Bhi10G000535 (gene) Wax gourd (B227) v1

Overview
NameBhi10G000535
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationchr10: 14545824 .. 14546444 (-)
RNA-Seq ExpressionBhi10G000535
SyntenyBhi10G000535
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCATCGAGTCCGCCGCCGCCATCTCCGGTGCCTGATTACTCTAGCTGGAGTCCGTGGTCAATCTCGACCATCGCCATCGTCTGCATAGTCTTTCTACTGTTGAGCAACTATAGACTTTTGAAACATCTGTTCCGTGTGTTTCATGGACTGATTTCCGGTCGACCTAGGGTCCAATTTGAGGAAAACCCTAATGGTCCTTCTCTGCAAGTCCATGGACATGGATTGGAGGCCTCCATGATTCGTTTGCTACCTATATCACAGTTCAAAAAGAATGAAGAATCCGAATCAACGGCAAGCTTCAATACGGAATGCGCTGTTTGTTTGGGTGAATTCGAAGAAGGGGAATTGATTAAACACTTACCGAATTGCAATCATTCGTTTCATAGTTGTTGCATCGACGCCTGGTTTCGTGATCATTCGAGTTGCCCTCTCTGCAGATTGCAAGTCTTGAGTTTCTCCACTCGCGATTCTTCTTCGGCAATGATGGAGACGCTTAGAGGAGAAAACGCCCTACGAGAAAGAGTAGCGCATTATGAAACGATTCGTGCTCAAATTCTGCAGCAGCCGGAATTGAGAAGAGATGCATCGGTTCAAGGATCAGATCAACGAAATCGATGA

mRNA sequence

ATGCCATCGAGTCCGCCGCCGCCATCTCCGGTGCCTGATTACTCTAGCTGGAGTCCGTGGTCAATCTCGACCATCGCCATCGTCTGCATAGTCTTTCTACTGTTGAGCAACTATAGACTTTTGAAACATCTGTTCCGTGTGTTTCATGGACTGATTTCCGGTCGACCTAGGGTCCAATTTGAGGAAAACCCTAATGGTCCTTCTCTGCAAGTCCATGGACATGGATTGGAGGCCTCCATGATTCGTTTGCTACCTATATCACAGTTCAAAAAGAATGAAGAATCCGAATCAACGGCAAGCTTCAATACGGAATGCGCTGTTTGTTTGGGTGAATTCGAAGAAGGGGAATTGATTAAACACTTACCGAATTGCAATCATTCGTTTCATAGTTGTTGCATCGACGCCTGGTTTCGTGATCATTCGAGTTGCCCTCTCTGCAGATTGCAAGTCTTGAGTTTCTCCACTCGCGATTCTTCTTCGGCAATGATGGAGACGCTTAGAGGAGAAAACGCCCTACGAGAAAGAGTAGCGCATTATGAAACGATTCGTGCTCAAATTCTGCAGCAGCCGGAATTGAGAAGAGATGCATCGGTTCAAGGATCAGATCAACGAAATCGATGA

Coding sequence (CDS)

ATGCCATCGAGTCCGCCGCCGCCATCTCCGGTGCCTGATTACTCTAGCTGGAGTCCGTGGTCAATCTCGACCATCGCCATCGTCTGCATAGTCTTTCTACTGTTGAGCAACTATAGACTTTTGAAACATCTGTTCCGTGTGTTTCATGGACTGATTTCCGGTCGACCTAGGGTCCAATTTGAGGAAAACCCTAATGGTCCTTCTCTGCAAGTCCATGGACATGGATTGGAGGCCTCCATGATTCGTTTGCTACCTATATCACAGTTCAAAAAGAATGAAGAATCCGAATCAACGGCAAGCTTCAATACGGAATGCGCTGTTTGTTTGGGTGAATTCGAAGAAGGGGAATTGATTAAACACTTACCGAATTGCAATCATTCGTTTCATAGTTGTTGCATCGACGCCTGGTTTCGTGATCATTCGAGTTGCCCTCTCTGCAGATTGCAAGTCTTGAGTTTCTCCACTCGCGATTCTTCTTCGGCAATGATGGAGACGCTTAGAGGAGAAAACGCCCTACGAGAAAGAGTAGCGCATTATGAAACGATTCGTGCTCAAATTCTGCAGCAGCCGGAATTGAGAAGAGATGCATCGGTTCAAGGATCAGATCAACGAAATCGATGA

Protein sequence

MPSSPPPPSPVPDYSSWSPWSISTIAIVCIVFLLLSNYRLLKHLFRVFHGLISGRPRVQFEENPNGPSLQVHGHGLEASMIRLLPISQFKKNEESESTASFNTECAVCLGEFEEGELIKHLPNCNHSFHSCCIDAWFRDHSSCPLCRLQVLSFSTRDSSSAMMETLRGENALRERVAHYETIRAQILQQPELRRDASVQGSDQRNR
Homology
BLAST of Bhi10G000535 vs. TAIR 10
Match: AT5G17600.1 (RING/U-box superfamily protein )

HSP 1 Score: 92.0 bits (227), Expect = 5.8e-19
Identity = 59/167 (35.33%), Postives = 85/167 (50.90%), Query Frame = 0

Query: 5   PPPPSPVPD------YSSWSPWSISTIAIVCIVFLLLSNYRLL-KHLFRVFHGLISGRPR 64
           PPPPS   D       SS+SP  I+ I I+    +L+S Y L+ K+  R  H   S    
Sbjct: 36  PPPPSFFLDDDSSSSSSSFSPLLIALIGILTSALILVSYYTLISKYCHR--HHQTSSSET 95

Query: 65  VQFEENPNG-----PSLQVHGHGLEASMIRLLPISQFKKNEESESTASFNTECAVCLGEF 124
           +    N  G       +  +G GL  SMI+ + + ++K  +         ++C+VCL EF
Sbjct: 96  LNLNHNGEGFFSSTQRISTNGDGLNESMIKSITVYKYKSGDGFVD----GSDCSVCLSEF 155

Query: 125 EEGELIKHLPNCNHSFHSCCIDAWFRDHSSCPLCRLQVLSFSTRDSS 160
           EE E ++ LP CNH+FH  CID W + HS+CPLCR  V   +   +S
Sbjct: 156 EENESLRLLPKCNHAFHLPCIDTWLKSHSNCPLCRAFVTGVNNPTAS 196

BLAST of Bhi10G000535 vs. TAIR 10
Match: AT5G43420.1 (RING/U-box superfamily protein )

HSP 1 Score: 88.6 bits (218), Expect = 6.4e-18
Identity = 65/195 (33.33%), Postives = 100/195 (51.28%), Query Frame = 0

Query: 5   PPPPSPV-----PDYSSWSPWSISTIAIVCIVFLLLSNYRLLKHLFRVFHGL-ISGRPRV 64
           PPPP P+        +S+   +++ I I+   FLL+S Y  +      +H + I GR  +
Sbjct: 17  PPPPPPIFHRASSTGTSFPILAVAVIGILATAFLLVSYYVFVIKCCLNWHRIDILGRFSL 76

Query: 65  -QFEENPNGP----SLQVHGHGLEASMIRLLPISQFKK----------NEESESTASFNT 124
            +   N   P    S ++   GL+ S+IR +PI +FKK           E  E     + 
Sbjct: 77  SRRRRNDQDPLMVYSPELRSRGLDESVIRAIPIFKFKKRYDQNDGVFTGEGEEEEEKRSQ 136

Query: 125 ECAVCLGEFEEGELIKHLPNCNHSFHSCCIDAWFRDHSSCPLCRLQV---LSF-----ST 171
           EC+VCL EF++ E ++ +PNC+H FH  CID W +++++CPLCR +V    SF     S 
Sbjct: 137 ECSVCLSEFQDEEKLRIIPNCSHLFHIDCIDVWLQNNANCPLCRTRVSCDTSFPPDRVSA 196

BLAST of Bhi10G000535 vs. TAIR 10
Match: AT3G16720.1 (TOXICOS EN LEVADURA 2 )

HSP 1 Score: 87.8 bits (216), Expect = 1.1e-17
Identity = 44/94 (46.81%), Postives = 57/94 (60.64%), Query Frame = 0

Query: 71  VHGHGLEASMIRLLPISQFKKNEESESTASFNTECAVCLGEFEEGELIKHLPNCNHSFHS 130
           V   GL+ ++I+ LP+  F     S+ T     ECAVCL EFEE E  + LPNC H+FH 
Sbjct: 90  VASRGLDPNVIKSLPVFTF-----SDETHKDPIECAVCLSEFEESETGRVLPNCQHTFHV 149

Query: 131 CCIDAWFRDHSSCPLCRLQVLSFSTRDSSSAMME 165
            CID WF  HS+CPLCR  V S +  +S++A  E
Sbjct: 150 DCIDMWFHSHSTCPLCRSLVESLAGIESTAAARE 178

BLAST of Bhi10G000535 vs. TAIR 10
Match: AT1G04360.1 (RING/U-box superfamily protein )

HSP 1 Score: 86.7 bits (213), Expect = 2.4e-17
Identity = 57/168 (33.93%), Postives = 85/168 (50.60%), Query Frame = 0

Query: 6   PPPSPVPDYSSWSP--------------WSISTIAIVCIVFLLLSNYRLLKHLFRVFHGL 65
           PPP P P  +  SP               +I+ I I+   FLL+S Y  +      +H +
Sbjct: 16  PPPPPPPSTTFTSPIFPRTSSSGTNFPILAIAVIGILATAFLLVSYYIFVIKCCLNWHQI 75

Query: 66  -ISGRPRVQFEENPNGPSLQVH-----GHGLEASMIRLLPISQFKKNE---ESESTASFN 125
            I  R R   ++NP    L ++       GL+ S IR +P+ +FKK +     E  +  +
Sbjct: 76  DIFRRRRRSSDQNP----LMIYSPHEVNRGLDESAIRAIPVFKFKKRDVVAGEEDQSKNS 135

Query: 126 TECAVCLGEFEEGELIKHLPNCNHSFHSCCIDAWFRDHSSCPLCRLQV 151
            EC+VCL EF+E E ++ +PNC H FH  CID W + +++CPLCR  V
Sbjct: 136 QECSVCLNEFQEDEKLRIIPNCCHVFHIDCIDIWLQGNANCPLCRTSV 179

BLAST of Bhi10G000535 vs. TAIR 10
Match: AT3G03550.1 (RING/U-box superfamily protein )

HSP 1 Score: 85.5 bits (210), Expect = 5.5e-17
Identity = 58/175 (33.14%), Postives = 89/175 (50.86%), Query Frame = 0

Query: 5   PPPPSPVPD------YSSWSPWSISTIAIVCIVFLLLSNYRLL-KHLFRVFHGLISGRP- 64
           PPPPS   D       S +SP  I+ I I+   F+L+S Y L+ K+  R  H   S    
Sbjct: 38  PPPPSFYLDDEDDSSSSDFSPLLIALIGILASAFILVSYYTLISKYCHRRRHNSSSTSAA 97

Query: 65  ---RVQFE--------------ENPNGPSLQVHGHGLEASMIRLLPISQFKKNEESESTA 124
              R+  +               NPN       G GL+ S+I+ + + +++K +      
Sbjct: 98  AINRISSDYTWQGTNNNNNNGATNPNQTIGGGGGDGLDESLIKSITVYKYRKMDGFVE-- 157

Query: 125 SFNTECAVCLGEFEEGELIKHLPNCNHSFHSCCIDAWFRDHSSCPLCRLQVLSFS 155
             +++C+VCL EF+E E ++ LP CNH+FH  CID W + HS+CPLCR  +++ S
Sbjct: 158 --SSDCSVCLSEFQENESLRLLPKCNHAFHVPCIDTWLKSHSNCPLCRAFIVTSS 208

BLAST of Bhi10G000535 vs. ExPASy Swiss-Prot
Match: Q9LF64 (RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 8.2e-18
Identity = 59/167 (35.33%), Postives = 85/167 (50.90%), Query Frame = 0

Query: 5   PPPPSPVPD------YSSWSPWSISTIAIVCIVFLLLSNYRLL-KHLFRVFHGLISGRPR 64
           PPPPS   D       SS+SP  I+ I I+    +L+S Y L+ K+  R  H   S    
Sbjct: 36  PPPPSFFLDDDSSSSSSSFSPLLIALIGILTSALILVSYYTLISKYCHR--HHQTSSSET 95

Query: 65  VQFEENPNG-----PSLQVHGHGLEASMIRLLPISQFKKNEESESTASFNTECAVCLGEF 124
           +    N  G       +  +G GL  SMI+ + + ++K  +         ++C+VCL EF
Sbjct: 96  LNLNHNGEGFFSSTQRISTNGDGLNESMIKSITVYKYKSGDGFVD----GSDCSVCLSEF 155

Query: 125 EEGELIKHLPNCNHSFHSCCIDAWFRDHSSCPLCRLQVLSFSTRDSS 160
           EE E ++ LP CNH+FH  CID W + HS+CPLCR  V   +   +S
Sbjct: 156 EENESLRLLPKCNHAFHLPCIDTWLKSHSNCPLCRAFVTGVNNPTAS 196

BLAST of Bhi10G000535 vs. ExPASy Swiss-Prot
Match: Q9LSW9 (RING-H2 finger protein ATL16 OS=Arabidopsis thaliana OX=3702 GN=ATL16 PE=2 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 9.1e-17
Identity = 65/195 (33.33%), Postives = 100/195 (51.28%), Query Frame = 0

Query: 5   PPPPSPV-----PDYSSWSPWSISTIAIVCIVFLLLSNYRLLKHLFRVFHGL-ISGRPRV 64
           PPPP P+        +S+   +++ I I+   FLL+S Y  +      +H + I GR  +
Sbjct: 17  PPPPPPIFHRASSTGTSFPILAVAVIGILATAFLLVSYYVFVIKCCLNWHRIDILGRFSL 76

Query: 65  -QFEENPNGP----SLQVHGHGLEASMIRLLPISQFKK----------NEESESTASFNT 124
            +   N   P    S ++   GL+ S+IR +PI +FKK           E  E     + 
Sbjct: 77  SRRRRNDQDPLMVYSPELRSRGLDESVIRAIPIFKFKKRYDQNDGVFTGEGEEEEEKRSQ 136

Query: 125 ECAVCLGEFEEGELIKHLPNCNHSFHSCCIDAWFRDHSSCPLCRLQV---LSF-----ST 171
           EC+VCL EF++ E ++ +PNC+H FH  CID W +++++CPLCR +V    SF     S 
Sbjct: 137 ECSVCLSEFQDEEKLRIIPNCSHLFHIDCIDVWLQNNANCPLCRTRVSCDTSFPPDRVSA 196

BLAST of Bhi10G000535 vs. ExPASy Swiss-Prot
Match: Q8L9T5 (RING-H2 finger protein ATL2 OS=Arabidopsis thaliana OX=3702 GN=ATL2 PE=2 SV=2)

HSP 1 Score: 87.8 bits (216), Expect = 1.5e-16
Identity = 44/94 (46.81%), Postives = 57/94 (60.64%), Query Frame = 0

Query: 71  VHGHGLEASMIRLLPISQFKKNEESESTASFNTECAVCLGEFEEGELIKHLPNCNHSFHS 130
           V   GL+ ++I+ LP+  F     S+ T     ECAVCL EFEE E  + LPNC H+FH 
Sbjct: 90  VASRGLDPNVIKSLPVFTF-----SDETHKDPIECAVCLSEFEESETGRVLPNCQHTFHV 149

Query: 131 CCIDAWFRDHSSCPLCRLQVLSFSTRDSSSAMME 165
            CID WF  HS+CPLCR  V S +  +S++A  E
Sbjct: 150 DCIDMWFHSHSTCPLCRSLVESLAGIESTAAARE 178

BLAST of Bhi10G000535 vs. ExPASy Swiss-Prot
Match: P93823 (RING-H2 finger protein ATL1 OS=Arabidopsis thaliana OX=3702 GN=ATL1 PE=2 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 3.4e-16
Identity = 57/168 (33.93%), Postives = 85/168 (50.60%), Query Frame = 0

Query: 6   PPPSPVPDYSSWSP--------------WSISTIAIVCIVFLLLSNYRLLKHLFRVFHGL 65
           PPP P P  +  SP               +I+ I I+   FLL+S Y  +      +H +
Sbjct: 16  PPPPPPPSTTFTSPIFPRTSSSGTNFPILAIAVIGILATAFLLVSYYIFVIKCCLNWHQI 75

Query: 66  -ISGRPRVQFEENPNGPSLQVH-----GHGLEASMIRLLPISQFKKNE---ESESTASFN 125
            I  R R   ++NP    L ++       GL+ S IR +P+ +FKK +     E  +  +
Sbjct: 76  DIFRRRRRSSDQNP----LMIYSPHEVNRGLDESAIRAIPVFKFKKRDVVAGEEDQSKNS 135

Query: 126 TECAVCLGEFEEGELIKHLPNCNHSFHSCCIDAWFRDHSSCPLCRLQV 151
            EC+VCL EF+E E ++ +PNC H FH  CID W + +++CPLCR  V
Sbjct: 136 QECSVCLNEFQEDEKLRIIPNCCHVFHIDCIDIWLQGNANCPLCRTSV 179

BLAST of Bhi10G000535 vs. ExPASy Swiss-Prot
Match: Q8LF65 (RING-H2 finger protein ATL17 OS=Arabidopsis thaliana OX=3702 GN=ATL17 PE=2 SV=2)

HSP 1 Score: 86.3 bits (212), Expect = 4.5e-16
Identity = 43/86 (50.00%), Postives = 53/86 (61.63%), Query Frame = 0

Query: 75  GLEASMIRLLPISQFKKNEESESTASFNTECAVCLGEFEEGELIKHLPNCNHSFHSCCID 134
           GL  S+I+ LPI  F     S  TA F  EC+VCL EF++ E  + +PNC H+FH  CID
Sbjct: 51  GLNPSIIKSLPIFTF-----SAVTALFAMECSVCLSEFKDNESGRVMPNCKHTFHVHCID 110

Query: 135 AWFRDHSSCPLCRLQVLSFSTRDSSS 161
            WF  HSSCPLCR Q+  F+    SS
Sbjct: 111 MWFHSHSSCPLCRSQIEPFAGGVKSS 131

BLAST of Bhi10G000535 vs. NCBI nr
Match: XP_031743630.1 (RING-H2 finger protein ATL52-like [Cucumis sativus] >KGN47355.1 hypothetical protein Csa_023057 [Cucumis sativus])

HSP 1 Score: 366.7 bits (940), Expect = 1.3e-97
Identity = 181/207 (87.44%), Postives = 190/207 (91.79%), Query Frame = 0

Query: 1   MPSSPPPPSPVPDYSSWSPWSISTIAIVCIVFLLLSNYRLLKHLFRVFHGLISGRPRVQF 60
           MPSSPPPPSPVPDYSSWSPWSISTI +VCIVFLLLSNYRLLK L RV HGL +GRP VQF
Sbjct: 1   MPSSPPPPSPVPDYSSWSPWSISTIVVVCIVFLLLSNYRLLKQLCRVLHGLFTGRPTVQF 60

Query: 61  EENPNGPSLQVHGHGLEASMIRLLPISQFKKNEESESTASFNTECAVCLGEFEEGELIKH 120
           EENPN PSLQ+HGHGLE SMIRLLPISQFKKNEESEST SFNTECAVCLGEFEEGELIKH
Sbjct: 61  EENPNNPSLQIHGHGLETSMIRLLPISQFKKNEESESTTSFNTECAVCLGEFEEGELIKH 120

Query: 121 LPNCNHSFHSCCIDAWFRDHSSCPLCRLQVLSFSTRD-SSSAMMETLRGENALRERVAHY 180
           LPNCNHSFHS CIDAWFR+HSSCPLCRLQVLSFST D SSSAM+ETL  EN LRERVAHY
Sbjct: 121 LPNCNHSFHSPCIDAWFRNHSSCPLCRLQVLSFSTPDSSSSAMLETLGRENVLRERVAHY 180

Query: 181 ETIRAQILQQPELRRDASVQGSDQRNR 207
           +T+RAQILQ PE RRDAS+QG+DQRNR
Sbjct: 181 QTLRAQILQHPEFRRDASIQGTDQRNR 207

BLAST of Bhi10G000535 vs. NCBI nr
Match: XP_008448367.1 (PREDICTED: RING-H2 finger protein ATL66-like [Cucumis melo] >KAA0061954.1 RING-H2 finger protein ATL66-like [Cucumis melo var. makuwa] >TYK23976.1 RING-H2 finger protein ATL66-like [Cucumis melo var. makuwa])

HSP 1 Score: 357.5 bits (916), Expect = 8.0e-95
Identity = 175/205 (85.37%), Postives = 184/205 (89.76%), Query Frame = 0

Query: 1   MPSSPPPPSPVPDYSSWSPWSISTIAIVCIVFLLLSNYRLLKHLFRVFHGLISGRPRVQF 60
           MPSSPPPPSPVPDYSSWSPWSISTI IVC++FLLLSNYRLLK L RV H L SGRP VQF
Sbjct: 1   MPSSPPPPSPVPDYSSWSPWSISTIVIVCVIFLLLSNYRLLKQLCRVLHELFSGRPAVQF 60

Query: 61  EENPNGPSLQVHGHGLEASMIRLLPISQFKKNEESESTASFNTECAVCLGEFEEGELIKH 120
           EENPN PSLQ+H HGLE SMIRLLPISQFKK EESEST SFNTECAVCLGEFEEGELIK+
Sbjct: 61  EENPNNPSLQIHAHGLETSMIRLLPISQFKKKEESESTTSFNTECAVCLGEFEEGELIKN 120

Query: 121 LPNCNHSFHSCCIDAWFRDHSSCPLCRLQVLSFSTRDSSSAMMETLRGENALRERVAHYE 180
           LPNCNHSFHS CIDAWFRDHSSCPLCRLQV SFST DSSSAM+ETL  EN LRERVAHY+
Sbjct: 121 LPNCNHSFHSSCIDAWFRDHSSCPLCRLQVWSFSTPDSSSAMLETLGRENILRERVAHYQ 180

Query: 181 TIRAQILQQPELRRDASVQGSDQRN 206
           T+RAQILQ PE RRD S+QGSDQR+
Sbjct: 181 TLRAQILQHPEFRRDESIQGSDQRD 205

BLAST of Bhi10G000535 vs. NCBI nr
Match: XP_023512261.1 (RING-H2 finger protein ATL74-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 320.9 bits (821), Expect = 8.3e-84
Identity = 167/213 (78.40%), Postives = 180/213 (84.51%), Query Frame = 0

Query: 1   MPSS---PPPPSPVPDYSSWSPWSISTIAIVCIVFLLLSNYRLLKHLFRVFHGLISGRPR 60
           MPSS   PPPPSPVPDYSSWSPWSIS IAIVCIV LLLSNYR+LK   RVFHGL  GR  
Sbjct: 1   MPSSPPPPPPPSPVPDYSSWSPWSISIIAIVCIVVLLLSNYRILKRWCRVFHGLFCGRSV 60

Query: 61  VQFEENPNGPSLQVHGHGLEASMIRLLPISQFKKNEESESTASFNTECAVCLGEFEEGEL 120
           VQF+ENPN PS QVH HGLEAS IRLLPISQFKKNEE+ESTAS NTECAVCLGEFEEGEL
Sbjct: 61  VQFDENPNDPSPQVHVHGLEASTIRLLPISQFKKNEEAESTASINTECAVCLGEFEEGEL 120

Query: 121 IKHLPNCNHSFHSCCIDAWFRDHSSCPLCRLQVLSFS----TRDSSSAMMETLRGENALR 180
           IK+LPNCNHSFHS CIDAW R+HSSCPLCRL V + S    T DSSSAM+ETL  E+   
Sbjct: 121 IKNLPNCNHSFHSSCIDAWLRNHSSCPLCRLHVSNLSIDCPTPDSSSAMLETLSREDVDG 180

Query: 181 ERVAHYETIRAQILQQPELRRDASVQGSDQRNR 207
           ERV++Y+T+RAQILQ P+LRRDASVQGSDQRNR
Sbjct: 181 ERVSYYQTLRAQILQNPDLRRDASVQGSDQRNR 213

BLAST of Bhi10G000535 vs. NCBI nr
Match: KAG6570731.1 (RING-H2 finger protein ATL16, partial [Cucurbita argyrosperma subsp. sororia] >KAG7010575.1 RING-H2 finger protein ATL16, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 320.1 bits (819), Expect = 1.4e-83
Identity = 167/213 (78.40%), Postives = 180/213 (84.51%), Query Frame = 0

Query: 1   MPSS---PPPPSPVPDYSSWSPWSISTIAIVCIVFLLLSNYRLLKHLFRVFHGLISGRPR 60
           MPSS   PPPPSPVPDYSSWSPWSIS IAIVCIV LLLSNYR+LK   RV HGL  GR  
Sbjct: 1   MPSSPPPPPPPSPVPDYSSWSPWSISIIAIVCIVVLLLSNYRILKRWCRVLHGLFCGRFV 60

Query: 61  VQFEENPNGPSLQVHGHGLEASMIRLLPISQFKKNEESESTASFNTECAVCLGEFEEGEL 120
           VQF+ENPN PS +VH HGLEAS IRLLPISQFKKNEE+ESTAS NTECAVCLGEFEEGEL
Sbjct: 61  VQFDENPNDPSPEVHVHGLEASTIRLLPISQFKKNEEAESTASINTECAVCLGEFEEGEL 120

Query: 121 IKHLPNCNHSFHSCCIDAWFRDHSSCPLCRLQVLSFS----TRDSSSAMMETLRGENALR 180
           IK+LPNCNHSFHS CIDAW R+HSSCPLCRL V + S    T DSSSAM+ETL  E+  R
Sbjct: 121 IKNLPNCNHSFHSSCIDAWLRNHSSCPLCRLHVSNLSIDCPTPDSSSAMLETLGREDVER 180

Query: 181 ERVAHYETIRAQILQQPELRRDASVQGSDQRNR 207
           ERV++Y+T+RAQILQ PELRRDASVQGSDQRNR
Sbjct: 181 ERVSYYQTLRAQILQNPELRRDASVQGSDQRNR 213

BLAST of Bhi10G000535 vs. NCBI nr
Match: KAF3453818.1 (hypothetical protein FNV43_RR04259 [Rhamnella rubrinervis])

HSP 1 Score: 169.1 bits (427), Expect = 4.0e-38
Identity = 88/196 (44.90%), Postives = 124/196 (63.27%), Query Frame = 0

Query: 9   SPVPDYSSWSPWSISTIAIVCIVFLLLSNYRLLKHLFRVFHGLISGRPRVQFEENPNGPS 68
           SP+P  S W+PW+I+++A V I+ LLLS +R+L+     F         +  E N + PS
Sbjct: 305 SPLPVPSHWNPWAIASVATVSIIILLLSYFRILQSFCCNFSSRNQAGSLLLSETNLDDPS 364

Query: 69  LQVHGHGLEASMIRLLPISQFK-KNEESESTASFNTECAVCLGEFEEGELIKHLPNCNHS 128
           LQ H HGLE S+I  LPI+QFK +N  S+     +T+CAVCLGEF+EGE ++ LPNC H+
Sbjct: 365 LQFHSHGLEVSLIHSLPITQFKEENGGSKMGHQTSTDCAVCLGEFQEGEWLRFLPNCKHA 424

Query: 129 FHSCCIDAWFRDHSSCPLCRLQVLSFSTRD-----SSSAMMETLRGENALRERVAHYETI 188
           FH  CID WF  HS+CPLCR +V  F+  D     S   ++ETLR E+  +ER AHY+ +
Sbjct: 425 FHISCIDTWFLSHSTCPLCRSEVNGFAFEDHEYPVSMYTLLETLRREDFFQERAAHYQFL 484

Query: 189 RAQILQQPELRRDASV 199
           ++ IL     R ++S+
Sbjct: 485 QSTILNNSVARTESSL 500

BLAST of Bhi10G000535 vs. ExPASy TrEMBL
Match: A0A0A0KCA5 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G303210 PE=3 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 6.4e-98
Identity = 181/207 (87.44%), Postives = 190/207 (91.79%), Query Frame = 0

Query: 1   MPSSPPPPSPVPDYSSWSPWSISTIAIVCIVFLLLSNYRLLKHLFRVFHGLISGRPRVQF 60
           MPSSPPPPSPVPDYSSWSPWSISTI +VCIVFLLLSNYRLLK L RV HGL +GRP VQF
Sbjct: 1   MPSSPPPPSPVPDYSSWSPWSISTIVVVCIVFLLLSNYRLLKQLCRVLHGLFTGRPTVQF 60

Query: 61  EENPNGPSLQVHGHGLEASMIRLLPISQFKKNEESESTASFNTECAVCLGEFEEGELIKH 120
           EENPN PSLQ+HGHGLE SMIRLLPISQFKKNEESEST SFNTECAVCLGEFEEGELIKH
Sbjct: 61  EENPNNPSLQIHGHGLETSMIRLLPISQFKKNEESESTTSFNTECAVCLGEFEEGELIKH 120

Query: 121 LPNCNHSFHSCCIDAWFRDHSSCPLCRLQVLSFSTRD-SSSAMMETLRGENALRERVAHY 180
           LPNCNHSFHS CIDAWFR+HSSCPLCRLQVLSFST D SSSAM+ETL  EN LRERVAHY
Sbjct: 121 LPNCNHSFHSPCIDAWFRNHSSCPLCRLQVLSFSTPDSSSSAMLETLGRENVLRERVAHY 180

Query: 181 ETIRAQILQQPELRRDASVQGSDQRNR 207
           +T+RAQILQ PE RRDAS+QG+DQRNR
Sbjct: 181 QTLRAQILQHPEFRRDASIQGTDQRNR 207

BLAST of Bhi10G000535 vs. ExPASy TrEMBL
Match: A0A5A7V3S2 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold250G00390 PE=3 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 3.9e-95
Identity = 175/205 (85.37%), Postives = 184/205 (89.76%), Query Frame = 0

Query: 1   MPSSPPPPSPVPDYSSWSPWSISTIAIVCIVFLLLSNYRLLKHLFRVFHGLISGRPRVQF 60
           MPSSPPPPSPVPDYSSWSPWSISTI IVC++FLLLSNYRLLK L RV H L SGRP VQF
Sbjct: 1   MPSSPPPPSPVPDYSSWSPWSISTIVIVCVIFLLLSNYRLLKQLCRVLHELFSGRPAVQF 60

Query: 61  EENPNGPSLQVHGHGLEASMIRLLPISQFKKNEESESTASFNTECAVCLGEFEEGELIKH 120
           EENPN PSLQ+H HGLE SMIRLLPISQFKK EESEST SFNTECAVCLGEFEEGELIK+
Sbjct: 61  EENPNNPSLQIHAHGLETSMIRLLPISQFKKKEESESTTSFNTECAVCLGEFEEGELIKN 120

Query: 121 LPNCNHSFHSCCIDAWFRDHSSCPLCRLQVLSFSTRDSSSAMMETLRGENALRERVAHYE 180
           LPNCNHSFHS CIDAWFRDHSSCPLCRLQV SFST DSSSAM+ETL  EN LRERVAHY+
Sbjct: 121 LPNCNHSFHSSCIDAWFRDHSSCPLCRLQVWSFSTPDSSSAMLETLGRENILRERVAHYQ 180

Query: 181 TIRAQILQQPELRRDASVQGSDQRN 206
           T+RAQILQ PE RRD S+QGSDQR+
Sbjct: 181 TLRAQILQHPEFRRDESIQGSDQRD 205

BLAST of Bhi10G000535 vs. ExPASy TrEMBL
Match: A0A1S3BIX6 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103490579 PE=3 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 3.9e-95
Identity = 175/205 (85.37%), Postives = 184/205 (89.76%), Query Frame = 0

Query: 1   MPSSPPPPSPVPDYSSWSPWSISTIAIVCIVFLLLSNYRLLKHLFRVFHGLISGRPRVQF 60
           MPSSPPPPSPVPDYSSWSPWSISTI IVC++FLLLSNYRLLK L RV H L SGRP VQF
Sbjct: 1   MPSSPPPPSPVPDYSSWSPWSISTIVIVCVIFLLLSNYRLLKQLCRVLHELFSGRPAVQF 60

Query: 61  EENPNGPSLQVHGHGLEASMIRLLPISQFKKNEESESTASFNTECAVCLGEFEEGELIKH 120
           EENPN PSLQ+H HGLE SMIRLLPISQFKK EESEST SFNTECAVCLGEFEEGELIK+
Sbjct: 61  EENPNNPSLQIHAHGLETSMIRLLPISQFKKKEESESTTSFNTECAVCLGEFEEGELIKN 120

Query: 121 LPNCNHSFHSCCIDAWFRDHSSCPLCRLQVLSFSTRDSSSAMMETLRGENALRERVAHYE 180
           LPNCNHSFHS CIDAWFRDHSSCPLCRLQV SFST DSSSAM+ETL  EN LRERVAHY+
Sbjct: 121 LPNCNHSFHSSCIDAWFRDHSSCPLCRLQVWSFSTPDSSSAMLETLGRENILRERVAHYQ 180

Query: 181 TIRAQILQQPELRRDASVQGSDQRN 206
           T+RAQILQ PE RRD S+QGSDQR+
Sbjct: 181 TLRAQILQHPEFRRDESIQGSDQRD 205

BLAST of Bhi10G000535 vs. ExPASy TrEMBL
Match: A0A2C9W1B6 (RING-type E3 ubiquitin transferase OS=Manihot esculenta OX=3983 GN=MANES_04G030200 PE=3 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 8.2e-37
Identity = 91/196 (46.43%), Postives = 120/196 (61.22%), Query Frame = 0

Query: 1   MPSSPPPPSP--VPDYSSWSPWSISTIAIVCIVFLLLSNYRLLKHLFRVFHGLISGRPRV 60
           MP SP P +P  + +YS W+ + I  +  VC   +L S YR+L+ L    +     R R 
Sbjct: 1   MPPSPTPEAPLRLENYSQWNAYVIGPVIAVCSSVVLFSYYRMLRRLCCTLNTQTLPRNRA 60

Query: 61  QF----EENPNGPSLQVHGHGLEASMIRLLPISQFKKNEESESTASFNTECAVCLGEFEE 120
           Q     + N    SLQ + HGLE++++R LPISQFKK EESE++   N ECAVCLGEFEE
Sbjct: 61  QMSRINQNNLEDSSLQNYSHGLESNIMRSLPISQFKKEEESEAS---NRECAVCLGEFEE 120

Query: 121 GELIKHLPNCNHSFHSCCIDAWFRDHSSCPLCRLQV--LSFSTRDSSSAMMETLRGENAL 180
           GE +KHLPNC H FH  CID WF+ HS+CPLCR  V  L      S   ++ TLR E+  
Sbjct: 121 GEWLKHLPNCAHVFHVACIDTWFQTHSNCPLCRSHVYDLCHEYSISMDTLLHTLRREDFF 180

Query: 181 RERVAHYETIRAQILQ 189
            ER  HY+ +R++IL+
Sbjct: 181 NERAEHYQNLRSEILR 193

BLAST of Bhi10G000535 vs. ExPASy TrEMBL
Match: A0A6A6KKG1 (RING-type E3 ubiquitin transferase OS=Hevea brasiliensis OX=3981 GN=GH714_013318 PE=3 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 1.8e-36
Identity = 94/198 (47.47%), Postives = 120/198 (60.61%), Query Frame = 0

Query: 1   MPSSPPPPS--PVPDYSSWSPWSISTIAIVCIVFLLLSNYRLLKHLFRVFHGLISGRPRV 60
           MP SP P +   + +   W+P+ I  +  VC   ++ S YR+LK L    + L   R R 
Sbjct: 1   MPLSPTPAAQHQLENSPEWNPYVIGPVVAVCTFIVMFSYYRILKRLCCSLNTLTFSRNRF 60

Query: 61  QF----EENPNGPSLQVHGHGLEASMIRLLPISQFKKNEESESTASFNTECAVCLGEFEE 120
           Q     E +    SLQ   HGLE++++  LPISQFKK +E ES  S N+ECAVCLGEFEE
Sbjct: 61  QMRRIGENSLGDSSLQYFSHGLESAIMCSLPISQFKKEKEEESRTS-NSECAVCLGEFEE 120

Query: 121 GELIKHLPNCNHSFHSCCIDAWFRDHSSCPLCRLQV--LSFSTRDSSSAMMETLRGENAL 180
           GE +KHLP C H FH  CID WF+ HS+CPLCR QV  LS     S S ++ETLR E+ L
Sbjct: 121 GEWLKHLPICAHVFHVACIDTWFQTHSNCPLCRSQVYDLSHEYSISVSILLETLRREDFL 180

Query: 181 RERVAHYETIRAQILQQP 191
            ER  HY+ +R +IL  P
Sbjct: 181 HERAKHYQILRPEILGNP 197

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G17600.15.8e-1935.33RING/U-box superfamily protein [more]
AT5G43420.16.4e-1833.33RING/U-box superfamily protein [more]
AT3G16720.11.1e-1746.81TOXICOS EN LEVADURA 2 [more]
AT1G04360.12.4e-1733.93RING/U-box superfamily protein [more]
AT3G03550.15.5e-1733.14RING/U-box superfamily protein [more]
Match NameE-valueIdentityDescription
Q9LF648.2e-1835.33RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1[more]
Q9LSW99.1e-1733.33RING-H2 finger protein ATL16 OS=Arabidopsis thaliana OX=3702 GN=ATL16 PE=2 SV=1[more]
Q8L9T51.5e-1646.81RING-H2 finger protein ATL2 OS=Arabidopsis thaliana OX=3702 GN=ATL2 PE=2 SV=2[more]
P938233.4e-1633.93RING-H2 finger protein ATL1 OS=Arabidopsis thaliana OX=3702 GN=ATL1 PE=2 SV=1[more]
Q8LF654.5e-1650.00RING-H2 finger protein ATL17 OS=Arabidopsis thaliana OX=3702 GN=ATL17 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
XP_031743630.11.3e-9787.44RING-H2 finger protein ATL52-like [Cucumis sativus] >KGN47355.1 hypothetical pro... [more]
XP_008448367.18.0e-9585.37PREDICTED: RING-H2 finger protein ATL66-like [Cucumis melo] >KAA0061954.1 RING-H... [more]
XP_023512261.18.3e-8478.40RING-H2 finger protein ATL74-like [Cucurbita pepo subsp. pepo][more]
KAG6570731.11.4e-8378.40RING-H2 finger protein ATL16, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
KAF3453818.14.0e-3844.90hypothetical protein FNV43_RR04259 [Rhamnella rubrinervis][more]
Match NameE-valueIdentityDescription
A0A0A0KCA56.4e-9887.44RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G303210 PE... [more]
A0A5A7V3S23.9e-9585.37RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3BIX63.9e-9585.37RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103490579 PE=3 ... [more]
A0A2C9W1B68.2e-3746.43RING-type E3 ubiquitin transferase OS=Manihot esculenta OX=3983 GN=MANES_04G0302... [more]
A0A6A6KKG11.8e-3647.47RING-type E3 ubiquitin transferase OS=Hevea brasiliensis OX=3981 GN=GH714_013318... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 105..146
e-value: 3.6E-5
score: 33.2
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 104..147
e-value: 2.3E-11
score: 43.8
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 105..147
score: 12.149959
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 67..151
e-value: 3.9E-20
score: 73.3
IPR044675E3 ubiquitin-protein ligase RING1-likePANTHERPTHR45676RING-H2 FINGER PROTEIN ATL51-RELATEDcoord: 14..159
NoneNo IPR availablePANTHERPTHR45676:SF107RING/U-BOX SUPERFAMILY PROTEINcoord: 14..159
NoneNo IPR availableCDDcd16454RING-H2_PA-TM-RINGcoord: 104..147
e-value: 3.59208E-16
score: 67.3269
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 104..153

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi10M000535Bhi10M000535mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0016021 integral component of membrane