Bhi10G001172 (gene) Wax gourd (B227) v1

Overview


Name	Bhi10G001172
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	cytochrome P450 81D1-like
Location	chr10: 36039820 .. 36040431 (+)
RNA-Seq Expression	Bhi10G001172
Synteny	Bhi10G001172

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATCATATTTATAGCTGGGATTGACACAACAGCCGTAACCTTAGAATGGGCACTCTCTCATCTACTAAATAATCCTAATGTATTGGAGAAAGCAAAAGTTGAGATAGACAGTGCCATTGGACAAGAGCGACTAATAACTGAAGCCGATTTGCCAAGTTTGAACTATATACAAGGAATAATTTCCGAAACACTTCGATTGACCCCTGCAGCTCCTTTGCTTGTTCCCCATTGCGCTTCCGAAGATTGTAAAATTGGAGCCTACGATGTACCACGTGACACAATGGTATTGATTAATGCTTGGGCTATTCATAGAGATCCCAAATTATGGGAGAATGCCACAAAATTTAAGCCTGAAAGACATACAAATCCTATTGGAACTGACTCATATAAATTTTTACCATTTGGATTGGGAAGGAGAGCCTGTCCTGGAATAGGAATAGCCCAACGGATGGCTACCTTGACTTTGGCGACGATGATTCAATGCTTTGAGTGGGAAAGAGAGGGCTCTTCATTGGTTGATATGAGTGAAGGTGAAGGGATCACTATGCCCAAAGCTCGACGATTGGTAGCCAAGTGCAAACCACGTCCAATCATGAAAGCTATATTTAGCTAA

mRNA sequence

ATCATATTTATAGCTGGGATTGACACAACAGCCGTAACCTTAGAATGGGCACTCTCTCATCTACTAAATAATCCTAATGTATTGGAGAAAGCAAAAGTTGAGATAGACAGTGCCATTGGACAAGAGCGACTAATAACTGAAGCCGATTTGCCAAGTTTGAACTATATACAAGGAATAATTTCCGAAACACTTCGATTGACCCCTGCAGCTCCTTTGCTTGTTCCCCATTGCGCTTCCGAAGATTGTAAAATTGGAGCCTACGATGTACCACGTGACACAATGGTATTGATTAATGCTTGGGCTATTCATAGAGATCCCAAATTATGGGAGAATGCCACAAAATTTAAGCCTGAAAGACATACAAATCCTATTGGAACTGACTCATATAAATTTTTACCATTTGGATTGGGAAGGAGAGCCTGTCCTGGAATAGGAATAGCCCAACGGATGGCTACCTTGACTTTGGCGACGATGATTCAATGCTTTGAGTGGGAAAGAGAGGGCTCTTCATTGGTTGATATGAGTGAAGGTGAAGGGATCACTATGCCCAAAGCTCGACGATTGGTAGCCAAGTGCAAACCACGTCCAATCATGAAAGCTATATTTAGCTAA

Coding sequence (CDS)

ATCATATTTATAGCTGGGATTGACACAACAGCCGTAACCTTAGAATGGGCACTCTCTCATCTACTAAATAATCCTAATGTATTGGAGAAAGCAAAAGTTGAGATAGACAGTGCCATTGGACAAGAGCGACTAATAACTGAAGCCGATTTGCCAAGTTTGAACTATATACAAGGAATAATTTCCGAAACACTTCGATTGACCCCTGCAGCTCCTTTGCTTGTTCCCCATTGCGCTTCCGAAGATTGTAAAATTGGAGCCTACGATGTACCACGTGACACAATGGTATTGATTAATGCTTGGGCTATTCATAGAGATCCCAAATTATGGGAGAATGCCACAAAATTTAAGCCTGAAAGACATACAAATCCTATTGGAACTGACTCATATAAATTTTTACCATTTGGATTGGGAAGGAGAGCCTGTCCTGGAATAGGAATAGCCCAACGGATGGCTACCTTGACTTTGGCGACGATGATTCAATGCTTTGAGTGGGAAAGAGAGGGCTCTTCATTGGTTGATATGAGTGAAGGTGAAGGGATCACTATGCCCAAAGCTCGACGATTGGTAGCCAAGTGCAAACCACGTCCAATCATGAAAGCTATATTTAGCTAA

Protein sequence

IIFIAGIDTTAVTLEWALSHLLNNPNVLEKAKVEIDSAIGQERLITEADLPSLNYIQGIISETLRLTPAAPLLVPHCASEDCKIGAYDVPRDTMVLINAWAIHRDPKLWENATKFKPERHTNPIGTDSYKFLPFGLGRRACPGIGIAQRMATLTLATMIQCFEWEREGSSLVDMSEGEGITMPKARRLVAKCKPRPIMKAIFS

Homology

[Close]

BLAST of Bhi10G001172 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 263.5 bits (672), Expect = 1.4e-70
Identity = 124/200 (62.00%), Postives = 153/200 (76.50%), Query Frame = 0


Query: 2   IFIAGIDTTAVTLEWALSHLLNNPNVLEKAKVEIDSAIGQERLITEADLPSLNYIQGIIS 61
           +   G DTTAVTLEWALS LLNNP VL KA+ EID  IG +RL+ E+D+P+L Y+Q I+S
Sbjct: 297 LIAGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQNIVS 356

Query: 62  ETLRLTPAAPLLVPHCASEDCKIGAYDVPRDTMVLINAWAIHRDPKLWENATKFKPERHT 121
           ETLRL PAAP+L+PH AS+DCK+G YD+PR TM+L NAWAIHRDP LW++ T FKPER  
Sbjct: 357 ETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERFE 416

Query: 122 NPIGTDSYKFLPFGLGRRACPGIGIAQRMATLTLATMIQCFEWEREGSSLVDMSEGEGIT 181
                ++ K +PFGLGRRACPG G+AQR+ TL+L ++IQCFEWER G   VDM+EG G+T
Sbjct: 417 KE--GEAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEVDMTEGPGLT 476

Query: 182 MPKARRLVAKCKPRPIMKAI 202
           MPKAR L A C+ R  +  I
Sbjct: 477 MPKARPLEAMCRARDFVGKI 494

[Close]

BLAST of Bhi10G001172 vs. TAIR 10
Match: AT1G66540.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 255.4 bits (651), Expect = 3.9e-68
Identity = 118/200 (59.00%), Postives = 154/200 (77.00%), Query Frame = 0


Query: 2   IFIAGIDTTAVTLEWALSHLLNNPNVLEKAKVEIDSAIGQERLITEADLPSLNYIQGIIS 61
           + +AG DT+AVTLEWALS LLNNP VL+K + EID+ IG +RL+ E+D+P+L Y+Q I+S
Sbjct: 184 LILAGTDTSAVTLEWALSSLLNNPEVLKKVRDEIDNQIGLDRLLEESDIPNLPYLQNIVS 243

Query: 62  ETLRLTPAAPLLVPHCASEDCKIGAYDVPRDTMVLINAWAIHRDPKLWENATKFKPERHT 121
           ETLRL PA PLLVPH +SEDCK+G YD+P  TM+L+N WAIHRDP+LW++   FKPER  
Sbjct: 244 ETLRLYPAGPLLVPHISSEDCKVGGYDMPCGTMLLVNVWAIHRDPRLWDDPASFKPERFE 303

Query: 122 NPIGTDSYKFLPFGLGRRACPGIGIAQRMATLTLATMIQCFEWEREGSSLVDMSEGEGIT 181
                +++K L FGLGRRACPG G+A+R+ +L+L ++IQCFEWER G   VDM+EG G+T
Sbjct: 304 KE--GETHKLLTFGLGRRACPGSGLARRLVSLSLGSLIQCFEWERIGEEEVDMTEGGGLT 363

Query: 182 MPKARRLVAKCKPRPIMKAI 202
           MP+A  LVA C+ R  +  I
Sbjct: 364 MPRAIPLVAMCRARAFVGKI 381

[Close]

BLAST of Bhi10G001172 vs. TAIR 10
Match: AT1G66540.2 (Cytochrome P450 superfamily protein )

HSP 1 Score: 255.4 bits (651), Expect = 3.9e-68
Identity = 118/200 (59.00%), Postives = 154/200 (77.00%), Query Frame = 0


Query: 2   IFIAGIDTTAVTLEWALSHLLNNPNVLEKAKVEIDSAIGQERLITEADLPSLNYIQGIIS 61
           + +AG DT+AVTLEWALS LLNNP VL+K + EID+ IG +RL+ E+D+P+L Y+Q I+S
Sbjct: 125 LILAGTDTSAVTLEWALSSLLNNPEVLKKVRDEIDNQIGLDRLLEESDIPNLPYLQNIVS 184

Query: 62  ETLRLTPAAPLLVPHCASEDCKIGAYDVPRDTMVLINAWAIHRDPKLWENATKFKPERHT 121
           ETLRL PA PLLVPH +SEDCK+G YD+P  TM+L+N WAIHRDP+LW++   FKPER  
Sbjct: 185 ETLRLYPAGPLLVPHISSEDCKVGGYDMPCGTMLLVNVWAIHRDPRLWDDPASFKPERFE 244

Query: 122 NPIGTDSYKFLPFGLGRRACPGIGIAQRMATLTLATMIQCFEWEREGSSLVDMSEGEGIT 181
                +++K L FGLGRRACPG G+A+R+ +L+L ++IQCFEWER G   VDM+EG G+T
Sbjct: 245 KE--GETHKLLTFGLGRRACPGSGLARRLVSLSLGSLIQCFEWERIGEEEVDMTEGGGLT 304

Query: 182 MPKARRLVAKCKPRPIMKAI 202
           MP+A  LVA C+ R  +  I
Sbjct: 305 MPRAIPLVAMCRARAFVGKI 322

[Close]

BLAST of Bhi10G001172 vs. TAIR 10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )

HSP 1 Score: 254.2 bits (648), Expect = 8.7e-68
Identity = 121/203 (59.61%), Postives = 154/203 (75.86%), Query Frame = 0


Query: 2   IFIAGIDTTAVTLEWALSHLLNNPNVLEKAKVEIDSAIGQERLITEADLPSLNYIQGIIS 61
           + +AG DT+AVTLEW LS LLN+P +L KA+ EID+ +G  RL+ E+DL  L Y+Q I+S
Sbjct: 297 LILAGTDTSAVTLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEESDLSHLPYLQNIVS 356

Query: 62  ETLRLTPAAPLLVPHCASEDCKIGAYDVPRDTMVLINAWAIHRDPKLWENATKFKPERHT 121
           E+LRL PA+PLLVPH ASEDCK+G Y +PR TM+L NAWAIHRDPK+W++ T FKPER  
Sbjct: 357 ESLRLYPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTSFKPERFE 416

Query: 122 NPIGTDSYKFLPFGLGRRACPGIGIAQRMATLTLATMIQCFEWEREGSSLVDMSE-GEGI 181
                ++ K L FGLGRRACPG G+AQR+A+LT+ ++IQCFEWER G   VDM+E G G+
Sbjct: 417 KE--GEAQKLLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEVDMTEGGGGV 476

Query: 182 TMPKARRLVAKCKPRPIMKAIFS 204
            MPKA  LVA CK RP++  I +
Sbjct: 477 IMPKAIPLVAMCKARPVVGKILN 497

[Close]

BLAST of Bhi10G001172 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 251.5 bits (641), Expect = 5.7e-67
Identity = 117/202 (57.92%), Postives = 155/202 (76.73%), Query Frame = 0


Query: 2   IFIAGIDTTAVTLEWALSHLLNNPNVLEKAKVEIDSAIGQERLITEADLPSLNYIQGIIS 61
           + +AG DT+AVTLEWALS++LN+P+VL KA+ EID  IG +RL+ E+D+ +L Y+Q I+S
Sbjct: 296 LILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVS 355

Query: 62  ETLRLTPAAPLLVPHCASEDCKIGAYDVPRDTMVLINAWAIHRDPKLWENATKFKPERHT 121
           ETLRL PAAP+L+PH ASEDCK+  YD+PR T++L N WAIHRDP+LW++   FKPER  
Sbjct: 356 ETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFE 415

Query: 122 NPIGTDSYKFLPFGLGRRACPGIGIAQRMATLTLATMIQCFEWEREGSSLVDMSEGEGIT 181
                ++ K +PFGLGRRACPG G+A R+  LTL ++IQC EWE+ G   VDMSEG+G+T
Sbjct: 416 KE--GEAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGEE-VDMSEGKGVT 475

Query: 182 MPKARRLVAKCKPRPIMKAIFS 204
           MPKA+ L A C+ RP +  IF+
Sbjct: 476 MPKAKPLEAMCRARPSVVKIFN 494

[Close]

BLAST of Bhi10G001172 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 5.0e-68
Identity = 116/203 (57.14%), Postives = 154/203 (75.86%), Query Frame = 0


Query: 1   IIFIAGIDTTAVTLEWALSHLLNNPNVLEKAKVEIDSAIGQERLITEADLPSLNYIQGII 60
           ++ +AG DT+AVT+EWA+S LLN+P  LEKA+ EI++ +G  RLI E DLP L Y+  II
Sbjct: 295 VLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNII 354

Query: 61  SETLRLTPAAPLLVPHCASEDCKIGAYDVPRDTMVLINAWAIHRDPKLWENATKFKPERH 120
           SET RL PAAP+LVPH +S+DCK+  YDVP+ T++L+NAWAIHRDP+ W+  T FKPERH
Sbjct: 355 SETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPERH 414

Query: 121 TNPIGTDSYKFLPFGLGRRACPGIGIAQRMATLTLATMIQCFEWEREGSSLVDMSEGEGI 180
              +  +  K +PFG+GRR+CPG G+AQR+  LTL  +IQCFEW+R G + +DM+EG G+
Sbjct: 415 -GGVELEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSGL 474

Query: 181 TMPKARRLVAKCKPRPIMKAIFS 204
           TMPKA+ L A CKPR I+  + S
Sbjct: 475 TMPKAQPLEALCKPRNILHKVVS 496

[Close]

BLAST of Bhi10G001172 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 1.8e-65
Identity = 122/203 (60.10%), Postives = 151/203 (74.38%), Query Frame = 0


Query: 1   IIFIAGIDTTAVTLEWALSHLLNNPNVLEKAKVEIDSAIGQERLITEADLPSLNYIQGII 60
           I+ IAG +T+AVTLEWALS+LLN+P+V+ KA+ EID+ +G +RLI EADL  L Y++ I+
Sbjct: 302 IMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIV 361

Query: 61  SETLRLTPAAPLLVPHCASEDCKIGAYDVPRDTMVLINAWAIHRDPKLWENATKFKPERH 120
            ETLRL PA PLLVPH ASEDCKIG+YD+PR T +L+NAWAIHRDP  W++   FKPER 
Sbjct: 362 LETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERF 421

Query: 121 TNPIGTDSYKFLPFGLGRRACPGIGIAQRMATLTLATMIQCFEWEREGSSLVDMSEGEGI 180
                 ++ K L FGLGRRACPG G+AQR+  L L ++IQCFEWER G+  VDM EG G 
Sbjct: 422 EKE--EEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGN 481

Query: 181 TMPKARRLVAKCKPRPIMKAIFS 204
           T+PKA  L A CK RP +  I S
Sbjct: 482 TVPKAIPLKAICKARPFLHKIIS 502

[Close]

BLAST of Bhi10G001172 vs. ExPASy Swiss-Prot
Match: Q9LHA1 (Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 5.7e-64
Identity = 118/202 (58.42%), Postives = 147/202 (72.77%), Query Frame = 0


Query: 1   IIFIAGIDTTAVTLEWALSHLLNNPNVLEKAKVEIDSAIGQERLITEADLPSLNYIQGII 60
           ++ +AG DT+A TLEWA+S+LLN+P VL KAK EID  IG +RL+ E D+  L Y+Q I+
Sbjct: 304 VMILAGTDTSAGTLEWAMSNLLNHPEVLRKAKTEIDDQIGVDRLVEEQDIVKLPYLQHIV 363

Query: 61  SETLRLTPAAPLLVPHCASEDCKIGAYDVPRDTMVLINAWAIHRDPKLWENATKFKPERH 120
           SETLRL P AP+L+PH ASEDC +  YDVPR T++L+NAWAIHRDPKLWE   KFKPER 
Sbjct: 364 SETLRLYPVAPMLLPHLASEDCIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPERF 423

Query: 121 TNPIGTDSYKFLPFGLGRRACPGIGIAQRMATLTLATMIQCFEWEREGSSLVDMSEGE-G 180
               G D  K +PFG+GRR+CPG G+AQR+ TL L +++QCFEWER     +DM E E G
Sbjct: 424 EKK-GEDK-KLMPFGIGRRSCPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRESEKG 483

Query: 181 ITMPKARRLVAKCKPRPIMKAI 202
            TM KA  L A CK RPI+  +
Sbjct: 484 TTMRKATSLQAMCKARPIVHKV 503

[Close]

BLAST of Bhi10G001172 vs. ExPASy Swiss-Prot
Match: Q0WTF4 (Cytochrome P450 81F3 OS=Arabidopsis thaliana OX=3702 GN=CYP81F3 PE=2 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 2.2e-63
Identity = 115/204 (56.37%), Postives = 145/204 (71.08%), Query Frame = 0


Query: 2   IFIAGIDTTAVTLEWALSHLLNNPNVLEKAKVEIDSAIGQERLITEADLPSLNYIQGIIS 61
           + IAG DT+AVTLEWA+S LLN+P  LEKAK+EID  IGQERLI E D+ +L Y+Q I+S
Sbjct: 292 MMIAGTDTSAVTLEWAMSSLLNHPEALEKAKLEIDEKIGQERLIDEPDIANLPYLQNIVS 351

Query: 62  ETLRLTPAAPLLVPHCASEDCKIGAYDVPRDTMVLINAWAIHRDPKLWENATKFKPERHT 121
           ET RL PAAPLLVP   +ED K+G YDVPR TMV++NAWAIHRDP+LW    KFKPER  
Sbjct: 352 ETFRLYPAAPLLVPRSPTEDIKVGGYDVPRGTMVMVNAWAIHRDPELWNEPEKFKPERFN 411

Query: 122 ----NPIGTDSYKFLPFGLGRRACPGIGIAQRMATLTLATMIQCFEWEREGSSLVDMSEG 181
                  G D +K +PFG GRR+CPG G+ Q++ TL L ++IQCF+W++     +DM+E 
Sbjct: 412 GGEGGGRGEDVHKLMPFGNGRRSCPGAGLGQKIVTLALGSLIQCFDWQKVNGEAIDMTET 471

Query: 182 EGITMPKARRLVAKCKPRPIMKAI 202
            G+ M K   L A C+ RPIM  +
Sbjct: 472 PGMAMRKKIPLSALCQSRPIMSKL 495

[Close]

BLAST of Bhi10G001172 vs. ExPASy Swiss-Prot
Match: O65790 (Cytochrome P450 81F1 OS=Arabidopsis thaliana OX=3702 GN=CYP81F1 PE=2 SV=2)

HSP 1 Score: 242.3 bits (617), Expect = 4.8e-63
Identity = 110/202 (54.46%), Postives = 146/202 (72.28%), Query Frame = 0


Query: 2   IFIAGIDTTAVTLEWALSHLLNNPNVLEKAKVEIDSAIGQERLITEADLPSLNYIQGIIS 61
           + +AG +T+AVTLEWA+++LL NP VLEKA+ EID  IG++RLI E+D+  L Y+Q ++S
Sbjct: 297 MMLAGTETSAVTLEWAMANLLRNPEVLEKARSEIDEKIGKDRLIDESDIAVLPYLQNVVS 356

Query: 62  ETLRLTPAAPLLVPHCASEDCKIGAYDVPRDTMVLINAWAIHRDPKLWENATKFKPERHT 121
           ET RL P AP L+P   ++D KIG YDVPRDT+V++NAWAIHRDP++WE   KF P+R+ 
Sbjct: 357 ETFRLFPVAPFLIPRSPTDDMKIGGYDVPRDTIVMVNAWAIHRDPEIWEEPEKFNPDRYN 416

Query: 122 NPIGTD--SYKFLPFGLGRRACPGIGIAQRMATLTLATMIQCFEWEREGSSLVDMSEGEG 181
           +  G+D   YK +PFG GRR CPG G+ QR+ TL L ++IQCFEWE      +DMSE  G
Sbjct: 417 DGCGSDYYVYKLMPFGNGRRTCPGAGLGQRIVTLALGSLIQCFEWENVKGEEMDMSESTG 476

Query: 182 ITMPKARRLVAKCKPRPIMKAI 202
           + M K   L A C+PRPIM  +
Sbjct: 477 LGMRKMDPLRAMCRPRPIMSKL 498

[Close]

BLAST of Bhi10G001172 vs. NCBI nr
Match: XP_038900544.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 416.8 bits (1070), Expect = 1.1e-112
Identity = 203/203 (100.00%), Postives = 203/203 (100.00%), Query Frame = 0


Query: 1   IIFIAGIDTTAVTLEWALSHLLNNPNVLEKAKVEIDSAIGQERLITEADLPSLNYIQGII 60
           IIFIAGIDTTAVTLEWALSHLLNNPNVLEKAKVEIDSAIGQERLITEADLPSLNYIQGII
Sbjct: 291 IIFIAGIDTTAVTLEWALSHLLNNPNVLEKAKVEIDSAIGQERLITEADLPSLNYIQGII 350

Query: 61  SETLRLTPAAPLLVPHCASEDCKIGAYDVPRDTMVLINAWAIHRDPKLWENATKFKPERH 120
           SETLRLTPAAPLLVPHCASEDCKIGAYDVPRDTMVLINAWAIHRDPKLWENATKFKPERH
Sbjct: 351 SETLRLTPAAPLLVPHCASEDCKIGAYDVPRDTMVLINAWAIHRDPKLWENATKFKPERH 410

Query: 121 TNPIGTDSYKFLPFGLGRRACPGIGIAQRMATLTLATMIQCFEWEREGSSLVDMSEGEGI 180
           TNPIGTDSYKFLPFGLGRRACPGIGIAQRMATLTLATMIQCFEWEREGSSLVDMSEGEGI
Sbjct: 411 TNPIGTDSYKFLPFGLGRRACPGIGIAQRMATLTLATMIQCFEWEREGSSLVDMSEGEGI 470

Query: 181 TMPKARRLVAKCKPRPIMKAIFS 204
           TMPKARRLVAKCKPRPIMKAIFS
Sbjct: 471 TMPKARRLVAKCKPRPIMKAIFS 493

[Close]

BLAST of Bhi10G001172 vs. NCBI nr
Match: KAG7011032.1 (Cytochrome P450 81E8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 374.4 bits (960), Expect = 6.2e-100
Identity = 178/203 (87.68%), Postives = 190/203 (93.60%), Query Frame = 0


Query: 1   IIFIAGIDTTAVTLEWALSHLLNNPNVLEKAKVEIDSAIGQERLITEADLPSLNYIQGII 60
           II IAGIDTTAVTLEWALSHLLNNP VLEKAK EIDS+IGQERL+ EADL SLNY+QGII
Sbjct: 292 IILIAGIDTTAVTLEWALSHLLNNPEVLEKAKAEIDSSIGQERLVNEADLSSLNYLQGII 351

Query: 61  SETLRLTPAAPLLVPHCASEDCKIGAYDVPRDTMVLINAWAIHRDPKLWENATKFKPERH 120
           SETLRLTPAAPLLVPHCAS+D KIG YDVPRDT+VLINAWAIHRDP LWE+AT+FKPERH
Sbjct: 352 SETLRLTPAAPLLVPHCASKDTKIGGYDVPRDTIVLINAWAIHRDPSLWEDATRFKPERH 411

Query: 121 TNPIGTDSYKFLPFGLGRRACPGIGIAQRMATLTLATMIQCFEWEREGSSLVDMSEGEGI 180
            NPIG DSYKFLPFGLGRRACPGIGIAQRMATLTL  +IQCFEWEREGSSLVDMSEGEG+
Sbjct: 412 ANPIGIDSYKFLPFGLGRRACPGIGIAQRMATLTLGMLIQCFEWEREGSSLVDMSEGEGV 471

Query: 181 TMPKARRLVAKCKPRPIMKAIFS 204
           TMPKA+RLVAKCKPRPI+K IF+
Sbjct: 472 TMPKAQRLVAKCKPRPIIKTIFT 494

[Close]

BLAST of Bhi10G001172 vs. NCBI nr
Match: XP_022985605.1 (cytochrome P450 81D11-like [Cucurbita maxima])

HSP 1 Score: 373.6 bits (958), Expect = 1.1e-99
Identity = 177/203 (87.19%), Postives = 190/203 (93.60%), Query Frame = 0


Query: 1   IIFIAGIDTTAVTLEWALSHLLNNPNVLEKAKVEIDSAIGQERLITEADLPSLNYIQGII 60
           I  IAGIDTTAVTLEWALSHLLNNP VLEKAKVEIDS+IGQERL+ EADL SLNY+QGII
Sbjct: 292 ITLIAGIDTTAVTLEWALSHLLNNPEVLEKAKVEIDSSIGQERLVNEADLSSLNYLQGII 351

Query: 61  SETLRLTPAAPLLVPHCASEDCKIGAYDVPRDTMVLINAWAIHRDPKLWENATKFKPERH 120
           SETLRLTPAAPLLVPHCAS+D KIG YDVPR+T+VLINAWAIHRDP LWE+AT+FKPERH
Sbjct: 352 SETLRLTPAAPLLVPHCASKDTKIGGYDVPRETIVLINAWAIHRDPSLWEDATRFKPERH 411

Query: 121 TNPIGTDSYKFLPFGLGRRACPGIGIAQRMATLTLATMIQCFEWEREGSSLVDMSEGEGI 180
            NPIG DSYKFLPFGLGRRACPG+GIAQRMATLTL  +IQCFEWEREGSSLVDMSEGEG+
Sbjct: 412 ANPIGIDSYKFLPFGLGRRACPGVGIAQRMATLTLGMLIQCFEWEREGSSLVDMSEGEGV 471

Query: 181 TMPKARRLVAKCKPRPIMKAIFS 204
           TMPKA+RLVAKCKPRPIMK IF+
Sbjct: 472 TMPKAQRLVAKCKPRPIMKTIFN 494

[Close]

BLAST of Bhi10G001172 vs. NCBI nr
Match: KGN63298.1 (hypothetical protein Csa_021868 [Cucumis sativus])

HSP 1 Score: 370.5 bits (950), Expect = 9.0e-99
Identity = 172/203 (84.73%), Postives = 190/203 (93.60%), Query Frame = 0


Query: 1   IIFIAGIDTTAVTLEWALSHLLNNPNVLEKAKVEIDSAIGQERLITEADLPSLNYIQGII 60
           II IAGIDT AVTLEWALSHLLNNP+VLEKAK+EID+ +GQ+RL+ EADLPSL Y+QGII
Sbjct: 293 IILIAGIDTAAVTLEWALSHLLNNPDVLEKAKIEIDNVVGQKRLVNEADLPSLTYLQGII 352

Query: 61  SETLRLTPAAPLLVPHCASEDCKIGAYDVPRDTMVLINAWAIHRDPKLWENATKFKPERH 120
            ETLRL+PAAPLLVPHC+SEDCKIG YDVPRDT+V+INAWAIHRDP LWE+AT FKPERH
Sbjct: 353 FETLRLSPAAPLLVPHCSSEDCKIGGYDVPRDTIVIINAWAIHRDPNLWEDATSFKPERH 412

Query: 121 TNPIGTDSYKFLPFGLGRRACPGIGIAQRMATLTLATMIQCFEWEREGSSLVDMSEGEGI 180
           TNPIG +SYKFLPFGLGRRACPGIGIAQRM  LTLATMIQCFEW+REGSSLVDMSEGEG+
Sbjct: 413 TNPIGVESYKFLPFGLGRRACPGIGIAQRMTNLTLATMIQCFEWKREGSSLVDMSEGEGL 472

Query: 181 TMPKARRLVAKCKPRPIMKAIFS 204
           TMPKA+ L+AKCKPRPIMKA+FS
Sbjct: 473 TMPKAQPLIAKCKPRPIMKAMFS 495

[Close]

BLAST of Bhi10G001172 vs. NCBI nr
Match: XP_011650031.2 (cytochrome P450 81Q32 [Cucumis sativus])

HSP 1 Score: 370.5 bits (950), Expect = 9.0e-99
Identity = 172/203 (84.73%), Postives = 190/203 (93.60%), Query Frame = 0


Query: 1   IIFIAGIDTTAVTLEWALSHLLNNPNVLEKAKVEIDSAIGQERLITEADLPSLNYIQGII 60
           II IAGIDT AVTLEWALSHLLNNP+VLEKAK+EID+ +GQ+RL+ EADLPSL Y+QGII
Sbjct: 338 IILIAGIDTAAVTLEWALSHLLNNPDVLEKAKIEIDNVVGQKRLVNEADLPSLTYLQGII 397

Query: 61  SETLRLTPAAPLLVPHCASEDCKIGAYDVPRDTMVLINAWAIHRDPKLWENATKFKPERH 120
            ETLRL+PAAPLLVPHC+SEDCKIG YDVPRDT+V+INAWAIHRDP LWE+AT FKPERH
Sbjct: 398 FETLRLSPAAPLLVPHCSSEDCKIGGYDVPRDTIVIINAWAIHRDPNLWEDATSFKPERH 457

Query: 121 TNPIGTDSYKFLPFGLGRRACPGIGIAQRMATLTLATMIQCFEWEREGSSLVDMSEGEGI 180
           TNPIG +SYKFLPFGLGRRACPGIGIAQRM  LTLATMIQCFEW+REGSSLVDMSEGEG+
Sbjct: 458 TNPIGVESYKFLPFGLGRRACPGIGIAQRMTNLTLATMIQCFEWKREGSSLVDMSEGEGL 517

Query: 181 TMPKARRLVAKCKPRPIMKAIFS 204
           TMPKA+ L+AKCKPRPIMKA+FS
Sbjct: 518 TMPKAQPLIAKCKPRPIMKAMFS 540

[Close]

BLAST of Bhi10G001172 vs. ExPASy TrEMBL
Match: A0A6J1JBS5 (cytochrome P450 81D11-like OS=Cucurbita maxima OX=3661 GN=LOC111483618 PE=3 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 5.1e-100
Identity = 177/203 (87.19%), Postives = 190/203 (93.60%), Query Frame = 0


Query: 1   IIFIAGIDTTAVTLEWALSHLLNNPNVLEKAKVEIDSAIGQERLITEADLPSLNYIQGII 60
           I  IAGIDTTAVTLEWALSHLLNNP VLEKAKVEIDS+IGQERL+ EADL SLNY+QGII
Sbjct: 292 ITLIAGIDTTAVTLEWALSHLLNNPEVLEKAKVEIDSSIGQERLVNEADLSSLNYLQGII 351

Query: 61  SETLRLTPAAPLLVPHCASEDCKIGAYDVPRDTMVLINAWAIHRDPKLWENATKFKPERH 120
           SETLRLTPAAPLLVPHCAS+D KIG YDVPR+T+VLINAWAIHRDP LWE+AT+FKPERH
Sbjct: 352 SETLRLTPAAPLLVPHCASKDTKIGGYDVPRETIVLINAWAIHRDPSLWEDATRFKPERH 411

Query: 121 TNPIGTDSYKFLPFGLGRRACPGIGIAQRMATLTLATMIQCFEWEREGSSLVDMSEGEGI 180
            NPIG DSYKFLPFGLGRRACPG+GIAQRMATLTL  +IQCFEWEREGSSLVDMSEGEG+
Sbjct: 412 ANPIGIDSYKFLPFGLGRRACPGVGIAQRMATLTLGMLIQCFEWEREGSSLVDMSEGEGV 471

Query: 181 TMPKARRLVAKCKPRPIMKAIFS 204
           TMPKA+RLVAKCKPRPIMK IF+
Sbjct: 472 TMPKAQRLVAKCKPRPIMKTIFN 494

[Close]

BLAST of Bhi10G001172 vs. ExPASy TrEMBL
Match: A0A0A0LNA4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G425750 PE=3 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 4.3e-99
Identity = 172/203 (84.73%), Postives = 190/203 (93.60%), Query Frame = 0


Query: 1   IIFIAGIDTTAVTLEWALSHLLNNPNVLEKAKVEIDSAIGQERLITEADLPSLNYIQGII 60
           II IAGIDT AVTLEWALSHLLNNP+VLEKAK+EID+ +GQ+RL+ EADLPSL Y+QGII
Sbjct: 293 IILIAGIDTAAVTLEWALSHLLNNPDVLEKAKIEIDNVVGQKRLVNEADLPSLTYLQGII 352

Query: 61  SETLRLTPAAPLLVPHCASEDCKIGAYDVPRDTMVLINAWAIHRDPKLWENATKFKPERH 120
            ETLRL+PAAPLLVPHC+SEDCKIG YDVPRDT+V+INAWAIHRDP LWE+AT FKPERH
Sbjct: 353 FETLRLSPAAPLLVPHCSSEDCKIGGYDVPRDTIVIINAWAIHRDPNLWEDATSFKPERH 412

Query: 121 TNPIGTDSYKFLPFGLGRRACPGIGIAQRMATLTLATMIQCFEWEREGSSLVDMSEGEGI 180
           TNPIG +SYKFLPFGLGRRACPGIGIAQRM  LTLATMIQCFEW+REGSSLVDMSEGEG+
Sbjct: 413 TNPIGVESYKFLPFGLGRRACPGIGIAQRMTNLTLATMIQCFEWKREGSSLVDMSEGEGL 472

Query: 181 TMPKARRLVAKCKPRPIMKAIFS 204
           TMPKA+ L+AKCKPRPIMKA+FS
Sbjct: 473 TMPKAQPLIAKCKPRPIMKAMFS 495

[Close]

BLAST of Bhi10G001172 vs. ExPASy TrEMBL
Match: A0A5A7SSH4 (Cytochrome P450 81D1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold232G00910 PE=3 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 3.7e-98
Identity = 168/203 (82.76%), Postives = 191/203 (94.09%), Query Frame = 0


Query: 1   IIFIAGIDTTAVTLEWALSHLLNNPNVLEKAKVEIDSAIGQERLITEADLPSLNYIQGII 60
           II IAG+DTTAVTLEWAL+HLLNNP+VL+KAK+EID+ +GQERL++E DLPSL Y+QGII
Sbjct: 293 IILIAGVDTTAVTLEWALAHLLNNPDVLQKAKLEIDNVVGQERLVSEVDLPSLTYLQGII 352

Query: 61  SETLRLTPAAPLLVPHCASEDCKIGAYDVPRDTMVLINAWAIHRDPKLWENATKFKPERH 120
            ETLRL+PAAPLLVPHC+ EDCKIG YDVPRDT+V+INAWAIHRDP LWE+A+ FKPERH
Sbjct: 353 FETLRLSPAAPLLVPHCSFEDCKIGGYDVPRDTIVIINAWAIHRDPNLWEDASSFKPERH 412

Query: 121 TNPIGTDSYKFLPFGLGRRACPGIGIAQRMATLTLATMIQCFEWEREGSSLVDMSEGEGI 180
           TNPIG +SYKF+PFGLGRRACPGIGIAQ+MA LTLATMIQCFEWEREGSSLVDMSEGEG+
Sbjct: 413 TNPIGVESYKFIPFGLGRRACPGIGIAQKMANLTLATMIQCFEWEREGSSLVDMSEGEGL 472

Query: 181 TMPKARRLVAKCKPRPIMKAIFS 204
           TMPKA+ L+AKCKPRPIMKA+FS
Sbjct: 473 TMPKAQPLIAKCKPRPIMKAVFS 495

[Close]

BLAST of Bhi10G001172 vs. ExPASy TrEMBL
Match: A0A1S3C312 (cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103495915 PE=3 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 3.7e-98
Identity = 168/203 (82.76%), Postives = 191/203 (94.09%), Query Frame = 0


Query: 1   IIFIAGIDTTAVTLEWALSHLLNNPNVLEKAKVEIDSAIGQERLITEADLPSLNYIQGII 60
           II IAG+DTTAVTLEWAL+HLLNNP+VL+KAK+EID+ +GQERL++E DLPSL Y+QGII
Sbjct: 180 IILIAGVDTTAVTLEWALAHLLNNPDVLQKAKLEIDNVVGQERLVSEVDLPSLTYLQGII 239

Query: 61  SETLRLTPAAPLLVPHCASEDCKIGAYDVPRDTMVLINAWAIHRDPKLWENATKFKPERH 120
            ETLRL+PAAPLLVPHC+ EDCKIG YDVPRDT+V+INAWAIHRDP LWE+A+ FKPERH
Sbjct: 240 FETLRLSPAAPLLVPHCSFEDCKIGGYDVPRDTIVIINAWAIHRDPNLWEDASSFKPERH 299

Query: 121 TNPIGTDSYKFLPFGLGRRACPGIGIAQRMATLTLATMIQCFEWEREGSSLVDMSEGEGI 180
           TNPIG +SYKF+PFGLGRRACPGIGIAQ+MA LTLATMIQCFEWEREGSSLVDMSEGEG+
Sbjct: 300 TNPIGVESYKFIPFGLGRRACPGIGIAQKMANLTLATMIQCFEWEREGSSLVDMSEGEGL 359

Query: 181 TMPKARRLVAKCKPRPIMKAIFS 204
           TMPKA+ L+AKCKPRPIMKA+FS
Sbjct: 360 TMPKAQPLIAKCKPRPIMKAVFS 382

[Close]

BLAST of Bhi10G001172 vs. ExPASy TrEMBL
Match: A0A6J1D8L6 (cytochrome P450 81E8-like OS=Momordica charantia OX=3673 GN=LOC111017928 PE=3 SV=1)

HSP 1 Score: 335.1 bits (858), Expect = 2.0e-88
Identity = 155/203 (76.35%), Postives = 179/203 (88.18%), Query Frame = 0


Query: 1   IIFIAGIDTTAVTLEWALSHLLNNPNVLEKAKVEIDSAIGQERLITEADLPSLNYIQGII 60
           +I IAGIDT  VTLEWALSHLLNNP VLEKAK EID++IGQER++ EADL SL+Y+QGII
Sbjct: 292 VILIAGIDTIGVTLEWALSHLLNNPEVLEKAKAEIDNSIGQERMVNEADLSSLSYVQGII 351

Query: 61  SETLRLTPAAPLLVPHCASEDCKIGAYDVPRDTMVLINAWAIHRDPKLWENATKFKPERH 120
            ETLRLTPAAPLLV HC SEDCKIG YD+PRD +VLINAWAIHRDP+LWE+AT F+PERH
Sbjct: 352 FETLRLTPAAPLLVAHCPSEDCKIGGYDIPRDAIVLINAWAIHRDPELWEDATSFRPERH 411

Query: 121 TNPIGTDSYKFLPFGLGRRACPGIGIAQRMATLTLATMIQCFEWEREGSSLVDMSEGEGI 180
           TN IG DSYK LPFGLGRRACPG+G+AQR+  LTLA++IQCFEW+R  S LVDM+EG G+
Sbjct: 412 TNAIGVDSYKLLPFGLGRRACPGMGMAQRVVGLTLASLIQCFEWKRASSLLVDMTEGGGL 471

Query: 181 TMPKARRLVAKCKPRPIMKAIFS 204
           TMPKA+ LVAKC+PRPIM+AI +
Sbjct: 472 TMPKAQPLVAKCRPRPIMRAILN 494

The following BLAST results are available for this feature:

BLAST of Bhi10G001172 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G37360.1	1.4e-70	62.00	cytochrome P450, family 81, subfamily D, polypeptide 2	[more]
AT1G66540.1	3.9e-68	59.00	Cytochrome P450 superfamily protein	[more]
AT1G66540.2	3.9e-68	59.00	Cytochrome P450 superfamily protein	[more]
AT4G37340.1	8.7e-68	59.61	cytochrome P450, family 81, subfamily D, polypeptide 3	[more]
AT4G37370.1	5.7e-67	57.92	cytochrome P450, family 81, subfamily D, polypeptide 8	[more]

BLAST of Bhi10G001172 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
W8JMU7	5.0e-68	57.14	Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1	[more]
Q9FG65	1.8e-65	60.10	Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1	[more]
Q9LHA1	5.7e-64	58.42	Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1	[more]
Q0WTF4	2.2e-63	56.37	Cytochrome P450 81F3 OS=Arabidopsis thaliana OX=3702 GN=CYP81F3 PE=2 SV=1	[more]
O65790	4.8e-63	54.46	Cytochrome P450 81F1 OS=Arabidopsis thaliana OX=3702 GN=CYP81F1 PE=2 SV=2	[more]

BLAST of Bhi10G001172 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038900544.1	1.1e-112	100.00	cytochrome P450 81Q32-like [Benincasa hispida]	[more]
KAG7011032.1	6.2e-100	87.68	Cytochrome P450 81E8, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022985605.1	1.1e-99	87.19	cytochrome P450 81D11-like [Cucurbita maxima]	[more]
KGN63298.1	9.0e-99	84.73	hypothetical protein Csa_021868 [Cucumis sativus]	[more]
XP_011650031.2	9.0e-99	84.73	cytochrome P450 81Q32 [Cucumis sativus]	[more]

BLAST of Bhi10G001172 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JBS5	5.1e-100	87.19	cytochrome P450 81D11-like OS=Cucurbita maxima OX=3661 GN=LOC111483618 PE=3 SV=1	[more]
A0A0A0LNA4	4.3e-99	84.73	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G425750 PE=3 SV=1	[more]
A0A5A7SSH4	3.7e-98	82.76	Cytochrome P450 81D1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S3C312	3.7e-98	82.76	cytochrome P450 81D1-like OS=Cucumis melo OX=3656 GN=LOC103495915 PE=3 SV=1	[more]
A0A6J1D8L6	2.0e-88	76.35	cytochrome P450 81E8-like OS=Momordica charantia OX=3673 GN=LOC111017928 PE=3 SV...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 57..75 score: 35.65 coord: 14..40 score: 38.12 coord: 98..122 score: 33.04 coord: 131..141 score: 59.43 coord: 141..164 score: 31.49
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 5..22 score: 43.32 coord: 132..141 score: 60.23 coord: 58..69 score: 39.18 coord: 141..152 score: 29.95
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 2..186 e-value: 1.0E-62 score: 212.4
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..197 e-value: 3.0E-69 score: 235.8
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 2..195
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 2..200
None	No IPR available	PANTHER	PTHR24298:SF836	CYTOCHROME P450, FAMILY 81, SUBFAMILY D, POLYPEPTIDE 2-RELATED	coord: 2..200
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 134..143

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi10M001172	Bhi10M001172	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016709	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen