Bhi10G001608 (gene) Wax gourd (B227) v1

Overview
NameBhi10G001608
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionPectinesterase
Locationchr10: 50011444 .. 50016794 (-)
RNA-Seq ExpressionBhi10G001608
SyntenyBhi10G001608
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGGAAGGGGAAATTATTTTGAAAAATCTTCCAATATATTGGAATGGCTCCTACAGATATGACAAGTGCAAGGCTGATAAGAGTGGATCAATCAGGAAATGGAGATTTTAAAAAGATTCAAGAAGCAATTGATTCTGTTCCATCTCATAACAATGAACTCGTTTTCATTTGGGTTAAGCCTGGAACTTACAGGTTCGTAAAATTAAATCATTTGTTTGATTTCCAATTTTAAATTTTTGTTATGTACTAGTCGACGACTAATACGATTATCAAACTCTTTAACATAGTATAAGTTAACCAACACGAACATAGTTCAATTGATACAAACTTTTTTCTATGATTTTAAGGGTAGGAGGTTTTGTTAAATAAACATTAAAAGAGAGGGTATTTAAAAAAAAAAAAAAAAACTAATGATAAGAAAAATTACACTTTTGGTCTCTTAAGTTTTGAGTTAATAGTAGTTTGTTTTATGAGTTTTCAAACTCCACATTTTAAGCCCCGTTTGGTAACCATTTCGTTTTTTGTTTTTAGTTTTTTTGAAAATTAAGCCTATAGGTACTATTTCCATCTCCTAATTTCTTCTTTTGTTATCCATTTTTACCAATAATTTAAAAGATTAAGTCAAAATTTGAAAATTTAAAAAAGTAGCTTTTTAAAATTTATTTTTATTTTTGAAATTTGACTAAAAGTTCAAGAATTGTAATGCAAATTATTGTACGAATGGGAAATATCTCGACTTAATTTCCAAAAATCAAAAAACAAAAAATAAAATGGTGCACTTTTAACTTTTGAAATTTGATCTCTTTTTCTTAGGGTTTTTGCTACCGTAGCTAATGGAAGTTGACAATTTTTTTTTAATTGAAAAGTATTCTTTTAATTTTTTAAAAATAAAACATATTTTAAACATTCTTGGTCCCTCTTCCTTCTTCTTTCTTCCTGGGTGATTTTGATTTTATTTGAGTTAAATGGTAATCACCAATGGTTCAAAAAATTTTGATTTTAACAATCATGTAGTTCATAGGTTGCTTGTGTATTTTACAATGTACTCAAGGATTTAACTGTAATGATGTAACAAATTGAAGAGGTGTTGGATCAAATTCAAAATTGAAGGGTTGTAATTGTCTCTACTTCAATATTATAAAAAGAATTAGAGATTTTGACTGCTATAATATGGTTGTCTCATGAACTCGAAAAATCTACGTCTCTATTTTCTCTCTGATTTTTTTCTTAGTTTCAATAACATGTAGGATTAGGAGATTCGAACCTCTAACTTAACTTTAAACTTAAATGAGTTGAGCTATTTTCAAACCGGCCTTAACCCTAAATTAATATATAGTCAGAATGATTTTTGGTATTTCTCTTTTCTAAAAATAAAAAAAAAAATTAGTGATAATTTAATTAAAAATAATGATAAAAGAAATTTTTTGTAAGGATGTTCGTGAACTGAGTTGAAACACTTTTTAGATTTAATCTAATTATTTGGGTTATAAGTTTCTTCAACTTAAATAACTCTTATTAAATAATGAAGCTAACCCAACCCGTTCATGAAACATTTTTTGAGTGGGTTGGTTCGGGTTACTCAAATTATTTATTTAATTTTTGTTTTAGAAAATAAGTAAAATATTACTATGTAAAATTTTATTGAATTATTTTCATATATTGGATTAACCTTAACAACTCAATCTAATAAAAATTTTCTTTCCCAGTTGCTACAAAATTATATTTAGCTGTTGGGAAATAAATAAGTTAAAAAATATATATTTTTTAAATTAATAATAAATTTGAGCCCATTTAGGTTATTTTAGACCCATACATAAACTAATTTTGTCTATAAAATTTTCATTTATTTGAACTAAATCTAATCTAAATAGGTTGATAACTAAAACAAAATCTAATCTAAATAGGTTGATAACTAAACTAAAATGTAGTTGTGATTTTTTCGGGTGATTTAGGTTTTTTTTAACGCACTTCATTTTTGGTAATGAAATTGAACAGAGAGAAAATAGTGGTACCGGAGGAGAAGCCATTCATAACAATAAGCGGCAGCAAGGCCTCCGACACCATAATCACATGGAACCAAGGAACTGATTTACTTCAATCTCCGGTCGTCTCCGTCTTTGCCTCCGACTTCATCGGCCGTTTTCTGACCATCCAAAACACCTTCGGCACCACCGGCATTGCGGTGGCGCTAAGGGTCTCTGCAGACAGAGCCGCCTTCTATGGCTGCCGGATCATATCCTTTCAAGATACCCTTCTGGATGACGCCGGAAGACATTACTTCAAGAATTGTTACATTGAAGGAGCCACCGATTTCATCTGCGGAAATGCCGCTTCTCTCTACGAGGTTTTTTCATCTCCTTTAATAATTATTTGATTTTTTAAAAAAATGAAATTGTAAAACATAATTTCTATCTCTGGTTTTTTTAATAATTTAGATAACTATATATTTTAGAGTCCTTTCTCAAAATTTAAAGCTAAGTTGTGAAAACTAATTAATTAAAACTAATATATTGTTTTTAAAAAACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTGGTTGTACATTCATATTTGATTGTATATGAGAAGGTAATAAATTATATTTGTATATTGTAGGAAACTAATTAGGATAACCTCATATTATTTTTAATAGATTAATCAATTAATAATGAAAGAATCATTTTAATTAGATAATTAATTACAAGCATTATTAAAAAAATGAATACACATACTAAGTTAAAATAACTATTAGTGAATCAATTGTTTTTATTTGAAACTAATACTAAAATTAATTAATTAGTATTTGTACATAAATATATTTAACTACATTAATTTAATATATTTTTATTGATAAAAGTGAATTAAATAAGTATATGATTATTTATTTATTACAATCAATTATTTAATTTTAGTTAAAGTAGTGTTCATATTTATTAATTATAAATTTTATTTGTATCAACTTATTTTGCTTAGATAACATGAATAACTTCATTTTAATAAACAATATACAAAAATTAATCTAAGAAAAGAGAACCAAATATGATAGGTTAAGAATGAAAGTTAATAAGAAATATTAGATCAAATGTTTTAAATTAATGATCATATGTTGATTATGAAGGATGAAATTAAAATTAATCTCAATAAATAATTGTTGGATTATAAAATTACAATTAAGTAACCAATTATATTTATTGAAAAATGAATTTTTTACTAAATTTTAAGATATTGTTAGCTAATTAGTCAATGATTTACGTAAATAAGTAATTACATTAATTTAATAAATTTTTATTTATCAAAATAAATTAAGTAAACAATTTAATAGATTATTATTGATTTATTAAAATAAATAATTATATTAATTTATTAAATTAATATTAATTAATTAATTAATAATATTCAAAATGAAATTATATATAAAATAAAAAATAGAAGAAGAGGGAAGAAGAGAAAACCTACACTCTTGGAAGGGAAAATGAAACCTTTGTATGAGAAAGAAGTGTCATAGGTTTTGCATGTCTGTATCAATGTCCATCTAGAAAACTCTTCAACCAAACCAGGTTTTTATTATAAATCGTTGGTTTTGATATAAGTGTACTTTGAACGTGAACTATATTGATTCATGCACTTTTTTCTCTCTTTTTAGAAATATAATTTCAAAACATCTATTTTTGGTCTTTTTGGTCTTATGTTCAATTGTATAGAGGTGTCATTTGCACTCGACGTCGGATAGAGGTGGGGCGATGACGGCGCAACACCGGAACACGACGGAGGAGAACACTGGGTTTGTGTTCTTGGGAGGAAAAATTACAGGAAGTGGTTCCACTTTTCTTGGACGGCCATGGGGGGATTGCTCTAAGGTTGTGTTTGGTTACACCTACATGTCCAATGTTGTTGAACCACAGGGCTGGAATGATTGGGGAGATCAAAGCAAGCAAAGGTAATATATACACCCTAACATTATTTATTCCCATTAGATAAGATTATTAATTAAATTCTCGAGGTTTAGTTTTATTTGTTAGTTTCAGTTTAGTCTATATCATTTTTGAAAGTTACAATTTAGTATTAAAGTGAGCTTGGTTCTAGAGATGAAAGGTTCAATTCTCTATCCCGCAATTATTGAATTAAAAAAAAGTTTTAATTTAGTCTCTACCATTTGAATTTAGTTTTAATCTTTTTCTTATAATTTTAAAAGTTTCGATAATTTAATTCATGTGGTTAAGTTTAAGTTCACATATTCTTATCTCTAAATAGTCTTTTTTAATCACTTCTTTTTACATATTTTTACCGAGATCAAATTAGAGTTAAAACTTAAAACTATATAATAGACTAAATTGAAACTAATTCTATAGAACTCACTAAACTCGAAATACAAACATCAAAATTAATTACAACAAAATAGATATTTCATGACACATGAACAACAGCATATTTAGAGAGGAAGGAAAAAAAACAGTCGTAAAAACTCAATTCACGCGTTGAAACTTTTTAAATCGTAAGAAATCAATAACTCAAATAATAGTTAGGAACTAAATAGAAACTTCCAAAATTTATATGATTCAACATGTGTTATAAATACCAAAGTAAACTTGTGTGGGCAAAAAATATAGGAGAATTTTCTTTTTTTAAAAGTATTAAACATAAAATTTTAGATGCAAAACTCTAAAACTAGAGAAAAATTATGACCAAAAGAAACTGCTGATGTCGAATATTATTACAGTCGTGCATAATTTCTCTGAAATTTGTAACATACTAAGCTTAAAAGTGTTTCCTAGTTCAAATGATTGGAAAACAAAGAAATATAGTAGGGCTTTCTTTTTCTTTTTTTTAATAGTATTTAGAGCCTATCATTTAATTTCTTGTATTTTTAGATGCAATTAAATTCGTTATCCGGTCGTTGTTGATATTTTACCAAAAACCCTACAAGGTGGTAAAATTAATTGTAATTTGCAAACTAAGATTTGTGTGAAACCATTCATATATACAGAACAGTGTTGTATGGAGAATACAAATGCTATGGGCCAGGAGCAAATAGAGCCAAAAGAGTGGCATGGTCACGAAGCCTGTCGACGGATGAAGCTGCAAAACTCTTCACCAAAGACATCATTGGCGGTCGAGCTTGGCTTCGACCTGCACCATCTCATTTCAAAAGGGGCTAGTCCTACCTAGCTCCATCATTATTATTGTCACAATCAACATTCTCTATTAATTTTCTCACATATAATTATAATCTCAATATTACGTATGGATAATATGATGTTTCTTCTTGTTAGTCCGACATTATAATTTTGTTGTCCATGAGTTGGTATCGCAAGACGACACAAAATGATATAACACATTCCCTAAAGATCA

mRNA sequence

TGGAAGGGGAAATTATTTTGAAAAATCTTCCAATATATTGGAATGGCTCCTACAGATATGACAAGTGCAAGGCTGATAAGAGTGGATCAATCAGGAAATGGAGATTTTAAAAAGATTCAAGAAGCAATTGATTCTGTTCCATCTCATAACAATGAACTCGTTTTCATTTGGGTTAAGCCTGGAACTTACAGAGAGAAAATAGTGGTACCGGAGGAGAAGCCATTCATAACAATAAGCGGCAGCAAGGCCTCCGACACCATAATCACATGGAACCAAGGAACTGATTTACTTCAATCTCCGGTCGTCTCCGTCTTTGCCTCCGACTTCATCGGCCGTTTTCTGACCATCCAAAACACCTTCGGCACCACCGGCATTGCGGTGGCGCTAAGGGTCTCTGCAGACAGAGCCGCCTTCTATGGCTGCCGGATCATATCCTTTCAAGATACCCTTCTGGATGACGCCGGAAGACATTACTTCAAGAATTGTTACATTGAAGGAGCCACCGATTTCATCTGCGGAAATGCCGCTTCTCTCTACGAGAGGTGTCATTTGCACTCGACGTCGGATAGAGGTGGGGCGATGACGGCGCAACACCGGAACACGACGGAGGAGAACACTGGGTTTGTGTTCTTGGGAGGAAAAATTACAGGAAGTGGTTCCACTTTTCTTGGACGGCCATGGGGGGATTGCTCTAAGGTTGTGTTTGGTTACACCTACATGTCCAATGTTGTTGAACCACAGGGCTGGAATGATTGGGGAGATCAAAGCAAGCAAAGAACAGTGTTGTATGGAGAATACAAATGCTATGGGCCAGGAGCAAATAGAGCCAAAAGAGTGGCATGGTCACGAAGCCTGTCGACGGATGAAGCTGCAAAACTCTTCACCAAAGACATCATTGGCGGTCGAGCTTGGCTTCGACCTGCACCATCTCATTTCAAAAGGGGCTAGTCCTACCTAGCTCCATCATTATTATTGTCACAATCAACATTCTCTATTAATTTTCTCACATATAATTATAATCTCAATATTACGTATGGATAATATGATGTTTCTTCTTGTTAGTCCGACATTATAATTTTGTTGTCCATGAGTTGGTATCGCAAGACGACACAAAATGATATAACACATTCCCTAAAGATCA

Coding sequence (CDS)

ATGGCTCCTACAGATATGACAAGTGCAAGGCTGATAAGAGTGGATCAATCAGGAAATGGAGATTTTAAAAAGATTCAAGAAGCAATTGATTCTGTTCCATCTCATAACAATGAACTCGTTTTCATTTGGGTTAAGCCTGGAACTTACAGAGAGAAAATAGTGGTACCGGAGGAGAAGCCATTCATAACAATAAGCGGCAGCAAGGCCTCCGACACCATAATCACATGGAACCAAGGAACTGATTTACTTCAATCTCCGGTCGTCTCCGTCTTTGCCTCCGACTTCATCGGCCGTTTTCTGACCATCCAAAACACCTTCGGCACCACCGGCATTGCGGTGGCGCTAAGGGTCTCTGCAGACAGAGCCGCCTTCTATGGCTGCCGGATCATATCCTTTCAAGATACCCTTCTGGATGACGCCGGAAGACATTACTTCAAGAATTGTTACATTGAAGGAGCCACCGATTTCATCTGCGGAAATGCCGCTTCTCTCTACGAGAGGTGTCATTTGCACTCGACGTCGGATAGAGGTGGGGCGATGACGGCGCAACACCGGAACACGACGGAGGAGAACACTGGGTTTGTGTTCTTGGGAGGAAAAATTACAGGAAGTGGTTCCACTTTTCTTGGACGGCCATGGGGGGATTGCTCTAAGGTTGTGTTTGGTTACACCTACATGTCCAATGTTGTTGAACCACAGGGCTGGAATGATTGGGGAGATCAAAGCAAGCAAAGAACAGTGTTGTATGGAGAATACAAATGCTATGGGCCAGGAGCAAATAGAGCCAAAAGAGTGGCATGGTCACGAAGCCTGTCGACGGATGAAGCTGCAAAACTCTTCACCAAAGACATCATTGGCGGTCGAGCTTGGCTTCGACCTGCACCATCTCATTTCAAAAGGGGCTAG

Protein sequence

MAPTDMTSARLIRVDQSGNGDFKKIQEAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKPFITISGSKASDTIITWNQGTDLLQSPVVSVFASDFIGRFLTIQNTFGTTGIAVALRVSADRAAFYGCRIISFQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYERCHLHSTSDRGGAMTAQHRNTTEENTGFVFLGGKITGSGSTFLGRPWGDCSKVVFGYTYMSNVVEPQGWNDWGDQSKQRTVLYGEYKCYGPGANRAKRVAWSRSLSTDEAAKLFTKDIIGGRAWLRPAPSHFKRG
Homology
BLAST of Bhi10G001608 vs. TAIR 10
Match: AT2G21610.1 (pectinesterase 11 )

HSP 1 Score: 439.9 bits (1130), Expect = 1.7e-123
Identity = 201/297 (67.68%), Postives = 244/297 (82.15%), Query Frame = 0

Query: 5   DMTSARLIRVDQSGNGDFKKIQEAIDSVPS--HNNELVFIWVKPGTYREKIVVPEEKPFI 64
           D ++A LIRVDQSG GDF KIQEAI+S+P   +N++L FIWVKPG YREK+V+P EKP+I
Sbjct: 44  DFSTAILIRVDQSGKGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYI 103

Query: 65  TISGSKASDTIITWNQGTDLLQSPVVSVFASDFIGRFLTIQNTFGTTGIAVALRVSADRA 124
           T+SG++AS+T + W+ G D+L+SP +++FASDF+ RFLTIQN FGT G AVALRV+AD+A
Sbjct: 104 TLSGTQASNTFLIWSDGEDILESPTLTIFASDFVCRFLTIQNKFGTAGRAVALRVAADKA 163

Query: 125 AFYGCRIISFQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYERCHLHSTSDRGGAMTA 184
           AFYGC I S+QDTLLDD G HYFKNCYIEGATDFICG+A+SLYERCHLHS S   G++TA
Sbjct: 164 AFYGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHLHSLSPNNGSITA 223

Query: 185 QHRNTTEENTGFVFLGGKITGSGSTFLGRPWGDCSKVVFGYTYMSNVVEPQGWNDWGDQS 244
           Q R +  E +GF FLG K+TGSGSTFLGRPWG  S+VVF Y++ SNVV PQGWN WGD +
Sbjct: 224 QMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFFSNVVAPQGWNQWGDST 283

Query: 245 KQRTVLYGEYKCYGPGANRAKRVAWSRSLSTDEAAKLFTKDIIGGRAWLRPAPSHFK 300
           K+ TV YGEYKCYGPGA+R +RV WS+ LS +EA    +KD IGG+ WLRPAPSHFK
Sbjct: 284 KENTVYYGEYKCYGPGADREQRVEWSKQLSDEEATVFLSKDFIGGKDWLRPAPSHFK 340

BLAST of Bhi10G001608 vs. TAIR 10
Match: AT5G19730.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 277.7 bits (709), Expect = 1.1e-74
Identity = 142/293 (48.46%), Postives = 189/293 (64.51%), Query Frame = 0

Query: 16  QSGNGDFKKIQEAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKPFITISGSKASDTIIT 75
           +S  GDF KIQ+AIDS+P  N   V I V  G Y+EK+ +P  K FITI G  A  T + 
Sbjct: 90  KSNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVE 149

Query: 76  WN---QGTDLLQSPV-------VSVFASDFIGRFLTIQNTF-----GTTG-IAVALRVSA 135
           W    Q  D   +P+        +V +  F+ + +T +NT      G  G  AVALRVSA
Sbjct: 150 WGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSA 209

Query: 136 DRAAFYGCRIISFQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYERCHLHSTSDRGGA 195
           D AAF+GCR++  QDTL D  GRHY+K+CYIEG+ DFI GNA SLYE CH+H+ +D+ GA
Sbjct: 210 DNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGA 269

Query: 196 MTAQHRNTTEENTGFVFLGGKITGSGSTFLGRPWGDCSKVVFGYTYMSNVVEPQGWNDWG 255
           +TAQ R++  E+TGF F+  K+TG+G  +LGR WG  S+VVF YTYM N++ P+GW +WG
Sbjct: 270 VTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWG 329

Query: 256 DQSKQRTVLYGEYKCYGPGANRAKRVAWSRSLSTDEAAKLFTKDIIGGRAWLR 293
           D S++ TV YG+YKC G GAN   RVAW+R L+ +EA    +   I G  W++
Sbjct: 330 DPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFIDGSEWIK 382

BLAST of Bhi10G001608 vs. TAIR 10
Match: AT5G47500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 257.7 bits (657), Expect = 1.2e-68
Identity = 128/301 (42.52%), Postives = 182/301 (60.47%), Query Frame = 0

Query: 10  RLIRVDQSGNGDFKKIQEAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKPFITISGSKA 69
           ++I V  +G+  F+ +Q+A+DS+P +NN+ + I + PG YREK+VVP  KP+IT  G+  
Sbjct: 59  KVITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGR 118

Query: 70  SDTIITW-NQGTDL---------LQSPVVSVFASDFIGRFLTIQNTF-----GTTG-IAV 129
             T I W ++ +DL          Q+  V+V+A+ F  R ++  NT      G  G  AV
Sbjct: 119 DVTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAV 178

Query: 130 ALRVSADRAAFYGCRIISFQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYERCHLHST 189
           A R+S D+A F GC     QDTL DDAGRHYFK CYIEG+ DFI GN  S+Y+ C LHS 
Sbjct: 179 AFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSI 238

Query: 190 SDRGGAMTAQHRNTTEENTGFVFLGGKITGSGSTFLGRPWGDCSKVVFGYTYMSNVVEPQ 249
           + R G++ A  R   EE TGF F+G ++TG+G  ++GR  G  S++V+ YTY   +V   
Sbjct: 239 ASRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHG 298

Query: 250 GWNDWGDQS-KQRTVLYGEYKCYGPGANRAKRVAWSRSLSTDEAAKLFTKDIIGGRAWLR 294
           GW+DW  +S K +T  +G Y CYGPGA   + V+W+R+L  + A     K  + GR W+ 
Sbjct: 299 GWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNGRHWIA 358

BLAST of Bhi10G001608 vs. TAIR 10
Match: AT5G55590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 256.9 bits (655), Expect = 2.0e-68
Identity = 129/300 (43.00%), Postives = 188/300 (62.67%), Query Frame = 0

Query: 10  RLIRVDQSGNGDFKKIQEAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKPFITISG--S 69
           R+I VD++G GD   +Q A+D VP  N++ V I++ PG YREK++VP+ KP+I+  G  S
Sbjct: 80  RVIVVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILPGIYREKVIVPKSKPYISFIGNES 139

Query: 70  KASDTIITW-NQGTDL---------LQSPVVSVFASDFIGRFLTIQNTF----GTTG-IA 129
            A DT+I+W ++ +DL          ++  VS+ +  F    +T +NT     G  G  A
Sbjct: 140 YAGDTVISWSDKASDLGCDGKELGTYRTASVSIESDFFCATAITFENTVVAEAGEQGRQA 199

Query: 130 VALRVSADRAAFYGCRIISFQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYERCHLHS 189
           VALR+  D+A FY  R++  QDTL DD G HYF  CYI+G  DFI GNA SLY+ C +HS
Sbjct: 200 VALRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSLYQDCDIHS 259

Query: 190 TSDRGGAMTAQHRNTTEENTGFVFLGGKITGSGSTFLGRPWGDCSKVVFGYTYMSNVVEP 249
           T+ R GA+ A HR++  E+TGF F+   I+G+G  +LGR WG+ S+ V+   ++++++ P
Sbjct: 260 TAKRYGAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNCFIADIITP 319

Query: 250 QGWNDWGDQSKQRTVLYGEYKCYGPGANRAKRVAWSRSLSTDEAAKLFTKDIIGGRAWLR 293
            GW+DW    +QR V++GEY C G GA R  RV WS++L+ DE      ++ I G  WLR
Sbjct: 320 VGWSDWKHPERQRKVMFGEYNCRGRGAERGGRVPWSKTLTRDEVKPFLGREFIYGDQWLR 379

BLAST of Bhi10G001608 vs. TAIR 10
Match: AT3G29090.1 (pectin methylesterase 31 )

HSP 1 Score: 248.1 bits (632), Expect = 9.3e-66
Identity = 128/307 (41.69%), Postives = 179/307 (58.31%), Query Frame = 0

Query: 6   MTSARLIRVDQSGNGDFKKIQEAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKPFITIS 65
           M + R++RV Q G+GD+  +Q+AIDSVP  N     I + PG YR+ + VP+ K FIT +
Sbjct: 1   MATTRMVRVSQDGSGDYCSVQDAIDSVPLGNTCRTVIRLSPGIYRQPVYVPKRKNFITFA 60

Query: 66  GSKASDTIITWNQ--------------GTDLLQSPVVSVFASDFIGRFLTIQNTFGT-TG 125
           G     T++TWN               GT       V V   DFI   +T +N+    +G
Sbjct: 61  GISPEITVLTWNNTASKIEHHQASRVIGTGTFGCGSVIVEGEDFIAENITFENSAPEGSG 120

Query: 126 IAVALRVSADRAAFYGCRIISFQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYERCHL 185
            AVA+RV+ADR AFY CR + +QDTL    G+ Y K+CYIEG+ DFI GN+ +L E CH+
Sbjct: 121 QAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLEHCHI 180

Query: 186 HSTSDRGGAMTAQHRNTTEENTGFVFLGGKITGSGST---FLGRPWGDCSKVVFGYTYMS 245
           H  S   G +TAQ R +++E+TG+VFL   ITG+G +   +LGRPWG   +VV  YTYM 
Sbjct: 181 HCKSQ--GFITAQSRKSSQESTGYVFLRCVITGNGQSGYMYLGRPWGPFGRVVLAYTYMD 240

Query: 246 NVVEPQGWNDWGDQSKQRTVLYGEYKCYGPGANRAKRVAWSRSLSTDEAAKLFTKDIIG- 292
             +   GW++WG+   +R+  + EY+C+GPG+  ++RV WSR L  DEA        +  
Sbjct: 241 ACIRNVGWHNWGNAENERSACFYEYRCFGPGSCSSERVPWSRELMDDEAGHFVHHSFVDP 300

BLAST of Bhi10G001608 vs. ExPASy Swiss-Prot
Match: Q9SIJ9 (Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 2.3e-122
Identity = 201/297 (67.68%), Postives = 244/297 (82.15%), Query Frame = 0

Query: 5   DMTSARLIRVDQSGNGDFKKIQEAIDSVPS--HNNELVFIWVKPGTYREKIVVPEEKPFI 64
           D ++A LIRVDQSG GDF KIQEAI+S+P   +N++L FIWVKPG YREK+V+P EKP+I
Sbjct: 44  DFSTAILIRVDQSGKGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYI 103

Query: 65  TISGSKASDTIITWNQGTDLLQSPVVSVFASDFIGRFLTIQNTFGTTGIAVALRVSADRA 124
           T+SG++AS+T + W+ G D+L+SP +++FASDF+ RFLTIQN FGT G AVALRV+AD+A
Sbjct: 104 TLSGTQASNTFLIWSDGEDILESPTLTIFASDFVCRFLTIQNKFGTAGRAVALRVAADKA 163

Query: 125 AFYGCRIISFQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYERCHLHSTSDRGGAMTA 184
           AFYGC I S+QDTLLDD G HYFKNCYIEGATDFICG+A+SLYERCHLHS S   G++TA
Sbjct: 164 AFYGCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHLHSLSPNNGSITA 223

Query: 185 QHRNTTEENTGFVFLGGKITGSGSTFLGRPWGDCSKVVFGYTYMSNVVEPQGWNDWGDQS 244
           Q R +  E +GF FLG K+TGSGSTFLGRPWG  S+VVF Y++ SNVV PQGWN WGD +
Sbjct: 224 QMRTSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFFSNVVAPQGWNQWGDST 283

Query: 245 KQRTVLYGEYKCYGPGANRAKRVAWSRSLSTDEAAKLFTKDIIGGRAWLRPAPSHFK 300
           K+ TV YGEYKCYGPGA+R +RV WS+ LS +EA    +KD IGG+ WLRPAPSHFK
Sbjct: 284 KENTVYYGEYKCYGPGADREQRVEWSKQLSDEEATVFLSKDFIGGKDWLRPAPSHFK 340

BLAST of Bhi10G001608 vs. ExPASy Swiss-Prot
Match: Q8VYZ3 (Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 1.5e-73
Identity = 142/293 (48.46%), Postives = 189/293 (64.51%), Query Frame = 0

Query: 16  QSGNGDFKKIQEAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKPFITISGSKASDTIIT 75
           +S  GDF KIQ+AIDS+P  N   V I V  G Y+EK+ +P  K FITI G  A  T + 
Sbjct: 90  KSNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVE 149

Query: 76  WN---QGTDLLQSPV-------VSVFASDFIGRFLTIQNTF-----GTTG-IAVALRVSA 135
           W    Q  D   +P+        +V +  F+ + +T +NT      G  G  AVALRVSA
Sbjct: 150 WGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSA 209

Query: 136 DRAAFYGCRIISFQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYERCHLHSTSDRGGA 195
           D AAF+GCR++  QDTL D  GRHY+K+CYIEG+ DFI GNA SLYE CH+H+ +D+ GA
Sbjct: 210 DNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGA 269

Query: 196 MTAQHRNTTEENTGFVFLGGKITGSGSTFLGRPWGDCSKVVFGYTYMSNVVEPQGWNDWG 255
           +TAQ R++  E+TGF F+  K+TG+G  +LGR WG  S+VVF YTYM N++ P+GW +WG
Sbjct: 270 VTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWG 329

Query: 256 DQSKQRTVLYGEYKCYGPGANRAKRVAWSRSLSTDEAAKLFTKDIIGGRAWLR 293
           D S++ TV YG+YKC G GAN   RVAW+R L+ +EA    +   I G  W++
Sbjct: 330 DPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFIDGSEWIK 382

BLAST of Bhi10G001608 vs. ExPASy Swiss-Prot
Match: Q8LPF3 (Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 1.6e-67
Identity = 128/301 (42.52%), Postives = 182/301 (60.47%), Query Frame = 0

Query: 10  RLIRVDQSGNGDFKKIQEAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKPFITISGSKA 69
           ++I V  +G+  F+ +Q+A+DS+P +NN+ + I + PG YREK+VVP  KP+IT  G+  
Sbjct: 59  KVITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGR 118

Query: 70  SDTIITW-NQGTDL---------LQSPVVSVFASDFIGRFLTIQNTF-----GTTG-IAV 129
             T I W ++ +DL          Q+  V+V+A+ F  R ++  NT      G  G  AV
Sbjct: 119 DVTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAV 178

Query: 130 ALRVSADRAAFYGCRIISFQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYERCHLHST 189
           A R+S D+A F GC     QDTL DDAGRHYFK CYIEG+ DFI GN  S+Y+ C LHS 
Sbjct: 179 AFRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSI 238

Query: 190 SDRGGAMTAQHRNTTEENTGFVFLGGKITGSGSTFLGRPWGDCSKVVFGYTYMSNVVEPQ 249
           + R G++ A  R   EE TGF F+G ++TG+G  ++GR  G  S++V+ YTY   +V   
Sbjct: 239 ASRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHG 298

Query: 250 GWNDWGDQS-KQRTVLYGEYKCYGPGANRAKRVAWSRSLSTDEAAKLFTKDIIGGRAWLR 294
           GW+DW  +S K +T  +G Y CYGPGA   + V+W+R+L  + A     K  + GR W+ 
Sbjct: 299 GWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFVNGRHWIA 358

BLAST of Bhi10G001608 vs. ExPASy Swiss-Prot
Match: Q9FM79 (Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1)

HSP 1 Score: 256.9 bits (655), Expect = 2.8e-67
Identity = 129/300 (43.00%), Postives = 188/300 (62.67%), Query Frame = 0

Query: 10  RLIRVDQSGNGDFKKIQEAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKPFITISG--S 69
           R+I VD++G GD   +Q A+D VP  N++ V I++ PG YREK++VP+ KP+I+  G  S
Sbjct: 80  RVIVVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILPGIYREKVIVPKSKPYISFIGNES 139

Query: 70  KASDTIITW-NQGTDL---------LQSPVVSVFASDFIGRFLTIQNTF----GTTG-IA 129
            A DT+I+W ++ +DL          ++  VS+ +  F    +T +NT     G  G  A
Sbjct: 140 YAGDTVISWSDKASDLGCDGKELGTYRTASVSIESDFFCATAITFENTVVAEAGEQGRQA 199

Query: 130 VALRVSADRAAFYGCRIISFQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYERCHLHS 189
           VALR+  D+A FY  R++  QDTL DD G HYF  CYI+G  DFI GNA SLY+ C +HS
Sbjct: 200 VALRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSLYQDCDIHS 259

Query: 190 TSDRGGAMTAQHRNTTEENTGFVFLGGKITGSGSTFLGRPWGDCSKVVFGYTYMSNVVEP 249
           T+ R GA+ A HR++  E+TGF F+   I+G+G  +LGR WG+ S+ V+   ++++++ P
Sbjct: 260 TAKRYGAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNCFIADIITP 319

Query: 250 QGWNDWGDQSKQRTVLYGEYKCYGPGANRAKRVAWSRSLSTDEAAKLFTKDIIGGRAWLR 293
            GW+DW    +QR V++GEY C G GA R  RV WS++L+ DE      ++ I G  WLR
Sbjct: 320 VGWSDWKHPERQRKVMFGEYNCRGRGAERGGRVPWSKTLTRDEVKPFLGREFIYGDQWLR 379

BLAST of Bhi10G001608 vs. ExPASy Swiss-Prot
Match: Q9LVQ0 (Pectinesterase 31 OS=Arabidopsis thaliana OX=3702 GN=PME31 PE=1 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 1.3e-64
Identity = 128/307 (41.69%), Postives = 179/307 (58.31%), Query Frame = 0

Query: 6   MTSARLIRVDQSGNGDFKKIQEAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKPFITIS 65
           M + R++RV Q G+GD+  +Q+AIDSVP  N     I + PG YR+ + VP+ K FIT +
Sbjct: 1   MATTRMVRVSQDGSGDYCSVQDAIDSVPLGNTCRTVIRLSPGIYRQPVYVPKRKNFITFA 60

Query: 66  GSKASDTIITWNQ--------------GTDLLQSPVVSVFASDFIGRFLTIQNTFGT-TG 125
           G     T++TWN               GT       V V   DFI   +T +N+    +G
Sbjct: 61  GISPEITVLTWNNTASKIEHHQASRVIGTGTFGCGSVIVEGEDFIAENITFENSAPEGSG 120

Query: 126 IAVALRVSADRAAFYGCRIISFQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYERCHL 185
            AVA+RV+ADR AFY CR + +QDTL    G+ Y K+CYIEG+ DFI GN+ +L E CH+
Sbjct: 121 QAVAIRVTADRCAFYNCRFLGWQDTLYLHHGKQYLKDCYIEGSVDFIFGNSTALLEHCHI 180

Query: 186 HSTSDRGGAMTAQHRNTTEENTGFVFLGGKITGSGST---FLGRPWGDCSKVVFGYTYMS 245
           H  S   G +TAQ R +++E+TG+VFL   ITG+G +   +LGRPWG   +VV  YTYM 
Sbjct: 181 HCKSQ--GFITAQSRKSSQESTGYVFLRCVITGNGQSGYMYLGRPWGPFGRVVLAYTYMD 240

Query: 246 NVVEPQGWNDWGDQSKQRTVLYGEYKCYGPGANRAKRVAWSRSLSTDEAAKLFTKDIIG- 292
             +   GW++WG+   +R+  + EY+C+GPG+  ++RV WSR L  DEA        +  
Sbjct: 241 ACIRNVGWHNWGNAENERSACFYEYRCFGPGSCSSERVPWSRELMDDEAGHFVHHSFVDP 300

BLAST of Bhi10G001608 vs. NCBI nr
Match: XP_038902622.1 (putative pectinesterase 11 [Benincasa hispida])

HSP 1 Score: 626.7 bits (1615), Expect = 1.0e-175
Identity = 301/301 (100.00%), Postives = 301/301 (100.00%), Query Frame = 0

Query: 1   MAPTDMTSARLIRVDQSGNGDFKKIQEAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKP 60
           MAPTDMTSARLIRVDQSGNGDFKKIQEAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKP
Sbjct: 1   MAPTDMTSARLIRVDQSGNGDFKKIQEAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKP 60

Query: 61  FITISGSKASDTIITWNQGTDLLQSPVVSVFASDFIGRFLTIQNTFGTTGIAVALRVSAD 120
           FITISGSKASDTIITWNQGTDLLQSPVVSVFASDFIGRFLTIQNTFGTTGIAVALRVSAD
Sbjct: 61  FITISGSKASDTIITWNQGTDLLQSPVVSVFASDFIGRFLTIQNTFGTTGIAVALRVSAD 120

Query: 121 RAAFYGCRIISFQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYERCHLHSTSDRGGAM 180
           RAAFYGCRIISFQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYERCHLHSTSDRGGAM
Sbjct: 121 RAAFYGCRIISFQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYERCHLHSTSDRGGAM 180

Query: 181 TAQHRNTTEENTGFVFLGGKITGSGSTFLGRPWGDCSKVVFGYTYMSNVVEPQGWNDWGD 240
           TAQHRNTTEENTGFVFLGGKITGSGSTFLGRPWGDCSKVVFGYTYMSNVVEPQGWNDWGD
Sbjct: 181 TAQHRNTTEENTGFVFLGGKITGSGSTFLGRPWGDCSKVVFGYTYMSNVVEPQGWNDWGD 240

Query: 241 QSKQRTVLYGEYKCYGPGANRAKRVAWSRSLSTDEAAKLFTKDIIGGRAWLRPAPSHFKR 300
           QSKQRTVLYGEYKCYGPGANRAKRVAWSRSLSTDEAAKLFTKDIIGGRAWLRPAPSHFKR
Sbjct: 241 QSKQRTVLYGEYKCYGPGANRAKRVAWSRSLSTDEAAKLFTKDIIGGRAWLRPAPSHFKR 300

Query: 301 G 302
           G
Sbjct: 301 G 301

BLAST of Bhi10G001608 vs. NCBI nr
Match: XP_008460943.2 (PREDICTED: LOW QUALITY PROTEIN: putative pectinesterase 11 [Cucumis melo])

HSP 1 Score: 574.3 bits (1479), Expect = 6.1e-160
Identity = 272/297 (91.58%), Postives = 283/297 (95.29%), Query Frame = 0

Query: 3   PTDMTSARLIRVDQSGNGDFKKIQEAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKPFI 62
           P D  +ARLIRVD SGNGDFKKIQ+AIDSVPS NNELVFIWVKPGTYREKIVVPE+KP+I
Sbjct: 4   PRDKRTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKPYI 63

Query: 63  TISGSKASDTIITWNQGTDLLQSPVVSVFASDFIGRFLTIQNTFGTTGIAVALRVSADRA 122
           TISGSKASDTIITWNQG DLLQSPVVS+FASDF+GRFLTI+NTFGT+GIAVALRVSADRA
Sbjct: 64  TISGSKASDTIITWNQGRDLLQSPVVSIFASDFVGRFLTIENTFGTSGIAVALRVSADRA 123

Query: 123 AFYGCRIISFQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYERCHLHSTSDRGGAMTA 182
           AFYGCRIISFQDTLLDD+GRHYF NCYIEGATDFICGNAASLYERCHLHSTSDRGGAMTA
Sbjct: 124 AFYGCRIISFQDTLLDDSGRHYFNNCYIEGATDFICGNAASLYERCHLHSTSDRGGAMTA 183

Query: 183 QHRNTTEENTGFVFLGGKITGSGSTFLGRPWGDCSKVVFGYTYMSNVVEPQGWNDWGDQS 242
           QHRNT EENTGFVFLGGKITGSGS FLGRPWGD SKVVFGYTYMSNVVEP+GWNDWGD +
Sbjct: 184 QHRNTAEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGDPT 243

Query: 243 KQRTVLYGEYKCYGPGANRAKRVAWSRSLSTDEAAKLFTKDIIGGRAWLRPAPSHFK 300
           KQRTV YGEYKCYGPGANRAKRV WSRSLSTDEA KLFTKDIIGGRAWLRPAPSHFK
Sbjct: 244 KQRTVSYGEYKCYGPGANRAKRVVWSRSLSTDEATKLFTKDIIGGRAWLRPAPSHFK 300

BLAST of Bhi10G001608 vs. NCBI nr
Match: XP_023533155.1 (putative pectinesterase 11 isoform X3 [Cucurbita pepo subsp. pepo] >XP_023533156.1 putative pectinesterase 11 isoform X4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 573.9 bits (1478), Expect = 7.9e-160
Identity = 266/300 (88.67%), Postives = 286/300 (95.33%), Query Frame = 0

Query: 1   MAPTDMTSARLIRVDQSGNGDFKKIQEAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKP 60
           MAP DM++ARLIRVDQSG GDF+KIQEAIDSVPS NNELVFIW+KPGTYREKIVVPE+KP
Sbjct: 1   MAPVDMSTARLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYREKIVVPEDKP 60

Query: 61  FITISGSKASDTIITWNQGTDLLQSPVVSVFASDFIGRFLTIQNTFGTTGIAVALRVSAD 120
           FIT+SGSKASDTIITWNQG DLLQSP +S+FA+DF+GRFLTIQNT+GTTGIAVALRVSAD
Sbjct: 61  FITLSGSKASDTIITWNQGRDLLQSPAISIFAADFVGRFLTIQNTYGTTGIAVALRVSAD 120

Query: 121 RAAFYGCRIISFQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYERCHLHSTSDRGGAM 180
           RAAFYGCRIISFQDTLLDD G+HYFKNCYIEGATDFICGNAASLYE+CHLHSTS+RGGAM
Sbjct: 121 RAAFYGCRIISFQDTLLDDIGKHYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGGAM 180

Query: 181 TAQHRNTTEENTGFVFLGGKITGSGSTFLGRPWGDCSKVVFGYTYMSNVVEPQGWNDWGD 240
           TAQHRNT  ENTGFVFLG KITGSGSTFLGRPWGDCS+VVFGYT+MSNV+ P+GWNDWGD
Sbjct: 181 TAQHRNTVAENTGFVFLGCKITGSGSTFLGRPWGDCSRVVFGYTHMSNVIVPEGWNDWGD 240

Query: 241 QSKQRTVLYGEYKCYGPGANRAKRVAWSRSLSTDEAAKLFTKDIIGGRAWLRPAPSHFKR 300
            +KQRTV YGEYKCYGPGANRAKRVAWSRSLS DEAAKLFTKD+IGGR WLRPAPSHFKR
Sbjct: 241 HTKQRTVFYGEYKCYGPGANRAKRVAWSRSLSADEAAKLFTKDMIGGRGWLRPAPSHFKR 300

BLAST of Bhi10G001608 vs. NCBI nr
Match: XP_004148862.1 (putative pectinesterase 11 [Cucumis sativus])

HSP 1 Score: 573.9 bits (1478), Expect = 7.9e-160
Identity = 273/301 (90.70%), Postives = 285/301 (94.68%), Query Frame = 0

Query: 1   MAPTDMTSARLIRVDQSGNGDFKKIQEAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKP 60
           M P DMT+ARLIRVD SGNGDFKKIQ+AIDSVPS NNELVFIWVKPGTYREKIVVPE+KP
Sbjct: 12  MGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKP 71

Query: 61  FITISGSKASDTIITWNQGTDLLQSPVVSVFASDFIGRFLTIQNTFGTTGIAVALRVSAD 120
           +ITISGSKASDT ITWNQG DLL+SPVVS+FASDF+GRFLTI+NTFGTTGIAVALRVSAD
Sbjct: 72  YITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVSAD 131

Query: 121 RAAFYGCRIISFQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYERCHLHSTSDRGGAM 180
           RAAFYGCRIISFQDTLLDD GRHYF NCYIEGATDFICGNAASLYE+CHLHSTSDRGGAM
Sbjct: 132 RAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGGAM 191

Query: 181 TAQHRNTTEENTGFVFLGGKITGSGSTFLGRPWGDCSKVVFGYTYMSNVVEPQGWNDWGD 240
           TAQHRNT EENTGFVFLGGKITGSGS FLGRPWGD SKVVFGYTYMSNVVEP+GWNDWGD
Sbjct: 192 TAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGD 251

Query: 241 QSKQRTVLYGEYKCYGPGANRAKRVAWSRSLSTDEAAKLFTKDIIGGRAWLRPAPSHFKR 300
            +KQRTVLYGEYKCYG GANR KRV WSRSLSTDEA+KLFTKDIIGGRAWLRPAPSHFK 
Sbjct: 252 PTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSHFKG 311

Query: 301 G 302
           G
Sbjct: 312 G 312

BLAST of Bhi10G001608 vs. NCBI nr
Match: KGN61801.1 (hypothetical protein Csa_006548 [Cucumis sativus])

HSP 1 Score: 573.9 bits (1478), Expect = 7.9e-160
Identity = 273/301 (90.70%), Postives = 285/301 (94.68%), Query Frame = 0

Query: 1   MAPTDMTSARLIRVDQSGNGDFKKIQEAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKP 60
           M P DMT+ARLIRVD SGNGDFKKIQ+AIDSVPS NNELVFIWVKPGTYREKIVVPE+KP
Sbjct: 3   MGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKP 62

Query: 61  FITISGSKASDTIITWNQGTDLLQSPVVSVFASDFIGRFLTIQNTFGTTGIAVALRVSAD 120
           +ITISGSKASDT ITWNQG DLL+SPVVS+FASDF+GRFLTI+NTFGTTGIAVALRVSAD
Sbjct: 63  YITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVSAD 122

Query: 121 RAAFYGCRIISFQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYERCHLHSTSDRGGAM 180
           RAAFYGCRIISFQDTLLDD GRHYF NCYIEGATDFICGNAASLYE+CHLHSTSDRGGAM
Sbjct: 123 RAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGGAM 182

Query: 181 TAQHRNTTEENTGFVFLGGKITGSGSTFLGRPWGDCSKVVFGYTYMSNVVEPQGWNDWGD 240
           TAQHRNT EENTGFVFLGGKITGSGS FLGRPWGD SKVVFGYTYMSNVVEP+GWNDWGD
Sbjct: 183 TAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGD 242

Query: 241 QSKQRTVLYGEYKCYGPGANRAKRVAWSRSLSTDEAAKLFTKDIIGGRAWLRPAPSHFKR 300
            +KQRTVLYGEYKCYG GANR KRV WSRSLSTDEA+KLFTKDIIGGRAWLRPAPSHFK 
Sbjct: 243 PTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSHFKG 302

Query: 301 G 302
           G
Sbjct: 303 G 303

BLAST of Bhi10G001608 vs. ExPASy TrEMBL
Match: A0A1S3CDL2 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499678 PE=4 SV=1)

HSP 1 Score: 574.3 bits (1479), Expect = 2.9e-160
Identity = 272/297 (91.58%), Postives = 283/297 (95.29%), Query Frame = 0

Query: 3   PTDMTSARLIRVDQSGNGDFKKIQEAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKPFI 62
           P D  +ARLIRVD SGNGDFKKIQ+AIDSVPS NNELVFIWVKPGTYREKIVVPE+KP+I
Sbjct: 4   PRDKRTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKPYI 63

Query: 63  TISGSKASDTIITWNQGTDLLQSPVVSVFASDFIGRFLTIQNTFGTTGIAVALRVSADRA 122
           TISGSKASDTIITWNQG DLLQSPVVS+FASDF+GRFLTI+NTFGT+GIAVALRVSADRA
Sbjct: 64  TISGSKASDTIITWNQGRDLLQSPVVSIFASDFVGRFLTIENTFGTSGIAVALRVSADRA 123

Query: 123 AFYGCRIISFQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYERCHLHSTSDRGGAMTA 182
           AFYGCRIISFQDTLLDD+GRHYF NCYIEGATDFICGNAASLYERCHLHSTSDRGGAMTA
Sbjct: 124 AFYGCRIISFQDTLLDDSGRHYFNNCYIEGATDFICGNAASLYERCHLHSTSDRGGAMTA 183

Query: 183 QHRNTTEENTGFVFLGGKITGSGSTFLGRPWGDCSKVVFGYTYMSNVVEPQGWNDWGDQS 242
           QHRNT EENTGFVFLGGKITGSGS FLGRPWGD SKVVFGYTYMSNVVEP+GWNDWGD +
Sbjct: 184 QHRNTAEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGDPT 243

Query: 243 KQRTVLYGEYKCYGPGANRAKRVAWSRSLSTDEAAKLFTKDIIGGRAWLRPAPSHFK 300
           KQRTV YGEYKCYGPGANRAKRV WSRSLSTDEA KLFTKDIIGGRAWLRPAPSHFK
Sbjct: 244 KQRTVSYGEYKCYGPGANRAKRVVWSRSLSTDEATKLFTKDIIGGRAWLRPAPSHFK 300

BLAST of Bhi10G001608 vs. ExPASy TrEMBL
Match: A0A0A0LP84 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G248110 PE=4 SV=1)

HSP 1 Score: 573.9 bits (1478), Expect = 3.8e-160
Identity = 273/301 (90.70%), Postives = 285/301 (94.68%), Query Frame = 0

Query: 1   MAPTDMTSARLIRVDQSGNGDFKKIQEAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKP 60
           M P DMT+ARLIRVD SGNGDFKKIQ+AIDSVPS NNELVFIWVKPGTYREKIVVPE+KP
Sbjct: 3   MGPGDMTTARLIRVDGSGNGDFKKIQQAIDSVPSQNNELVFIWVKPGTYREKIVVPEDKP 62

Query: 61  FITISGSKASDTIITWNQGTDLLQSPVVSVFASDFIGRFLTIQNTFGTTGIAVALRVSAD 120
           +ITISGSKASDT ITWNQG DLL+SPVVS+FASDF+GRFLTI+NTFGTTGIAVALRVSAD
Sbjct: 63  YITISGSKASDTKITWNQGRDLLESPVVSIFASDFVGRFLTIENTFGTTGIAVALRVSAD 122

Query: 121 RAAFYGCRIISFQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYERCHLHSTSDRGGAM 180
           RAAFYGCRIISFQDTLLDD GRHYF NCYIEGATDFICGNAASLYE+CHLHSTSDRGGAM
Sbjct: 123 RAAFYGCRIISFQDTLLDDTGRHYFNNCYIEGATDFICGNAASLYEKCHLHSTSDRGGAM 182

Query: 181 TAQHRNTTEENTGFVFLGGKITGSGSTFLGRPWGDCSKVVFGYTYMSNVVEPQGWNDWGD 240
           TAQHRNT EENTGFVFLGGKITGSGS FLGRPWGD SKVVFGYTYMSNVVEP+GWNDWGD
Sbjct: 183 TAQHRNTGEENTGFVFLGGKITGSGSMFLGRPWGDFSKVVFGYTYMSNVVEPEGWNDWGD 242

Query: 241 QSKQRTVLYGEYKCYGPGANRAKRVAWSRSLSTDEAAKLFTKDIIGGRAWLRPAPSHFKR 300
            +KQRTVLYGEYKCYG GANR KRV WSRSLSTDEA+KLFTKDIIGGRAWLRPAPSHFK 
Sbjct: 243 PTKQRTVLYGEYKCYGLGANRDKRVVWSRSLSTDEASKLFTKDIIGGRAWLRPAPSHFKG 302

Query: 301 G 302
           G
Sbjct: 303 G 303

BLAST of Bhi10G001608 vs. ExPASy TrEMBL
Match: A0A6J1G7K1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111451478 PE=4 SV=1)

HSP 1 Score: 572.0 bits (1473), Expect = 1.5e-159
Identity = 266/300 (88.67%), Postives = 284/300 (94.67%), Query Frame = 0

Query: 1   MAPTDMTSARLIRVDQSGNGDFKKIQEAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKP 60
           MAP DM++ARLIRVDQSG GDF+KIQEAIDSVPS NNELVFIW+KPGTYREKIVVPE+KP
Sbjct: 1   MAPADMSTARLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYREKIVVPEDKP 60

Query: 61  FITISGSKASDTIITWNQGTDLLQSPVVSVFASDFIGRFLTIQNTFGTTGIAVALRVSAD 120
           FIT+SGSKASDTIITWNQG DLLQSP +S+FA+DF+GRFLTIQNT+GTTGIAVALRVSAD
Sbjct: 61  FITLSGSKASDTIITWNQGRDLLQSPAISIFAADFVGRFLTIQNTYGTTGIAVALRVSAD 120

Query: 121 RAAFYGCRIISFQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYERCHLHSTSDRGGAM 180
           RAAFYGCRIISFQDTLLDD GRHYFKNCYIEGATDFICGNAASLYE+CHLHSTS+RGGAM
Sbjct: 121 RAAFYGCRIISFQDTLLDDVGRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGGAM 180

Query: 181 TAQHRNTTEENTGFVFLGGKITGSGSTFLGRPWGDCSKVVFGYTYMSNVVEPQGWNDWGD 240
           TAQHRNT  ENTGFVFLG KITGSGS  LGRPWGDCS+VVFGYT+MSNVV P+GWNDWGD
Sbjct: 181 TAQHRNTVAENTGFVFLGCKITGSGSALLGRPWGDCSRVVFGYTHMSNVVVPEGWNDWGD 240

Query: 241 QSKQRTVLYGEYKCYGPGANRAKRVAWSRSLSTDEAAKLFTKDIIGGRAWLRPAPSHFKR 300
            +KQRTV YGEYKCYGPGANRAKRV WSRSLSTDEAAKLFTKD+IGGR WLRPAPSHFKR
Sbjct: 241 HTKQRTVFYGEYKCYGPGANRAKRVVWSRSLSTDEAAKLFTKDMIGGRGWLRPAPSHFKR 300

BLAST of Bhi10G001608 vs. ExPASy TrEMBL
Match: A0A6J1KXA2 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111499612 PE=4 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 9.5e-159
Identity = 266/300 (88.67%), Postives = 283/300 (94.33%), Query Frame = 0

Query: 1   MAPTDMTSARLIRVDQSGNGDFKKIQEAIDSVPSHNNELVFIWVKPGTYREKIVVPEEKP 60
           MAP DM++ARLIRVDQSG GDF+KIQEAIDSVP  NNELVFIW+KPGTYREKIVVPE+KP
Sbjct: 1   MAPADMSTARLIRVDQSGKGDFRKIQEAIDSVPPRNNELVFIWIKPGTYREKIVVPEDKP 60

Query: 61  FITISGSKASDTIITWNQGTDLLQSPVVSVFASDFIGRFLTIQNTFGTTGIAVALRVSAD 120
           FIT+SGSKASDTIITWNQG DLLQSP +SVFASDF+ RFLTIQNT+GTTGIAVALRVSAD
Sbjct: 61  FITLSGSKASDTIITWNQGRDLLQSPAISVFASDFVARFLTIQNTYGTTGIAVALRVSAD 120

Query: 121 RAAFYGCRIISFQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYERCHLHSTSDRGGAM 180
           RAAFYGCRIISFQDTLLDD GRHYFKNCYIEGATDFICGNAASLYE+CHLHS S+RGGAM
Sbjct: 121 RAAFYGCRIISFQDTLLDDVGRHYFKNCYIEGATDFICGNAASLYEKCHLHSMSERGGAM 180

Query: 181 TAQHRNTTEENTGFVFLGGKITGSGSTFLGRPWGDCSKVVFGYTYMSNVVEPQGWNDWGD 240
           TAQHRNTT ENTGFVFLG KITGSGS  LGRPWGDCS+VVFGYT+MSNVV P+GWNDWGD
Sbjct: 181 TAQHRNTTAENTGFVFLGCKITGSGSALLGRPWGDCSRVVFGYTHMSNVVVPEGWNDWGD 240

Query: 241 QSKQRTVLYGEYKCYGPGANRAKRVAWSRSLSTDEAAKLFTKDIIGGRAWLRPAPSHFKR 300
            +KQRTV YGEYKCYGPG+NRAKRVAWSRSLSTDEAAKLFTKD+IGGR WLRPAPSHFKR
Sbjct: 241 HTKQRTVFYGEYKCYGPGSNRAKRVAWSRSLSTDEAAKLFTKDMIGGRGWLRPAPSHFKR 300

BLAST of Bhi10G001608 vs. ExPASy TrEMBL
Match: A0A6J1G7A7 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111451478 PE=4 SV=1)

HSP 1 Score: 567.4 bits (1461), Expect = 3.6e-158
Identity = 266/301 (88.37%), Postives = 284/301 (94.35%), Query Frame = 0

Query: 1   MAPTDMTSARLIRVDQSGNGDFKKIQEAIDSVPSHNNELVFIWVKPGTY-REKIVVPEEK 60
           MAP DM++ARLIRVDQSG GDF+KIQEAIDSVPS NNELVFIW+KPGTY REKIVVPE+K
Sbjct: 1   MAPADMSTARLIRVDQSGKGDFRKIQEAIDSVPSRNNELVFIWIKPGTYSREKIVVPEDK 60

Query: 61  PFITISGSKASDTIITWNQGTDLLQSPVVSVFASDFIGRFLTIQNTFGTTGIAVALRVSA 120
           PFIT+SGSKASDTIITWNQG DLLQSP +S+FA+DF+GRFLTIQNT+GTTGIAVALRVSA
Sbjct: 61  PFITLSGSKASDTIITWNQGRDLLQSPAISIFAADFVGRFLTIQNTYGTTGIAVALRVSA 120

Query: 121 DRAAFYGCRIISFQDTLLDDAGRHYFKNCYIEGATDFICGNAASLYERCHLHSTSDRGGA 180
           DRAAFYGCRIISFQDTLLDD GRHYFKNCYIEGATDFICGNAASLYE+CHLHSTS+RGGA
Sbjct: 121 DRAAFYGCRIISFQDTLLDDVGRHYFKNCYIEGATDFICGNAASLYEKCHLHSTSERGGA 180

Query: 181 MTAQHRNTTEENTGFVFLGGKITGSGSTFLGRPWGDCSKVVFGYTYMSNVVEPQGWNDWG 240
           MTAQHRNT  ENTGFVFLG KITGSGS  LGRPWGDCS+VVFGYT+MSNVV P+GWNDWG
Sbjct: 181 MTAQHRNTVAENTGFVFLGCKITGSGSALLGRPWGDCSRVVFGYTHMSNVVVPEGWNDWG 240

Query: 241 DQSKQRTVLYGEYKCYGPGANRAKRVAWSRSLSTDEAAKLFTKDIIGGRAWLRPAPSHFK 300
           D +KQRTV YGEYKCYGPGANRAKRV WSRSLSTDEAAKLFTKD+IGGR WLRPAPSHFK
Sbjct: 241 DHTKQRTVFYGEYKCYGPGANRAKRVVWSRSLSTDEAAKLFTKDMIGGRGWLRPAPSHFK 300

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT2G21610.11.7e-12367.68pectinesterase 11 [more]
AT5G19730.11.1e-7448.46Pectin lyase-like superfamily protein [more]
AT5G47500.11.2e-6842.52Pectin lyase-like superfamily protein [more]
AT5G55590.12.0e-6843.00Pectin lyase-like superfamily protein [more]
AT3G29090.19.3e-6641.69pectin methylesterase 31 [more]
Match NameE-valueIdentityDescription
Q9SIJ92.3e-12267.68Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1[more]
Q8VYZ31.5e-7348.46Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1[more]
Q8LPF31.6e-6742.52Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1[more]
Q9FM792.8e-6743.00Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1[more]
Q9LVQ01.3e-6441.69Pectinesterase 31 OS=Arabidopsis thaliana OX=3702 GN=PME31 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_038902622.11.0e-175100.00putative pectinesterase 11 [Benincasa hispida][more]
XP_008460943.26.1e-16091.58PREDICTED: LOW QUALITY PROTEIN: putative pectinesterase 11 [Cucumis melo][more]
XP_023533155.17.9e-16088.67putative pectinesterase 11 isoform X3 [Cucurbita pepo subsp. pepo] >XP_023533156... [more]
XP_004148862.17.9e-16090.70putative pectinesterase 11 [Cucumis sativus][more]
KGN61801.17.9e-16090.70hypothetical protein Csa_006548 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A1S3CDL22.9e-16091.58Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499678 PE=4 SV=1[more]
A0A0A0LP843.8e-16090.70Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G248110 PE=4 SV=1[more]
A0A6J1G7K11.5e-15988.67Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111451478 PE=4 SV=1[more]
A0A6J1KXA29.5e-15988.67Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111499612 PE=4 SV=1[more]
A0A6J1G7A73.6e-15888.37Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111451478 PE=4 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 6..301
e-value: 6.5E-106
score: 356.3
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 14..282
e-value: 1.0E-67
score: 228.0
NoneNo IPR availablePANTHERPTHR31321:SF77PECTINESTERASE 11-RELATEDcoord: 6..293
NoneNo IPR availablePANTHERPTHR31321ACYL-COA THIOESTER HYDROLASE YBHC-RELATEDcoord: 6..293
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 7..298

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi10M001608Bhi10M001608mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0045490 pectin catabolic process
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity