Bhi10G000858 (gene) Wax gourd (B227) v1

Overview
NameBhi10G000858
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptionfolate-biopterin transporter 1, chloroplastic
Locationchr10: 23524301 .. 23526854 (+)
RNA-Seq ExpressionBhi10G000858
SyntenyBhi10G000858
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGTTGCCCGTTTTAATTCCAACGACCCCATCCGATTTTGACGATCACACCCTTCTCTGCTCCAATAATGGTAAAGTCATCAATCAATCAATCAATCAATCAATCAATCAATCAAACTTACTGAATTCTCTCTATGTAGCTATCTGAGTAACACCTGAATATGGGTCGGCGTTGAAGTGGGGAAATATATATATGTAAAACCCTTTTTCGGGTTCTCTTTTACTGTTTAATTTTTGAATGTCATTACATTATTAAGGTTGGCCATATGAACTTGGTCCAGGTGTAGAGGAAGGGGACTCGTTTATCTCACGTGTAGATGAAGACTTGTCTAGGTTCAGGCTGAATGGTGTCAAATGCTTTGGTGTAGATTTAACCCCTGATAATGTTGCTGTTGCTATGGTATATTTTGTTCAAGGAGTTTTAGACCTCGCAAAGCTTGCTGTCAGTTTTTATTTAAAAGATGATTTGCATTTAGATCCTGCTGAGGTACGTAGTTTCTTTACATTCATGATTCTTTTCTGTCAACTCTGGTATTTGTTGCCTTAAAAATGCAGAACTCCATTATCTTTGATGTTGCGCTTTCATTGAATTTTATGTAGATTGTAAACTATCATTCTTCTTGTTTTGTAGTCATTTTTAGAATTTTGCTAGTAGTTTTCTGCAGTTCTAAGAAATTAGTTGCTGATAGGTCCTTAATATAATTGTAACAAGTTATGTATGTGTTTGACTAGGCAGCTGTGATTTCTGGTTTTGCTGCATTGCCGTGGCTGATCAAACCTCTCTATGGGTTTATTAGGTACTTATAAGTTCCTGTTTTTTTTTTTTCTAACTATTCAGGACTCAATTAATTTCCTATCTTGTTTATTTTTCATAATAATTGTTTGGTTATTGCAGTGACTCTGTTCCTCTTTTTGGTTACCGAAGAAGGTCACTGTGAAAATTGTTCCTCACCTCTGTTTTCGTTCGATACCTGAAGATGATCTAATGTTGACACATTAACTGGCTTGTTCTCTTGTGGTTATTTTATATTCTAGTTGAAATTTCTTCTTTGTAGCTCAACCTTCATGACTACAGTATTGTTTATGACACAAACTTTCACGTGCCCTACTTAGATAATATTTTCTTCTTTCTTGCCTATATTGGTACTTGTTTCTTATATGTAAGGTCTACGTTGCATCTTTGCTTAAAATTTGATTGTGAACTGGCACTGATTTGATAATATATGCTTGATTACCAATTTTTATTAGAGCAAGGTAAAACAGTTACGCAGTACACGACTTGAACTTGTTTACTGCAGAGTCTTCTTGATCTTTGTTGATTTTCTATGATATATTGCAGAGTTTTCCGTATCAGAAAAATCATTCACTAATCCAATTGAATGTTGATGCGGAGCTGAAGTTTGTAGATCTTTGGGAAGCTCTGTCGCTGTTAAAAGATGGAACGAGAATTGAAACTTCTTACTATATTCCGCTATTGCAAGAATGTATAGATAGGAATTTAGATGCAGAAATGGAAATTATCTATGGAGATATCGTAAAAACTGGGACTCATGAAGACTTATTTGTAATGACTTTTCTTGTTAACGTTTATGTGAAATATGGAGTCATGGAAATTGCTCGCAAAGTGTTTGATAATTTGCCTAGAAGAAATGTCATTGCATGGACAACTCTAGTGACTTGTTATGTTCAGAATACACAACCTTTGGTTGCTTTTTAAGTGTTCATAGAAATGTTGGAAGCTGGAGCTTATCCTACAAACTGTACGCTCGGAATCGTGTTGAATGCTTGTTGTTCCTTGCAATCTATGAATTCGGGAAGCAGGTTCATGCATATATCATCAAATATCATATAGATTTCGACACTAGTATTGGCAACTTAAGTTTCTTCGTCGAGATGCTCTCGGATGGTGTGGAAACAAACGAGTACACTTTAACAAGTGTCTTGAGCGCCTGTTGTGTAATGTTGACATTGGGTTTAGGAGCACAGATTCATTCACTAAGCGTGGAACCTTCATCGAAGGTTAGAACTTTGTAACTATCATTCTCATATGTAGATTTGAACTTATTATATGGTTATCTATGTGTATATTCCTTAAATTCTTGTTGAATATTTAAATTTCAGTTCAATTTTTAACATGTCCCAAAAATTCTAGAATAAATGTGAAAACGCTAGGTTAGCATATAGCTAAAAATCATTACAAGTATGAATTTATGATGAATTTGCTCTACTGACGTTCTTTAACATCCGATTTAAATAGTTAGTATCTAGCTCTATAACAGAATCGGTATTTCCATTACATTAGTAACATCCGATTTAAATAGTTAGTATCTAGCTCTGTAAGCATCATTCTAATATTCGATTTAAATTATTTGATCATTCGATAAGCATCACTACAAGAATTTTGCCTTGAATGTACCATTTATGCACATTCCCAATAAACACGGTTAGTTAAAAATGAATTCTGATAACATTTTCACTTAAACCTTTTTTGTTTGTGTAATTTTTAGGTGTTTGGAAGGGGTGGGGAGGAGATGGATTTCTTGCAACAGTAA

mRNA sequence

ATGTCGTTGCCCGTTTTAATTCCAACGACCCCATCCGATTTTGACGATCACACCCTTCTCTGCTCCAATAATGGTGTAGAGGAAGGGGACTCGTTTATCTCACGTGTAGATGAAGACTTGTCTAGGTTCAGGCTGAATGGTGTCAAATGCTTTGGTGTAGATTTAACCCCTGATAATGTTGCTGTTGCTATGGTATATTTTGTTCAAGGAGTTTTAGACCTCGCAAAGCTTGCTGTCAGTTTTTATTTAAAAGATGATTTGCATTTAGATCCTGCTGAGGCAGCTGTGATTTCTGGTTTTGCTGCATTGCCGTGGCTGATCAAACCTCTCTATGGGTTTATTAGTGACTCTGTTCCTCTTTTTGGTTACCGAAGAAGCTCAACCTTCATGACTACAAGTTTTCCGTATCAGAAAAATCATTCACTAATCCAATTGAATGTTGATGCGGAGCTGAAGTTTGTAGATCTTTGGGAAGCTCTGTCGCTGTTAAAAGATGGAACGAGAATTGAAACTTCTTACTATATTCCGCTATTGCAAGAATGTATAGATAGGAATTTAGATGCAGAAATGGAAATTATCTATGGAGATATCGTAAAAACTGGGACTCATGAAGACTTATTTGTAATGACTTTTCTTGTTAACGTTTATGTGAAATATGGAGTCATGGAAATTGCTCGCAAAGTAAATGTTGGAAGCTGGAGCTTATCCTACAAACTGTACGCTCGGAATCGTGTTGAATGCTTGTTGTTCCTTGCAATCTATGAATTCGGGAAGCAGGTTCATGCATATATCATCAAATATCATATAGATTTCGACACTAGTATTGGCAACTTAAGTTTCTTCGTCGAGATGCTCTCGGATGGTGTGGAAACAAACGAGTACACTTTAACAAGTGTCTTGAGCGCCTGTTGTGTAATGTTGACATTGGGTTTAGGAGCACAGATTCATTCACTAAGCGTGGAACCTTCATCGAAGGTGTTTGGAAGGGGTGGGGAGGAGATGGATTTCTTGCAACAGTAA

Coding sequence (CDS)

ATGTCGTTGCCCGTTTTAATTCCAACGACCCCATCCGATTTTGACGATCACACCCTTCTCTGCTCCAATAATGGTGTAGAGGAAGGGGACTCGTTTATCTCACGTGTAGATGAAGACTTGTCTAGGTTCAGGCTGAATGGTGTCAAATGCTTTGGTGTAGATTTAACCCCTGATAATGTTGCTGTTGCTATGGTATATTTTGTTCAAGGAGTTTTAGACCTCGCAAAGCTTGCTGTCAGTTTTTATTTAAAAGATGATTTGCATTTAGATCCTGCTGAGGCAGCTGTGATTTCTGGTTTTGCTGCATTGCCGTGGCTGATCAAACCTCTCTATGGGTTTATTAGTGACTCTGTTCCTCTTTTTGGTTACCGAAGAAGCTCAACCTTCATGACTACAAGTTTTCCGTATCAGAAAAATCATTCACTAATCCAATTGAATGTTGATGCGGAGCTGAAGTTTGTAGATCTTTGGGAAGCTCTGTCGCTGTTAAAAGATGGAACGAGAATTGAAACTTCTTACTATATTCCGCTATTGCAAGAATGTATAGATAGGAATTTAGATGCAGAAATGGAAATTATCTATGGAGATATCGTAAAAACTGGGACTCATGAAGACTTATTTGTAATGACTTTTCTTGTTAACGTTTATGTGAAATATGGAGTCATGGAAATTGCTCGCAAAGTAAATGTTGGAAGCTGGAGCTTATCCTACAAACTGTACGCTCGGAATCGTGTTGAATGCTTGTTGTTCCTTGCAATCTATGAATTCGGGAAGCAGGTTCATGCATATATCATCAAATATCATATAGATTTCGACACTAGTATTGGCAACTTAAGTTTCTTCGTCGAGATGCTCTCGGATGGTGTGGAAACAAACGAGTACACTTTAACAAGTGTCTTGAGCGCCTGTTGTGTAATGTTGACATTGGGTTTAGGAGCACAGATTCATTCACTAAGCGTGGAACCTTCATCGAAGGTGTTTGGAAGGGGTGGGGAGGAGATGGATTTCTTGCAACAGTAA

Protein sequence

MSLPVLIPTTPSDFDDHTLLCSNNGVEEGDSFISRVDEDLSRFRLNGVKCFGVDLTPDNVAVAMVYFVQGVLDLAKLAVSFYLKDDLHLDPAEAAVISGFAALPWLIKPLYGFISDSVPLFGYRRSSTFMTTSFPYQKNHSLIQLNVDAELKFVDLWEALSLLKDGTRIETSYYIPLLQECIDRNLDAEMEIIYGDIVKTGTHEDLFVMTFLVNVYVKYGVMEIARKVNVGSWSLSYKLYARNRVECLLFLAIYEFGKQVHAYIIKYHIDFDTSIGNLSFFVEMLSDGVETNEYTLTSVLSACCVMLTLGLGAQIHSLSVEPSSKVFGRGGEEMDFLQQ
Homology
BLAST of Bhi10G000858 vs. TAIR 10
Match: AT2G32040.1 (Major facilitator superfamily protein )

HSP 1 Score: 146.7 bits (369), Expect = 3.3e-35
Identity = 86/167 (51.50%), Postives = 107/167 (64.07%), Query Frame = 0

Query: 1   MSLPVLIPTTP--SDFDDHTLLCSNNGVEEGDSFISRVDEDLS---------------RF 60
           MS  V IP  P   D ++  LL S   V EGD+  + V+ D                   
Sbjct: 52  MSSIVSIPELPRRRDSEESLLLDSRISVAEGDTSNTDVEGDRDTTSSIRTQPPPRKGRNL 111

Query: 61  RLNGVKCFGVDLTPDNVAVAMVYFVQGVLDLAKLAVSFYLKDDLHLDPAEAAVISGFAAL 120
             + +K +GV+L+PDNVAVAMVYFVQGVL LA+LAVSFYLKDDLHLDPAE AVI+G ++L
Sbjct: 112 SSSRIKFYGVELSPDNVAVAMVYFVQGVLGLARLAVSFYLKDDLHLDPAETAVITGLSSL 171

Query: 121 PWLIKPLYGFISDSVPLFGYRRSSTFMTTSFPYQKNHSLIQLNVDAE 151
           PWL+KPLYGFISDSVPLFGYRR S  + +      + SL+   VD++
Sbjct: 172 PWLVKPLYGFISDSVPLFGYRRRSYLVLSGLLGAFSWSLMAGFVDSK 218

BLAST of Bhi10G000858 vs. TAIR 10
Match: AT1G79710.1 (Major facilitator superfamily protein )

HSP 1 Score: 63.5 bits (153), Expect = 3.7e-10
Identity = 37/108 (34.26%), Postives = 61/108 (56.48%), Query Frame = 0

Query: 62  VAMVYFV-QGV-LDLAKLAVSFYLKDDLHLDPAEAAVISGFAALPWLIKPLYGFISDSVP 121
           V +VY V QG+   L+K++  +Y KD+  + P++A +  G   +PW+IKPL+G ++D VP
Sbjct: 53  VIIVYGVSQGLGKGLSKVSTQYYFKDEQKIQPSQAQIYVGLIQIPWIIKPLWGLLTDVVP 112

Query: 122 LFGYRRSSTFMTTSFPYQKNHSLIQLNVDAELKFVDLWEALSLLKDGT 168
           + GYRR   F+   F    +  ++ L+ +  L       ALS L  G+
Sbjct: 113 VLGYRRRPYFILAGFLAMISMMVLWLHTNLHLAL-----ALSCLVAGS 155

BLAST of Bhi10G000858 vs. TAIR 10
Match: AT5G54860.1 (Major facilitator superfamily protein )

HSP 1 Score: 62.8 bits (151), Expect = 6.2e-10
Identity = 29/65 (44.62%), Postives = 39/65 (60.00%), Query Frame = 0

Query: 62  VAMVYFVQGVLDLAKLAVSFYLKDDLHLDPAEAAVISGFAALPWLIKPLYGFISDSVPLF 121
           V ++YF QG       AVS+ LKD L L P+ +  +   A  PW IKPLYG ISD +P+ 
Sbjct: 20  VCLIYFTQGFRSFVWTAVSYQLKDRLQLSPSASQFVFSVAFFPWSIKPLYGIISDCIPIG 79

Query: 122 GYRRS 127
           G +R+
Sbjct: 80  GKKRT 84

BLAST of Bhi10G000858 vs. TAIR 10
Match: AT5G25050.1 (Major facilitator superfamily protein )

HSP 1 Score: 58.2 bits (139), Expect = 1.5e-08
Identity = 25/88 (28.41%), Postives = 47/88 (53.41%), Query Frame = 0

Query: 74  LAKLAVSFYLKDDLHLDPAEAAVISGFAALPWLIKPLYGFISDSVPLFGYRRSSTFMTTS 133
           L ++A  +Y+KD   + P+E+  ++    +PW+IKPL+G ++D +P+FG+ R   F+   
Sbjct: 63  LGRVATEYYMKDVQKVQPSESQALTAITKIPWIIKPLWGILTDVLPIFGFHRRPYFILAG 122

Query: 134 FPYQKNHSLIQLNVDAELKFVDLWEALS 162
                +   I L+ +  L     W  +S
Sbjct: 123 VLGVVSLLFISLHSNLHLYLALFWMTIS 150

BLAST of Bhi10G000858 vs. TAIR 10
Match: AT5G04780.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 57.0 bits (136), Expect = 3.4e-08
Identity = 43/152 (28.29%), Postives = 68/152 (44.74%), Query Frame = 0

Query: 177 LLQECIDRNLDAEMEIIYGDIVKTGTHEDLFVMTFLVNVYVKYGVMEIARKVNVG----- 236
           +LQ C       E +  +G I++     D+ ++  L+N Y K G +E+AR+V  G     
Sbjct: 67  ILQLCARNGAVMEAKACHGKIIRIDLEGDVTLLNVLINAYSKCGFVELARQVFDGMLERS 126

Query: 237 --SWSLSYKLYARNRVECLLFLAIYEFGKQVHAYIIKYHIDFDTSIGNLSFFVEMLSDGV 296
             SW+    LY RNR+E                               L  F+EM ++G 
Sbjct: 127 LVSWNTMIGLYTRNRMES----------------------------EALDIFLEMRNEGF 186

Query: 297 ETNEYTLTSVLSACCVMLTLGLGAQIHSLSVE 322
           + +E+T++SVLSAC V        ++H LSV+
Sbjct: 187 KFSEFTISSVLSACGVNCDALECKKLHCLSVK 190

BLAST of Bhi10G000858 vs. ExPASy Swiss-Prot
Match: Q9SKZ5 (Folate-biopterin transporter 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g32040 PE=1 SV=2)

HSP 1 Score: 146.7 bits (369), Expect = 4.6e-34
Identity = 86/167 (51.50%), Postives = 107/167 (64.07%), Query Frame = 0

Query: 1   MSLPVLIPTTP--SDFDDHTLLCSNNGVEEGDSFISRVDEDLS---------------RF 60
           MS  V IP  P   D ++  LL S   V EGD+  + V+ D                   
Sbjct: 52  MSSIVSIPELPRRRDSEESLLLDSRISVAEGDTSNTDVEGDRDTTSSIRTQPPPRKGRNL 111

Query: 61  RLNGVKCFGVDLTPDNVAVAMVYFVQGVLDLAKLAVSFYLKDDLHLDPAEAAVISGFAAL 120
             + +K +GV+L+PDNVAVAMVYFVQGVL LA+LAVSFYLKDDLHLDPAE AVI+G ++L
Sbjct: 112 SSSRIKFYGVELSPDNVAVAMVYFVQGVLGLARLAVSFYLKDDLHLDPAETAVITGLSSL 171

Query: 121 PWLIKPLYGFISDSVPLFGYRRSSTFMTTSFPYQKNHSLIQLNVDAE 151
           PWL+KPLYGFISDSVPLFGYRR S  + +      + SL+   VD++
Sbjct: 172 PWLVKPLYGFISDSVPLFGYRRRSYLVLSGLLGAFSWSLMAGFVDSK 218

BLAST of Bhi10G000858 vs. ExPASy Swiss-Prot
Match: Q55721 (Folate-biopterin transporter OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr0642 PE=1 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 3.3e-16
Identity = 39/68 (57.35%), Postives = 52/68 (76.47%), Query Frame = 0

Query: 60  VAVAMVYFVQGVLDLAKLAVSFYLKDDLHLDPAEAAVISGFAALPWLIKPLYGFISDSVP 119
           +A+  +YFVQGVL L++LAVSF+LKD+L L PA    + G  A PW++KP+ G +SD+VP
Sbjct: 37  LAILSIYFVQGVLGLSRLAVSFFLKDELGLSPAAMGALIGLGAAPWILKPVLGLMSDTVP 96

Query: 120 LFGYRRSS 128
           LFGYRR S
Sbjct: 97  LFGYRRRS 104

BLAST of Bhi10G000858 vs. ExPASy Swiss-Prot
Match: Q9SQN2 (Probable folate-biopterin transporter 3 OS=Arabidopsis thaliana OX=3702 GN=At1g79710 PE=2 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 5.1e-09
Identity = 37/108 (34.26%), Postives = 61/108 (56.48%), Query Frame = 0

Query: 62  VAMVYFV-QGV-LDLAKLAVSFYLKDDLHLDPAEAAVISGFAALPWLIKPLYGFISDSVP 121
           V +VY V QG+   L+K++  +Y KD+  + P++A +  G   +PW+IKPL+G ++D VP
Sbjct: 53  VIIVYGVSQGLGKGLSKVSTQYYFKDEQKIQPSQAQIYVGLIQIPWIIKPLWGLLTDVVP 112

Query: 122 LFGYRRSSTFMTTSFPYQKNHSLIQLNVDAELKFVDLWEALSLLKDGT 168
           + GYRR   F+   F    +  ++ L+ +  L       ALS L  G+
Sbjct: 113 VLGYRRRPYFILAGFLAMISMMVLWLHTNLHLAL-----ALSCLVAGS 155

BLAST of Bhi10G000858 vs. ExPASy Swiss-Prot
Match: Q8RWQ5 (Probable folate-biopterin transporter 4 OS=Arabidopsis thaliana OX=3702 GN=At5g54860 PE=2 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 8.8e-09
Identity = 29/65 (44.62%), Postives = 39/65 (60.00%), Query Frame = 0

Query: 62  VAMVYFVQGVLDLAKLAVSFYLKDDLHLDPAEAAVISGFAALPWLIKPLYGFISDSVPLF 121
           V ++YF QG       AVS+ LKD L L P+ +  +   A  PW IKPLYG ISD +P+ 
Sbjct: 20  VCLIYFTQGFRSFVWTAVSYQLKDRLQLSPSASQFVFSVAFFPWSIKPLYGIISDCIPIG 79

Query: 122 GYRRS 127
           G +R+
Sbjct: 80  GKKRT 84

BLAST of Bhi10G000858 vs. ExPASy Swiss-Prot
Match: Q5FV41 (Probable folate-biopterin transporter 2 OS=Arabidopsis thaliana OX=3702 GN=At5g25050 PE=2 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 2.2e-07
Identity = 25/88 (28.41%), Postives = 47/88 (53.41%), Query Frame = 0

Query: 74  LAKLAVSFYLKDDLHLDPAEAAVISGFAALPWLIKPLYGFISDSVPLFGYRRSSTFMTTS 133
           L ++A  +Y+KD   + P+E+  ++    +PW+IKPL+G ++D +P+FG+ R   F+   
Sbjct: 63  LGRVATEYYMKDVQKVQPSESQALTAITKIPWIIKPLWGILTDVLPIFGFHRRPYFILAG 122

Query: 134 FPYQKNHSLIQLNVDAELKFVDLWEALS 162
                +   I L+ +  L     W  +S
Sbjct: 123 VLGVVSLLFISLHSNLHLYLALFWMTIS 150

BLAST of Bhi10G000858 vs. NCBI nr
Match: XP_038900839.1 (folate-biopterin transporter 1, chloroplastic-like isoform X1 [Benincasa hispida])

HSP 1 Score: 256.1 bits (653), Expect = 4.1e-64
Identity = 126/127 (99.21%), Postives = 126/127 (99.21%), Query Frame = 0

Query: 1   MSLPVLIPTTPSDFDDHTLLCSNNGVEEGDSFISRVDEDLSRFRLNGVKCFGVDLTPDNV 60
           MSLPVLIPTTPSDFDDHTLLCSNNGVEEGDSFISRVDEDLSRFRLNGVKCFGVDLTPDNV
Sbjct: 1   MSLPVLIPTTPSDFDDHTLLCSNNGVEEGDSFISRVDEDLSRFRLNGVKCFGVDLTPDNV 60

Query: 61  AVAMVYFVQGVLDLAKLAVSFYLKDDLHLDPAEAAVISGFAALPWLIKPLYGFISDSVPL 120
           AVAMVYFVQGVLDLAKLAVSFYLKDDLHLDPAEAAVISGFAALPWLIKPLYGFISDSVPL
Sbjct: 61  AVAMVYFVQGVLDLAKLAVSFYLKDDLHLDPAEAAVISGFAALPWLIKPLYGFISDSVPL 120

Query: 121 FGYRRSS 128
           FGYRR S
Sbjct: 121 FGYRRRS 127

BLAST of Bhi10G000858 vs. NCBI nr
Match: XP_038885496.1 (putative pentatricopeptide repeat-containing protein At5g52630 [Benincasa hispida])

HSP 1 Score: 253.8 bits (647), Expect = 2.0e-63
Identity = 154/269 (57.25%), Postives = 162/269 (60.22%), Query Frame = 0

Query: 133 SFPYQKNHSLIQLNVDAELKFVDLWEALSLLKDGTRIETSYYIPLLQECIDRNLDAEMEI 192
           S PYQKNHSLIQLNVDAE KFVDL EALSLLKDGTRIETSYYIPLLQECID NL AE EI
Sbjct: 33  SLPYQKNHSLIQLNVDAESKFVDLREALSLLKDGTRIETSYYIPLLQECIDGNLAAEAEI 92

Query: 193 IYGDIVKTGTHEDLFVMTFLVNVYVKYGVMEIARKV-------NVGSWSLSYKLYARNRV 252
           I+G +VKTGTHEDLFVMTFLVNVY K GVMEIARKV       NV +W+     Y +N  
Sbjct: 93  IHGHVVKTGTHEDLFVMTFLVNVYAKCGVMEIARKVFDNLPRRNVIAWTTLVTGYVQNSQ 152

Query: 253 ECLLF---------------------------LAIYEFGKQVHAYIIKYHIDFDTSIGN- 312
             + F                           L   EFGKQVHAYIIKYHIDFDTSIGN 
Sbjct: 153 PLVAFQVFIEMLEAGAYPTNYTLGIVLNACCSLHSIEFGKQVHAYIIKYHIDFDTSIGNS 212

Query: 313 ---------------------------------------------LSFFVEMLSDGVETN 322
                                                        LSFF EMLSD VE N
Sbjct: 213 LSNFYSKSGSLEFAIRAFKKIKKKNVISWTSVVSSCCDNGQAARSLSFFFEMLSDDVEPN 272

BLAST of Bhi10G000858 vs. NCBI nr
Match: XP_038906678.1 (folate-biopterin transporter 1, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 245.4 bits (625), Expect = 7.3e-61
Identity = 121/127 (95.28%), Postives = 122/127 (96.06%), Query Frame = 0

Query: 1   MSLPVLIPTTPSDFDDHTLLCSNNGVEEGDSFISRVDEDLSRFRLNGVKCFGVDLTPDNV 60
           MSLPVLIP TPSD DDHTLLCSNNGVEEG+S ISRVDEDLS FRLNGVKCFGVDLTPDNV
Sbjct: 1   MSLPVLIPITPSDLDDHTLLCSNNGVEEGESLISRVDEDLSSFRLNGVKCFGVDLTPDNV 60

Query: 61  AVAMVYFVQGVLDLAKLAVSFYLKDDLHLDPAEAAVISGFAALPWLIKPLYGFISDSVPL 120
           AVAMVYFVQGVLDLAKLAVSFYLKDDLHLDPAEAAVISGFAALPWLIKPLYGFISDSVPL
Sbjct: 61  AVAMVYFVQGVLDLAKLAVSFYLKDDLHLDPAEAAVISGFAALPWLIKPLYGFISDSVPL 120

Query: 121 FGYRRSS 128
           FGYRR S
Sbjct: 121 FGYRRRS 127

BLAST of Bhi10G000858 vs. NCBI nr
Match: XP_038900840.1 (folate-biopterin transporter 1, chloroplastic-like isoform X2 [Benincasa hispida])

HSP 1 Score: 232.3 bits (591), Expect = 6.4e-57
Identity = 114/114 (100.00%), Postives = 114/114 (100.00%), Query Frame = 0

Query: 1   MSLPVLIPTTPSDFDDHTLLCSNNGVEEGDSFISRVDEDLSRFRLNGVKCFGVDLTPDNV 60
           MSLPVLIPTTPSDFDDHTLLCSNNGVEEGDSFISRVDEDLSRFRLNGVKCFGVDLTPDNV
Sbjct: 1   MSLPVLIPTTPSDFDDHTLLCSNNGVEEGDSFISRVDEDLSRFRLNGVKCFGVDLTPDNV 60

Query: 61  AVAMVYFVQGVLDLAKLAVSFYLKDDLHLDPAEAAVISGFAALPWLIKPLYGFI 115
           AVAMVYFVQGVLDLAKLAVSFYLKDDLHLDPAEAAVISGFAALPWLIKPLYGFI
Sbjct: 61  AVAMVYFVQGVLDLAKLAVSFYLKDDLHLDPAEAAVISGFAALPWLIKPLYGFI 114

BLAST of Bhi10G000858 vs. NCBI nr
Match: XP_022131569.1 (putative pentatricopeptide repeat-containing protein At5g52630 [Momordica charantia])

HSP 1 Score: 228.8 bits (582), Expect = 7.0e-56
Identity = 143/282 (50.71%), Postives = 158/282 (56.03%), Query Frame = 0

Query: 124 RRSSTFMTT----SFPYQKNHSLIQLNVDAELKFVDLWEALSLLKDGTRIETSYYIPLLQ 183
           R SS+F+      S PYQKN SLI+LNVDAE +FVDL EALSLLK+GTRIETSYYIPLLQ
Sbjct: 23  RHSSSFLVADKSGSVPYQKNQSLIRLNVDAEPRFVDLREALSLLKEGTRIETSYYIPLLQ 82

Query: 184 ECIDRNLDAEMEIIYGDIVKTGTHEDLFVMTFLVNVYVKYGVMEIARKV-------NVGS 243
           ECI  NL AE E+I+G I+KTGTHEDLFV TFLVNVY K GVME ARKV       NV +
Sbjct: 83  ECIVSNLAAEAEVIHGHIIKTGTHEDLFVTTFLVNVYAKCGVMEHARKVFDNLPRRNVVA 142

Query: 244 WSLSYKLYARNR---------------------------VECLLFLAIYEFGKQVHAYII 303
           W+     Y +N                            +     L   E GKQVH YII
Sbjct: 143 WTTLVTGYVQNSQPVAALRVFIEMLQAGAYPTNYTLGIVLNACSSLQSIELGKQVHGYII 202

Query: 304 KYHIDFDTSIGN----------------------------------------------LS 322
           KYHIDFDTSIGN                                              LS
Sbjct: 203 KYHIDFDTSIGNSLSSFYSKFGSLDFAIRAFQKIEEKNVISWTSVVSACGDNGQAAQSLS 262

BLAST of Bhi10G000858 vs. ExPASy TrEMBL
Match: A0A6J1BQL4 (putative pentatricopeptide repeat-containing protein At5g52630 OS=Momordica charantia OX=3673 GN=LOC111004719 PE=3 SV=1)

HSP 1 Score: 228.8 bits (582), Expect = 3.4e-56
Identity = 143/282 (50.71%), Postives = 158/282 (56.03%), Query Frame = 0

Query: 124 RRSSTFMTT----SFPYQKNHSLIQLNVDAELKFVDLWEALSLLKDGTRIETSYYIPLLQ 183
           R SS+F+      S PYQKN SLI+LNVDAE +FVDL EALSLLK+GTRIETSYYIPLLQ
Sbjct: 23  RHSSSFLVADKSGSVPYQKNQSLIRLNVDAEPRFVDLREALSLLKEGTRIETSYYIPLLQ 82

Query: 184 ECIDRNLDAEMEIIYGDIVKTGTHEDLFVMTFLVNVYVKYGVMEIARKV-------NVGS 243
           ECI  NL AE E+I+G I+KTGTHEDLFV TFLVNVY K GVME ARKV       NV +
Sbjct: 83  ECIVSNLAAEAEVIHGHIIKTGTHEDLFVTTFLVNVYAKCGVMEHARKVFDNLPRRNVVA 142

Query: 244 WSLSYKLYARNR---------------------------VECLLFLAIYEFGKQVHAYII 303
           W+     Y +N                            +     L   E GKQVH YII
Sbjct: 143 WTTLVTGYVQNSQPVAALRVFIEMLQAGAYPTNYTLGIVLNACSSLQSIELGKQVHGYII 202

Query: 304 KYHIDFDTSIGN----------------------------------------------LS 322
           KYHIDFDTSIGN                                              LS
Sbjct: 203 KYHIDFDTSIGNSLSSFYSKFGSLDFAIRAFQKIEEKNVISWTSVVSACGDNGQAAQSLS 262

BLAST of Bhi10G000858 vs. ExPASy TrEMBL
Match: A0A5A7TH78 (Folate-biopterin transporter 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold306G00280 PE=3 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 3.8e-55
Identity = 112/127 (88.19%), Postives = 114/127 (89.76%), Query Frame = 0

Query: 1   MSLPVLIPTTPSDFDDHTLLCSNNGVEEGDSFISRVDEDLSRFRLNGVKCFGVDLTPDNV 60
           MS PV IPT PSD DDHTLL SNNGVEEGD  ISRVD +LSRFRLN VKCFGVDLTPDNV
Sbjct: 1   MSQPVSIPTAPSDLDDHTLLASNNGVEEGDPLISRVDGELSRFRLNDVKCFGVDLTPDNV 60

Query: 61  AVAMVYFVQGVLDLAKLAVSFYLKDDLHLDPAEAAVISGFAALPWLIKPLYGFISDSVPL 120
           AVA+VYFVQGVL LAKLAV FYLKDDLHLDPAE AVISGFAALPWLIKPLYGFISDSVPL
Sbjct: 61  AVAIVYFVQGVLGLAKLAVGFYLKDDLHLDPAETAVISGFAALPWLIKPLYGFISDSVPL 120

Query: 121 FGYRRSS 128
           FGYRR S
Sbjct: 121 FGYRRRS 127

BLAST of Bhi10G000858 vs. ExPASy TrEMBL
Match: A0A1S3BHD4 (folate-biopterin transporter 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103489673 PE=3 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 8.4e-55
Identity = 111/127 (87.40%), Postives = 114/127 (89.76%), Query Frame = 0

Query: 1   MSLPVLIPTTPSDFDDHTLLCSNNGVEEGDSFISRVDEDLSRFRLNGVKCFGVDLTPDNV 60
           MS PV IPT PSD DDHTLL SNNGVEEGD  ISRVD +LSRFRLN VKCFGVDLTPDNV
Sbjct: 1   MSQPVSIPTAPSDLDDHTLLASNNGVEEGDPLISRVDGELSRFRLNDVKCFGVDLTPDNV 60

Query: 61  AVAMVYFVQGVLDLAKLAVSFYLKDDLHLDPAEAAVISGFAALPWLIKPLYGFISDSVPL 120
           AVA+VYFVQGVL LAKLAV FYLKDDLHLDPAE AVISGF+ALPWLIKPLYGFISDSVPL
Sbjct: 61  AVAIVYFVQGVLGLAKLAVGFYLKDDLHLDPAETAVISGFSALPWLIKPLYGFISDSVPL 120

Query: 121 FGYRRSS 128
           FGYRR S
Sbjct: 121 FGYRRRS 127

BLAST of Bhi10G000858 vs. ExPASy TrEMBL
Match: A0A6J1KJZ5 (pentatricopeptide repeat-containing protein At4g21065-like OS=Cucurbita maxima OX=3661 GN=LOC111495952 PE=3 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 3.2e-54
Identity = 136/271 (50.18%), Postives = 154/271 (56.83%), Query Frame = 0

Query: 131 TTSFPYQKNHSLIQLNVDAELKFVDLWEALSLLKDGTRIETSYYIPLLQECIDRNLDAEM 190
           + S  YQKN SLIQLNVDAE KFVD+ EALSLLK+GT ++TSYY+PLLQEC+DR L AE 
Sbjct: 33  SASVSYQKNQSLIQLNVDAEPKFVDVREALSLLKEGTTVKTSYYVPLLQECVDRKLAAEA 92

Query: 191 EIIYGDIVKTGTHEDLFVMTFLVNVYVKYGVMEIARKV-------NVGSWSLSYKLYARN 250
           EII+G IVKTGTHEDLFVMT LVNVY K GV+E ARKV       NV +W+     Y +N
Sbjct: 93  EIIHGHIVKTGTHEDLFVMTNLVNVYAKCGVVEKARKVFDNLPRRNVVAWTTLVTGYVQN 152

Query: 251 R---------------------------VECLLFLAIYEFGKQVHAYIIKYHIDFDTSIG 310
                                       +     L   E GKQVHAYIIKYH+DFDTSIG
Sbjct: 153 SQPLPALKVFIEMLQAGAYPTNFTLGIVLNACSSLQSIELGKQVHAYIIKYHLDFDTSIG 212

Query: 311 N----------------------------------------------LSFFVEMLSDGVE 322
           N                                              L FF +MLS+GVE
Sbjct: 213 NSLSSFYSKFGCLELAIRAFQKIKEKNVISWTSVVSACGDNGQAARSLCFFTKMLSEGVE 272

BLAST of Bhi10G000858 vs. ExPASy TrEMBL
Match: A0A6J1GH28 (putative pentatricopeptide repeat-containing protein At5g52630 OS=Cucurbita moschata OX=3662 GN=LOC111454156 PE=3 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 1.6e-53
Identity = 137/270 (50.74%), Postives = 153/270 (56.67%), Query Frame = 0

Query: 133 SFPYQKNHSLIQLNVDAELKFVDLWEALSLLKDGTRIETSYYIPLLQECIDRNLDAEMEI 192
           S P QKN SLIQLNVDAE KFVD  EALSLLK+GT ++TSYY+PLLQECIDR L AE EI
Sbjct: 35  SVPNQKNQSLIQLNVDAEPKFVDFREALSLLKEGTTVKTSYYVPLLQECIDRKLAAEAEI 94

Query: 193 IYGDIVKTGTHEDLFVMTFLVNVYVKYGVMEIARKV-------NVGSWSLSYKLYARNRV 252
           I+G IVKTGTHEDLFVMT LVNVY K GVME ARKV       NV  W+     Y +N  
Sbjct: 95  IHGHIVKTGTHEDLFVMTNLVNVYAKCGVMEHARKVFDNLPRRNVVGWTTLVTGYVQNS- 154

Query: 253 ECLLFLAIY----------------------------EFGKQVHAYIIKYHIDFDTSIGN 312
           + L+ L ++                            E GKQVHAYIIKYH+DFDTSIGN
Sbjct: 155 QPLVALKVFIEMLQAGAYPTNFTLGIVLNACSSLQSMELGKQVHAYIIKYHLDFDTSIGN 214

Query: 313 ----------------------------------------------LSFFVEMLSDGVET 322
                                                         L+FF EMLS+GVE 
Sbjct: 215 SLSSFYSKFGCLELAIRAFQKIKEKNVISWTSVVSACGDNGQAARSLNFFTEMLSEGVEP 274

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT2G32040.13.3e-3551.50Major facilitator superfamily protein [more]
AT1G79710.13.7e-1034.26Major facilitator superfamily protein [more]
AT5G54860.16.2e-1044.62Major facilitator superfamily protein [more]
AT5G25050.11.5e-0828.41Major facilitator superfamily protein [more]
AT5G04780.13.4e-0828.29Pentatricopeptide repeat (PPR) superfamily protein [more]
Match NameE-valueIdentityDescription
Q9SKZ54.6e-3451.50Folate-biopterin transporter 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
Q557213.3e-1657.35Folate-biopterin transporter OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=... [more]
Q9SQN25.1e-0934.26Probable folate-biopterin transporter 3 OS=Arabidopsis thaliana OX=3702 GN=At1g7... [more]
Q8RWQ58.8e-0944.62Probable folate-biopterin transporter 4 OS=Arabidopsis thaliana OX=3702 GN=At5g5... [more]
Q5FV412.2e-0728.41Probable folate-biopterin transporter 2 OS=Arabidopsis thaliana OX=3702 GN=At5g2... [more]
Match NameE-valueIdentityDescription
XP_038900839.14.1e-6499.21folate-biopterin transporter 1, chloroplastic-like isoform X1 [Benincasa hispida... [more]
XP_038885496.12.0e-6357.25putative pentatricopeptide repeat-containing protein At5g52630 [Benincasa hispid... [more]
XP_038906678.17.3e-6195.28folate-biopterin transporter 1, chloroplastic isoform X1 [Benincasa hispida][more]
XP_038900840.16.4e-57100.00folate-biopterin transporter 1, chloroplastic-like isoform X2 [Benincasa hispida... [more]
XP_022131569.17.0e-5650.71putative pentatricopeptide repeat-containing protein At5g52630 [Momordica charan... [more]
Match NameE-valueIdentityDescription
A0A6J1BQL43.4e-5650.71putative pentatricopeptide repeat-containing protein At5g52630 OS=Momordica char... [more]
A0A5A7TH783.8e-5588.19Folate-biopterin transporter 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3BHD48.4e-5587.40folate-biopterin transporter 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A6J1KJZ53.2e-5450.18pentatricopeptide repeat-containing protein At4g21065-like OS=Cucurbita maxima O... [more]
A0A6J1GH281.6e-5350.74putative pentatricopeptide repeat-containing protein At5g52630 OS=Cucurbita mosc... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR039309Biopterin transporter familyPFAMPF03092BT1coord: 69..129
e-value: 2.2E-16
score: 59.2
IPR039309Biopterin transporter familyPANTHERPTHR31585FOLATE-BIOPTERIN TRANSPORTER 1, CHLOROPLASTICcoord: 44..132
NoneNo IPR availablePANTHERPTHR31585:SF23FOLATE-BIOPTERIN TRANSPORTER 1 CHLOROPLASTICcoord: 44..132

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi10M000858Bhi10M000858mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane