Bhi10G001080 (gene) Wax gourd (B227) v1

Overview


Name	Bhi10G001080
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Cysteine proteinase 1
Location	chr10: 33015984 .. 33017046 (+)
RNA-Seq Expression	Bhi10G001080
Synteny	Bhi10G001080

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAATTTATGGCAATGGACTACCCCCCAGGATCTAGTTCTGGTGACTTACAAGGCAGGTACCAGAAATGGATGAGTAAATACGGTCGACAATACAAGAGTAGAGAAGAGTGGGAGCGGAGATTCACAATTTATCAGTTGAATGTTCAGTACATTGACAACTTCAATTCTCTGGATCATTCATATACTCTAGCTGAAAATAACTTAGTAGATCTCACAAATGATGAGTTTAAGGAGACTTACTTGGGGTACAAAACTGATTGGCTTCCTGATACATGCTTTAGATATGGAAATATGGTACATTTGCCTACTAATGTTAACTGGAGAAAGGAAGGTGCAGTTACTCCAATAAATAATCAAGGCCAATGTGGTACGAACTCATAATACACTCGTTCATCTAACTAATCAGTAATCAGTATGAAAATGTTCTCTTTTCGTCTGATTTTGAGTTTTTTTTCCCTAACTACCCTACCCTCCTCTATCCATTGTAAGTCATCCCTTCGTATTCGAACAAACTCACCAGTTATGAATTTTTAACACAGGGAACTGTTGGGCATTCTCTGCAGTAGCAGCAGTGGAAGGCATCAACAAAATAAAAACAGGAAAATTGATGTCTCTATCAGAACAAGAGCTTGTGGACTGCGATGTGGCCTCGGGGAACCAGGGATGCAATGGTGGTTTCATGGACAAAGCATTTCAATTCATCAAGAAAACTAGACTCACTACAGAAACAGAATATCCATACAGGGGAATTGAATCTACATGTAATAAACAAAAAGTGAGAAACCACACCGTGGAAATAAGTGGATATGAAAAAGTGCCTGCCAATGATGAGAAAAGCTTAAAAGCAGTCGTTGCTAACCAGCCAGTCTCTTTAGCAATTGATGCAGGGGGATATGATTTTCAGTTCTATTCTGGTGGTGTCTTCTCAGGCAATTGTGGAAAACAACTCAATCATGGAGTGGCAATAGTTGGGTATAGGCAAGCTAGCAACAAATTGTTAAACCCCCGGTCTGCCCTGCTCCTTTTCAACTCACTTTCCATTAACAAGAAATTAAAATAA

mRNA sequence

ATGGAATTTATGGCAATGGACTACCCCCCAGGATCTAGTTCTGGTGACTTACAAGGCAGGTACCAGAAATGGATGAGTAAATACGGTCGACAATACAAGAGTAGAGAAGAGTGGGAGCGGAGATTCACAATTTATCAGTTGAATGTTCAGTACATTGACAACTTCAATTCTCTGGATCATTCATATACTCTAGCTGAAAATAACTTAGTAGATCTCACAAATGATGAGTTTAAGGAGACTTACTTGGGGTACAAAACTGATTGGCTTCCTGATACATGCTTTAGATATGGAAATATGGTACATTTGCCTACTAATGTTAACTGGAGAAAGGAAGGTGCAGTTACTCCAATAAATAATCAAGGCCAATGTGGGAACTGTTGGGCATTCTCTGCAGTAGCAGCAGTGGAAGGCATCAACAAAATAAAAACAGGAAAATTGATGTCTCTATCAGAACAAGAGCTTGTGGACTGCGATGTGGCCTCGGGGAACCAGGGATGCAATGGTGGTTTCATGGACAAAGCATTTCAATTCATCAAGAAAACTAGACTCACTACAGAAACAGAATATCCATACAGGGGAATTGAATCTACATGTAATAAACAAAAAGTGAGAAACCACACCGTGGAAATAAGTGGATATGAAAAAGTGCCTGCCAATGATGAGAAAAGCTTAAAAGCAGTCGTTGCTAACCAGCCAGTCTCTTTAGCAATTGATGCAGGGGGATATGATTTTCAGTTCTATTCTGGTGGTGTCTTCTCAGGCAATTGTGGAAAACAACTCAATCATGGAGTGGCAATAGTTGGGTATAGGCAAGCTAGCAACAAATTGTTAAACCCCCGGTCTGCCCTGCTCCTTTTCAACTCACTTTCCATTAACAAGAAATTAAAATAA

Coding sequence (CDS)

ATGGAATTTATGGCAATGGACTACCCCCCAGGATCTAGTTCTGGTGACTTACAAGGCAGGTACCAGAAATGGATGAGTAAATACGGTCGACAATACAAGAGTAGAGAAGAGTGGGAGCGGAGATTCACAATTTATCAGTTGAATGTTCAGTACATTGACAACTTCAATTCTCTGGATCATTCATATACTCTAGCTGAAAATAACTTAGTAGATCTCACAAATGATGAGTTTAAGGAGACTTACTTGGGGTACAAAACTGATTGGCTTCCTGATACATGCTTTAGATATGGAAATATGGTACATTTGCCTACTAATGTTAACTGGAGAAAGGAAGGTGCAGTTACTCCAATAAATAATCAAGGCCAATGTGGGAACTGTTGGGCATTCTCTGCAGTAGCAGCAGTGGAAGGCATCAACAAAATAAAAACAGGAAAATTGATGTCTCTATCAGAACAAGAGCTTGTGGACTGCGATGTGGCCTCGGGGAACCAGGGATGCAATGGTGGTTTCATGGACAAAGCATTTCAATTCATCAAGAAAACTAGACTCACTACAGAAACAGAATATCCATACAGGGGAATTGAATCTACATGTAATAAACAAAAAGTGAGAAACCACACCGTGGAAATAAGTGGATATGAAAAAGTGCCTGCCAATGATGAGAAAAGCTTAAAAGCAGTCGTTGCTAACCAGCCAGTCTCTTTAGCAATTGATGCAGGGGGATATGATTTTCAGTTCTATTCTGGTGGTGTCTTCTCAGGCAATTGTGGAAAACAACTCAATCATGGAGTGGCAATAGTTGGGTATAGGCAAGCTAGCAACAAATTGTTAAACCCCCGGTCTGCCCTGCTCCTTTTCAACTCACTTTCCATTAACAAGAAATTAAAATAA

Protein sequence

MEFMAMDYPPGSSSGDLQGRYQKWMSKYGRQYKSREEWERRFTIYQLNVQYIDNFNSLDHSYTLAENNLVDLTNDEFKETYLGYKTDWLPDTCFRYGNMVHLPTNVNWRKEGAVTPINNQGQCGNCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGFMDKAFQFIKKTRLTTETEYPYRGIESTCNKQKVRNHTVEISGYEKVPANDEKSLKAVVANQPVSLAIDAGGYDFQFYSGGVFSGNCGKQLNHGVAIVGYRQASNKLLNPRSALLLFNSLSINKKLK

Homology

[Close]

BLAST of Bhi10G001080 vs. TAIR 10
Match: AT1G06260.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 280.0 bits (715), Expect = 2.2e-75
Identity = 142/260 (54.62%), Postives = 178/260 (68.46%), Query Frame = 0


Query: 17  LQGRYQKWMSKYGRQYKSREEWERRFTIYQLNVQYIDNFNSLDHSYTLAENNLVDLTNDE 76
           L+ R++KW+  + + Y  R+EW  RF IYQ NVQ ID  NSL   + L +N   D+TN E
Sbjct: 39  LKQRFEKWLKTHSKLYGGRDEWMLRFGIYQSNVQLIDYINSLHLPFKLTDNRFADMTNSE 98

Query: 77  FKETYLGYKTDWL------PDTCFRYGNMVHLPTNVNWRKEGAVTPINNQGQCGNCWAFS 136
           FK  +LG  T  L         C   GN   +P  V+WR +GAVTPI NQG+CG CWAFS
Sbjct: 99  FKAHFLGLNTSSLRLHKKQRPVCDPAGN---VPDAVDWRTQGAVTPIRNQGKCGGCWAFS 158

Query: 137 AVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGFMDKAFQFIKKT-RLTTETEY 196
           AVAA+EGINKIKTG L+SLSEQ+L+DCDV + N+GC+GG M+ AF+FIK    L TET+Y
Sbjct: 159 AVAAIEGINKIKTGNLVSLSEQQLIDCDVGTYNKGCSGGLMETAFEFIKTNGGLATETDY 218

Query: 197 PYRGIESTCNKQKVRNHTVEISGYEKVPANDEKSLKAVVANQPVSLAIDAGGYDFQFYSG 256
           PY GIE TC+++K +N  V I GY+KV A +E SL+   A QPVS+ IDAGG+ FQ YS 
Sbjct: 219 PYTGIEGTCDQEKSKNKVVTIQGYQKV-AQNEASLQIAAAQQPVSVGIDAGGFIFQLYSS 278

Query: 257 GVFSGNCGKQLNHGVAIVGY 270
           GVF+  CG  LNHGV +VGY
Sbjct: 279 GVFTNYCGTNLNHGVTVVGY 294

[Close]

BLAST of Bhi10G001080 vs. TAIR 10
Match: AT5G50260.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 267.7 bits (683), Expect = 1.1e-71
Identity = 141/266 (53.01%), Postives = 176/266 (66.17%), Query Frame = 0


Query: 13  SSGDLQGRYQKWMSKYGRQYKSREEWERRFTIYQLNVQYIDNFNSLDHSYTLAENNLVDL 72
           S   L   Y++W S +    +S EE  +RF +++ NV++I   N  D SY L  N   D+
Sbjct: 30  SENSLWELYERWRSHH-TVARSLEEKAKRFNVFKHNVKHIHETNKKDKSYKLKLNKFGDM 89

Query: 73  TNDEFKETYLG--------YKTDWLPDTCFRYGNMVHLPTNVNWRKEGAVTPINNQGQCG 132
           T++EF+ TY G        ++ +      F Y N+  LPT+V+WRK GAVTP+ NQGQCG
Sbjct: 90  TSEEFRRTYAGSNIKHHRMFQGEKKATKSFMYANVNTLPTSVDWRKNGAVTPVKNQGQCG 149

Query: 133 NCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGFMDKAFQFIK-KTRL 192
           +CWAFS V AVEGIN+I+T KL SLSEQELVDCD  + NQGCNGG MD AF+FIK K  L
Sbjct: 150 SCWAFSTVVAVEGINQIRTKKLTSLSEQELVDCD-TNQNQGCNGGLMDLAFEFIKEKGGL 209

Query: 193 TTETEYPYRGIESTCNKQKVRNHTVEISGYEKVPANDEKSLKAVVANQPVSLAIDAGGYD 252
           T+E  YPY+  + TC+  K     V I G+E VP N E  L   VANQPVS+AIDAGG D
Sbjct: 210 TSELVYPYKASDETCDTNKENAPVVSIDGHEDVPKNSEDDLMKAVANQPVSVAIDAGGSD 269

Query: 253 FQFYSGGVFSGNCGKQLNHGVAIVGY 270
           FQFYS GVF+G CG +LNHGVA+VGY
Sbjct: 270 FQFYSEGVFTGRCGTELNHGVAVVGY 293

[Close]

BLAST of Bhi10G001080 vs. TAIR 10
Match: AT4G35350.1 (xylem cysteine peptidase 1 )

HSP 1 Score: 265.0 bits (676), Expect = 7.2e-71
Identity = 140/276 (50.72%), Postives = 182/276 (65.94%), Query Frame = 0


Query: 2   EFMAMDYPPG--SSSGDLQGRYQKWMSKYGRQYKSREEWERRFTIYQLNVQYIDNFNSLD 61
           +F  + Y P   +++  L   ++ WMS++ + YKS EE   RF +++ N+ +ID  N+  
Sbjct: 30  DFSIVGYTPEHLTNTDKLLELFESWMSEHSKAYKSVEEKVHRFEVFRENLMHIDQRNNEI 89

Query: 62  HSYTLAENNLVDLTNDEFKETYLG-----YKTDWLPDTCFRYGNMVHLPTNVNWRKEGAV 121
           +SY L  N   DLT++EFK  YLG     +     P   FRY ++  LP +V+WRK+GAV
Sbjct: 90  NSYWLGLNEFADLTHEEFKGRYLGLAKPQFSRKRQPSANFRYRDITDLPKSVDWRKKGAV 149

Query: 122 TPINNQGQCGNCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGFMDKA 181
            P+ +QGQCG+CWAFS VAAVEGIN+I TG L SLSEQEL+DCD  + N GCNGG MD A
Sbjct: 150 APVKDQGQCGSCWAFSTVAAVEGINQITTGNLSSLSEQELIDCD-TTFNSGCNGGLMDYA 209

Query: 182 FQFIKKT-RLTTETEYPYRGIESTCNKQKVRNHTVEISGYEKVPANDEKSLKAVVANQPV 241
           FQ+I  T  L  E +YPY   E  C +QK     V ISGYE VP ND++SL   +A+QPV
Sbjct: 210 FQYIISTGGLHKEDDYPYLMEEGICQEQKEDVERVTISGYEDVPENDDESLVKALAHQPV 269

Query: 242 SLAIDAGGYDFQFYSGGVFSGNCGKQLNHGVAIVGY 270
           S+AI+A G DFQFY GGVF+G CG  L+HGVA VGY
Sbjct: 270 SVAIEASGRDFQFYKGGVFNGKCGTDLDHGVAAVGY 304

[Close]

BLAST of Bhi10G001080 vs. TAIR 10
Match: AT5G45890.1 (senescence-associated gene 12 )

HSP 1 Score: 263.1 bits (671), Expect = 2.7e-70
Identity = 136/273 (49.82%), Postives = 183/273 (67.03%), Query Frame = 0


Query: 17  LQGRYQKWMSKYGRQYKSREEWERRFTIYQLNVQYIDNFNSL--DHSYTLAENNLVDLTN 76
           +Q R+ +WM+K+GR Y   +E   R+ +++ NV+ I++ NS+    ++ LA N   DLTN
Sbjct: 34  MQKRHIEWMTKHGRVYADVKEENNRYVVFKNNVERIEHLNSIPAGRTFKLAVNQFADLTN 93

Query: 77  DEFKETYLGYK----------TDWLPDTCFRYGNMVH--LPTNVNWRKEGAVTPINNQGQ 136
           DEF+  Y G+K          T   P   FRY N+    LP +V+WRK+GAVTPI NQG 
Sbjct: 94  DEFRSMYTGFKGVSALSSQSQTKMSP---FRYQNVSSGALPVSVDWRKKGAVTPIKNQGS 153

Query: 137 CGNCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGFMDKAFQFIKKT- 196
           CG CWAFSAVAA+EG  +IK GKL+SLSEQ+LVDCD  + + GC GG MD AF+ IK T 
Sbjct: 154 CGCCWAFSAVAAIEGATQIKKGKLISLSEQQLVDCD--TNDFGCEGGLMDTAFEHIKATG 213

Query: 197 RLTTETEYPYRGIESTCNKQKVRNHTVEISGYEKVPANDEKSLKAVVANQPVSLAIDAGG 256
            LTTE+ YPY+G ++TCN +K       I+GYE VP NDE++L   VA+QPVS+ I+ GG
Sbjct: 214 GLTTESNYPYKGEDATCNSKKTNPKATSITGYEDVPVNDEQALMKAVAHQPVSVGIEGGG 273

Query: 257 YDFQFYSGGVFSGNCGKQLNHGVAIVGYRQASN 275
           +DFQFYS GVF+G C   L+H V  +GY +++N
Sbjct: 274 FDFQFYSSGVFTGECTTYLDHAVTAIGYGESTN 301

[Close]

BLAST of Bhi10G001080 vs. TAIR 10
Match: AT3G48340.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 260.4 bits (664), Expect = 1.8e-69
Identity = 140/274 (51.09%), Postives = 173/274 (63.14%), Query Frame = 0


Query: 13  SSGDLQGRYQKWMSKYGRQYKSREEWERRFTIYQLNVQYIDNFNSLDHSYTLAENNLVDL 72
           S   L   Y +W S +    +S  E E+RF +++ NV ++ N N  + SY L  N   DL
Sbjct: 30  SEEGLSTLYDRWRSHHSVP-RSLNEREKRFNVFRHNVMHVHNTNKKNRSYKLKLNKFADL 89

Query: 73  TNDEFKETYLGYKTD----------WLPDTCFRYGNMVHLPTNVNWRKEGAVTPINNQGQ 132
           T +EFK  Y G                    + + N+  LP++V+WRK+GAVT I NQG+
Sbjct: 90  TINEFKNAYTGSNIKHHRMLQGPKRGSKQFMYDHENLSKLPSSVDWRKKGAVTEIKNQGK 149

Query: 133 CGNCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGFMDKAFQFIKKT- 192
           CG+CWAFS VAAVEGINKIKT KL+SLSEQELVDCD    N+GCNGG M+ AF+FIKK  
Sbjct: 150 CGSCWAFSTVAAVEGINKIKTNKLVSLSEQELVDCDTKQ-NEGCNGGLMEIAFEFIKKNG 209

Query: 193 RLTTETEYPYRGIESTCNKQKVRNHTVEISGYEKVPANDEKSLKAVVANQPVSLAIDAGG 252
            +TTE  YPY GI+  C+  K     V I G+E VP NDE +L   VANQPVS+AIDAG 
Sbjct: 210 GITTEDSYPYEGIDGKCDASKDNGVLVTIDGHEDVPENDENALLKAVANQPVSVAIDAGS 269

Query: 253 YDFQFYSGGVFSGNCGKQLNHGVAIVGYRQASNK 276
            DFQFYS GVF+G+CG +LNHGVA VGY     K
Sbjct: 270 SDFQFYSEGVFTGSCGTELNHGVAAVGYGSERGK 301

[Close]

BLAST of Bhi10G001080 vs. ExPASy Swiss-Prot
Match: P25803 (Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2)

HSP 1 Score: 269.2 bits (687), Expect = 5.4e-71
Identity = 139/267 (52.06%), Postives = 179/267 (67.04%), Query Frame = 0


Query: 12  SSSGDLQGRYQKWMSKYGRQYKSREEWERRFTIYQLNVQYIDNFNSLDHSYTLAENNLVD 71
           +S   L   Y++W S +    +S  E  +RF +++ N+ ++ N N +D  Y L  N   D
Sbjct: 31  ASEESLWDLYERWRSHH-TVSRSLGEKHKRFNVFKANLMHVHNTNKMDKPYKLKLNKFAD 90

Query: 72  LTNDEFKETYLGYKTDW--------LPDTCFRYGNMVHLPTNVNWRKEGAVTPINNQGQC 131
           +TN EF+ TY G K +           +  F Y  +V +P +V+WRK+GAVT + +QGQC
Sbjct: 91  MTNHEFRSTYAGSKVNHPRMFRGTPHENGAFMYEKVVSVPPSVDWRKKGAVTDVKDQGQC 150

Query: 132 GNCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGFMDKAFQFIK-KTR 191
           G+CWAFS V AVEGIN+IKT KL++LSEQELVDCD    NQGCNGG M+ AF+FIK K  
Sbjct: 151 GSCWAFSTVVAVEGINQIKTNKLVALSEQELVDCD-KEENQGCNGGLMESAFEFIKQKGG 210

Query: 192 LTTETEYPYRGIESTCNKQKVRNHTVEISGYEKVPANDEKSLKAVVANQPVSLAIDAGGY 251
           +TTE+ YPY+  E TC+  KV +  V I G+E VPANDE +L   VANQPVS+AIDAGG 
Sbjct: 211 ITTESNYPYKAQEGTCDASKVNDLAVSIDGHENVPANDEDALLKAVANQPVSVAIDAGGS 270

Query: 252 DFQFYSGGVFSGNCGKQLNHGVAIVGY 270
           DFQFYS GVF+G+C   LNHGVAIVGY
Sbjct: 271 DFQFYSEGVFTGDCSTDLNHGVAIVGY 295

[Close]

BLAST of Bhi10G001080 vs. ExPASy Swiss-Prot
Match: A2XQE8 (Senescence-specific cysteine protease SAG39 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_14861 PE=3 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 5.4e-71
Identity = 140/270 (51.85%), Postives = 184/270 (68.15%), Query Frame = 0


Query: 12  SSSGDLQGRYQKWMSKYGRQYKSREEWERRFTIYQLNVQYIDNFNSLDHSYTLAENNLVD 71
           S    +  R+++WM++YGR Y+   E  RRF +++ NV +I++FN+ +H++ L  N   D
Sbjct: 28  SDDAAMAARHERWMAQYGRVYRDDAEKARRFEVFKANVAFIESFNAGNHNFWLGVNQFAD 87

Query: 72  LTNDEFK--ETYLGY--KTDWLPDTCFRYG--NMVHLPTNVNWRKEGAVTPINNQGQCGN 131
           LTNDEF+  +T  G+   T  +P T FRY   N+  LP  V+WR +GAVTPI +QGQCG 
Sbjct: 88  LTNDEFRWTKTNKGFIPSTTRVP-TGFRYENVNIDALPATVDWRTKGAVTPIKDQGQCGC 147

Query: 132 CWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGFMDKAFQF-IKKTRLT 191
           CWAFSAVAA+EGI K+ TGKL+SLSEQELVDCDV   +QGC GG MD AF+F IK   LT
Sbjct: 148 CWAFSAVAAMEGIVKLSTGKLISLSEQELVDCDVHGEDQGCEGGLMDDAFKFIIKNGGLT 207

Query: 192 TETEYPYRGIESTCNKQKVRNHTVEISGYEKVPANDEKSLKAVVANQPVSLAIDAGGYDF 251
           TE+ YPY   +  C  + V N    I GYE VPAN+E +L   VANQPVS+A+D G   F
Sbjct: 208 TESNYPYAAADDKC--KSVSNSVASIKGYEDVPANNEAALMKAVANQPVSVAVDGGDMTF 267

Query: 252 QFYSGGVFSGNCGKQLNHGVAIVGYRQASN 275
           QFY GGV +G+CG  L+HG+  +GY +AS+
Sbjct: 268 QFYKGGVMTGSCGTDLDHGIVAIGYGKASD 294

[Close]

BLAST of Bhi10G001080 vs. ExPASy Swiss-Prot
Match: Q7XWK5 (Senescence-specific cysteine protease SAG39 OS=Oryza sativa subsp. japonica OX=39947 GN=SAG39 PE=2 SV=2)

HSP 1 Score: 269.2 bits (687), Expect = 5.4e-71
Identity = 140/270 (51.85%), Postives = 184/270 (68.15%), Query Frame = 0


Query: 12  SSSGDLQGRYQKWMSKYGRQYKSREEWERRFTIYQLNVQYIDNFNSLDHSYTLAENNLVD 71
           S    +  R+++WM++YGR Y+   E  RRF +++ NV +I++FN+ +H++ L  N   D
Sbjct: 28  SDDAAMAARHERWMAQYGRVYRDDAEKARRFEVFKANVAFIESFNAGNHNFWLGVNQFAD 87

Query: 72  LTNDEFK--ETYLGY--KTDWLPDTCFRYG--NMVHLPTNVNWRKEGAVTPINNQGQCGN 131
           LTNDEF+  +T  G+   T  +P T FRY   N+  LP  V+WR +GAVTPI +QGQCG 
Sbjct: 88  LTNDEFRWMKTNKGFIPSTTRVP-TGFRYENVNIDALPATVDWRTKGAVTPIKDQGQCGC 147

Query: 132 CWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGFMDKAFQF-IKKTRLT 191
           CWAFSAVAA+EGI K+ TGKL+SLSEQELVDCDV   +QGC GG MD AF+F IK   LT
Sbjct: 148 CWAFSAVAAMEGIVKLSTGKLISLSEQELVDCDVHGEDQGCEGGLMDDAFKFIIKNGGLT 207

Query: 192 TETEYPYRGIESTCNKQKVRNHTVEISGYEKVPANDEKSLKAVVANQPVSLAIDAGGYDF 251
           TE+ YPY   +  C  + V N    I GYE VPAN+E +L   VANQPVS+A+D G   F
Sbjct: 208 TESNYPYAAADDKC--KSVSNSVASIKGYEDVPANNEAALMKAVANQPVSVAVDGGDMTF 267

Query: 252 QFYSGGVFSGNCGKQLNHGVAIVGYRQASN 275
           QFY GGV +G+CG  L+HG+  +GY +AS+
Sbjct: 268 QFYKGGVMTGSCGTDLDHGIVAIGYGKASD 294

[Close]

BLAST of Bhi10G001080 vs. ExPASy Swiss-Prot
Match: Q9FGR9 (KDEL-tailed cysteine endopeptidase CEP1 OS=Arabidopsis thaliana OX=3702 GN=CEP1 PE=1 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 1.6e-70
Identity = 141/266 (53.01%), Postives = 176/266 (66.17%), Query Frame = 0


Query: 13  SSGDLQGRYQKWMSKYGRQYKSREEWERRFTIYQLNVQYIDNFNSLDHSYTLAENNLVDL 72
           S   L   Y++W S +    +S EE  +RF +++ NV++I   N  D SY L  N   D+
Sbjct: 30  SENSLWELYERWRSHH-TVARSLEEKAKRFNVFKHNVKHIHETNKKDKSYKLKLNKFGDM 89

Query: 73  TNDEFKETYLG--------YKTDWLPDTCFRYGNMVHLPTNVNWRKEGAVTPINNQGQCG 132
           T++EF+ TY G        ++ +      F Y N+  LPT+V+WRK GAVTP+ NQGQCG
Sbjct: 90  TSEEFRRTYAGSNIKHHRMFQGEKKATKSFMYANVNTLPTSVDWRKNGAVTPVKNQGQCG 149

Query: 133 NCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGFMDKAFQFIK-KTRL 192
           +CWAFS V AVEGIN+I+T KL SLSEQELVDCD  + NQGCNGG MD AF+FIK K  L
Sbjct: 150 SCWAFSTVVAVEGINQIRTKKLTSLSEQELVDCD-TNQNQGCNGGLMDLAFEFIKEKGGL 209

Query: 193 TTETEYPYRGIESTCNKQKVRNHTVEISGYEKVPANDEKSLKAVVANQPVSLAIDAGGYD 252
           T+E  YPY+  + TC+  K     V I G+E VP N E  L   VANQPVS+AIDAGG D
Sbjct: 210 TSELVYPYKASDETCDTNKENAPVVSIDGHEDVPKNSEDDLMKAVANQPVSVAIDAGGSD 269

Query: 253 FQFYSGGVFSGNCGKQLNHGVAIVGY 270
           FQFYS GVF+G CG +LNHGVA+VGY
Sbjct: 270 FQFYSEGVFTGRCGTELNHGVAVVGY 293

[Close]

BLAST of Bhi10G001080 vs. ExPASy Swiss-Prot
Match: P12412 (Vignain OS=Vigna mungo OX=3915 PE=1 SV=1)

HSP 1 Score: 266.2 bits (679), Expect = 4.6e-70
Identity = 138/266 (51.88%), Postives = 175/266 (65.79%), Query Frame = 0


Query: 13  SSGDLQGRYQKWMSKYGRQYKSREEWERRFTIYQLNVQYIDNFNSLDHSYTLAENNLVDL 72
           S   L   Y++W S +    +S  E  +RF +++ NV ++ N N +D  Y L  N   D+
Sbjct: 32  SEESLWDLYERWRSHH-TVSRSLGEKHKRFNVFKANVMHVHNTNKMDKPYKLKLNKFADM 91

Query: 73  TNDEFKETYLGYKTDWLP--------DTCFRYGNMVHLPTNVNWRKEGAVTPINNQGQCG 132
           TN EF+ TY G K +              F Y  +  +P +V+WRK+GAVT + +QGQCG
Sbjct: 92  TNHEFRSTYAGSKVNHHKMFRGSQHGSGTFMYEKVGSVPASVDWRKKGAVTDVKDQGQCG 151

Query: 133 NCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGFMDKAFQFIK-KTRL 192
           +CWAFS + AVEGIN+IKT KL+SLSEQELVDCD    NQGCNGG M+ AF+FIK K  +
Sbjct: 152 SCWAFSTIVAVEGINQIKTNKLVSLSEQELVDCD-KEENQGCNGGLMESAFEFIKQKGGI 211

Query: 193 TTETEYPYRGIESTCNKQKVRNHTVEISGYEKVPANDEKSLKAVVANQPVSLAIDAGGYD 252
           TTE+ YPY   E TC++ KV +  V I G+E VP NDE +L   VANQPVS+AIDAGG D
Sbjct: 212 TTESNYPYTAQEGTCDESKVNDLAVSIDGHENVPVNDENALLKAVANQPVSVAIDAGGSD 271

Query: 253 FQFYSGGVFSGNCGKQLNHGVAIVGY 270
           FQFYS GVF+G+C   LNHGVAIVGY
Sbjct: 272 FQFYSEGVFTGDCNTDLNHGVAIVGY 295

[Close]

BLAST of Bhi10G001080 vs. NCBI nr
Match: XP_038902648.1 (ervatamin-B-like [Benincasa hispida])

HSP 1 Score: 569.3 bits (1466), Expect = 1.9e-158
Identity = 272/273 (99.63%), Postives = 273/273 (100.00%), Query Frame = 0


Query: 1   MEFMAMDYPPGSSSGDLQGRYQKWMSKYGRQYKSREEWERRFTIYQLNVQYIDNFNSLDH 60
           MEFMAMDYPPGSSSGDLQGRYQKWMSKYGRQYKSREEWERRFTIYQLNVQYIDNFNSLDH
Sbjct: 25  MEFMAMDYPPGSSSGDLQGRYQKWMSKYGRQYKSREEWERRFTIYQLNVQYIDNFNSLDH 84

Query: 61  SYTLAENNLVDLTNDEFKETYLGYKTDWLPDTCFRYGNMVHLPTNVNWRKEGAVTPINNQ 120
           SYTLAENNLVDLTNDEFKETYLGYKTDWLPDTCFRYGNMVHLPTNVNWRKEGAVTPINNQ
Sbjct: 85  SYTLAENNLVDLTNDEFKETYLGYKTDWLPDTCFRYGNMVHLPTNVNWRKEGAVTPINNQ 144

Query: 121 GQCGNCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGFMDKAFQFIKK 180
           GQCGNCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGFMDKAFQFIKK
Sbjct: 145 GQCGNCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGFMDKAFQFIKK 204

Query: 181 TRLTTETEYPYRGIESTCNKQKVRNHTVEISGYEKVPANDEKSLKAVVANQPVSLAIDAG 240
           TRLTTETEYPYRGIESTCNKQKVRNHTVEISGYEKVPANDEKSLKAVVANQPVSLAIDAG
Sbjct: 205 TRLTTETEYPYRGIESTCNKQKVRNHTVEISGYEKVPANDEKSLKAVVANQPVSLAIDAG 264

Query: 241 GYDFQFYSGGVFSGNCGKQLNHGVAIVGYRQAS 274
           GYDFQFYSGGVFSGNCGKQLNHGVAIVGYRQA+
Sbjct: 265 GYDFQFYSGGVFSGNCGKQLNHGVAIVGYRQAT 297

[Close]

BLAST of Bhi10G001080 vs. NCBI nr
Match: XP_038902939.1 (ervatamin-B [Benincasa hispida])

HSP 1 Score: 552.7 bits (1423), Expect = 1.9e-153
Identity = 265/275 (96.36%), Postives = 267/275 (97.09%), Query Frame = 0


Query: 1   MEFMAMDYPPGSSSGDLQGRYQKWMSKYGRQYKSREEWERRFTIYQLNVQYIDNFNSLDH 60
           M FMAMDYPPGSSSGDLQGRYQKWMSKYGRQYKSREEWERRFTIYQLNVQYIDNFNSLDH
Sbjct: 25  MVFMAMDYPPGSSSGDLQGRYQKWMSKYGRQYKSREEWERRFTIYQLNVQYIDNFNSLDH 84

Query: 61  SYTLAENNLVDLTNDEFKETYLGYKTDWLPDTCFRYGNMVHLPTNVNWRKEGAVTPINNQ 120
           SYTLAENN  DLTNDEFKETYLGYKTDWLPDTCFRYGNMV LPTNVNWRKEGAVTPI NQ
Sbjct: 85  SYTLAENNFADLTNDEFKETYLGYKTDWLPDTCFRYGNMVDLPTNVNWRKEGAVTPIKNQ 144

Query: 121 GQCGNCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGFMDKAFQFIKK 180
           GQCG+CWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGFMDKAFQFIKK
Sbjct: 145 GQCGSCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGFMDKAFQFIKK 204

Query: 181 TRLTTETEYPYRGIESTCNKQKVRNHTVEISGYEKVPANDEKSLKAVVANQPVSLAIDAG 240
           T LTTETEYPYRGIESTCNKQKVRNHTVEISGYEKVPANDEKSLKA VANQPVS+AIDAG
Sbjct: 205 TGLTTETEYPYRGIESTCNKQKVRNHTVEISGYEKVPANDEKSLKAAVANQPVSVAIDAG 264

Query: 241 GYDFQFYSGGVFSGNCGKQLNHGVAIVGYRQASNK 276
           GYDFQFYSGGVFSGNCGKQLNHGVAIVGY QASNK
Sbjct: 265 GYDFQFYSGGVFSGNCGKQLNHGVAIVGYGQASNK 299

[Close]

BLAST of Bhi10G001080 vs. NCBI nr
Match: AKO60151.1 (cysteine proteinase 1, partial [Citrullus lanatus])

HSP 1 Score: 498.4 bits (1282), Expect = 4.2e-137
Identity = 239/275 (86.91%), Postives = 253/275 (92.00%), Query Frame = 0


Query: 1   MEFMAMDYPPGSSSGDLQGRYQKWMSKYGRQYKSREEWERRFTIYQLNVQYIDNFNSLDH 60
           M  M MDY PGSSSGDLQ RYQKWMSKYGR+YKSREEWE+RF IYQLNVQYIDNFNSL+H
Sbjct: 1   MASMEMDYRPGSSSGDLQDRYQKWMSKYGREYKSREEWEQRFNIYQLNVQYIDNFNSLNH 60

Query: 61  SYTLAENNLVDLTNDEFKETYLGYKTDWLPDTCFRYGNMVHLPTNVNWRKEGAVTPINNQ 120
           SYTLAEN+  DLTNDEFK TYLG+KTDWLPDT FRYGNMV+LPTNV+WRKE AVTP+ +Q
Sbjct: 61  SYTLAENSFADLTNDEFKTTYLGFKTDWLPDTWFRYGNMVNLPTNVDWRKENAVTPVKDQ 120

Query: 121 GQCGNCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGFMDKAFQFIKK 180
           GQCG+CWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGG+M KAF+FIKK
Sbjct: 121 GQCGSCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGYMYKAFEFIKK 180

Query: 181 TRLTTETEYPYRGIESTCNKQKVRNHTVEISGYEKVPANDEKSLKAVVANQPVSLAIDAG 240
           T LTTE EYPYRGIES CNKQKVR  TV ISGYEKVP NDEKSLKA VANQPVS+AIDAG
Sbjct: 181 TGLTTEIEYPYRGIESVCNKQKVRYRTVTISGYEKVPVNDEKSLKAAVANQPVSVAIDAG 240

Query: 241 GYDFQFYSGGVFSGNCGKQLNHGVAIVGYRQASNK 276
           GYDFQFYSGGVFSGNCGKQLNHGVAIVGY +ASNK
Sbjct: 241 GYDFQFYSGGVFSGNCGKQLNHGVAIVGYGEASNK 275

[Close]

BLAST of Bhi10G001080 vs. NCBI nr
Match: KGN62053.1 (hypothetical protein Csa_006706 [Cucumis sativus])

HSP 1 Score: 455.7 bits (1171), Expect = 3.1e-124
Identity = 219/273 (80.22%), Postives = 241/273 (88.28%), Query Frame = 0


Query: 4   MAMDYPPGSS-SGDLQGRYQKWMSKYGRQYKSREEWERRFTIYQLNVQYIDNFNSLDHSY 63
           MAMDY  GSS S D+Q RYQKWM KYGRQYKSREEWERRFTIYQ NVQYIDNFNS++HS+
Sbjct: 24  MAMDYSLGSSCSSDIQDRYQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSH 83

Query: 64  TLAENNLVDLTNDEFKETYLGYKTDWLPDTCFRYGNMVHLPTNVNWRKEGAVTPINNQGQ 123
           TLAENN  DLTN+EFK TYLGYKT  +PDTCFRYGNMV+LPTNV+WR+EGAVTPI NQGQ
Sbjct: 84  TLAENNFADLTNEEFKATYLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQ 143

Query: 124 CGNCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGFMDKAFQFIKKTR 183
           CG+CWAFSAVAAVEGINKIK GKL+SLSEQELVDCDV SGNQGCNGG+M KAF+FIK+T 
Sbjct: 144 CGSCWAFSAVAAVEGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTG 203

Query: 184 LTTETEYPYRGIESTCNKQKVRNHTVEISGYEKVPANDEKSLKAVVANQPVSLAIDAGGY 243
           LTTE EYPY+G ES CN+QK +   V ISGYEKVP NDEKSLKA VANQPVS+AIDA G 
Sbjct: 204 LTTEIEYPYQGAESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGN 263

Query: 244 DFQFYSGGVFSGNCGKQLNHGVAIVGYRQASNK 276
           +FQFYSGG+FSGNCG QLNHGVAIVGY + SN+
Sbjct: 264 NFQFYSGGIFSGNCGNQLNHGVAIVGYGETSNQ 296

[Close]

BLAST of Bhi10G001080 vs. NCBI nr
Match: XP_004148072.2 (ervatamin-B [Cucumis sativus])

HSP 1 Score: 455.7 bits (1171), Expect = 3.1e-124
Identity = 219/273 (80.22%), Postives = 241/273 (88.28%), Query Frame = 0


Query: 4   MAMDYPPGSS-SGDLQGRYQKWMSKYGRQYKSREEWERRFTIYQLNVQYIDNFNSLDHSY 63
           MAMDY  GSS S D+Q RYQKWM KYGRQYKSREEWERRFTIYQ NVQYIDNFNS++HS+
Sbjct: 40  MAMDYSLGSSCSSDIQDRYQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSH 99

Query: 64  TLAENNLVDLTNDEFKETYLGYKTDWLPDTCFRYGNMVHLPTNVNWRKEGAVTPINNQGQ 123
           TLAENN  DLTN+EFK TYLGYKT  +PDTCFRYGNMV+LPTNV+WR+EGAVTPI NQGQ
Sbjct: 100 TLAENNFADLTNEEFKATYLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQ 159

Query: 124 CGNCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGFMDKAFQFIKKTR 183
           CG+CWAFSAVAAVEGINKIK GKL+SLSEQELVDCDV SGNQGCNGG+M KAF+FIK+T 
Sbjct: 160 CGSCWAFSAVAAVEGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTG 219

Query: 184 LTTETEYPYRGIESTCNKQKVRNHTVEISGYEKVPANDEKSLKAVVANQPVSLAIDAGGY 243
           LTTE EYPY+G ES CN+QK +   V ISGYEKVP NDEKSLKA VANQPVS+AIDA G 
Sbjct: 220 LTTEIEYPYQGAESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGN 279

Query: 244 DFQFYSGGVFSGNCGKQLNHGVAIVGYRQASNK 276
           +FQFYSGG+FSGNCG QLNHGVAIVGY + SN+
Sbjct: 280 NFQFYSGGIFSGNCGNQLNHGVAIVGYGETSNQ 312

[Close]

BLAST of Bhi10G001080 vs. ExPASy TrEMBL
Match: A0A384S0D9 (Cysteine proteinase 1 (Fragment) OS=Citrullus lanatus OX=3654 GN=ClCP1 PE=2 SV=1)

HSP 1 Score: 498.4 bits (1282), Expect = 2.0e-137
Identity = 239/275 (86.91%), Postives = 253/275 (92.00%), Query Frame = 0


Query: 1   MEFMAMDYPPGSSSGDLQGRYQKWMSKYGRQYKSREEWERRFTIYQLNVQYIDNFNSLDH 60
           M  M MDY PGSSSGDLQ RYQKWMSKYGR+YKSREEWE+RF IYQLNVQYIDNFNSL+H
Sbjct: 1   MASMEMDYRPGSSSGDLQDRYQKWMSKYGREYKSREEWEQRFNIYQLNVQYIDNFNSLNH 60

Query: 61  SYTLAENNLVDLTNDEFKETYLGYKTDWLPDTCFRYGNMVHLPTNVNWRKEGAVTPINNQ 120
           SYTLAEN+  DLTNDEFK TYLG+KTDWLPDT FRYGNMV+LPTNV+WRKE AVTP+ +Q
Sbjct: 61  SYTLAENSFADLTNDEFKTTYLGFKTDWLPDTWFRYGNMVNLPTNVDWRKENAVTPVKDQ 120

Query: 121 GQCGNCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGFMDKAFQFIKK 180
           GQCG+CWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGG+M KAF+FIKK
Sbjct: 121 GQCGSCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGYMYKAFEFIKK 180

Query: 181 TRLTTETEYPYRGIESTCNKQKVRNHTVEISGYEKVPANDEKSLKAVVANQPVSLAIDAG 240
           T LTTE EYPYRGIES CNKQKVR  TV ISGYEKVP NDEKSLKA VANQPVS+AIDAG
Sbjct: 181 TGLTTEIEYPYRGIESVCNKQKVRYRTVTISGYEKVPVNDEKSLKAAVANQPVSVAIDAG 240

Query: 241 GYDFQFYSGGVFSGNCGKQLNHGVAIVGYRQASNK 276
           GYDFQFYSGGVFSGNCGKQLNHGVAIVGY +ASNK
Sbjct: 241 GYDFQFYSGGVFSGNCGKQLNHGVAIVGYGEASNK 275

[Close]

BLAST of Bhi10G001080 vs. ExPASy TrEMBL
Match: A0A0A0LJV6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G292830 PE=3 SV=1)

HSP 1 Score: 455.7 bits (1171), Expect = 1.5e-124
Identity = 219/273 (80.22%), Postives = 241/273 (88.28%), Query Frame = 0


Query: 4   MAMDYPPGSS-SGDLQGRYQKWMSKYGRQYKSREEWERRFTIYQLNVQYIDNFNSLDHSY 63
           MAMDY  GSS S D+Q RYQKWM KYGRQYKSREEWERRFTIYQ NVQYIDNFNS++HS+
Sbjct: 24  MAMDYSLGSSCSSDIQDRYQKWMDKYGRQYKSREEWERRFTIYQANVQYIDNFNSMNHSH 83

Query: 64  TLAENNLVDLTNDEFKETYLGYKTDWLPDTCFRYGNMVHLPTNVNWRKEGAVTPINNQGQ 123
           TLAENN  DLTN+EFK TYLGYKT  +PDTCFRYGNMV+LPTNV+WR+EGAVTPI NQGQ
Sbjct: 84  TLAENNFADLTNEEFKATYLGYKTVSIPDTCFRYGNMVNLPTNVDWRQEGAVTPIKNQGQ 143

Query: 124 CGNCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGFMDKAFQFIKKTR 183
           CG+CWAFSAVAAVEGINKIK GKL+SLSEQELVDCDV SGNQGCNGG+M KAF+FIK+T 
Sbjct: 144 CGSCWAFSAVAAVEGINKIKAGKLISLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKRTG 203

Query: 184 LTTETEYPYRGIESTCNKQKVRNHTVEISGYEKVPANDEKSLKAVVANQPVSLAIDAGGY 243
           LTTE EYPY+G ES CN+QK +   V ISGYEKVP NDEKSLKA VANQPVS+AIDA G 
Sbjct: 204 LTTEIEYPYQGAESACNEQKEKYQFVSISGYEKVPVNDEKSLKAAVANQPVSVAIDAEGN 263

Query: 244 DFQFYSGGVFSGNCGKQLNHGVAIVGYRQASNK 276
           +FQFYSGG+FSGNCG QLNHGVAIVGY + SN+
Sbjct: 264 NFQFYSGGIFSGNCGNQLNHGVAIVGYGETSNQ 296

[Close]

BLAST of Bhi10G001080 vs. ExPASy TrEMBL
Match: A0A5A7SQK0 (Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold111G001250 PE=3 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 1.7e-123
Identity = 222/275 (80.73%), Postives = 240/275 (87.27%), Query Frame = 0


Query: 4   MAMDYPPGSS-SGDLQGRYQKWMSKYGRQYKSREEWERRFTIYQLNVQYIDNFNSLDHSY 63
           MAMDYP GSS SG+LQ RYQKWM KYGRQYKSREEWE+RFTIYQ NVQYIDNFNSL+HSY
Sbjct: 28  MAMDYPSGSSNSGELQDRYQKWMDKYGRQYKSREEWEQRFTIYQANVQYIDNFNSLNHSY 87

Query: 64  TLAENNLVDLTNDEFKETYLGYKTDWLP--DTCFRYGNMVHLPTNVNWRKEGAVTPINNQ 123
           TLAENN  DLTN+EF  TYLGY+T  LP  DT FRYGNMV+LPTNV+WRKEGAVTPI NQ
Sbjct: 88  TLAENNFTDLTNEEFMATYLGYETVSLPDTDTFFRYGNMVNLPTNVDWRKEGAVTPIKNQ 147

Query: 124 GQCGNCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGFMDKAFQFIKK 183
           GQCG+CWAFSAVAAVEGINKIK GKLMSLSEQELVDCDV SGNQGCNGG+M KAF+FIKK
Sbjct: 148 GQCGSCWAFSAVAAVEGINKIKAGKLMSLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKK 207

Query: 184 TRLTTETEYPYRGIESTCNKQKVRNHTVEISGYEKVPANDEKSLKAVVANQPVSLAIDAG 243
           T LTTE EYPY    S C+KQK +  +V ISGYEKVP NDEKSL+A VA QPVS+AIDAG
Sbjct: 208 TGLTTELEYPYTATRSACDKQKEKYQSVSISGYEKVPVNDEKSLQAAVAKQPVSVAIDAG 267

Query: 244 GYDFQFYSGGVFSGNCGKQLNHGVAIVGYRQASNK 276
           G DFQFYSGG+FSGNCGKQLNHGVAIVGY + SN+
Sbjct: 268 GNDFQFYSGGIFSGNCGKQLNHGVAIVGYGEDSNQ 302

[Close]

BLAST of Bhi10G001080 vs. ExPASy TrEMBL
Match: A0A1S3C828 (ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103497881 PE=3 SV=1)

HSP 1 Score: 452.2 bits (1162), Expect = 1.7e-123
Identity = 222/275 (80.73%), Postives = 240/275 (87.27%), Query Frame = 0


Query: 4   MAMDYPPGSS-SGDLQGRYQKWMSKYGRQYKSREEWERRFTIYQLNVQYIDNFNSLDHSY 63
           MAMDYP GSS SG+LQ RYQKWM KYGRQYKSREEWE+RFTIYQ NVQYIDNFNSL+HSY
Sbjct: 28  MAMDYPSGSSNSGELQDRYQKWMDKYGRQYKSREEWEQRFTIYQANVQYIDNFNSLNHSY 87

Query: 64  TLAENNLVDLTNDEFKETYLGYKTDWLP--DTCFRYGNMVHLPTNVNWRKEGAVTPINNQ 123
           TLAENN  DLTN+EF  TYLGY+T  LP  DT FRYGNMV+LPTNV+WRKEGAVTPI NQ
Sbjct: 88  TLAENNFTDLTNEEFMATYLGYETVSLPDTDTFFRYGNMVNLPTNVDWRKEGAVTPIKNQ 147

Query: 124 GQCGNCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGFMDKAFQFIKK 183
           GQCG+CWAFSAVAAVEGINKIK GKLMSLSEQELVDCDV SGNQGCNGG+M KAF+FIKK
Sbjct: 148 GQCGSCWAFSAVAAVEGINKIKAGKLMSLSEQELVDCDVTSGNQGCNGGYMYKAFEFIKK 207

Query: 184 TRLTTETEYPYRGIESTCNKQKVRNHTVEISGYEKVPANDEKSLKAVVANQPVSLAIDAG 243
           T LTTE EYPY    S C+KQK +  +V ISGYEKVP NDEKSL+A VA QPVS+AIDAG
Sbjct: 208 TGLTTELEYPYTATRSACDKQKEKYQSVSISGYEKVPVNDEKSLQAAVAKQPVSVAIDAG 267

Query: 244 GYDFQFYSGGVFSGNCGKQLNHGVAIVGYRQASNK 276
           G DFQFYSGG+FSGNCGKQLNHGVAIVGY + SN+
Sbjct: 268 GNDFQFYSGGIFSGNCGKQLNHGVAIVGYGEDSNQ 302

[Close]

BLAST of Bhi10G001080 vs. ExPASy TrEMBL
Match: A0A6J1CH04 (ervatamin-B OS=Momordica charantia OX=3673 GN=LOC111011342 PE=3 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 3.1e-122
Identity = 212/275 (77.09%), Postives = 237/275 (86.18%), Query Frame = 0


Query: 1   MEFMAMDYPPGSSSGDLQGRYQKWMSKYGRQYKSREEWERRFTIYQLNVQYIDNFNSLDH 60
           M  +A D PPG  S D++ RYQKW+ KYGR+YKS EE E+RF IYQ NVQYID FNSL+ 
Sbjct: 25  MASVAEDNPPGDGSDDMRDRYQKWIDKYGREYKSGEEREKRFPIYQSNVQYIDYFNSLNR 84

Query: 61  SYTLAENNLVDLTNDEFKETYLGYKTDWLPDTCFRYGNMVHLPTNVNWRKEGAVTPINNQ 120
           SYTLA+N   DLTNDEFK TYLGY TDW PDTCF+YGN+V+LPTNV+WRKEGAVTPI +Q
Sbjct: 85  SYTLADNMFADLTNDEFKTTYLGYLTDWSPDTCFKYGNIVNLPTNVDWRKEGAVTPIKDQ 144

Query: 121 GQCGNCWAFSAVAAVEGINKIKTGKLMSLSEQELVDCDVASGNQGCNGGFMDKAFQFIKK 180
           GQCG+CWAFSAVAAVEGI KIKTGKL+SLSEQEL+DCDV SGNQGC+GGFM KAF+FIKK
Sbjct: 145 GQCGSCWAFSAVAAVEGITKIKTGKLVSLSEQELLDCDVISGNQGCSGGFMPKAFEFIKK 204

Query: 181 TRLTTETEYPYRGIESTCNKQKVRNHTVEISGYEKVPANDEKSLKAVVANQPVSLAIDAG 240
             +TTE EYPYRG+E+ CNKQKVR H+  ISGYEKVPANDEKSLKA VANQPVS+AIDAG
Sbjct: 205 IGITTEKEYPYRGVENVCNKQKVRYHSATISGYEKVPANDEKSLKAAVANQPVSVAIDAG 264

Query: 241 GYDFQFYSGGVFSGNCGKQLNHGVAIVGYRQASNK 276
           GYDFQFYSGG+FSGNCGKQLNHGV IVGY +   K
Sbjct: 265 GYDFQFYSGGIFSGNCGKQLNHGVTIVGYGEDVGK 299

The following BLAST results are available for this feature:

BLAST of Bhi10G001080 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G06260.1	2.2e-75	54.62	Cysteine proteinases superfamily protein	[more]
AT5G50260.1	1.1e-71	53.01	Cysteine proteinases superfamily protein	[more]
AT4G35350.1	7.2e-71	50.72	xylem cysteine peptidase 1	[more]
AT5G45890.1	2.7e-70	49.82	senescence-associated gene 12	[more]
AT3G48340.1	1.8e-69	51.09	Cysteine proteinases superfamily protein	[more]

BLAST of Bhi10G001080 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P25803	5.4e-71	52.06	Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2	[more]
A2XQE8	5.4e-71	51.85	Senescence-specific cysteine protease SAG39 OS=Oryza sativa subsp. indica OX=399...	[more]
Q7XWK5	5.4e-71	51.85	Senescence-specific cysteine protease SAG39 OS=Oryza sativa subsp. japonica OX=3...	[more]
Q9FGR9	1.6e-70	53.01	KDEL-tailed cysteine endopeptidase CEP1 OS=Arabidopsis thaliana OX=3702 GN=CEP1 ...	[more]
P12412	4.6e-70	51.88	Vignain OS=Vigna mungo OX=3915 PE=1 SV=1	[more]

BLAST of Bhi10G001080 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038902648.1	1.9e-158	99.63	ervatamin-B-like [Benincasa hispida]	[more]
XP_038902939.1	1.9e-153	96.36	ervatamin-B [Benincasa hispida]	[more]
AKO60151.1	4.2e-137	86.91	cysteine proteinase 1, partial [Citrullus lanatus]	[more]
KGN62053.1	3.1e-124	80.22	hypothetical protein Csa_006706 [Cucumis sativus]	[more]
XP_004148072.2	3.1e-124	80.22	ervatamin-B [Cucumis sativus]	[more]

BLAST of Bhi10G001080 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A384S0D9	2.0e-137	86.91	Cysteine proteinase 1 (Fragment) OS=Citrullus lanatus OX=3654 GN=ClCP1 PE=2 SV=1	[more]
A0A0A0LJV6	1.5e-124	80.22	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G292830 PE=3 SV=1	[more]
A0A5A7SQK0	1.7e-123	80.73	Ervatamin-B-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold111G001...	[more]
A0A1S3C828	1.7e-123	80.73	ervatamin-B-like OS=Cucumis melo OX=3656 GN=LOC103497881 PE=3 SV=1	[more]
A0A6J1CH04	3.1e-122	77.09	ervatamin-B OS=Momordica charantia OX=3673 GN=LOC111011342 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000668	Peptidase C1A, papain C-terminal	PRINTS	PR00705	PAPAIN	coord: 120..135 score: 62.08 coord: 262..272 score: 46.85
IPR000668	Peptidase C1A, papain C-terminal	SMART	SM00645	pept_c1	coord: 102..291 e-value: 2.0E-71 score: 253.3
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 102..273 e-value: 3.1E-59 score: 200.7
IPR013201	Cathepsin propeptide inhibitor domain (I29)	SMART	SM00848	Inhibitor_I29_2	coord: 21..77 e-value: 2.1E-18 score: 77.2
IPR013201	Cathepsin propeptide inhibitor domain (I29)	PFAM	PF08246	Inhibitor_I29	coord: 21..77 e-value: 6.7E-12 score: 45.7
None	No IPR available	GENE3D	3.90.70.10	Cysteine proteinases	coord: 7..291 e-value: 7.0E-90 score: 303.7
None	No IPR available	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 14..272
None	No IPR available	PANTHER	PTHR12411:SF796	ERVATAMIN-B-LIKE	coord: 14..272
IPR000169	Cysteine peptidase, cysteine active site	PROSITE	PS00139	THIOL_PROTEASE_CYS	coord: 120..131
IPR039417	Papain-like cysteine endopeptidase	CDD	cd02248	Peptidase_C1A	coord: 103..269 e-value: 5.53084E-76 score: 229.048
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 19..272

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi10M001080	Bhi10M001080	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051603	proteolysis involved in cellular protein catabolic process
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005615	extracellular space
cellular_component	GO:0005764	lysosome
molecular_function	GO:0004197	cysteine-type endopeptidase activity
molecular_function	GO:0008234	cysteine-type peptidase activity