Bhi10G000238 (gene) Wax gourd (B227) v1

Overview
NameBhi10G000238
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptiontRNA-5-taurinomethyluridine 2-sulfurtransferase
Locationchr10: 6752014 .. 6756977 (+)
RNA-Seq ExpressionBhi10G000238
SyntenyBhi10G000238
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTAGAAATCATTTTTCATTTGGCCTTCCAATTGAGTCCATCCGGTACAAAAAAAACCCAATATGTTTTTCGTTGGTTCGTCTTCGGTTGGGAGGTAGAAGAATGCGACGGCGGTGGATAGAAAGGAAGGGGAGATTCAATTACAGTGATGCTGAGACTGCGAGTGAAGCCCGTCGTTTGGTGTTCTCTTCCCATCTCCCATTTCACTTCTCTTCCCCTTTCCCCCTCAATTCTCAGACCTGTCCTCCGCCGGCGCAACCCTCGGCTCTTCCTCACCCGGTTCTCCGCCTCCTCTGCCCTGTGTAAAACCTTGGAATCTTCTTCACCTAAAGGCCTCTCCGATGCCGACTTGGTGCCTTTCCTCTCATGTTCGATGCCTTCCTATCCCCTCAAAGTTGCCGTTCTTCTCAGCGGCGGCGTCGATAGCAGCGTCGCCCTCCGCCTCCTCCACGCTGCTGGCCACTCCTGCACTGCCTTCTACCTCAAAATCTGGTTCCAGGTGCTTCCTCTTTGTTCTTTTAAATGGAATAGGTTATATGGTTGTTGTGTTTTTTTGCAAATTAAGGTAGATGATATTGAGAATGCTGGCGCAATCGTGAGTAATCAAATGGTCATTTTAGCACGAAGTCTTGTAGCAAAAATAATTGGTGTTTAGGATTATAATCAAGAACGCGTGTGGAAGTGTTTCTCTTTTTGTATGTATCAGCTGTGTTGGCAGAGGATAATAGCCTGTTCGTATGACCGCACTTTGATGGGTTTTGTTTTCGTAGGAAGATTTCGAGAATTTTTGGTCAGAATGCCCATGGGACGATGATTTGAAGTATGCTAAAGCTGTTTGTGATCAGGTTTGTTACTATTTTAGCTTATAAATCCATCTGGACTTAAAAAGTGTAATTTGGCCAGCGAAGATCTTAATGAAATAAAGGTTTTCTTGGGAAATTGATCATCGTTCAAGATGTCCATAAGTTATTTCACCATATGGCGCCGTAATTATAGGTTCTGCATAATGGGAAAATATGTGTCTTATTTTTCTGCCGTAGTTTGTGCTCAAGAGAGATCTTAGAATTGAAAAATGTAAATTGGTTTTCAGGTTGATGTACCATTAGAAGTCGTTCATTTGACCGAGGAATATTGGAGGAATGTGGTAAGTGCCTTTTTACAATTACAATTGTGTGCTATAACTTTGATGGAAATCTTAATGATATTAGTACATGACATCCAATCAATTTGGTATACTTGTGCCGGCTGACCCTCTTGCTCATTCGTTCTTTTCTAATAGGATAGGACAAAGTAGTTTTAGGTTCTCTTTTCTGTCTACTGAATATTGCTTAGTTCATGCTTTTAGGTTTCTTATATAATTGAAGAATATCGATGTGGCCGTACTCCAAACCCTGACGTCCTTTGTAACACAAGAATAAAGTTCGGTATGTTCATTGGATGCGCTAGAGATTTTGGCTATATCATCATGTTTTATTTTATTTTATTATTTTGTTATTTGTATGTTTATGATTTCTTTTGTATTGATATAATTTATGGCATCCCCTGCTTTTTCCAGGTGCATTCATGGATGCAATAAGCAATATGGAGTTCGATTATGTTGCTTCAGGGCATTATGCAAATGTTATTCATCCATCTGCTGATCAAATGGATAAACCTTCTGTTTTAGAACTATCCAAGGACATGGTATCTGGAAACAATTGCATGGGCCTTTCTTTCAATCTAACCCATCCAATAGGGAGTATCAGTTATAACTTGCTGAACTTTATTATTTTGTAGTTGATTGTTTCTATAACCCTTTAGGACACTATTTTGCTATTTTTATGACTCTTGTCTATATGCTGTGCATGACAGTTAGTGATGTCTTTAATAATTCAACTTGAAGTCTTGTGTATAAACTCAGTGGAACGATATGACCTATATTGATGTCTTTAATCTATATTTTACGCATGACAGTTAGTGATGTCTTTAGTAATTCAACATGAAGTCTCATGTATAAACTCAGTGGAACAGTATGACCTATATTGAACAACATGGGCCAGTATTTGTTGTAATCCATCTAGAAACAATATCATTAATAAGTATTATGTATTGCTTACCTAGTTCAAGTTTTATTGTCAAACGCATTTATCCTTTCCTTGTTTTTCATTTGTTCCTAACTAAAGATTTCCTATTTTACTTCACTATTATTGTTTTTTAGGTCAAGGATCAAACGTATTTCCTATCGCATCTCTCACAGAATCAGCTTAAACGGCTTTTATTCCCTCTTGGTTGTATTCCAAAGGTTTGTTCAAGCTTACTTTACAGCATTTATTATCATGTAATTTTTAAGATATATCTTATGTTTCAGACTTCAGATTGTAATCCGAGAAGTTCAGCTTCGTAAATCTATATGATTTGGTACTGGCCTTCTAAGCTTCTACAGACATAAACACCTAGCAGCTAACATCGGTTGCATTCCATTGAGGAATTTATGTATTTAATAAAAGTTAAAAATCATATTGGACTTTTCCAATTGATGTAGATATCATATTAGAGCTTTTGGATGTGTTATCTTGCAGCCTGAAGCTATTAAGTTTTTAACTGTTCTCGTATGCAAAACTTATATATAAGTGTTTCAATTTGTAAGTTCATATCATGAGTTAACACTTGCTCCCATCTTAACCAGCTACATTCTTTCAGCTAATGCACTATCTTTTTTGAAGGAAAAAAGAATTACCTGTGTGCTTGTTATCTTGTTAATATGTTTTTCTAGCGTTGTGACTTCAGTAACATGTTTCTTCTCTCAGCCTTATATTCCCTCAACAATCATCTGCAGGATGAGGTTCGCAAACTTGCTGCAAAATTCAACCTGCCAAACAAAGACAGAAAGGATTCACAAGGAATATGCTTTCTTGGGAAGGTACTTTTGGATGTGGTGATTGAAATATCTGTTTGGAGAACTTGTGATTTTCTCAAAAACCATTCCTCAATGCCAGTATTTCTAACTATTTGGTTGCATCGTAAGAATACTCTATTACTTGTTATAACTTTTGCGTATAAAATTTGCTAACTCTTGTTGAAATTTTCTGATGGCTGAACTTTCCCTGAGCTAGTTTTTTATTTCCTTATTCTTCCTAACTGCAAAAATTTTCATTCATCTAGTTTATGAAGATGGGTCTCGTATAAGGTATCTAGTGGGTATAATTCATGTAGAACCTGTTTCTTTGATGTGTAGATAAAGTTTAGTGAATTTGTTGCAAGACATATTGGGGAGGACGAAGGTGTGATACTGGAGGCAGAGTCGGGAGATTTTCTTGGCAAACATCGAGGATTTTGGTTCTACACGATTGGCCAACGTCAAGGTCTAAGACTTCCTGGAGGACCTTGGTATGCCTTTTCAATCAATTCAATGTTCTCTGCTGTTTAGCGGTTGTATCATGTATGATTAATGAAACTTCAGGAACATCTTAAATTCTATTTGGTAGAGTAGATTTGAACGTTACAGTGTGCCAGTTTAGTTTTTAGTGACAAATTACGTTGAATTATTTCTAATTTGGTGCCTCATGAGTTCACATTCTTTACTTGTGTCTCTCGCGGAGTTATATTACAATGACGATTTCAGCTTTTACGCCTTGGTCTAATGCAGGTATGTTGTGGAAAAAGATATAAAGAACAATGTAGTATTTGTGTCGAGAAATTATTTCTCATTTGACAAAAGAAGGCGGGTGTTTCGTGTTGGATCACTAAAATGGCTTACTGGTATGCCCCCAGACCAATTTTCTGGGCTTCAATGTAAGGTAAAGTTTCAGCTTCCATCTCCCATATTTCTCGTTAGTTTCACTACTTCAAATTGTTTGGCTGTAATGGTATAACAGAAAAGGCAATAGTCTTAACATTTTTATTCAAACCTCTCCTGAGAAGCCTTCAGTTTATTCCTGCTAGTTTGTGTCAACAATCATGCTTGGGAAAATTGATAATCCGGAAACGTTGATTAATTACATAATAATTTCGATCTTCCATGATTATTCAATAGAACAAGCATCTCGACGTCATGGGTTTTTCTTTCAAAGAAAGGATTTAAGTTTGGAAGTCAATTTTATTTGATAAGGGATTGTGTAGGGGCTTTCTTTTTTGAGATGGAAAAGTATTAGATTCTATTTTATTTAGGAATTAAACGTATTAGATTGAGTAGATGATCTAGAAATGGTCTGGGGATGCTATGCCTGCTATATTCCACTCATTTGCTTTTAGATTGTGTTATTGGTCTAGATCCTTATCATGTCATTTCCAGTTCCCCATTGTTTCATGATTATATACAATTATTAGGTCCGACATGGCCCGAGTTTCTATAGCTGCAATTTGACAATGGAACACAATGAGGATGGTCGTGAAGACATTGCTGTTGTTCATCTAAGCGAAGACGACCAAGGCCTCGCAGCAGGGCAGTTTGCAGCCTTTTACAAGGGAAGGAGATGCATTGGGTCAGGCGTGATTTTAGAATCATGGGATGATCAGGGGTTTCCTGTATGTCCAAAGGCTGTTGAAAATGCACAAATGGAAGATAAATCGAAGCTTGGTAAACCAATAAAGATAAAAGTGAAACCAGAGAGTGTTCTGAAAGAATCTGAAGCTAAAGATGACACGAAAATACACGGCGAGTCAAATAACCAAGAAACCACTGTGATCAAGCAAATAGGAGAAGTATCTTCAGATGAAGCAATCACCACAGCCCCAATGAAATGGCTTGGAAGCCTTCGGAAGAAGTTGCTGCAGGTATTTTAATTTTGCCCTAGTTTAGAAAACGAATCCTTCATTACAGCCAATGAAAGTGAAACCCACAAGATTACTTTTTATTTATTATTATTATTATGAATCTTTTAACTTGTTTATGACATAACTAAATGTTTGTAAGTTATAAGAACTGATCTTGAAACGTCTGGCCAAATTGAAATCAAACACAAATCCAAATGACAATTTAGTAGGGTA

mRNA sequence

GTAGAAATCATTTTTCATTTGGCCTTCCAATTGAGTCCATCCGGTACAAAAAAAACCCAATATGTTTTTCGTTGGTTCGTCTTCGGTTGGGAGGTAGAAGAATGCGACGGCGGTGGATAGAAAGGAAGGGGAGATTCAATTACAGTGATGCTGAGACTGCGAGTGAAGCCCGTCGTTTGGTGTTCTCTTCCCATCTCCCATTTCACTTCTCTTCCCCTTTCCCCCTCAATTCTCAGACCTGTCCTCCGCCGGCGCAACCCTCGGCTCTTCCTCACCCGGTTCTCCGCCTCCTCTGCCCTGTGTAAAACCTTGGAATCTTCTTCACCTAAAGGCCTCTCCGATGCCGACTTGGTGCCTTTCCTCTCATGTTCGATGCCTTCCTATCCCCTCAAAGTTGCCGTTCTTCTCAGCGGCGGCGTCGATAGCAGCGTCGCCCTCCGCCTCCTCCACGCTGCTGGCCACTCCTGCACTGCCTTCTACCTCAAAATCTGGTTCCAGGAAGATTTCGAGAATTTTTGGTCAGAATGCCCATGGGACGATGATTTGAAGTATGCTAAAGCTGTTTGTGATCAGGTTGATGTACCATTAGAAGTCGTTCATTTGACCGAGGAATATTGGAGGAATGTGGTTTCTTATATAATTGAAGAATATCGATGTGGCCGTACTCCAAACCCTGACGTCCTTTGTAACACAAGAATAAAGTTCGGTGCATTCATGGATGCAATAAGCAATATGGAGTTCGATTATGTTGCTTCAGGGCATTATGCAAATGTTATTCATCCATCTGCTGATCAAATGGATAAACCTTCTGTTTTAGAACTATCCAAGGACATGGTCAAGGATCAAACGTATTTCCTATCGCATCTCTCACAGAATCAGCTTAAACGGCTTTTATTCCCTCTTGGTTGTATTCCAAAGGATGAGGTTCGCAAACTTGCTGCAAAATTCAACCTGCCAAACAAAGACAGAAAGGATTCACAAGGAATATGCTTTCTTGGGAAGATAAAGTTTAGTGAATTTGTTGCAAGACATATTGGGGAGGACGAAGGTGTGATACTGGAGGCAGAGTCGGGAGATTTTCTTGGCAAACATCGAGGATTTTGGTTCTACACGATTGGCCAACGTCAAGGTCTAAGACTTCCTGGAGGACCTTGGTATGTTGTGGAAAAAGATATAAAGAACAATGTAGTATTTGTGTCGAGAAATTATTTCTCATTTGACAAAAGAAGGCGGGTGTTTCGTGTTGGATCACTAAAATGGCTTACTGGTATGCCCCCAGACCAATTTTCTGGGCTTCAATGTAAGGTCCGACATGGCCCGAGTTTCTATAGCTGCAATTTGACAATGGAACACAATGAGGATGGTCGTGAAGACATTGCTGTTGTTCATCTAAGCGAAGACGACCAAGGCCTCGCAGCAGGGCAGTTTGCAGCCTTTTACAAGGGAAGGAGATGCATTGGGTCAGGCGTGATTTTAGAATCATGGGATGATCAGGGGTTTCCTGTATGTCCAAAGGCTGTTGAAAATGCACAAATGGAAGATAAATCGAAGCTTGGTAAACCAATAAAGATAAAAGTGAAACCAGAGAGTGTTCTGAAAGAATCTGAAGCTAAAGATGACACGAAAATACACGGCGAGTCAAATAACCAAGAAACCACTGTGATCAAGCAAATAGGAGAAGTATCTTCAGATGAAGCAATCACCACAGCCCCAATGAAATGGCTTGGAAGCCTTCGGAAGAAGTTGCTGCAGGTATTTTAATTTTGCCCTAGTTTAGAAAACGAATCCTTCATTACAGCCAATGAAAGTGAAACCCACAAGATTACTTTTTATTTATTATTATTATTATGAATCTTTTAACTTGTTTATGACATAACTAAATGTTTGTAAGTTATAAGAACTGATCTTGAAACGTCTGGCCAAATTGAAATCAAACACAAATCCAAATGACAATTTAGTAGGGTA

Coding sequence (CDS)

ATGCTGAGACTGCGAGTGAAGCCCGTCGTTTGGTGTTCTCTTCCCATCTCCCATTTCACTTCTCTTCCCCTTTCCCCCTCAATTCTCAGACCTGTCCTCCGCCGGCGCAACCCTCGGCTCTTCCTCACCCGGTTCTCCGCCTCCTCTGCCCTGTGTAAAACCTTGGAATCTTCTTCACCTAAAGGCCTCTCCGATGCCGACTTGGTGCCTTTCCTCTCATGTTCGATGCCTTCCTATCCCCTCAAAGTTGCCGTTCTTCTCAGCGGCGGCGTCGATAGCAGCGTCGCCCTCCGCCTCCTCCACGCTGCTGGCCACTCCTGCACTGCCTTCTACCTCAAAATCTGGTTCCAGGAAGATTTCGAGAATTTTTGGTCAGAATGCCCATGGGACGATGATTTGAAGTATGCTAAAGCTGTTTGTGATCAGGTTGATGTACCATTAGAAGTCGTTCATTTGACCGAGGAATATTGGAGGAATGTGGTTTCTTATATAATTGAAGAATATCGATGTGGCCGTACTCCAAACCCTGACGTCCTTTGTAACACAAGAATAAAGTTCGGTGCATTCATGGATGCAATAAGCAATATGGAGTTCGATTATGTTGCTTCAGGGCATTATGCAAATGTTATTCATCCATCTGCTGATCAAATGGATAAACCTTCTGTTTTAGAACTATCCAAGGACATGGTCAAGGATCAAACGTATTTCCTATCGCATCTCTCACAGAATCAGCTTAAACGGCTTTTATTCCCTCTTGGTTGTATTCCAAAGGATGAGGTTCGCAAACTTGCTGCAAAATTCAACCTGCCAAACAAAGACAGAAAGGATTCACAAGGAATATGCTTTCTTGGGAAGATAAAGTTTAGTGAATTTGTTGCAAGACATATTGGGGAGGACGAAGGTGTGATACTGGAGGCAGAGTCGGGAGATTTTCTTGGCAAACATCGAGGATTTTGGTTCTACACGATTGGCCAACGTCAAGGTCTAAGACTTCCTGGAGGACCTTGGTATGTTGTGGAAAAAGATATAAAGAACAATGTAGTATTTGTGTCGAGAAATTATTTCTCATTTGACAAAAGAAGGCGGGTGTTTCGTGTTGGATCACTAAAATGGCTTACTGGTATGCCCCCAGACCAATTTTCTGGGCTTCAATGTAAGGTCCGACATGGCCCGAGTTTCTATAGCTGCAATTTGACAATGGAACACAATGAGGATGGTCGTGAAGACATTGCTGTTGTTCATCTAAGCGAAGACGACCAAGGCCTCGCAGCAGGGCAGTTTGCAGCCTTTTACAAGGGAAGGAGATGCATTGGGTCAGGCGTGATTTTAGAATCATGGGATGATCAGGGGTTTCCTGTATGTCCAAAGGCTGTTGAAAATGCACAAATGGAAGATAAATCGAAGCTTGGTAAACCAATAAAGATAAAAGTGAAACCAGAGAGTGTTCTGAAAGAATCTGAAGCTAAAGATGACACGAAAATACACGGCGAGTCAAATAACCAAGAAACCACTGTGATCAAGCAAATAGGAGAAGTATCTTCAGATGAAGCAATCACCACAGCCCCAATGAAATGGCTTGGAAGCCTTCGGAAGAAGTTGCTGCAGGTATTTTAA

Protein sequence

MLRLRVKPVVWCSLPISHFTSLPLSPSILRPVLRRRNPRLFLTRFSASSALCKTLESSSPKGLSDADLVPFLSCSMPSYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLTGMPPDQFSGLQCKVRHGPSFYSCNLTMEHNEDGREDIAVVHLSEDDQGLAAGQFAAFYKGRRCIGSGVILESWDDQGFPVCPKAVENAQMEDKSKLGKPIKIKVKPESVLKESEAKDDTKIHGESNNQETTVIKQIGEVSSDEAITTAPMKWLGSLRKKLLQVF
Homology
[Close]
BLAST of Bhi10G000238 vs. TAIR 10
Match: AT1G51310.1 (transferases;tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferases )

HSP 1 Score: 708.4 bits (1827), Expect = 4.5e-204
Identity = 333/409 (81.42%), Postives = 363/409 (88.75%), Query Frame = 0 

Query: 71  FLSCSMPSYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWSECPWD 130
           +LSCSMP  PL+VAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQE FENFW++CPW+
Sbjct: 72  YLSCSMPEKPLRVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEGFENFWNQCPWE 131

Query: 131 DDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFM 190
           DDLKYAK VC+QVDVPLEVVHLT+EYW  VVSYIIEEYRCGRTPNPDVLCNTRIKFGAFM
Sbjct: 132 DDLKYAKHVCEQVDVPLEVVHLTDEYWERVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFM 191

Query: 191 DAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLF 250
           DAIS+ME+DYV SGHYA V+HP ADQ D  SVLELS+DMVKDQTYFLSHLSQ QLKRLLF
Sbjct: 192 DAISDMEYDYVGSGHYAKVVHPPADQNDASSVLELSQDMVKDQTYFLSHLSQTQLKRLLF 251

Query: 251 PLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGD 310
           PLGC+ KDEVRKLA +F+LPNKDRKDSQGICFLGKIKFS+FV RHIGE EG+ILEAESGD
Sbjct: 252 PLGCVKKDEVRKLATQFDLPNKDRKDSQGICFLGKIKFSDFVCRHIGEMEGIILEAESGD 311

Query: 311 FLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLK 370
           FLG HRGFWFYTIGQRQGLRLPGGPWYVVEKD KNNVVFVSRNY+S DKRRR+FRVGSL+
Sbjct: 312 FLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDTKNNVVFVSRNYYSIDKRRRIFRVGSLR 371

Query: 371 WLTGMPPDQFSGLQCKVRHGPSFYSCNLTMEHNEDGREDIAVVHLSEDDQGLAAGQFAAF 430
           W +G P  +   L+CKVRHGP FYSC+  ME    G  D+AVVHL EDDQGLAAGQFAAF
Sbjct: 372 WNSGKPSGKVRELRCKVRHGPGFYSCSFEME----GDGDVAVVHLDEDDQGLAAGQFAAF 431

Query: 431 YKGRRCIGSGVILESWDDQGFPVCPKAVENAQMEDKSKLGKPIKIKVKP 480
           Y+G  CIGSGVILESWDDQ FPVC KA++ A +EDK+KLGKP+KI   P
Sbjct: 432 YEGTTCIGSGVILESWDDQCFPVCAKALQLAALEDKTKLGKPVKIMTMP 476

[Close]
BLAST of Bhi10G000238 vs. ExPASy Swiss-Prot
Match: Q0SMH1 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia afzelii (strain PKo) OX=390236 GN=mnmA PE=3 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 6.9e-101
Identity = 170/365 (46.58%), Postives = 248/365 (67.95%), Query Frame = 0 

Query: 81  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 140
           +K+AVLLSGGVDSSVAL R+++    S   +YLKIW +++  ++   CPW +DL Y +AV
Sbjct: 1   MKIAVLLSGGVDSSVALYRIINKGYTSIKCYYLKIWLEDEL-SYIGSCPWQEDLNYVEAV 60

Query: 141 CDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 200
           C + +VP E+++  +EY+  VVSY I+E + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61  CSKFNVPYEIINFQKEYYNKVVSYTIKELKNGNTPSPDIFCNQRIKFGAFFEKI-NEQYD 120

Query: 201 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 260
            V +GHYA +        DK   L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYAKI-----QTKDKKFFLKQAKDKIKDQSYFLSHLSQKQMSKLYFPLGTLLKSE 180

Query: 261 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 320
           VR++A   NLPNK+RKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKNINLPNKNRKDSQGICFLGKIKYNEFIKYHLGEKKGNIIEKETGKIIGTHNGYW 240

Query: 321 FYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLTGMPPDQ 380
           F+T+GQR+G++L  GPW+V+EKD++ N++++S N     + +R F V  + W+ G P D 
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYSKQAKRKFLVHEIHWINGTPSD- 300

Query: 381 FSGLQCKVRHGPSFYSCNLTMEHNEDGREDIAVVHLSEDDQGLAAGQFAAFYKGRRCIGS 440
           F   + K+RHG   YSC L +       +++  + L++ D G++ GQFA FYK   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLKLI-----AKNLIEISLNKKDYGISPGQFAIFYKNTECLGG 352

Query: 441 GVILE 445
             I +
Sbjct: 361 AKIFK 352

[Close]
BLAST of Bhi10G000238 vs. ExPASy Swiss-Prot
Match: Q660I8 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia bavariensis (strain ATCC BAA-2496 / DSM 23469 / PBi) OX=290434 GN=mnmA PE=3 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 1.2e-100
Identity = 168/365 (46.03%), Postives = 249/365 (68.22%), Query Frame = 0 

Query: 81  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 140
           +K+AVLLSGGVDSS+AL ++++    +   +YLKIW +++  ++   CPW +DL Y +AV
Sbjct: 1   MKIAVLLSGGVDSSIALYKVINKGYTNIKCYYLKIWLEDEL-SYIGNCPWQEDLNYVEAV 60

Query: 141 CDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 200
           C++ ++P E+++  +EY+  VV Y IEE + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61  CNKFNIPYEIINFQKEYYNKVVIYTIEELKNGNTPSPDIFCNQRIKFGAFFEKI-NEQYD 120

Query: 201 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 260
            V +GHYA +        +K   L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYARI-----QIKEKKFFLKQAKDKIKDQSYFLSHLSQEQMSKLYFPLGTLFKSE 180

Query: 261 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 320
           VR++A   NLPNKDRKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKSINLPNKDRKDSQGICFLGKIKYNEFIKYHLGEKKGNIVEKETGKIIGIHNGYW 240

Query: 321 FYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLTGMPPDQ 380
           F+T+GQR+G++L  GPW+V+EKD++ N++++S N     + +R F V  + W+ GMP D 
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYLKQAKRKFLVHEIHWINGMPSD- 300

Query: 381 FSGLQCKVRHGPSFYSCNLTMEHNEDGREDIAVVHLSEDDQGLAAGQFAAFYKGRRCIGS 440
           F   + K+RHG   YSC L +  N     ++  + L++ D G++ GQFA FYK   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLRLITN-----NLIEIFLNKKDHGISPGQFAIFYKNTECLGG 352

Query: 441 GVILE 445
             I +
Sbjct: 361 AKIFK 352

[Close]
BLAST of Bhi10G000238 vs. ExPASy Swiss-Prot
Match: O51625 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680) OX=224326 GN=mnmA PE=3 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 2.0e-100
Identity = 168/365 (46.03%), Postives = 249/365 (68.22%), Query Frame = 0 

Query: 81  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 140
           +K+AVLLSGGVDSSVAL R+++    +   +YLKIW +++  ++   CPW +DL Y +A+
Sbjct: 1   MKIAVLLSGGVDSSVALYRIINKGYSNIKCYYLKIWVEDEL-SYIGNCPWQEDLNYVEAI 60

Query: 141 CDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 200
           C++ +VP E+++  +EY+  VVSY IEE + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61  CNKFNVPYEIINFQKEYYNKVVSYTIEELKNGNTPSPDIFCNQRIKFGAFFEKI-NSQYD 120

Query: 201 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 260
            V +GHYA +        +   +L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYAKI-----QIKESKFLLKQAKDKIKDQSYFLSHLSQKQMSKLYFPLGTLLKSE 180

Query: 261 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 320
           VR++A   NLPNKDRKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKNINLPNKDRKDSQGICFLGKIKYNEFIKYHLGEKKGNIIEKETGKIIGIHNGYW 240

Query: 321 FYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLTGMPPDQ 380
           F+T+GQR+G++L  GPW+V+EKD++ N++++S N     + +R F V  + W+   P + 
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYLKQAKRKFLVHEIHWINDTPTN- 300

Query: 381 FSGLQCKVRHGPSFYSCNLTMEHNEDGREDIAVVHLSEDDQGLAAGQFAAFYKGRRCIGS 440
           F   + K+RHG   YSC L +  N     ++  + L++ DQG++ GQFA FYK   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLKLITN-----NLMEISLNKKDQGISPGQFAIFYKNTECLGG 352

Query: 441 GVILE 445
             I +
Sbjct: 361 AKIFK 352

[Close]
BLAST of Bhi10G000238 vs. ExPASy Swiss-Prot
Match: B7J2N7 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ZS7) OX=445985 GN=mnmA PE=3 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 2.0e-100
Identity = 168/365 (46.03%), Postives = 249/365 (68.22%), Query Frame = 0 

Query: 81  LKVAVLLSGGVDSSVAL-RLLHAAGHSCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 140
           +K+AVLLSGGVDSSVAL R+++    +   +YLKIW +++  ++   CPW +DL Y +A+
Sbjct: 1   MKIAVLLSGGVDSSVALYRIINKGYSNIKCYYLKIWVEDEL-SYIGNCPWQEDLNYVEAI 60

Query: 141 CDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 200
           C++ +VP E+++  +EY+  VVSY IEE + G TP+PD+ CN RIKFGAF + I N ++D
Sbjct: 61  CNKFNVPYEIINFQKEYYNKVVSYTIEELKNGNTPSPDIFCNQRIKFGAFFEKI-NSQYD 120

Query: 201 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 260
            V +GHYA +        +   +L+ +KD +KDQ+YFLSHLSQ Q+ +L FPLG + K E
Sbjct: 121 LVVTGHYAKI-----QIKESKFLLKQAKDKIKDQSYFLSHLSQKQISKLYFPLGTLLKSE 180

Query: 261 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 320
           VR++A   NLPNKDRKDSQGICFLGKIK++EF+  H+GE +G I+E E+G  +G H G+W
Sbjct: 181 VRQIAKNINLPNKDRKDSQGICFLGKIKYNEFIKYHLGEKKGNIIEKETGKIIGIHNGYW 240

Query: 321 FYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLTGMPPDQ 380
           F+T+GQR+G++L  GPW+V+EKD++ N++++S N     + +R F V  + W+   P + 
Sbjct: 241 FFTVGQRRGIKLSNGPWFVIEKDLEKNIIYISHNENYLKQAKRKFLVHEIHWINDTPTN- 300

Query: 381 FSGLQCKVRHGPSFYSCNLTMEHNEDGREDIAVVHLSEDDQGLAAGQFAAFYKGRRCIGS 440
           F   + K+RHG   YSC L +  N     ++  + L++ DQG++ GQFA FYK   C+G 
Sbjct: 301 FENFKIKIRHGEKKYSCKLKLITN-----NLMEISLNKKDQGISPGQFAIFYKNTECLGG 352

Query: 441 GVILE 445
             I +
Sbjct: 361 AKIFK 352

[Close]
BLAST of Bhi10G000238 vs. ExPASy Swiss-Prot
Match: B2S125 (tRNA-specific 2-thiouridylase MnmA OS=Borrelia hermsii (strain HS1 / DAH) OX=314723 GN=mnmA PE=3 SV=2)

HSP 1 Score: 354.4 bits (908), Expect = 2.3e-96
Identity = 168/366 (45.90%), Postives = 243/366 (66.39%), Query Frame = 0 

Query: 81  LKVAVLLSGGVDSSVALRLLHAAGH-SCTAFYLKIWFQEDFENFWSECPWDDDLKYAKAV 140
           +K+AVLLSGGVDSSVAL  +   G+ +   +YLKIW +++  ++  ECPW++D+ Y +AV
Sbjct: 1   MKIAVLLSGGVDSSVALYTMIQKGYKNIKCYYLKIWLEDEL-SYIGECPWEEDINYVEAV 60

Query: 141 CDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLCNTRIKFGAFMDAISNMEFD 200
           C + +VP E++ L +EY++ VV+Y IEE + G TP+PD+ CN RIKFGAF D I N  +D
Sbjct: 61  CTKFNVPYEIISLQDEYYKRVVTYAIEELKIGNTPSPDIFCNQRIKFGAFFDKI-NEHYD 120

Query: 201 YVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHLSQNQLKRLLFPLGCIPKDE 260
            + +GHYA +     +  +   +L+ +KD +KDQ+YFLSHLS+ Q+ +L FPLG + K E
Sbjct: 121 LIVTGHYAKI-----ENKNNHYILKQAKDKIKDQSYFLSHLSREQISKLHFPLGDLLKTE 180

Query: 261 VRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDEGVILEAESGDFLGKHRGFW 320
           +R++A K +LPNK+RKDSQGICFLGKIK+ EF+  H+GE +G I+E E+G  LG H G+W
Sbjct: 181 IRQIAHKIDLPNKNRKDSQGICFLGKIKYDEFIKYHLGELKGNIIEQETGKILGTHNGYW 240

Query: 321 FYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKRRRVFRVGSLKWLTGMPPDQ 380
           F+TIGQR+G++L  GPW+V EKDI+NN++++S +     + +  F V    W+    P  
Sbjct: 241 FFTIGQRKGIKLSHGPWFVTEKDIQNNIIYISNSVNYLAQGKHQFLVHKTNWIN--KPVN 300

Query: 381 FSGLQCKVRHGPSFYSCNLTMEHNEDGREDIAVVHLSEDDQGLAAGQFAAFYKGRRCIGS 440
              L  K+RHG     C + M  N     DI  V L E D G++ GQF  FY+   C+G 
Sbjct: 301 NIDLSAKIRHGEKKIKCKIEMLKN-----DIIKVDLEEKDYGISPGQFCIFYQEDECLGG 352

Query: 441 GVILES 446
             IL++
Sbjct: 361 AQILQT 352

[Close]
BLAST of Bhi10G000238 vs. NCBI nr
Match: XP_038901654.1 (tRNA-specific 2-thiouridylase MnmA [Benincasa hispida])

HSP 1 Score: 1100.9 bits (2846), Expect = 0.0e+00
Identity = 537/537 (100.00%), Postives = 537/537 (100.00%), Query Frame = 0 

Query: 1   MLRLRVKPVVWCSLPISHFTSLPLSPSILRPVLRRRNPRLFLTRFSASSALCKTLESSSP 60
           MLRLRVKPVVWCSLPISHFTSLPLSPSILRPVLRRRNPRLFLTRFSASSALCKTLESSSP
Sbjct: 1   MLRLRVKPVVWCSLPISHFTSLPLSPSILRPVLRRRNPRLFLTRFSASSALCKTLESSSP 60

Query: 61  KGLSDADLVPFLSCSMPSYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 120
           KGLSDADLVPFLSCSMPSYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF
Sbjct: 61  KGLSDADLVPFLSCSMPSYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 120

Query: 121 ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 180
           ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC
Sbjct: 121 ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 180

Query: 181 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHL 240
           NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHL
Sbjct: 181 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHL 240

Query: 241 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 300
           SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE
Sbjct: 241 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 300

Query: 301 GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR 360
           GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR
Sbjct: 301 GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR 360

Query: 361 RRVFRVGSLKWLTGMPPDQFSGLQCKVRHGPSFYSCNLTMEHNEDGREDIAVVHLSEDDQ 420
           RRVFRVGSLKWLTGMPPDQFSGLQCKVRHGPSFYSCNLTMEHNEDGREDIAVVHLSEDDQ
Sbjct: 361 RRVFRVGSLKWLTGMPPDQFSGLQCKVRHGPSFYSCNLTMEHNEDGREDIAVVHLSEDDQ 420

Query: 421 GLAAGQFAAFYKGRRCIGSGVILESWDDQGFPVCPKAVENAQMEDKSKLGKPIKIKVKPE 480
           GLAAGQFAAFYKGRRCIGSGVILESWDDQGFPVCPKAVENAQMEDKSKLGKPIKIKVKPE
Sbjct: 421 GLAAGQFAAFYKGRRCIGSGVILESWDDQGFPVCPKAVENAQMEDKSKLGKPIKIKVKPE 480

Query: 481 SVLKESEAKDDTKIHGESNNQETTVIKQIGEVSSDEAITTAPMKWLGSLRKKLLQVF 538
           SVLKESEAKDDTKIHGESNNQETTVIKQIGEVSSDEAITTAPMKWLGSLRKKLLQVF
Sbjct: 481 SVLKESEAKDDTKIHGESNNQETTVIKQIGEVSSDEAITTAPMKWLGSLRKKLLQVF 537

[Close]
BLAST of Bhi10G000238 vs. NCBI nr
Match: KAA0041410.1 (tRNA-specific 2-thiouridylase MnmA [Cucumis melo var. makuwa])

HSP 1 Score: 1016.1 bits (2626), Expect = 1.1e-292
Identity = 490/538 (91.08%), Postives = 512/538 (95.17%), Query Frame = 0 

Query: 1   MLRLRVKPVVWCSLPISHFTSLPLSPSILRPVLRRRNPRLFLTRFSASSAL-CKTLESSS 60
           MLRLRV P VWCS+PISHFTSLPLSPSILRP+LR  NPRLF TRFSASSA+   T +SS 
Sbjct: 2   MLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSSL 61

Query: 61  PKGLSDADLVPFLSCSMPSYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120
           PK LSDAD +PFLSCSMP+YPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED
Sbjct: 62  PKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 121

Query: 121 FENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180
           FENFWSECPWDDDLKYAKAVCDQ+DVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL
Sbjct: 122 FENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 181

Query: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 240
           CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH
Sbjct: 182 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 241

Query: 241 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300
           LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED
Sbjct: 242 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 301

Query: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 360
           EGVILEAESGDFLGKHRGFWFYT+GQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK
Sbjct: 302 EGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 361

Query: 361 RRRVFRVGSLKWLTGMPPDQFSGLQCKVRHGPSFYSCNLTMEHNEDGREDIAVVHLSEDD 420
           RRRVFRVGSLKWL+GMPPDQF+GLQCKVRHGP FYSCNLTMEHNEDG ED+AVVHLSEDD
Sbjct: 362 RRRVFRVGSLKWLSGMPPDQFTGLQCKVRHGPGFYSCNLTMEHNEDGHEDVAVVHLSEDD 421

Query: 421 QGLAAGQFAAFYKGRRCIGSGVILESWDDQGFPVCPKAVENAQMEDKSKLGKPIKIKVKP 480
           QGLAAGQFAAFYKGR CIGSGVILESWDDQGFPVC KA+ENAQMEDKSKLGKPIKIKVKP
Sbjct: 422 QGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKP 481

Query: 481 ESVLKESEAKDDTKIHGESNNQETTVIKQIGEVSSDEAITTAPMKWLGSLRKKLLQVF 538
           +S + ES+AKDDTKI  ES N+E TV++Q+ +VS DEA+ T PMKWLGSLRKKLLQ+F
Sbjct: 482 DSGMNESKAKDDTKIQDESRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

[Close]
BLAST of Bhi10G000238 vs. NCBI nr
Match: XP_004142116.1 (uncharacterized protein LOC101204779 isoform X1 [Cucumis sativus] >KGN54175.1 hypothetical protein Csa_017959 [Cucumis sativus])

HSP 1 Score: 1016.1 bits (2626), Expect = 1.1e-292
Identity = 491/537 (91.43%), Postives = 512/537 (95.34%), Query Frame = 0 

Query: 1   MLRLRVKPVVWCSLPISHFTSLPLSPSILRPVLRRRNPRLFLTRFSASSALCKTLESSSP 60
           MLRLRV P VWCS+PISHFTSLPL+PSILRP+LRR NPRLFLTRFSASSA+  T  SSS 
Sbjct: 2   MLRLRVNPAVWCSVPISHFTSLPLTPSILRPLLRRHNPRLFLTRFSASSAVHNTSLSSSS 61

Query: 61  KGLSDADLVPFLSCSMPSYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 120
           K LSDAD +PFLSCSMP+YPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF
Sbjct: 62  KPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 121

Query: 121 ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 180
           ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC
Sbjct: 122 ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 181

Query: 181 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHL 240
           NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHL
Sbjct: 182 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHL 241

Query: 241 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 300
           SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE
Sbjct: 242 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 301

Query: 301 GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR 360
           GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR
Sbjct: 302 GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR 361

Query: 361 RRVFRVGSLKWLTGMPPDQFSGLQCKVRHGPSFYSCNLTMEHNEDGREDIAVVHLSEDDQ 420
           RRVFRVGSLKWL+G PPDQF+ LQCKVRHGPSFYSCNLTMEH+EDG ED+AVVHLSEDDQ
Sbjct: 362 RRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSEDDQ 421

Query: 421 GLAAGQFAAFYKGRRCIGSGVILESWDDQGFPVCPKAVENAQMEDKSKLGKPIKIKVKPE 480
           GLAAGQFAAFYKGR CIGSGVILESWDDQGFPVC KA+ENAQMEDKSKLGKPIKIKVKPE
Sbjct: 422 GLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKPE 481

Query: 481 SVLKESEAKDDTKIHGESNNQETTVIKQIGEVSSDEAITTAPMKWLGSLRKKLLQVF 538
           S +KES+AKDDT+IH +  N+E TV++QI EVS DEA+ T  MKWLGSL+KKLLQ+F
Sbjct: 482 SGMKESKAKDDTEIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKKLLQIF 538

[Close]
BLAST of Bhi10G000238 vs. NCBI nr
Match: XP_008449720.1 (PREDICTED: tRNA-specific 2-thiouridylase MnmA [Cucumis melo] >TYJ96142.1 tRNA-specific 2-thiouridylase MnmA [Cucumis melo var. makuwa])

HSP 1 Score: 1011.5 bits (2614), Expect = 2.7e-291
Identity = 487/538 (90.52%), Postives = 512/538 (95.17%), Query Frame = 0 

Query: 1   MLRLRVKPVVWCSLPISHFTSLPLSPSILRPVLRRRNPRLFLTRFSASSAL-CKTLESSS 60
           MLRLRV P VWCS+PISHFTSLPLSPSILRP+LR  NPRLF TRFSASSA+   T +SS 
Sbjct: 2   MLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSSL 61

Query: 61  PKGLSDADLVPFLSCSMPSYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120
           PK LSDAD +PFLSCSMP+YPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED
Sbjct: 62  PKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 121

Query: 121 FENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180
           FENFWSECPWDDDLKYAKAVCDQ+DVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL
Sbjct: 122 FENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 181

Query: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 240
           CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH
Sbjct: 182 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 241

Query: 241 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300
           LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED
Sbjct: 242 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 301

Query: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 360
           EGVILEAESGDFLGKHRGFWFYT+GQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK
Sbjct: 302 EGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 361

Query: 361 RRRVFRVGSLKWLTGMPPDQFSGLQCKVRHGPSFYSCNLTMEHNEDGREDIAVVHLSEDD 420
           RRRVFRVGSLKWL+GMPPD+F+GLQCKVRHGP FYSCNLTMEHN+DG ED+AVVHLSEDD
Sbjct: 362 RRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDD 421

Query: 421 QGLAAGQFAAFYKGRRCIGSGVILESWDDQGFPVCPKAVENAQMEDKSKLGKPIKIKVKP 480
           QGLAAGQFAAFYKGR CIGSGVILESWDDQGFPVC KA+ENAQMEDKSKLGKPIKIKVKP
Sbjct: 422 QGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKP 481

Query: 481 ESVLKESEAKDDTKIHGESNNQETTVIKQIGEVSSDEAITTAPMKWLGSLRKKLLQVF 538
           +S + ES+AKDDTKI  +S N+E TV++Q+ +VS DEA+ T PMKWLGSLRKKLLQ+F
Sbjct: 482 DSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

[Close]
BLAST of Bhi10G000238 vs. NCBI nr
Match: XP_023513286.1 (uncharacterized protein LOC111777800 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1008.4 bits (2606), Expect = 2.2e-290
Identity = 491/537 (91.43%), Postives = 509/537 (94.79%), Query Frame = 0 

Query: 1   MLRLRVKPVVWCSLPISHFTSLPLSPSILRPVLRRRNPRLFLTRFSASSALCKTLESSSP 60
           MLRLRVKP VWC+LPISHFTSLPL PSILRPVLRR  PRLFLTRFSASSA+ K  ES S 
Sbjct: 1   MLRLRVKP-VWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESYSA 60

Query: 61  KGLSDADLVPFLSCSMPSYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 120
           KGLSD DL PFLSCSMP YPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF
Sbjct: 61  KGLSDGDLEPFLSCSMPRYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 120

Query: 121 ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 180
           ENFWSECPWDDDLKYA+AVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC
Sbjct: 121 ENFWSECPWDDDLKYAEAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 180

Query: 181 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHL 240
           NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHL
Sbjct: 181 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHL 240

Query: 241 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 300
           SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE
Sbjct: 241 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 300

Query: 301 GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR 360
           GVILEAESGDFLGKHRGFWFYTIGQRQGL+LPGGPWYVVEKDI+NNVVFVSRNYFS DKR
Sbjct: 301 GVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKR 360

Query: 361 RRVFRVGSLKWLTGMPPDQFSGLQCKVRHGPSFYSCNLTMEHNEDGREDIAVVHLSEDDQ 420
           RR+FRVGSLKWL+G+ PDQF+ LQCKVRHGPSFYSCNLTME NED  ED+AVVHLSEDDQ
Sbjct: 361 RRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVHLSEDDQ 420

Query: 421 GLAAGQFAAFYKGRRCIGSGVILESWDDQGFPVCPKAVENAQMEDKSKLGKPIKIKVKPE 480
           GLAAGQFAAFY+GRRCIGSGVILESWDDQGFPVC KA+E AQMEDKSKLGKPIKIKVKPE
Sbjct: 421 GLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPE 480

Query: 481 SVLKESEAKDDTKIHGESNNQETTVIKQIGEVSSDEAITTAPMKWLGSLRKKLLQVF 538
           +  KE E KDDTKIHG   NQ+TTV++Q GEV+SDEA+TTAPMKWLGSLRKKLLQVF
Sbjct: 481 NATKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536

[Close]
BLAST of Bhi10G000238 vs. ExPASy TrEMBL
Match: A0A5A7TDN6 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold206G00570 PE=3 SV=1)

HSP 1 Score: 1016.1 bits (2626), Expect = 5.2e-293
Identity = 490/538 (91.08%), Postives = 512/538 (95.17%), Query Frame = 0 

Query: 1   MLRLRVKPVVWCSLPISHFTSLPLSPSILRPVLRRRNPRLFLTRFSASSAL-CKTLESSS 60
           MLRLRV P VWCS+PISHFTSLPLSPSILRP+LR  NPRLF TRFSASSA+   T +SS 
Sbjct: 2   MLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSSL 61

Query: 61  PKGLSDADLVPFLSCSMPSYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120
           PK LSDAD +PFLSCSMP+YPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED
Sbjct: 62  PKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 121

Query: 121 FENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180
           FENFWSECPWDDDLKYAKAVCDQ+DVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL
Sbjct: 122 FENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 181

Query: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 240
           CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH
Sbjct: 182 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 241

Query: 241 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300
           LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED
Sbjct: 242 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 301

Query: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 360
           EGVILEAESGDFLGKHRGFWFYT+GQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK
Sbjct: 302 EGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 361

Query: 361 RRRVFRVGSLKWLTGMPPDQFSGLQCKVRHGPSFYSCNLTMEHNEDGREDIAVVHLSEDD 420
           RRRVFRVGSLKWL+GMPPDQF+GLQCKVRHGP FYSCNLTMEHNEDG ED+AVVHLSEDD
Sbjct: 362 RRRVFRVGSLKWLSGMPPDQFTGLQCKVRHGPGFYSCNLTMEHNEDGHEDVAVVHLSEDD 421

Query: 421 QGLAAGQFAAFYKGRRCIGSGVILESWDDQGFPVCPKAVENAQMEDKSKLGKPIKIKVKP 480
           QGLAAGQFAAFYKGR CIGSGVILESWDDQGFPVC KA+ENAQMEDKSKLGKPIKIKVKP
Sbjct: 422 QGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKP 481

Query: 481 ESVLKESEAKDDTKIHGESNNQETTVIKQIGEVSSDEAITTAPMKWLGSLRKKLLQVF 538
           +S + ES+AKDDTKI  ES N+E TV++Q+ +VS DEA+ T PMKWLGSLRKKLLQ+F
Sbjct: 482 DSGMNESKAKDDTKIQDESRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

[Close]
BLAST of Bhi10G000238 vs. ExPASy TrEMBL
Match: A0A0A0KX16 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis sativus OX=3659 GN=Csa_4G291390 PE=3 SV=1)

HSP 1 Score: 1016.1 bits (2626), Expect = 5.2e-293
Identity = 491/537 (91.43%), Postives = 512/537 (95.34%), Query Frame = 0 

Query: 1   MLRLRVKPVVWCSLPISHFTSLPLSPSILRPVLRRRNPRLFLTRFSASSALCKTLESSSP 60
           MLRLRV P VWCS+PISHFTSLPL+PSILRP+LRR NPRLFLTRFSASSA+  T  SSS 
Sbjct: 2   MLRLRVNPAVWCSVPISHFTSLPLTPSILRPLLRRHNPRLFLTRFSASSAVHNTSLSSSS 61

Query: 61  KGLSDADLVPFLSCSMPSYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 120
           K LSDAD +PFLSCSMP+YPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF
Sbjct: 62  KPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 121

Query: 121 ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 180
           ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC
Sbjct: 122 ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 181

Query: 181 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHL 240
           NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHL
Sbjct: 182 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHL 241

Query: 241 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 300
           SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE
Sbjct: 242 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 301

Query: 301 GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR 360
           GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR
Sbjct: 302 GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR 361

Query: 361 RRVFRVGSLKWLTGMPPDQFSGLQCKVRHGPSFYSCNLTMEHNEDGREDIAVVHLSEDDQ 420
           RRVFRVGSLKWL+G PPDQF+ LQCKVRHGPSFYSCNLTMEH+EDG ED+AVVHLSEDDQ
Sbjct: 362 RRVFRVGSLKWLSGFPPDQFTELQCKVRHGPSFYSCNLTMEHSEDGHEDVAVVHLSEDDQ 421

Query: 421 GLAAGQFAAFYKGRRCIGSGVILESWDDQGFPVCPKAVENAQMEDKSKLGKPIKIKVKPE 480
           GLAAGQFAAFYKGR CIGSGVILESWDDQGFPVC KA+ENAQMEDKSKLGKPIKIKVKPE
Sbjct: 422 GLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKPE 481

Query: 481 SVLKESEAKDDTKIHGESNNQETTVIKQIGEVSSDEAITTAPMKWLGSLRKKLLQVF 538
           S +KES+AKDDT+IH +  N+E TV++QI EVS DEA+ T  MKWLGSL+KKLLQ+F
Sbjct: 482 SGMKESKAKDDTEIHDKLRNEEATVVEQIREVSPDEAMATPSMKWLGSLKKKLLQIF 538

[Close]
BLAST of Bhi10G000238 vs. ExPASy TrEMBL
Match: A0A5D3B8G8 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G001100 PE=3 SV=1)

HSP 1 Score: 1011.5 bits (2614), Expect = 1.3e-291
Identity = 487/538 (90.52%), Postives = 512/538 (95.17%), Query Frame = 0 

Query: 1   MLRLRVKPVVWCSLPISHFTSLPLSPSILRPVLRRRNPRLFLTRFSASSAL-CKTLESSS 60
           MLRLRV P VWCS+PISHFTSLPLSPSILRP+LR  NPRLF TRFSASSA+   T +SS 
Sbjct: 2   MLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSSL 61

Query: 61  PKGLSDADLVPFLSCSMPSYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120
           PK LSDAD +PFLSCSMP+YPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED
Sbjct: 62  PKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 121

Query: 121 FENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180
           FENFWSECPWDDDLKYAKAVCDQ+DVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL
Sbjct: 122 FENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 181

Query: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 240
           CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH
Sbjct: 182 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 241

Query: 241 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300
           LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED
Sbjct: 242 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 301

Query: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 360
           EGVILEAESGDFLGKHRGFWFYT+GQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK
Sbjct: 302 EGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 361

Query: 361 RRRVFRVGSLKWLTGMPPDQFSGLQCKVRHGPSFYSCNLTMEHNEDGREDIAVVHLSEDD 420
           RRRVFRVGSLKWL+GMPPD+F+GLQCKVRHGP FYSCNLTMEHN+DG ED+AVVHLSEDD
Sbjct: 362 RRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDD 421

Query: 421 QGLAAGQFAAFYKGRRCIGSGVILESWDDQGFPVCPKAVENAQMEDKSKLGKPIKIKVKP 480
           QGLAAGQFAAFYKGR CIGSGVILESWDDQGFPVC KA+ENAQMEDKSKLGKPIKIKVKP
Sbjct: 422 QGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKP 481

Query: 481 ESVLKESEAKDDTKIHGESNNQETTVIKQIGEVSSDEAITTAPMKWLGSLRKKLLQVF 538
           +S + ES+AKDDTKI  +S N+E TV++Q+ +VS DEA+ T PMKWLGSLRKKLLQ+F
Sbjct: 482 DSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

[Close]
BLAST of Bhi10G000238 vs. ExPASy TrEMBL
Match: A0A1S3BNL8 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo OX=3656 GN=LOC103491516 PE=3 SV=1)

HSP 1 Score: 1011.5 bits (2614), Expect = 1.3e-291
Identity = 487/538 (90.52%), Postives = 512/538 (95.17%), Query Frame = 0 

Query: 1   MLRLRVKPVVWCSLPISHFTSLPLSPSILRPVLRRRNPRLFLTRFSASSAL-CKTLESSS 60
           MLRLRV P VWCS+PISHFTSLPLSPSILRP+LR  NPRLF TRFSASSA+   T +SS 
Sbjct: 2   MLRLRVNPAVWCSVPISHFTSLPLSPSILRPLLRPHNPRLFFTRFSASSAVQHNTSQSSL 61

Query: 61  PKGLSDADLVPFLSCSMPSYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 120
           PK LSDAD +PFLSCSMP+YPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED
Sbjct: 62  PKPLSDADFLPFLSCSMPTYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQED 121

Query: 121 FENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 180
           FENFWSECPWDDDLKYAKAVCDQ+DVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL
Sbjct: 122 FENFWSECPWDDDLKYAKAVCDQIDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVL 181

Query: 181 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 240
           CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH
Sbjct: 182 CNTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSH 241

Query: 241 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 300
           LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED
Sbjct: 242 LSQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGED 301

Query: 301 EGVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 360
           EGVILEAESGDFLGKHRGFWFYT+GQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK
Sbjct: 302 EGVILEAESGDFLGKHRGFWFYTMGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDK 361

Query: 361 RRRVFRVGSLKWLTGMPPDQFSGLQCKVRHGPSFYSCNLTMEHNEDGREDIAVVHLSEDD 420
           RRRVFRVGSLKWL+GMPPD+F+GLQCKVRHGP FYSCNLTMEHN+DG ED+AVVHLSEDD
Sbjct: 362 RRRVFRVGSLKWLSGMPPDRFTGLQCKVRHGPGFYSCNLTMEHNKDGHEDVAVVHLSEDD 421

Query: 421 QGLAAGQFAAFYKGRRCIGSGVILESWDDQGFPVCPKAVENAQMEDKSKLGKPIKIKVKP 480
           QGLAAGQFAAFYKGR CIGSGVILESWDDQGFPVC KA+ENAQMEDKSKLGKPIKIKVKP
Sbjct: 422 QGLAAGQFAAFYKGRSCIGSGVILESWDDQGFPVCQKAIENAQMEDKSKLGKPIKIKVKP 481

Query: 481 ESVLKESEAKDDTKIHGESNNQETTVIKQIGEVSSDEAITTAPMKWLGSLRKKLLQVF 538
           +S + ES+AKDDTKI  +S N+E TV++Q+ +VS DEA+ T PMKWLGSLRKKLLQ+F
Sbjct: 482 DSGMNESKAKDDTKIQDKSRNEEATVVEQMRKVSPDEAMATTPMKWLGSLRKKLLQIF 539

[Close]
BLAST of Bhi10G000238 vs. ExPASy TrEMBL
Match: A0A6J1FY63 (tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucurbita moschata OX=3662 GN=LOC111448983 PE=3 SV=1)

HSP 1 Score: 1006.9 bits (2602), Expect = 3.2e-290
Identity = 490/537 (91.25%), Postives = 509/537 (94.79%), Query Frame = 0 

Query: 1   MLRLRVKPVVWCSLPISHFTSLPLSPSILRPVLRRRNPRLFLTRFSASSALCKTLESSSP 60
           MLRLRV P VWC+LPISHFTSLPL PSILRPVLRR  PRLFLTRFSASSA+ K  ESSS 
Sbjct: 1   MLRLRVNP-VWCTLPISHFTSLPLRPSILRPVLRRHRPRLFLTRFSASSAVHKASESSSA 60

Query: 61  KGLSDADLVPFLSCSMPSYPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 120
           KGLSD DL PFLSCSMP +PLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF
Sbjct: 61  KGLSDGDLEPFLSCSMPRHPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDF 120

Query: 121 ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 180
           ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC
Sbjct: 121 ENFWSECPWDDDLKYAKAVCDQVDVPLEVVHLTEEYWRNVVSYIIEEYRCGRTPNPDVLC 180

Query: 181 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMDKPSVLELSKDMVKDQTYFLSHL 240
           NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQM+KPSVLELSKDMVKDQTYFLSHL
Sbjct: 181 NTRIKFGAFMDAISNMEFDYVASGHYANVIHPSADQMEKPSVLELSKDMVKDQTYFLSHL 240

Query: 241 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 300
           SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE
Sbjct: 241 SQNQLKRLLFPLGCIPKDEVRKLAAKFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEDE 300

Query: 301 GVILEAESGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSFDKR 360
           GVILEAESGDFLGKHRGFWFYTIGQRQGL+LPGGPWYVVEKDI+NNVVFVSRNYFS DKR
Sbjct: 301 GVILEAESGDFLGKHRGFWFYTIGQRQGLKLPGGPWYVVEKDIRNNVVFVSRNYFSLDKR 360

Query: 361 RRVFRVGSLKWLTGMPPDQFSGLQCKVRHGPSFYSCNLTMEHNEDGREDIAVVHLSEDDQ 420
           RR+FRVGSLKWL+G+ PDQF+ LQCKVRHGPSFYSCNLTME NED  ED+AVVHLSEDDQ
Sbjct: 361 RRLFRVGSLKWLSGLVPDQFTELQCKVRHGPSFYSCNLTMERNEDSDEDVAVVHLSEDDQ 420

Query: 421 GLAAGQFAAFYKGRRCIGSGVILESWDDQGFPVCPKAVENAQMEDKSKLGKPIKIKVKPE 480
           GLAAGQFAAFY+GRRCIGSGVILESWDDQGFPVC KA+E AQMEDKSKLGKPIKIKVKPE
Sbjct: 421 GLAAGQFAAFYEGRRCIGSGVILESWDDQGFPVCAKALEIAQMEDKSKLGKPIKIKVKPE 480

Query: 481 SVLKESEAKDDTKIHGESNNQETTVIKQIGEVSSDEAITTAPMKWLGSLRKKLLQVF 538
           +  KE E KDDTKIHG   NQ+TTV++Q GEV+SDEA+TTAPMKWLGSLRKKLLQVF
Sbjct: 481 NATKECEPKDDTKIHGVLKNQQTTVVEQTGEVASDEALTTAPMKWLGSLRKKLLQVF 536

The following BLAST results are available for this feature:
BLAST of Bhi10G000238 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G51310.14.5e-20481.42transferases;tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferases [more]
BLAST of Bhi10G000238 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q0SMH16.9e-10146.58tRNA-specific 2-thiouridylase MnmA OS=Borrelia afzelii (strain PKo) OX=390236 GN... [more]
Q660I81.2e-10046.03tRNA-specific 2-thiouridylase MnmA OS=Borrelia bavariensis (strain ATCC BAA-2496... [more]
O516252.0e-10046.03tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ATCC 35210 / ... [more]
B7J2N72.0e-10046.03tRNA-specific 2-thiouridylase MnmA OS=Borrelia burgdorferi (strain ZS7) OX=44598... [more]
B2S1252.3e-9645.90tRNA-specific 2-thiouridylase MnmA OS=Borrelia hermsii (strain HS1 / DAH) OX=314... [more]
BLAST of Bhi10G000238 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038901654.10.0e+00100.00tRNA-specific 2-thiouridylase MnmA [Benincasa hispida][more]
KAA0041410.11.1e-29291.08tRNA-specific 2-thiouridylase MnmA [Cucumis melo var. makuwa][more]
XP_004142116.11.1e-29291.43uncharacterized protein LOC101204779 isoform X1 [Cucumis sativus] >KGN54175.1 hy... [more]
XP_008449720.12.7e-29190.52PREDICTED: tRNA-specific 2-thiouridylase MnmA [Cucumis melo] >TYJ96142.1 tRNA-sp... [more]
XP_023513286.12.2e-29091.43uncharacterized protein LOC111777800 [Cucurbita pepo subsp. pepo][more]
BLAST of Bhi10G000238 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5A7TDN65.2e-29391.08tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1... [more]
A0A0A0KX165.2e-29391.43tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis sativus OX=3659 GN=Cs... [more]
A0A5D3B8G81.3e-29190.52tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo var. makuwa OX=1... [more]
A0A1S3BNL81.3e-29190.52tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucumis melo OX=3656 GN=LOC10... [more]
A0A6J1FY633.2e-29091.25tRNA-5-taurinomethyluridine 2-sulfurtransferase OS=Cucurbita moschata OX=3662 GN... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023382Adenine nucleotide alpha hydrolase-like domainsGENE3D2.30.30.280coord: 295..354
e-value: 7.0E-19
score: 69.0
IPR004506tRNA-specific 2-thiouridylaseTIGRFAMTIGR00420TIGR00420coord: 82..442
e-value: 1.3E-109
score: 364.3
IPR004506tRNA-specific 2-thiouridylaseHAMAPMF_00144tRNA_thiouridyl_MnmAcoord: 81..443
score: 33.318176
IPR004506tRNA-specific 2-thiouridylaseCDDcd01998tRNA_Me_transcoord: 82..442
e-value: 6.24316E-160
score: 456.984
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 73..294
e-value: 2.2E-85
score: 287.6
NoneNo IPR availableGENE3D2.40.30.10Translation factorscoord: 361..450
e-value: 6.4E-14
score: 53.3
NoneNo IPR availablePFAMPF03054tRNA_Me_transcoord: 82..442
e-value: 2.9E-126
score: 421.0
NoneNo IPR availablePANTHERPTHR43052FAMILY NOT NAMEDcoord: 70..475
NoneNo IPR availableSUPERFAMILY52402Adenine nucleotide alpha hydrolases-likecoord: 82..327

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi10M000238Bhi10M000238mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008033 tRNA processing
molecular_function GO:0005524 ATP binding
molecular_function GO:0061708 tRNA-5-taurinomethyluridine 2-sulfurtransferase
molecular_function GO:0000049 tRNA binding
molecular_function GO:0016783 sulfurtransferase activity
molecular_function GO:0016740 transferase activity