Bhi10G001167 (gene) Wax gourd (B227) v1

Overview


Name	Bhi10G001167
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	cytochrome P450 81D1-like
Location	chr10: 35903057 .. 35903442 (-)
RNA-Seq Expression	Bhi10G001167
Synteny	Bhi10G001167

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AGTGTAAAATAGAAGGATATGATGTTCCATGTAATACAATTATATTGATCAATGCTTGGGCTGTTCATAGAGATTCAAGTATTTGGGAAGATGCCGCAAGCTTTAAACCTGAAAGACATGCAAGTGGGATTGAAGTTGATTATTCATATAAACTAATACCATTTGGAAGTGGGAGGAGAGCATGTCCTGGTGTAGGAATGGCTCAACGAGTGGCTGGTTTGACTCTAGCATCATCGATACAAAGCTTTGAATGGGAAAGAGTGAACAACTCATTAATTGACATGAGTGAAGGTAAAGGACTCACAATGCCTAAAGCTCAACCATTGGTAGCTAAGTGTAAACCACGTCTAATCATGAAAGTCATTTTGAACGCACGTAGCAATTGA

mRNA sequence

AGTGTAAAATAGAAGGATATGATGTTCCATGTAATACAATTATATTGATCAATGCTTGGGCTGTTCATAGAGATTCAAGTATTTGGGAAGATGCCGCAAGCTTTAAACCTGAAAGACATGCAAGTGGGATTGAAGTTGATTATTCATATAAACTAATACCATTTGGAAGTGGGAGGAGAGCATGTCCTGGTGTAGGAATGGCTCAACGAGTGGCTGGTTTGACTCTAGCATCATCGATACAAAGCTTTGAATGGGAAAGAGTGAACAACTCATTAATTGACATGAGTGAAGGTAAAGGACTCACAATGCCTAAAGCTCAACCATTGGTAGCTAAGTGTAAACCACGTCTAATCATGAAAGTCATTTTGAACGCACGTAGCAATTGA

Coding sequence (CDS)

AGTGTAAAATAGAAGGATATGATGTTCCATGTAATACAATTATATTGATCAATGCTTGGGCTGTTCATAGAGATTCAAGTATTTGGGAAGATGCCGCAAGCTTTAAACCTGAAAGACATGCAAGTGGGATTGAAGTTGATTATTCATATAAACTAATACCATTTGGAAGTGGGAGGAGAGCATGTCCTGGTGTAGGAATGGCTCAACGAGTGGCTGGTTTGACTCTAGCATCATCGATACAAAGCTTTGAATGGGAAAGAGTGAACAACTCATTAATTGACATGAGTGAAGGTAAAGGACTCACAATGCCTAAAGCTCAACCATTGGTAGCTAAGTGTAAACCACGTCTAATCATGAAAGTCATTTTGAACGCACGTAGCAATTGA

Protein sequence

CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVDYSYKLIPFGSGRRACPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIMKVILNARSN

Homology

[Close]

BLAST of Bhi10G001167 vs. TAIR 10
Match: AT1G66540.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 148.7 bits (374), Expect = 3.2e-36
Identity = 65/115 (56.52%), Postives = 85/115 (73.91%), Query Frame = 0


Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVDYSYKLIPFGSGRRA 60
           CK+ GYD+PC T++L+N WA+HRD  +W+D ASFKPER     E   ++KL+ FG GRRA
Sbjct: 264 CKVGGYDMPCGTMLLVNVWAIHRDPRLWDDPASFKPERFEKEGE---THKLLTFGLGRRA 323

Query: 61  CPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPR 116
           CPG G+A+R+  L+L S IQ FEWER+    +DM+EG GLTMP+A PLVA C+ R
Sbjct: 324 CPGSGLARRLVSLSLGSLIQCFEWERIGEEEVDMTEGGGLTMPRAIPLVAMCRAR 375

[Close]

BLAST of Bhi10G001167 vs. TAIR 10
Match: AT1G66540.2 (Cytochrome P450 superfamily protein )

HSP 1 Score: 148.7 bits (374), Expect = 3.2e-36
Identity = 65/115 (56.52%), Postives = 85/115 (73.91%), Query Frame = 0


Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVDYSYKLIPFGSGRRA 60
           CK+ GYD+PC T++L+N WA+HRD  +W+D ASFKPER     E   ++KL+ FG GRRA
Sbjct: 205 CKVGGYDMPCGTMLLVNVWAIHRDPRLWDDPASFKPERFEKEGE---THKLLTFGLGRRA 264

Query: 61  CPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPR 116
           CPG G+A+R+  L+L S IQ FEWER+    +DM+EG GLTMP+A PLVA C+ R
Sbjct: 265 CPGSGLARRLVSLSLGSLIQCFEWERIGEEEVDMTEGGGLTMPRAIPLVAMCRAR 316

[Close]

BLAST of Bhi10G001167 vs. TAIR 10
Match: AT4G37430.1 (cytochrome P450, family 91, subfamily A, polypeptide 2 )

HSP 1 Score: 144.8 bits (364), Expect = 4.7e-35
Identity = 67/122 (54.92%), Postives = 82/122 (67.21%), Query Frame = 0


Query: 2   KIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVD-YSYKLIPFGSGRRA 61
           KI GYDVP +TI+++NAWA+HRD  IWE+   F P+R+  G   D Y YKL+PFG+GRR 
Sbjct: 378 KIGGYDVPRDTIVMVNAWAIHRDPEIWEEPEKFNPDRYNDGCGSDYYVYKLMPFGNGRRT 437

Query: 62  CPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIMKV 121
           CPG G+ QR+  L L S IQ FEWE V    +DMSE  GL M K  PL A C+PR IM  
Sbjct: 438 CPGAGLGQRIVTLALGSLIQCFEWENVKGEEMDMSESTGLGMRKMDPLRAMCRPRPIMSK 497

Query: 122 IL 123
           +L
Sbjct: 498 LL 499

[Close]

BLAST of Bhi10G001167 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 144.4 bits (363), Expect = 6.1e-35
Identity = 68/122 (55.74%), Postives = 86/122 (70.49%), Query Frame = 0


Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVDYSYKLIPFGSGRRA 60
           CK+ GYD+P  T++L NAWA+HRD  +W+D  SFKPER     E   + KL+PFG GRRA
Sbjct: 377 CKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERFEKEGE---AKKLMPFGLGRRA 436

Query: 61  CPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIMKV 120
           CPG G+AQR+  L+L S IQ FEWER+    +DM+EG GLTMPKA+PL A C+ R  +  
Sbjct: 437 CPGSGLAQRLVTLSLGSLIQCFEWERIGEEEVDMTEGPGLTMPKARPLEAMCRARDFVGK 495

Query: 121 IL 123
           IL
Sbjct: 497 IL 495

[Close]

BLAST of Bhi10G001167 vs. TAIR 10
Match: AT5G67310.1 (cytochrome P450, family 81, subfamily G, polypeptide 1 )

HSP 1 Score: 141.7 bits (356), Expect = 4.0e-34
Identity = 67/123 (54.47%), Postives = 88/123 (71.54%), Query Frame = 0


Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVDYSYKLIPFGSGRRA 60
           C++ G+D+P  T + INAWA+ RD ++W+D  +FKPER  S     +  K +PFG GRRA
Sbjct: 388 CEVAGFDIPRRTWLFINAWAIQRDPNVWDDPETFKPERFES---ETHRGKFLPFGIGRRA 447

Query: 61  CPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIM-K 120
           CPG+G+AQ V  L L S IQ F+WER N+  +DMSEGKGLTMPK+ PLVAKCK   I+ K
Sbjct: 448 CPGMGLAQLVLSLALGSLIQCFDWERDNDVAVDMSEGKGLTMPKSVPLVAKCKSLPILDK 507

Query: 121 VIL 123
           ++L
Sbjct: 508 LVL 507

[Close]

BLAST of Bhi10G001167 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 3.6e-40
Identity = 75/126 (59.52%), Postives = 97/126 (76.98%), Query Frame = 0


Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVDYSYKLIPFGSGRRA 60
           CK++GYDVP  TI+L+NAWA+HRD   W++   FKPERH  G+E++ S KL+PFG GRR+
Sbjct: 376 CKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPERH-GGVELEPS-KLMPFGMGRRS 435

Query: 61  CPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIMKV 120
           CPG G+AQRV GLTL + IQ FEW+R+  + IDM+EG GLTMPKAQPL A CKPR I+  
Sbjct: 436 CPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSGLTMPKAQPLEALCKPRNILHK 495

Query: 121 ILNARS 127
           +++  S
Sbjct: 496 VVSETS 499

[Close]

BLAST of Bhi10G001167 vs. ExPASy Swiss-Prot
Match: O65790 (Cytochrome P450 81F1 OS=Arabidopsis thaliana OX=3702 GN=CYP81F1 PE=2 SV=2)

HSP 1 Score: 144.8 bits (364), Expect = 6.6e-34
Identity = 67/122 (54.92%), Postives = 82/122 (67.21%), Query Frame = 0


Query: 2   KIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVD-YSYKLIPFGSGRRA 61
           KI GYDVP +TI+++NAWA+HRD  IWE+   F P+R+  G   D Y YKL+PFG+GRR 
Sbjct: 378 KIGGYDVPRDTIVMVNAWAIHRDPEIWEEPEKFNPDRYNDGCGSDYYVYKLMPFGNGRRT 437

Query: 62  CPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIMKV 121
           CPG G+ QR+  L L S IQ FEWE V    +DMSE  GL M K  PL A C+PR IM  
Sbjct: 438 CPGAGLGQRIVTLALGSLIQCFEWENVKGEEMDMSESTGLGMRKMDPLRAMCRPRPIMSK 497

Query: 122 IL 123
           +L
Sbjct: 498 LL 499

[Close]

BLAST of Bhi10G001167 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 140.6 bits (353), Expect = 1.2e-32
Identity = 68/123 (55.28%), Postives = 83/123 (67.48%), Query Frame = 0


Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVDYSYKLIPFGSGRRA 60
           CKI  YD+P  T +L+NAWA+HRD + W+D  SFKPER     E   + KL+ FG GRRA
Sbjct: 383 CKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEKEEE---AQKLLAFGLGRRA 442

Query: 61  CPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIMKV 120
           CPG G+AQR+ GL L S IQ FEWERV N  +DM EG G T+PKA PL A CK R  +  
Sbjct: 443 CPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNTVPKAIPLKAICKARPFLHK 502

Query: 121 ILN 124
           I++
Sbjct: 503 IIS 502

[Close]

BLAST of Bhi10G001167 vs. ExPASy Swiss-Prot
Match: Q9LHA1 (Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 6.8e-31
Identity = 67/125 (53.60%), Postives = 82/125 (65.60%), Query Frame = 0


Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVDYSYKLIPFGSGRRA 60
           C ++GYDVP  TIIL+NAWA+HRD  +WE+   FKPER     E     KL+PFG GRR+
Sbjct: 385 CIVDGYDVPRGTIILVNAWAIHRDPKLWEEPEKFKPERFEKKGE---DKKLMPFGIGRRS 444

Query: 61  CPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEG-KGLTMPKAQPLVAKCKPRLIMK 120
           CPG G+AQR+  L L S +Q FEWERV    +DM E  KG TM KA  L A CK R I+ 
Sbjct: 445 CPGSGLAQRLVTLALGSLVQCFEWERVEEKYLDMRESEKGTTMRKATSLQAMCKARPIVH 504

Query: 121 VILNA 125
            +L+A
Sbjct: 505 KVLDA 506

[Close]

BLAST of Bhi10G001167 vs. ExPASy Swiss-Prot
Match: Q6WNR0 (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 2.2e-29
Identity = 61/121 (50.41%), Postives = 80/121 (66.12%), Query Frame = 0


Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVDYSYKLIPFGSGRRA 60
           C + GY VP +TI+LINAWA+HRD   W +A +FKPER     E++   K+I FG GRRA
Sbjct: 379 CIMGGYKVPRDTIVLINAWAIHRDPETWSEATTFKPERFDKKGELE---KMIAFGMGRRA 438

Query: 61  CPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIMKV 120
           CPG G+A R   +TLA  +Q F+W+R+N+  IDMSE  G TM K  PL A CK R ++  
Sbjct: 439 CPGEGLALRAISMTLALLVQCFDWKRINDEKIDMSERDGFTMTKLLPLKAMCKTRPVVNK 496

Query: 121 I 122
           +
Sbjct: 499 V 496

[Close]

BLAST of Bhi10G001167 vs. NCBI nr
Match: XP_038901939.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 243.4 bits (620), Expect = 1.0e-60
Identity = 115/127 (90.55%), Postives = 120/127 (94.49%), Query Frame = 0


Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVDYSYKLIPFGSGRRA 60
           CKIEGYDVP NTIILIN WA+HRDSSIWEDA SFKPERHASGIEVDYSYKLIPFGSGRRA
Sbjct: 189 CKIEGYDVPRNTIILINVWAIHRDSSIWEDATSFKPERHASGIEVDYSYKLIPFGSGRRA 248

Query: 61  CPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIMKV 120
           CPGVGMAQRV GLTLAS IQSFEW+RVNNSLIDM+EGKGLTMPK +PLVAKCKPR IMK+
Sbjct: 249 CPGVGMAQRVVGLTLASLIQSFEWKRVNNSLIDMNEGKGLTMPKTRPLVAKCKPRPIMKI 308

Query: 121 ILNARSN 128
           ILNARSN
Sbjct: 309 ILNARSN 315

[Close]

BLAST of Bhi10G001167 vs. NCBI nr
Match: XP_023512186.1 (isoflavone 2'-hydroxylase-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023512187.1 isoflavone 2'-hydroxylase-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 209.5 bits (532), Expect = 1.7e-50
Identity = 99/122 (81.15%), Postives = 111/122 (90.98%), Query Frame = 0


Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVDY-SYKLIPFGSGRR 60
           CKIEGYDVP +TIILINAWA+HRD ++WEDA SFKPERHA+G++    SYKLIPFG GRR
Sbjct: 370 CKIEGYDVPRDTIILINAWAMHRDPNLWEDATSFKPERHANGMKNGADSYKLIPFGLGRR 429

Query: 61  ACPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIMK 120
           ACPGVGMAQRVAGLTLAS IQ FEWER+NNSL+DMSEGKG+TMPKAQPLVAKCKPR IMK
Sbjct: 430 ACPGVGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLVAKCKPRPIMK 489

Query: 121 VI 122
           ++
Sbjct: 490 LV 491

[Close]

BLAST of Bhi10G001167 vs. NCBI nr
Match: XP_022985609.1 (isoflavone 2'-hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 208.4 bits (529), Expect = 3.7e-50
Identity = 98/122 (80.33%), Postives = 111/122 (90.98%), Query Frame = 0


Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVD-YSYKLIPFGSGRR 60
           CKIEGYDVP +TIILINAWA+HRD ++WEDA SFKPERHA+G++    SYKLIPFG GRR
Sbjct: 371 CKIEGYDVPRDTIILINAWAMHRDPNLWEDATSFKPERHANGMKNGAESYKLIPFGLGRR 430

Query: 61  ACPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIMK 120
           ACPG+GMAQRVAGLTLAS IQ FEWER+NNSL+DMSEGKG+TMPKAQPLVAKCKPR IMK
Sbjct: 431 ACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLVAKCKPRPIMK 490

Query: 121 VI 122
           ++
Sbjct: 491 LV 492

[Close]

BLAST of Bhi10G001167 vs. NCBI nr
Match: XP_022943757.1 (isoflavone 2'-hydroxylase-like [Cucurbita moschata])

HSP 1 Score: 206.8 bits (525), Expect = 1.1e-49
Identity = 97/122 (79.51%), Postives = 110/122 (90.16%), Query Frame = 0


Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVD-YSYKLIPFGSGRR 60
           CKIEGYDVP +TII INAWA+HRD ++WEDA SFKPERHA+G++    SYKLIPFG GRR
Sbjct: 370 CKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESYKLIPFGLGRR 429

Query: 61  ACPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIMK 120
           ACPG+GMAQRVAGLTLAS IQ FEWER+NNSL+DMSEGKG+TMPKAQPLVAKCKPR IMK
Sbjct: 430 ACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLVAKCKPRPIMK 489

Query: 121 VI 122
           ++
Sbjct: 490 LV 491

[Close]

BLAST of Bhi10G001167 vs. NCBI nr
Match: XP_022943410.1 (isoflavone 2'-hydroxylase-like [Cucurbita moschata] >KAG7011036.1 Isoflavone 2'-hydroxylase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 206.8 bits (525), Expect = 1.1e-49
Identity = 97/122 (79.51%), Postives = 110/122 (90.16%), Query Frame = 0


Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVD-YSYKLIPFGSGRR 60
           CKIEGYDVP +TII INAWA+HRD ++WEDA SFKPERHA+G++    SYKLIPFG GRR
Sbjct: 371 CKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESYKLIPFGLGRR 430

Query: 61  ACPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIMK 120
           ACPG+GMAQRVAGLTLAS IQ FEWER+NNSL+DMSEGKG+TMPKAQPLVAKCKPR IMK
Sbjct: 431 ACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLVAKCKPRPIMK 490

Query: 121 VI 122
           ++
Sbjct: 491 LV 492

[Close]

BLAST of Bhi10G001167 vs. ExPASy TrEMBL
Match: A0A6J1JDS1 (isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111483622 PE=3 SV=1)

HSP 1 Score: 208.4 bits (529), Expect = 1.8e-50
Identity = 98/122 (80.33%), Postives = 111/122 (90.98%), Query Frame = 0


Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVD-YSYKLIPFGSGRR 60
           CKIEGYDVP +TIILINAWA+HRD ++WEDA SFKPERHA+G++    SYKLIPFG GRR
Sbjct: 371 CKIEGYDVPRDTIILINAWAMHRDPNLWEDATSFKPERHANGMKNGAESYKLIPFGLGRR 430

Query: 61  ACPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIMK 120
           ACPG+GMAQRVAGLTLAS IQ FEWER+NNSL+DMSEGKG+TMPKAQPLVAKCKPR IMK
Sbjct: 431 ACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLVAKCKPRPIMK 490

Query: 121 VI 122
           ++
Sbjct: 491 LV 492

[Close]

BLAST of Bhi10G001167 vs. ExPASy TrEMBL
Match: A0A6J1FXS5 (isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111448183 PE=3 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 5.2e-50
Identity = 97/122 (79.51%), Postives = 110/122 (90.16%), Query Frame = 0


Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVD-YSYKLIPFGSGRR 60
           CKIEGYDVP +TII INAWA+HRD ++WEDA SFKPERHA+G++    SYKLIPFG GRR
Sbjct: 371 CKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESYKLIPFGLGRR 430

Query: 61  ACPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIMK 120
           ACPG+GMAQRVAGLTLAS IQ FEWER+NNSL+DMSEGKG+TMPKAQPLVAKCKPR IMK
Sbjct: 431 ACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLVAKCKPRPIMK 490

Query: 121 VI 122
           ++
Sbjct: 491 LV 492

[Close]

BLAST of Bhi10G001167 vs. ExPASy TrEMBL
Match: A0A6J1FTX9 (isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111448413 PE=3 SV=1)

HSP 1 Score: 206.8 bits (525), Expect = 5.2e-50
Identity = 97/122 (79.51%), Postives = 110/122 (90.16%), Query Frame = 0


Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVD-YSYKLIPFGSGRR 60
           CKIEGYDVP +TII INAWA+HRD ++WEDA SFKPERHA+G++    SYKLIPFG GRR
Sbjct: 370 CKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESYKLIPFGLGRR 429

Query: 61  ACPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIMK 120
           ACPG+GMAQRVAGLTLAS IQ FEWER+NNSL+DMSEGKG+TMPKAQPLVAKCKPR IMK
Sbjct: 430 ACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLVAKCKPRPIMK 489

Query: 121 VI 122
           ++
Sbjct: 490 LV 491

[Close]

BLAST of Bhi10G001167 vs. ExPASy TrEMBL
Match: A0A6J1D5S6 (isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017884 PE=3 SV=1)

HSP 1 Score: 205.7 bits (522), Expect = 1.2e-49
Identity = 96/122 (78.69%), Postives = 109/122 (89.34%), Query Frame = 0


Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVDYSYKLIPFGSGRRA 60
           CKIEGYD+P +TIILINAWA+HRDS+IWEDA SFKPERHA+ +    SYKL+PFG GRRA
Sbjct: 456 CKIEGYDIPRDTIILINAWAIHRDSNIWEDATSFKPERHANTMGGVDSYKLLPFGLGRRA 515

Query: 61  CPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIMKV 120
           CPG+GMAQRV GLTLAS IQ FEWERVN+SL+DM+EG+GLTMPKAQPLVAKCKPR I+K 
Sbjct: 516 CPGMGMAQRVVGLTLASLIQCFEWERVNSSLVDMTEGEGLTMPKAQPLVAKCKPRPILKA 575

Query: 121 IL 123
           IL
Sbjct: 576 IL 577

[Close]

BLAST of Bhi10G001167 vs. ExPASy TrEMBL
Match: A0A6J1D5S1 (cytochrome P450 81E8-like OS=Momordica charantia OX=3673 GN=LOC111017881 PE=3 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 5.7e-49
Identity = 96/123 (78.05%), Postives = 109/123 (88.62%), Query Frame = 0


Query: 1   CKIEGYDVPCNTIILINAWAVHRDSSIWEDAASFKPERHASGIEVDYSYKLIPFGSGRRA 60
           CKI GYDVP +TIILIN WA+HRD ++WEDA +FKPERH + I VD SYKL+PFGSGRR+
Sbjct: 371 CKIGGYDVPRDTIILINIWAIHRDPNLWEDATNFKPERHGNTIGVD-SYKLLPFGSGRRS 430

Query: 61  CPGVGMAQRVAGLTLASSIQSFEWERVNNSLIDMSEGKGLTMPKAQPLVAKCKPRLIMKV 120
           CPGVGMAQRV GLTLAS IQ FEWER++NSL+DM+EGKGLTMPKAQ LVAKCKPR IMK+
Sbjct: 431 CPGVGMAQRVVGLTLASLIQGFEWERMSNSLVDMTEGKGLTMPKAQHLVAKCKPRPIMKL 490

Query: 121 ILN 124
           ILN
Sbjct: 491 ILN 492

The following BLAST results are available for this feature:

BLAST of Bhi10G001167 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G66540.1	3.2e-36	56.52	Cytochrome P450 superfamily protein	[more]
AT1G66540.2	3.2e-36	56.52	Cytochrome P450 superfamily protein	[more]
AT4G37430.1	4.7e-35	54.92	cytochrome P450, family 91, subfamily A, polypeptide 2	[more]
AT4G37360.1	6.1e-35	55.74	cytochrome P450, family 81, subfamily D, polypeptide 2	[more]
AT5G67310.1	4.0e-34	54.47	cytochrome P450, family 81, subfamily G, polypeptide 1	[more]

BLAST of Bhi10G001167 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
W8JMU7	3.6e-40	59.52	Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1	[more]
O65790	6.6e-34	54.92	Cytochrome P450 81F1 OS=Arabidopsis thaliana OX=3702 GN=CYP81F1 PE=2 SV=2	[more]
Q9FG65	1.2e-32	55.28	Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1	[more]
Q9LHA1	6.8e-31	53.60	Cytochrome P450 81D11 OS=Arabidopsis thaliana OX=3702 GN=CYP81D11 PE=2 SV=1	[more]
Q6WNR0	2.2e-29	50.41	Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1	[more]

BLAST of Bhi10G001167 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038901939.1	1.0e-60	90.55	cytochrome P450 81Q32-like [Benincasa hispida]	[more]
XP_023512186.1	1.7e-50	81.15	isoflavone 2'-hydroxylase-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_02351...	[more]
XP_022985609.1	3.7e-50	80.33	isoflavone 2'-hydroxylase-like [Cucurbita maxima]	[more]
XP_022943757.1	1.1e-49	79.51	isoflavone 2'-hydroxylase-like [Cucurbita moschata]	[more]
XP_022943410.1	1.1e-49	79.51	isoflavone 2'-hydroxylase-like [Cucurbita moschata] >KAG7011036.1 Isoflavone 2'-...	[more]

BLAST of Bhi10G001167 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JDS1	1.8e-50	80.33	isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111483622 PE=3 ...	[more]
A0A6J1FXS5	5.2e-50	79.51	isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111448183 PE=...	[more]
A0A6J1FTX9	5.2e-50	79.51	isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111448413 PE=...	[more]
A0A6J1D5S6	1.2e-49	78.69	isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017884 PE...	[more]
A0A6J1D5S1	5.7e-49	78.05	cytochrome P450 81E8-like OS=Momordica charantia OX=3673 GN=LOC111017881 PE=3 SV...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 123..127
None	No IPR available	PANTHER	PTHR24298:SF836	CYTOCHROME P450, FAMILY 81, SUBFAMILY D, POLYPEPTIDE 2-RELATED	coord: 2..121
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 2..121
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 51..61 score: 54.73 coord: 17..41 score: 29.85 coord: 61..84 score: 30.65
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 1..118 e-value: 9.4E-30 score: 105.5
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 1..115
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 1..105 e-value: 3.7E-25 score: 88.6
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 54..63

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi10M001167	Bhi10M001167	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016709	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen