Bhi10G000754 (gene) Wax gourd (B227) v1

Overview
NameBhi10G000754
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionRING-type domain-containing protein
Locationchr10: 20027172 .. 20028543 (-)
RNA-Seq ExpressionBhi10G000754
SyntenyBhi10G000754
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGATTGCCCAGATCTCTGTTTCTTTTGCGCCTTTTCCTATAAATACAACGCTCAGATTAAATGTCTTCCGAATTACATATATTTTTTTTTGTATAAATATAAATTTCACTTCAAAGAAGAAGTAAACAGAGAGATCGATTTCTTCATTATTATGCTATGGGGTACGGATCGCCATTAACAGCCTTCAATGCTAATGCTACGGCGGTGGAGACGGCGGCTCTGTTTCCGCCGATGGATTCATTCCCAGCGGCGGTGTTGCCAGCGTCGACGGGCGTTGTAATGAAATCCGATAGCGGAATAACTTACAATCTTCCGATTCCGAGAAAGCGCGGCAGAGAACATCATAATTCCAACCGATTCGTTTCATATCCTTCTTCGCATGTTCATTCTCAGAAGAATTGTGGATGTAATTTATATTTTCTCGGTGAAGATATCTCGATTCAAATTCAACAGCAGCAAATCGATGTAGATCGATTGATATCGCAACATGTACGTACGGTACTTGATTAAACTCTGTTAATTTCAAAATTATTCTGTATGTGGATTGAATTTTTTAATTTAGATTTATTTGAAATTAATTAAGCAGATGGAGAGAGTAAAGATGGAATTAGAAGAGAAACAGAAGAGAGAAGCGAGGAGGATTTTGGAGGTAATAGAAATAGGTATGATGAGAATATTGAGAAGCAAAGAGGAAGAAATCGAGAAGATAGGGAAATTAAATTGGGAATTAGAAGAACGAGTAAATTGTTTAAGTATGGAGAATCAATTATGGCGAGATGTGGCTGAAACGAACGAAGCTACGGCGAATGCGTTACGAAGTAATTTAGAACAGGTGCTTTTACAGGTGAATACGGTGGCGGAGGAGGAGGAGGATGCAGAGTCGTGTTGTGGAAGTAATGACGACGGCGGTTGCGGCGGTGGCGGTGGAGTGAGGAAGAAGAGGAAGGAAGGAGATGAAGAAGAAGAGAGAAGGTGTAAGAAATGTTGGAAAGAAGAATCGTGTGTGTTGTTATTGCCATGCAGACATTTGTGTTTGTGTACTGTTTGTGCGTCATCTTTACATACTTGTCCTATTTGTAACTCTACTAATAATGCTAGTGTTCGTGTAATTATGCCTTAGCAATTACTTCATATTTTACATTTCTCTTTTATTTCTTTTTTTCTTTTGCCTCTTTTTCAAATTCTTCTCCATCTTTCTAAATGCAACTCTTCGTGGGACCTATATAAATTTAATTAACATGAAATGTGACTTATATTTGTAGATAAAATTTGTCTTTTTTTTTTTATTTGAAAACAATCCAAAAATAATAATAATAAATTAAAATTTTAAAAATCTTAAAAAGAATTTCTCACATGAGAAACTATAGCCGA

mRNA sequence

GAGATTGCCCAGATCTCTGTTTCTTTTGCGCCTTTTCCTATAAATACAACGCTCAGATTAAATGTCTTCCGAATTACATATATTTTTTTTTGTATAAATATAAATTTCACTTCAAAGAAGAAGTAAACAGAGAGATCGATTTCTTCATTATTATGCTATGGGGTACGGATCGCCATTAACAGCCTTCAATGCTAATGCTACGGCGGTGGAGACGGCGGCTCTGTTTCCGCCGATGGATTCATTCCCAGCGGCGGTGTTGCCAGCGTCGACGGGCGTTGTAATGAAATCCGATAGCGGAATAACTTACAATCTTCCGATTCCGAGAAAGCGCGGCAGAGAACATCATAATTCCAACCGATTCGTTTCATATCCTTCTTCGCATGTTCATTCTCAGAAGAATTGTGGATGTAATTTATATTTTCTCGGTGAAGATATCTCGATTCAAATTCAACAGCAGCAAATCGATGTAGATCGATTGATATCGCAACATATGGAGAGAGTAAAGATGGAATTAGAAGAGAAACAGAAGAGAGAAGCGAGGAGGATTTTGGAGGTAATAGAAATAGGTATGATGAGAATATTGAGAAGCAAAGAGGAAGAAATCGAGAAGATAGGGAAATTAAATTGGGAATTAGAAGAACGAGTAAATTGTTTAAGTATGGAGAATCAATTATGGCGAGATGTGGCTGAAACGAACGAAGCTACGGCGAATGCGTTACGAAGTAATTTAGAACAGGTGCTTTTACAGGTGAATACGGTGGCGGAGGAGGAGGAGGATGCAGAGTCGTGTTGTGGAAGTAATGACGACGGCGGTTGCGGCGGTGGCGGTGGAGTGAGGAAGAAGAGGAAGGAAGGAGATGAAGAAGAAGAGAGAAGGTGTAAGAAATGTTGGAAAGAAGAATCGTGTGTGTTGTTATTGCCATGCAGACATTTGTGTTTGTGTACTGTTTGTGCGTCATCTTTACATACTTGTCCTATTTGTAACTCTACTAATAATGCTAGTGTTCGTGTAATTATGCCTTAGCAATTACTTCATATTTTACATTTCTCTTTTATTTCTTTTTTTCTTTTGCCTCTTTTTCAAATTCTTCTCCATCTTTCTAAATGCAACTCTTCGTGGGACCTATATAAATTTAATTAACATGAAATGTGACTTATATTTGTAGATAAAATTTGTCTTTTTTTTTTTATTTGAAAACAATCCAAAAATAATAATAATAAATTAAAATTTTAAAAATCTTAAAAAGAATTTCTCACATGAGAAACTATAGCCGA

Coding sequence (CDS)

ATGGGGTACGGATCGCCATTAACAGCCTTCAATGCTAATGCTACGGCGGTGGAGACGGCGGCTCTGTTTCCGCCGATGGATTCATTCCCAGCGGCGGTGTTGCCAGCGTCGACGGGCGTTGTAATGAAATCCGATAGCGGAATAACTTACAATCTTCCGATTCCGAGAAAGCGCGGCAGAGAACATCATAATTCCAACCGATTCGTTTCATATCCTTCTTCGCATGTTCATTCTCAGAAGAATTGTGGATGTAATTTATATTTTCTCGGTGAAGATATCTCGATTCAAATTCAACAGCAGCAAATCGATGTAGATCGATTGATATCGCAACATATGGAGAGAGTAAAGATGGAATTAGAAGAGAAACAGAAGAGAGAAGCGAGGAGGATTTTGGAGGTAATAGAAATAGGTATGATGAGAATATTGAGAAGCAAAGAGGAAGAAATCGAGAAGATAGGGAAATTAAATTGGGAATTAGAAGAACGAGTAAATTGTTTAAGTATGGAGAATCAATTATGGCGAGATGTGGCTGAAACGAACGAAGCTACGGCGAATGCGTTACGAAGTAATTTAGAACAGGTGCTTTTACAGGTGAATACGGTGGCGGAGGAGGAGGAGGATGCAGAGTCGTGTTGTGGAAGTAATGACGACGGCGGTTGCGGCGGTGGCGGTGGAGTGAGGAAGAAGAGGAAGGAAGGAGATGAAGAAGAAGAGAGAAGGTGTAAGAAATGTTGGAAAGAAGAATCGTGTGTGTTGTTATTGCCATGCAGACATTTGTGTTTGTGTACTGTTTGTGCGTCATCTTTACATACTTGTCCTATTTGTAACTCTACTAATAATGCTAGTGTTCGTGTAATTATGCCTTAG

Protein sequence

MGYGSPLTAFNANATAVETAALFPPMDSFPAAVLPASTGVVMKSDSGITYNLPIPRKRGREHHNSNRFVSYPSSHVHSQKNCGCNLYFLGEDISIQIQQQQIDVDRLISQHMERVKMELEEKQKREARRILEVIEIGMMRILRSKEEEIEKIGKLNWELEERVNCLSMENQLWRDVAETNEATANALRSNLEQVLLQVNTVAEEEEDAESCCGSNDDGGCGGGGGVRKKRKEGDEEEERRCKKCWKEESCVLLLPCRHLCLCTVCASSLHTCPICNSTNNASVRVIMP
Homology
BLAST of Bhi10G000754 vs. TAIR 10
Match: AT1G79110.2 (zinc ion binding )

HSP 1 Score: 220.7 bits (561), Expect = 1.5e-57
Identity = 149/320 (46.56%), Postives = 191/320 (59.69%), Query Frame = 0

Query: 2   GYGSPLTAFNANATAVETAALFPPMDSFPAA-VLPASTGVVMKSDSGITYN-LPIP-RKR 61
           GYG        N T+  T A  P +  +  +  +P + G      + +     P+P RKR
Sbjct: 42  GYGVVSPFSVPNHTSTTTTATPPLLHVYGGSDTIPTTAGYYADGATNLDCEFFPLPTRKR 101

Query: 62  GREHHNSNRFVSYPSSHVHSQKNCGC----------NLY-FLGEDISI--QIQQQQIDVD 121
            R+   SN    Y    + + ++  C           L+ FLG+DI I   + QQQ ++D
Sbjct: 102 SRDSSRSN----YHHLLLQNPRSSSCVNAATTTTTTTLFSFLGQDIDISSHMNQQQHEID 161

Query: 122 RLISQHMERVKMELEEKQKREARRILEVIEIGMMRILRSKEEEIEKIGKLNWELEERVNC 181
           R +S HMERVK E+EEK+KR+AR I+E IE G+++ LR KEEE E+IGK+N  LEERV  
Sbjct: 162 RFVSLHMERVKYEIEEKRKRQARTIMEAIEQGLVKRLRVKEEERERIGKVNHALEERVKS 221

Query: 182 LSMENQLWRDVAETNEATANALRSNLEQVLLQVNTVA---------EEEEDAESCCGSND 241
           LS+ENQ+WRD+A+TNEATAN LR+NLE VL QV  V+          EE+DAESCCGS+ 
Sbjct: 222 LSIENQIWRDLAQTNEATANHLRTNLEHVLAQVKDVSRGAGLEKNMNEEDDAESCCGSS- 281

Query: 242 DGGCGGGGGVRKKRKEGDEEE---------ERRCKKCWKEESCVLLLPCRHLCLCTVCAS 288
              CGGGG    +R+ G E E          R C+ C +EESCVLLLPCRHLCLC VC S
Sbjct: 282 ---CGGGGEETVRRRVGLEREAQDKAERRRRRMCRNCGEEESCVLLLPCRHLCLCGVCGS 341

BLAST of Bhi10G000754 vs. TAIR 10
Match: AT1G79110.1 (zinc ion binding )

HSP 1 Score: 215.3 bits (547), Expect = 6.4e-56
Identity = 149/323 (46.13%), Postives = 191/323 (59.13%), Query Frame = 0

Query: 2   GYGSPLTAFNANATAVETAALFPPMDSFPAA-VLPASTGVVMKSDSGITYN-LPIP-RKR 61
           GYG        N T+  T A  P +  +  +  +P + G      + +     P+P RKR
Sbjct: 42  GYGVVSPFSVPNHTSTTTTATPPLLHVYGGSDTIPTTAGYYADGATNLDCEFFPLPTRKR 101

Query: 62  GREHHNSNRFVSYPSSHVHSQKNCGC----------NLY-FLGEDISI--QIQQQQIDVD 121
            R+   SN    Y    + + ++  C           L+ FLG+DI I   + QQQ ++D
Sbjct: 102 SRDSSRSN----YHHLLLQNPRSSSCVNAATTTTTTTLFSFLGQDIDISSHMNQQQHEID 161

Query: 122 RLISQH---MERVKMELEEKQKREARRILEVIEIGMMRILRSKEEEIEKIGKLNWELEER 181
           R +S H   MERVK E+EEK+KR+AR I+E IE G+++ LR KEEE E+IGK+N  LEER
Sbjct: 162 RFVSLHLYQMERVKYEIEEKRKRQARTIMEAIEQGLVKRLRVKEEERERIGKVNHALEER 221

Query: 182 VNCLSMENQLWRDVAETNEATANALRSNLEQVLLQVNTVA---------EEEEDAESCCG 241
           V  LS+ENQ+WRD+A+TNEATAN LR+NLE VL QV  V+          EE+DAESCCG
Sbjct: 222 VKSLSIENQIWRDLAQTNEATANHLRTNLEHVLAQVKDVSRGAGLEKNMNEEDDAESCCG 281

Query: 242 SNDDGGCGGGGGVRKKRKEGDEEE---------ERRCKKCWKEESCVLLLPCRHLCLCTV 288
           S+    CGGGG    +R+ G E E          R C+ C +EESCVLLLPCRHLCLC V
Sbjct: 282 SS----CGGGGEETVRRRVGLEREAQDKAERRRRRMCRNCGEEESCVLLLPCRHLCLCGV 341

BLAST of Bhi10G000754 vs. TAIR 10
Match: AT3G12920.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 212.6 bits (540), Expect = 4.1e-55
Identity = 125/272 (45.96%), Postives = 174/272 (63.97%), Query Frame = 0

Query: 28  SFPAAVLPASTGVVMKSDSGITYNLPI-PRKRGREHHNSNRFVSYPSSHVHSQKNCGCNL 87
           S    +    + +   SD+ + +  P+  RKR RE       V  PS+++  QKN    L
Sbjct: 68  SMKPTIQSVDSSLTFNSDNNVDFLRPVSSRKRSRE----ESVVLNPSAYMQIQKNPTDPL 127

Query: 88  YFLGEDISIQIQQQQIDVDRLISQHMERVKMELEEKQKREARRILEVIEIGMMRILRSKE 147
            FLG+D+S  +QQ   D+DRLIS H+ER++ME+EEK+K + RRI+E +E G+M+ LR+K+
Sbjct: 128 MFLGQDLSSNVQQHHFDIDRLISNHVERMRMEIEEKRKTQGRRIVEAVEQGLMKTLRAKD 187

Query: 148 EEIEKIGKLNWELEERVNCLSMENQLWRDVAETNEATANALRSNLEQVLLQVNTVAEEE- 207
           +EI  IGKLN  LEE+V  L +ENQ+WRDVA++NEAT NALRSNL+QVL  V     EE 
Sbjct: 188 DEINHIGKLNLFLEEKVKSLCVENQIWRDVAQSNEATVNALRSNLQQVLAAVERNRWEEP 247

Query: 208 ----EDAESCCGSNDDGGCGGGGGVRKKRKEGDEEEERR--------CKKCWKEESCVLL 267
               +DA+SCCGSND+    G     + +  G+ ++ ++        C+ C K E+ VLL
Sbjct: 248 PTVADDAQSCCGSNDE----GDSEEERWKLAGEAQDTKKMCRVGMSMCRSCGKGEASVLL 307

Query: 268 LPCRHLCLCTVCASSLHTCPICNSTNNASVRV 286
           LPCRH+CLC+VC SSL+TCPIC S   AS+ V
Sbjct: 308 LPCRHMCLCSVCGSSLNTCPICKSPKTASLHV 331

BLAST of Bhi10G000754 vs. TAIR 10
Match: AT4G19700.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 165.6 bits (418), Expect = 5.8e-41
Identity = 108/257 (42.02%), Postives = 155/257 (60.31%), Query Frame = 0

Query: 43  KSDSGITYNLPIP-----RKRGREHHNSNRFVSYPSSHVHSQKNCGCNLYFLGEDISIQI 102
           K++SG++YN  +P     R+R  +  +SN  V   S    S      N+     ++  QI
Sbjct: 61  KAESGLSYNFTVPPLSTKRQRDFQFSDSNAPVKRRSVAFDSSSPSLINV-----ELVSQI 120

Query: 103 Q-QQQIDVDRLISQHMERVKMELEEKQKREARRILEVIEIGMMRILRSKEEEIEKIGKLN 162
           Q QQQ ++DR ++Q  E++++E+E +Q+ + R +   ++  + + L+ K++EI +I  LN
Sbjct: 121 QNQQQSEIDRFVAQQTEKLRIEIEARQQTQTRMLASAVQNVIAKKLKEKDDEIVRIRNLN 180

Query: 163 WELEERVNCLSMENQLWRDVAETNEATANALRSNLEQVLLQVNTV----AEEEEDAESCC 222
           W L+ERV  L +ENQ+WRD+A+TNEA AN LR+NL+QVL Q+ T     A  E+DAES C
Sbjct: 181 WVLQERVKSLYVENQIWRDIAQTNEANANTLRTNLDQVLAQLETFPTASAVVEDDAESSC 240

Query: 223 GSNDDGGCGGGGGVRKKRKEGDEEEERRCKKCWKEESCVLLLPCRHLCLCTVCASS--LH 282
           GS     CG GGG       G       CK+C + E+ VL+LPCRHLCLCTVC  S  L 
Sbjct: 241 GS----CCGDGGGEAVTAVGGG------CKRCGEREASVLVLPCRHLCLCTVCGGSALLR 300

Query: 283 TCPICNSTNNASVRVIM 288
           TCP+C+   NASV V M
Sbjct: 301 TCPVCDMVMNASVHVNM 302

BLAST of Bhi10G000754 vs. TAIR 10
Match: AT5G45100.1 (SBP (S-ribonuclease binding protein) family protein )

HSP 1 Score: 162.2 bits (409), Expect = 6.4e-40
Identity = 97/201 (48.26%), Postives = 132/201 (65.67%), Query Frame = 0

Query: 96  QIQQQQIDVDRLISQHMERVKMELEEKQKREARRILEVIEIGMMRILRSKEEEIEKIGKL 155
           QIQQQ  ++DR ++Q  E +++ELE +Q+ + R +   ++  +++ L++K+EEI ++GKL
Sbjct: 105 QIQQQNSEIDRFVAQQTETLRIELEARQRTQTRMLASAVQNAILKKLKAKDEEIIRMGKL 164

Query: 156 NWELEERVNCLSMENQLWRDVAETNEATANALRSNLEQVLLQVN-------TVAEEEEDA 215
           NW L+ERV  L +ENQ+WRD+A+TNEATAN LRSNLEQVL QV+        + EE +DA
Sbjct: 165 NWVLQERVKNLYVENQIWRDLAQTNEATANNLRSNLEQVLAQVDDLDAFRRPLVEEADDA 224

Query: 216 ESCCGSNDDGGCGGGGGVRKKRKEGDEEEERRCKKCWKEESCVLLLPCRHLCLCTVCASS 275
           ES CGS D      GG V      G       CK+C +  + VL+LPCRHLCLCTVC SS
Sbjct: 225 ESSCGSCD------GGDVTAVVNGG-------CKRCGELTASVLVLPCRHLCLCTVCGSS 284

Query: 276 --LHTCPICNSTNNASVRVIM 288
             L TCP+C+    ASV V M
Sbjct: 285 ALLRTCPVCDMVMTASVHVNM 292

BLAST of Bhi10G000754 vs. ExPASy Swiss-Prot
Match: F4IDI6 (Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=3702 GN=BRG2 PE=1 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 9.0e-55
Identity = 149/323 (46.13%), Postives = 191/323 (59.13%), Query Frame = 0

Query: 2   GYGSPLTAFNANATAVETAALFPPMDSFPAA-VLPASTGVVMKSDSGITYN-LPIP-RKR 61
           GYG        N T+  T A  P +  +  +  +P + G      + +     P+P RKR
Sbjct: 42  GYGVVSPFSVPNHTSTTTTATPPLLHVYGGSDTIPTTAGYYADGATNLDCEFFPLPTRKR 101

Query: 62  GREHHNSNRFVSYPSSHVHSQKNCGC----------NLY-FLGEDISI--QIQQQQIDVD 121
            R+   SN    Y    + + ++  C           L+ FLG+DI I   + QQQ ++D
Sbjct: 102 SRDSSRSN----YHHLLLQNPRSSSCVNAATTTTTTTLFSFLGQDIDISSHMNQQQHEID 161

Query: 122 RLISQH---MERVKMELEEKQKREARRILEVIEIGMMRILRSKEEEIEKIGKLNWELEER 181
           R +S H   MERVK E+EEK+KR+AR I+E IE G+++ LR KEEE E+IGK+N  LEER
Sbjct: 162 RFVSLHLYQMERVKYEIEEKRKRQARTIMEAIEQGLVKRLRVKEEERERIGKVNHALEER 221

Query: 182 VNCLSMENQLWRDVAETNEATANALRSNLEQVLLQVNTVA---------EEEEDAESCCG 241
           V  LS+ENQ+WRD+A+TNEATAN LR+NLE VL QV  V+          EE+DAESCCG
Sbjct: 222 VKSLSIENQIWRDLAQTNEATANHLRTNLEHVLAQVKDVSRGAGLEKNMNEEDDAESCCG 281

Query: 242 SNDDGGCGGGGGVRKKRKEGDEEE---------ERRCKKCWKEESCVLLLPCRHLCLCTV 288
           S+    CGGGG    +R+ G E E          R C+ C +EESCVLLLPCRHLCLC V
Sbjct: 282 SS----CGGGGEETVRRRVGLEREAQDKAERRRRRMCRNCGEEESCVLLLPCRHLCLCGV 341

BLAST of Bhi10G000754 vs. ExPASy Swiss-Prot
Match: Q9LDD1 (Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=3702 GN=BRG3 PE=1 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 5.8e-54
Identity = 125/272 (45.96%), Postives = 174/272 (63.97%), Query Frame = 0

Query: 28  SFPAAVLPASTGVVMKSDSGITYNLPI-PRKRGREHHNSNRFVSYPSSHVHSQKNCGCNL 87
           S    +    + +   SD+ + +  P+  RKR RE       V  PS+++  QKN    L
Sbjct: 68  SMKPTIQSVDSSLTFNSDNNVDFLRPVSSRKRSRE----ESVVLNPSAYMQIQKNPTDPL 127

Query: 88  YFLGEDISIQIQQQQIDVDRLISQHMERVKMELEEKQKREARRILEVIEIGMMRILRSKE 147
            FLG+D+S  +QQ   D+DRLIS H+ER++ME+EEK+K + RRI+E +E G+M+ LR+K+
Sbjct: 128 MFLGQDLSSNVQQHHFDIDRLISNHVERMRMEIEEKRKTQGRRIVEAVEQGLMKTLRAKD 187

Query: 148 EEIEKIGKLNWELEERVNCLSMENQLWRDVAETNEATANALRSNLEQVLLQVNTVAEEE- 207
           +EI  IGKLN  LEE+V  L +ENQ+WRDVA++NEAT NALRSNL+QVL  V     EE 
Sbjct: 188 DEINHIGKLNLFLEEKVKSLCVENQIWRDVAQSNEATVNALRSNLQQVLAAVERNRWEEP 247

Query: 208 ----EDAESCCGSNDDGGCGGGGGVRKKRKEGDEEEERR--------CKKCWKEESCVLL 267
               +DA+SCCGSND+    G     + +  G+ ++ ++        C+ C K E+ VLL
Sbjct: 248 PTVADDAQSCCGSNDE----GDSEEERWKLAGEAQDTKKMCRVGMSMCRSCGKGEASVLL 307

Query: 268 LPCRHLCLCTVCASSLHTCPICNSTNNASVRV 286
           LPCRH+CLC+VC SSL+TCPIC S   AS+ V
Sbjct: 308 LPCRHMCLCSVCGSSLNTCPICKSPKTASLHV 331

BLAST of Bhi10G000754 vs. ExPASy Swiss-Prot
Match: O81851 (E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 8.2e-40
Identity = 108/257 (42.02%), Postives = 155/257 (60.31%), Query Frame = 0

Query: 43  KSDSGITYNLPIP-----RKRGREHHNSNRFVSYPSSHVHSQKNCGCNLYFLGEDISIQI 102
           K++SG++YN  +P     R+R  +  +SN  V   S    S      N+     ++  QI
Sbjct: 61  KAESGLSYNFTVPPLSTKRQRDFQFSDSNAPVKRRSVAFDSSSPSLINV-----ELVSQI 120

Query: 103 Q-QQQIDVDRLISQHMERVKMELEEKQKREARRILEVIEIGMMRILRSKEEEIEKIGKLN 162
           Q QQQ ++DR ++Q  E++++E+E +Q+ + R +   ++  + + L+ K++EI +I  LN
Sbjct: 121 QNQQQSEIDRFVAQQTEKLRIEIEARQQTQTRMLASAVQNVIAKKLKEKDDEIVRIRNLN 180

Query: 163 WELEERVNCLSMENQLWRDVAETNEATANALRSNLEQVLLQVNTV----AEEEEDAESCC 222
           W L+ERV  L +ENQ+WRD+A+TNEA AN LR+NL+QVL Q+ T     A  E+DAES C
Sbjct: 181 WVLQERVKSLYVENQIWRDIAQTNEANANTLRTNLDQVLAQLETFPTASAVVEDDAESSC 240

Query: 223 GSNDDGGCGGGGGVRKKRKEGDEEEERRCKKCWKEESCVLLLPCRHLCLCTVCASS--LH 282
           GS     CG GGG       G       CK+C + E+ VL+LPCRHLCLCTVC  S  L 
Sbjct: 241 GS----CCGDGGGEAVTAVGGG------CKRCGEREASVLVLPCRHLCLCTVCGGSALLR 300

Query: 283 TCPICNSTNNASVRVIM 288
           TCP+C+   NASV V M
Sbjct: 301 TCPVCDMVMNASVHVNM 302

BLAST of Bhi10G000754 vs. ExPASy Swiss-Prot
Match: Q9FHE4 (BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG1 PE=1 SV=1)

HSP 1 Score: 162.2 bits (409), Expect = 9.0e-39
Identity = 97/201 (48.26%), Postives = 132/201 (65.67%), Query Frame = 0

Query: 96  QIQQQQIDVDRLISQHMERVKMELEEKQKREARRILEVIEIGMMRILRSKEEEIEKIGKL 155
           QIQQQ  ++DR ++Q  E +++ELE +Q+ + R +   ++  +++ L++K+EEI ++GKL
Sbjct: 105 QIQQQNSEIDRFVAQQTETLRIELEARQRTQTRMLASAVQNAILKKLKAKDEEIIRMGKL 164

Query: 156 NWELEERVNCLSMENQLWRDVAETNEATANALRSNLEQVLLQVN-------TVAEEEEDA 215
           NW L+ERV  L +ENQ+WRD+A+TNEATAN LRSNLEQVL QV+        + EE +DA
Sbjct: 165 NWVLQERVKNLYVENQIWRDLAQTNEATANNLRSNLEQVLAQVDDLDAFRRPLVEEADDA 224

Query: 216 ESCCGSNDDGGCGGGGGVRKKRKEGDEEEERRCKKCWKEESCVLLLPCRHLCLCTVCASS 275
           ES CGS D      GG V      G       CK+C +  + VL+LPCRHLCLCTVC SS
Sbjct: 225 ESSCGSCD------GGDVTAVVNGG-------CKRCGELTASVLVLPCRHLCLCTVCGSS 284

Query: 276 --LHTCPICNSTNNASVRVIM 288
             L TCP+C+    ASV V M
Sbjct: 285 ALLRTCPVCDMVMTASVHVNM 292

BLAST of Bhi10G000754 vs. ExPASy Swiss-Prot
Match: Q13489 (Baculoviral IAP repeat-containing protein 3 OS=Homo sapiens OX=9606 GN=BIRC3 PE=1 SV=2)

HSP 1 Score: 53.1 bits (126), Expect = 5.9e-06
Identity = 24/52 (46.15%), Postives = 32/52 (61.54%), Query Frame = 0

Query: 236 EEERRCKKCWKEESCVLLLPCRHLCLCTVCASSLHTCPICNSTNNASVRVIM 288
           +EER CK C  +E  ++ +PC HL +C  CA SL  CPIC ST   +VR  +
Sbjct: 552 QEERTCKVCMDKEVSIVFIPCGHLVVCKDCAPSLRKCPICRSTIKGTVRTFL 603

BLAST of Bhi10G000754 vs. NCBI nr
Match: XP_038900738.1 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X2 [Benincasa hispida])

HSP 1 Score: 564.7 bits (1454), Expect = 4.6e-157
Identity = 288/288 (100.00%), Postives = 288/288 (100.00%), Query Frame = 0

Query: 1   MGYGSPLTAFNANATAVETAALFPPMDSFPAAVLPASTGVVMKSDSGITYNLPIPRKRGR 60
           MGYGSPLTAFNANATAVETAALFPPMDSFPAAVLPASTGVVMKSDSGITYNLPIPRKRGR
Sbjct: 1   MGYGSPLTAFNANATAVETAALFPPMDSFPAAVLPASTGVVMKSDSGITYNLPIPRKRGR 60

Query: 61  EHHNSNRFVSYPSSHVHSQKNCGCNLYFLGEDISIQIQQQQIDVDRLISQHMERVKMELE 120
           EHHNSNRFVSYPSSHVHSQKNCGCNLYFLGEDISIQIQQQQIDVDRLISQHMERVKMELE
Sbjct: 61  EHHNSNRFVSYPSSHVHSQKNCGCNLYFLGEDISIQIQQQQIDVDRLISQHMERVKMELE 120

Query: 121 EKQKREARRILEVIEIGMMRILRSKEEEIEKIGKLNWELEERVNCLSMENQLWRDVAETN 180
           EKQKREARRILEVIEIGMMRILRSKEEEIEKIGKLNWELEERVNCLSMENQLWRDVAETN
Sbjct: 121 EKQKREARRILEVIEIGMMRILRSKEEEIEKIGKLNWELEERVNCLSMENQLWRDVAETN 180

Query: 181 EATANALRSNLEQVLLQVNTVAEEEEDAESCCGSNDDGGCGGGGGVRKKRKEGDEEEERR 240
           EATANALRSNLEQVLLQVNTVAEEEEDAESCCGSNDDGGCGGGGGVRKKRKEGDEEEERR
Sbjct: 181 EATANALRSNLEQVLLQVNTVAEEEEDAESCCGSNDDGGCGGGGGVRKKRKEGDEEEERR 240

Query: 241 CKKCWKEESCVLLLPCRHLCLCTVCASSLHTCPICNSTNNASVRVIMP 289
           CKKCWKEESCVLLLPCRHLCLCTVCASSLHTCPICNSTNNASVRVIMP
Sbjct: 241 CKKCWKEESCVLLLPCRHLCLCTVCASSLHTCPICNSTNNASVRVIMP 288

BLAST of Bhi10G000754 vs. NCBI nr
Match: XP_038900737.1 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 [Benincasa hispida])

HSP 1 Score: 560.1 bits (1442), Expect = 1.1e-155
Identity = 288/289 (99.65%), Postives = 288/289 (99.65%), Query Frame = 0

Query: 1   MGYGSPLTAFNANATAVETAALFPPMDSFPAAVLPASTGVVMKSDSGITYNLPIPRKRGR 60
           MGYGSPLTAFNANATAVETAALFPPMDSFPAAVLPASTGVVMKSDSGITYNLPIPRKRGR
Sbjct: 1   MGYGSPLTAFNANATAVETAALFPPMDSFPAAVLPASTGVVMKSDSGITYNLPIPRKRGR 60

Query: 61  EHHNSNRFVSYPSSHVHSQKNCGCNLYFLGEDISIQIQQQQIDVDRLISQH-MERVKMEL 120
           EHHNSNRFVSYPSSHVHSQKNCGCNLYFLGEDISIQIQQQQIDVDRLISQH MERVKMEL
Sbjct: 61  EHHNSNRFVSYPSSHVHSQKNCGCNLYFLGEDISIQIQQQQIDVDRLISQHQMERVKMEL 120

Query: 121 EEKQKREARRILEVIEIGMMRILRSKEEEIEKIGKLNWELEERVNCLSMENQLWRDVAET 180
           EEKQKREARRILEVIEIGMMRILRSKEEEIEKIGKLNWELEERVNCLSMENQLWRDVAET
Sbjct: 121 EEKQKREARRILEVIEIGMMRILRSKEEEIEKIGKLNWELEERVNCLSMENQLWRDVAET 180

Query: 181 NEATANALRSNLEQVLLQVNTVAEEEEDAESCCGSNDDGGCGGGGGVRKKRKEGDEEEER 240
           NEATANALRSNLEQVLLQVNTVAEEEEDAESCCGSNDDGGCGGGGGVRKKRKEGDEEEER
Sbjct: 181 NEATANALRSNLEQVLLQVNTVAEEEEDAESCCGSNDDGGCGGGGGVRKKRKEGDEEEER 240

Query: 241 RCKKCWKEESCVLLLPCRHLCLCTVCASSLHTCPICNSTNNASVRVIMP 289
           RCKKCWKEESCVLLLPCRHLCLCTVCASSLHTCPICNSTNNASVRVIMP
Sbjct: 241 RCKKCWKEESCVLLLPCRHLCLCTVCASSLHTCPICNSTNNASVRVIMP 289

BLAST of Bhi10G000754 vs. NCBI nr
Match: XP_004145732.2 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X2 [Cucumis sativus] >KGN46823.1 hypothetical protein Csa_020941 [Cucumis sativus])

HSP 1 Score: 426.0 bits (1094), Expect = 2.6e-115
Identity = 242/328 (73.78%), Postives = 263/328 (80.18%), Query Frame = 0

Query: 1   MGYGSPLTAFNANATAVETAALFP------PMDSFP--AAVLPASTGVVMKSDSGITYNL 60
           MGYGSPLTAFNAN+TAVETAALFP      PMDSFP   AVLP STGVVMKSDSGITYNL
Sbjct: 3   MGYGSPLTAFNANSTAVETAALFPPYNSTLPMDSFPKATAVLPTSTGVVMKSDSGITYNL 62

Query: 61  PIP-RKRGREHHNS-------NRFVSYPSSHVH--SQKNCGCNLYFLGEDISIQIQQQQI 120
           PIP RKR R+HH+        NRFVSYPSS +H  SQKNCGCNLYFLGEDISIQIQQQQ+
Sbjct: 63  PIPARKRPRDHHHHHSSTSTLNRFVSYPSSQLHNNSQKNCGCNLYFLGEDISIQIQQQQM 122

Query: 121 DVDRLISQHMERVKMELEEKQKREARRILEVIEIGMMRILRSKEEEIEKIGKLNWELEER 180
           D+D LISQHME+V+ME+EEK+KREARRI+EVIE+GMMR+LRSKEEEIEK+GKLNWELEER
Sbjct: 123 DLDLLISQHMEKVRMEVEEKRKREARRIMEVIEVGMMRVLRSKEEEIEKMGKLNWELEER 182

Query: 181 VNCLSMENQLWRDVAETNEATANALRSNLEQVLL----QVNTVAEEEEDAESCCGSNDDG 240
           VNCLSMENQ+WRDVAETNEATANALR NLE+VLL    +   V  EEE AESCC      
Sbjct: 183 VNCLSMENQIWRDVAETNEATANALRRNLEEVLLLQETKTTVVVVEEEVAESCCE----- 242

Query: 241 GCGGGGGV----------RKKRKEGD--------EEEERRCKKCWKEESCVLLLPCRHLC 289
             GGGGG           +KKRK+ +        EEEERRCKKC KEESCVLLLPCRHLC
Sbjct: 243 --GGGGGAEEEDDERRMRKKKRKQEEENEEEEEAEEEERRCKKCGKEESCVLLLPCRHLC 302

BLAST of Bhi10G000754 vs. NCBI nr
Match: XP_008456341.1 (PREDICTED: probable BOI-related E3 ubiquitin-protein ligase 2 isoform X2 [Cucumis melo])

HSP 1 Score: 422.9 bits (1086), Expect = 2.2e-114
Identity = 243/331 (73.41%), Postives = 262/331 (79.15%), Query Frame = 0

Query: 1   MGYGSPLTAFNANATAVETAALFP------PMD-SFP--AAVLPASTGVVMKSDSGITYN 60
           MGYGSPLT+FNANAT +ETAALFP      PMD SFP  A VLP STGVVMKSDSGITYN
Sbjct: 1   MGYGSPLTSFNANATPMETAALFPPYNSTLPMDHSFPKAAPVLPTSTGVVMKSDSGITYN 60

Query: 61  LPIPRKRGREHHNS-------------NRFVSYPSSHV--HSQKNCGCNLYFLGEDISIQ 120
           LPIPRKR R+HH+              NRFVSYPSS V  +SQKNCGCNLYFLGEDISIQ
Sbjct: 61  LPIPRKRPRDHHHHHHHHPHSSTSNTLNRFVSYPSSQVQNNSQKNCGCNLYFLGEDISIQ 120

Query: 121 IQQQQIDVDRLISQHMERVKMELEEKQKREARRILEVIEIGMMRILRSKEEEIEKIGKLN 180
           IQQQQ+D+D LISQHME+V+ME+EEK+KREARRI+EVIE+GMMRILRSKEEEIEK+GKLN
Sbjct: 121 IQQQQMDLDLLISQHMEKVRMEVEEKRKREARRIMEVIELGMMRILRSKEEEIEKMGKLN 180

Query: 181 WELEERVNCLSMENQLWRDVAETNEATANALRSNLEQVLL---QVNTVAEEEEDAESCCG 240
           WELEERVNCLSMENQ+WRDVAETNEATANALR NLE+VLL   +   V  EEE AESCC 
Sbjct: 181 WELEERVNCLSMENQIWRDVAETNEATANALRRNLEEVLLLQEKTTVVVVEEEVAESCCE 240

Query: 241 SNDDGGCGGGGGV---------RKKRKE-------GDEEEERRCKKCWKEESCVLLLPCR 289
                  GGGGG          +KKRKE       G EEEERRCKKC KEESCVLLLPCR
Sbjct: 241 -------GGGGGTDEDDERRMRKKKRKEEEDGGGGGGEEEERRCKKCGKEESCVLLLPCR 300

BLAST of Bhi10G000754 vs. NCBI nr
Match: XP_011656988.1 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 [Cucumis sativus])

HSP 1 Score: 421.4 bits (1082), Expect = 6.3e-114
Identity = 242/329 (73.56%), Postives = 263/329 (79.94%), Query Frame = 0

Query: 1   MGYGSPLTAFNANATAVETAALFP------PMDSFP--AAVLPASTGVVMKSDSGITYNL 60
           MGYGSPLTAFNAN+TAVETAALFP      PMDSFP   AVLP STGVVMKSDSGITYNL
Sbjct: 3   MGYGSPLTAFNANSTAVETAALFPPYNSTLPMDSFPKATAVLPTSTGVVMKSDSGITYNL 62

Query: 61  PIP-RKRGREHHNS-------NRFVSYPSSHVH--SQKNCGCNLYFLGEDISIQIQQQQI 120
           PIP RKR R+HH+        NRFVSYPSS +H  SQKNCGCNLYFLGEDISIQIQQQQ+
Sbjct: 63  PIPARKRPRDHHHHHSSTSTLNRFVSYPSSQLHNNSQKNCGCNLYFLGEDISIQIQQQQM 122

Query: 121 DVDRLISQH-MERVKMELEEKQKREARRILEVIEIGMMRILRSKEEEIEKIGKLNWELEE 180
           D+D LISQH ME+V+ME+EEK+KREARRI+EVIE+GMMR+LRSKEEEIEK+GKLNWELEE
Sbjct: 123 DLDLLISQHQMEKVRMEVEEKRKREARRIMEVIEVGMMRVLRSKEEEIEKMGKLNWELEE 182

Query: 181 RVNCLSMENQLWRDVAETNEATANALRSNLEQVLL----QVNTVAEEEEDAESCCGSNDD 240
           RVNCLSMENQ+WRDVAETNEATANALR NLE+VLL    +   V  EEE AESCC     
Sbjct: 183 RVNCLSMENQIWRDVAETNEATANALRRNLEEVLLLQETKTTVVVVEEEVAESCCE---- 242

Query: 241 GGCGGGGGV----------RKKRKEGD--------EEEERRCKKCWKEESCVLLLPCRHL 289
              GGGGG           +KKRK+ +        EEEERRCKKC KEESCVLLLPCRHL
Sbjct: 243 ---GGGGGAEEEDDERRMRKKKRKQEEENEEEEEAEEEERRCKKCGKEESCVLLLPCRHL 302

BLAST of Bhi10G000754 vs. ExPASy TrEMBL
Match: A0A0A0KBB9 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G139230 PE=4 SV=1)

HSP 1 Score: 426.0 bits (1094), Expect = 1.2e-115
Identity = 242/328 (73.78%), Postives = 263/328 (80.18%), Query Frame = 0

Query: 1   MGYGSPLTAFNANATAVETAALFP------PMDSFP--AAVLPASTGVVMKSDSGITYNL 60
           MGYGSPLTAFNAN+TAVETAALFP      PMDSFP   AVLP STGVVMKSDSGITYNL
Sbjct: 3   MGYGSPLTAFNANSTAVETAALFPPYNSTLPMDSFPKATAVLPTSTGVVMKSDSGITYNL 62

Query: 61  PIP-RKRGREHHNS-------NRFVSYPSSHVH--SQKNCGCNLYFLGEDISIQIQQQQI 120
           PIP RKR R+HH+        NRFVSYPSS +H  SQKNCGCNLYFLGEDISIQIQQQQ+
Sbjct: 63  PIPARKRPRDHHHHHSSTSTLNRFVSYPSSQLHNNSQKNCGCNLYFLGEDISIQIQQQQM 122

Query: 121 DVDRLISQHMERVKMELEEKQKREARRILEVIEIGMMRILRSKEEEIEKIGKLNWELEER 180
           D+D LISQHME+V+ME+EEK+KREARRI+EVIE+GMMR+LRSKEEEIEK+GKLNWELEER
Sbjct: 123 DLDLLISQHMEKVRMEVEEKRKREARRIMEVIEVGMMRVLRSKEEEIEKMGKLNWELEER 182

Query: 181 VNCLSMENQLWRDVAETNEATANALRSNLEQVLL----QVNTVAEEEEDAESCCGSNDDG 240
           VNCLSMENQ+WRDVAETNEATANALR NLE+VLL    +   V  EEE AESCC      
Sbjct: 183 VNCLSMENQIWRDVAETNEATANALRRNLEEVLLLQETKTTVVVVEEEVAESCCE----- 242

Query: 241 GCGGGGGV----------RKKRKEGD--------EEEERRCKKCWKEESCVLLLPCRHLC 289
             GGGGG           +KKRK+ +        EEEERRCKKC KEESCVLLLPCRHLC
Sbjct: 243 --GGGGGAEEEDDERRMRKKKRKQEEENEEEEEAEEEERRCKKCGKEESCVLLLPCRHLC 302

BLAST of Bhi10G000754 vs. ExPASy TrEMBL
Match: A0A1S3C2Z7 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103496308 PE=4 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 1.0e-114
Identity = 243/331 (73.41%), Postives = 262/331 (79.15%), Query Frame = 0

Query: 1   MGYGSPLTAFNANATAVETAALFP------PMD-SFP--AAVLPASTGVVMKSDSGITYN 60
           MGYGSPLT+FNANAT +ETAALFP      PMD SFP  A VLP STGVVMKSDSGITYN
Sbjct: 1   MGYGSPLTSFNANATPMETAALFPPYNSTLPMDHSFPKAAPVLPTSTGVVMKSDSGITYN 60

Query: 61  LPIPRKRGREHHNS-------------NRFVSYPSSHV--HSQKNCGCNLYFLGEDISIQ 120
           LPIPRKR R+HH+              NRFVSYPSS V  +SQKNCGCNLYFLGEDISIQ
Sbjct: 61  LPIPRKRPRDHHHHHHHHPHSSTSNTLNRFVSYPSSQVQNNSQKNCGCNLYFLGEDISIQ 120

Query: 121 IQQQQIDVDRLISQHMERVKMELEEKQKREARRILEVIEIGMMRILRSKEEEIEKIGKLN 180
           IQQQQ+D+D LISQHME+V+ME+EEK+KREARRI+EVIE+GMMRILRSKEEEIEK+GKLN
Sbjct: 121 IQQQQMDLDLLISQHMEKVRMEVEEKRKREARRIMEVIELGMMRILRSKEEEIEKMGKLN 180

Query: 181 WELEERVNCLSMENQLWRDVAETNEATANALRSNLEQVLL---QVNTVAEEEEDAESCCG 240
           WELEERVNCLSMENQ+WRDVAETNEATANALR NLE+VLL   +   V  EEE AESCC 
Sbjct: 181 WELEERVNCLSMENQIWRDVAETNEATANALRRNLEEVLLLQEKTTVVVVEEEVAESCCE 240

Query: 241 SNDDGGCGGGGGV---------RKKRKE-------GDEEEERRCKKCWKEESCVLLLPCR 289
                  GGGGG          +KKRKE       G EEEERRCKKC KEESCVLLLPCR
Sbjct: 241 -------GGGGGTDEDDERRMRKKKRKEEEDGGGGGGEEEERRCKKCGKEESCVLLLPCR 300

BLAST of Bhi10G000754 vs. ExPASy TrEMBL
Match: A0A1S3C331 (probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496308 PE=4 SV=1)

HSP 1 Score: 418.3 bits (1074), Expect = 2.6e-113
Identity = 243/332 (73.19%), Postives = 262/332 (78.92%), Query Frame = 0

Query: 1   MGYGSPLTAFNANATAVETAALFP------PMD-SFP--AAVLPASTGVVMKSDSGITYN 60
           MGYGSPLT+FNANAT +ETAALFP      PMD SFP  A VLP STGVVMKSDSGITYN
Sbjct: 1   MGYGSPLTSFNANATPMETAALFPPYNSTLPMDHSFPKAAPVLPTSTGVVMKSDSGITYN 60

Query: 61  LPIPRKRGREHHNS-------------NRFVSYPSSHV--HSQKNCGCNLYFLGEDISIQ 120
           LPIPRKR R+HH+              NRFVSYPSS V  +SQKNCGCNLYFLGEDISIQ
Sbjct: 61  LPIPRKRPRDHHHHHHHHPHSSTSNTLNRFVSYPSSQVQNNSQKNCGCNLYFLGEDISIQ 120

Query: 121 IQQQQIDVDRLISQH-MERVKMELEEKQKREARRILEVIEIGMMRILRSKEEEIEKIGKL 180
           IQQQQ+D+D LISQH ME+V+ME+EEK+KREARRI+EVIE+GMMRILRSKEEEIEK+GKL
Sbjct: 121 IQQQQMDLDLLISQHQMEKVRMEVEEKRKREARRIMEVIELGMMRILRSKEEEIEKMGKL 180

Query: 181 NWELEERVNCLSMENQLWRDVAETNEATANALRSNLEQVLL---QVNTVAEEEEDAESCC 240
           NWELEERVNCLSMENQ+WRDVAETNEATANALR NLE+VLL   +   V  EEE AESCC
Sbjct: 181 NWELEERVNCLSMENQIWRDVAETNEATANALRRNLEEVLLLQEKTTVVVVEEEVAESCC 240

Query: 241 GSNDDGGCGGGGGV---------RKKRKE-------GDEEEERRCKKCWKEESCVLLLPC 289
                   GGGGG          +KKRKE       G EEEERRCKKC KEESCVLLLPC
Sbjct: 241 E-------GGGGGTDEDDERRMRKKKRKEEEDGGGGGGEEEERRCKKCGKEESCVLLLPC 300

BLAST of Bhi10G000754 vs. ExPASy TrEMBL
Match: A0A6J1FUW1 (probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111448331 PE=4 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 1.1e-108
Identity = 222/315 (70.48%), Postives = 245/315 (77.78%), Query Frame = 0

Query: 1   MGYGSPLTAFNANATAVETAALFP----PMDSFPAAV------LPASTGVVMKSDSGITY 60
           MGYG+PLT F+AN  A ET  L P     +DSFP AV        ASTGVVMKSDS I+Y
Sbjct: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKAVGAMPASASASTGVVMKSDSAISY 60

Query: 61  NLPIPRKRGREHHNSNRFVSYPSSHVHSQKNCGCNLYFLGEDISIQIQQQQIDVDRLISQ 120
           NLPIPRKRGR+H   NRF+SYPSSH HSQKNCG NLYFLGEDISI +QQQQ+D++RLISQ
Sbjct: 61  NLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRNLYFLGEDISILVQQQQLDLERLISQ 120

Query: 121 HMERVKMELEEKQKREARRILEVIEIGMMRILRSKEEEIEKIGKLNWELEERVNCLSMEN 180
           HME+V+ME+EEK+KREARRI+E IE+GMM ILRSKEEEIEK+GKLNWELEERVN L MEN
Sbjct: 121 HMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN 180

Query: 181 QLWRDVAETNEATANALRSNLEQVLLQV--NTVAEEEEDAESCCGSNDDGGCGGGGGV-- 240
           Q+WRDVAETNEATANALRS+LE VLL V      EEEE AESCCGSN       GG +  
Sbjct: 181 QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSN-------GGDMEE 240

Query: 241 -------------RKKRKEGDEEEERRCKKCWKEESCVLLLPCRHLCLCTVCASSLHTCP 289
                        RK R++  EEE+RRCK CWKEESCVLLLPCRHLCLCTVC SSLH CP
Sbjct: 241 EEEEEVEEERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCP 300

BLAST of Bhi10G000754 vs. ExPASy TrEMBL
Match: A0A6J1JCV5 (probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111483871 PE=4 SV=1)

HSP 1 Score: 402.5 bits (1033), Expect = 1.5e-108
Identity = 221/315 (70.16%), Postives = 245/315 (77.78%), Query Frame = 0

Query: 1   MGYGSPLTAFNANATAVETAALFP----PMDSFPAAV------LPASTGVVMKSDSGITY 60
           MGYG+PLT F+AN  A ET  L P     +DSFP A+        ASTGVVMKSDSGI+Y
Sbjct: 1   MGYGAPLTGFSANTAAAETTLLPPYNSVAIDSFPKALGAMPASASASTGVVMKSDSGISY 60

Query: 61  NLPIPRKRGREHHNSNRFVSYPSSHVHSQKNCGCNLYFLGEDISIQIQQQQIDVDRLISQ 120
           NLPIPRKRGR+H   NRF+SYPSSH HSQKNCG N YFLGEDISI +QQQQ+D++RLISQ
Sbjct: 61  NLPIPRKRGRDHSTINRFLSYPSSHAHSQKNCGRNFYFLGEDISILVQQQQLDLERLISQ 120

Query: 121 HMERVKMELEEKQKREARRILEVIEIGMMRILRSKEEEIEKIGKLNWELEERVNCLSMEN 180
           HME+V+ME+EEK+KREARRI+E IE+GMM ILRSKEEEIEK+GKLNWELEERVN L MEN
Sbjct: 121 HMEKVRMEMEEKRKREARRIMEAIEVGMMGILRSKEEEIEKMGKLNWELEERVNSLCMEN 180

Query: 181 QLWRDVAETNEATANALRSNLEQVLLQV--NTVAEEEEDAESCCGSNDDGGCGGGGGV-- 240
           Q+WRDVAETNEATANALRS+LE VLL V      EEEE AESCCGSN       GG +  
Sbjct: 181 QIWRDVAETNEATANALRSDLELVLLDVKKEEEVEEEEKAESCCGSN-------GGDMEE 240

Query: 241 -------------RKKRKEGDEEEERRCKKCWKEESCVLLLPCRHLCLCTVCASSLHTCP 289
                        RK R++  EEE+RRCK CWKEESCVLLLPCRHLCLCTVC SSLH CP
Sbjct: 241 EEEEEVEEERRKGRKTRRKEKEEEDRRCKNCWKEESCVLLLPCRHLCLCTVCGSSLHHCP 300

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT1G79110.21.5e-5746.56zinc ion binding [more]
AT1G79110.16.4e-5646.13zinc ion binding [more]
AT3G12920.14.1e-5545.96SBP (S-ribonuclease binding protein) family protein [more]
AT4G19700.15.8e-4142.02SBP (S-ribonuclease binding protein) family protein [more]
AT5G45100.16.4e-4048.26SBP (S-ribonuclease binding protein) family protein [more]
Match NameE-valueIdentityDescription
F4IDI69.0e-5546.13Probable BOI-related E3 ubiquitin-protein ligase 2 OS=Arabidopsis thaliana OX=37... [more]
Q9LDD15.8e-5445.96Probable BOI-related E3 ubiquitin-protein ligase 3 OS=Arabidopsis thaliana OX=37... [more]
O818518.2e-4042.02E3 ubiquitin-protein ligase BOI OS=Arabidopsis thaliana OX=3702 GN=BOI PE=1 SV=1[more]
Q9FHE49.0e-3948.26BOI-related E3 ubiquitin-protein ligase 1 OS=Arabidopsis thaliana OX=3702 GN=BRG... [more]
Q134895.9e-0646.15Baculoviral IAP repeat-containing protein 3 OS=Homo sapiens OX=9606 GN=BIRC3 PE=... [more]
Match NameE-valueIdentityDescription
XP_038900738.14.6e-157100.00probable BOI-related E3 ubiquitin-protein ligase 2 isoform X2 [Benincasa hispida... [more]
XP_038900737.11.1e-15599.65probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 [Benincasa hispida... [more]
XP_004145732.22.6e-11573.78probable BOI-related E3 ubiquitin-protein ligase 2 isoform X2 [Cucumis sativus] ... [more]
XP_008456341.12.2e-11473.41PREDICTED: probable BOI-related E3 ubiquitin-protein ligase 2 isoform X2 [Cucumi... [more]
XP_011656988.16.3e-11473.56probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A0A0KBB91.2e-11573.78RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G139230 P... [more]
A0A1S3C2Z71.0e-11473.41probable BOI-related E3 ubiquitin-protein ligase 2 isoform X2 OS=Cucumis melo OX... [more]
A0A1S3C3312.6e-11373.19probable BOI-related E3 ubiquitin-protein ligase 2 isoform X1 OS=Cucumis melo OX... [more]
A0A6J1FUW11.1e-10870.48probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 OS=Cucurbita mosch... [more]
A0A6J1JCV51.5e-10870.16probable BOI-related E3 ubiquitin-protein ligase 3 isoform X2 OS=Cucurbita maxim... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 142..162
NoneNo IPR availablePFAMPF13920zf-C3HC4_3coord: 238..277
e-value: 1.1E-8
score: 34.7
NoneNo IPR availablePANTHERPTHR42647:SF12BOI-RELATED E3 UBIQUITIN-PROTEIN LIGASE 2-RELATEDcoord: 6..287
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 225..288
e-value: 7.3E-13
score: 49.7
IPR017066E3 ubiquitin-protein ligase BOI-likePANTHERPTHR42647SBP (S-RIBONUCLEASE BINDING PROTEIN) FAMILY PROTEINcoord: 6..287
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 241..276
score: 9.420654

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi10M000754Bhi10M000754mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
biological_process GO:0043067 regulation of programmed cell death
molecular_function GO:0046872 metal ion binding
molecular_function GO:0004842 ubiquitin-protein transferase activity