Bhi10G001319 (gene) Wax gourd (B227) v1

Overview
NameBhi10G001319
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionS-norcoclaurine synthase 1-like
Locationchr10: 41518526 .. 41522721 (-)
RNA-Seq ExpressionBhi10G001319
SyntenyBhi10G001319
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TACAAAATACATGGGAATCATCCATGGTCCATAACCCAACCATGATAAGGAATATTCTTATTCAGAGATGGGTTTGTCCTTGCACTATATAGATCTGATTAGTCCCTCACATTGCTTTTCATCTATCTGTGACCCATTCAACAAAACCGACACAGAAAAATCAAGAAGATGCTGGGTCAACTCCGGCATGAGACGGGGATTCAGGCACCGGCCACCGTGGTATGGCAGCTCTTCGGCAGCCTTGAACTTGGACGACTTGTTGAGAAACGCCTCTCGAATCTTTTCGAGAAAATTGAAGTAGTTGAAGGTGATGGAGGAGAAGGAACTGTTCTTAATCTCATTTTCGCTCCAGGTAATAGTTTCTAATAATCTCTTTTCTGGAAATTCTGTTGATTTAAGGGTTTTTTATTTTAGTTTTACAATGTTTAAATTAAAAGAGCCTTCCTAACTTTTGTGCTCCCACGTATCTACCACATAGAAAAAATTAGAAAAAAAAAATAATTTTTAAAAAATAAAAATATCATGGCAACATTTTATTGAAATTCAAGCGAGCATCCAAGTTTTTCTTCAATTAAAATATTGAGCTTTCTATTTTGCATAATTATTGTCAAAAATACCGACAAAATATCAATGTCAATTTATGTTTTTTTTAAAAAAAAAATTATAAAAAATTTTGAATAAATAAACAAATAATTTTTTTAGAATTTTAATATGATACAAAATTTAAATCTGACTTTTGGGTCAATAATACATACTTTAATGTTAGCAGTAAATAAACATTTAATGTAAATATCATTTATTTTGAATTTCTATTAGTAAATTTTTTTTTAATGTTTTAGATGAATGAAAATATTGGCGTATCGACCAAAATTTAATTCCCTTGTTTATAGACTATTTTGTTGGATCGTTTTCTATTTTTAAAAGTTTAGTTTATACATTATGACATTATGTCATGTGTCTCTTTTTAAGAGTATTATAAAAAAAAAATAATATAATAGACATGAGATGGAATTGAATATTCAATATAAGAGAATAGGTAGATCAGTTAACCGTTGAATGAAAATTATGTGAAAGAAATGGTCATGCTGAGGAAGGATTATAATTTAAGAAATAGTAAATTAAAAATCAATTTCTTATAAAATATGGCTGATTTTGATGTTTTTTTTTTCTTTTCCTTTCCTCTCTATTTCTTTCTTTCTTTCTTTCTTTCTTTCTTTCTCTCTCTTCTTTTTTTTTTTTCTTTTTTAGTTTTTTTGTTTGGTTCACTTGGCAATGAATTTTATTATTTAAGATGTTTTCAAAAGAAAGAAATAGTAAACTAAAAATCAATTTCTTATAAAGTGTGGTTGATTTTGATGTTTTCTTTTTCTTTTCTTTCTTTCTTTCTTTATTTATTAATTTTTTTTGTTTGGTTCACTTGGCAATGAATTTTATTATTTAAGATGTTTTCAGAAGAAAATTAATTTAGGTTTTTATGGGTTATTATAGAAGTTAAAATTTTTAGATAACTTAAAAAAACATGAACCTATCGTGATACAAGCTAAATTATGTGGACGAATCACAAACTAAATTATATGAGTTGAGTTGATCATGAAATTTGATTTAAACACAATTAGATTGTCTCACAATAATTAAGAGGATGTTTGATAATCATTTTATTTTTTATTTTTTTTATTTTCTCAAAATAGATCTGTTTGGTATCTAATTAAGTTTTTTGTTTTTTCAATTTTTTTAAATGGCTGTTTTTTAAATCGATTTTTCATTTTTATAAACGTGCACCCACGTGTAGTATAATTGTCTTAGGGTCTACTACTGGGTAGGCAGATGTTGACTGTGAAGAACTAGGTGTTGGGTTTAATTACCAGAATTTGCAATATACTTTTTAATATTGTTATTTTAATTTAGAAAACTACAACTAGAAATATTCAAAAAAAAATTTGAGGATCCAAAAAATCTGACTAACCAGACGAGTTTGATTGGGTTATCAACTCTTTGTGGGAGAGTGTGTTTTCTGGAAAATGATCCAACATTAATGAAATTTATGTTTTTTTTCTCCAAGCATATTAAAAATAAAAATAAAATTATATTATTTTCTTGTATTTTATTGATATGTTTATTTATGTTTTTTTAATTCATTTGTGATTAATCCACGCGTTCAAAATATTGATAATGTTTTATCAATATGAATTTTTCAAAAAAGAAAGTTATAACAAAGATATAATTCTATGACTATATATATTAACATATTGGATAGAAAATTATTTTTAAAATAACTTGATTTTTATATATAAAATATATAAAACAAAAAAAAAGTCATAAAAAACTTATTTTGATTTTTTTTTAAAACGGAAAACAATCAAACATTTTTTTAGTTTAAAAAACGGAAAATAGAAAACATTAACCAAACATGGTAGTTTTTTTTTTTTAGAACTAAATTATACAAAATATTTCTAAATATTGCGTTCAAAATTTCTTTGAACTTTCAAAAGTTTCAAAAATATCCTTAAATTTTTTTAAAAAAATTAAATATTCTTATTATTAATTTTAGATGGAAATTGTTAATATTTTGTGTAAAAAATATTCCCGAACTTTCAAAAAGTTCTAAAAATACTCCCTTAAATAAAGTTAAAAAATACCCTTATTCTCGATATATGAAAAACCGTTAATACTCTCTTAGTTCTCTATTTTTCCTTTTTCCCTCTCGTCTTCAATATTATTTTACTTCTTTTTTGTTAATTGTTTGATATTTTTTTATCTATAAATAAATGAATAAAATTATCCACTTCAATTAAGATTTATGTACTCCAATAATAATAATAATAATAAATATTTTTCAATATTCCCATTTGCCAATTGCCATGTACTCCAATTGAGAAAGGATTTATGTATATTAAGAAGAGGGTTGGACCCGTTGGTAGTAAATGTTAATTTTAAATGGAGAGTCCTAGAAAAGGGAAAACAAAAACTTTTTTTTCTTTTTTATCGATTTTTTATATTTATTATTATTATTTTTTTTTTTTAAATTAGGCCTACAAAACTATTTCTATCTTTAAATTTGTTTTATTATCTACTTTTAATCAATATTTTTTTTTTAAAAAAAGTCAAATTTTGAAAACTAAAAAATAGTTTTTAAGATTTTTTTTTTTAATTTAACTAAGAATTCAACTATTTTACTTAAGAAATACATGAACTTATAGATGAATATTTAATCTGTAGAATCCCCGATTAGACAAAGAATAAGAGAGTGGGTGAAGAGAAAATTTTATATACACATATATACACTATGCACCGACTTTTACATTGAGATTTACTCTTAGAGTGTGAGTTATATATATATATATGTATGTATGTATGTATGTATTTTAGAAAGTGTGAAAATTAAACATTTTCATCATATCATTAAATACTTTTGTAAAAAATAAAGATCAAGTCATCGAATAATAAAAATTACACTTATTTTGATAAATTTTTTTTTTACCATAAAGATGAAATTGCTACAAATTTTAAAGTATATATCAAGATTAATCTAGAGAACAAGGTCATGTTGTATATATGATTTACTCTAATAACTATAATGTCAAAATGAGCACTCTTCATCTAATATAAAGTATGTGCTATCAATGTTCGACATAAATGCAGGCTTAGGTATGCCAAGTTACAAAGAGAAATTTACCAAAATAGATAATGAGAATCGTATAAAAGAGGCAGAGGTGGTGGAAGGAGGATACTTGGATATTGGGTTTACTCTCTATAGGGTTCGCTTCAAGATAATTGAGAATGGTGAAGATAGCTGCATTGTTGAATCAACAATTGAATATGAAATCATGGAAGAAGCTGCTGCAAATGCCTCACTTGTTACTATACAACCTCTCATTGACATTGCTCAAGAGGCAAATGCATATCTCCTTCAAAACAAGCAGCCTAAGGATGCATAAATGACGAAATCTATGATCATCTTTTATTGGTTTTTATTCCTACCTACAATGTTTCCGACAATAATGATATCATAAAAGGTTAAGAGTACTTGAATTATACTCCATCTATTTGAGTTTAATTTCTTCTGATCATTTATTCATGTTGAATGTAAGAAGATACATTCAAATATATATACATCTAGTTCATGAAAGACATGAAGGGATTGAATCCCACAATGGAAGCGAGTGATTGCTTTGCTTCAATTTGGTTTTAAAATTACA

mRNA sequence

TACAAAATACATGGGAATCATCCATGGTCCATAACCCAACCATGATAAGGAATATTCTTATTCAGAGATGGGTTTGTCCTTGCACTATATAGATCTGATTAGTCCCTCACATTGCTTTTCATCTATCTGTGACCCATTCAACAAAACCGACACAGAAAAATCAAGAAGATGCTGGGTCAACTCCGGCATGAGACGGGGATTCAGGCACCGGCCACCGTGGTATGGCAGCTCTTCGGCAGCCTTGAACTTGGACGACTTGTTGAGAAACGCCTCTCGAATCTTTTCGAGAAAATTGAAGTAGTTGAAGGTGATGGAGGAGAAGGAACTGTTCTTAATCTCATTTTCGCTCCAGGCTTAGGTATGCCAAGTTACAAAGAGAAATTTACCAAAATAGATAATGAGAATCGTATAAAAGAGGCAGAGGTGGTGGAAGGAGGATACTTGGATATTGGGTTTACTCTCTATAGGGTTCGCTTCAAGATAATTGAGAATGGTGAAGATAGCTGCATTGTTGAATCAACAATTGAATATGAAATCATGGAAGAAGCTGCTGCAAATGCCTCACTTGTTACTATACAACCTCTCATTGACATTGCTCAAGAGGCAAATGCATATCTCCTTCAAAACAAGCAGCCTAAGGATGCATAAATGACGAAATCTATGATCATCTTTTATTGGTTTTTATTCCTACCTACAATGTTTCCGACAATAATGATATCATAAAAGGTTAAGAGTACTTGAATTATACTCCATCTATTTGAGTTTAATTTCTTCTGATCATTTATTCATGTTGAATGTAAGAAGATACATTCAAATATATATACATCTAGTTCATGAAAGACATGAAGGGATTGAATCCCACAATGGAAGCGAGTGATTGCTTTGCTTCAATTTGGTTTTAAAATTACA

Coding sequence (CDS)

ATGCTGGGTCAACTCCGGCATGAGACGGGGATTCAGGCACCGGCCACCGTGGTATGGCAGCTCTTCGGCAGCCTTGAACTTGGACGACTTGTTGAGAAACGCCTCTCGAATCTTTTCGAGAAAATTGAAGTAGTTGAAGGTGATGGAGGAGAAGGAACTGTTCTTAATCTCATTTTCGCTCCAGGCTTAGGTATGCCAAGTTACAAAGAGAAATTTACCAAAATAGATAATGAGAATCGTATAAAAGAGGCAGAGGTGGTGGAAGGAGGATACTTGGATATTGGGTTTACTCTCTATAGGGTTCGCTTCAAGATAATTGAGAATGGTGAAGATAGCTGCATTGTTGAATCAACAATTGAATATGAAATCATGGAAGAAGCTGCTGCAAATGCCTCACTTGTTACTATACAACCTCTCATTGACATTGCTCAAGAGGCAAATGCATATCTCCTTCAAAACAAGCAGCCTAAGGATGCATAA

Protein sequence

MLGQLRHETGIQAPATVVWQLFGSLELGRLVEKRLSNLFEKIEVVEGDGGEGTVLNLIFAPGLGMPSYKEKFTKIDNENRIKEAEVVEGGYLDIGFTLYRVRFKIIENGEDSCIVESTIEYEIMEEAAANASLVTIQPLIDIAQEANAYLLQNKQPKDA
Homology
BLAST of Bhi10G001319 vs. TAIR 10
Match: AT5G45860.1 (PYR1-like 11 )

HSP 1 Score: 45.4 bits (106), Expect = 4.8e-05
Identity = 27/110 (24.55%), Postives = 51/110 (46.36%), Query Frame = 0

Query: 11  IQAPATVVWQLFGSLELGRLVEKRLSNLFEK-IEVVEGDGGEGTVLNLIFAPGLGMPSYK 70
           I AP ++VW +     L R    +    F K   +  GDGGEG+V  +    GL     +
Sbjct: 18  IDAPLSLVWSI-----LRRFDNPQAYKQFVKTCNLSSGDGGEGSVREVTVVSGLPAEFSR 77

Query: 71  EKFTKIDNENRIKEAEVVEGGYLDIGFTLYRVRFKIIENGEDSCIVESTI 120
           E+  ++D+E+ +    ++ G +  + +    + F   +  E + +VES +
Sbjct: 78  ERLDELDDESHVMMISIIGGDHRLVNYRSKTMAFVAADTEEKTVVVESYV 122

BLAST of Bhi10G001319 vs. ExPASy Swiss-Prot
Match: A0A3G5BB24 (Norbelladine synthase OS=Narcissus pseudonarcissus OX=39639 GN=NBS PE=1 SV=1)

HSP 1 Score: 147.1 bits (370), Expect = 1.7e-34
Identity = 72/155 (46.45%), Postives = 104/155 (67.10%), Query Frame = 0

Query: 1   MLGQLRHETGIQAPATVVWQLFGSLELGRLVEKRLSNLFEKIEVVEGDGGEGTVLNLIFA 60
           M G L HE  +  PA  +WQ++ +L L +L  + L  +  K+EV EGDGG GT+L + +A
Sbjct: 1   MKGSLSHELEVSLPADQLWQVYSTLRLAQLSAELLPTVISKVEVEEGDGGVGTLLRVTYA 60

Query: 61  PGL-GMPSYKEKFTKIDNENRIKEAEVVEGGYLDIGFTLYRVRFKIIENGEDSCIVESTI 120
            G+ GM  +KE+F KID+E R+KEA  VEGG+LD+GF+ Y +R +I+E G +S +++ST+
Sbjct: 61  LGIPGMKYHKERFVKIDHEKRLKEALFVEGGHLDLGFSSYLIRLEILEKGHNSSVIKSTV 120

Query: 121 EYEIMEEAAANASLVTIQPLIDIAQEANAYLLQNK 155
           EYE+ EE AANAS  T  P + I    + +LLQ K
Sbjct: 121 EYEVDEEHAANASFATTDPFMIIGGAVSEHLLQKK 155

BLAST of Bhi10G001319 vs. ExPASy Swiss-Prot
Match: Q4QTJ1 (S-norcoclaurine synthase 2 OS=Papaver somniferum OX=3469 GN=NCS2 PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.0e-28
Identity = 61/153 (39.87%), Postives = 101/153 (66.01%), Query Frame = 0

Query: 4   QLRHETGIQAPATVVWQLFGSLELGRLV-EKRLSNLFEKIEVVEGDGGEGTVLNLIFAPG 63
           ++R++  +   A  +W ++   ++ RL+ +  L  +F+K++V+EG+GG GTVL+++F PG
Sbjct: 42  EIRYDLEVPTSADSIWSVYSCPDIPRLLRDVLLPGVFQKLDVIEGNGGVGTVLDIVFPPG 101

Query: 64  LGMPSYKEKFTKIDNENRIKEAEVVEGGYLDIGFTLYRVRFKIIENGEDSCIVESTIEYE 123
               SYKEKF  I++E R+KE  ++EGGYLD+G T Y  R  I E   +SC++ES+I YE
Sbjct: 102 AVPRSYKEKFVNINHEKRLKEVIMIEGGYLDMGCTFYMDRIHIFEKTPNSCVIESSIIYE 161

Query: 124 IMEEAAAN-ASLVTIQPLIDIAQEANAYLLQNK 155
           + EE A   A L+T +PL  +A+  + Y+L+ +
Sbjct: 162 VKEEYAGKMAKLITTEPLESMAEVISGYVLKKR 194

BLAST of Bhi10G001319 vs. ExPASy Swiss-Prot
Match: Q4QTJ2 (S-norcoclaurine synthase 1 OS=Papaver somniferum OX=3469 GN=NCS1 PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 1.8e-28
Identity = 63/153 (41.18%), Postives = 100/153 (65.36%), Query Frame = 0

Query: 4   QLRHETGIQAPATVVWQLFGSLELGRLV-EKRLSNLFEKIEVVEGDGGEGTVLNLIFAPG 63
           ++ +ET +Q  A  +W ++ S ++ RL+ +  L  +FEK++V+ G+GG GTVL++ F  G
Sbjct: 42  EITYETEVQTSADSIWNVYSSPDIPRLLRDVLLPGVFEKLDVIAGNGGVGTVLDIAFPLG 101

Query: 64  LGMPSYKEKFTKIDNENRIKEAEVVEGGYLDIGFTLYRVRFKIIENGEDSCIVESTIEYE 123
                YKEKF KI++E R+KE  ++EGGYLD+G T Y  R  I E   +SC++ES+I YE
Sbjct: 102 AVPRRYKEKFVKINHEKRLKEVVMIEGGYLDMGCTFYMDRIHIFEKTPNSCVIESSIIYE 161

Query: 124 IMEEAAAN-ASLVTIQPLIDIAQEANAYLLQNK 155
           + EE A   A L+T +PL  +A+  + Y+L+ +
Sbjct: 162 VKEEYAGKMAKLITTEPLESMAEVISGYVLKKR 194

BLAST of Bhi10G001319 vs. ExPASy Swiss-Prot
Match: Q67A25 (S-norcoclaurine synthase OS=Thalictrum flavum subsp. glaucum OX=150095 PE=1 SV=1)

HSP 1 Score: 114.0 bits (284), Expect = 1.6e-24
Identity = 55/156 (35.26%), Postives = 92/156 (58.97%), Query Frame = 0

Query: 5   LRHETGIQAPATVVWQLFGSLELGRLVEKRLSNLFEKIEVVEGDGGEGTVLNLIFAPGLG 64
           + HE  + A A  +W ++    L + +   L   FEK+E++ GDGG GT+L++ F PG  
Sbjct: 46  IHHELEVAASADDIWTVYSWPGLAKHLPDLLPGAFEKLEII-GDGGVGTILDMTFVPGEF 105

Query: 65  MPSYKEKFTKIDNENRIKEAEVVEGGYLDIGFTLYRVRFKIIENGEDSCIVESTIEYEIM 124
              YKEKF  +DNE+R+K+ +++EGGYLD+G T Y     ++  G+DSC+++S+ EY + 
Sbjct: 106 PHEYKEKFILVDNEHRLKKVQMIEGGYLDLGVTYYMDTIHVVPTGKDSCVIKSSTEYHVK 165

Query: 125 EEAAANAS-LVTIQPLIDIAQEANAYLLQNKQPKDA 160
            E       L+T  PL  +A   +  +L++K   ++
Sbjct: 166 PEFVKIVEPLITTGPLAAMADAISKLVLEHKSKSNS 200

BLAST of Bhi10G001319 vs. ExPASy Swiss-Prot
Match: A2A1A1 (S-norcoclaurine synthase 2 OS=Coptis japonica OX=3442 GN=PR10A PE=2 SV=2)

HSP 1 Score: 102.8 bits (255), Expect = 3.6e-21
Identity = 56/152 (36.84%), Postives = 86/152 (56.58%), Query Frame = 0

Query: 5   LRHETGIQAPATVVWQLFGSLELG-RLVEKRLSNLFEKIEVVEGDGGEGTVLNLIFAPGL 64
           L HE  + A A  VW + GS ELG  L +   + +F K E+  GDGGEG++L++ F PG 
Sbjct: 41  LYHELEVAASADEVWSVEGSPELGLHLPDLLPAGIFAKFEIT-GDGGEGSILDMTFPPGQ 100

Query: 65  GMPSYKEKFTKIDNENRIKEAEVVEGGYLDIGFTLYRVRFKIIENGEDSCIVESTIEYEI 124
               Y+EKF   D++NR K  E ++G + D+G T Y    +++  G DSC+++ST EY +
Sbjct: 101 FPHHYREKFVFFDHKNRYKLVEQIDGDFFDLGVTYYMDTIRVVATGPDSCVIKSTTEYHV 160

Query: 125 MEEAAANAS-LVTIQPLIDIAQEANAYLLQNK 155
             E A     L+   PL  +++     +L+NK
Sbjct: 161 KPEFAKIVKPLIDTVPLAIMSEAIAKVVLENK 191

BLAST of Bhi10G001319 vs. NCBI nr
Match: XP_038901967.1 (norbelladine synthase-like [Benincasa hispida])

HSP 1 Score: 314.3 bits (804), Expect = 6.0e-82
Identity = 159/159 (100.00%), Postives = 159/159 (100.00%), Query Frame = 0

Query: 1   MLGQLRHETGIQAPATVVWQLFGSLELGRLVEKRLSNLFEKIEVVEGDGGEGTVLNLIFA 60
           MLGQLRHETGIQAPATVVWQLFGSLELGRLVEKRLSNLFEKIEVVEGDGGEGTVLNLIFA
Sbjct: 1   MLGQLRHETGIQAPATVVWQLFGSLELGRLVEKRLSNLFEKIEVVEGDGGEGTVLNLIFA 60

Query: 61  PGLGMPSYKEKFTKIDNENRIKEAEVVEGGYLDIGFTLYRVRFKIIENGEDSCIVESTIE 120
           PGLGMPSYKEKFTKIDNENRIKEAEVVEGGYLDIGFTLYRVRFKIIENGEDSCIVESTIE
Sbjct: 61  PGLGMPSYKEKFTKIDNENRIKEAEVVEGGYLDIGFTLYRVRFKIIENGEDSCIVESTIE 120

Query: 121 YEIMEEAAANASLVTIQPLIDIAQEANAYLLQNKQPKDA 160
           YEIMEEAAANASLVTIQPLIDIAQEANAYLLQNKQPKDA
Sbjct: 121 YEIMEEAAANASLVTIQPLIDIAQEANAYLLQNKQPKDA 159

BLAST of Bhi10G001319 vs. NCBI nr
Match: XP_022943440.1 (S-norcoclaurine synthase 1-like [Cucurbita moschata])

HSP 1 Score: 260.4 bits (664), Expect = 1.0e-65
Identity = 134/160 (83.75%), Postives = 141/160 (88.12%), Query Frame = 0

Query: 1   MLGQLRHETGIQAPATVVWQLFGSLELGRLVEKRLSNLFEKIEVVEGDGGEGTVLNLIFA 60
           MLGQL HE  IQAPATV WQL+G LEL RLVE RLSNL +KIEVVEGDGGEGTVLNLIF 
Sbjct: 1   MLGQLSHEAVIQAPATVAWQLYGGLELARLVENRLSNLIQKIEVVEGDGGEGTVLNLIFP 60

Query: 61  PGL-GMPSYKEKFTKIDNENRIKEAEVVEGGYLDIGFTLYRVRFKIIENGEDSCIVESTI 120
           PGL G PSYKEKFTKIDNENRIKE EVVEGG+LDIGFTLYRVR KI+ENG+DSCIVESTI
Sbjct: 61  PGLGGAPSYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIVENGDDSCIVESTI 120

Query: 121 EYEIMEEAAANASLVTIQPLIDIAQEANAYLLQNKQPKDA 160
           EYEI EEAAANASLVT+QPLIDIAQ AN +LL  KQ KDA
Sbjct: 121 EYEIKEEAAANASLVTLQPLIDIAQAANDHLLHYKQLKDA 160

BLAST of Bhi10G001319 vs. NCBI nr
Match: XP_023513283.1 (S-norcoclaurine synthase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 259.6 bits (662), Expect = 1.7e-65
Identity = 133/160 (83.12%), Postives = 141/160 (88.12%), Query Frame = 0

Query: 1   MLGQLRHETGIQAPATVVWQLFGSLELGRLVEKRLSNLFEKIEVVEGDGGEGTVLNLIFA 60
           MLGQL HE  IQAPATV WQL+G LEL RLVE RLSNL +KIEVVEG+GGEGTVLNLIF 
Sbjct: 1   MLGQLSHEAAIQAPATVAWQLYGGLELARLVENRLSNLIQKIEVVEGNGGEGTVLNLIFL 60

Query: 61  PGL-GMPSYKEKFTKIDNENRIKEAEVVEGGYLDIGFTLYRVRFKIIENGEDSCIVESTI 120
           PGL G PSYKEKFTKIDNENRIKE EVVEGG+LDIGFTLYRVR KI+ENG+DSCIVESTI
Sbjct: 61  PGLGGAPSYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIVENGDDSCIVESTI 120

Query: 121 EYEIMEEAAANASLVTIQPLIDIAQEANAYLLQNKQPKDA 160
           EYEI EEAAANASLVT+QPLIDIAQ AN +LL  KQ KDA
Sbjct: 121 EYEIKEEAAANASLVTLQPLIDIAQAANDHLLHYKQLKDA 160

BLAST of Bhi10G001319 vs. NCBI nr
Match: KAA0042962.1 (S-norcoclaurine synthase 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 258.8 bits (660), Expect = 3.0e-65
Identity = 127/157 (80.89%), Postives = 139/157 (88.54%), Query Frame = 0

Query: 1   MLGQLRHETGIQAPATVVWQLFGSLELGRLVEKRLSNLFEKIEVVEGDGGEGTVLNLIFA 60
           MLGQLRHET I  PA V WQLFG LELGR+V ++L NLFEKIE+VEGDGGEGT+ NLIFA
Sbjct: 1   MLGQLRHETVIDVPANVAWQLFGGLELGRIVGEQLPNLFEKIELVEGDGGEGTIFNLIFA 60

Query: 61  PGLGMPSYKEKFTKIDNENRIKEAEVVEGGYLDIGFTLYRVRFKIIENGEDSCIVESTIE 120
           PGLG  +YKEKFTKIDN+NRIKEAE VEGG+L+IGFTLYRVRFKIIENGED CIVE+TIE
Sbjct: 61  PGLGFSNYKEKFTKIDNDNRIKEAETVEGGFLNIGFTLYRVRFKIIENGEDKCIVETTIE 120

Query: 121 YEIMEEAAANASLVTIQPLIDIAQEANAYLLQNKQPK 158
           YEIMEEAAANASLVT+QPLIDI Q A+ YLL NK PK
Sbjct: 121 YEIMEEAAANASLVTLQPLIDIVQVASNYLLHNKNPK 157

BLAST of Bhi10G001319 vs. NCBI nr
Match: XP_004148085.1 (S-norcoclaurine synthase 2 [Cucumis sativus] >KGN63405.1 hypothetical protein Csa_021802 [Cucumis sativus])

HSP 1 Score: 258.8 bits (660), Expect = 3.0e-65
Identity = 128/157 (81.53%), Postives = 140/157 (89.17%), Query Frame = 0

Query: 1   MLGQLRHETGIQAPATVVWQLFGSLELGRLVEKRLSNLFEKIEVVEGDGGEGTVLNLIFA 60
           MLG+L+HE  I  PA V WQLFGSLELGR+V ++L NLFEKIE+VEGDGGEGTVLNLIFA
Sbjct: 1   MLGKLQHEAVIDVPANVTWQLFGSLELGRIVGEQLPNLFEKIELVEGDGGEGTVLNLIFA 60

Query: 61  PGLGMPSYKEKFTKIDNENRIKEAEVVEGGYLDIGFTLYRVRFKIIENGEDSCIVESTIE 120
           PGLG  SYKEKFTKIDNENRIKE E+VEGG+L+IGFTLYRVRFKIIENGED CIVE+TIE
Sbjct: 61  PGLGTSSYKEKFTKIDNENRIKETEIVEGGFLNIGFTLYRVRFKIIENGEDKCIVETTIE 120

Query: 121 YEIMEEAAANASLVTIQPLIDIAQEANAYLLQNKQPK 158
           YEIMEEAAANASLVT+QPLI+I Q AN YLL NK PK
Sbjct: 121 YEIMEEAAANASLVTLQPLIEIVQLANNYLLHNKNPK 157

BLAST of Bhi10G001319 vs. ExPASy TrEMBL
Match: A0A6J1FXT6 (S-norcoclaurine synthase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111448206 PE=3 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 5.0e-66
Identity = 134/160 (83.75%), Postives = 141/160 (88.12%), Query Frame = 0

Query: 1   MLGQLRHETGIQAPATVVWQLFGSLELGRLVEKRLSNLFEKIEVVEGDGGEGTVLNLIFA 60
           MLGQL HE  IQAPATV WQL+G LEL RLVE RLSNL +KIEVVEGDGGEGTVLNLIF 
Sbjct: 1   MLGQLSHEAVIQAPATVAWQLYGGLELARLVENRLSNLIQKIEVVEGDGGEGTVLNLIFP 60

Query: 61  PGL-GMPSYKEKFTKIDNENRIKEAEVVEGGYLDIGFTLYRVRFKIIENGEDSCIVESTI 120
           PGL G PSYKEKFTKIDNENRIKE EVVEGG+LDIGFTLYRVR KI+ENG+DSCIVESTI
Sbjct: 61  PGLGGAPSYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIVENGDDSCIVESTI 120

Query: 121 EYEIMEEAAANASLVTIQPLIDIAQEANAYLLQNKQPKDA 160
           EYEI EEAAANASLVT+QPLIDIAQ AN +LL  KQ KDA
Sbjct: 121 EYEIKEEAAANASLVTLQPLIDIAQAANDHLLHYKQLKDA 160

BLAST of Bhi10G001319 vs. ExPASy TrEMBL
Match: A0A5A7THT1 (S-norcoclaurine synthase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold75G00340 PE=3 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 1.4e-65
Identity = 127/157 (80.89%), Postives = 139/157 (88.54%), Query Frame = 0

Query: 1   MLGQLRHETGIQAPATVVWQLFGSLELGRLVEKRLSNLFEKIEVVEGDGGEGTVLNLIFA 60
           MLGQLRHET I  PA V WQLFG LELGR+V ++L NLFEKIE+VEGDGGEGT+ NLIFA
Sbjct: 1   MLGQLRHETVIDVPANVAWQLFGGLELGRIVGEQLPNLFEKIELVEGDGGEGTIFNLIFA 60

Query: 61  PGLGMPSYKEKFTKIDNENRIKEAEVVEGGYLDIGFTLYRVRFKIIENGEDSCIVESTIE 120
           PGLG  +YKEKFTKIDN+NRIKEAE VEGG+L+IGFTLYRVRFKIIENGED CIVE+TIE
Sbjct: 61  PGLGFSNYKEKFTKIDNDNRIKEAETVEGGFLNIGFTLYRVRFKIIENGEDKCIVETTIE 120

Query: 121 YEIMEEAAANASLVTIQPLIDIAQEANAYLLQNKQPK 158
           YEIMEEAAANASLVT+QPLIDI Q A+ YLL NK PK
Sbjct: 121 YEIMEEAAANASLVTLQPLIDIVQVASNYLLHNKNPK 157

BLAST of Bhi10G001319 vs. ExPASy TrEMBL
Match: A0A0A0LNC6 (Bet_v_1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G439140 PE=3 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 1.4e-65
Identity = 128/157 (81.53%), Postives = 140/157 (89.17%), Query Frame = 0

Query: 1   MLGQLRHETGIQAPATVVWQLFGSLELGRLVEKRLSNLFEKIEVVEGDGGEGTVLNLIFA 60
           MLG+L+HE  I  PA V WQLFGSLELGR+V ++L NLFEKIE+VEGDGGEGTVLNLIFA
Sbjct: 1   MLGKLQHEAVIDVPANVTWQLFGSLELGRIVGEQLPNLFEKIELVEGDGGEGTVLNLIFA 60

Query: 61  PGLGMPSYKEKFTKIDNENRIKEAEVVEGGYLDIGFTLYRVRFKIIENGEDSCIVESTIE 120
           PGLG  SYKEKFTKIDNENRIKE E+VEGG+L+IGFTLYRVRFKIIENGED CIVE+TIE
Sbjct: 61  PGLGTSSYKEKFTKIDNENRIKETEIVEGGFLNIGFTLYRVRFKIIENGEDKCIVETTIE 120

Query: 121 YEIMEEAAANASLVTIQPLIDIAQEANAYLLQNKQPK 158
           YEIMEEAAANASLVT+QPLI+I Q AN YLL NK PK
Sbjct: 121 YEIMEEAAANASLVTLQPLIEIVQLANNYLLHNKNPK 157

BLAST of Bhi10G001319 vs. ExPASy TrEMBL
Match: A0A6J1J835 (S-norcoclaurine synthase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111484311 PE=3 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 1.9e-65
Identity = 133/160 (83.12%), Postives = 140/160 (87.50%), Query Frame = 0

Query: 1   MLGQLRHETGIQAPATVVWQLFGSLELGRLVEKRLSNLFEKIEVVEGDGGEGTVLNLIFA 60
           MLGQL HE  IQAPATV WQL+G LEL RLVE RLSNL +KIEVVEGDGGEGTVLNLIF 
Sbjct: 1   MLGQLSHEAAIQAPATVAWQLYGGLELARLVENRLSNLIQKIEVVEGDGGEGTVLNLIFP 60

Query: 61  PGL-GMPSYKEKFTKIDNENRIKEAEVVEGGYLDIGFTLYRVRFKIIENGEDSCIVESTI 120
           PGL G  SYKEKFTKIDNENRIKE EVVEGG+LDIGFTLYRVR KI+ENG+DSCIVESTI
Sbjct: 61  PGLGGASSYKEKFTKIDNENRIKETEVVEGGFLDIGFTLYRVRLKIVENGDDSCIVESTI 120

Query: 121 EYEIMEEAAANASLVTIQPLIDIAQEANAYLLQNKQPKDA 160
           EYEI EEAAANASLVT+QPLIDIAQ AN +LL  KQ KDA
Sbjct: 121 EYEIKEEAAANASLVTLQPLIDIAQAANDHLLHYKQLKDA 160

BLAST of Bhi10G001319 vs. ExPASy TrEMBL
Match: A0A1S3CA50 (S-norcoclaurine synthase 2-like OS=Cucumis melo OX=3656 GN=LOC103498383 PE=3 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 1.9e-65
Identity = 126/157 (80.25%), Postives = 139/157 (88.54%), Query Frame = 0

Query: 1   MLGQLRHETGIQAPATVVWQLFGSLELGRLVEKRLSNLFEKIEVVEGDGGEGTVLNLIFA 60
           MLGQLRHET I  PA V WQLFG LELGR+V ++L NLFEKIE+VEGDGGEGT+ NLIFA
Sbjct: 1   MLGQLRHETVIDVPANVAWQLFGGLELGRIVGEQLPNLFEKIELVEGDGGEGTIFNLIFA 60

Query: 61  PGLGMPSYKEKFTKIDNENRIKEAEVVEGGYLDIGFTLYRVRFKIIENGEDSCIVESTIE 120
           PGLG  +YKEKFTKIDN+NRIKEAE VEGG+L+IGFTLYRVRFKIIENGED CIVE+T+E
Sbjct: 61  PGLGFSNYKEKFTKIDNDNRIKEAETVEGGFLNIGFTLYRVRFKIIENGEDKCIVETTVE 120

Query: 121 YEIMEEAAANASLVTIQPLIDIAQEANAYLLQNKQPK 158
           YEIMEEAAANASLVT+QPLIDI Q A+ YLL NK PK
Sbjct: 121 YEIMEEAAANASLVTLQPLIDIVQVASNYLLHNKNPK 157

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G45860.14.8e-0524.55PYR1-like 11 [more]
Match NameE-valueIdentityDescription
A0A3G5BB241.7e-3446.45Norbelladine synthase OS=Narcissus pseudonarcissus OX=39639 GN=NBS PE=1 SV=1[more]
Q4QTJ11.0e-2839.87S-norcoclaurine synthase 2 OS=Papaver somniferum OX=3469 GN=NCS2 PE=1 SV=1[more]
Q4QTJ21.8e-2841.18S-norcoclaurine synthase 1 OS=Papaver somniferum OX=3469 GN=NCS1 PE=1 SV=1[more]
Q67A251.6e-2435.26S-norcoclaurine synthase OS=Thalictrum flavum subsp. glaucum OX=150095 PE=1 SV=1[more]
A2A1A13.6e-2136.84S-norcoclaurine synthase 2 OS=Coptis japonica OX=3442 GN=PR10A PE=2 SV=2[more]
Match NameE-valueIdentityDescription
XP_038901967.16.0e-82100.00norbelladine synthase-like [Benincasa hispida][more]
XP_022943440.11.0e-6583.75S-norcoclaurine synthase 1-like [Cucurbita moschata][more]
XP_023513283.11.7e-6583.13S-norcoclaurine synthase 1-like [Cucurbita pepo subsp. pepo][more]
KAA0042962.13.0e-6580.89S-norcoclaurine synthase 2-like [Cucumis melo var. makuwa][more]
XP_004148085.13.0e-6581.53S-norcoclaurine synthase 2 [Cucumis sativus] >KGN63405.1 hypothetical protein Cs... [more]
Match NameE-valueIdentityDescription
A0A6J1FXT65.0e-6683.75S-norcoclaurine synthase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111448206 PE... [more]
A0A5A7THT11.4e-6580.89S-norcoclaurine synthase 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A0A0LNC61.4e-6581.53Bet_v_1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G439140 PE=... [more]
A0A6J1J8351.9e-6583.13S-norcoclaurine synthase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111484311 PE=3... [more]
A0A1S3CA501.9e-6580.25S-norcoclaurine synthase 2-like OS=Cucumis melo OX=3656 GN=LOC103498383 PE=3 SV=... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000916Bet v I/Major latex proteinPFAMPF00407Bet_v_1coord: 3..152
e-value: 5.0E-14
score: 52.5
IPR023393START-like domain superfamilyGENE3D3.30.530.20coord: 1..155
e-value: 3.9E-41
score: 142.6
NoneNo IPR availablePANTHERPTHR31213FAMILY NOT NAMEDcoord: 3..155
NoneNo IPR availablePANTHERPTHR31213:SF145S-NORCOCLAURINE SYNTHASE 2-LIKEcoord: 3..155
NoneNo IPR availableCDDcd07816Bet_v1-likecoord: 3..127
e-value: 5.8294E-33
score: 112.283
NoneNo IPR availableSUPERFAMILY55961Bet v1-likecoord: 4..153

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi10M001319Bhi10M001319mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009738 abscisic acid-activated signaling pathway
biological_process GO:0006952 defense response
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0080163 regulation of protein serine/threonine phosphatase activity
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0010427 abscisic acid binding
molecular_function GO:0004864 protein phosphatase inhibitor activity
molecular_function GO:0038023 signaling receptor activity