Bhi10G000939 (gene) Wax gourd (B227) v1

Overview
NameBhi10G000939
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptionindole-3-acetic acid-amido synthetase GH3.6-like
Locationchr10: 26675406 .. 26677200 (-)
RNA-Seq ExpressionBhi10G000939
SyntenyBhi10G000939
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCGCATATTGAAGAGAATTGGTTGGATGAGAAAGATATGGAAGCATTACAACACATTGAAGATTTCACAAGCAAAGCCGATGAAATTCAGCGACAAATTTTAACTGAAATTCTGTCAACAAATGCTGATGTTGAATACTTGCAACGACATGGCCTTGATGGCTCTACTGATGCTTCAACCTTCAAGAAATCCAGCGGAAGGAATTTTTTTTTGGTAGCTAGCGAGTGGCTCGCGTGAGGAAGATGGGGATGGATTCTGTCTTTTTATTTAAAATAATAATAATAATAATAATAACTAAAAAGAAAAAATATAATTACACTTAAATCATTTTCTTAATCCACTTTATACTATTTATTTCTTTTCTTTTTCAAAAGAAAATTAATTCCTGTCAATTTGAAAATTCAATAATTTTCACGTCAACACTTAAATTTCCACACTTATACTTCAAATCCAAAATTCTAAAAATATCCTCCAATAACAAAATTACTGAAATCATATAAAAAGTGAAAATTCGAGGTGTTACATAAGTTTAGAACAAAAGCTGGTATGGTTGCTGGTACCGCAATTACAAGTTTGTGCAAAAGATCATCAAATCTCAAAAGTGAACCAACTGGGAATAACACAAGCCCTGATGATATCATTCTCTGTACAGACTCATTCCAAAGCTTATACTGTCAACTTCTTTGTGGGCTCTACCAAAACGATGTAGTTTTCCGAATTGGTGCTCTTTTTGCTTCTACGTTGATTCACATTTTTTAGTTCCTTGAAAACCATTGGGTTGTTTTGGCTAATGATATACGAACAGGAAGTATTAACCCCAAAATTACAGACTCATCAGTGAGGGAATCAGTTATGAAGATTGTTGTAAAACCCAATCCTGAAGTTGCTGATTTGATTGAACATGAATGTAGCAAAGGAAGCTGGGAAGGAATTATTAAAAGATTGTGGCCAAACACCAAATACATTAATGCTATTGCGACAGGAAGTATGTCACAATACATTCCATTGCTAAATTACTACACCAATAATCTCCCTATTGTTTCTGACCATTATGGTTCTTCAGAATGTTTCCTTGGCTTAAACTTAGATCCATTGTGTGACCCTTATGAAATCTCCTACACTTTGATACCCACCATGGCGTATTTCGAGTTCTTGCCAATCGATAGGACAAATCCCCATGGAGGAAGAAGCCAAGAATTAGTTGATCTTGTTGATGTCAAGTTAGGACAAGAATATGAGCTAGTCATCACAACTTTTGCAGGTAAGTCTTGCTCTCAGTATTGAATTCTTAATCAAATTCTAAACTTCAAAAACAAAAATTTTAAAAAAATATAAAAACAAAAAAATAAGGAAAATTATTTACACAAATAGAAAAATTTAATTATAGTTGTGATAGATATTATTATCTATATCTATTACAAAAAGATGTTGATAAAAGTCTATTGAAGGATTGTTAGAGGACATTGTTCGCGTGACAGAATTTAAAAATAAAGCCCCAAAATTTAGATTTGTAGGCAGGAAAAACGGGGGTTTAAGCATTAGTAATGAGAGAACAGGTGAAGCAGAACTTCATAAGGCTGTAGAAGAAGCAGGCCGTGTATTGAAGCCATATGGGGCAGCAATTATGGACTACAGAAGCTATGCTGATAGCTCAACAATTCCAGGACACTACGTATTGTACTTGGAATTAATATTTGATCATGAGTTTAAGGGACAAATTTCCAATTCCATTCCATGTTCGGTATATGAGGATTGTTGTCTTGCCATTGAAAATTCACTTAGTGCACTCTAA

mRNA sequence

ATGCCGCATATTGAAGAGAATTGGTTGGATGAGAAAGATATGGAAGCATTACAACACATTGAAGATTTCACAAGCAAAGCCGATGAAATTCAGCGACAAATTTTAACTGAAATTCTGTCAACAAATGCTGATGTTGAATACTTGCAACGACATGGCCTTGATGGCTCTACTGATGCTTCAACCTTCAAGAAATCCAGCGGAAGGAATTTTTTTTTGTTCCTTGAAAACCATTGGGTTGTTTTGGCTAATGATATACGAACAGGAAGTATTAACCCCAAAATTACAGACTCATCAGTGAGGGAATCAGTTATGAAGATTGTTGTAAAACCCAATCCTGAAGTTGCTGATTTGATTGAACATGAATGTAGCAAAGGAAGCTGGGAAGGAATTATTAAAAGATTGTGGCCAAACACCAAATACATTAATGCTATTGCGACAGGAAGTATGTCACAATACATTCCATTGCTAAATTACTACACCAATAATCTCCCTATTGTTTCTGACCATTATGGTTCTTCAGAATGTTTCCTTGGCTTAAACTTAGATCCATTGTGTGACCCTTATGAAATCTCCTACACTTTGATACCCACCATGGCGTATTTCGAGTTCTTGCCAATCGATAGGACAAATCCCCATGGAGGAAGAAGCCAAGAATTAGTTGATCTTGTTGATGTCAAGTTAGGACAAGAATATGAGCTAGTCATCACAACTTTTGCAGGCAGGAAAAACGGGGGTTTAAGCATTAGTAATGAGAGAACAGGTGAAGCAGAACTTCATAAGGCTGTAGAAGAAGCAGGCCGTGTATTGAAGCCATATGGGGCAGCAATTATGGACTACAGAAGCTATGCTGATAGCTCAACAATTCCAGGACACTACGTATTGTACTTGGAATTAATATTTGATCATGAGTTTAAGGGACAAATTTCCAATTCCATTCCATGTTCGGTATATGAGGATTGTTGTCTTGCCATTGAAAATTCACTTAGTGCACTCTAA

Coding sequence (CDS)

ATGCCGCATATTGAAGAGAATTGGTTGGATGAGAAAGATATGGAAGCATTACAACACATTGAAGATTTCACAAGCAAAGCCGATGAAATTCAGCGACAAATTTTAACTGAAATTCTGTCAACAAATGCTGATGTTGAATACTTGCAACGACATGGCCTTGATGGCTCTACTGATGCTTCAACCTTCAAGAAATCCAGCGGAAGGAATTTTTTTTTGTTCCTTGAAAACCATTGGGTTGTTTTGGCTAATGATATACGAACAGGAAGTATTAACCCCAAAATTACAGACTCATCAGTGAGGGAATCAGTTATGAAGATTGTTGTAAAACCCAATCCTGAAGTTGCTGATTTGATTGAACATGAATGTAGCAAAGGAAGCTGGGAAGGAATTATTAAAAGATTGTGGCCAAACACCAAATACATTAATGCTATTGCGACAGGAAGTATGTCACAATACATTCCATTGCTAAATTACTACACCAATAATCTCCCTATTGTTTCTGACCATTATGGTTCTTCAGAATGTTTCCTTGGCTTAAACTTAGATCCATTGTGTGACCCTTATGAAATCTCCTACACTTTGATACCCACCATGGCGTATTTCGAGTTCTTGCCAATCGATAGGACAAATCCCCATGGAGGAAGAAGCCAAGAATTAGTTGATCTTGTTGATGTCAAGTTAGGACAAGAATATGAGCTAGTCATCACAACTTTTGCAGGCAGGAAAAACGGGGGTTTAAGCATTAGTAATGAGAGAACAGGTGAAGCAGAACTTCATAAGGCTGTAGAAGAAGCAGGCCGTGTATTGAAGCCATATGGGGCAGCAATTATGGACTACAGAAGCTATGCTGATAGCTCAACAATTCCAGGACACTACGTATTGTACTTGGAATTAATATTTGATCATGAGTTTAAGGGACAAATTTCCAATTCCATTCCATGTTCGGTATATGAGGATTGTTGTCTTGCCATTGAAAATTCACTTAGTGCACTCTAA

Protein sequence

MPHIEENWLDEKDMEALQHIEDFTSKADEIQRQILTEILSTNADVEYLQRHGLDGSTDASTFKKSSGRNFFLFLENHWVVLANDIRTGSINPKITDSSVRESVMKIVVKPNPEVADLIEHECSKGSWEGIIKRLWPNTKYINAIATGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCDPYEISYTLIPTMAYFEFLPIDRTNPHGGRSQELVDLVDVKLGQEYELVITTFAGRKNGGLSISNERTGEAELHKAVEEAGRVLKPYGAAIMDYRSYADSSTIPGHYVLYLELIFDHEFKGQISNSIPCSVYEDCCLAIENSLSAL
Homology
BLAST of Bhi10G000939 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 299.7 bits (766), Expect = 3.0e-81
Identity = 155/296 (52.36%), Postives = 195/296 (65.88%), Query Frame = 0

Query: 73  FLENHWVVLANDIRTGSINPKITDSSVRESVMKIVVKPNPEVADLIEHECSKGSWEGIIK 132
           FLE HW+ L  DIRTG+++  ITD SVRE+V KI +KP+P++AD +E EC K SW+GII 
Sbjct: 242 FLEKHWIELVRDIRTGTLSSLITDPSVREAVAKI-LKPSPKLADFVEFECKKSSWQGIIT 301

Query: 133 RLWPNTKYINAIATGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCDPYEISY 192
           RLWPNTKY++ I TG+MSQYIP L+YY+N LP+V   Y SSEC+ G+NL PLC P E+SY
Sbjct: 302 RLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSY 361

Query: 193 TLIPTMAYFEFLPIDRTN--------PHG---GRSQELVDLVDVKLGQEYELVITTFAG- 252
           TLIP+MAYFEFLP+ R N        P        QELVDLVDVKLGQEYELV+TT+AG 
Sbjct: 362 TLIPSMAYFEFLPVHRNNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGL 421

Query: 253 -------------------------RKNGGLSISNERTGEAELHKAVEEAGRVLKPYGAA 312
                                    RKN  LSI +++T E EL  AV+ A   L P+ A+
Sbjct: 422 CRYRVGDLLRVTGFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDAS 481

Query: 313 IMDYRSYADSSTIPGHYVLYLELIFDHEFKGQISNSIPCSVYEDCCLAIENSLSAL 332
           + +Y SYAD+S+IPGHYVL+ EL  D       +  IP SV+EDCCLA+E S + +
Sbjct: 482 LSEYTSYADTSSIPGHYVLFWELCLDG------NTPIPPSVFEDCCLAVEESFNTV 530


HSP 2 Score: 71.2 bits (173), Expect = 1.7e-12
Identity = 34/55 (61.82%), Postives = 44/55 (80.00%), Query Frame = 0

Query: 9  LDEKDMEALQHIEDFTSKADEIQRQILTEILSTNADVEYLQRHGLDGSTDASTFK 64
          LD+K+ + LQ IE+ TS AD++QRQ+L EIL+ NADVEYL+RH L+G TD  TFK
Sbjct: 17 LDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFK 71

BLAST of Bhi10G000939 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 298.5 bits (763), Expect = 6.6e-81
Identity = 195/526 (37.07%), Postives = 249/526 (47.34%), Query Frame = 0

Query: 4   IEENWLDEKDMEALQHIEDFTSKADEIQRQILTEILSTNADVEYLQRHGLDGSTDASTFK 63
           + +  L EK+   LQ IED T+ AD++QR++L EILS NADVEYL+RHGL+G TD  TFK
Sbjct: 12  VSDESLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFK 71

Query: 64  ------------------------------------KSSGRN------------------ 123
                                                SSG +                  
Sbjct: 72  HIMPVVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRS 131

Query: 124 -----------------------FFL---------------------------------- 183
                                  +FL                                  
Sbjct: 132 LLYSLLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDP 191

Query: 184 --------------------------------------------------FLENHWVVLA 243
                                                             FLE HW  LA
Sbjct: 192 YTNYTSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELA 251

Query: 244 NDIRTGSINPKITDSSVRESVMKIVVKPNPEVADLIEHECSKGSWEGIIKRLWPNTKYIN 303
            DIRTG+++ +ITDSSVRE+V +I +KP+P++AD +E EC K SW+GII RLWPNTKY++
Sbjct: 252 RDIRTGTLSSEITDSSVREAVGEI-LKPDPKLADFVESECRKTSWQGIITRLWPNTKYVD 311

Query: 304 AIATGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCDPYEISYTLIPTMAYFE 332
            I TG+MSQYIP L+YY+N LP+V   Y SSEC+ G+NL PLC P E+SYTLIP MAYFE
Sbjct: 312 VIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFE 371

BLAST of Bhi10G000939 vs. TAIR 10
Match: AT1G59500.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 255.4 bits (651), Expect = 6.4e-68
Identity = 126/286 (44.06%), Postives = 184/286 (64.34%), Query Frame = 0

Query: 73  FLENHWVVLANDIRTGSINPKITDSSVRESVMKIVVKPNPEVADLIEHECSKGSWEGIIK 132
           FL+N+W  L+ DI TG+++ KI D +++  +  I+ KP+ E+A+ +   CS+ +WEGII 
Sbjct: 239 FLQNNWKELSQDISTGTLSSKIFDHAIKTRMSNILNKPDQELAEFLIGVCSQENWEGIIT 298

Query: 133 RLWPNTKYINAIATGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCDPYEISY 192
           ++WPNTKY++ I TG+M++YIP+L YY+  LP+ S  Y SSE + G+NL+P+C P E+SY
Sbjct: 299 KIWPNTKYLDVIVTGAMAEYIPMLEYYSGGLPMASMIYASSESYFGINLNPMCKPSEVSY 358

Query: 193 TLIPTMAYFEFLPIDRTNPHGGRSQELVDLVDVKLGQEYELVITTFAG------------ 252
           T+ P MAYFEFLP +     G  +  LV+L DV++G+EYELVITT+AG            
Sbjct: 359 TIFPNMAYFEFLPHNHDGDGGVEATSLVELADVEVGKEYELVITTYAGLYRYRVGDILRV 418

Query: 253 --------------RKNGGLSISNERTGEAELHKAVEEAGRVLKPYGAAIMDYRSYADSS 312
                         R+N  LSI +++T EA+L KAVE A R+L   G  +++Y SYAD+ 
Sbjct: 419 TGFHNSAPQFKFIRRENVLLSIESDKTDEADLQKAVENASRLLAEQGTRVIEYTSYADTK 478

Query: 313 TIPGHYVLYLELIFDHEFKGQISNSIPC-SVYEDCCLAIENSLSAL 332
           TIPGHYV+Y EL+   +     SN++P   V   CCL +E SL+A+
Sbjct: 479 TIPGHYVIYWELLSRDQ-----SNALPSDEVMAKCCLEMEESLNAV 519


HSP 2 Score: 50.1 bits (118), Expect = 4.1e-06
Identity = 23/53 (43.40%), Postives = 37/53 (69.81%), Query Frame = 0

Query: 11 EKDMEALQHIEDFTSKADEIQRQILTEILSTNADVEYLQRHGLDGSTDASTFK 64
          E +++AL+ IE+ T   D +Q ++L EILS N++ EYL+R  L+G+ D  +FK
Sbjct: 18 ETEVKALKFIEEITRNPDSVQEKVLGEILSRNSNTEYLKRFDLNGAVDRKSFK 70

BLAST of Bhi10G000939 vs. TAIR 10
Match: AT5G13370.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 250.8 bits (639), Expect = 1.6e-66
Identity = 133/310 (42.90%), Postives = 184/310 (59.35%), Query Frame = 0

Query: 55  GSTDASTFKKSSGRNFFLFLENHWVVLANDIRTGSINPKITDSSVRESVMKIVVKPNPEV 114
           G+  AS+F K        FLE+HW  L ++IRTG ++  ITD++    + K +  PNPE+
Sbjct: 216 GAPFASSFLK-----VIKFLEDHWPELCSNIRTGRLSDWITDATCTSGIGKFLTAPNPEL 275

Query: 115 ADLIEHECSKGSWEGIIKRLWPNTKYINAIATGSMSQYIPLLNYYTNNLPIVSDHYGSSE 174
           A LIE ECSK SWE I+KRLWP  K I +I TG+M+QYIPLL +Y+  LP+ S  YGSSE
Sbjct: 276 ASLIEQECSKTSWEAILKRLWPKAKCIESIITGTMAQYIPLLEFYSGGLPLTSSFYGSSE 335

Query: 175 CFLGLNLDPLCDPYEISYTLIPTMAYFEFLPIDRTNPHGGRSQE----LVDLVDVKLGQE 234
           CF+G+N +PLC P ++SYT+IP M YFEFL +++ +   G        +VDLVDVK+G +
Sbjct: 336 CFMGVNFNPLCKPSDVSYTIIPCMGYFEFLEVEKDHQEAGHDPTEKPVVVDLVDVKIGHD 395

Query: 235 YELVITTFA--------------------------GRKNGGLSISNERTGEAELHKAVEE 294
           YE V+TTF+                          GR+   LSI  ++T E +L KAV  
Sbjct: 396 YEPVVTTFSGLYRYRVGDVLRATGFYNNAPHFCFVGRQKVVLSIDMDKTYEDDLLKAVTN 455

Query: 295 AGRVLKPYGAAIMDYRSYADSSTIPGHYVLYLEL---IFDHEFKGQISNSIPCSVYEDCC 332
           A  +L+P+   +MD+ S  DSS+ PGHYV+Y EL   + D +F+          V E+CC
Sbjct: 456 AKLLLEPHDLMLMDFTSRVDSSSFPGHYVIYWELGSKVKDAKFEPN------RDVMEECC 514

BLAST of Bhi10G000939 vs. TAIR 10
Match: AT5G13320.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 250.4 bits (638), Expect = 2.1e-66
Identity = 128/282 (45.39%), Postives = 173/282 (61.35%), Query Frame = 0

Query: 74  LENHWVVLANDIRTGSINPKITDSSVRESVMKIVVKPNPEVADLIEHECSKGSWEGIIKR 133
           L+N W  L ++IR+G ++  +TD   + SV  ++  P PE+AD IE  C++ SW+GI+KR
Sbjct: 229 LKNSWEELCSNIRSGHLSNWVTDLGCQNSVSLVLGGPRPELADTIEEICNQNSWKGIVKR 288

Query: 134 LWPNTKYINAIATGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCDPYEISYT 193
           LWPNTKYI  + TGSM QY+P+LNYY N+LP+VS  YGSSE   G+NLDPLC P ++SYT
Sbjct: 289 LWPNTKYIETVVTGSMGQYVPMLNYYCNDLPLVSTTYGSSETTFGINLDPLCKPEDVSYT 348

Query: 194 LIPTMAYFEFLPIDRTNPHGGRSQELVDLVDVKLGQEYELVITTFAG------------- 253
            +P M+YFEF+P+D     GG   ++VDL DVKLG  YE V+T FAG             
Sbjct: 349 FMPNMSYFEFIPMD-----GGDKNDVVDLEDVKLGCTYEPVVTNFAGLYRMRVGDIVLVT 408

Query: 254 -------------RKNGGLSISNERTGEAELHKAVEEAGRVLKPYGAAIMDYRSYADSST 313
                        R+N  LSI +++T E +L KAV +A  VL+  G  + D+ SYAD+ST
Sbjct: 409 GFYNNAPQFKFVRRENVVLSIDSDKTNEEDLFKAVSQAKLVLESSGLDLKDFTSYADTST 468

Query: 314 IPGHYVLYLEL-IFDHEFKGQISNSIPCSVYEDCCLAIENSL 329
            PGHYV+YLE+   + E K      +       CCL +E SL
Sbjct: 469 FPGHYVVYLEVDTKEGEEKETAQFELDEEALSTCCLVMEESL 505

BLAST of Bhi10G000939 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 4.2e-80
Identity = 155/296 (52.36%), Postives = 195/296 (65.88%), Query Frame = 0

Query: 73  FLENHWVVLANDIRTGSINPKITDSSVRESVMKIVVKPNPEVADLIEHECSKGSWEGIIK 132
           FLE HW+ L  DIRTG+++  ITD SVRE+V KI +KP+P++AD +E EC K SW+GII 
Sbjct: 242 FLEKHWIELVRDIRTGTLSSLITDPSVREAVAKI-LKPSPKLADFVEFECKKSSWQGIIT 301

Query: 133 RLWPNTKYINAIATGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCDPYEISY 192
           RLWPNTKY++ I TG+MSQYIP L+YY+N LP+V   Y SSEC+ G+NL PLC P E+SY
Sbjct: 302 RLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSY 361

Query: 193 TLIPTMAYFEFLPIDRTN--------PHG---GRSQELVDLVDVKLGQEYELVITTFAG- 252
           TLIP+MAYFEFLP+ R N        P        QELVDLVDVKLGQEYELV+TT+AG 
Sbjct: 362 TLIPSMAYFEFLPVHRNNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGL 421

Query: 253 -------------------------RKNGGLSISNERTGEAELHKAVEEAGRVLKPYGAA 312
                                    RKN  LSI +++T E EL  AV+ A   L P+ A+
Sbjct: 422 CRYRVGDLLRVTGFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDAS 481

Query: 313 IMDYRSYADSSTIPGHYVLYLELIFDHEFKGQISNSIPCSVYEDCCLAIENSLSAL 332
           + +Y SYAD+S+IPGHYVL+ EL  D       +  IP SV+EDCCLA+E S + +
Sbjct: 482 LSEYTSYADTSSIPGHYVLFWELCLDG------NTPIPPSVFEDCCLAVEESFNTV 530


HSP 2 Score: 71.2 bits (173), Expect = 2.4e-11
Identity = 34/55 (61.82%), Postives = 44/55 (80.00%), Query Frame = 0

Query: 9  LDEKDMEALQHIEDFTSKADEIQRQILTEILSTNADVEYLQRHGLDGSTDASTFK 64
          LD+K+ + LQ IE+ TS AD++QRQ+L EIL+ NADVEYL+RH L+G TD  TFK
Sbjct: 17 LDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFK 71

BLAST of Bhi10G000939 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 298.5 bits (763), Expect = 9.3e-80
Identity = 195/526 (37.07%), Postives = 249/526 (47.34%), Query Frame = 0

Query: 4   IEENWLDEKDMEALQHIEDFTSKADEIQRQILTEILSTNADVEYLQRHGLDGSTDASTFK 63
           + +  L EK+   LQ IED T+ AD++QR++L EILS NADVEYL+RHGL+G TD  TFK
Sbjct: 12  VSDESLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFK 71

Query: 64  ------------------------------------KSSGRN------------------ 123
                                                SSG +                  
Sbjct: 72  HIMPVVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRS 131

Query: 124 -----------------------FFL---------------------------------- 183
                                  +FL                                  
Sbjct: 132 LLYSLLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDP 191

Query: 184 --------------------------------------------------FLENHWVVLA 243
                                                             FLE HW  LA
Sbjct: 192 YTNYTSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELA 251

Query: 244 NDIRTGSINPKITDSSVRESVMKIVVKPNPEVADLIEHECSKGSWEGIIKRLWPNTKYIN 303
            DIRTG+++ +ITDSSVRE+V +I +KP+P++AD +E EC K SW+GII RLWPNTKY++
Sbjct: 252 RDIRTGTLSSEITDSSVREAVGEI-LKPDPKLADFVESECRKTSWQGIITRLWPNTKYVD 311

Query: 304 AIATGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCDPYEISYTLIPTMAYFE 332
            I TG+MSQYIP L+YY+N LP+V   Y SSEC+ G+NL PLC P E+SYTLIP MAYFE
Sbjct: 312 VIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFE 371

BLAST of Bhi10G000939 vs. ExPASy Swiss-Prot
Match: Q8LQM5 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 4.0e-75
Identity = 138/286 (48.25%), Postives = 194/286 (67.83%), Query Frame = 0

Query: 73  FLENHWVVLANDIRTGSINPKITDSSVRESVMKIVVKPNPEVADLIEHECSKGSWEGIIK 132
           FLE HW  L +DIRTG ++P+ITD  VR++V + V++ +P +AD IE EC++ SWEGII+
Sbjct: 242 FLEKHWARLCHDIRTGELDPEITDRVVRDAVGR-VLRADPALADAIEDECARASWEGIIR 301

Query: 133 RLWPNTKYINAIATGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCDPYEISY 192
           RLWP TKYI+ I TG+MSQYIP L +Y   LP+    Y SSEC+ GLNL+P+C P +++Y
Sbjct: 302 RLWPRTKYIDVIVTGTMSQYIPTLEFYGGGLPLTCTMYASSECYFGLNLNPMCKPSDVAY 361

Query: 193 TLIPTMAYFEFLPIDRTNPHGGRS-QELVDLVDVKLGQEYELVITTFAG----------- 252
           TLIPTM Y+EFLP++  N     S ++LVDLVDVKLG EYELV+TT++G           
Sbjct: 362 TLIPTMCYYEFLPVNCNNATAEASHRDLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLR 421

Query: 253 ---------------RKNGGLSISNERTGEAELHKAVEEAGRVLKPYGAAIMDYRSYADS 312
                          R+N  LS+ +++T E ELH AV  A + L P+GA++++Y SYAD+
Sbjct: 422 VAGFKNKAPMFSFVRRQNVALSVDSDKTDETELHAAVSGAVQHLAPFGASLVEYTSYADA 481

Query: 313 STIPGHYVLYLELIFDHEFKGQISNSIPCSVYEDCCLAIENSLSAL 332
           +TIPGHYVL+ EL          S ++P SV+E+CCL++E +L+++
Sbjct: 482 ATIPGHYVLFWELRAG-------STAVPASVFEECCLSVEEALNSV 519


HSP 2 Score: 49.3 bits (116), Expect = 9.8e-05
Identity = 23/44 (52.27%), Postives = 33/44 (75.00%), Query Frame = 0

Query: 15 EALQHIEDFTSKADEIQRQILTEILSTNADVEYLQRHGLDGSTD 59
          EAL+ IE  T+ A ++QR++L EIL+ NA  EYL+R+G+ GS D
Sbjct: 22 EALEFIEHVTANAGQVQRRVLGEILAQNAPAEYLRRYGIPGSPD 65

BLAST of Bhi10G000939 vs. ExPASy Swiss-Prot
Match: A3BLS0 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=39946 GN=GH3.8 PE=1 SV=2)

HSP 1 Score: 271.6 bits (693), Expect = 1.2e-71
Identity = 133/285 (46.67%), Postives = 185/285 (64.91%), Query Frame = 0

Query: 73  FLENHWVVLANDIRTGSINPKITDSSVRESVMKIVVKPNPEVADLIEHECSKGSWEGIIK 132
           FL+ +W  LA+DI +G + P++TD SVRE+V  I++ P+PE+A LI  ECSKG W GII 
Sbjct: 247 FLQLNWEQLADDIESGELTPRVTDPSVREAVAAILL-PDPELAKLIRAECSKGDWAGIIT 306

Query: 133 RLWPNTKYINAIATGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCDPYEISY 192
           R+WPNTKY++ I TG+M+QYIP L +Y+  LP+    Y SSEC+ GLNL P+CDP E+SY
Sbjct: 307 RVWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSY 366

Query: 193 TLIPTMAYFEFLPIDRTNPHGGRSQELVDLVDVKLGQEYELVITTFAG------------ 252
           T++P M YFEFLP+D T    G + +LVDL  V++G+EYELVITT+AG            
Sbjct: 367 TIMPNMGYFEFLPVDETGAASGDATQLVDLARVEVGREYELVITTYAGLNRYRVGDVLRV 426

Query: 253 --------------RKNGGLSISNERTGEAELHKAVEEAGRVLKPYGAAIMDYRSYADSS 312
                         RKN  LSI +++T EAEL +AVE A  +L+P+GA++++Y S A + 
Sbjct: 427 TGFHNAAPQFRFVRRKNVLLSIESDKTDEAELQRAVERASALLRPHGASVVEYTSQACTK 486

Query: 313 TIPGHYVLYLELIFDHEFKGQISNSIPCSVYEDCCLAIENSLSAL 332
            IPGHYV+Y EL+     KG  +  +       CCL +E +L+ +
Sbjct: 487 RIPGHYVIYWELL----TKGAGATVVDADTLGRCCLEMEEALNTV 526

BLAST of Bhi10G000939 vs. ExPASy Swiss-Prot
Match: Q0D4Z6 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.8 PE=1 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 1.2e-71
Identity = 133/285 (46.67%), Postives = 185/285 (64.91%), Query Frame = 0

Query: 73  FLENHWVVLANDIRTGSINPKITDSSVRESVMKIVVKPNPEVADLIEHECSKGSWEGIIK 132
           FL+ +W  LA+DI +G + P++TD SVRE+V  I++ P+PE+A LI  ECSKG W GII 
Sbjct: 247 FLQLNWEQLADDIESGELTPRVTDPSVREAVAAILL-PDPELAKLIRAECSKGDWAGIIT 306

Query: 133 RLWPNTKYINAIATGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCDPYEISY 192
           R+WPNTKY++ I TG+M+QYIP L +Y+  LP+    Y SSEC+ GLNL P+CDP E+SY
Sbjct: 307 RVWPNTKYLDVIVTGAMAQYIPTLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSY 366

Query: 193 TLIPTMAYFEFLPIDRTNPHGGRSQELVDLVDVKLGQEYELVITTFAG------------ 252
           T++P M YFEFLP+D T    G + +LVDL  V++G+EYELVITT+AG            
Sbjct: 367 TIMPNMGYFEFLPVDETGAASGDATQLVDLARVEVGREYELVITTYAGLNRYRVGDVLRV 426

Query: 253 --------------RKNGGLSISNERTGEAELHKAVEEAGRVLKPYGAAIMDYRSYADSS 312
                         RKN  LSI +++T EAEL +AVE A  +L+P+GA++++Y S A + 
Sbjct: 427 TGFHNAAPQFRFVRRKNVLLSIESDKTDEAELQRAVERASALLRPHGASVVEYTSQACTK 486

Query: 313 TIPGHYVLYLELIFDHEFKGQISNSIPCSVYEDCCLAIENSLSAL 332
            IPGHYV+Y EL+     KG  +  +       CCL +E +L+ +
Sbjct: 487 RIPGHYVIYWELL----TKGAGATVVDADTLGRCCLEMEEALNTV 526

BLAST of Bhi10G000939 vs. NCBI nr
Match: XP_038902067.1 (indole-3-acetic acid-amido synthetase GH3.5-like [Benincasa hispida])

HSP 1 Score: 496.5 bits (1277), Expect = 1.8e-136
Identity = 284/518 (54.83%), Postives = 301/518 (58.11%), Query Frame = 0

Query: 1   MPHIEENWLDEKDMEALQHIEDFTSKADEIQRQILTEILSTNADVEYLQRHGLDGSTDAS 60
           M H+ E+WLDEKD EALQHIED TSKADEIQRQILTEILSTNA+VEYLQ+HGL GSTDAS
Sbjct: 1   MSHVVEHWLDEKDKEALQHIEDVTSKADEIQRQILTEILSTNANVEYLQQHGLHGSTDAS 60

Query: 61  TFK--------------------------------------------------------- 120
           TFK                                                         
Sbjct: 61  TFKKLIPLISYEQLTPYLALIAHDDDSPILCSNPITHFFTSSGTSGGETKLIPMYEEEFV 120

Query: 121 -----------------------KSSGRNF------------------------------ 180
                                  K  G NF                              
Sbjct: 121 RRLSFFNYMMPRMKQLFPNINWQKGKGMNFHFAKPEFKTKAGIIVRSIFTSLHKRSSNLK 180

Query: 181 ---------------------------------------------------FLFLENHWV 240
                                                              F FLENHWV
Sbjct: 181 SKPIGNNTSPDDIILCTDSFQSLYCQLLCGLYQNDVVFRLGALFASTLIHVFKFLENHWV 240

Query: 241 VLANDIRTGSINPKITDSSVRESVMKIVVKPNPEVADLIEHECSKGSWEGIIKRLWPNTK 300
           VLANDI TGSINPKIT+ SVRESVMKIVVKPNPEVADLIEHECSKGSWEGIIKRLWPNTK
Sbjct: 241 VLANDILTGSINPKITNLSVRESVMKIVVKPNPEVADLIEHECSKGSWEGIIKRLWPNTK 300

Query: 301 YINAIATGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCDPYEISYTLIPTMA 332
           YINAI TGSMSQYIPLLN+YTNNLPIVSDHYGSSECFLGLNLDPLCDPYEISYTLIPTMA
Sbjct: 301 YINAIVTGSMSQYIPLLNFYTNNLPIVSDHYGSSECFLGLNLDPLCDPYEISYTLIPTMA 360

BLAST of Bhi10G000939 vs. NCBI nr
Match: XP_008448232.1 (PREDICTED: indole-3-acetic acid-amido synthetase GH3.6-like [Cucumis melo] >KAA0062188.1 indole-3-acetic acid-amido synthetase GH3.6-like [Cucumis melo var. makuwa] >TYK04840.1 indole-3-acetic acid-amido synthetase GH3.6-like [Cucumis melo var. makuwa])

HSP 1 Score: 444.9 bits (1143), Expect = 6.1e-121
Identity = 261/518 (50.39%), Postives = 284/518 (54.83%), Query Frame = 0

Query: 1   MPHIEENWLDEKDMEALQHIEDFTSKADEIQRQILTEILSTNADVEYLQRHGLDGSTDAS 60
           M + EE WLDEKD EALQ+IED T+KADEIQRQIL EILS NA+VEYLQ+HGLD STD+S
Sbjct: 1   MSNFEEKWLDEKDKEALQYIEDITTKADEIQRQILNEILSKNANVEYLQKHGLDASTDSS 60

Query: 61  TFK--------------------------------------------------------- 120
           TFK                                                         
Sbjct: 61  TFKKLIPLVYYQQLRPYIARIADGDDSSILCSNPITEFFKSSGTSGEEHKLIPMYEQEFV 120

Query: 121 -----------------------KSSGRNF------------------------------ 180
                                  K+ G NF                              
Sbjct: 121 RRLSFFSYITPRMKQLFPNINWHKAKGLNFHFAKPEFKTKGGIVVRSIFTNLHKRSSNLR 180

Query: 181 ---------------------------------------------------FLFLENHWV 240
                                                              F FLENHW+
Sbjct: 181 SMPSGNNTSPDDIILCTDSYQSLYCQLLCGLYQNDVVFRVSALFASTLIHVFKFLENHWI 240

Query: 241 VLANDIRTGSINPKITDSSVRESVMKIVVKPNPEVADLIEHECSKGSWEGIIKRLWPNTK 300
            LA DIRT +INPKIT SSVRESVMKIVVKPNPEVADLIE+EC KG WEGII RLWPN K
Sbjct: 241 DLATDIRTRTINPKITSSSVRESVMKIVVKPNPEVADLIENECGKGRWEGIITRLWPNAK 300

Query: 301 YINAIATGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCDPYEISYTLIPTMA 332
           YINA+ TGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLC+P E+SYTLIPTMA
Sbjct: 301 YINAVVTGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCNPDEVSYTLIPTMA 360

BLAST of Bhi10G000939 vs. NCBI nr
Match: XP_004140044.1 (indole-3-acetic acid-amido synthetase GH3.6 [Cucumis sativus] >KGN46710.1 hypothetical protein Csa_021088 [Cucumis sativus])

HSP 1 Score: 428.3 bits (1100), Expect = 5.9e-116
Identity = 256/519 (49.33%), Postives = 284/519 (54.72%), Query Frame = 0

Query: 1   MPHIEENWLDEKDMEALQHIEDFTSKADEIQRQILTEILSTNADVEYLQRHGLDGSTDAS 60
           MP +EE WLDEK+ EALQ+IED T+KADEIQRQIL EILSTNA+VEYLQ+HGL   TD+ 
Sbjct: 1   MPDVEEKWLDEKEKEALQYIEDITTKADEIQRQILNEILSTNANVEYLQQHGLVVPTDSP 60

Query: 61  TFKK-------------------------------------------------------- 120
           TFKK                                                        
Sbjct: 61  TFKKLIPLVCYEQLRPYIARIADGDDSSILCSNPITEFFKSSGTSGGEHKLIPMYEQEFV 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 RRLSFFSYIMPRMKQLFPDINWHKVKGLNFHFAKPEFKTKGGIIVRSIFTNLHKRSSNLE 180

Query: 181 --SSGRN-------------------------------------------FFLFLENHWV 240
              SG N                                            F FLENHWV
Sbjct: 181 SMPSGNNTSPDDIILCTDSYQSLYCQLLCGLYQNEVVFRVSALFASTLIHVFKFLENHWV 240

Query: 241 VLANDIRTGSINPKITDSSVRESVMKIVVKPNPEVADLIEHECSKGSWEGIIKRLWPNTK 300
            LA DIRT ++NPKIT+SSVRES+MKI+VKPNPEVADLIE+EC KG WEGII RLWPN K
Sbjct: 241 DLATDIRTRTVNPKITNSSVRESLMKIIVKPNPEVADLIENECRKGRWEGIITRLWPNAK 300

Query: 301 YINAIATGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCDPYEISYTLIPTMA 332
           YINAI TGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLC+  E+SYTLIPTMA
Sbjct: 301 YINAIVTGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCNHDEVSYTLIPTMA 360

BLAST of Bhi10G000939 vs. NCBI nr
Match: KAA0051296.1 (indole-3-acetic acid-amido synthetase GH3.5-like [Cucumis melo var. makuwa])

HSP 1 Score: 407.1 bits (1045), Expect = 1.4e-109
Identity = 246/517 (47.58%), Postives = 282/517 (54.55%), Query Frame = 0

Query: 3   HIEENW-LDEKDMEALQHIEDFTSKADEIQRQILTEILSTNADVEYLQRHGLDGSTDAST 62
           ++E  W LDEKD EALQ I+D TS ADEIQ  ILTEILSTNA+VEYLQ+HGLDGSTD+ST
Sbjct: 4   NVEHKWLLDEKDYEALQQIQDITSNADEIQSLILTEILSTNANVEYLQQHGLDGSTDSST 63

Query: 63  FKK------------------------------------SSG------------------ 122
           FKK                                    SSG                  
Sbjct: 64  FKKLIPLVSYEQLQPYITRIAEGDDSPILCSNPITGFFVSSGTSGGEPKLVPIYEQEFER 123

Query: 123 --------------------------RNF------------------------------- 182
                                      NF                               
Sbjct: 124 RLSFFNYLMARTKQLFPNINCYNDKAMNFHFAKPDHKTKAGILVSSVFTRLLKTSLNLKS 183

Query: 183 --------------------------------------------------FLFLENHWVV 242
                                                             F FLENHW  
Sbjct: 184 IATGNNAIPDDILLCTDTYQSLYCQLLCGLYQNDPVFQVGSIFASGLLHVFKFLENHWAD 243

Query: 243 LANDIRTGSI-NPKITDSSVRESVMKIVVKPNPEVADLIEHECSKGSWEGIIKRLWPNTK 302
           L +DIRTGSI NPKITD S+RESVMKI+VKPNP++ADLIE ECSKG+W+GI++RLWPNTK
Sbjct: 244 LVSDIRTGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGTWKGIVRRLWPNTK 303

Query: 303 YINAIATGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCDPYEISYTLIPTMA 330
           YI AIATGS+SQYIPLLNYYTNNLPI SDHYGS+EC LGLNLDP+CDP E SYTLIPTMA
Sbjct: 304 YIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECLLGLNLDPICDPNETSYTLIPTMA 363

BLAST of Bhi10G000939 vs. NCBI nr
Match: XP_008441807.1 (PREDICTED: indole-3-acetic acid-amido synthetase GH3.5-like [Cucumis melo] >TYJ96956.1 indole-3-acetic acid-amido synthetase GH3.5-like [Cucumis melo var. makuwa])

HSP 1 Score: 407.1 bits (1045), Expect = 1.4e-109
Identity = 246/517 (47.58%), Postives = 282/517 (54.55%), Query Frame = 0

Query: 3   HIEENW-LDEKDMEALQHIEDFTSKADEIQRQILTEILSTNADVEYLQRHGLDGSTDAST 62
           ++E  W LDEKD EALQ I+D TS ADEIQ  ILTEILSTNA+VEYLQ+HGLDGSTD+ST
Sbjct: 4   NVEHKWLLDEKDYEALQQIQDITSNADEIQSLILTEILSTNANVEYLQQHGLDGSTDSST 63

Query: 63  FKK------------------------------------SSG------------------ 122
           FKK                                    SSG                  
Sbjct: 64  FKKLIPLVSYEQLQPYITRIAEGDDSPILCSNPITGFFVSSGTSGGEPKLVPIYEQEFER 123

Query: 123 --------------------------RNF------------------------------- 182
                                      NF                               
Sbjct: 124 RLSFFNYLMARTKQLFPNINCYNDKAMNFHFAKPDHKTKAGILVSSVFTRLLKTSLNLKS 183

Query: 183 --------------------------------------------------FLFLENHWVV 242
                                                             F FLENHW  
Sbjct: 184 IATGNNAIPDDILLCTDTYQSLYCQLLCGLYRNDLVFQVGSIFASGLLHVFKFLENHWAD 243

Query: 243 LANDIRTGSI-NPKITDSSVRESVMKIVVKPNPEVADLIEHECSKGSWEGIIKRLWPNTK 302
           L +DIRTGSI NPKITD S+RESVMKI+VKPNP++ADLIE ECSKG+W+GI++RLWPNTK
Sbjct: 244 LVSDIRTGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGTWKGIVRRLWPNTK 303

Query: 303 YINAIATGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCDPYEISYTLIPTMA 330
           YI AIATGS+SQYIPLLNYYTNNLPI SDHYGS+EC LGLNLDP+CDP E SYTLIPTMA
Sbjct: 304 YIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECLLGLNLDPICDPNETSYTLIPTMA 363

BLAST of Bhi10G000939 vs. ExPASy TrEMBL
Match: A0A5A7V3L5 (Indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G00190 PE=3 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 3.0e-121
Identity = 261/518 (50.39%), Postives = 284/518 (54.83%), Query Frame = 0

Query: 1   MPHIEENWLDEKDMEALQHIEDFTSKADEIQRQILTEILSTNADVEYLQRHGLDGSTDAS 60
           M + EE WLDEKD EALQ+IED T+KADEIQRQIL EILS NA+VEYLQ+HGLD STD+S
Sbjct: 1   MSNFEEKWLDEKDKEALQYIEDITTKADEIQRQILNEILSKNANVEYLQKHGLDASTDSS 60

Query: 61  TFK--------------------------------------------------------- 120
           TFK                                                         
Sbjct: 61  TFKKLIPLVYYQQLRPYIARIADGDDSSILCSNPITEFFKSSGTSGEEHKLIPMYEQEFV 120

Query: 121 -----------------------KSSGRNF------------------------------ 180
                                  K+ G NF                              
Sbjct: 121 RRLSFFSYITPRMKQLFPNINWHKAKGLNFHFAKPEFKTKGGIVVRSIFTNLHKRSSNLR 180

Query: 181 ---------------------------------------------------FLFLENHWV 240
                                                              F FLENHW+
Sbjct: 181 SMPSGNNTSPDDIILCTDSYQSLYCQLLCGLYQNDVVFRVSALFASTLIHVFKFLENHWI 240

Query: 241 VLANDIRTGSINPKITDSSVRESVMKIVVKPNPEVADLIEHECSKGSWEGIIKRLWPNTK 300
            LA DIRT +INPKIT SSVRESVMKIVVKPNPEVADLIE+EC KG WEGII RLWPN K
Sbjct: 241 DLATDIRTRTINPKITSSSVRESVMKIVVKPNPEVADLIENECGKGRWEGIITRLWPNAK 300

Query: 301 YINAIATGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCDPYEISYTLIPTMA 332
           YINA+ TGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLC+P E+SYTLIPTMA
Sbjct: 301 YINAVVTGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCNPDEVSYTLIPTMA 360

BLAST of Bhi10G000939 vs. ExPASy TrEMBL
Match: A0A1S3BJT8 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo OX=3656 GN=LOC103490488 PE=3 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 3.0e-121
Identity = 261/518 (50.39%), Postives = 284/518 (54.83%), Query Frame = 0

Query: 1   MPHIEENWLDEKDMEALQHIEDFTSKADEIQRQILTEILSTNADVEYLQRHGLDGSTDAS 60
           M + EE WLDEKD EALQ+IED T+KADEIQRQIL EILS NA+VEYLQ+HGLD STD+S
Sbjct: 1   MSNFEEKWLDEKDKEALQYIEDITTKADEIQRQILNEILSKNANVEYLQKHGLDASTDSS 60

Query: 61  TFK--------------------------------------------------------- 120
           TFK                                                         
Sbjct: 61  TFKKLIPLVYYQQLRPYIARIADGDDSSILCSNPITEFFKSSGTSGEEHKLIPMYEQEFV 120

Query: 121 -----------------------KSSGRNF------------------------------ 180
                                  K+ G NF                              
Sbjct: 121 RRLSFFSYITPRMKQLFPNINWHKAKGLNFHFAKPEFKTKGGIVVRSIFTNLHKRSSNLR 180

Query: 181 ---------------------------------------------------FLFLENHWV 240
                                                              F FLENHW+
Sbjct: 181 SMPSGNNTSPDDIILCTDSYQSLYCQLLCGLYQNDVVFRVSALFASTLIHVFKFLENHWI 240

Query: 241 VLANDIRTGSINPKITDSSVRESVMKIVVKPNPEVADLIEHECSKGSWEGIIKRLWPNTK 300
            LA DIRT +INPKIT SSVRESVMKIVVKPNPEVADLIE+EC KG WEGII RLWPN K
Sbjct: 241 DLATDIRTRTINPKITSSSVRESVMKIVVKPNPEVADLIENECGKGRWEGIITRLWPNAK 300

Query: 301 YINAIATGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCDPYEISYTLIPTMA 332
           YINA+ TGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLC+P E+SYTLIPTMA
Sbjct: 301 YINAVVTGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCNPDEVSYTLIPTMA 360

BLAST of Bhi10G000939 vs. ExPASy TrEMBL
Match: A0A0A0KAF9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G125240 PE=3 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 2.9e-116
Identity = 256/519 (49.33%), Postives = 284/519 (54.72%), Query Frame = 0

Query: 1   MPHIEENWLDEKDMEALQHIEDFTSKADEIQRQILTEILSTNADVEYLQRHGLDGSTDAS 60
           MP +EE WLDEK+ EALQ+IED T+KADEIQRQIL EILSTNA+VEYLQ+HGL   TD+ 
Sbjct: 1   MPDVEEKWLDEKEKEALQYIEDITTKADEIQRQILNEILSTNANVEYLQQHGLVVPTDSP 60

Query: 61  TFKK-------------------------------------------------------- 120
           TFKK                                                        
Sbjct: 61  TFKKLIPLVCYEQLRPYIARIADGDDSSILCSNPITEFFKSSGTSGGEHKLIPMYEQEFV 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 RRLSFFSYIMPRMKQLFPDINWHKVKGLNFHFAKPEFKTKGGIIVRSIFTNLHKRSSNLE 180

Query: 181 --SSGRN-------------------------------------------FFLFLENHWV 240
              SG N                                            F FLENHWV
Sbjct: 181 SMPSGNNTSPDDIILCTDSYQSLYCQLLCGLYQNEVVFRVSALFASTLIHVFKFLENHWV 240

Query: 241 VLANDIRTGSINPKITDSSVRESVMKIVVKPNPEVADLIEHECSKGSWEGIIKRLWPNTK 300
            LA DIRT ++NPKIT+SSVRES+MKI+VKPNPEVADLIE+EC KG WEGII RLWPN K
Sbjct: 241 DLATDIRTRTVNPKITNSSVRESLMKIIVKPNPEVADLIENECRKGRWEGIITRLWPNAK 300

Query: 301 YINAIATGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCDPYEISYTLIPTMA 332
           YINAI TGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLC+  E+SYTLIPTMA
Sbjct: 301 YINAIVTGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCNHDEVSYTLIPTMA 360

BLAST of Bhi10G000939 vs. ExPASy TrEMBL
Match: A0A5D3BD93 (Indole-3-acetic acid-amido synthetase GH3.5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold976G00010 PE=3 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 6.8e-110
Identity = 246/517 (47.58%), Postives = 282/517 (54.55%), Query Frame = 0

Query: 3   HIEENW-LDEKDMEALQHIEDFTSKADEIQRQILTEILSTNADVEYLQRHGLDGSTDAST 62
           ++E  W LDEKD EALQ I+D TS ADEIQ  ILTEILSTNA+VEYLQ+HGLDGSTD+ST
Sbjct: 4   NVEHKWLLDEKDYEALQQIQDITSNADEIQSLILTEILSTNANVEYLQQHGLDGSTDSST 63

Query: 63  FKK------------------------------------SSG------------------ 122
           FKK                                    SSG                  
Sbjct: 64  FKKLIPLVSYEQLQPYITRIAEGDDSPILCSNPITGFFVSSGTSGGEPKLVPIYEQEFER 123

Query: 123 --------------------------RNF------------------------------- 182
                                      NF                               
Sbjct: 124 RLSFFNYLMARTKQLFPNINCYNDKAMNFHFAKPDHKTKAGILVSSVFTRLLKTSLNLKS 183

Query: 183 --------------------------------------------------FLFLENHWVV 242
                                                             F FLENHW  
Sbjct: 184 IATGNNAIPDDILLCTDTYQSLYCQLLCGLYRNDLVFQVGSIFASGLLHVFKFLENHWAD 243

Query: 243 LANDIRTGSI-NPKITDSSVRESVMKIVVKPNPEVADLIEHECSKGSWEGIIKRLWPNTK 302
           L +DIRTGSI NPKITD S+RESVMKI+VKPNP++ADLIE ECSKG+W+GI++RLWPNTK
Sbjct: 244 LVSDIRTGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGTWKGIVRRLWPNTK 303

Query: 303 YINAIATGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCDPYEISYTLIPTMA 330
           YI AIATGS+SQYIPLLNYYTNNLPI SDHYGS+EC LGLNLDP+CDP E SYTLIPTMA
Sbjct: 304 YIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECLLGLNLDPICDPNETSYTLIPTMA 363

BLAST of Bhi10G000939 vs. ExPASy TrEMBL
Match: A0A5A7UAR7 (Indole-3-acetic acid-amido synthetase GH3.5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold55G00030 PE=3 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 6.8e-110
Identity = 246/517 (47.58%), Postives = 282/517 (54.55%), Query Frame = 0

Query: 3   HIEENW-LDEKDMEALQHIEDFTSKADEIQRQILTEILSTNADVEYLQRHGLDGSTDAST 62
           ++E  W LDEKD EALQ I+D TS ADEIQ  ILTEILSTNA+VEYLQ+HGLDGSTD+ST
Sbjct: 4   NVEHKWLLDEKDYEALQQIQDITSNADEIQSLILTEILSTNANVEYLQQHGLDGSTDSST 63

Query: 63  FKK------------------------------------SSG------------------ 122
           FKK                                    SSG                  
Sbjct: 64  FKKLIPLVSYEQLQPYITRIAEGDDSPILCSNPITGFFVSSGTSGGEPKLVPIYEQEFER 123

Query: 123 --------------------------RNF------------------------------- 182
                                      NF                               
Sbjct: 124 RLSFFNYLMARTKQLFPNINCYNDKAMNFHFAKPDHKTKAGILVSSVFTRLLKTSLNLKS 183

Query: 183 --------------------------------------------------FLFLENHWVV 242
                                                             F FLENHW  
Sbjct: 184 IATGNNAIPDDILLCTDTYQSLYCQLLCGLYQNDPVFQVGSIFASGLLHVFKFLENHWAD 243

Query: 243 LANDIRTGSI-NPKITDSSVRESVMKIVVKPNPEVADLIEHECSKGSWEGIIKRLWPNTK 302
           L +DIRTGSI NPKITD S+RESVMKI+VKPNP++ADLIE ECSKG+W+GI++RLWPNTK
Sbjct: 244 LVSDIRTGSINNPKITDLSLRESVMKILVKPNPQLADLIETECSKGTWKGIVRRLWPNTK 303

Query: 303 YINAIATGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCDPYEISYTLIPTMA 330
           YI AIATGS+SQYIPLLNYYTNNLPI SDHYGS+EC LGLNLDP+CDP E SYTLIPTMA
Sbjct: 304 YIKAIATGSLSQYIPLLNYYTNNLPIFSDHYGSTECLLGLNLDPICDPNETSYTLIPTMA 363

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT4G27260.13.0e-8152.36Auxin-responsive GH3 family protein [more]
AT5G54510.16.6e-8137.07Auxin-responsive GH3 family protein [more]
AT1G59500.16.4e-6844.06Auxin-responsive GH3 family protein [more]
AT5G13370.11.6e-6642.90Auxin-responsive GH3 family protein [more]
AT5G13320.12.1e-6645.39Auxin-responsive GH3 family protein [more]
Match NameE-valueIdentityDescription
O818294.2e-8052.36Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
Q9LSQ49.3e-8037.07Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=G... [more]
Q8LQM54.0e-7548.25Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japo... [more]
A3BLS01.2e-7146.67Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=399... [more]
Q0D4Z61.2e-7146.67Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japonica OX=3... [more]
Match NameE-valueIdentityDescription
XP_038902067.11.8e-13654.83indole-3-acetic acid-amido synthetase GH3.5-like [Benincasa hispida][more]
XP_008448232.16.1e-12150.39PREDICTED: indole-3-acetic acid-amido synthetase GH3.6-like [Cucumis melo] >KAA0... [more]
XP_004140044.15.9e-11649.33indole-3-acetic acid-amido synthetase GH3.6 [Cucumis sativus] >KGN46710.1 hypoth... [more]
KAA0051296.11.4e-10947.58indole-3-acetic acid-amido synthetase GH3.5-like [Cucumis melo var. makuwa][more]
XP_008441807.11.4e-10947.58PREDICTED: indole-3-acetic acid-amido synthetase GH3.5-like [Cucumis melo] >TYJ9... [more]
Match NameE-valueIdentityDescription
A0A5A7V3L53.0e-12150.39Indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo var. makuwa OX=... [more]
A0A1S3BJT83.0e-12150.39indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A0A0KAF92.9e-11649.33Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G125240 PE=3 SV=1[more]
A0A5D3BD936.8e-11047.58Indole-3-acetic acid-amido synthetase GH3.5-like OS=Cucumis melo var. makuwa OX=... [more]
A0A5A7UAR76.8e-11047.58Indole-3-acetic acid-amido synthetase GH3.5-like OS=Cucumis melo var. makuwa OX=... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004993GH3 familyPFAMPF03321GH3coord: 71..240
e-value: 3.0E-61
score: 207.9
coord: 20..64
e-value: 2.3E-8
score: 33.4
IPR004993GH3 familyPANTHERPTHR31901GH3 DOMAIN-CONTAINING PROTEINcoord: 71..240
IPR004993GH3 familyPANTHERPTHR31901GH3 DOMAIN-CONTAINING PROTEINcoord: 9..64
coord: 241..330
NoneNo IPR availablePANTHERPTHR31901:SF63INDOLE-3-ACETIC ACID-AMIDO SYNTHETASEcoord: 9..64
coord: 241..330
NoneNo IPR availablePANTHERPTHR31901:SF63INDOLE-3-ACETIC ACID-AMIDO SYNTHETASEcoord: 71..240

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi10M000939Bhi10M000939mRNA