Bhi10G000334 (gene) Wax gourd (B227) v1

Overview
NameBhi10G000334
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Descriptiontransmembrane protein 45A
Locationchr10: 9261919 .. 9267589 (+)
RNA-Seq ExpressionBhi10G000334
SyntenyBhi10G000334
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAACAACCTCGCCAACTCAATTCGAACCCTCTAGACCCTTTGAAGTTTGACCCTTCTCTTATAAGTTAACATTACATAAACTTACAAGTTACAATCCCCTTCATAACTCTACCTCCCTTCTTCTCTTTTTTCTTTGTTATTATTATGGGGACTTTAGTGGGTCATGTAGCGCCCGGGTTTGGGTTCTTCATCATTGGCCTATGGCATCTCTTCAACAATACCAAAATCGAAGCTCGTAACAACGATCCTCCAAATACCGTTAAATTCCTCTCATGGTTCCCCACTTCAAGATTCAAGTACTTAGAGCCGTGTCTGATCATGGTTGGCGCCTCGGCCTCCATAGCCATGGAGTTATTCATCGGCCCACAACAGCACCAGCCGTTCGACACGGACGGCACCATCCCTTCCTACCATCTCCACAACTTCGAACACTCCAACATTTCCATGACTTTCTTTGTGTTTGCTGCCTTTTGCGTTGTTTTGGATCGGCTTCGTCCGAAAGCTTATTTTGAGCTGACACAGTTTGTTGGGGGTGTGGCTTTTGGGCAAGAGCTTCTCTTGTTTCACCTTCACTCGGCTGACCATATGGGCCCTGAAGGACAATACCACTTGCTTCTTCAGCTTCTTGTGTTTGTGTCTTTTGCCACAACTCTCCTTGGGATTGCTTTTCCCACTAGCTTCTTGGTGGCGTTTGTGAGATCTCTTAGCATTGTTTTTCAAGGCCTTTGGCTTATGCTCATGGGCTTCGCGCTTTGGACTCCTTCTTTCATTCCTAAAGGTATGGTAACCAAAAAATTATTCACAGATATTTCCAGAAAATTTTTGTCTAAATATTTTTCAAAAATTTATGACGGCGGAAAATAATTTGACAATTATTGGTTCTCTCTTTTATAAAAAGTGGAAAATTAAGTTTGTAGCTAGTTTGTTTCTATCTTCTTTTCGTCTCATCTACAAGTAATTTAACGAAGGGGTAATAAGACAAGAGGTATTTTTCACAAACTTTTATTTTATATTAAATCATCAACCAATTTAAAAGTTTAAATGAATTGATTTCTTATCACCATAAATGACCAAAATAAATATTTAATGATCTTGAAATCTATAAACCAAATTGAAACAAATTTCAAATTTAAAAATTAAAATTTTGATATTGAATTAAAACTAAACTCAAGACTTAAGAATTAAATGTAACATTTTAAAATCTAACTAGATATTAAATAAAAACTACGCCCCGAATTTAAGGCAAAAATGCATTTTCCCTTATTTTTTAAAAGAATGGGAAAAAAACATTCTTTTGGTAGTTTTCTATCCTCATTTCTTGTTTGCCAAGACACACAATGGTTGGTAAATAATTAATACCTATCCGTTTACGTGGATTTGTTGAAATCTGTCGTTGTAGTAGTTTTTTTTTTTTTTAAATGATTATTTTTATATTTTTCTTTTTTATATATATATAAAAAAAATGAGTATGAAATTAGTTGCAGATTAGATTGCATTTGTCCAAATAATAACATTGATAAAGAAATTATAGTATGGTATATTAAAATAATGGATTTGATATATAATTGTATAATATATAGGTTGCTTTATGCACTTGGAAGATGGGCACAAGGTGGTGCGTTGCGCCGGGGAGGAGCCACTTCAGCGAGCCAAGGCGCTGATCAACCTTCAGTTCAGCTGGCTGTTAATTGCACTCACCATTTTCGCTTTATCATTTCATTTGCTTCTGCTTAAACTATACCAACAAGAAACGCCCAAGAACGCCGCCCACTACTTTTCATTAATGACCGACGACGAAGAAAAAGCATTAGAAGCAGCTGCCTCAACAAAACTAACACCATACGACGTCGCTTCCAGGAGTTTCATTCAAATGGGCCACAAGTTCACGGAAACGGACATGGAAAGGTAGAAACAAACTTCAAACCAAATGGATAAGCCTTAAATTTAAAATTATTGAGTGTGTAATCTGTATATATGGTTTGTTTGATTATTATTATTATTCATTGTGTTTGGGTGGGCTCCACACCTTTCATTTTCATTTTGTGTTAAATTTTAAGCCAAGCTTAAAATTCATGTGAAAAGCAGGCTTAGCTTAGCTCAAAACAAACACCTTTGCTCCATAATCAAAATTGTTACCATCATAATAAACTCTTTTATTATTCATAATTTATAAAATATTATTAATATATTTAAATGGATTCCACTTTTTATATTATAATGGATTGATTATATTTATTGAAAAATAAACGTGGCCTAATTTTGAAAAATACTTAAGTGCTTCCCAAATAATATTTATTTTTGTACATCCCATCAAATTGTCCAATTATAACTTGTCATGTGACAAATTTCTTAAAATATTTTGATTCTTTTTTTTATGTGAGTGATGAAAGGGGATGTATACATGAAGATCGGTACATCTTGACATACACCAAAATATTACTTCTATTTTCGTCCATGATCAATACATTTGTGGATTGAGGTAAATTTTAGATCAATATACCACAATTTAATCTGTTAGGTTATGATTAATCAGTTTATCTTTTTCGTGTTGATTTCTTTTTCTTCCACGAAGATTTGAATCTTTTGCAAATATTATTTTAATAAATTTTGGGGTTGCCCTCATGTTTCTGCAATACATAGAAAAACAAAATAAAAAAAACTATATAATTTTTCATAATGTTAAAATTGTTGAGAATATTTTCAAATATAACAAAATATCACTGTATATCAATGATAGACCGTGATAGACATCTATCAGTGTCAGTGATGGATATCTACGGTTAGCGTTGGCAACGGGGCAGGAGAGGGGGATGTATTCCCCATCCCCATCCTGGTGGAGATTTGTAATCTTCATCCTCACCCTATTTTCCATTTTGGGAAGTCGGAGTTGGGGTCGAGGAATCCCCATTCGGGGGTCGGGTCCCGCGGAGAAAAATTTTCGTTTATATTTTATTTTGAATTATTTATTTGTTTAAAACTAATTAAAATTTGATATTTTATATTGAAATTATAAGGGAGCGTTTGGGGCAAAAACTATCCTAAGACTATTGTAACCATATATTACTACAGTAAATACTATTTTAAAACCCCCAATTAACTATAGTCAACACTATTTCAAAACACTCATTTACTAAAACTAAAATAATCTACATCCAAAACACAATTTATTATAACCTTACTATAATAATCTAAGACTATAATAACCAACTCTTTATCCCAAACGTCTTCTTAATGTTTTAGACAACAAATTAGTATTATTATTATTTTATGTATTTAAATTAATAATTTAAAAAATACTTAAGACTATAGTAACCAACTCCTTATTTCAAACGTCCCTTTAATATTTTAGACAATAAATTAGTATTATTATTATTTTATGTATTTAAATTAGTAGTTTTAAAAAATTATACTTTTTTGCTGTAATTTTAATATAAAGTTAAAGTTAATTATATTATAAAAAGAAATTAATAAAAAATTAATAAAAACAGTATGTCAAGCTACTGTTATATAAAATTTATAATAACATTAAGCCAACCTTGTTGTTGTTATTATTATATTATTATTATTATTATTTATTATTATTATTATTATTATTATTATTATATATATATATAATAATATAATAATAATAATTAATAATAATTGGGATGGGGTTGGGTCAAGGATGGAGTGGAATACTCTCCCTATTTTCGATTGGGGACCTATTTTGTGTTCTCGGTTTGACCTATCCTTGTTTAAATTGGGGATTCTTCATCCCGATCGAAATGATTATCTGTAAGAATTTTTACCAATCATAATCATAGTCTATTGTTGATAAATAGTAAGATTTTGTTATATTTAGAAAGAAGTTAATTTGTTTTTCTTTATTTCAAAATAACAGCGGACATAAGATAAGGAAGATAATTAATGGCTTAATTTGAAGAATGGTAGGTTGGATGTACATGTAAAATCGTGAGGAAGTGGAAAGTAGAAGTTGGGCTCAAATTTCACATTGTCCCATCAATTATTTCGTACAAACTACACCTGAGATGGACGTTTGAAAACGACCATTCAATTTAATTCCACTAATATAATATTAAATCAGTAATTAATTTGATTTATGAATAATTTAAAGCACTTGTGTTTCACAGGTTCCAAGTAGAGTTTTGAAAAATCTTTAGCGTATGTTGCATTTATCTTTTATCTCTGTGCAATTTATTCCTTCTGTCCAGCCATGATTTGTTATGAATTTATAATGACAACATTTTTTAATATATAATTTTTAGTGATTTTCTTTTTGTTGGGCTCACTACGATAAACAAAGGGTGAAGATGCATGAAAAGGATGGGTTTAGAATGACTCAAAAAAAGCTTTTCAAAAATCCTATAAAAAAGGGTGAAGATGCATGAAAAGGATGTATTTTTATTTAAGCATTCTTGATATAAAAAAAATCCTTAAAATAAAAATTCGTATGTGTTTGATAATTCTTTCTAAAAAGTGTTCTTATATACGAAATAATTGTACGAAAGGCACCTAATATAGGGGTCCAAATGAAAAGTAAACCAATATTTAAAAATCAATTAATTTTGGCCTTTTGTTTATGCCAGTACCATGAGAAACTACCACTTTAATTCCAATGCACTTCTTCCTTCTTCTTCGTTTTGCAGTTGCTCAATCTGCAAAAGAAAAAATTTTACATCTTTATTATGCGTTCATCTTCTTTTTTATCTTTTGCATTCTTCTTCTTCCGCTTCTTCTCCTTCTTCTGGTTCCTGTTAGTTCTACCGCGCCTTCTTTTTTTTTTTTCCTTCTTCTTCTTCCATTTTTTCCTGTGTTCTTCTTCTTCTTCTTCTTTTCCTACGCTTTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTTTCCCACGTGTTTCTTCTTCTTCTTTTCCAGCGCTTTTCTTCTTCTTCTTCCTTATTCTCCATTTTTTTTTCTTTTCCTTCTTCGTTTTGCAGTTTTTTTTTTCTGAAAAAGAAAATTACAGTTGTGTCTGATGAAGAACAAGAGGCCAAAACGAGAAGCCAGAGGAGAAGCAAGAGGAAGAATATGAACAAAAGGAAAAGTCGAAAAAAATTATAGTTATACCTGATGAGGAACAAGAAAAAGGAATGAGAAGTGAGAATTAGTTAAAGCGCCAGACCCTTCGTGCTTGATCTGTGGAGACAATATGGTATTTTCACACGGGATTGACGTAAACAGGAGGTCGTTTTCGAACAAATTTGAATCTTGGGCCAAATTTCATTTGGGACCCTAAAAAGAGACCTTTCATACAAAACCCCCTATATATACAAGTTTGTTTGATTTATTATATTCTAAAGGTATTTAGTAATGTCAAAACTATAAAGGAGGTGGCTAGCAGCAGTAGCACGAAGGTGGTGGCTAGAGGTGGCTGATGGCAGTTACAGAAGCTTGTCCGTGATAGTGGTCGGAGTAGGTTTTTAGTGGTGGCTGCCCACAAAGGATGGTGGTAGCCACGACAACATAGTGAAAGTGAGAATTAGGGAGATATTGACCATTAAACACTAGGAAAAAAAACAATCAAAAAGTTGGTTTCAAGTGTTTAGATTTGTTAGGATAGAGGTCATGCGAATGTCACAAAGTCAAACTCACTTTGACTTCTGAAAATATATCTTAATTTGTG

mRNA sequence

AAAACAACCTCGCCAACTCAATTCGAACCCTCTAGACCCTTTGAAGTTTGACCCTTCTCTTATAAGTTAACATTACATAAACTTACAAGTTACAATCCCCTTCATAACTCTACCTCCCTTCTTCTCTTTTTTCTTTGTTATTATTATGGGGACTTTAGTGGGTCATGTAGCGCCCGGGTTTGGGTTCTTCATCATTGGCCTATGGCATCTCTTCAACAATACCAAAATCGAAGCTCGTAACAACGATCCTCCAAATACCGTTAAATTCCTCTCATGGTTCCCCACTTCAAGATTCAAGTACTTAGAGCCGTGTCTGATCATGGTTGGCGCCTCGGCCTCCATAGCCATGGAGTTATTCATCGGCCCACAACAGCACCAGCCGTTCGACACGGACGGCACCATCCCTTCCTACCATCTCCACAACTTCGAACACTCCAACATTTCCATGACTTTCTTTGTGTTTGCTGCCTTTTGCGTTGTTTTGGATCGGCTTCGTCCGAAAGCTTATTTTGAGCTGACACAGTTTGTTGGGGGTGTGGCTTTTGGGCAAGAGCTTCTCTTGTTTCACCTTCACTCGGCTGACCATATGGGCCCTGAAGGACAATACCACTTGCTTCTTCAGCTTCTTGTGTTTGTGTCTTTTGCCACAACTCTCCTTGGGATTGCTTTTCCCACTAGCTTCTTGGTGGCGTTTGTGAGATCTCTTAGCATTGTTTTTCAAGGCCTTTGGCTTATGCTCATGGGCTTCGCGCTTTGGACTCCTTCTTTCATTCCTAAAGGTTGCTTTATGCACTTGGAAGATGGGCACAAGGTGGTGCGTTGCGCCGGGGAGGAGCCACTTCAGCGAGCCAAGGCGCTGATCAACCTTCAGTTCAGCTGGCTGTTAATTGCACTCACCATTTTCGCTTTATCATTTCATTTGCTTCTGCTTAAACTATACCAACAAGAAACGCCCAAGAACGCCGCCCACTACTTTTCATTAATGACCGACGACGAAGAAAAAGCATTAGAAGCAGCTGCCTCAACAAAACTAACACCATACGACGTCGCTTCCAGGAGTTTCATTCAAATGGGCCACAAGTTCACGGAAACGGACATGGAAAGTTTTTTTTTTCTGAAAAAGAAAATTACAGTTGTGTCTGATGAAGAACAAGAGGCCAAAACGAGAAGCCAGAGGAGAAGCAAGAGGAAGAATATGAACAAAAGGAAAAGTCGAAAAAAATTATAGTTATACCTGATGAGGAACAAGAAAAAGGAATGAGAAGTGAGAATTAGTTAAAGCGCCAGACCCTTCGTGCTTGATCTGTGGAGACAATATGGTATTTTCACACGGGATTGACGTAAACAGGAGGTCGTTTTCGAACAAATTTGAATCTTGGGCCAAATTTCATTTGGGACCCTAAAAAGAGACCTTTCATACAAAACCCCCTATATATACAAGTTTGTTTGATTTATTATATTCTAAAGGTATTTAGTAATGTCAAAACTATAAAGGAGGTGGCTAGCAGCAGTAGCACGAAGGTGGTGGCTAGAGGTGGCTGATGGCAGTTACAGAAGCTTGTCCGTGATAGTGGTCGGAGTAGGTTTTTAGTGGTGGCTGCCCACAAAGGATGGTGGTAGCCACGACAACATAGTGAAAGTGAGAATTAGGGAGATATTGACCATTAAACACTAGGAAAAAAAACAATCAAAAAGTTGGTTTCAAGTGTTTAGATTTGTTAGGATAGAGGTCATGCGAATGTCACAAAGTCAAACTCACTTTGACTTCTGAAAATATATCTTAATTTGTG

Coding sequence (CDS)

ATGGGGACTTTAGTGGGTCATGTAGCGCCCGGGTTTGGGTTCTTCATCATTGGCCTATGGCATCTCTTCAACAATACCAAAATCGAAGCTCGTAACAACGATCCTCCAAATACCGTTAAATTCCTCTCATGGTTCCCCACTTCAAGATTCAAGTACTTAGAGCCGTGTCTGATCATGGTTGGCGCCTCGGCCTCCATAGCCATGGAGTTATTCATCGGCCCACAACAGCACCAGCCGTTCGACACGGACGGCACCATCCCTTCCTACCATCTCCACAACTTCGAACACTCCAACATTTCCATGACTTTCTTTGTGTTTGCTGCCTTTTGCGTTGTTTTGGATCGGCTTCGTCCGAAAGCTTATTTTGAGCTGACACAGTTTGTTGGGGGTGTGGCTTTTGGGCAAGAGCTTCTCTTGTTTCACCTTCACTCGGCTGACCATATGGGCCCTGAAGGACAATACCACTTGCTTCTTCAGCTTCTTGTGTTTGTGTCTTTTGCCACAACTCTCCTTGGGATTGCTTTTCCCACTAGCTTCTTGGTGGCGTTTGTGAGATCTCTTAGCATTGTTTTTCAAGGCCTTTGGCTTATGCTCATGGGCTTCGCGCTTTGGACTCCTTCTTTCATTCCTAAAGGTTGCTTTATGCACTTGGAAGATGGGCACAAGGTGGTGCGTTGCGCCGGGGAGGAGCCACTTCAGCGAGCCAAGGCGCTGATCAACCTTCAGTTCAGCTGGCTGTTAATTGCACTCACCATTTTCGCTTTATCATTTCATTTGCTTCTGCTTAAACTATACCAACAAGAAACGCCCAAGAACGCCGCCCACTACTTTTCATTAATGACCGACGACGAAGAAAAAGCATTAGAAGCAGCTGCCTCAACAAAACTAACACCATACGACGTCGCTTCCAGGAGTTTCATTCAAATGGGCCACAAGTTCACGGAAACGGACATGGAAAGTTTTTTTTTTCTGAAAAAGAAAATTACAGTTGTGTCTGATGAAGAACAAGAGGCCAAAACGAGAAGCCAGAGGAGAAGCAAGAGGAAGAATATGAACAAAAGGAAAAGTCGAAAAAAATTATAG

Protein sequence

MGTLVGHVAPGFGFFIIGLWHLFNNTKIEARNNDPPNTVKFLSWFPTSRFKYLEPCLIMVGASASIAMELFIGPQQHQPFDTDGTIPSYHLHNFEHSNISMTFFVFAAFCVVLDRLRPKAYFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVFVSFATTLLGIAFPTSFLVAFVRSLSIVFQGLWLMLMGFALWTPSFIPKGCFMHLEDGHKVVRCAGEEPLQRAKALINLQFSWLLIALTIFALSFHLLLLKLYQQETPKNAAHYFSLMTDDEEKALEAAASTKLTPYDVASRSFIQMGHKFTETDMESFFFLKKKITVVSDEEQEAKTRSQRRSKRKNMNKRKSRKKL
Homology
BLAST of Bhi10G000334 vs. TAIR 10
Match: AT1G55230.1 (Family of unknown function (DUF716) )

HSP 1 Score: 344.0 bits (881), Expect = 1.5e-94
Identity = 169/286 (59.09%), Postives = 214/286 (74.83%), Query Frame = 0

Query: 1   MGTLVGHVAPGFGFFIIGLWHLFNNTKIEARNNDPPNTVKFLSWFPTSRFKYLEPCLIMV 60
           MGTLVGHVAPGF FF +GLWHLFNN K+   N   PNT     WFPTS+ ++LE  +IMV
Sbjct: 1   MGTLVGHVAPGFAFFALGLWHLFNNIKLFCLN---PNTFTSSPWFPTSKLRHLELYIIMV 60

Query: 61  GASASIAMELFIGPQQHQPFDTDGTIPSYHLHNFEHSNISMTFFVFAAFCVVLDRLRPKA 120
            +S SIA ELFIGP++HQPFD+DGTIPS HLHNFEHS+ISM+F V+A F VVLD+ R ++
Sbjct: 61  CSSISIASELFIGPKKHQPFDSDGTIPSNHLHNFEHSSISMSFLVYAIFAVVLDQERRRS 120

Query: 121 YFE--LTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVFVSFATTLLGIAFPTS 180
                LT  V   AF Q+L LFHLHSADHMG EGQYH LLQL++FVSF TTL+GI  P S
Sbjct: 121 NISHGLTMLVASSAFAQQLFLFHLHSADHMGIEGQYHKLLQLIIFVSFLTTLIGITLPKS 180

Query: 181 FLVAFVRSLSIVFQGLWLMLMGFALWTPSFIPKGCFMHLEDGHKVVRCAGEEPLQRAKAL 240
           FLV+FVRS SI FQG+W ++MG+ LWTPS IPKGCF+H E+GH+V++C+ ++ + RAK+L
Sbjct: 181 FLVSFVRSSSITFQGVWFVVMGYMLWTPSLIPKGCFLHEEEGHQVIKCSSDKAIHRAKSL 240

Query: 241 INLQFSWLLIALTIFALSFHLLLLKLYQQETPKNAAHYFSLMTDDE 285
           +N++FSW  + +TIF +S  L+L  LY +      A Y  L+T D+
Sbjct: 241 VNIEFSWFFVGITIFVMSLFLILSGLYGEN-----AEYSILVTKDK 278

BLAST of Bhi10G000334 vs. TAIR 10
Match: AT1G55240.1 (Family of unknown function (DUF716) )

HSP 1 Score: 320.9 bits (821), Expect = 1.3e-87
Identity = 164/318 (51.57%), Postives = 220/318 (69.18%), Query Frame = 0

Query: 1   MGTLVGHVAPGFGFFIIGLWHLFNNTKIEARNNDPPNTVKFLSWFPTSRFKYLEPCLIMV 60
           MG+ +GHV PGF F  +GLWHLFNN K+   +   PNT    +WFP S+ ++LE  LIM+
Sbjct: 1   MGSFLGHVLPGFAFVALGLWHLFNNIKLFCLH---PNTFSSSTWFPISKLRHLELYLIML 60

Query: 61  GASASIAMELFIGPQQHQPFDTDGTIPSYHLHNFEHSNISMTFFVFAAFCVVLDRLRPKA 120
            ++ASI+MELFIGP++H PFD DGTIPS HLHNFEHS+ISM+F V+A   +VLDR RP+A
Sbjct: 61  SSTASISMELFIGPRRHHPFDVDGTIPSNHLHNFEHSSISMSFLVYAVLALVLDRARPRA 120

Query: 121 YFE--LTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVFVSFATTLLGIAFPTS 180
                LT      AF Q+LLLFH HS DHMG EGQYH++LQ+++FVS  TT++GI  P S
Sbjct: 121 AASEGLTMLAAAAAFSQQLLLFHFHSTDHMGVEGQYHVILQVVIFVSLLTTIMGIFLPKS 180

Query: 181 FLVAFVRSLSIVFQGLWLMLMGFALWTPSFIPKGCFMHLEDGHKVVRCAGEEPLQRAKAL 240
           FLV+ VRS SI FQG+WL+++G  L+TPS IPKGC++H E  H +V+C+ EE L RAK+L
Sbjct: 181 FLVSLVRSSSIAFQGVWLIVIGCMLYTPSLIPKGCYIHDEGRHIIVKCSTEEALHRAKSL 240

Query: 241 INLQFSWLLIALTIFALSFHLLLLKLYQQETPKNAAHYFSLMT------DDEEKALEAAA 300
           +NL+FSWL +  T+F ++ +L+L ++Y +        Y SL T      DDEE+  E+ +
Sbjct: 241 VNLEFSWLFVTNTLFVVTLYLILDRVYGEN-----VEYSSLTTNNQTYQDDEEQHFESKS 300

Query: 301 STKLTPYDVASRSFIQMG 311
           + K         SF+QMG
Sbjct: 301 TQK--------TSFVQMG 302

BLAST of Bhi10G000334 vs. TAIR 10
Match: AT5G19870.1 (Family of unknown function (DUF716) )

HSP 1 Score: 314.3 bits (804), Expect = 1.3e-85
Identity = 153/284 (53.87%), Postives = 203/284 (71.48%), Query Frame = 0

Query: 1   MGTLVGHVAPGFGFFIIGLWHLFNNTKIEARNNDPPNTVKFLSWFPTSRFKYLEPCLIMV 60
           MGTLVGH+ PGF FF +GLWHLFN+ KI + +   P +     WFP S+ +YLEP +I++
Sbjct: 1   MGTLVGHILPGFFFFALGLWHLFNHIKIFSLH---PKSYIAPLWFPLSKPRYLEPLVIII 60

Query: 61  GASASIAMELFIGPQQHQPFD-TDGTIPSYHLHNFEHSNISMTFFVFAAFCVVLDRLRPK 120
            +S SI++ELF+G + HQPFD  DGTIPS HLHNFEHS IS TF  +AAF +V D+ RP 
Sbjct: 61  ASSLSISVELFLGQKNHQPFDPNDGTIPSNHLHNFEHSCISFTFITYAAFAIVFDKTRPI 120

Query: 121 AYFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVFVSFATTLLGIAFPTSF 180
           A+  L   +  +AF Q+L LFH HS+DH G EG YHLLLQL+VFVS  TTLLGIA P+SF
Sbjct: 121 AHRSLINLIAALAFAQQLFLFHFHSSDHTGVEGHYHLLLQLVVFVSLVTTLLGIALPSSF 180

Query: 181 LVAFVRSLSIVFQGLWLMLMGFALWTPSFIPKGCFMHLEDGHKVVRCAGEEPLQRAKALI 240
           +++FVRSLS+ FQG+WLM M   LWTPS +PK CF+H+E+G   +RC+  + L RA +L+
Sbjct: 181 ILSFVRSLSVSFQGIWLMSMACMLWTPSLVPKDCFLHIEEGKHTIRCSDVKALHRAISLV 240

Query: 241 NLQFSWLLIALTIFALSFHLLLLKLYQQETPKNAAHYFSLMTDD 284
           N+QFSW L+ +TIFA+ F++ L ++Y ++       Y  L T D
Sbjct: 241 NIQFSWFLVIITIFAMWFYIFLQRIYGEK-----IEYSQLRTKD 276

BLAST of Bhi10G000334 vs. TAIR 10
Match: AT1G49470.1 (Family of unknown function (DUF716) )

HSP 1 Score: 166.0 bits (419), Expect = 5.5e-41
Identity = 98/270 (36.30%), Postives = 136/270 (50.37%), Query Frame = 0

Query: 1   MGTLVGHVAPGFGFFIIGLWHLFNNTKIEARNNDPPNTVKFLSWFPTSRFKYLEPCLIMV 60
           MGT +GH  PG    ++GLWHLFN  +          + KF  WFP  + K+LE  LI+ 
Sbjct: 1   MGTFLGHFVPGLSLALLGLWHLFNTIRSYCLKGPEAFSAKF--WFPFPKLKHLELILILF 60

Query: 61  GASASIAMELFIGPQQHQPFDTDGTIPSYHLHNFEHSNISMTFFVFAAFCVVLD-RLRPK 120
            +  SI +     P     F+     P     N EH+++ +   +FA F +  +  L   
Sbjct: 61  FSFLSITLLTLDFPN----FNFSSLKPD----NLEHASMFLHLIIFACFALFCELTLCSD 120

Query: 121 AYFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVFVSFATTLLGIAFPTSF 180
            +  L   +    F QEL L H HS DH G EG YH LLQL+ FVSF++ L   +FP SF
Sbjct: 121 LFSGLIGVLSASVFAQELFLLHFHSTDHSGLEGHYHFLLQLIAFVSFSSALASASFPKSF 180

Query: 181 LVAFVRSLSIVFQGLWLMLMGFALWTPSFIPKGCFMHLE---DGHK-------VVRCAGE 240
             A    +S++FQG W + MGF LW P ++P+GC  ++    D ++        V C   
Sbjct: 181 SAALFLPISVMFQGCWFLNMGFMLWIPEYVPRGCVSNMSTSTDNNRRSVYHSGAVACESP 240

Query: 241 EPLQRAKALINLQFSWLLIALTIFALSFHL 260
               RAKAL NLQFSW+L A+ I   +  L
Sbjct: 241 GAEIRAKALANLQFSWMLSAILIITCALCL 260

BLAST of Bhi10G000334 vs. TAIR 10
Match: AT1G32120.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: membrane; EXPRESSED IN: 14 plant structures; EXPRESSED DURING: 4 anthesis, C globular stage, F mature embryo stage, petal differentiation and expansion stage, E expanded cotyledon stage; CONTAINS InterPro DOMAIN/s: Aminotransferase-like, plant mobile domain (InterPro:IPR019557), Protein of unknown function DUF716 (InterPro:IPR006904); BEST Arabidopsis thaliana protein match is: Aminotransferase-like, plant mobile domain family protein (TAIR:AT1G51538.1); Has 16736 Blast hits to 9656 proteins in 576 species: Archae - 4; Bacteria - 1182; Metazoa - 7098; Fungi - 2631; Plants - 1178; Viruses - 174; Other Eukaryotes - 4469 (source: NCBI BLink). )

HSP 1 Score: 119.4 bits (298), Expect = 6.0e-27
Identity = 78/258 (30.23%), Postives = 122/258 (47.29%), Query Frame = 0

Query: 1    MGTLVGHVAPGFGFFIIGLWHLFNNTKIEARNNDPPNTVKFLSWFP----TSRFKYLEPC 60
            MG+  GH  PG  F ++G+WH++++     R    P++ +   W P      R KYLE  
Sbjct: 943  MGSFKGHALPGTLFLVVGVWHIWSSV---VRYISNPSSFRVRVWHPVPGFNDRIKYLELY 1002

Query: 61   LIMVGASASIAMELFIGPQQHQPFDTDGTIPSYHLHNFEHSNISMTFFV--FAAFCVVLD 120
            ++ +G+   + +E       H  F  +G +   H+++FEHS + + FF+  F A      
Sbjct: 1003 VVTIGSFIDLCIEFLY--STHLKFFVNGVLNPSHMNDFEHSGMLLMFFILGFIALLSEKT 1062

Query: 121  RLRPKAYFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVFVSFATTLLGIA 180
            RL P    E    +   AF  E LLF  HS  H G EG YHLLL  L+ +   +++ G  
Sbjct: 1063 RLLPLPQ-EALCLIAATAFTAECLLFFFHSTSHKGLEGYYHLLLVFLIGLCVISSIAGAI 1122

Query: 181  FPTSFLVAFVRSLSIVFQGLWLMLMGFALWTPSFIPKGCFMHLEDGHKVVRCAGEEPLQR 240
             PTSF V     +++  QGLW     F L+ P  +P+GC +        V C   +    
Sbjct: 1123 CPTSFPVDLCNGIAMTLQGLWFYQTAFTLYGP-MMPQGCSLK----QNSVVCRSVDSEVS 1182

Query: 241  AKALINLQFSWLLIALTI 253
             + L N Q   L++A+ +
Sbjct: 1183 GEFLANFQLFSLVLAVLV 1189

BLAST of Bhi10G000334 vs. ExPASy Swiss-Prot
Match: Q9NWC5 (Transmembrane protein 45A OS=Homo sapiens OX=9606 GN=TMEM45A PE=1 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 3.1e-04
Identity = 61/224 (27.23%), Postives = 86/224 (38.39%), Query Frame = 0

Query: 1   MGTLVGHVAPGFGFFIIGLWHLFNNTKIEARNNDPPNTVKFLSWFPTSRFKYLEPCLIMV 60
           MG   GH  PG  FFIIGLW     + ++        T    S     R + LE   I+ 
Sbjct: 1   MGNFRGHALPGTFFFIIGLWWC-TKSILKYICKKQKRTCYLGSKTLFYRLEILEGITIVG 60

Query: 61  GASASIAMELFIGPQQHQPFDTDGTIPSYHLHNFEHSNISMTFF-----VFAAFCVVLDR 120
            A   +A E FI    H             L  + H   +M FF     V    C  +  
Sbjct: 61  MALTGMAGEQFIPGGPHLMLYDYKQGHWNQLLGWHH--FTMYFFFGLLGVADILCFTISS 120

Query: 121 LRPKAYFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVFVSFATTLLGIAF 180
           L       LT+ +   A   E  +F+ H+      +   H LL L+VF++     L    
Sbjct: 121 LP----VSLTKLMLSNALFVEAFIFYNHTHGREMLDIFVHQLLVLVVFLTGLVAFLEFLV 180

Query: 181 PTSFLVAFVRSLSIVFQGLWLMLMGFALWTPSFIPKGCFMHLED 220
             + L+  +RS  I+ QG W   +GF L+ PS  P    M  E+
Sbjct: 181 RNNVLLELLRSSLILLQGSWFFQIGFVLYPPSGGPAWDLMDHEN 217

BLAST of Bhi10G000334 vs. NCBI nr
Match: XP_038901896.1 (transmembrane protein 45B-like [Benincasa hispida])

HSP 1 Score: 646.0 bits (1665), Expect = 2.0e-181
Identity = 319/319 (100.00%), Postives = 319/319 (100.00%), Query Frame = 0

Query: 1   MGTLVGHVAPGFGFFIIGLWHLFNNTKIEARNNDPPNTVKFLSWFPTSRFKYLEPCLIMV 60
           MGTLVGHVAPGFGFFIIGLWHLFNNTKIEARNNDPPNTVKFLSWFPTSRFKYLEPCLIMV
Sbjct: 1   MGTLVGHVAPGFGFFIIGLWHLFNNTKIEARNNDPPNTVKFLSWFPTSRFKYLEPCLIMV 60

Query: 61  GASASIAMELFIGPQQHQPFDTDGTIPSYHLHNFEHSNISMTFFVFAAFCVVLDRLRPKA 120
           GASASIAMELFIGPQQHQPFDTDGTIPSYHLHNFEHSNISMTFFVFAAFCVVLDRLRPKA
Sbjct: 61  GASASIAMELFIGPQQHQPFDTDGTIPSYHLHNFEHSNISMTFFVFAAFCVVLDRLRPKA 120

Query: 121 YFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVFVSFATTLLGIAFPTSFL 180
           YFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVFVSFATTLLGIAFPTSFL
Sbjct: 121 YFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVFVSFATTLLGIAFPTSFL 180

Query: 181 VAFVRSLSIVFQGLWLMLMGFALWTPSFIPKGCFMHLEDGHKVVRCAGEEPLQRAKALIN 240
           VAFVRSLSIVFQGLWLMLMGFALWTPSFIPKGCFMHLEDGHKVVRCAGEEPLQRAKALIN
Sbjct: 181 VAFVRSLSIVFQGLWLMLMGFALWTPSFIPKGCFMHLEDGHKVVRCAGEEPLQRAKALIN 240

Query: 241 LQFSWLLIALTIFALSFHLLLLKLYQQETPKNAAHYFSLMTDDEEKALEAAASTKLTPYD 300
           LQFSWLLIALTIFALSFHLLLLKLYQQETPKNAAHYFSLMTDDEEKALEAAASTKLTPYD
Sbjct: 241 LQFSWLLIALTIFALSFHLLLLKLYQQETPKNAAHYFSLMTDDEEKALEAAASTKLTPYD 300

Query: 301 VASRSFIQMGHKFTETDME 320
           VASRSFIQMGHKFTETDME
Sbjct: 301 VASRSFIQMGHKFTETDME 319

BLAST of Bhi10G000334 vs. NCBI nr
Match: XP_008449792.1 (PREDICTED: transmembrane protein 45A [Cucumis melo])

HSP 1 Score: 555.8 bits (1431), Expect = 2.7e-154
Identity = 277/323 (85.76%), Postives = 294/323 (91.02%), Query Frame = 0

Query: 1   MGTLVGHVAPGFGFFIIGLWHLFNNTKIEA---RNNDPPNTVKFLSWFPTSRFKYLEPCL 60
           MGTLVGHVAPGFGFFIIGLWHLFNN KIEA    NN+ PNT  FLSWFPTSRFKYLEP L
Sbjct: 1   MGTLVGHVAPGFGFFIIGLWHLFNNIKIEACNNNNNNHPNTAIFLSWFPTSRFKYLEPFL 60

Query: 61  IMVGASASIAMELFIGPQQHQPFDTDGTIPSYHLHNFEHSNISMTFFVFAAFCVVLDRLR 120
           IMVGASASIAMELFIGPQ+HQPFDTDGTIPSYHLHNFEHSNISMTFFVFAAFCVVLDR+R
Sbjct: 61  IMVGASASIAMELFIGPQRHQPFDTDGTIPSYHLHNFEHSNISMTFFVFAAFCVVLDRVR 120

Query: 121 PKAYFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVFVSFATTLLGIAFPT 180
           PKAYFELTQFVG VAFGQELLLFHLHSADHMGPEGQYHLLLQLLV VSFATTLLGIA P 
Sbjct: 121 PKAYFELTQFVGTVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVLVSFATTLLGIALPN 180

Query: 181 SFLVAFVRSLSIVFQGLWLMLMGFALWTPSFIPKGCFMHLEDGHKVVRCAGEEPLQRAKA 240
           SFLVAFVRSLSIVFQGLWL+LMGFALWTPSFIPKGCFMHLEDGHKVVRCAG+EPL RAK+
Sbjct: 181 SFLVAFVRSLSIVFQGLWLILMGFALWTPSFIPKGCFMHLEDGHKVVRCAGDEPLHRAKS 240

Query: 241 LINLQFSWLLIALTIFALSFHLLLLKLYQQETPK-NAAHYFSLMTDDEEKALEAAASTKL 300
           LIN+QFSWLLIALTI  LSF+L+LLKLYQQ+TPK N   YFSL+T+D+E+A   +  TK 
Sbjct: 241 LINIQFSWLLIALTILGLSFYLVLLKLYQQQTPKINPVQYFSLITNDDEEAAATSTPTKT 300

Query: 301 TPYDVASRSFIQMGHKFTETDME 320
           T YDV S+SFIQMGHK T+TDME
Sbjct: 301 TSYDVVSKSFIQMGHKLTDTDME 323

BLAST of Bhi10G000334 vs. NCBI nr
Match: XP_004142158.1 (transmembrane protein 45A [Cucumis sativus] >KGN54083.1 hypothetical protein Csa_018130 [Cucumis sativus])

HSP 1 Score: 543.1 bits (1398), Expect = 1.8e-150
Identity = 276/328 (84.15%), Postives = 295/328 (89.94%), Query Frame = 0

Query: 1   MGTLVGHVAPGFGFFIIGLWHLFNNTKIEARNNDPPNTVKFLSWFPTSRFKYLEPCLIMV 60
           MGTLVGHVAPGFGFFIIGLWHLFNN KIEA NN+PPNT  FLSWFPTSRFKYLEP LIMV
Sbjct: 1   MGTLVGHVAPGFGFFIIGLWHLFNNIKIEACNNNPPNTAIFLSWFPTSRFKYLEPFLIMV 60

Query: 61  GASASIAMELFIGPQQHQPFDTDGTIPSYHLHNFEHSNISMTFFVFAAFCVVLDRLRPKA 120
           GASASIAMELFIGPQ+HQPFD DGTIPSYHLHNFEHSNISMTFFVFAAFCVVLD++RPKA
Sbjct: 61  GASASIAMELFIGPQRHQPFDIDGTIPSYHLHNFEHSNISMTFFVFAAFCVVLDQVRPKA 120

Query: 121 YFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVFVSFATTLLGIAFPTSFL 180
           YFELTQFVG VAFGQELLLFHLHSADHMGPEGQYHLLLQLLV VSFATTLLGIA P SFL
Sbjct: 121 YFELTQFVGTVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVVVSFATTLLGIALPNSFL 180

Query: 181 VAFVRSLSIVFQGLWLMLMGFALWTPSFIPKGCFMHLEDGHKVVRCAGEEPLQRAKALIN 240
           +AFVRSLSIVFQGLWL+LMGFALWTPSFIPKGCFMHLEDGHKVVRCAG+EPL RAK+LIN
Sbjct: 181 LAFVRSLSIVFQGLWLILMGFALWTPSFIPKGCFMHLEDGHKVVRCAGDEPLHRAKSLIN 240

Query: 241 LQFSWLLIALTIFALSFHLLLLKLYQQETP----KNAAHYFSLMT-DDEEKALEAAAS-- 300
           +QFSWLLIALTI +LSF+L+LLKLYQQ+TP     N   YFSL+T DDEE+  EAA S  
Sbjct: 241 IQFSWLLIALTILSLSFYLVLLKLYQQQTPDPKKNNPVQYFSLITNDDEEEDAEAATSTP 300

Query: 301 TKLTPYDVAS--RSFIQMGHKFTETDME 320
           TK + Y+V S  +SFIQMGHK  +TDME
Sbjct: 301 TKTSSYNVVSVPKSFIQMGHKLADTDME 328

BLAST of Bhi10G000334 vs. NCBI nr
Match: KAG7010234.1 (hypothetical protein SDJN02_27026, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 503.8 bits (1296), Expect = 1.2e-138
Identity = 255/318 (80.19%), Postives = 274/318 (86.16%), Query Frame = 0

Query: 1   MGTLVGHVAPGFGFFIIGLWHLFNNTKIEARNNDPPNTVKFLSWFPTSRFKYLEPCLIMV 60
           MGTLVGHVAPGFGFFIIGLWHLFNN KIEARNN PPN+  FLSWFPTSRFKYLEP LIM+
Sbjct: 1   MGTLVGHVAPGFGFFIIGLWHLFNNIKIEARNN-PPNSPIFLSWFPTSRFKYLEPLLIML 60

Query: 61  GASASIAMELFIGPQQHQPFDTDGTIPSYHLHNFEHSNISMTFFVFAAFCVVLDRLRPKA 120
           GASAS+AMELFIGPQ+H PFDTDGTIPS HLHN EHSNISMTFFVFAAF V+LDRLRPKA
Sbjct: 61  GASASVAMELFIGPQRHHPFDTDGTIPSNHLHNIEHSNISMTFFVFAAFSVILDRLRPKA 120

Query: 121 YFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVFVSFATTLLGIAFPTSFL 180
           YFELTQF+G VAFGQELLLFHLHSADH GPEGQYH+LLQLLV +S ATTLLGIA PTSFL
Sbjct: 121 YFELTQFLGAVAFGQELLLFHLHSADHNGPEGQYHMLLQLLVLLSLATTLLGIALPTSFL 180

Query: 181 VAFVRSLSIVFQGLWLMLMGFALWTPSFIPKGCFMHLEDGHKVVRCAGEEPLQRAKALIN 240
           VAF+RS SI+FQG+WLMLMGF LWTPS I KGCFMHLEDGHKVVRC GE PL RAK+LIN
Sbjct: 181 VAFIRSFSIIFQGVWLMLMGFTLWTPSLISKGCFMHLEDGHKVVRCGGEAPLHRAKSLIN 240

Query: 241 LQFSWLLIALTIFALSFHLLLLKLYQQETPKNAAHYFSLMTDDEEKALEAAASTKLTPYD 300
           +QFSWLLIALTIFALSFHL LLKLYQQ TPKN   Y SL+T++EE+             D
Sbjct: 241 IQFSWLLIALTIFALSFHLFLLKLYQQ-TPKN-PQYISLVTNEEEE-------EPSNNID 300

Query: 301 VASRSFIQMGHKFTETDM 319
             +RSFI+MGHKFTE  M
Sbjct: 301 TRARSFIEMGHKFTEMGM 308

BLAST of Bhi10G000334 vs. NCBI nr
Match: XP_023513146.1 (transmembrane protein 45A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 496.1 bits (1276), Expect = 2.5e-136
Identity = 251/318 (78.93%), Postives = 272/318 (85.53%), Query Frame = 0

Query: 1   MGTLVGHVAPGFGFFIIGLWHLFNNTKIEARNNDPPNTVKFLSWFPTSRFKYLEPCLIMV 60
           MGTLVGHVAPGFGFFIIGLWHLFNN KIEAR N PP++  FLSWFPTSRFKYLEP LIM+
Sbjct: 1   MGTLVGHVAPGFGFFIIGLWHLFNNIKIEARTN-PPHSPIFLSWFPTSRFKYLEPLLIML 60

Query: 61  GASASIAMELFIGPQQHQPFDTDGTIPSYHLHNFEHSNISMTFFVFAAFCVVLDRLRPKA 120
            ASAS+AMELFIGPQ+H PFDTDGTIPS HLHN EHSNISMTFFVFAAF V+LDRLRPKA
Sbjct: 61  AASASVAMELFIGPQRHHPFDTDGTIPSNHLHNIEHSNISMTFFVFAAFSVILDRLRPKA 120

Query: 121 YFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVFVSFATTLLGIAFPTSFL 180
           YFELTQF+G VAFGQELLLFHLHSADH GPEGQYH+LLQLLV +S ATTLLGIA PTSFL
Sbjct: 121 YFELTQFLGAVAFGQELLLFHLHSADHNGPEGQYHMLLQLLVLLSLATTLLGIALPTSFL 180

Query: 181 VAFVRSLSIVFQGLWLMLMGFALWTPSFIPKGCFMHLEDGHKVVRCAGEEPLQRAKALIN 240
           VAFVRS S++FQG+WLMLMGF LWTPS I KGCFMHLEDGHKVVRC GE PL RAK+L+N
Sbjct: 181 VAFVRSFSVIFQGVWLMLMGFTLWTPSLISKGCFMHLEDGHKVVRCGGEAPLHRAKSLVN 240

Query: 241 LQFSWLLIALTIFALSFHLLLLKLYQQETPKNAAHYFSLMTDDEEKALEAAASTKLTPYD 300
           LQFSWLLIALTIFALS HL LLKLYQQ TPKN   Y SL+T++EE+       T+     
Sbjct: 241 LQFSWLLIALTIFALSLHLFLLKLYQQ-TPKN-PQYISLVTNEEEEETSKNIDTR----- 300

Query: 301 VASRSFIQMGHKFTETDM 319
             +RSFI+MGHKFTE  M
Sbjct: 301 --ARSFIEMGHKFTEMGM 308

BLAST of Bhi10G000334 vs. ExPASy TrEMBL
Match: A0A1S3BMV5 (transmembrane protein 45A OS=Cucumis melo OX=3656 GN=LOC103491576 PE=3 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 1.3e-154
Identity = 277/323 (85.76%), Postives = 294/323 (91.02%), Query Frame = 0

Query: 1   MGTLVGHVAPGFGFFIIGLWHLFNNTKIEA---RNNDPPNTVKFLSWFPTSRFKYLEPCL 60
           MGTLVGHVAPGFGFFIIGLWHLFNN KIEA    NN+ PNT  FLSWFPTSRFKYLEP L
Sbjct: 1   MGTLVGHVAPGFGFFIIGLWHLFNNIKIEACNNNNNNHPNTAIFLSWFPTSRFKYLEPFL 60

Query: 61  IMVGASASIAMELFIGPQQHQPFDTDGTIPSYHLHNFEHSNISMTFFVFAAFCVVLDRLR 120
           IMVGASASIAMELFIGPQ+HQPFDTDGTIPSYHLHNFEHSNISMTFFVFAAFCVVLDR+R
Sbjct: 61  IMVGASASIAMELFIGPQRHQPFDTDGTIPSYHLHNFEHSNISMTFFVFAAFCVVLDRVR 120

Query: 121 PKAYFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVFVSFATTLLGIAFPT 180
           PKAYFELTQFVG VAFGQELLLFHLHSADHMGPEGQYHLLLQLLV VSFATTLLGIA P 
Sbjct: 121 PKAYFELTQFVGTVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVLVSFATTLLGIALPN 180

Query: 181 SFLVAFVRSLSIVFQGLWLMLMGFALWTPSFIPKGCFMHLEDGHKVVRCAGEEPLQRAKA 240
           SFLVAFVRSLSIVFQGLWL+LMGFALWTPSFIPKGCFMHLEDGHKVVRCAG+EPL RAK+
Sbjct: 181 SFLVAFVRSLSIVFQGLWLILMGFALWTPSFIPKGCFMHLEDGHKVVRCAGDEPLHRAKS 240

Query: 241 LINLQFSWLLIALTIFALSFHLLLLKLYQQETPK-NAAHYFSLMTDDEEKALEAAASTKL 300
           LIN+QFSWLLIALTI  LSF+L+LLKLYQQ+TPK N   YFSL+T+D+E+A   +  TK 
Sbjct: 241 LINIQFSWLLIALTILGLSFYLVLLKLYQQQTPKINPVQYFSLITNDDEEAAATSTPTKT 300

Query: 301 TPYDVASRSFIQMGHKFTETDME 320
           T YDV S+SFIQMGHK T+TDME
Sbjct: 301 TSYDVVSKSFIQMGHKLTDTDME 323

BLAST of Bhi10G000334 vs. ExPASy TrEMBL
Match: A0A0A0KX38 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G280600 PE=3 SV=1)

HSP 1 Score: 543.1 bits (1398), Expect = 8.7e-151
Identity = 276/328 (84.15%), Postives = 295/328 (89.94%), Query Frame = 0

Query: 1   MGTLVGHVAPGFGFFIIGLWHLFNNTKIEARNNDPPNTVKFLSWFPTSRFKYLEPCLIMV 60
           MGTLVGHVAPGFGFFIIGLWHLFNN KIEA NN+PPNT  FLSWFPTSRFKYLEP LIMV
Sbjct: 1   MGTLVGHVAPGFGFFIIGLWHLFNNIKIEACNNNPPNTAIFLSWFPTSRFKYLEPFLIMV 60

Query: 61  GASASIAMELFIGPQQHQPFDTDGTIPSYHLHNFEHSNISMTFFVFAAFCVVLDRLRPKA 120
           GASASIAMELFIGPQ+HQPFD DGTIPSYHLHNFEHSNISMTFFVFAAFCVVLD++RPKA
Sbjct: 61  GASASIAMELFIGPQRHQPFDIDGTIPSYHLHNFEHSNISMTFFVFAAFCVVLDQVRPKA 120

Query: 121 YFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVFVSFATTLLGIAFPTSFL 180
           YFELTQFVG VAFGQELLLFHLHSADHMGPEGQYHLLLQLLV VSFATTLLGIA P SFL
Sbjct: 121 YFELTQFVGTVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVVVSFATTLLGIALPNSFL 180

Query: 181 VAFVRSLSIVFQGLWLMLMGFALWTPSFIPKGCFMHLEDGHKVVRCAGEEPLQRAKALIN 240
           +AFVRSLSIVFQGLWL+LMGFALWTPSFIPKGCFMHLEDGHKVVRCAG+EPL RAK+LIN
Sbjct: 181 LAFVRSLSIVFQGLWLILMGFALWTPSFIPKGCFMHLEDGHKVVRCAGDEPLHRAKSLIN 240

Query: 241 LQFSWLLIALTIFALSFHLLLLKLYQQETP----KNAAHYFSLMT-DDEEKALEAAAS-- 300
           +QFSWLLIALTI +LSF+L+LLKLYQQ+TP     N   YFSL+T DDEE+  EAA S  
Sbjct: 241 IQFSWLLIALTILSLSFYLVLLKLYQQQTPDPKKNNPVQYFSLITNDDEEEDAEAATSTP 300

Query: 301 TKLTPYDVAS--RSFIQMGHKFTETDME 320
           TK + Y+V S  +SFIQMGHK  +TDME
Sbjct: 301 TKTSSYNVVSVPKSFIQMGHKLADTDME 328

BLAST of Bhi10G000334 vs. ExPASy TrEMBL
Match: A0A6J1JGR5 (transmembrane protein 45A-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111484363 PE=3 SV=1)

HSP 1 Score: 495.4 bits (1274), Expect = 2.1e-136
Identity = 250/318 (78.62%), Postives = 272/318 (85.53%), Query Frame = 0

Query: 1   MGTLVGHVAPGFGFFIIGLWHLFNNTKIEARNNDPPNTVKFLSWFPTSRFKYLEPCLIMV 60
           MGTLVGHVAPGFGFFIIGLWHLFNN KIEAR++ PPN+  FLSWFPTSRFKYLEP LIM+
Sbjct: 1   MGTLVGHVAPGFGFFIIGLWHLFNNMKIEARSS-PPNSPVFLSWFPTSRFKYLEPLLIML 60

Query: 61  GASASIAMELFIGPQQHQPFDTDGTIPSYHLHNFEHSNISMTFFVFAAFCVVLDRLRPKA 120
           GASAS+AMELFIGPQ+H PFDTDGTIPS HLHN EHSNISMTFFVFAAF V+LDRLRPKA
Sbjct: 61  GASASVAMELFIGPQRHHPFDTDGTIPSNHLHNIEHSNISMTFFVFAAFSVILDRLRPKA 120

Query: 121 YFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVFVSFATTLLGIAFPTSFL 180
           YFELTQF+G VAFGQELLLFHLHSADH GPEGQYH+LLQLLV +S ATTLLGIA PTSFL
Sbjct: 121 YFELTQFLGAVAFGQELLLFHLHSADHNGPEGQYHMLLQLLVLLSLATTLLGIALPTSFL 180

Query: 181 VAFVRSLSIVFQGLWLMLMGFALWTPSFIPKGCFMHLEDGHKVVRCAGEEPLQRAKALIN 240
           VAF+RS SI+FQG+WLMLMGF LWTPS I KGCFMHLEDGHKVVRC GE PL RAK+L+N
Sbjct: 181 VAFIRSFSIIFQGVWLMLMGFTLWTPSLISKGCFMHLEDGHKVVRCGGEAPLHRAKSLVN 240

Query: 241 LQFSWLLIALTIFALSFHLLLLKLYQQETPKNAAHYFSLMTDDEEKALEAAASTKLTPYD 300
           +QFSWLLIALTIFALS HL LLKLYQQ TPKN   Y SL+T + E+       T+     
Sbjct: 241 IQFSWLLIALTIFALSLHLFLLKLYQQ-TPKN-PQYISLITSEVEEETSKNIDTR----- 300

Query: 301 VASRSFIQMGHKFTETDM 319
             +RSFI+MGHKFTE  M
Sbjct: 301 --ARSFIEMGHKFTEMGM 308

BLAST of Bhi10G000334 vs. ExPASy TrEMBL
Match: A0A6J1FXU7 (transmembrane protein 45A-like OS=Cucurbita moschata OX=3662 GN=LOC111448740 PE=3 SV=1)

HSP 1 Score: 495.4 bits (1274), Expect = 2.1e-136
Identity = 250/315 (79.37%), Postives = 270/315 (85.71%), Query Frame = 0

Query: 1   MGTLVGHVAPGFGFFIIGLWHLFNNTKIEARNNDPPNTVKFLSWFPTSRFKYLEPCLIMV 60
           MGTLVGHVAPGFGFFIIGLWHLFNN KIEAR N PPN+  FLSWFPTSRFKYLEP LIM+
Sbjct: 1   MGTLVGHVAPGFGFFIIGLWHLFNNIKIEARTN-PPNSPIFLSWFPTSRFKYLEPLLIML 60

Query: 61  GASASIAMELFIGPQQHQPFDTDGTIPSYHLHNFEHSNISMTFFVFAAFCVVLDRLRPKA 120
           GASAS+AMELFIGPQ+H PFDTDGTIPS HLHN EHSNISMTFFVFAAF V+LDRLRPKA
Sbjct: 61  GASASVAMELFIGPQRHHPFDTDGTIPSNHLHNIEHSNISMTFFVFAAFSVILDRLRPKA 120

Query: 121 YFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVFVSFATTLLGIAFPTSFL 180
           YFELTQF+G VAFGQELLLFHLHSADH GPEGQYH+LLQLLV +S ATTLLGIA PTSFL
Sbjct: 121 YFELTQFLGAVAFGQELLLFHLHSADHNGPEGQYHMLLQLLVLLSLATTLLGIALPTSFL 180

Query: 181 VAFVRSLSIVFQGLWLMLMGFALWTPSFIPKGCFMHLEDGHKVVRCAGEEPLQRAKALIN 240
           VAF+RS SI+FQG+WLMLMGF LWTPS I KGCFMHLEDGHKVVRC GE P  RAK+LIN
Sbjct: 181 VAFIRSFSIIFQGVWLMLMGFTLWTPSLISKGCFMHLEDGHKVVRCGGEAPFHRAKSLIN 240

Query: 241 LQFSWLLIALTIFALSFHLLLLKLYQQETPKNAAHYFSLMTDDEEKALEAAASTKLTPYD 300
           +QFSWLL ALTIFALSFHL LLKLYQQ TPKN   Y SL+T++EE+       T+     
Sbjct: 241 IQFSWLLTALTIFALSFHLFLLKLYQQ-TPKN-PQYISLVTNEEEEETSKNIDTR----- 300

Query: 301 VASRSFIQMGHKFTE 316
             +RSFI+MGHKF E
Sbjct: 301 --ARSFIEMGHKFKE 305

BLAST of Bhi10G000334 vs. ExPASy TrEMBL
Match: A0A6J1JEQ6 (transmembrane protein 45A-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111484363 PE=3 SV=1)

HSP 1 Score: 494.2 bits (1271), Expect = 4.6e-136
Identity = 249/315 (79.05%), Postives = 271/315 (86.03%), Query Frame = 0

Query: 1   MGTLVGHVAPGFGFFIIGLWHLFNNTKIEARNNDPPNTVKFLSWFPTSRFKYLEPCLIMV 60
           MGTLVGHVAPGFGFFIIGLWHLFNN KIEAR++ PPN+  FLSWFPTSRFKYLEP LIM+
Sbjct: 1   MGTLVGHVAPGFGFFIIGLWHLFNNMKIEARSS-PPNSPVFLSWFPTSRFKYLEPLLIML 60

Query: 61  GASASIAMELFIGPQQHQPFDTDGTIPSYHLHNFEHSNISMTFFVFAAFCVVLDRLRPKA 120
           GASAS+AMELFIGPQ+H PFDTDGTIPS HLHN EHSNISMTFFVFAAF V+LDRLRPKA
Sbjct: 61  GASASVAMELFIGPQRHHPFDTDGTIPSNHLHNIEHSNISMTFFVFAAFSVILDRLRPKA 120

Query: 121 YFELTQFVGGVAFGQELLLFHLHSADHMGPEGQYHLLLQLLVFVSFATTLLGIAFPTSFL 180
           YFELTQF+G VAFGQELLLFHLHSADH GPEGQYH+LLQLLV +S ATTLLGIA PTSFL
Sbjct: 121 YFELTQFLGAVAFGQELLLFHLHSADHNGPEGQYHMLLQLLVLLSLATTLLGIALPTSFL 180

Query: 181 VAFVRSLSIVFQGLWLMLMGFALWTPSFIPKGCFMHLEDGHKVVRCAGEEPLQRAKALIN 240
           VAF+RS SI+FQG+WLMLMGF LWTPS I KGCFMHLEDGHKVVRC GE PL RAK+L+N
Sbjct: 181 VAFIRSFSIIFQGVWLMLMGFTLWTPSLISKGCFMHLEDGHKVVRCGGEAPLHRAKSLVN 240

Query: 241 LQFSWLLIALTIFALSFHLLLLKLYQQETPKNAAHYFSLMTDDEEKALEAAASTKLTPYD 300
           +QFSWLLIALTIFALS HL LLKLYQQ TPKN   Y SL+T + E+       T+     
Sbjct: 241 IQFSWLLIALTIFALSLHLFLLKLYQQ-TPKN-PQYISLITSEVEEETSKNIDTR----- 300

Query: 301 VASRSFIQMGHKFTE 316
             +RSFI+MGHKFTE
Sbjct: 301 --ARSFIEMGHKFTE 305

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT1G55230.11.5e-9459.09Family of unknown function (DUF716) [more]
AT1G55240.11.3e-8751.57Family of unknown function (DUF716) [more]
AT5G19870.11.3e-8553.87Family of unknown function (DUF716) [more]
AT1G49470.15.5e-4136.30Family of unknown function (DUF716) [more]
AT1G32120.16.0e-2730.23FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
Match NameE-valueIdentityDescription
Q9NWC53.1e-0427.23Transmembrane protein 45A OS=Homo sapiens OX=9606 GN=TMEM45A PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_038901896.12.0e-181100.00transmembrane protein 45B-like [Benincasa hispida][more]
XP_008449792.12.7e-15485.76PREDICTED: transmembrane protein 45A [Cucumis melo][more]
XP_004142158.11.8e-15084.15transmembrane protein 45A [Cucumis sativus] >KGN54083.1 hypothetical protein Csa... [more]
KAG7010234.11.2e-13880.19hypothetical protein SDJN02_27026, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_023513146.12.5e-13678.93transmembrane protein 45A-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A1S3BMV51.3e-15485.76transmembrane protein 45A OS=Cucumis melo OX=3656 GN=LOC103491576 PE=3 SV=1[more]
A0A0A0KX388.7e-15184.15Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G280600 PE=3 SV=1[more]
A0A6J1JGR52.1e-13678.62transmembrane protein 45A-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1FXU72.1e-13679.37transmembrane protein 45A-like OS=Cucurbita moschata OX=3662 GN=LOC111448740 PE=... [more]
A0A6J1JEQ64.6e-13679.05transmembrane protein 45A-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006904Protein of unknown function DUF716PFAMPF04819DUF716coord: 124..256
e-value: 3.0E-48
score: 163.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 343..360
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 333..360
NoneNo IPR availablePANTHERPTHR46285:SF3PROTEINASE INHIBITOR I4, SERPIN (DUF716)coord: 1..319
NoneNo IPR availablePANTHERPTHR46285PROTEINASE INHIBITOR I4, SERPIN (DUF716)-RELATEDcoord: 1..319

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi10M000334Bhi10M000334mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane