Bhi10G000309 (gene) Wax gourd (B227) v1

Overview


Name	Bhi10G000309
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Delta-12 fatty acid desaturase
Location	chr10: 8599815 .. 8600682 (+)
RNA-Seq Expression	Bhi10G000309
Synteny	Bhi10G000309

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

GCGTCATCTTCACCGCCCTCTGGGTCATCGCCCACGAATGCGGCCACCACGCCTTCAGCAACTACCAACTCCTCGACGACCTCCTCGGCTTCCTCCTCCACTCATCCCTTACTTCTCTTTCAAAATCAGCCACCGCCGCCACCACGCCAACAACGCATCCCTCGACCGCGACGAGGTCTTCGTCCCCAAGCCCAAGTCCAAAATCTCTTACTATTTCCACCACTTAACCAACCCACCGGCTAGGCTCCTCATCATTTTCATGACCCTCACGCTCGGCTGGCCATTGTACTTAGCCTTCAACAGGTCAGGCCGAGCCTACGACAGATTCACCAGCCATTACGATCCAAACAGCCCCATATTCAGCGAAAAGGAACGGCTTCAAGTTCAAATATCCATTGCGGGAGTTGTGACCATTTTGTACTTGCTCTACAAATTGGCAATTGTAAAAGGAATCACGTGGGTCGTTCGCATGTACTTACTGCCTTTAACGGTTATGAATGCGTTTGTGGTGTTGATTTTGTCGTTGCAACACACGCATCTTTCATTGCCGTATTATGATTCTAGTGAATGGGATTGGTTGAGAGGGAATTTGGTGACGGTTGATAGAGATTATGGGATGATTTTGAATAAAATGCTACATAATATAACCGACACACATGTCATTCATCATTTGTTTCCTACGATGCCCTATTATAACGCCGTGGAGGCGACGATGGCGGTGAAGCAAGTGTTGGGAGAATATTACCAGTTTGATGAGACGCCTATTTTGAAGGCCACGTGGAGGGAGTTTAGAGAGTGCATTTATGTAGAGGCAGATGATGAAGGTTCTAGCAGTAAAGGAGTTTTCTGGTTCCGTAACAAGCTTTGA

mRNA sequence

GCGTCATCTTCACCGCCCTCTGGGTCATCGCCCACGAATGCGGCCACCACGCCTTCAGCAACTACCAACTCCTCGACGACCTCCTCGGCTTCCTCCTCCACTCATCCCTTACTTCTCTTTCAAAATCAGCCACCGCCGCCACCACGCCAACAACGCATCCCTCGACCGCGACGAGGTCTTCGTCCCCAAGCCCAAGTCCAAAATCTCTTACTATTTCCACCACTTAACCAACCCACCGGCTAGGCTCCTCATCATTTTCATGACCCTCACGCTCGGCTGGCCATTGTACTTAGCCTTCAACAGGTCAGGCCGAGCCTACGACAGATTCACCAGCCATTACGATCCAAACAGCCCCATATTCAGCGAAAAGGAACGGCTTCAAGTTCAAATATCCATTGCGGGAGTTGTGACCATTTTGTACTTGCTCTACAAATTGGCAATTGTAAAAGGAATCACGTGGGTCGTTCGCATGTACTTACTGCCTTTAACGGTTATGAATGCGTTTGTGGTGTTGATTTTGTCGTTGCAACACACGCATCTTTCATTGCCGTATTATGATTCTAGTGAATGGGATTGGTTGAGAGGGAATTTGGTGACGGTTGATAGAGATTATGGGATGATTTTGAATAAAATGCTACATAATATAACCGACACACATGTCATTCATCATTTGTTTCCTACGATGCCCTATTATAACGCCGTGGAGGCGACGATGGCGGTGAAGCAAGTGTTGGGAGAATATTACCAGTTTGATGAGACGCCTATTTTGAAGGCCACGTGGAGGGAGTTTAGAGAGTGCATTTATGTAGAGGCAGATGATGAAGGTTCTAGCAGTAAAGGAGTTTTCTGGTTCCGTAACAAGCTTTGA

Coding sequence (CDS)

GCGTCATCTTCACCGCCCTCTGGGTCATCGCCCACGAATGCGGCCACCACGCCTTCAGCAACTACCAACTCCTCGACGACCTCCTCGGCTTCCTCCTCCACTCATCCCTTACTTCTCTTTCAAAATCAGCCACCGCCGCCACCACGCCAACAACGCATCCCTCGACCGCGACGAGGTCTTCGTCCCCAAGCCCAAGTCCAAAATCTCTTACTATTTCCACCACTTAACCAACCCACCGGCTAGGCTCCTCATCATTTTCATGACCCTCACGCTCGGCTGGCCATTGTACTTAGCCTTCAACAGGTCAGGCCGAGCCTACGACAGATTCACCAGCCATTACGATCCAAACAGCCCCATATTCAGCGAAAAGGAACGGCTTCAAGTTCAAATATCCATTGCGGGAGTTGTGACCATTTTGTACTTGCTCTACAAATTGGCAATTGTAAAAGGAATCACGTGGGTCGTTCGCATGTACTTACTGCCTTTAACGGTTATGAATGCGTTTGTGGTGTTGATTTTGTCGTTGCAACACACGCATCTTTCATTGCCGTATTATGATTCTAGTGAATGGGATTGGTTGAGAGGGAATTTGGTGACGGTTGATAGAGATTATGGGATGATTTTGAATAAAATGCTACATAATATAACCGACACACATGTCATTCATCATTTGTTTCCTACGATGCCCTATTATAACGCCGTGGAGGCGACGATGGCGGTGAAGCAAGTGTTGGGAGAATATTACCAGTTTGATGAGACGCCTATTTTGAAGGCCACGTGGAGGGAGTTTAGAGAGTGCATTTATGTAGAGGCAGATGATGAAGGTTCTAGCAGTAAAGGAGTTTTCTGGTTCCGTAACAAGCTTTGA

Protein sequence

RHLHRPLGHRPRMRPPRLQQLPTPRRPPRLPPPLIPYFSFKISHRRHHANNASLDRDEVFVPKPKSKISYYFHHLTNPPARLLIIFMTLTLGWPLYLAFNRSGRAYDRFTSHYDPNSPIFSEKERLQVQISIAGVVTILYLLYKLAIVKGITWVVRMYLLPLTVMNAFVVLILSLQHTHLSLPYYDSSEWDWLRGNLVTVDRDYGMILNKMLHNITDTHVIHHLFPTMPYYNAVEATMAVKQVLGEYYQFDETPILKATWREFRECIYVEADDEGSSSKGVFWFRNKL

Homology

[Close]

BLAST of Bhi10G000309 vs. TAIR 10
Match: AT3G12120.1 (fatty acid desaturase 2 )

HSP 1 Score: 327.4 bits (838), Expect = 1.2e-89
Identity = 154/255 (60.39%), Postives = 198/255 (77.65%), Query Frame = 0


Query: 34  LIPYFSFKISHRRHHANNASLDRDEVFVPKPKSKISYYFHHLTNPPARLLIIFMTLTLGW 93
           L+PYFS+K SHRRHH+N  SL+RDEVFVPK KS I +Y  +L NP  R++++ +   LGW
Sbjct: 131 LVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYGKYLNNPLGRIMMLTVQFVLGW 190

Query: 94  PLYLAFNRSGRAYDRFTSHYDPNSPIFSEKERLQVQISIAGVVTILYLLYKLAIVKGITW 153
           PLYLAFN SGR YD F  H+ PN+PI++++ERLQ+ +S AG++ + + LY+ A  +G+  
Sbjct: 191 PLYLAFNVSGRPYDGFACHFFPNAPIYNDRERLQIYLSDAGILAVCFGLYRYAAAQGMAS 250

Query: 154 VVRMYLLPLTVMNAFVVLILSLQHTHLSLPYYDSSEWDWLRGNLVTVDRDYGMILNKMLH 213
           ++ +Y +PL ++NAF+VLI  LQHTH SLP+YDSSEWDWLRG L TVDRDYG ILNK+ H
Sbjct: 251 MICLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRDYG-ILNKVFH 310

Query: 214 NITDTHVIHHLFPTMPYYNAVEATMAVKQVLGEYYQFDETPILKATWREFRECIYVEADD 273
           NITDTHV HHLF TMP+YNA+EAT A+K +LG+YYQFD TP   A +RE +ECIYVE D 
Sbjct: 311 NITDTHVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYREAKECIYVEPDR 370

Query: 274 EGSSSKGVFWFRNKL 289
           EG   KGV+W+ NKL
Sbjct: 371 EG-DKKGVYWYNNKL 383

[Close]

BLAST of Bhi10G000309 vs. TAIR 10
Match: AT3G12120.2 (fatty acid desaturase 2 )

HSP 1 Score: 327.4 bits (838), Expect = 1.2e-89
Identity = 154/255 (60.39%), Postives = 198/255 (77.65%), Query Frame = 0


Query: 34  LIPYFSFKISHRRHHANNASLDRDEVFVPKPKSKISYYFHHLTNPPARLLIIFMTLTLGW 93
           L+PYFS+K SHRRHH+N  SL+RDEVFVPK KS I +Y  +L NP  R++++ +   LGW
Sbjct: 131 LVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYGKYLNNPLGRIMMLTVQFVLGW 190

Query: 94  PLYLAFNRSGRAYDRFTSHYDPNSPIFSEKERLQVQISIAGVVTILYLLYKLAIVKGITW 153
           PLYLAFN SGR YD F  H+ PN+PI++++ERLQ+ +S AG++ + + LY+ A  +G+  
Sbjct: 191 PLYLAFNVSGRPYDGFACHFFPNAPIYNDRERLQIYLSDAGILAVCFGLYRYAAAQGMAS 250

Query: 154 VVRMYLLPLTVMNAFVVLILSLQHTHLSLPYYDSSEWDWLRGNLVTVDRDYGMILNKMLH 213
           ++ +Y +PL ++NAF+VLI  LQHTH SLP+YDSSEWDWLRG L TVDRDYG ILNK+ H
Sbjct: 251 MICLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRDYG-ILNKVFH 310

Query: 214 NITDTHVIHHLFPTMPYYNAVEATMAVKQVLGEYYQFDETPILKATWREFRECIYVEADD 273
           NITDTHV HHLF TMP+YNA+EAT A+K +LG+YYQFD TP   A +RE +ECIYVE D 
Sbjct: 311 NITDTHVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYREAKECIYVEPDR 370

Query: 274 EGSSSKGVFWFRNKL 289
           EG   KGV+W+ NKL
Sbjct: 371 EG-DKKGVYWYNNKL 383

[Close]

BLAST of Bhi10G000309 vs. TAIR 10
Match: AT2G29980.1 (fatty acid desaturase 3 )

HSP 1 Score: 131.7 bits (330), Expect = 9.3e-31
Identity = 82/223 (36.77%), Postives = 124/223 (55.61%), Query Frame = 0


Query: 34  LIPYFSFKISHRRHHANNASLDRDEVFVPKPKSKISYYFHHLTNPPARLLIIFMTL-TLG 93
           L+PY  ++ISHR HH N+  ++ DE +VP P+ ++     H T    R+L   + L  L 
Sbjct: 127 LVPYHGWRISHRTHHQNHGHVENDESWVPLPE-RVYKKLPHST----RMLRYTVPLPMLA 186

Query: 94  WPLYLAFNRSGRAYDRFTSHYDPNSPIFSEKERLQVQISIAGVVTILYLLYKLAIVKGIT 153
           +PLYL +   G+      SH++P S +F+  ER  +  S      +   L  L+ V G  
Sbjct: 187 YPLYLCYRSPGKE----GSHFNPYSSLFAPSERKLIATSTTCWSIMFVSLIALSFVFGPL 246

Query: 154 WVVRMYLLPLTVMNAFVVLILSLQH--THLSLPYYDSSEWDWLRGNLVTVDRDYGMILNK 213
            V+++Y +P  +   ++  +  L H      LP+Y   EW +LRG L T+DRDYG+  N 
Sbjct: 247 AVLKVYGVPYIIFVMWLDAVTYLHHHGHDEKLPWYRGKEWSYLRGGLTTIDRDYGIFNN- 306

Query: 214 MLHNITDTHVIHHLFPTMPYYNAVEATMAVKQVLGEYYQFDET 254
            +H+   THVIHHLFP +P+Y+ V+AT A K VLG YY+  +T
Sbjct: 307 -IHHDIGTHVIHHLFPQIPHYHLVDATKAAKHVLGRYYREPKT 338

[Close]

BLAST of Bhi10G000309 vs. TAIR 10
Match: AT5G05580.1 (fatty acid desaturase 8 )

HSP 1 Score: 131.3 bits (329), Expect = 1.2e-30
Identity = 83/218 (38.07%), Postives = 119/218 (54.59%), Query Frame = 0


Query: 34  LIPYFSFKISHRRHHANNASLDRDEVFVPKPKSKISYYFHHLTNPPARLLIIFMTLTLGW 93
           L+PY  ++ISHR HH N+  ++ DE + P P+S     + +L               L +
Sbjct: 182 LVPYHGWRISHRTHHQNHGHVENDESWHPLPES----IYKNLEKTTQMFRFTLPFPMLAY 241

Query: 94  PLYLAFNRSGRAYDRFTSHYDPNSPIFSEKERLQVQISIAGVVTILYLLYKLAIVKGITW 153
           P YL +NRS     +  SHY P+S +F  KE+  V  S A    +  LL  L  V G   
Sbjct: 242 PFYL-WNRSP---GKQGSHYHPDSDLFLPKEKKDVLTSTACWTAMAALLVCLNFVMGPIQ 301

Query: 154 VVRMYLLPLTVMNAFVVLILSLQH--THLSLPYYDSSEWDWLRGNLVTVDRDYGMILNKM 213
           ++++Y +P  +   ++  +  L H      LP+Y   EW +LRG L T+DRDYG I N  
Sbjct: 302 MLKLYGIPYWIFVMWLDFVTYLHHHGHEDKLPWYRGKEWSYLRGGLTTLDRDYGWINN-- 361

Query: 214 LHNITDTHVIHHLFPTMPYYNAVEATMAVKQVLGEYYQ 250
           +H+   THVIHHLFP +P+Y+ VEAT A K VLG+YY+
Sbjct: 362 IHHDIGTHVIHHLFPQIPHYHLVEATEAAKPVLGKYYR 389

[Close]

BLAST of Bhi10G000309 vs. TAIR 10
Match: AT3G11170.1 (fatty acid desaturase 7 )

HSP 1 Score: 131.0 bits (328), Expect = 1.6e-30
Identity = 82/219 (37.44%), Postives = 119/219 (54.34%), Query Frame = 0


Query: 34  LIPYFSFKISHRRHHANNASLDRDEVFVPKPKSKISYYFHHLTNPPARLLIIFMTLT-LG 93
           L+PY  ++ISHR HH N+  ++ DE + P     +S   ++  + P R     + L  L 
Sbjct: 189 LVPYHGWRISHRTHHQNHGHVENDESWHP-----MSEKIYNTLDKPTRFFRFTLPLVMLA 248

Query: 94  WPLYLAFNRSGRAYDRFTSHYDPNSPIFSEKERLQVQISIAGVVTILYLLYKLAIVKGIT 153
           +P YL     G+      SHY P+S +F  KER  V  S A    +  LL  L    G  
Sbjct: 249 YPFYLWARSPGKK----GSHYHPDSDLFLPKERKDVLTSTACWTAMAALLVCLNFTIGPI 308

Query: 154 WVVRMYLLPLTVMNAFVVLILSLQH--THLSLPYYDSSEWDWLRGNLVTVDRDYGMILNK 213
            ++++Y +P  +   ++  +  L H      LP+Y   EW +LRG L T+DRDYG+I N 
Sbjct: 309 QMLKLYGIPYWINVMWLDFVTYLHHHGHEDKLPWYRGKEWSYLRGGLTTLDRDYGLINN- 368

Query: 214 MLHNITDTHVIHHLFPTMPYYNAVEATMAVKQVLGEYYQ 250
            +H+   THVIHHLFP +P+Y+ VEAT A K VLG+YY+
Sbjct: 369 -IHHDIGTHVIHHLFPQIPHYHLVEATEAAKPVLGKYYR 396

[Close]

BLAST of Bhi10G000309 vs. ExPASy Swiss-Prot
Match: Q84UC0 (Bifunctional fatty acid conjugase/Delta(12)-oleate desaturase OS=Trichosanthes kirilowii OX=3677 GN=FAC PE=2 SV=1)

HSP 1 Score: 426.8 bits (1096), Expect = 2.0e-118
Identity = 205/258 (79.46%), Postives = 230/258 (89.15%), Query Frame = 0


Query: 34  LIPYFSFKISHRRHHANNASLDRDEVFVPKPKSKISYYFHHLTNPPARLLIIFMTLTLGW 93
           LIPYFSFKISHRRHH+N ASL+RDEVFVPKPK+K+ +YF HLTNPPAR+LIIF+TLTLGW
Sbjct: 131 LIPYFSFKISHRRHHSNTASLERDEVFVPKPKAKMPWYFKHLTNPPARVLIIFITLTLGW 190

Query: 94  PLYLAFNRSGRAYDRFTSHYDPNSPIFSEKERLQVQISIAGVVTILYLLYKLAIVKGITW 153
           P+YLAFN SGR Y+RFTSH+DPNSPIFSE E LQV IS AG+V + YLLYKLA  KGI W
Sbjct: 191 PMYLAFNISGRFYERFTSHFDPNSPIFSENEWLQVHISNAGIVAVWYLLYKLAAAKGIAW 250

Query: 154 VVRMYLLPLTVMNAFVVLILSLQHTHLSLPYYDSSEWDWLRGNLVTVDRDYGMILNKMLH 213
           V+RMY++P+T+MNAFVVLI SLQHTH S PYYDS+EW+WLRGNLVT+DRDYG ILNK+ H
Sbjct: 251 VIRMYVVPVTIMNAFVVLITSLQHTHPSFPYYDSTEWNWLRGNLVTLDRDYG-ILNKVFH 310

Query: 214 NITDTHVIHHLFPTMPYYNAVEATMAVKQVLGEYYQFDETPILKATWREFRECIYVEAD- 273
           NITDTHV+HHLFP+MP+YNA+EAT AVKQVLGEYY FD TPI KA WREFRECIYVE D 
Sbjct: 311 NITDTHVVHHLFPSMPHYNAMEATRAVKQVLGEYYHFDGTPIFKAAWREFRECIYVEPDN 370

Query: 274 DEG--SSSKGVFWFRNKL 289
           DEG  SSSKGVFWFRNKL
Sbjct: 371 DEGASSSSKGVFWFRNKL 387

[Close]

BLAST of Bhi10G000309 vs. ExPASy Swiss-Prot
Match: Q9SP61 (Delta(12) acyl-lipid conjugase (11E,13E-forming) OS=Momordica charantia OX=3673 GN=FADX PE=1 SV=1)

HSP 1 Score: 396.7 bits (1018), Expect = 2.2e-109
Identity = 182/259 (70.27%), Postives = 221/259 (85.33%), Query Frame = 0


Query: 35  IPYFSFKISHRRHHANNASLDRDEVFVPKPKSKISYYFHHLTNPPARLLIIFMTLTLGWP 94
           +PYFSFKISHRRHH+N +S+DRDEVFVPKPK+K+ +YF +LTNPPAR+ IIF+TLTLGWP
Sbjct: 141 VPYFSFKISHRRHHSNTSSVDRDEVFVPKPKAKMPWYFKYLTNPPARVFIIFITLTLGWP 200

Query: 95  LYLAFNRSGRAYDRFTSHYDPNSPIFSEKERLQVQISIAGVVTILYLLYKLAIVKGITWV 154
           +YL FN SGR Y RFTSH+DPNSPIFS KER+ V IS AG+V   YLLY++A+ KG+ W+
Sbjct: 201 MYLTFNISGRYYGRFTSHFDPNSPIFSPKERVLVHISNAGLVATGYLLYRIAMAKGVGWL 260

Query: 155 VRMYLLPLTVMNAFVVLILSLQHTHLSLPYYDSSEWDWLRGNLVTVDRDYGMILNKMLHN 214
           +R+Y +PL V+NA VVLI +LQHTH S PYYDS+EWDWLRGNLVTVDRDYG I+N++ H+
Sbjct: 261 IRLYGVPLIVLNACVVLITALQHTHPSFPYYDSTEWDWLRGNLVTVDRDYGPIMNRVFHH 320

Query: 215 ITDTHVIHHLFPTMPYYNAVEATMAVKQVLGEYYQFDETPILKATWREFRECIYVEADDE 274
           ITDTHV+HHLFP+MP+YN  EAT+A K++LGEYYQFD TPI KA WREFREC+YVE D++
Sbjct: 321 ITDTHVVHHLFPSMPHYNGKEATVAAKRILGEYYQFDGTPIWKAAWREFRECVYVEPDED 380

Query: 275 -----GSSSKGVFWFRNKL 289
                GSSSKGVFW+ NKL
Sbjct: 381 DGATSGSSSKGVFWYHNKL 399

[Close]

BLAST of Bhi10G000309 vs. ExPASy Swiss-Prot
Match: P48631 (Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2 OS=Glycine max OX=3847 GN=FAD2-2 PE=2 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 1.5e-97
Identity = 169/255 (66.27%), Postives = 206/255 (80.78%), Query Frame = 0


Query: 34  LIPYFSFKISHRRHHANNASLDRDEVFVPKPKSKISYYFHHLTNPPARLLIIFMTLTLGW 93
           L+PYFS+K SHRRHH+N  SL+RDEVFVPK KS I +Y  +L NPP R+L + +TLTLGW
Sbjct: 131 LVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSCIKWYSKYLNNPPGRVLTLAVTLTLGW 190

Query: 94  PLYLAFNRSGRAYDRFTSHYDPNSPIFSEKERLQVQISIAGVVTILYLLYKLAIVKGITW 153
           PLYLA N SGR YDRF  HYDP  PI+S++ERLQ+ IS AGV+ ++Y L++LA+ KG+ W
Sbjct: 191 PLYLALNVSGRPYDRFACHYDPYGPIYSDRERLQIYISDAGVLAVVYGLFRLAMAKGLAW 250

Query: 154 VVRMYLLPLTVMNAFVVLILSLQHTHLSLPYYDSSEWDWLRGNLVTVDRDYGMILNKMLH 213
           VV +Y +PL V+N F+VLI  LQHTH +LP+Y SSEWDWLRG L TVDRDYG ILNK+ H
Sbjct: 251 VVCVYGVPLLVVNGFLVLITFLQHTHPALPHYTSSEWDWLRGALATVDRDYG-ILNKVFH 310

Query: 214 NITDTHVIHHLFPTMPYYNAVEATMAVKQVLGEYYQFDETPILKATWREFRECIYVEADD 273
           NITDTHV HHLF TMP+Y+A+EAT A+K +LGEYY+FDETP +KA WRE RECIYVE  D
Sbjct: 311 NITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYRFDETPFVKAMWREARECIYVE-PD 370

Query: 274 EGSSSKGVFWFRNKL 289
           + + SKGVFW+ NKL
Sbjct: 371 QSTESKGVFWYNNKL 383

[Close]

BLAST of Bhi10G000309 vs. ExPASy Swiss-Prot
Match: Q84UB9 (Delta(12)-oleate desaturase OS=Trichosanthes kirilowii OX=3677 GN=FAD2 PE=2 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 1.9e-97
Identity = 162/255 (63.53%), Postives = 205/255 (80.39%), Query Frame = 0


Query: 34  LIPYFSFKISHRRHHANNASLDRDEVFVPKPKSKISYYFHHLTNPPARLLIIFMTLTLGW 93
           L+PYFS+K SHRRHH+N  SL+RDEVFVPKPKS+I +Y  +  NPP RL+ +  TLTLGW
Sbjct: 116 LVPYFSWKYSHRRHHSNTGSLERDEVFVPKPKSQIPWYLKYFNNPPGRLISLIGTLTLGW 175

Query: 94  PLYLAFNRSGRAYDRFTSHYDPNSPIFSEKERLQVQISIAGVVTILYLLYKLAIVKGITW 153
           PLYLAFN SGR YDRF  HYDP SPIF+++E +QV IS AG++ + ++LY++ + KG  W
Sbjct: 176 PLYLAFNMSGRPYDRFACHYDPYSPIFTKREWIQVYISDAGILGMAFMLYRIGVEKGSFW 235

Query: 154 VVRMYLLPLTVMNAFVVLILSLQHTHLSLPYYDSSEWDWLRGNLVTVDRDYGMILNKMLH 213
           V+R+Y +PL ++N F+VLI  LQHTH +LP+Y+SSEWDWLRG L TVDRDYG +LN++ H
Sbjct: 236 VMRIYGIPLVIVNGFLVLITYLQHTHPALPHYESSEWDWLRGALATVDRDYG-VLNRVFH 295

Query: 214 NITDTHVIHHLFPTMPYYNAVEATMAVKQVLGEYYQFDETPILKATWREFRECIYVEADD 273
           NITDTHV+HHLF TMP+Y+A EAT AVK VLGEYY+ D TP+LKA WRE +EC+YVE D+
Sbjct: 296 NITDTHVVHHLFSTMPHYHAKEATEAVKPVLGEYYRLDRTPVLKAIWREAKECVYVEPDE 355

Query: 274 EGSSSKGVFWFRNKL 289
           +  S KGV W+R KL
Sbjct: 356 DADSDKGVVWYRKKL 369

[Close]

BLAST of Bhi10G000309 vs. ExPASy Swiss-Prot
Match: Q8GZC3 (Delta(12)-fatty-acid desaturase FAD2 OS=Vernicia fordii OX=73154 GN=FAD2 PE=1 SV=1)

HSP 1 Score: 353.6 bits (906), Expect = 2.1e-96
Identity = 165/254 (64.96%), Postives = 203/254 (79.92%), Query Frame = 0


Query: 34  LIPYFSFKISHRRHHANNASLDRDEVFVPKPKSKISYYFHHLTNPPARLLIIFMTLTLGW 93
           L+PYFS+K SHRRHH+N ASL+RDEVFVPK KS I ++  +L NPP RL  + +TLTLGW
Sbjct: 131 LVPYFSWKHSHRRHHSNTASLERDEVFVPKKKSSIRWFSKYLNNPPGRLFTLTITLTLGW 190

Query: 94  PLYLAFNRSGRAYDRFTSHYDPNSPIFSEKERLQVQISIAGVVTILYLLYKLAIVKGITW 153
           PLYLAFN SGR YDRF  HYDP  PI++++ER ++ IS AGV+ + + LY+LA  KG+ W
Sbjct: 191 PLYLAFNVSGRPYDRFACHYDPYGPIYTDRERTEIYISDAGVLAVTFGLYRLAAAKGLAW 250

Query: 154 VVRMYLLPLTVMNAFVVLILSLQHTHLSLPYYDSSEWDWLRGNLVTVDRDYGMILNKMLH 213
           V+ +Y +PL ++NAF+V+I  LQHTH S+P+YDSSEWDWLRG L TVDRDYG ILNK+ H
Sbjct: 251 VICVYGVPLLIVNAFLVMITYLQHTHPSIPHYDSSEWDWLRGALATVDRDYG-ILNKVFH 310

Query: 214 NITDTHVIHHLFPTMPYYNAVEATMAVKQVLGEYYQFDETPILKATWREFRECIYVEADD 273
           NITDTHV HHLF TMP+Y+A+EAT A+K +LGEYYQFD TP  KA WRE +ECIYVEADD
Sbjct: 311 NITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYQFDGTPFYKAMWREAKECIYVEADD 370

Query: 274 EGSSSKGVFWFRNK 288
            G  SKGV+W+  K
Sbjct: 371 -GDESKGVYWYNKK 382

[Close]

BLAST of Bhi10G000309 vs. NCBI nr
Match: XP_038902275.1 (LOW QUALITY PROTEIN: bifunctional fatty acid conjugase/Delta(12)-oleate desaturase-like [Benincasa hispida])

HSP 1 Score: 523.1 bits (1346), Expect = 1.5e-144
Identity = 254/254 (100.00%), Postives = 254/254 (100.00%), Query Frame = 0


Query: 35  IPYFSFKISHRRHHANNASLDRDEVFVPKPKSKISYYFHHLTNPPARLLIIFMTLTLGWP 94
           IPYFSFKISHRRHHANNASLDRDEVFVPKPKSKISYYFHHLTNPPARLLIIFMTLTLGWP
Sbjct: 120 IPYFSFKISHRRHHANNASLDRDEVFVPKPKSKISYYFHHLTNPPARLLIIFMTLTLGWP 179

Query: 95  LYLAFNRSGRAYDRFTSHYDPNSPIFSEKERLQVQISIAGVVTILYLLYKLAIVKGITWV 154
           LYLAFNRSGRAYDRFTSHYDPNSPIFSEKERLQVQISIAGVVTILYLLYKLAIVKGITWV
Sbjct: 180 LYLAFNRSGRAYDRFTSHYDPNSPIFSEKERLQVQISIAGVVTILYLLYKLAIVKGITWV 239

Query: 155 VRMYLLPLTVMNAFVVLILSLQHTHLSLPYYDSSEWDWLRGNLVTVDRDYGMILNKMLHN 214
           VRMYLLPLTVMNAFVVLILSLQHTHLSLPYYDSSEWDWLRGNLVTVDRDYGMILNKMLHN
Sbjct: 240 VRMYLLPLTVMNAFVVLILSLQHTHLSLPYYDSSEWDWLRGNLVTVDRDYGMILNKMLHN 299

Query: 215 ITDTHVIHHLFPTMPYYNAVEATMAVKQVLGEYYQFDETPILKATWREFRECIYVEADDE 274
           ITDTHVIHHLFPTMPYYNAVEATMAVKQVLGEYYQFDETPILKATWREFRECIYVEADDE
Sbjct: 300 ITDTHVIHHLFPTMPYYNAVEATMAVKQVLGEYYQFDETPILKATWREFRECIYVEADDE 359

Query: 275 GSSSKGVFWFRNKL 289
           GSSSKGVFWFRNKL
Sbjct: 360 GSSSKGVFWFRNKL 373

[Close]

BLAST of Bhi10G000309 vs. NCBI nr
Match: XP_008449949.1 (PREDICTED: bifunctional fatty acid conjugase/Delta(12)-oleate desaturase-like [Cucumis melo] >KAA0041359.1 bifunctional fatty acid conjugase/Delta(12)-oleate desaturase-like [Cucumis melo var. makuwa])

HSP 1 Score: 458.8 bits (1179), Expect = 3.6e-125
Identity = 222/261 (85.06%), Postives = 235/261 (90.04%), Query Frame = 0


Query: 34  LIPYFSFKISHRRHHANNASLDRDEVFVPKPKSKISYYFHHLTNPPARLLIIFMTLTLGW 93
           LIPYFSFKISHRRHHAN ASL+RDEVFVPK KSKI +YF HLTNPPARL II MTLTLGW
Sbjct: 126 LIPYFSFKISHRRHHANTASLNRDEVFVPKLKSKIPWYFKHLTNPPARLFIIVMTLTLGW 185

Query: 94  PLYLAFNRSGRAYDRFTSHYDPNSPIFSEKERLQVQISIAGVVTILYLLYKLAIVKGITW 153
           P+YLAFN SGR YDRFTSHYDPNSPIF+EKERLQVQIS AGV+TILYLLYKLA+ KGITW
Sbjct: 186 PMYLAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKLAVTKGITW 245

Query: 154 VVRMYLLPLTVMNAFVVLILSLQHTHLSLPYYDSSEWDWLRGNLVTVDRDYGMILNKMLH 213
           V+R+YLLPLTVMN FVVLI SLQHTH SLPYYDSS+WDWLRGNLVTVDRDYG ILNKMLH
Sbjct: 246 VIRLYLLPLTVMNVFVVLISSLQHTHPSLPYYDSSQWDWLRGNLVTVDRDYGKILNKMLH 305

Query: 214 NITDTHVIHHLFPTMPYYNAVEATMAVKQVLGEYYQFDETPILKATWREFRECIYVEADD 273
           NITDTHVIHHLFP+MP+YNA EAT AVK+VLGEYYQFDETPILKA WREFREC+YVE DD
Sbjct: 306 NITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFRECVYVEEDD 365

Query: 274 ------EGSSSKGVFWFRNKL 289
                 EG  SKGVFWFRNKL
Sbjct: 366 NEEGDSEGFRSKGVFWFRNKL 386

[Close]

BLAST of Bhi10G000309 vs. NCBI nr
Match: TYK21657.1 (bifunctional fatty acid conjugase/Delta(12)-oleate desaturase-like [Cucumis melo var. makuwa])

HSP 1 Score: 457.6 bits (1176), Expect = 7.9e-125
Identity = 220/261 (84.29%), Postives = 234/261 (89.66%), Query Frame = 0


Query: 34  LIPYFSFKISHRRHHANNASLDRDEVFVPKPKSKISYYFHHLTNPPARLLIIFMTLTLGW 93
           LIPYFSFKISHRRHHAN ASL+RDEVFVPKPKSKI +YF HLTNPPARL II MTLTLGW
Sbjct: 126 LIPYFSFKISHRRHHANTASLNRDEVFVPKPKSKIPWYFKHLTNPPARLFIIVMTLTLGW 185

Query: 94  PLYLAFNRSGRAYDRFTSHYDPNSPIFSEKERLQVQISIAGVVTILYLLYKLAIVKGITW 153
           P+Y+AFN SGR YDRFTSHYDPNSPIF+EKERLQVQIS AGV+TILYLLYK A+ KGI W
Sbjct: 186 PMYIAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKFAVTKGIMW 245

Query: 154 VVRMYLLPLTVMNAFVVLILSLQHTHLSLPYYDSSEWDWLRGNLVTVDRDYGMILNKMLH 213
           V+R+YLLPLTVMN FVVLI SLQHTH SLPYYDSS+WDWLRGNLVTVDRDYG ILNKMLH
Sbjct: 246 VIRIYLLPLTVMNVFVVLISSLQHTHPSLPYYDSSQWDWLRGNLVTVDRDYGKILNKMLH 305

Query: 214 NITDTHVIHHLFPTMPYYNAVEATMAVKQVLGEYYQFDETPILKATWREFRECIYVEADD 273
           NITDTHVIHHLFP+MP+YNA EAT AVK+VLGEYYQFDETPILKA WREFREC+YVE DD
Sbjct: 306 NITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFRECVYVEEDD 365

Query: 274 ------EGSSSKGVFWFRNKL 289
                 EG  SKGVFWFRNKL
Sbjct: 366 NEEGDSEGFRSKGVFWFRNKL 386

[Close]

BLAST of Bhi10G000309 vs. NCBI nr
Match: XP_011653570.2 (bifunctional fatty acid conjugase/Delta(12)-oleate desaturase [Cucumis sativus])

HSP 1 Score: 457.6 bits (1176), Expect = 7.9e-125
Identity = 222/261 (85.06%), Postives = 235/261 (90.04%), Query Frame = 0


Query: 34  LIPYFSFKISHRRHHANNASLDRDEVFVPKPKSKISYYFHHLTNPPARLLIIFMTLTLGW 93
           LIPYFSFKISHRRHHAN ASL RDEVFVPKPKSKI +YF HLTNPPARL II MTLTLGW
Sbjct: 158 LIPYFSFKISHRRHHANTASLHRDEVFVPKPKSKIPWYFKHLTNPPARLFIIVMTLTLGW 217

Query: 94  PLYLAFNRSGRAYDRFTSHYDPNSPIFSEKERLQVQISIAGVVTILYLLYKLAIVKGITW 153
           P+Y+AFN SGR YDRFTSHYDPNSPIF+EKERLQVQIS AGV+TILYLLYKLA+ KGITW
Sbjct: 218 PMYIAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKLAVTKGITW 277

Query: 154 VVRMYLLPLTVMNAFVVLILSLQHTHLSLPYYDSSEWDWLRGNLVTVDRDYGMILNKMLH 213
           V+R+YLLPLTVMN FVVLI  LQHTH SLPYYDSS+WDWLRGNLVTVDRDYG ILNKMLH
Sbjct: 278 VIRIYLLPLTVMNVFVVLISCLQHTHSSLPYYDSSQWDWLRGNLVTVDRDYGKILNKMLH 337

Query: 214 NITDTHVIHHLFPTMPYYNAVEATMAVKQVLGEYYQFDETPILKATWREFRECIYVEADD 273
           NITDTHVIHHLFP+MP+YNA EAT AVK+VLGEYYQFDETPILKA WREFREC+YVE DD
Sbjct: 338 NITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFRECVYVEEDD 397

Query: 274 --EGSS----SKGVFWFRNKL 289
             EG S    SKGVFWFRNKL
Sbjct: 398 HEEGDSEAFRSKGVFWFRNKL 418

[Close]

BLAST of Bhi10G000309 vs. NCBI nr
Match: Q84UC0.1 (RecName: Full=Bifunctional fatty acid conjugase/Delta(12)-oleate desaturase; Short=TkFAC; AltName: Full=Delta(12)-oleate desaturase; AltName: Full=Linoleoyl-lipid Delta(12) conjugase (11E,13Z-forming) [Trichosanthes kirilowii] >AAO37751.1 fatty acid conjugase [Trichosanthes kirilowii])

HSP 1 Score: 426.8 bits (1096), Expect = 1.5e-115
Identity = 205/258 (79.46%), Postives = 230/258 (89.15%), Query Frame = 0


Query: 34  LIPYFSFKISHRRHHANNASLDRDEVFVPKPKSKISYYFHHLTNPPARLLIIFMTLTLGW 93
           LIPYFSFKISHRRHH+N ASL+RDEVFVPKPK+K+ +YF HLTNPPAR+LIIF+TLTLGW
Sbjct: 131 LIPYFSFKISHRRHHSNTASLERDEVFVPKPKAKMPWYFKHLTNPPARVLIIFITLTLGW 190

Query: 94  PLYLAFNRSGRAYDRFTSHYDPNSPIFSEKERLQVQISIAGVVTILYLLYKLAIVKGITW 153
           P+YLAFN SGR Y+RFTSH+DPNSPIFSE E LQV IS AG+V + YLLYKLA  KGI W
Sbjct: 191 PMYLAFNISGRFYERFTSHFDPNSPIFSENEWLQVHISNAGIVAVWYLLYKLAAAKGIAW 250

Query: 154 VVRMYLLPLTVMNAFVVLILSLQHTHLSLPYYDSSEWDWLRGNLVTVDRDYGMILNKMLH 213
           V+RMY++P+T+MNAFVVLI SLQHTH S PYYDS+EW+WLRGNLVT+DRDYG ILNK+ H
Sbjct: 251 VIRMYVVPVTIMNAFVVLITSLQHTHPSFPYYDSTEWNWLRGNLVTLDRDYG-ILNKVFH 310

Query: 214 NITDTHVIHHLFPTMPYYNAVEATMAVKQVLGEYYQFDETPILKATWREFRECIYVEAD- 273
           NITDTHV+HHLFP+MP+YNA+EAT AVKQVLGEYY FD TPI KA WREFRECIYVE D 
Sbjct: 311 NITDTHVVHHLFPSMPHYNAMEATRAVKQVLGEYYHFDGTPIFKAAWREFRECIYVEPDN 370

Query: 274 DEG--SSSKGVFWFRNKL 289
           DEG  SSSKGVFWFRNKL
Sbjct: 371 DEGASSSSKGVFWFRNKL 387

[Close]

BLAST of Bhi10G000309 vs. ExPASy TrEMBL
Match: A0A5A7TJ17 (Bifunctional fatty acid conjugase/Delta(12)-oleate desaturase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00030 PE=3 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 1.7e-125
Identity = 222/261 (85.06%), Postives = 235/261 (90.04%), Query Frame = 0


Query: 34  LIPYFSFKISHRRHHANNASLDRDEVFVPKPKSKISYYFHHLTNPPARLLIIFMTLTLGW 93
           LIPYFSFKISHRRHHAN ASL+RDEVFVPK KSKI +YF HLTNPPARL II MTLTLGW
Sbjct: 126 LIPYFSFKISHRRHHANTASLNRDEVFVPKLKSKIPWYFKHLTNPPARLFIIVMTLTLGW 185

Query: 94  PLYLAFNRSGRAYDRFTSHYDPNSPIFSEKERLQVQISIAGVVTILYLLYKLAIVKGITW 153
           P+YLAFN SGR YDRFTSHYDPNSPIF+EKERLQVQIS AGV+TILYLLYKLA+ KGITW
Sbjct: 186 PMYLAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKLAVTKGITW 245

Query: 154 VVRMYLLPLTVMNAFVVLILSLQHTHLSLPYYDSSEWDWLRGNLVTVDRDYGMILNKMLH 213
           V+R+YLLPLTVMN FVVLI SLQHTH SLPYYDSS+WDWLRGNLVTVDRDYG ILNKMLH
Sbjct: 246 VIRLYLLPLTVMNVFVVLISSLQHTHPSLPYYDSSQWDWLRGNLVTVDRDYGKILNKMLH 305

Query: 214 NITDTHVIHHLFPTMPYYNAVEATMAVKQVLGEYYQFDETPILKATWREFRECIYVEADD 273
           NITDTHVIHHLFP+MP+YNA EAT AVK+VLGEYYQFDETPILKA WREFREC+YVE DD
Sbjct: 306 NITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFRECVYVEEDD 365

Query: 274 ------EGSSSKGVFWFRNKL 289
                 EG  SKGVFWFRNKL
Sbjct: 366 NEEGDSEGFRSKGVFWFRNKL 386

[Close]

BLAST of Bhi10G000309 vs. ExPASy TrEMBL
Match: A0A1S3BMK7 (bifunctional fatty acid conjugase/Delta(12)-oleate desaturase-like OS=Cucumis melo OX=3656 GN=LOC103491678 PE=3 SV=1)

HSP 1 Score: 458.8 bits (1179), Expect = 1.7e-125
Identity = 222/261 (85.06%), Postives = 235/261 (90.04%), Query Frame = 0


Query: 34  LIPYFSFKISHRRHHANNASLDRDEVFVPKPKSKISYYFHHLTNPPARLLIIFMTLTLGW 93
           LIPYFSFKISHRRHHAN ASL+RDEVFVPK KSKI +YF HLTNPPARL II MTLTLGW
Sbjct: 126 LIPYFSFKISHRRHHANTASLNRDEVFVPKLKSKIPWYFKHLTNPPARLFIIVMTLTLGW 185

Query: 94  PLYLAFNRSGRAYDRFTSHYDPNSPIFSEKERLQVQISIAGVVTILYLLYKLAIVKGITW 153
           P+YLAFN SGR YDRFTSHYDPNSPIF+EKERLQVQIS AGV+TILYLLYKLA+ KGITW
Sbjct: 186 PMYLAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKLAVTKGITW 245

Query: 154 VVRMYLLPLTVMNAFVVLILSLQHTHLSLPYYDSSEWDWLRGNLVTVDRDYGMILNKMLH 213
           V+R+YLLPLTVMN FVVLI SLQHTH SLPYYDSS+WDWLRGNLVTVDRDYG ILNKMLH
Sbjct: 246 VIRLYLLPLTVMNVFVVLISSLQHTHPSLPYYDSSQWDWLRGNLVTVDRDYGKILNKMLH 305

Query: 214 NITDTHVIHHLFPTMPYYNAVEATMAVKQVLGEYYQFDETPILKATWREFRECIYVEADD 273
           NITDTHVIHHLFP+MP+YNA EAT AVK+VLGEYYQFDETPILKA WREFREC+YVE DD
Sbjct: 306 NITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFRECVYVEEDD 365

Query: 274 ------EGSSSKGVFWFRNKL 289
                 EG  SKGVFWFRNKL
Sbjct: 366 NEEGDSEGFRSKGVFWFRNKL 386

[Close]

BLAST of Bhi10G000309 vs. ExPASy TrEMBL
Match: A0A5D3DDB5 (Bifunctional fatty acid conjugase/Delta(12)-oleate desaturase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold859G00200 PE=3 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 3.8e-125
Identity = 220/261 (84.29%), Postives = 234/261 (89.66%), Query Frame = 0


Query: 34  LIPYFSFKISHRRHHANNASLDRDEVFVPKPKSKISYYFHHLTNPPARLLIIFMTLTLGW 93
           LIPYFSFKISHRRHHAN ASL+RDEVFVPKPKSKI +YF HLTNPPARL II MTLTLGW
Sbjct: 126 LIPYFSFKISHRRHHANTASLNRDEVFVPKPKSKIPWYFKHLTNPPARLFIIVMTLTLGW 185

Query: 94  PLYLAFNRSGRAYDRFTSHYDPNSPIFSEKERLQVQISIAGVVTILYLLYKLAIVKGITW 153
           P+Y+AFN SGR YDRFTSHYDPNSPIF+EKERLQVQIS AGV+TILYLLYK A+ KGI W
Sbjct: 186 PMYIAFNNSGRVYDRFTSHYDPNSPIFTEKERLQVQISNAGVLTILYLLYKFAVTKGIMW 245

Query: 154 VVRMYLLPLTVMNAFVVLILSLQHTHLSLPYYDSSEWDWLRGNLVTVDRDYGMILNKMLH 213
           V+R+YLLPLTVMN FVVLI SLQHTH SLPYYDSS+WDWLRGNLVTVDRDYG ILNKMLH
Sbjct: 246 VIRIYLLPLTVMNVFVVLISSLQHTHPSLPYYDSSQWDWLRGNLVTVDRDYGKILNKMLH 305

Query: 214 NITDTHVIHHLFPTMPYYNAVEATMAVKQVLGEYYQFDETPILKATWREFRECIYVEADD 273
           NITDTHVIHHLFP+MP+YNA EAT AVK+VLGEYYQFDETPILKA WREFREC+YVE DD
Sbjct: 306 NITDTHVIHHLFPSMPHYNAAEATRAVKEVLGEYYQFDETPILKAAWREFRECVYVEEDD 365

Query: 274 ------EGSSSKGVFWFRNKL 289
                 EG  SKGVFWFRNKL
Sbjct: 366 NEEGDSEGFRSKGVFWFRNKL 386

[Close]

BLAST of Bhi10G000309 vs. ExPASy TrEMBL
Match: A0A6J1JD33 (bifunctional fatty acid conjugase/Delta(12)-oleate desaturase-like OS=Cucurbita maxima OX=3661 GN=LOC111484610 PE=4 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 3.0e-114
Identity = 208/259 (80.31%), Postives = 223/259 (86.10%), Query Frame = 0


Query: 34  LIPYFSFKISHRRHHANNASLDRDEVFVPKPKSKISYYFHHLTNPPARLLIIFMTLTLGW 93
           LIPYFSFK SHRRHH+N ASL+RDEVFVPKPKS++ +YF HLTNPPARLLI+F+TLTLGW
Sbjct: 125 LIPYFSFKFSHRRHHSNTASLERDEVFVPKPKSRMPWYFKHLTNPPARLLILFLTLTLGW 184

Query: 94  PLYLAFNRSGRAYDRFTSHYDPNSPIFSEKERLQVQISIAGVVTILYLLYKLAIVKGITW 153
           P+YLAFN SGR YD FTSHY PNSPIFS KE LQV IS AGVV   YLLYKLA  +GI W
Sbjct: 185 PMYLAFNSSGRFYDSFTSHYYPNSPIFSGKEWLQVHISSAGVVAFWYLLYKLAAARGIAW 244

Query: 154 VVRMYLLPLTVMNAFVVLILSLQHTHLSLPYYDSSEWDWLRGNLVTVDRDYGMILNKMLH 213
           V+R+YLLPLTVM AFVVLI SLQHTH SLPYYDSSEWDWLRGNLVTVDRDYG ILNK+ H
Sbjct: 245 VIRIYLLPLTVMYAFVVLISSLQHTHPSLPYYDSSEWDWLRGNLVTVDRDYG-ILNKVFH 304

Query: 214 NITDTHVIHHLFPTMPYYNAVEATMAVKQVLGEYYQFDETPILKATWREFRECIYVEAD- 273
           NITDTHVIHHLFP+MP+YNA EAT AVK+VLGEYY FD TPI KA WREFRECIYVE D 
Sbjct: 305 NITDTHVIHHLFPSMPHYNATEATRAVKEVLGEYYHFDGTPIFKAAWREFRECIYVEPDE 364

Query: 274 DEGSS---SKGVFWFRNKL 289
           DEG S   SKGVFWFRN L
Sbjct: 365 DEGGSASCSKGVFWFRNNL 382

[Close]

BLAST of Bhi10G000309 vs. ExPASy TrEMBL
Match: A0A6J1FY47 (bifunctional fatty acid conjugase/Delta(12)-oleate desaturase-like OS=Cucurbita moschata OX=3662 GN=LOC111448352 PE=4 SV=1)

HSP 1 Score: 417.5 bits (1072), Expect = 4.4e-113
Identity = 209/259 (80.69%), Postives = 224/259 (86.49%), Query Frame = 0


Query: 34  LIPYFSFKISHRRHHANNASLDRDEVFVPKPKSKISYYFHHLTNPPARLLIIFMTLTLGW 93
           LIPYFSFK SHRRHH+N ASL+RDEVFVPKPKS++ +YF HLTNPPARLLIIF+TLTLG 
Sbjct: 125 LIPYFSFKFSHRRHHSNTASLERDEVFVPKPKSRMPWYFKHLTNPPARLLIIFLTLTLGR 184

Query: 94  PLYLAFNRSGRAYDRFTSHYDPNSPIFSEKERLQVQISIAGVVTILYLLYKLAIVKGITW 153
           P+YLAFN SGR YDRFTSHY PNSPIFS KE LQV IS AGVV   YLLYKLA  +GI W
Sbjct: 185 PMYLAFNSSGRFYDRFTSHYYPNSPIFSGKEWLQVHISSAGVVAFWYLLYKLAAARGIAW 244

Query: 154 VVRMYLLPLTVMNAFVVLILSLQHTHLSLPYYDSSEWDWLRGNLVTVDRDYGMILNKMLH 213
           V+R+YLLPLTVM AFVVLI SLQHTH SLPYYDSSEWDWLRGNLVTVDRD G ILNK+ H
Sbjct: 245 VIRIYLLPLTVMYAFVVLISSLQHTHPSLPYYDSSEWDWLRGNLVTVDRDNG-ILNKVFH 304

Query: 214 NITDTHVIHHLFPTMPYYNAVEATMAVKQVLGEYYQFDETPILKATWREFRECIYVEAD- 273
           NITDTHVIHHLFP+MP+YNA+EAT AVK+VLGEYY FD TPILKA WREFRECIYVE D 
Sbjct: 305 NITDTHVIHHLFPSMPHYNAMEATRAVKEVLGEYYHFDGTPILKAAWREFRECIYVEPDE 364

Query: 274 DEGSS---SKGVFWFRNKL 289
           DEG S   SKGVFWFRN L
Sbjct: 365 DEGGSATCSKGVFWFRNNL 382

The following BLAST results are available for this feature:

BLAST of Bhi10G000309 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G12120.1	1.2e-89	60.39	fatty acid desaturase 2	[more]
AT3G12120.2	1.2e-89	60.39	fatty acid desaturase 2	[more]
AT2G29980.1	9.3e-31	36.77	fatty acid desaturase 3	[more]
AT5G05580.1	1.2e-30	38.07	fatty acid desaturase 8	[more]
AT3G11170.1	1.6e-30	37.44	fatty acid desaturase 7	[more]

BLAST of Bhi10G000309 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84UC0	2.0e-118	79.46	Bifunctional fatty acid conjugase/Delta(12)-oleate desaturase OS=Trichosanthes k...	[more]
Q9SP61	2.2e-109	70.27	Delta(12) acyl-lipid conjugase (11E,13E-forming) OS=Momordica charantia OX=3673 ...	[more]
P48631	1.5e-97	66.27	Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 2 OS=Glycine max OX...	[more]
Q84UB9	1.9e-97	63.53	Delta(12)-oleate desaturase OS=Trichosanthes kirilowii OX=3677 GN=FAD2 PE=2 SV=1	[more]
Q8GZC3	2.1e-96	64.96	Delta(12)-fatty-acid desaturase FAD2 OS=Vernicia fordii OX=73154 GN=FAD2 PE=1 SV...	[more]

BLAST of Bhi10G000309 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038902275.1	1.5e-144	100.00	LOW QUALITY PROTEIN: bifunctional fatty acid conjugase/Delta(12)-oleate desatura...	[more]
XP_008449949.1	3.6e-125	85.06	PREDICTED: bifunctional fatty acid conjugase/Delta(12)-oleate desaturase-like [C...	[more]
TYK21657.1	7.9e-125	84.29	bifunctional fatty acid conjugase/Delta(12)-oleate desaturase-like [Cucumis melo...	[more]
XP_011653570.2	7.9e-125	85.06	bifunctional fatty acid conjugase/Delta(12)-oleate desaturase [Cucumis sativus]	[more]
Q84UC0.1	1.5e-115	79.46	RecName: Full=Bifunctional fatty acid conjugase/Delta(12)-oleate desaturase; Sho...	[more]

BLAST of Bhi10G000309 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TJ17	1.7e-125	85.06	Bifunctional fatty acid conjugase/Delta(12)-oleate desaturase-like OS=Cucumis me...	[more]
A0A1S3BMK7	1.7e-125	85.06	bifunctional fatty acid conjugase/Delta(12)-oleate desaturase-like OS=Cucumis me...	[more]
A0A5D3DDB5	3.8e-125	84.29	Bifunctional fatty acid conjugase/Delta(12)-oleate desaturase-like OS=Cucumis me...	[more]
A0A6J1JD33	3.0e-114	80.31	bifunctional fatty acid conjugase/Delta(12)-oleate desaturase-like OS=Cucurbita ...	[more]
A0A6J1FY47	4.4e-113	80.69	bifunctional fatty acid conjugase/Delta(12)-oleate desaturase-like OS=Cucurbita ...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005804	Fatty acid desaturase domain	PFAM	PF00487	FA_desaturase	coord: 32..249 e-value: 1.2E-23 score: 84.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..30
None	No IPR available	PANTHER	PTHR32100	OMEGA-6 FATTY ACID DESATURASE, CHLOROPLASTIC	coord: 33..288
None	No IPR available	PANTHER	PTHR32100:SF40	OLEATE HYDROXYLASE FAH12	coord: 33..288

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi10M000309	Bhi10M000309	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006636	unsaturated fatty acid biosynthetic process
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016717	oxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water