLMLLVGLLGCVIGYLLN 237

BLAST of Bhi11G000062 vs. TAIR 10
Match: AT3G60600.1 (vesicle associated protein )

HSP 1 Score: 167.9 bits (424), Expect = 8.8e-42
Identity = 88/237 (37.13%), Postives = 139/237 (58.65%), Query Frame = 0

Query: 7   NRLISVQPDELKFQIEFEKQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTGVVQPWD 66
           + L++V+P +L+F  E +KQ  C L + N T+++VAFKVKTT+PKKY VRPNTGVV P  
Sbjct: 20  SELLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVLPRS 79

Query: 67  SCIIRVTLQAQREYPADMQCKDKFLLQSTIVPPNTDVDELPSDAFNKDSGRTIEECKLKV 126
           +C + VT+QAQ+E P+DMQCKDKFLLQ  I  P     E+  + F+K++G  +EE KL+V
Sbjct: 80  TCEVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETKLRV 139

Query: 127 IYISP------------------ASALGNSE----------------DEKNSSQSSSAVQ 186
            Y++P                  AS   N                   ++N+S++ + + 
Sbjct: 140 TYVAPPRPPSPVHEGSEEGSSPRASVSDNGHGSEFSFERFIVDNKAGHQENTSEARALIT 199

Query: 187 RLKEERDMAIRQTHQLQQELDMMKRRKYRKGDPGFSFTFAIFVGIVGIMAGFLLNLT 210
           +L EE+  AI+  ++LQ+ELD ++R   +    G  F + + VG++G++ G+++  T
Sbjct: 200 KLTEEKQSAIQLNNRLQRELDQLRRESKKSQSGGIPFMYVLLVGLIGLILGYIMKRT 256

BLAST of Bhi11G000062 vs. ExPASy Swiss-Prot
Match: Q9LVU1 (Vesicle-associated protein 2-1 OS=Arabidopsis thaliana OX=3702 GN=PVA21 PE=1 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 2.2e-77
Identity = 145/220 (65.91%), Postives = 176/220 (80.00%), Query Frame = 0

Query: 1   MSAGGGNRLISVQPDELKFQIEFEKQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTG 60
           M+  G N+LIS+QPDELKF  E EKQS+CDLKVAN TE++VAFKVKTTSPKKYFVRPNTG
Sbjct: 1   MTGVGENQLISIQPDELKFLFELEKQSYCDLKVANKTENYVAFKVKTTSPKKYFVRPNTG 60

Query: 61  VVQPWDSCIIRVTLQAQREYPADMQCKDKFLLQSTIVPPNTDVDELPSDAFNKDSGRTIE 120
           V+QPWDSCIIRVTLQAQREYP DMQCKDKFLLQSTIVPP+TDVDELP D F KDSG+T+ 
Sbjct: 61  VIQPWDSCIIRVTLQAQREYPPDMQCKDKFLLQSTIVPPHTDVDELPQDTFTKDSGKTLT 120

Query: 121 ECKLKVIYISPASALGNSED-----EKNSSQSSSAVQRLKEERDMAIRQTHQLQQELDMM 180
           ECKLKV YI+P++   +SE      +  SS++ S +QRLKEERD A++QT QLQ EL+ +
Sbjct: 121 ECKLKVSYITPSTTQRSSESGATNGDGQSSETISTIQRLKEERDAAVKQTQQLQHELETV 180

Query: 181 KRRK-YRKGDPGFSFTFAIFVGIVGIMAGFLLNLTLSSPS 215
           +RR+  R    G S   A  VG++G++ GF+L LTL+SP+
Sbjct: 181 RRRRNQRNSGNGLSLKLAAMVGLIGLIIGFILKLTLASPT 220

BLAST of Bhi11G000062 vs. ExPASy Swiss-Prot
Match: Q9SHC8 (Vesicle-associated protein 1-2 OS=Arabidopsis thaliana OX=3702 GN=PVA12 PE=1 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 3.8e-42
Identity = 90/237 (37.97%), Postives = 140/237 (59.07%), Query Frame = 0

Query: 7   NRLISVQPDELKFQIEFEKQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTGVVQPWD 66
           N L+++ P +L+F  E +KQ  C L + N T+++VAFKVKTT+PKKY VRPNTGVV P  
Sbjct: 3   NELLTIDPVDLQFPFELKKQISCSLYLGNKTDNYVAFKVKTTNPKKYCVRPNTGVVHPRS 62

Query: 67  SCIIRVTLQAQREYPADMQCKDKFLLQSTIVPPNTDVDELPSDAFNKDSGRTIEECKLKV 126
           S  + VT+QAQ+E PAD+QCKDKFLLQ  +  P     ++  + F+K++G  +EE KL+V
Sbjct: 63  SSEVLVTMQAQKEAPADLQCKDKFLLQCVVASPGATPKDVTHEMFSKEAGHRVEETKLRV 122

Query: 127 IYISP--------------------ASALGNSED--------------EKNSSQSSSAVQ 186
           +Y++P                     S  GN+ D              + NSS++ + V 
Sbjct: 123 VYVAPPRPPSPVREGSEEGSSPRASVSDNGNASDFTAAPRFSADRVDAQDNSSEARALVT 182

Query: 187 RLKEERDMAIRQTHQLQQELDMMKRRKYRKGDPGFSFTFAIFVGIVGIMAGFLLNLT 210
           +L EE++ A++  ++LQQELD ++R   R    G  F + + VG++G++ G+++  T
Sbjct: 183 KLTEEKNSAVQLNNRLQQELDQLRRESKRSKSGGIPFMYVLLVGLIGLILGYIMKRT 239

BLAST of Bhi11G000062 vs. ExPASy Swiss-Prot
Match: Q84WW5 (Vesicle-associated protein 1-3 OS=Arabidopsis thaliana OX=3702 GN=PVA13 PE=2 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 4.2e-41
Identity = 94/232 (40.52%), Postives = 140/232 (60.34%), Query Frame = 0

Query: 9   LISVQPDELKFQIEFEKQSFCDLKVANNTEHH-VAFKVKTTSPKKYFVRPNTGVVQPWDS 68
           L+++ P ELKF  E +KQS C +++ N T    VAFKVKTT+P+KY VRPNTGVV P DS
Sbjct: 6   LVNIHPTELKFPFELKKQSSCSMQLTNKTTTQCVAFKVKTTNPRKYCVRPNTGVVLPGDS 65

Query: 69  CIIRVTLQAQREYPADMQCKDKFLLQSTIVPPNTDVDELPSDAFNKDSGRTIEECKLKVI 128
           C + VT+QAQ+E P DMQCKDKFL+Q+ +V   T   E+ ++ FNK++GR IE+ KL+V+
Sbjct: 66  CNVTVTMQAQKEAPLDMQCKDKFLVQTVVVSDGTTSKEVLAEMFNKEAGRVIEDFKLRVV 125

Query: 129 YI----------------SPASALGN----------------SEDEKNSSQSSSAVQRLK 188
           YI                SP ++L +                 E  + SS++ S + +L 
Sbjct: 126 YIPANPPSPVPEGSEEGNSPMASLNDIASQSASLFDDVSRTFEETSEKSSEAWSMISKLT 185

Query: 189 EERDMAIRQTHQLQQELDMMKRRKYRKGDPGFSFTFAIFVGIVGIMAGFLLN 208
           EE+  A +Q+ +L+ EL+M+++   +K   G S    + VG++G + G+LLN
Sbjct: 186 EEKTSATQQSQKLRLELEMLRKETSKKQSGGHSLLLMLLVGLLGCVIGYLLN 237

BLAST of Bhi11G000062 vs. ExPASy Swiss-Prot
Match: Q8VZ95 (Vesicle-associated protein 1-1 OS=Arabidopsis thaliana OX=3702 GN=PVA11 PE=1 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 1.2e-40
Identity = 88/237 (37.13%), Postives = 139/237 (58.65%), Query Frame = 0

Query: 7   NRLISVQPDELKFQIEFEKQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTGVVQPWD 66
           + L++V+P +L+F  E +KQ  C L + N T+++VAFKVKTT+PKKY VRPNTGVV P  
Sbjct: 20  SELLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVLPRS 79

Query: 67  SCIIRVTLQAQREYPADMQCKDKFLLQSTIVPPNTDVDELPSDAFNKDSGRTIEECKLKV 126
           +C + VT+QAQ+E P+DMQCKDKFLLQ  I  P     E+  + F+K++G  +EE KL+V
Sbjct: 80  TCEVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETKLRV 139

Query: 127 IYISP------------------ASALGNSE----------------DEKNSSQSSSAVQ 186
            Y++P                  AS   N                   ++N+S++ + + 
Sbjct: 140 TYVAPPRPPSPVHEGSEEGSSPRASVSDNGHGSEFSFERFIVDNKAGHQENTSEARALIT 199

Query: 187 RLKEERDMAIRQTHQLQQELDMMKRRKYRKGDPGFSFTFAIFVGIVGIMAGFLLNLT 210
           +L EE+  AI+  ++LQ+ELD ++R   +    G  F + + VG++G++ G+++  T
Sbjct: 200 KLTEEKQSAIQLNNRLQRELDQLRRESKKSQSGGIPFMYVLLVGLIGLILGYIMKRT 256

BLAST of Bhi11G000062 vs. ExPASy Swiss-Prot
Match: Q9SYC9 (Vesicle-associated protein 1-4 OS=Arabidopsis thaliana OX=3702 GN=PVA14 PE=2 SV=1)

HSP 1 Score: 130.6 bits (327), Expect = 2.2e-29
Identity = 63/145 (43.45%), Postives = 95/145 (65.52%), Query Frame = 0

Query: 7   NRLISVQPDELKFQIEFEKQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTGVVQPWD 66
           + L+ + P +++F IE  K+  C L + N TE++VAFK KTT+ KKY+VRPN GVV P  
Sbjct: 174 DELLIIDPVDVQFPIELNKKVSCSLNLTNKTENYVAFKAKTTNAKKYYVRPNVGVVLPRS 233

Query: 67  SCIIRVTLQAQREYPADMQCKDKFLLQSTIVPPNTDVDELPSDAFNKDSGRTIEECKLKV 126
           SC + V +QA +E PADMQC+DK L Q  +V P T   ++ S+ F+K++G   EE +LKV
Sbjct: 234 SCEVLVIMQALKEAPADMQCRDKLLFQCKVVEPETTAKDVTSEMFSKEAGHPAEETRLKV 293

Query: 127 IYIS---PASALGNSEDEKNSSQSS 149
           +Y++   P S +    +E +S ++S
Sbjct: 294 MYVTPPQPPSPVQEGTEEGSSPRAS 318

BLAST of Bhi11G000062 vs. NCBI nr
Match: XP_038905973.1 (vesicle-associated protein 2-1 [Benincasa hispida])

HSP 1 Score: 429.1 bits (1102), Expect = 2.3e-116
Identity = 216/216 (100.00%), Postives = 216/216 (100.00%), Query Frame = 0

Query: 1   MSAGGGNRLISVQPDELKFQIEFEKQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTG 60
           MSAGGGNRLISVQPDELKFQIEFEKQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTG
Sbjct: 1   MSAGGGNRLISVQPDELKFQIEFEKQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTG 60

Query: 61  VVQPWDSCIIRVTLQAQREYPADMQCKDKFLLQSTIVPPNTDVDELPSDAFNKDSGRTIE 120
           VVQPWDSCIIRVTLQAQREYPADMQCKDKFLLQSTIVPPNTDVDELPSDAFNKDSGRTIE
Sbjct: 61  VVQPWDSCIIRVTLQAQREYPADMQCKDKFLLQSTIVPPNTDVDELPSDAFNKDSGRTIE 120

Query: 121 ECKLKVIYISPASALGNSEDEKNSSQSSSAVQRLKEERDMAIRQTHQLQQELDMMKRRKY 180
           ECKLKVIYISPASALGNSEDEKNSSQSSSAVQRLKEERDMAIRQTHQLQQELDMMKRRKY
Sbjct: 121 ECKLKVIYISPASALGNSEDEKNSSQSSSAVQRLKEERDMAIRQTHQLQQELDMMKRRKY 180

Query: 181 RKGDPGFSFTFAIFVGIVGIMAGFLLNLTLSSPSTE 217
           RKGDPGFSFTFAIFVGIVGIMAGFLLNLTLSSPSTE
Sbjct: 181 RKGDPGFSFTFAIFVGIVGIMAGFLLNLTLSSPSTE 216

BLAST of Bhi11G000062 vs. NCBI nr
Match: XP_008448784.1 (PREDICTED: vesicle-associated protein 2-1 [Cucumis melo] >KAA0041052.1 vesicle-associated protein 2-1 [Cucumis melo var. makuwa] >TYK12062.1 vesicle-associated protein 2-1 [Cucumis melo var. makuwa])

HSP 1 Score: 417.9 bits (1073), Expect = 5.2e-113
Identity = 210/216 (97.22%), Postives = 211/216 (97.69%), Query Frame = 0

Query: 1   MSAGGGNRLISVQPDELKFQIEFEKQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTG 60
           MSAGGGNRLISVQPDELKFQIE +KQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTG
Sbjct: 1   MSAGGGNRLISVQPDELKFQIELDKQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTG 60

Query: 61  VVQPWDSCIIRVTLQAQREYPADMQCKDKFLLQSTIVPPNTDVDELPSDAFNKDSGRTIE 120
           VVQPWDSCIIRVTLQA REYPADMQCKDKFLLQSTIVPPNTDVDELPSDAFNKDSGRTIE
Sbjct: 61  VVQPWDSCIIRVTLQAMREYPADMQCKDKFLLQSTIVPPNTDVDELPSDAFNKDSGRTIE 120

Query: 121 ECKLKVIYISPASALGNSEDEKNSSQSSSAVQRLKEERDMAIRQTHQLQQELDMMKRRKY 180
           ECKLKVIYISP SALGNSEDEKN SQSSSAV RLKEERDMAIRQTHQLQQELDMMKRRKY
Sbjct: 121 ECKLKVIYISPTSALGNSEDEKNLSQSSSAVHRLKEERDMAIRQTHQLQQELDMMKRRKY 180

Query: 181 RKGDPGFSFTFAIFVGIVGIMAGFLLNLTLSSPSTE 217
           RKGDPGFSFTFAIFVGIVGIMAGFLLNLTLSSPSTE
Sbjct: 181 RKGDPGFSFTFAIFVGIVGIMAGFLLNLTLSSPSTE 216

BLAST of Bhi11G000062 vs. NCBI nr
Match: XP_004147234.1 (vesicle-associated protein 2-1 [Cucumis sativus] >KGN55827.1 hypothetical protein Csa_011407 [Cucumis sativus])

HSP 1 Score: 414.8 bits (1065), Expect = 4.4e-112
Identity = 207/216 (95.83%), Postives = 211/216 (97.69%), Query Frame = 0

Query: 1   MSAGGGNRLISVQPDELKFQIEFEKQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTG 60
           MSAGGGNRLISVQPDELKFQIE +KQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTG
Sbjct: 1   MSAGGGNRLISVQPDELKFQIELDKQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTG 60

Query: 61  VVQPWDSCIIRVTLQAQREYPADMQCKDKFLLQSTIVPPNTDVDELPSDAFNKDSGRTIE 120
           VVQPWDSCIIRVTLQA REYPADMQCKDKFLLQSTIVPPNTDVDELPSDAFNKDSG+TIE
Sbjct: 61  VVQPWDSCIIRVTLQAMREYPADMQCKDKFLLQSTIVPPNTDVDELPSDAFNKDSGKTIE 120

Query: 121 ECKLKVIYISPASALGNSEDEKNSSQSSSAVQRLKEERDMAIRQTHQLQQELDMMKRRKY 180
           ECKLKVIYISP SALGNSEDEKNSSQSSSAV RL+EERDMAIRQTHQLQQELDMMKRRK+
Sbjct: 121 ECKLKVIYISPTSALGNSEDEKNSSQSSSAVHRLREERDMAIRQTHQLQQELDMMKRRKF 180

Query: 181 RKGDPGFSFTFAIFVGIVGIMAGFLLNLTLSSPSTE 217
           RKGDPGFSFTFAIFVG VGIMAGFLLNLTLSSPSTE
Sbjct: 181 RKGDPGFSFTFAIFVGFVGIMAGFLLNLTLSSPSTE 216

BLAST of Bhi11G000062 vs. NCBI nr
Match: XP_023551809.1 (vesicle-associated protein 2-1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 407.5 bits (1046), Expect = 7.1e-110
Identity = 202/216 (93.52%), Postives = 211/216 (97.69%), Query Frame = 0

Query: 1   MSAGGGNRLISVQPDELKFQIEFEKQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTG 60
           MSAG GNRLIS+QPDELKFQIE EKQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTG
Sbjct: 1   MSAGSGNRLISIQPDELKFQIELEKQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTG 60

Query: 61  VVQPWDSCIIRVTLQAQREYPADMQCKDKFLLQSTIVPPNTDVDELPSDAFNKDSGRTIE 120
           VVQPWDSCIIRVTLQAQREYPADMQC+DKFLLQSTIVPPNTDVDELP+DAFNKDSGR IE
Sbjct: 61  VVQPWDSCIIRVTLQAQREYPADMQCRDKFLLQSTIVPPNTDVDELPADAFNKDSGRAIE 120

Query: 121 ECKLKVIYISPASALGNSEDEKNSSQSSSAVQRLKEERDMAIRQTHQLQQELDMMKRRKY 180
           ECKLKVIYIS  S+LGNSEDEKNSSQSS+A+QRLKE+RD+AIRQTHQLQQELDMMKRRKY
Sbjct: 121 ECKLKVIYISSTSSLGNSEDEKNSSQSSTALQRLKEDRDIAIRQTHQLQQELDMMKRRKY 180

Query: 181 RKGDPGFSFTFAIFVGIVGIMAGFLLNLTLSSPSTE 217
           RKGDPGFSFTFAIFVGIVGI+AGFLLNLTLSSPSTE
Sbjct: 181 RKGDPGFSFTFAIFVGIVGIIAGFLLNLTLSSPSTE 216

BLAST of Bhi11G000062 vs. NCBI nr
Match: XP_022923370.1 (vesicle-associated protein 2-1 [Cucurbita moschata] >XP_022965179.1 vesicle-associated protein 2-1-like [Cucurbita maxima] >KAG6577660.1 Vesicle-associated protein 2-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 407.5 bits (1046), Expect = 7.1e-110
Identity = 202/216 (93.52%), Postives = 210/216 (97.22%), Query Frame = 0

Query: 1   MSAGGGNRLISVQPDELKFQIEFEKQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTG 60
           MSAG GNRLIS+QPDELKFQIE EKQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTG
Sbjct: 1   MSAGSGNRLISIQPDELKFQIELEKQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTG 60

Query: 61  VVQPWDSCIIRVTLQAQREYPADMQCKDKFLLQSTIVPPNTDVDELPSDAFNKDSGRTIE 120
           VVQPWDSCIIRVTLQAQREYPADMQC+DKFLLQSTIVPPNTDVDELP+DAFNKDSGR IE
Sbjct: 61  VVQPWDSCIIRVTLQAQREYPADMQCRDKFLLQSTIVPPNTDVDELPADAFNKDSGRAIE 120

Query: 121 ECKLKVIYISPASALGNSEDEKNSSQSSSAVQRLKEERDMAIRQTHQLQQELDMMKRRKY 180
           ECKLKVIYIS  S+LGNSEDEKNSSQSS+A+QRLKE+RDMAIRQTHQLQQELDMMKRRKY
Sbjct: 121 ECKLKVIYISSTSSLGNSEDEKNSSQSSTALQRLKEDRDMAIRQTHQLQQELDMMKRRKY 180

Query: 181 RKGDPGFSFTFAIFVGIVGIMAGFLLNLTLSSPSTE 217
           RKGDPGFSFTFAIFVGIVGI+AGFLLN TLSSPSTE
Sbjct: 181 RKGDPGFSFTFAIFVGIVGIIAGFLLNFTLSSPSTE 216

BLAST of Bhi11G000062 vs. ExPASy TrEMBL
Match: A0A5A7TC60 (Vesicle-associated protein 2-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1017G00770 PE=4 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 2.5e-113
Identity = 210/216 (97.22%), Postives = 211/216 (97.69%), Query Frame = 0

Query: 1   MSAGGGNRLISVQPDELKFQIEFEKQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTG 60
           MSAGGGNRLISVQPDELKFQIE +KQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTG
Sbjct: 1   MSAGGGNRLISVQPDELKFQIELDKQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTG 60

Query: 61  VVQPWDSCIIRVTLQAQREYPADMQCKDKFLLQSTIVPPNTDVDELPSDAFNKDSGRTIE 120
           VVQPWDSCIIRVTLQA REYPADMQCKDKFLLQSTIVPPNTDVDELPSDAFNKDSGRTIE
Sbjct: 61  VVQPWDSCIIRVTLQAMREYPADMQCKDKFLLQSTIVPPNTDVDELPSDAFNKDSGRTIE 120

Query: 121 ECKLKVIYISPASALGNSEDEKNSSQSSSAVQRLKEERDMAIRQTHQLQQELDMMKRRKY 180
           ECKLKVIYISP SALGNSEDEKN SQSSSAV RLKEERDMAIRQTHQLQQELDMMKRRKY
Sbjct: 121 ECKLKVIYISPTSALGNSEDEKNLSQSSSAVHRLKEERDMAIRQTHQLQQELDMMKRRKY 180

Query: 181 RKGDPGFSFTFAIFVGIVGIMAGFLLNLTLSSPSTE 217
           RKGDPGFSFTFAIFVGIVGIMAGFLLNLTLSSPSTE
Sbjct: 181 RKGDPGFSFTFAIFVGIVGIMAGFLLNLTLSSPSTE 216

BLAST of Bhi11G000062 vs. ExPASy TrEMBL
Match: A0A1S3BLE3 (vesicle-associated protein 2-1 OS=Cucumis melo OX=3656 GN=LOC103490849 PE=4 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 2.5e-113
Identity = 210/216 (97.22%), Postives = 211/216 (97.69%), Query Frame = 0

Query: 1   MSAGGGNRLISVQPDELKFQIEFEKQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTG 60
           MSAGGGNRLISVQPDELKFQIE +KQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTG
Sbjct: 1   MSAGGGNRLISVQPDELKFQIELDKQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTG 60

Query: 61  VVQPWDSCIIRVTLQAQREYPADMQCKDKFLLQSTIVPPNTDVDELPSDAFNKDSGRTIE 120
           VVQPWDSCIIRVTLQA REYPADMQCKDKFLLQSTIVPPNTDVDELPSDAFNKDSGRTIE
Sbjct: 61  VVQPWDSCIIRVTLQAMREYPADMQCKDKFLLQSTIVPPNTDVDELPSDAFNKDSGRTIE 120

Query: 121 ECKLKVIYISPASALGNSEDEKNSSQSSSAVQRLKEERDMAIRQTHQLQQELDMMKRRKY 180
           ECKLKVIYISP SALGNSEDEKN SQSSSAV RLKEERDMAIRQTHQLQQELDMMKRRKY
Sbjct: 121 ECKLKVIYISPTSALGNSEDEKNLSQSSSAVHRLKEERDMAIRQTHQLQQELDMMKRRKY 180

Query: 181 RKGDPGFSFTFAIFVGIVGIMAGFLLNLTLSSPSTE 217
           RKGDPGFSFTFAIFVGIVGIMAGFLLNLTLSSPSTE
Sbjct: 181 RKGDPGFSFTFAIFVGIVGIMAGFLLNLTLSSPSTE 216

BLAST of Bhi11G000062 vs. ExPASy TrEMBL
Match: A0A0A0L5E3 (MSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G017210 PE=4 SV=1)

HSP 1 Score: 414.8 bits (1065), Expect = 2.1e-112
Identity = 207/216 (95.83%), Postives = 211/216 (97.69%), Query Frame = 0

Query: 1   MSAGGGNRLISVQPDELKFQIEFEKQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTG 60
           MSAGGGNRLISVQPDELKFQIE +KQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTG
Sbjct: 1   MSAGGGNRLISVQPDELKFQIELDKQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTG 60

Query: 61  VVQPWDSCIIRVTLQAQREYPADMQCKDKFLLQSTIVPPNTDVDELPSDAFNKDSGRTIE 120
           VVQPWDSCIIRVTLQA REYPADMQCKDKFLLQSTIVPPNTDVDELPSDAFNKDSG+TIE
Sbjct: 61  VVQPWDSCIIRVTLQAMREYPADMQCKDKFLLQSTIVPPNTDVDELPSDAFNKDSGKTIE 120

Query: 121 ECKLKVIYISPASALGNSEDEKNSSQSSSAVQRLKEERDMAIRQTHQLQQELDMMKRRKY 180
           ECKLKVIYISP SALGNSEDEKNSSQSSSAV RL+EERDMAIRQTHQLQQELDMMKRRK+
Sbjct: 121 ECKLKVIYISPTSALGNSEDEKNSSQSSSAVHRLREERDMAIRQTHQLQQELDMMKRRKF 180

Query: 181 RKGDPGFSFTFAIFVGIVGIMAGFLLNLTLSSPSTE 217
           RKGDPGFSFTFAIFVG VGIMAGFLLNLTLSSPSTE
Sbjct: 181 RKGDPGFSFTFAIFVGFVGIMAGFLLNLTLSSPSTE 216

BLAST of Bhi11G000062 vs. ExPASy TrEMBL
Match: A0A6J1HQA2 (vesicle-associated protein 2-1-like OS=Cucurbita maxima OX=3661 GN=LOC111465113 PE=4 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 3.4e-110
Identity = 202/216 (93.52%), Postives = 210/216 (97.22%), Query Frame = 0

Query: 1   MSAGGGNRLISVQPDELKFQIEFEKQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTG 60
           MSAG GNRLIS+QPDELKFQIE EKQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTG
Sbjct: 1   MSAGSGNRLISIQPDELKFQIELEKQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTG 60

Query: 61  VVQPWDSCIIRVTLQAQREYPADMQCKDKFLLQSTIVPPNTDVDELPSDAFNKDSGRTIE 120
           VVQPWDSCIIRVTLQAQREYPADMQC+DKFLLQSTIVPPNTDVDELP+DAFNKDSGR IE
Sbjct: 61  VVQPWDSCIIRVTLQAQREYPADMQCRDKFLLQSTIVPPNTDVDELPADAFNKDSGRAIE 120

Query: 121 ECKLKVIYISPASALGNSEDEKNSSQSSSAVQRLKEERDMAIRQTHQLQQELDMMKRRKY 180
           ECKLKVIYIS  S+LGNSEDEKNSSQSS+A+QRLKE+RDMAIRQTHQLQQELDMMKRRKY
Sbjct: 121 ECKLKVIYISSTSSLGNSEDEKNSSQSSTALQRLKEDRDMAIRQTHQLQQELDMMKRRKY 180

Query: 181 RKGDPGFSFTFAIFVGIVGIMAGFLLNLTLSSPSTE 217
           RKGDPGFSFTFAIFVGIVGI+AGFLLN TLSSPSTE
Sbjct: 181 RKGDPGFSFTFAIFVGIVGIIAGFLLNFTLSSPSTE 216

BLAST of Bhi11G000062 vs. ExPASy TrEMBL
Match: A0A6J1EBL8 (vesicle-associated protein 2-1 OS=Cucurbita moschata OX=3662 GN=LOC111431088 PE=4 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 3.4e-110
Identity = 202/216 (93.52%), Postives = 210/216 (97.22%), Query Frame = 0

Query: 1   MSAGGGNRLISVQPDELKFQIEFEKQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTG 60
           MSAG GNRLIS+QPDELKFQIE EKQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTG
Sbjct: 1   MSAGSGNRLISIQPDELKFQIELEKQSFCDLKVANNTEHHVAFKVKTTSPKKYFVRPNTG 60

Query: 61  VVQPWDSCIIRVTLQAQREYPADMQCKDKFLLQSTIVPPNTDVDELPSDAFNKDSGRTIE 120
           VVQPWDSCIIRVTLQAQREYPADMQC+DKFLLQSTIVPPNTDVDELP+DAFNKDSGR IE
Sbjct: 61  VVQPWDSCIIRVTLQAQREYPADMQCRDKFLLQSTIVPPNTDVDELPADAFNKDSGRAIE 120

Query: 121 ECKLKVIYISPASALGNSEDEKNSSQSSSAVQRLKEERDMAIRQTHQLQQELDMMKRRKY 180
           ECKLKVIYIS  S+LGNSEDEKNSSQSS+A+QRLKE+RDMAIRQTHQLQQELDMMKRRKY
Sbjct: 121 ECKLKVIYISSTSSLGNSEDEKNSSQSSTALQRLKEDRDMAIRQTHQLQQELDMMKRRKY 180

Query: 181 RKGDPGFSFTFAIFVGIVGIMAGFLLNLTLSSPSTE 217
           RKGDPGFSFTFAIFVGIVGI+AGFLLN TLSSPSTE
Sbjct: 181 RKGDPGFSFTFAIFVGIVGIIAGFLLNFTLSSPSTE 216

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G47180.11.5e-7865.91Plant VAMP (vesicle-associated membrane protein) family protein [more]
AT5G47180.21.5e-7865.91Plant VAMP (vesicle-associated membrane protein) family protein [more]
AT2G45140.12.7e-4337.97plant VAP homolog 12 [more]
AT4G00170.13.0e-4240.52Plant VAMP (vesicle-associated membrane protein) family protein [more]
AT3G60600.18.8e-4237.13vesicle associated protein [more]
Match NameE-valueIdentityDescription
Q9LVU12.2e-7765.91Vesicle-associated protein 2-1 OS=Arabidopsis thaliana OX=3702 GN=PVA21 PE=1 SV=... [more]
Q9SHC83.8e-4237.97Vesicle-associated protein 1-2 OS=Arabidopsis thaliana OX=3702 GN=PVA12 PE=1 SV=... [more]
Q84WW54.2e-4140.52Vesicle-associated protein 1-3 OS=Arabidopsis thaliana OX=3702 GN=PVA13 PE=2 SV=... [more]
Q8VZ951.2e-4037.13Vesicle-associated protein 1-1 OS=Arabidopsis thaliana OX=3702 GN=PVA11 PE=1 SV=... [more]
Q9SYC92.2e-2943.45Vesicle-associated protein 1-4 OS=Arabidopsis thaliana OX=3702 GN=PVA14 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
XP_038905973.12.3e-116100.00vesicle-associated protein 2-1 [Benincasa hispida][more]
XP_008448784.15.2e-11397.22PREDICTED: vesicle-associated protein 2-1 [Cucumis melo] >KAA0041052.1 vesicle-a... [more]
XP_004147234.14.4e-11295.83vesicle-associated protein 2-1 [Cucumis sativus] >KGN55827.1 hypothetical protei... [more]
XP_023551809.17.1e-11093.52vesicle-associated protein 2-1-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022923370.17.1e-11093.52vesicle-associated protein 2-1 [Cucurbita moschata] >XP_022965179.1 vesicle-asso... [more]
Match NameE-valueIdentityDescription
A0A5A7TC602.5e-11397.22Vesicle-associated protein 2-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3BLE32.5e-11397.22vesicle-associated protein 2-1 OS=Cucumis melo OX=3656 GN=LOC103490849 PE=4 SV=1[more]
A0A0A0L5E32.1e-11295.83MSP domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G017210 PE=4 SV... [more]
A0A6J1HQA23.4e-11093.52vesicle-associated protein 2-1-like OS=Cucurbita maxima OX=3661 GN=LOC111465113 ... [more]
A0A6J1EBL83.4e-11093.52vesicle-associated protein 2-1 OS=Cucurbita moschata OX=3662 GN=LOC111431088 PE=... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 151..178
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 136..155
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 136..151
NoneNo IPR availablePANTHERPTHR10809:SF42VESICLE-ASSOCIATED PROTEIN 2-1coord: 5..213
IPR000535Major sperm protein (MSP) domainPFAMPF00635Motile_Spermcoord: 10..113
e-value: 3.9E-30
score: 103.9
IPR000535Major sperm protein (MSP) domainPROSITEPS50202MSPcoord: 9..129
score: 29.880835
IPR016763Vesicle-associated membrane-protein-associated proteinPIRSFPIRSF019693VAMP_assoc_protcoord: 132..208
e-value: 3.7E-4
score: 18.6
coord: 2..158
e-value: 2.6E-52
score: 176.5
IPR016763Vesicle-associated membrane-protein-associated proteinPANTHERPTHR10809VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEINcoord: 5..213
IPR013783Immunoglobulin-like foldGENE3D2.60.40.10Immunoglobulinscoord: 5..132
e-value: 6.1E-42
score: 144.5
IPR008962PapD-like superfamilySUPERFAMILY49354PapD-likecoord: 5..132

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi11M000062Bhi11M000062mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090158 endoplasmic reticulum membrane organization
biological_process GO:0061817 endoplasmic reticulum-plasma membrane tethering
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane