Bhi10G000663 (gene) Wax gourd (B227) v1

Overview
NameBhi10G000663
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionGDSL esterase/lipase
Locationchr10: 17302118 .. 17303818 (-)
RNA-Seq ExpressionBhi10G000663
SyntenyBhi10G000663
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCACGAGCTAATTACCTCATTGTTGCTCTCTCACTCCACGTAACCTGGCTGCTATTTCTTAGCAAACCATGCATCGCTTTGACACCTGAAACTTCTCCATCTTTTCCTGCCATTCTCATATTTGGTGATTCTACGGTCGACACCGGAAATAACAACTTCATCCCCACTATTTTCAAGGCTAATTACTCTCCATACGGCAAAGATTTTCCTGGCCATGTTGCTACTGGAAGGTTCAGTGATGGGAAACTCATTCCTGACATGGTGGCATCTAAGTTGGGGATAAAGGAGCTTGTTCCTCCATTTCTGGATCCTGAACTGTCGGATGATGATGTGAAAACAGGGGTCAGTTTTGCATCCGCCGGCACAGGACTCGATGATCGAACAGCCGCTATATCTACAGTCATTCCTGCGATGAAACAAATTGGTATGTTCAAGAACTACATTCAAAGGCTTCAAAGAATTGTGGGTGTGGATGAAAGTAAGAGGATTATTGGCAGTGCTTTGGCTGTCATTAGTGTAGGAACCAATGACTTAACCTTCAATTTCTACGACCTTCCAACCAGGCAGTTGCAATTCAATATTAGTGATTACCAAGATTTTCTACAGAATAGACTACAAAGCTTGATCAAGGTAAATGATATTCAATATAATATGCACTCATTAATCTTCACCGATATTTGACACTTATAAAAAAAATCTTGATTTCAAAAATACCCTTTTTTTTCTTTTTTCCAAAGTTGCAACGTTATATTTGACCTCAAATAAATATTTTAGAACTAGAAATCCTTTGAAATTTTGGATATAATTGGGATCCAAAATTGGGTGGCGTTGAGCTAAAATAGAAATTTAGATTGTCACACCATTCCAAGTAGTTCTAAAATGTTCGTAAAAGGTGAAAGATACATTGTAAGTTTTGAAAGAATAAGGGTATTTTAAAAATAAATAGCAAAGTTAAAGAGTGTTGTTTATAATTTAGCCTTGACATAATAATCACCAATGCATCAGTTACAAGTTCCACATCTTCAATTTTGTATGCTTCAGTCCTATTCTTGAAAAGTTTTTAACCTTCAAAGTTTGATCATTCTAATAGACTTATCAACTACTCCCTGCTTCAAACGAACACTTGTTTAACTCTGTTTTACAATGGACAGGAAATTTACCAGCTTGGATGTCGCACTATAGTGGTCGCTGGCCTCCCTCCAATTGGTTGTCTTCCAATTCAAGAGACCATATCATTCCCAATTCCTCTAAATCGAAAATGTTTGGAAGATCAAAATACAGATTCCGAAGCCTACAATCAGAAGCTTTCAAAGCTGTTGGGCAATTTACAATCACAGCTACTAGGAAGCACAATTTTGTATGCTGACATTTACACTCCTCTCGTGGACATGATTAATAATCCCCAAAAATATGGTAAGCCGGTATAAATAATTAAGGGAAATCAAGTTATGAACAAAAAAATCAATGTAATTGACCTGAAATTTGTTTGGGGCATTTCGTCGAACAGGTTTTGAGCAGACGAACATTGGTTGTTGTGGAACTGGATTGGCTGAAGCAGGGCCTCTATGTAATGCATCAACTCCAACGTGTGAAGACCCATCAAAATTTATGTTCTGGGATAGCATTCATCCCACTGAAGCAACCTACAAATTCATCACAGAGTCGCTTCTTAAGCAATTTTCCGATCACTTGAATTAG

mRNA sequence

ATGGCACGAGCTAATTACCTCATTGTTGCTCTCTCACTCCACGTAACCTGGCTGCTATTTCTTAGCAAACCATGCATCGCTTTGACACCTGAAACTTCTCCATCTTTTCCTGCCATTCTCATATTTGGTGATTCTACGGTCGACACCGGAAATAACAACTTCATCCCCACTATTTTCAAGGCTAATTACTCTCCATACGGCAAAGATTTTCCTGGCCATGTTGCTACTGGAAGGTTCAGTGATGGGAAACTCATTCCTGACATGGTGGCATCTAAGTTGGGGATAAAGGAGCTTGTTCCTCCATTTCTGGATCCTGAACTGTCGGATGATGATGTGAAAACAGGGGTCAGTTTTGCATCCGCCGGCACAGGACTCGATGATCGAACAGCCGCTATATCTACAGTCATTCCTGCGATGAAACAAATTGGTATGTTCAAGAACTACATTCAAAGGCTTCAAAGAATTGTGGGTGTGGATGAAAGTAAGAGGATTATTGGCAGTGCTTTGGCTGTCATTAGTGTAGGAACCAATGACTTAACCTTCAATTTCTACGACCTTCCAACCAGGCAGTTGCAATTCAATATTAGTGATTACCAAGATTTTCTACAGAATAGACTACAAAGCTTGATCAAGGAAATTTACCAGCTTGGATGTCGCACTATAGTGGTCGCTGGCCTCCCTCCAATTGGTTGTCTTCCAATTCAAGAGACCATATCATTCCCAATTCCTCTAAATCGAAAATGTTTGGAAGATCAAAATACAGATTCCGAAGCCTACAATCAGAAGCTTTCAAAGCTGTTGGGCAATTTACAATCACAGCTACTAGGAAGCACAATTTTGTATGCTGACATTTACACTCCTCTCGTGGACATGATTAATAATCCCCAAAAATATGGTTTTGAGCAGACGAACATTGGTTGTTGTGGAACTGGATTGGCTGAAGCAGGGCCTCTATGTAATGCATCAACTCCAACGTGTGAAGACCCATCAAAATTTATGTTCTGGGATAGCATTCATCCCACTGAAGCAACCTACAAATTCATCACAGAGTCGCTTCTTAAGCAATTTTCCGATCACTTGAATTAG

Coding sequence (CDS)

ATGGCACGAGCTAATTACCTCATTGTTGCTCTCTCACTCCACGTAACCTGGCTGCTATTTCTTAGCAAACCATGCATCGCTTTGACACCTGAAACTTCTCCATCTTTTCCTGCCATTCTCATATTTGGTGATTCTACGGTCGACACCGGAAATAACAACTTCATCCCCACTATTTTCAAGGCTAATTACTCTCCATACGGCAAAGATTTTCCTGGCCATGTTGCTACTGGAAGGTTCAGTGATGGGAAACTCATTCCTGACATGGTGGCATCTAAGTTGGGGATAAAGGAGCTTGTTCCTCCATTTCTGGATCCTGAACTGTCGGATGATGATGTGAAAACAGGGGTCAGTTTTGCATCCGCCGGCACAGGACTCGATGATCGAACAGCCGCTATATCTACAGTCATTCCTGCGATGAAACAAATTGGTATGTTCAAGAACTACATTCAAAGGCTTCAAAGAATTGTGGGTGTGGATGAAAGTAAGAGGATTATTGGCAGTGCTTTGGCTGTCATTAGTGTAGGAACCAATGACTTAACCTTCAATTTCTACGACCTTCCAACCAGGCAGTTGCAATTCAATATTAGTGATTACCAAGATTTTCTACAGAATAGACTACAAAGCTTGATCAAGGAAATTTACCAGCTTGGATGTCGCACTATAGTGGTCGCTGGCCTCCCTCCAATTGGTTGTCTTCCAATTCAAGAGACCATATCATTCCCAATTCCTCTAAATCGAAAATGTTTGGAAGATCAAAATACAGATTCCGAAGCCTACAATCAGAAGCTTTCAAAGCTGTTGGGCAATTTACAATCACAGCTACTAGGAAGCACAATTTTGTATGCTGACATTTACACTCCTCTCGTGGACATGATTAATAATCCCCAAAAATATGGTTTTGAGCAGACGAACATTGGTTGTTGTGGAACTGGATTGGCTGAAGCAGGGCCTCTATGTAATGCATCAACTCCAACGTGTGAAGACCCATCAAAATTTATGTTCTGGGATAGCATTCATCCCACTGAAGCAACCTACAAATTCATCACAGAGTCGCTTCTTAAGCAATTTTCCGATCACTTGAATTAG

Protein sequence

MARANYLIVALSLHVTWLLFLSKPCIALTPETSPSFPAILIFGDSTVDTGNNNFIPTIFKANYSPYGKDFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDPELSDDDVKTGVSFASAGTGLDDRTAAISTVIPAMKQIGMFKNYIQRLQRIVGVDESKRIIGSALAVISVGTNDLTFNFYDLPTRQLQFNISDYQDFLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETISFPIPLNRKCLEDQNTDSEAYNQKLSKLLGNLQSQLLGSTILYADIYTPLVDMINNPQKYGFEQTNIGCCGTGLAEAGPLCNASTPTCEDPSKFMFWDSIHPTEATYKFITESLLKQFSDHLN
Homology
BLAST of Bhi10G000663 vs. TAIR 10
Match: AT2G30310.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 374.4 bits (960), Expect = 1.0e-103
Identity = 187/325 (57.54%), Postives = 228/325 (70.15%), Query Frame = 0

Query: 26  IALTPETSPSFPAILIFGDSTVDTGNNNF-IPTIFKANYSPYGKDFPGHVATGRFSDGKL 85
           +A    T P FPAILIFGDSTVDTGNNN+   TIFKA + PYG D PGH A GR+S+GK+
Sbjct: 22  VAANATTQPLFPAILIFGDSTVDTGNNNYHSQTIFKAKHLPYGVDLPGHEANGRYSNGKV 81

Query: 86  IPDMVASKLGIKELVPPFLDPELSDDDVKTGVSFASAGTGLDDRTAAISTVIPAMKQIGM 145
           I D++ASKL IKELVPPFL P +S  D+ TGVSFASAG G DDR++  S  IP  +Q  M
Sbjct: 82  ISDVIASKLNIKELVPPFLQPNISHQDIVTGVSFASAGAGYDDRSSLSSKAIPVSQQPSM 141

Query: 146 FKNYIQRLQRIVGVDESKRIIGSALAVISVGTNDLTFNFYDLPTRQLQF-NISDYQDFLQ 205
           FKNYI RL+ IVG  ++  II +AL VIS G ND   NFYD+PTR+L++  I  YQ+F+ 
Sbjct: 142 FKNYIARLKGIVGDKKAMEIINNALVVISAGPNDFILNFYDIPTRRLEYPTIHGYQEFIL 201

Query: 206 NRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETISFPIPLNRKCLEDQNTDSEAYNQKL 265
            RL   ++E+Y LGCR IVV GLPP+GCLPIQ T      L R C+E +N DS  YNQKL
Sbjct: 202 KRLDGFVRELYSLGCRNIVVGGLPPMGCLPIQMTAKMRNIL-RFCVEQENKDSVLYNQKL 261

Query: 266 SKLLGNLQSQLLGSTILYADIYTPLVDMINNPQKYGFEQTNIGCCGTGLAEAGPLCNAST 325
            K L  +Q+ L GS  LYA++Y PL+DMI NP KYGF++T  GCCGTG  E   +CN  T
Sbjct: 262 VKKLPEIQASLPGSNFLYANVYDPLMDMIQNPSKYGFKETKKGCCGTGYLETTFMCNPLT 321

Query: 326 PTCEDPSKFMFWDSIHPTEATYKFI 349
            TC + S  +FWDSIHP+EA Y +I
Sbjct: 322 KTCPNHSDHLFWDSIHPSEAAYNYI 345

BLAST of Bhi10G000663 vs. TAIR 10
Match: AT2G24560.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 370.9 bits (951), Expect = 1.1e-102
Identity = 187/346 (54.05%), Postives = 246/346 (71.10%), Query Frame = 0

Query: 9   VALSLHVTWLLFLS---KPCIALTPETS-PSFPAILIFGDSTVDTGNNNF-IPTIFKANY 68
           ++ S  +T+ LF++     C A T  TS P FPAILIFGDSTVDTGNNN+   TIFKA +
Sbjct: 1   MSTSKTITFTLFIAALLSSCDAATNATSQPLFPAILIFGDSTVDTGNNNYHSQTIFKAKH 60

Query: 69  SPYGKDFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDPELSDDDVKTGVSFASAGT 128
            PYG D P H A+GRF++GK+  D++A+KL IK+ VPPFL P LSD ++ TGV FASAG 
Sbjct: 61  LPYGIDLPNHKASGRFTNGKIFSDIIATKLNIKQFVPPFLQPNLSDQEIVTGVCFASAGA 120

Query: 129 GLDDRTAAISTVIPAMKQIGMFKNYIQRLQRIVGVDESKRIIGSALAVISVGTNDLTFNF 188
           G DD T+  +  I  + Q  MFKNYI RL+ IVG  ++  II +AL VIS G ND   N+
Sbjct: 121 GYDDHTSLSTQAIRVLDQQKMFKNYIARLKSIVGDKKAMEIIKNALVVISAGPNDFILNY 180

Query: 189 YDLPTRQLQF-NISDYQDFLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETISFPI 248
           YD+P+R+L+F +IS YQDF+  RL + ++E+Y LGCR I+V GLPP+GCLPIQ T  F  
Sbjct: 181 YDIPSRRLEFPHISGYQDFVLQRLDNFVRELYSLGCRKIMVGGLPPMGCLPIQMTAKFRN 240

Query: 249 PLNRKCLEDQNTDSEAYNQKLSKLLGNLQSQLLGSTILYADIYTPLVDMINNPQKYGFEQ 308
            L R CLE +N DS  YNQKL  LL  +++ L GS ILY+++Y P++DM+ NP KYGF++
Sbjct: 241 AL-RFCLEQENRDSVLYNQKLQNLLPQIEASLTGSKILYSNVYDPMMDMMQNPSKYGFKE 300

Query: 309 TNIGCCGTGLAEAGPLCNASTPTCEDPSKFMFWDSIHPTEATYKFI 349
           T  GCCGTG  E   +CNA +PTC + S+F+F+DSIHP+EATY ++
Sbjct: 301 TKRGCCGTGHLETSFMCNAFSPTCRNHSEFLFFDSIHPSEATYNYM 345

BLAST of Bhi10G000663 vs. TAIR 10
Match: AT2G30220.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 366.3 bits (939), Expect = 2.8e-101
Identity = 183/338 (54.14%), Postives = 228/338 (67.46%), Query Frame = 0

Query: 25  CIALTPETSPSFPAILIFGDSTVDTGNNNFI-PTIFKANYSPYGKDFPGHVATGRFSDGK 84
           C A    T P FPAILIFGDST DTGNNN+    +FKAN+ PYG D PGH A GRFS+GK
Sbjct: 20  CNADANTTQPLFPAILIFGDSTADTGNNNYYSQAVFKANHLPYGVDLPGHEANGRFSNGK 79

Query: 85  LIPDMVASKLGIKELVPPFLDPELSDDDVKTGVSFASAGTGLDDRTAAISTVIPAMKQIG 144
           LI D++++KL IKE VPPFL P +SD D+ TGV FASAG G DD T+  S  IP  +Q  
Sbjct: 80  LISDVISTKLNIKEFVPPFLQPNISDQDIVTGVCFASAGAGYDDETSLSSKAIPVSQQPS 139

Query: 145 MFKNYIQRLQRIVGVDESKRIIGSALAVISVGTNDLTFNFYDLPTRQLQF-NISDYQDFL 204
           MFKNYI RL+ IVG  ++  II +AL VIS G ND   NFYD+P R+L++  I  YQDF+
Sbjct: 140 MFKNYIARLKGIVGDKKAMEIINNALVVISAGPNDFILNFYDIPIRRLEYPTIYGYQDFV 199

Query: 205 QNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETISFPIPLNRKCLEDQNTDSEAYNQK 264
             RL   ++E+Y LGCR I+V GLPP+GCLPIQ T      L   C+E +N DS  YNQK
Sbjct: 200 LKRLDGFVRELYSLGCRNILVGGLPPMGCLPIQLTAKLRTILG-ICVEQENKDSILYNQK 259

Query: 265 LSKLLGNLQSQLLGSTILYADIYTPLVDMINNPQKYGFEQTNIGCCGTGLAEAGPLCNAS 324
           L K L  +Q+ L GS  LYA++Y P++DMI NP KYGF++T  GCCGTG  E   LC + 
Sbjct: 260 LVKKLPEIQASLPGSKFLYANVYDPVMDMIRNPSKYGFKETKKGCCGTGYLETSFLCTSL 319

Query: 325 TPTCEDPSKFMFWDSIHPTEATYKFITESLLKQFSDHL 361
           + TC + S  +FWDSIHP+EA YK++   +  Q  + L
Sbjct: 320 SKTCPNHSDHLFWDSIHPSEAAYKYLGNFIDAQIQEWL 356

BLAST of Bhi10G000663 vs. TAIR 10
Match: AT1G06990.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 363.6 bits (932), Expect = 1.8e-100
Identity = 164/320 (51.25%), Postives = 227/320 (70.94%), Query Frame = 0

Query: 36  FPAILIFGDSTVDTGNNNFIPTIFKANYSPYGKDFPGHVATGRFSDGKLIPDMVASKLGI 95
           FPAIL+FGDST+DTGNNN+I T  +AN+ PYG +FPGH ATGRFS+GKLIPD +AS +GI
Sbjct: 35  FPAILVFGDSTIDTGNNNYIKTYIRANFPPYGCNFPGHNATGRFSNGKLIPDFIASLMGI 94

Query: 96  KELVPPFLDPELSDDDVKTGVSFASAGTGLDDRTAAISTVIPAMKQIGMFKNYIQRLQRI 155
           K+ VPPFLDP LSD D+ TGV FASAG+G D+ T   ++ +   KQ  M ++Y++RL +I
Sbjct: 95  KDTVPPFLDPHLSDSDIITGVCFASAGSGYDNLTDRATSTLSVDKQADMLRSYVERLSQI 154

Query: 156 VGVDESKRIIGSALAVISVGTNDLTFNFYDLPTRQLQFNISDYQDFLQNRLQSLIKEIYQ 215
           VG +++  I+  AL ++S GTND   N YD P+R+ +  +  YQ F+ + + + ++E+Y 
Sbjct: 155 VGDEKAASIVSEALVIVSSGTNDFNLNLYDTPSRRQKLGVDGYQSFILSNVHNFVQELYD 214

Query: 216 LGCRTIVVAGLPPIGCLPIQETISFPIPLNRKCLEDQNTDSEAYNQKLSKLLGNLQSQLL 275
           +GCR I+V GLPP+GCLPIQ T++      R+C++ QN+DS+ +NQKL   L  +QS L 
Sbjct: 215 IGCRKIMVLGLPPVGCLPIQMTMAMQKQNERRCIDKQNSDSQEFNQKLKNSLTEMQSNLT 274

Query: 276 GSTILYADIYTPLVDMINNPQKYGFEQTNIGCCGTGLAEAGPLCNASTPTCEDPSKFMFW 335
           GS I Y DIY  L DM  NPQ+YG ++T  GCCGTG  E   LCNA T  C +P++++FW
Sbjct: 275 GSVIFYGDIYGALFDMATNPQRYGLKETTRGCCGTGEIELAYLCNALTRICPNPNQYLFW 334

Query: 336 DSIHPTEATYKFITESLLKQ 356
           D IHP++  Y  I+ SL++Q
Sbjct: 335 DDIHPSQIAYIVISLSLVEQ 354

BLAST of Bhi10G000663 vs. TAIR 10
Match: AT1G58430.1 (GDSL-like Lipase/Acylhydrolase family protein )

HSP 1 Score: 362.5 bits (929), Expect = 4.0e-100
Identity = 175/320 (54.69%), Postives = 232/320 (72.50%), Query Frame = 0

Query: 31  ETSPSFPAILIFGDSTVDTGNNNF-IPTIFKANYSPYGKDFPGHVATGRFSDGKLIPDMV 90
           +T P FPAILIFGDSTVDTGNNN+   TIF+A + PYG D P H   GRFS+GK+  D++
Sbjct: 28  KTQPLFPAILIFGDSTVDTGNNNYPSQTIFRAKHVPYGIDLPNHSPNGRFSNGKIFSDII 87

Query: 91  ASKLGIKELVPPFLDPELSDDDVKTGVSFASAGTGLDDRTAAISTVIPAMKQIGMFKNYI 150
           A+KL IK+ VPPFL P L+D ++ TGV FASAG G DD+T+  +  I   +Q  MFK+YI
Sbjct: 88  ATKLNIKQFVPPFLQPNLTDQEIVTGVCFASAGAGYDDQTSLTTQAIRVSEQPNMFKSYI 147

Query: 151 QRLQRIVGVDESKRIIGSALAVISVGTNDLTFNFYDLPT-RQLQFNISDYQDFLQNRLQS 210
            RL+ IVG  ++ +II +AL V+S G ND   N+Y++P+ R++  +ISDYQDF+ +RL +
Sbjct: 148 ARLKSIVGDKKAMKIINNALVVVSAGPNDFILNYYEVPSWRRMYPSISDYQDFVLSRLNN 207

Query: 211 LIKEIYQLGCRTIVVAGLPPIGCLPIQETISFPIPLNRKCLEDQNTDSEAYNQKLSKLLG 270
            +KE+Y LGCR I+V GLPP+GCLPIQ T  F   L R CLE +N DS  YNQKL KLL 
Sbjct: 208 FVKELYSLGCRKILVGGLPPMGCLPIQMTAQFRNVL-RFCLEQENRDSVLYNQKLQKLLP 267

Query: 271 NLQSQLLGSTILYADIYTPLVDMINNPQKYGFEQTNIGCCGTGLAEAGPLCNASTPTCED 330
             Q+ L GS ILY+D+Y P+++M+ NP KYGF++T  GCCGTG  E   +CNA +  C++
Sbjct: 268 QTQASLTGSKILYSDVYDPMMEMLQNPSKYGFKETTRGCCGTGFLETSFMCNAYSSMCQN 327

Query: 331 PSKFMFWDSIHPTEATYKFI 349
            S+F+F+DSIHP+EATY +I
Sbjct: 328 RSEFLFFDSIHPSEATYNYI 346

BLAST of Bhi10G000663 vs. ExPASy Swiss-Prot
Match: O22927 (GDSL esterase/lipase At2g30310 OS=Arabidopsis thaliana OX=3702 GN=At2g30310 PE=2 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 1.4e-102
Identity = 187/325 (57.54%), Postives = 228/325 (70.15%), Query Frame = 0

Query: 26  IALTPETSPSFPAILIFGDSTVDTGNNNF-IPTIFKANYSPYGKDFPGHVATGRFSDGKL 85
           +A    T P FPAILIFGDSTVDTGNNN+   TIFKA + PYG D PGH A GR+S+GK+
Sbjct: 22  VAANATTQPLFPAILIFGDSTVDTGNNNYHSQTIFKAKHLPYGVDLPGHEANGRYSNGKV 81

Query: 86  IPDMVASKLGIKELVPPFLDPELSDDDVKTGVSFASAGTGLDDRTAAISTVIPAMKQIGM 145
           I D++ASKL IKELVPPFL P +S  D+ TGVSFASAG G DDR++  S  IP  +Q  M
Sbjct: 82  ISDVIASKLNIKELVPPFLQPNISHQDIVTGVSFASAGAGYDDRSSLSSKAIPVSQQPSM 141

Query: 146 FKNYIQRLQRIVGVDESKRIIGSALAVISVGTNDLTFNFYDLPTRQLQF-NISDYQDFLQ 205
           FKNYI RL+ IVG  ++  II +AL VIS G ND   NFYD+PTR+L++  I  YQ+F+ 
Sbjct: 142 FKNYIARLKGIVGDKKAMEIINNALVVISAGPNDFILNFYDIPTRRLEYPTIHGYQEFIL 201

Query: 206 NRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETISFPIPLNRKCLEDQNTDSEAYNQKL 265
            RL   ++E+Y LGCR IVV GLPP+GCLPIQ T      L R C+E +N DS  YNQKL
Sbjct: 202 KRLDGFVRELYSLGCRNIVVGGLPPMGCLPIQMTAKMRNIL-RFCVEQENKDSVLYNQKL 261

Query: 266 SKLLGNLQSQLLGSTILYADIYTPLVDMINNPQKYGFEQTNIGCCGTGLAEAGPLCNAST 325
            K L  +Q+ L GS  LYA++Y PL+DMI NP KYGF++T  GCCGTG  E   +CN  T
Sbjct: 262 VKKLPEIQASLPGSNFLYANVYDPLMDMIQNPSKYGFKETKKGCCGTGYLETTFMCNPLT 321

Query: 326 PTCEDPSKFMFWDSIHPTEATYKFI 349
            TC + S  +FWDSIHP+EA Y +I
Sbjct: 322 KTCPNHSDHLFWDSIHPSEAAYNYI 345

BLAST of Bhi10G000663 vs. ExPASy Swiss-Prot
Match: Q9SJA9 (GDSL esterase/lipase At2g24560 OS=Arabidopsis thaliana OX=3702 GN=At2g24560 PE=2 SV=2)

HSP 1 Score: 370.9 bits (951), Expect = 1.6e-101
Identity = 187/346 (54.05%), Postives = 246/346 (71.10%), Query Frame = 0

Query: 9   VALSLHVTWLLFLS---KPCIALTPETS-PSFPAILIFGDSTVDTGNNNF-IPTIFKANY 68
           ++ S  +T+ LF++     C A T  TS P FPAILIFGDSTVDTGNNN+   TIFKA +
Sbjct: 1   MSTSKTITFTLFIAALLSSCDAATNATSQPLFPAILIFGDSTVDTGNNNYHSQTIFKAKH 60

Query: 69  SPYGKDFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDPELSDDDVKTGVSFASAGT 128
            PYG D P H A+GRF++GK+  D++A+KL IK+ VPPFL P LSD ++ TGV FASAG 
Sbjct: 61  LPYGIDLPNHKASGRFTNGKIFSDIIATKLNIKQFVPPFLQPNLSDQEIVTGVCFASAGA 120

Query: 129 GLDDRTAAISTVIPAMKQIGMFKNYIQRLQRIVGVDESKRIIGSALAVISVGTNDLTFNF 188
           G DD T+  +  I  + Q  MFKNYI RL+ IVG  ++  II +AL VIS G ND   N+
Sbjct: 121 GYDDHTSLSTQAIRVLDQQKMFKNYIARLKSIVGDKKAMEIIKNALVVISAGPNDFILNY 180

Query: 189 YDLPTRQLQF-NISDYQDFLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETISFPI 248
           YD+P+R+L+F +IS YQDF+  RL + ++E+Y LGCR I+V GLPP+GCLPIQ T  F  
Sbjct: 181 YDIPSRRLEFPHISGYQDFVLQRLDNFVRELYSLGCRKIMVGGLPPMGCLPIQMTAKFRN 240

Query: 249 PLNRKCLEDQNTDSEAYNQKLSKLLGNLQSQLLGSTILYADIYTPLVDMINNPQKYGFEQ 308
            L R CLE +N DS  YNQKL  LL  +++ L GS ILY+++Y P++DM+ NP KYGF++
Sbjct: 241 AL-RFCLEQENRDSVLYNQKLQNLLPQIEASLTGSKILYSNVYDPMMDMMQNPSKYGFKE 300

Query: 309 TNIGCCGTGLAEAGPLCNASTPTCEDPSKFMFWDSIHPTEATYKFI 349
           T  GCCGTG  E   +CNA +PTC + S+F+F+DSIHP+EATY ++
Sbjct: 301 TKRGCCGTGHLETSFMCNAFSPTCRNHSEFLFFDSIHPSEATYNYM 345

BLAST of Bhi10G000663 vs. ExPASy Swiss-Prot
Match: O22918 (GDSL esterase/lipase At2g30220 OS=Arabidopsis thaliana OX=3702 GN=At2g30220 PE=3 SV=1)

HSP 1 Score: 366.3 bits (939), Expect = 3.9e-100
Identity = 183/338 (54.14%), Postives = 228/338 (67.46%), Query Frame = 0

Query: 25  CIALTPETSPSFPAILIFGDSTVDTGNNNFI-PTIFKANYSPYGKDFPGHVATGRFSDGK 84
           C A    T P FPAILIFGDST DTGNNN+    +FKAN+ PYG D PGH A GRFS+GK
Sbjct: 20  CNADANTTQPLFPAILIFGDSTADTGNNNYYSQAVFKANHLPYGVDLPGHEANGRFSNGK 79

Query: 85  LIPDMVASKLGIKELVPPFLDPELSDDDVKTGVSFASAGTGLDDRTAAISTVIPAMKQIG 144
           LI D++++KL IKE VPPFL P +SD D+ TGV FASAG G DD T+  S  IP  +Q  
Sbjct: 80  LISDVISTKLNIKEFVPPFLQPNISDQDIVTGVCFASAGAGYDDETSLSSKAIPVSQQPS 139

Query: 145 MFKNYIQRLQRIVGVDESKRIIGSALAVISVGTNDLTFNFYDLPTRQLQF-NISDYQDFL 204
           MFKNYI RL+ IVG  ++  II +AL VIS G ND   NFYD+P R+L++  I  YQDF+
Sbjct: 140 MFKNYIARLKGIVGDKKAMEIINNALVVISAGPNDFILNFYDIPIRRLEYPTIYGYQDFV 199

Query: 205 QNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETISFPIPLNRKCLEDQNTDSEAYNQK 264
             RL   ++E+Y LGCR I+V GLPP+GCLPIQ T      L   C+E +N DS  YNQK
Sbjct: 200 LKRLDGFVRELYSLGCRNILVGGLPPMGCLPIQLTAKLRTILG-ICVEQENKDSILYNQK 259

Query: 265 LSKLLGNLQSQLLGSTILYADIYTPLVDMINNPQKYGFEQTNIGCCGTGLAEAGPLCNAS 324
           L K L  +Q+ L GS  LYA++Y P++DMI NP KYGF++T  GCCGTG  E   LC + 
Sbjct: 260 LVKKLPEIQASLPGSKFLYANVYDPVMDMIRNPSKYGFKETKKGCCGTGYLETSFLCTSL 319

Query: 325 TPTCEDPSKFMFWDSIHPTEATYKFITESLLKQFSDHL 361
           + TC + S  +FWDSIHP+EA YK++   +  Q  + L
Sbjct: 320 SKTCPNHSDHLFWDSIHPSEAAYKYLGNFIDAQIQEWL 356

BLAST of Bhi10G000663 vs. ExPASy Swiss-Prot
Match: Q9SIQ2 (GDSL esterase/lipase At2g31550 OS=Arabidopsis thaliana OX=3702 GN=At2g31550 PE=3 SV=3)

HSP 1 Score: 364.0 bits (933), Expect = 2.0e-99
Identity = 182/346 (52.60%), Postives = 237/346 (68.50%), Query Frame = 0

Query: 9   VALSLHVTWLLFLSKPCIALTPETSPSFPAILIFGDSTVDTGNNNF-IPTIFKANYSPYG 68
           + L+L +T  L  S    A    T P FPAILIFGDSTVDTGNNN+ +PTIF+A + PYG
Sbjct: 7   ITLTLFITTTLLASCDA-AANATTKPLFPAILIFGDSTVDTGNNNYPLPTIFRAEHFPYG 66

Query: 69  KDFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDPELSDDDVKTGVSFASAGTGLDD 128
            D P   A GRFS+GKLI D++A+KL IKE +PPFL P LSD D+ TGV FASAG G DD
Sbjct: 67  MDLPDGKANGRFSNGKLISDIIATKLNIKEFIPPFLQPNLSDQDILTGVCFASAGAGYDD 126

Query: 129 RTAAISTVIPAMKQIGMFKNYIQRLQRIVGVDESKRIIGSALAVISVGTNDLTFNFYDLP 188
            T+  +  I   +Q  MFK+YI RL+ IVG  ++  II +A  V+S G ND   N+YD+P
Sbjct: 127 LTSLSTQAIRVSEQPNMFKSYIARLKGIVGDKKAMEIINNAFVVVSAGPNDFILNYYDIP 186

Query: 189 TRQLQFN-ISDYQDFLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETISFPIPLNR 248
           +R+L++  IS YQDF+  RL++ ++E+Y LG R ++V GLPP+GCLPI  T  F   + R
Sbjct: 187 SRRLEYPFISGYQDFILKRLENFVRELYSLGVRNVLVGGLPPMGCLPIHMTAKFR-NIFR 246

Query: 249 KCLEDQNTDSEAYNQKLSKLLGNLQSQLLGSTILYADIYTPLVDMINNPQKYGFEQTNIG 308
            CLE  N DS  YN+KL KLL  +++ L GS  LYAD+Y P+++MI NP KYGF++T  G
Sbjct: 247 FCLEHHNKDSVLYNEKLQKLLPQIEASLPGSKFLYADVYNPMMEMIQNPSKYGFKETKRG 306

Query: 309 CCGTGLAEAGPLCNASTPTCEDPSKFMFWDSIHPTEATYKFITESL 353
           CCGTG  E   +CN  +P C++ S+FMF+DSIHP+EATY  I   L
Sbjct: 307 CCGTGFLETSFMCNVFSPVCQNRSEFMFFDSIHPSEATYNVIGNRL 350

BLAST of Bhi10G000663 vs. ExPASy Swiss-Prot
Match: Q9LMJ3 (GDSL esterase/lipase At1g06990 OS=Arabidopsis thaliana OX=3702 GN=At1g06990 PE=2 SV=2)

HSP 1 Score: 363.6 bits (932), Expect = 2.6e-99
Identity = 164/320 (51.25%), Postives = 227/320 (70.94%), Query Frame = 0

Query: 36  FPAILIFGDSTVDTGNNNFIPTIFKANYSPYGKDFPGHVATGRFSDGKLIPDMVASKLGI 95
           FPAIL+FGDST+DTGNNN+I T  +AN+ PYG +FPGH ATGRFS+GKLIPD +AS +GI
Sbjct: 35  FPAILVFGDSTIDTGNNNYIKTYIRANFPPYGCNFPGHNATGRFSNGKLIPDFIASLMGI 94

Query: 96  KELVPPFLDPELSDDDVKTGVSFASAGTGLDDRTAAISTVIPAMKQIGMFKNYIQRLQRI 155
           K+ VPPFLDP LSD D+ TGV FASAG+G D+ T   ++ +   KQ  M ++Y++RL +I
Sbjct: 95  KDTVPPFLDPHLSDSDIITGVCFASAGSGYDNLTDRATSTLSVDKQADMLRSYVERLSQI 154

Query: 156 VGVDESKRIIGSALAVISVGTNDLTFNFYDLPTRQLQFNISDYQDFLQNRLQSLIKEIYQ 215
           VG +++  I+  AL ++S GTND   N YD P+R+ +  +  YQ F+ + + + ++E+Y 
Sbjct: 155 VGDEKAASIVSEALVIVSSGTNDFNLNLYDTPSRRQKLGVDGYQSFILSNVHNFVQELYD 214

Query: 216 LGCRTIVVAGLPPIGCLPIQETISFPIPLNRKCLEDQNTDSEAYNQKLSKLLGNLQSQLL 275
           +GCR I+V GLPP+GCLPIQ T++      R+C++ QN+DS+ +NQKL   L  +QS L 
Sbjct: 215 IGCRKIMVLGLPPVGCLPIQMTMAMQKQNERRCIDKQNSDSQEFNQKLKNSLTEMQSNLT 274

Query: 276 GSTILYADIYTPLVDMINNPQKYGFEQTNIGCCGTGLAEAGPLCNASTPTCEDPSKFMFW 335
           GS I Y DIY  L DM  NPQ+YG ++T  GCCGTG  E   LCNA T  C +P++++FW
Sbjct: 275 GSVIFYGDIYGALFDMATNPQRYGLKETTRGCCGTGEIELAYLCNALTRICPNPNQYLFW 334

Query: 336 DSIHPTEATYKFITESLLKQ 356
           D IHP++  Y  I+ SL++Q
Sbjct: 335 DDIHPSQIAYIVISLSLVEQ 354

BLAST of Bhi10G000663 vs. NCBI nr
Match: XP_004143225.1 (GDSL esterase/lipase At2g30310 [Cucumis sativus])

HSP 1 Score: 656.4 bits (1692), Expect = 1.5e-184
Identity = 323/361 (89.47%), Postives = 338/361 (93.63%), Query Frame = 0

Query: 1   MARANYLIVALSLHVTWLLFLSKPCIALTPETSPSFPAILIFGDSTVDTGNNNFIPTIFK 60
           MARANYLI ALS+H+ WLLFLSKPC AL P+TS SF ++LIFGDSTVDTGNNNFIPTIFK
Sbjct: 1   MARANYLIAALSIHIIWLLFLSKPCSALAPKTSRSFSSVLIFGDSTVDTGNNNFIPTIFK 60

Query: 61  ANYSPYGKDFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDPELSDDDVKTGVSFAS 120
           ANY PYGKDFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDPELSDDDVKTGVSFAS
Sbjct: 61  ANYWPYGKDFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDPELSDDDVKTGVSFAS 120

Query: 121 AGTGLDDRTAAISTVIPAMKQIGMFKNYIQRLQRIVGVDESKRIIGSALAVISVGTNDLT 180
           AGTG+DD TAAIS VIPAMKQI MFKNYIQRLQRIVGVDESKRIIGSALAVISVGTNDLT
Sbjct: 121 AGTGVDDLTAAISKVIPAMKQIDMFKNYIQRLQRIVGVDESKRIIGSALAVISVGTNDLT 180

Query: 181 FNFYDLPTRQLQFNISDYQDFLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETISF 240
           FNFYD+PTRQLQ+NIS YQ+FLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETIS 
Sbjct: 181 FNFYDIPTRQLQYNISGYQEFLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETISS 240

Query: 241 PIPLNRKCLEDQNTDSEAYNQKLSKLLGNLQSQLLGSTILYADIYTPLVDMINNPQKYGF 300
           PIPLNR+CLE QN D+EAYNQKLSKLLG+LQ QL GS ILYADIYTPL+DMINNPQKYGF
Sbjct: 241 PIPLNRRCLEYQNKDAEAYNQKLSKLLGSLQPQLPGSQILYADIYTPLMDMINNPQKYGF 300

Query: 301 EQTNIGCCGTGLAEAGPLCNASTPTCEDPSKFMFWDSIHPTEATYKFITESLLKQFSDHL 360
           EQTNIGCCGTGL EAGPLCN  TPTCEDPSKFMFWDSIHP+EATYKF+TESLL QF D L
Sbjct: 301 EQTNIGCCGTGLVEAGPLCNKITPTCEDPSKFMFWDSIHPSEATYKFVTESLLNQFFDRL 360

Query: 361 N 362
           N
Sbjct: 361 N 361

BLAST of Bhi10G000663 vs. NCBI nr
Match: KAE8646939.1 (hypothetical protein Csa_021052 [Cucumis sativus])

HSP 1 Score: 652.9 bits (1683), Expect = 1.6e-183
Identity = 321/360 (89.17%), Postives = 337/360 (93.61%), Query Frame = 0

Query: 1   MARANYLIVALSLHVTWLLFLSKPCIALTPETSPSFPAILIFGDSTVDTGNNNFIPTIFK 60
           MARANYLI ALS+H+ WLLFLSKPC AL P+TS SF ++LIFGDSTVDTGNNNFIPTIFK
Sbjct: 1   MARANYLIAALSIHIIWLLFLSKPCSALAPKTSRSFSSVLIFGDSTVDTGNNNFIPTIFK 60

Query: 61  ANYSPYGKDFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDPELSDDDVKTGVSFAS 120
           ANY PYGKDFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDPELSDDDVKTGVSFAS
Sbjct: 61  ANYWPYGKDFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDPELSDDDVKTGVSFAS 120

Query: 121 AGTGLDDRTAAISTVIPAMKQIGMFKNYIQRLQRIVGVDESKRIIGSALAVISVGTNDLT 180
           AGTG+DD TAAIS VIPAMKQI MFKNYIQRLQRIVGVDESKRIIGSALAVISVGTNDLT
Sbjct: 121 AGTGVDDLTAAISKVIPAMKQIDMFKNYIQRLQRIVGVDESKRIIGSALAVISVGTNDLT 180

Query: 181 FNFYDLPTRQLQFNISDYQDFLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETISF 240
           FNFYD+PTRQLQ+NIS YQ+FLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETIS 
Sbjct: 181 FNFYDIPTRQLQYNISGYQEFLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETISS 240

Query: 241 PIPLNRKCLEDQNTDSEAYNQKLSKLLGNLQSQLLGSTILYADIYTPLVDMINNPQKYGF 300
           PIPLNR+CLE QN D+EAYNQKLSKLLG+LQ QL GS ILYADIYTPL+DMINNPQKYGF
Sbjct: 241 PIPLNRRCLEYQNKDAEAYNQKLSKLLGSLQPQLPGSQILYADIYTPLMDMINNPQKYGF 300

Query: 301 EQTNIGCCGTGLAEAGPLCNASTPTCEDPSKFMFWDSIHPTEATYKFITESLLKQFSDHL 360
           EQTNIGCCGTGL EAGPLCN  TPTCEDPSKFMFWDSIHP+EATYKF+TESLL QF D +
Sbjct: 301 EQTNIGCCGTGLVEAGPLCNKITPTCEDPSKFMFWDSIHPSEATYKFVTESLLNQFFDRV 360

BLAST of Bhi10G000663 vs. NCBI nr
Match: XP_008463828.1 (PREDICTED: GDSL esterase/lipase At2g30310-like [Cucumis melo] >KAA0059943.1 GDSL esterase/lipase [Cucumis melo var. makuwa] >TYK07588.1 GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 644.8 bits (1662), Expect = 4.4e-181
Identity = 317/361 (87.81%), Postives = 334/361 (92.52%), Query Frame = 0

Query: 1   MARANYLIVALSLHVTWLLFLSKPCIALTPETSPSFPAILIFGDSTVDTGNNNFIPTIFK 60
           MARANYLI ALS+H+ WLL LSKPC AL P+TS SFPAILIFGDSTVDTGNNNFI TIFK
Sbjct: 1   MARANYLITALSIHIIWLLLLSKPCSALAPKTSRSFPAILIFGDSTVDTGNNNFISTIFK 60

Query: 61  ANYSPYGKDFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDPELSDDDVKTGVSFAS 120
           ANY PYGKDFPG VATGRFSDGKLIPDMVASKLGIKELVPPFLDP+LSDDDVKTGVSFAS
Sbjct: 61  ANYWPYGKDFPGQVATGRFSDGKLIPDMVASKLGIKELVPPFLDPKLSDDDVKTGVSFAS 120

Query: 121 AGTGLDDRTAAISTVIPAMKQIGMFKNYIQRLQRIVGVDESKRIIGSALAVISVGTNDLT 180
           AGTG+DD TAAIS VIP MKQI MFKNYIQRLQRI GVDESKRIIGSAL VISVGTNDLT
Sbjct: 121 AGTGVDDLTAAISKVIPTMKQIDMFKNYIQRLQRIAGVDESKRIIGSALTVISVGTNDLT 180

Query: 181 FNFYDLPTRQLQFNISDYQDFLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETISF 240
           +NFYD+PTRQLQ+NIS YQ+FLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETIS 
Sbjct: 181 YNFYDIPTRQLQYNISGYQEFLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETISS 240

Query: 241 PIPLNRKCLEDQNTDSEAYNQKLSKLLGNLQSQLLGSTILYADIYTPLVDMINNPQKYGF 300
           PIPLNR+CLE QN D+EAYNQKLSKLLG+LQ QL GSTILYADIYTPL+DMINNP+KYGF
Sbjct: 241 PIPLNRRCLEYQNKDAEAYNQKLSKLLGSLQPQLPGSTILYADIYTPLMDMINNPKKYGF 300

Query: 301 EQTNIGCCGTGLAEAGPLCNASTPTCEDPSKFMFWDSIHPTEATYKFITESLLKQFSDHL 360
           EQTNIGCCGTGL EAGPLCN   PTCEDPSK+MFWDSIHP+EATYKF+TESLL QFSD L
Sbjct: 301 EQTNIGCCGTGLVEAGPLCNKVIPTCEDPSKYMFWDSIHPSEATYKFVTESLLNQFSDRL 360

Query: 361 N 362
           N
Sbjct: 361 N 361

BLAST of Bhi10G000663 vs. NCBI nr
Match: XP_022138897.1 (GDSL esterase/lipase At2g30310-like [Momordica charantia])

HSP 1 Score: 604.4 bits (1557), Expect = 6.6e-169
Identity = 295/360 (81.94%), Postives = 320/360 (88.89%), Query Frame = 0

Query: 1   MARANYLIVALSLHVTWLLFLSKPCIALTPETSPSFPAILIFGDSTVDTGNNNFIPTIFK 60
           MARANYLIVALSLH+   LFLSKP  AL P TSPSFPAILIFGDSTVDTGNNNFIPT+FK
Sbjct: 1   MARANYLIVALSLHMILQLFLSKPSSALAPTTSPSFPAILIFGDSTVDTGNNNFIPTVFK 60

Query: 61  ANYSPYGKDFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDPELSDDDVKTGVSFAS 120
           ANY PYGKDFP HVATGRFS+GKLIPDMVASKLGIK+LVPPFLDP+LSDD+VKTGVSFAS
Sbjct: 61  ANYFPYGKDFPDHVATGRFSNGKLIPDMVASKLGIKDLVPPFLDPKLSDDEVKTGVSFAS 120

Query: 121 AGTGLDDRTAAISTVIPAMKQIGMFKNYIQRLQRIVGVDESKRIIGSALAVISVGTNDLT 180
           AGTG DD TA +  VIP MKQI +FKNYIQRLQ IVGV+ESKRIIG AL VIS GTND  
Sbjct: 121 AGTGFDDLTAIVPKVIPVMKQIDLFKNYIQRLQGIVGVEESKRIIGRALVVISAGTNDFN 180

Query: 181 FNFYDLPTRQLQFNISDYQDFLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETISF 240
           FNFYDLPTR+LQF+I  YQDFLQNRLQ+LIKEIYQLGCR +VVAGLPPIGCLPIQETI+F
Sbjct: 181 FNFYDLPTRRLQFDIGGYQDFLQNRLQNLIKEIYQLGCRNMVVAGLPPIGCLPIQETIAF 240

Query: 241 PIPLNRKCLEDQNTDSEAYNQKLSKLLGNLQSQLLGSTILYADIYTPLVDMINNPQKYGF 300
             PL+RKCL+ QN DS+AYNQKLSKLLGNLQ QL GSTILYADIYTPL++MIN+P+ YGF
Sbjct: 241 ENPLDRKCLDGQNLDSQAYNQKLSKLLGNLQPQLAGSTILYADIYTPLINMINSPRNYGF 300

Query: 301 EQTNIGCCGTGLAEAGPLCNASTPTCEDPSKFMFWDSIHPTEATYKFITESLLKQFSDHL 360
           EQTNIGCCGTGL EAGPLCN  T TCE+PSKF+FWDSIHPTE TYKFI +SLL+Q  DHL
Sbjct: 301 EQTNIGCCGTGLVEAGPLCNPKTATCENPSKFLFWDSIHPTETTYKFIADSLLEQLGDHL 360

BLAST of Bhi10G000663 vs. NCBI nr
Match: XP_008463830.1 (PREDICTED: GDSL esterase/lipase At2g30310-like [Cucumis melo] >KAA0059942.1 GDSL esterase/lipase [Cucumis melo var. makuwa] >TYK07587.1 GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 599.0 bits (1543), Expect = 2.8e-167
Identity = 290/362 (80.11%), Postives = 324/362 (89.50%), Query Frame = 0

Query: 1   MARANYLIVALSLHVTWLLFLSKPCIALTPETSPSFPAILIFGDSTVDTGNNNFIPTIFK 60
           MARA Y I ALSLH+ WLLFLSKPC AL P+ + SFP+ILIFGDSTVDTGNNNFI TIFK
Sbjct: 1   MARATYHIAALSLHMIWLLFLSKPCTALEPKITRSFPSILIFGDSTVDTGNNNFISTIFK 60

Query: 61  ANYSPYGKDFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDPELSDDDVKTGVSFAS 120
           ANYSPYG DFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLD +LSDD+VKTGVSFAS
Sbjct: 61  ANYSPYGADFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDTKLSDDNVKTGVSFAS 120

Query: 121 AGTGLDDRTAAISTVIPAMKQIGMFKNYIQRLQRIVGVDESKRIIGSALAVISVGTNDLT 180
           AG+G D+ TA++S VIP MKQI MFKNYI+RLQ IVGVDES++I+ SAL +IS GTND+ 
Sbjct: 121 AGSGFDELTASVSNVIPVMKQIDMFKNYIRRLQGIVGVDESRKILNSALVIISAGTNDVN 180

Query: 181 FNFYDLPTRQLQFNISDYQDFLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETISF 240
            NFYDLPTRQLQ+NIS YQDFLQ+RLQSLIKEIYQLGCRT+VVAGLPP+GCLPIQE+I+F
Sbjct: 181 INFYDLPTRQLQYNISGYQDFLQDRLQSLIKEIYQLGCRTMVVAGLPPVGCLPIQESIAF 240

Query: 241 PIPLNRKCLEDQNTDSEAYNQKLSKLLGNLQSQLLGSTILYADIYTPLVDMINNPQKYGF 300
             PL+RKCLE+QN+DS+AYNQKL+ LL NLQ QL GSTILYADIYTPL+DM+NNP  YGF
Sbjct: 241 QNPLDRKCLEEQNSDSKAYNQKLAHLLSNLQPQLPGSTILYADIYTPLIDMVNNPHNYGF 300

Query: 301 EQTNIGCCGTGLAEAGPLCNASTPT-CEDPSKFMFWDSIHPTEATYKFITESLLKQFSDH 360
           E  N+GCCGTG+AEAGPLCN+ T T CE+PSKFMFWDSIHPTEA YKFITESLLKQF+D 
Sbjct: 301 EHVNVGCCGTGMAEAGPLCNSKTSTICENPSKFMFWDSIHPTEAAYKFITESLLKQFADR 360

Query: 361 LN 362
           LN
Sbjct: 361 LN 362

BLAST of Bhi10G000663 vs. ExPASy TrEMBL
Match: A0A0A0KF79 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G180980 PE=3 SV=1)

HSP 1 Score: 656.4 bits (1692), Expect = 7.1e-185
Identity = 323/361 (89.47%), Postives = 338/361 (93.63%), Query Frame = 0

Query: 1   MARANYLIVALSLHVTWLLFLSKPCIALTPETSPSFPAILIFGDSTVDTGNNNFIPTIFK 60
           MARANYLI ALS+H+ WLLFLSKPC AL P+TS SF ++LIFGDSTVDTGNNNFIPTIFK
Sbjct: 1   MARANYLIAALSIHIIWLLFLSKPCSALAPKTSRSFSSVLIFGDSTVDTGNNNFIPTIFK 60

Query: 61  ANYSPYGKDFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDPELSDDDVKTGVSFAS 120
           ANY PYGKDFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDPELSDDDVKTGVSFAS
Sbjct: 61  ANYWPYGKDFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDPELSDDDVKTGVSFAS 120

Query: 121 AGTGLDDRTAAISTVIPAMKQIGMFKNYIQRLQRIVGVDESKRIIGSALAVISVGTNDLT 180
           AGTG+DD TAAIS VIPAMKQI MFKNYIQRLQRIVGVDESKRIIGSALAVISVGTNDLT
Sbjct: 121 AGTGVDDLTAAISKVIPAMKQIDMFKNYIQRLQRIVGVDESKRIIGSALAVISVGTNDLT 180

Query: 181 FNFYDLPTRQLQFNISDYQDFLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETISF 240
           FNFYD+PTRQLQ+NIS YQ+FLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETIS 
Sbjct: 181 FNFYDIPTRQLQYNISGYQEFLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETISS 240

Query: 241 PIPLNRKCLEDQNTDSEAYNQKLSKLLGNLQSQLLGSTILYADIYTPLVDMINNPQKYGF 300
           PIPLNR+CLE QN D+EAYNQKLSKLLG+LQ QL GS ILYADIYTPL+DMINNPQKYGF
Sbjct: 241 PIPLNRRCLEYQNKDAEAYNQKLSKLLGSLQPQLPGSQILYADIYTPLMDMINNPQKYGF 300

Query: 301 EQTNIGCCGTGLAEAGPLCNASTPTCEDPSKFMFWDSIHPTEATYKFITESLLKQFSDHL 360
           EQTNIGCCGTGL EAGPLCN  TPTCEDPSKFMFWDSIHP+EATYKF+TESLL QF D L
Sbjct: 301 EQTNIGCCGTGLVEAGPLCNKITPTCEDPSKFMFWDSIHPSEATYKFVTESLLNQFFDRL 360

Query: 361 N 362
           N
Sbjct: 361 N 361

BLAST of Bhi10G000663 vs. ExPASy TrEMBL
Match: A0A5D3C8T5 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold852G00280 PE=3 SV=1)

HSP 1 Score: 644.8 bits (1662), Expect = 2.1e-181
Identity = 317/361 (87.81%), Postives = 334/361 (92.52%), Query Frame = 0

Query: 1   MARANYLIVALSLHVTWLLFLSKPCIALTPETSPSFPAILIFGDSTVDTGNNNFIPTIFK 60
           MARANYLI ALS+H+ WLL LSKPC AL P+TS SFPAILIFGDSTVDTGNNNFI TIFK
Sbjct: 1   MARANYLITALSIHIIWLLLLSKPCSALAPKTSRSFPAILIFGDSTVDTGNNNFISTIFK 60

Query: 61  ANYSPYGKDFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDPELSDDDVKTGVSFAS 120
           ANY PYGKDFPG VATGRFSDGKLIPDMVASKLGIKELVPPFLDP+LSDDDVKTGVSFAS
Sbjct: 61  ANYWPYGKDFPGQVATGRFSDGKLIPDMVASKLGIKELVPPFLDPKLSDDDVKTGVSFAS 120

Query: 121 AGTGLDDRTAAISTVIPAMKQIGMFKNYIQRLQRIVGVDESKRIIGSALAVISVGTNDLT 180
           AGTG+DD TAAIS VIP MKQI MFKNYIQRLQRI GVDESKRIIGSAL VISVGTNDLT
Sbjct: 121 AGTGVDDLTAAISKVIPTMKQIDMFKNYIQRLQRIAGVDESKRIIGSALTVISVGTNDLT 180

Query: 181 FNFYDLPTRQLQFNISDYQDFLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETISF 240
           +NFYD+PTRQLQ+NIS YQ+FLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETIS 
Sbjct: 181 YNFYDIPTRQLQYNISGYQEFLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETISS 240

Query: 241 PIPLNRKCLEDQNTDSEAYNQKLSKLLGNLQSQLLGSTILYADIYTPLVDMINNPQKYGF 300
           PIPLNR+CLE QN D+EAYNQKLSKLLG+LQ QL GSTILYADIYTPL+DMINNP+KYGF
Sbjct: 241 PIPLNRRCLEYQNKDAEAYNQKLSKLLGSLQPQLPGSTILYADIYTPLMDMINNPKKYGF 300

Query: 301 EQTNIGCCGTGLAEAGPLCNASTPTCEDPSKFMFWDSIHPTEATYKFITESLLKQFSDHL 360
           EQTNIGCCGTGL EAGPLCN   PTCEDPSK+MFWDSIHP+EATYKF+TESLL QFSD L
Sbjct: 301 EQTNIGCCGTGLVEAGPLCNKVIPTCEDPSKYMFWDSIHPSEATYKFVTESLLNQFSDRL 360

Query: 361 N 362
           N
Sbjct: 361 N 361

BLAST of Bhi10G000663 vs. ExPASy TrEMBL
Match: A0A1S3CK41 (GDSL esterase/lipase At2g30310-like OS=Cucumis melo OX=3656 GN=LOC103501877 PE=3 SV=1)

HSP 1 Score: 644.8 bits (1662), Expect = 2.1e-181
Identity = 317/361 (87.81%), Postives = 334/361 (92.52%), Query Frame = 0

Query: 1   MARANYLIVALSLHVTWLLFLSKPCIALTPETSPSFPAILIFGDSTVDTGNNNFIPTIFK 60
           MARANYLI ALS+H+ WLL LSKPC AL P+TS SFPAILIFGDSTVDTGNNNFI TIFK
Sbjct: 1   MARANYLITALSIHIIWLLLLSKPCSALAPKTSRSFPAILIFGDSTVDTGNNNFISTIFK 60

Query: 61  ANYSPYGKDFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDPELSDDDVKTGVSFAS 120
           ANY PYGKDFPG VATGRFSDGKLIPDMVASKLGIKELVPPFLDP+LSDDDVKTGVSFAS
Sbjct: 61  ANYWPYGKDFPGQVATGRFSDGKLIPDMVASKLGIKELVPPFLDPKLSDDDVKTGVSFAS 120

Query: 121 AGTGLDDRTAAISTVIPAMKQIGMFKNYIQRLQRIVGVDESKRIIGSALAVISVGTNDLT 180
           AGTG+DD TAAIS VIP MKQI MFKNYIQRLQRI GVDESKRIIGSAL VISVGTNDLT
Sbjct: 121 AGTGVDDLTAAISKVIPTMKQIDMFKNYIQRLQRIAGVDESKRIIGSALTVISVGTNDLT 180

Query: 181 FNFYDLPTRQLQFNISDYQDFLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETISF 240
           +NFYD+PTRQLQ+NIS YQ+FLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETIS 
Sbjct: 181 YNFYDIPTRQLQYNISGYQEFLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETISS 240

Query: 241 PIPLNRKCLEDQNTDSEAYNQKLSKLLGNLQSQLLGSTILYADIYTPLVDMINNPQKYGF 300
           PIPLNR+CLE QN D+EAYNQKLSKLLG+LQ QL GSTILYADIYTPL+DMINNP+KYGF
Sbjct: 241 PIPLNRRCLEYQNKDAEAYNQKLSKLLGSLQPQLPGSTILYADIYTPLMDMINNPKKYGF 300

Query: 301 EQTNIGCCGTGLAEAGPLCNASTPTCEDPSKFMFWDSIHPTEATYKFITESLLKQFSDHL 360
           EQTNIGCCGTGL EAGPLCN   PTCEDPSK+MFWDSIHP+EATYKF+TESLL QFSD L
Sbjct: 301 EQTNIGCCGTGLVEAGPLCNKVIPTCEDPSKYMFWDSIHPSEATYKFVTESLLNQFSDRL 360

Query: 361 N 362
           N
Sbjct: 361 N 361

BLAST of Bhi10G000663 vs. ExPASy TrEMBL
Match: A0A6J1CCH7 (GDSL esterase/lipase At2g30310-like OS=Momordica charantia OX=3673 GN=LOC111009962 PE=3 SV=1)

HSP 1 Score: 604.4 bits (1557), Expect = 3.2e-169
Identity = 295/360 (81.94%), Postives = 320/360 (88.89%), Query Frame = 0

Query: 1   MARANYLIVALSLHVTWLLFLSKPCIALTPETSPSFPAILIFGDSTVDTGNNNFIPTIFK 60
           MARANYLIVALSLH+   LFLSKP  AL P TSPSFPAILIFGDSTVDTGNNNFIPT+FK
Sbjct: 1   MARANYLIVALSLHMILQLFLSKPSSALAPTTSPSFPAILIFGDSTVDTGNNNFIPTVFK 60

Query: 61  ANYSPYGKDFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDPELSDDDVKTGVSFAS 120
           ANY PYGKDFP HVATGRFS+GKLIPDMVASKLGIK+LVPPFLDP+LSDD+VKTGVSFAS
Sbjct: 61  ANYFPYGKDFPDHVATGRFSNGKLIPDMVASKLGIKDLVPPFLDPKLSDDEVKTGVSFAS 120

Query: 121 AGTGLDDRTAAISTVIPAMKQIGMFKNYIQRLQRIVGVDESKRIIGSALAVISVGTNDLT 180
           AGTG DD TA +  VIP MKQI +FKNYIQRLQ IVGV+ESKRIIG AL VIS GTND  
Sbjct: 121 AGTGFDDLTAIVPKVIPVMKQIDLFKNYIQRLQGIVGVEESKRIIGRALVVISAGTNDFN 180

Query: 181 FNFYDLPTRQLQFNISDYQDFLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETISF 240
           FNFYDLPTR+LQF+I  YQDFLQNRLQ+LIKEIYQLGCR +VVAGLPPIGCLPIQETI+F
Sbjct: 181 FNFYDLPTRRLQFDIGGYQDFLQNRLQNLIKEIYQLGCRNMVVAGLPPIGCLPIQETIAF 240

Query: 241 PIPLNRKCLEDQNTDSEAYNQKLSKLLGNLQSQLLGSTILYADIYTPLVDMINNPQKYGF 300
             PL+RKCL+ QN DS+AYNQKLSKLLGNLQ QL GSTILYADIYTPL++MIN+P+ YGF
Sbjct: 241 ENPLDRKCLDGQNLDSQAYNQKLSKLLGNLQPQLAGSTILYADIYTPLINMINSPRNYGF 300

Query: 301 EQTNIGCCGTGLAEAGPLCNASTPTCEDPSKFMFWDSIHPTEATYKFITESLLKQFSDHL 360
           EQTNIGCCGTGL EAGPLCN  T TCE+PSKF+FWDSIHPTE TYKFI +SLL+Q  DHL
Sbjct: 301 EQTNIGCCGTGLVEAGPLCNPKTATCENPSKFLFWDSIHPTETTYKFIADSLLEQLGDHL 360

BLAST of Bhi10G000663 vs. ExPASy TrEMBL
Match: A0A5A7UVN0 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold852G00270 PE=3 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 1.3e-167
Identity = 290/362 (80.11%), Postives = 324/362 (89.50%), Query Frame = 0

Query: 1   MARANYLIVALSLHVTWLLFLSKPCIALTPETSPSFPAILIFGDSTVDTGNNNFIPTIFK 60
           MARA Y I ALSLH+ WLLFLSKPC AL P+ + SFP+ILIFGDSTVDTGNNNFI TIFK
Sbjct: 1   MARATYHIAALSLHMIWLLFLSKPCTALEPKITRSFPSILIFGDSTVDTGNNNFISTIFK 60

Query: 61  ANYSPYGKDFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDPELSDDDVKTGVSFAS 120
           ANYSPYG DFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLD +LSDD+VKTGVSFAS
Sbjct: 61  ANYSPYGADFPGHVATGRFSDGKLIPDMVASKLGIKELVPPFLDTKLSDDNVKTGVSFAS 120

Query: 121 AGTGLDDRTAAISTVIPAMKQIGMFKNYIQRLQRIVGVDESKRIIGSALAVISVGTNDLT 180
           AG+G D+ TA++S VIP MKQI MFKNYI+RLQ IVGVDES++I+ SAL +IS GTND+ 
Sbjct: 121 AGSGFDELTASVSNVIPVMKQIDMFKNYIRRLQGIVGVDESRKILNSALVIISAGTNDVN 180

Query: 181 FNFYDLPTRQLQFNISDYQDFLQNRLQSLIKEIYQLGCRTIVVAGLPPIGCLPIQETISF 240
            NFYDLPTRQLQ+NIS YQDFLQ+RLQSLIKEIYQLGCRT+VVAGLPP+GCLPIQE+I+F
Sbjct: 181 INFYDLPTRQLQYNISGYQDFLQDRLQSLIKEIYQLGCRTMVVAGLPPVGCLPIQESIAF 240

Query: 241 PIPLNRKCLEDQNTDSEAYNQKLSKLLGNLQSQLLGSTILYADIYTPLVDMINNPQKYGF 300
             PL+RKCLE+QN+DS+AYNQKL+ LL NLQ QL GSTILYADIYTPL+DM+NNP  YGF
Sbjct: 241 QNPLDRKCLEEQNSDSKAYNQKLAHLLSNLQPQLPGSTILYADIYTPLIDMVNNPHNYGF 300

Query: 301 EQTNIGCCGTGLAEAGPLCNASTPT-CEDPSKFMFWDSIHPTEATYKFITESLLKQFSDH 360
           E  N+GCCGTG+AEAGPLCN+ T T CE+PSKFMFWDSIHPTEA YKFITESLLKQF+D 
Sbjct: 301 EHVNVGCCGTGMAEAGPLCNSKTSTICENPSKFMFWDSIHPTEAAYKFITESLLKQFADR 360

Query: 361 LN 362
           LN
Sbjct: 361 LN 362

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT2G30310.11.0e-10357.54GDSL-like Lipase/Acylhydrolase family protein [more]
AT2G24560.11.1e-10254.05GDSL-like Lipase/Acylhydrolase family protein [more]
AT2G30220.12.8e-10154.14GDSL-like Lipase/Acylhydrolase family protein [more]
AT1G06990.11.8e-10051.25GDSL-like Lipase/Acylhydrolase superfamily protein [more]
AT1G58430.14.0e-10054.69GDSL-like Lipase/Acylhydrolase family protein [more]
Match NameE-valueIdentityDescription
O229271.4e-10257.54GDSL esterase/lipase At2g30310 OS=Arabidopsis thaliana OX=3702 GN=At2g30310 PE=2... [more]
Q9SJA91.6e-10154.05GDSL esterase/lipase At2g24560 OS=Arabidopsis thaliana OX=3702 GN=At2g24560 PE=2... [more]
O229183.9e-10054.14GDSL esterase/lipase At2g30220 OS=Arabidopsis thaliana OX=3702 GN=At2g30220 PE=3... [more]
Q9SIQ22.0e-9952.60GDSL esterase/lipase At2g31550 OS=Arabidopsis thaliana OX=3702 GN=At2g31550 PE=3... [more]
Q9LMJ32.6e-9951.25GDSL esterase/lipase At1g06990 OS=Arabidopsis thaliana OX=3702 GN=At1g06990 PE=2... [more]
Match NameE-valueIdentityDescription
XP_004143225.11.5e-18489.47GDSL esterase/lipase At2g30310 [Cucumis sativus][more]
KAE8646939.11.6e-18389.17hypothetical protein Csa_021052 [Cucumis sativus][more]
XP_008463828.14.4e-18187.81PREDICTED: GDSL esterase/lipase At2g30310-like [Cucumis melo] >KAA0059943.1 GDSL... [more]
XP_022138897.16.6e-16981.94GDSL esterase/lipase At2g30310-like [Momordica charantia][more]
XP_008463830.12.8e-16780.11PREDICTED: GDSL esterase/lipase At2g30310-like [Cucumis melo] >KAA0059942.1 GDSL... [more]
Match NameE-valueIdentityDescription
A0A0A0KF797.1e-18589.47Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G180980 PE=3 SV=1[more]
A0A5D3C8T52.1e-18187.81GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold852... [more]
A0A1S3CK412.1e-18187.81GDSL esterase/lipase At2g30310-like OS=Cucumis melo OX=3656 GN=LOC103501877 PE=3... [more]
A0A6J1CCH73.2e-16981.94GDSL esterase/lipase At2g30310-like OS=Momordica charantia OX=3673 GN=LOC1110099... [more]
A0A5A7UVN01.3e-16780.11GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold852... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001087GDSL lipase/esterasePFAMPF00657Lipase_GDSLcoord: 39..351
e-value: 1.0E-35
score: 124.2
IPR036514SGNH hydrolase superfamilyGENE3D3.40.50.1110SGNH hydrolasecoord: 28..359
e-value: 1.1E-76
score: 260.4
NoneNo IPR availablePANTHERPTHR45642:SF30GDSL-LIKE LIPASE/ACYLHYDROLASEcoord: 33..351
NoneNo IPR availablePANTHERPTHR45642GDSL ESTERASE/LIPASE EXL3coord: 33..351
NoneNo IPR availableSUPERFAMILY52266SGNH hydrolasecoord: 37..358
IPR035669GDSL lipase/esterase-like, plantCDDcd01837SGNH_plant_lipase_likecoord: 37..353
e-value: 1.19579E-145
score: 412.779

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi10M000663Bhi10M000663mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016788 hydrolase activity, acting on ester bonds