Bhi10G000213 (gene) Wax gourd (B227) v1

Overview
NameBhi10G000213
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionUbiquitin-conjugating enzyme
Locationchr10: 6172971 .. 6175023 (-)
RNA-Seq ExpressionBhi10G000213
SyntenyBhi10G000213
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATTTTTGAATATTTATCCTTCAAATCAAATCCCTAATTTCTTCGTCTTTTTCCTTCGTTTTAAGTCCCCCAATTCCTCTCTCCCCTCACTACCAAAATCTCATTGATTCTTCTTTGTGGGGGAGAATTTCTTGGATCTCCTTCTTCAATTCCTTCATTTCTTCGTTTCTAATTCGGATCACAGAGAGAATGGCGGCGATCAATCGATATACTGACAACACAACCCCGGCTAACCCGGTTGTTTCTCCAGCGAAGCAATCCTTACCGCCGGCCAAAACCGCTGACTCTCAGTCCGTTCTCAAGAGGTATAAACATTTCCGCTTTTTTTCCAATTCGGTTACTATATCACTGTGGATCGATCGCTTTCCTGTTCATCGCATCTGATTTCTACTTTATGTGATTTTGGGGCTTTGTTGAACTTTAAGCTGGATGAGGTAGTTCTAGGGTTCTTGATCTTGTAATCTTTTGAACTGATTTTGATATCGATTGTTCTGTGTGTCTTTCTTACCGATCTTTTTAGGGTTTTAGGGGTAATTTGAGGATCTTTTGAGGTAGTCATGTTCTTTCCCCTTAATTTTGCTCCGTCGCCATATGGGGTCTATGCAATTTAAGAAAATAGTGAAGAACCAATCTTACTGGGAACTCCGTCTGTGTTCAATCAACTAAGAACTAACAATCTTCCTCTTATTTTTGTTGTGTTTTCTTTTTACACAGGCTGCAGTCTGAATTAATGGCCTTAATGGTAAGACTTGCTACCATCTGATGTCTTGGACTGATAATTTTTTAAGTAGTTGGAAATGTGATTATTTAAGACTCCATGTTTTACCATATTGCAGATGAGTGGAGACTCTGGGATCTCAGCCTTTCCTGAGGAAGATAACATTTTCTGCTGGAAAGGGACAATAACTGGAAGCAAAGAAACAGTGTTTGAAGGAACAGAGTACAGGCTATCCTTTTCATTCCCTAACGATTACCCTTTTAAGCCTCCAAAGGTGAAGTTTGAGACCGGCTGCTTTCACCCAAATGTGGACGTTTATGGCAATATTTGCTTGGATATACTTCAGGTAATTCAGCTTTGGTGAAATCTTCAATATTGAAATGATAACTTCTCTGATTTGTTAGGACTAAAGCAACCTAAAGTGAGAATTGTTTCATTTTTTGCAGGATAAATGGTCATCAGCATATGATGTGAGAACCATAACTTCTATCCATCCAAAGTCTGCTTGGAGGTAAGCCCACACCACACCCCCCCCCCCCCCCCCCCCAAAAAAAAAAAAAAAAAAAAAAGTATATATATTTATTTATTATGTATGTGAAGAAACCATACTTCCTTGACTTGTAGATGGTCTTATTTCAACATGAATCCCTTAAATTTGGAATGGAATATAGAATGTTAAATTACAAATTTAGTCCTTATTGTTTGATGTTGCTTCAAAGTTTTATTAAACTAAGGGGACTAAATTCGAACTTTTAAAACCATAGGGACTAAACTCTAACTTTTTCCAAATCATAGGTACTAAATTTGTAATTTAACCAATATAGAATATGTAATGAGACTGCCGTTTATTTGCAGAACCAAACATAAGTTCACCTCTAAACACCCAGGCAGCCCAACTGTGGAGTAATCAAGAAGGTGGGTTTCTATTATTGTTCTTGTCTCATTGAATTGGCTGCCATGTAATGAAAGTTGGCTCACTCATATTTCTGTGTTTGTAGAATATCGGAAAATGGTGGAGAAATTGTACAAGCCTCCAACAGCAGCTTAGCTTTGTTTTTAGACTAGGATTTGTGTCTCTCAACTCTTTTTGCGCTAGAGAGGAGATTCCCAGATGATTTTTTTTCTCTTTCCTTTCAGTGTACGATCTTCACTTCTTTTACTCGCTGGATTTGTTGCAGATATATGTTGTCTACTGTAATATGTGGAAATTTCAAGTCGTTAGCTAATATTAATTTGTTCTCATTTCAAACTTCATCATCTACACTGCTGCCATGCTCGTTTTGAAGTCCTTGAAACCAACATGATGCTAAAAATTCATAATCAGTGTTCTCAAA

mRNA sequence

AAATTTTTGAATATTTATCCTTCAAATCAAATCCCTAATTTCTTCGTCTTTTTCCTTCGTTTTAAGTCCCCCAATTCCTCTCTCCCCTCACTACCAAAATCTCATTGATTCTTCTTTGTGGGGGAGAATTTCTTGGATCTCCTTCTTCAATTCCTTCATTTCTTCGTTTCTAATTCGGATCACAGAGAGAATGGCGGCGATCAATCGATATACTGACAACACAACCCCGGCTAACCCGGTTGTTTCTCCAGCGAAGCAATCCTTACCGCCGGCCAAAACCGCTGACTCTCAGTCCGTTCTCAAGAGGCTGCAGTCTGAATTAATGGCCTTAATGATGAGTGGAGACTCTGGGATCTCAGCCTTTCCTGAGGAAGATAACATTTTCTGCTGGAAAGGGACAATAACTGGAAGCAAAGAAACAGTGTTTGAAGGAACAGAGTACAGGCTATCCTTTTCATTCCCTAACGATTACCCTTTTAAGCCTCCAAAGGTGAAGTTTGAGACCGGCTGCTTTCACCCAAATGTGGACGTTTATGGCAATATTTGCTTGGATATACTTCAGGATAAATGGTCATCAGCATATGATGTGAGAACCATAACTTCTATCCATCCAAAGTCTGCTTGGAGAACCAAACATAAGTTCACCTCTAAACACCCAGGCAGCCCAACTGTGGAGTAATCAAGAAGAATATCGGAAAATGGTGGAGAAATTGTACAAGCCTCCAACAGCAGCTTAGCTTTGTTTTTAGACTAGGATTTGTGTCTCTCAACTCTTTTTGCGCTAGAGAGGAGATTCCCAGATGATTTTTTTTCTCTTTCCTTTCAGTGTACGATCTTCACTTCTTTTACTCGCTGGATTTGTTGCAGATATATGTTGTCTACTGTAATATGTGGAAATTTCAAGTCGTTAGCTAATATTAATTTGTTCTCATTTCAAACTTCATCATCTACACTGCTGCCATGCTCGTTTTGAAGTCCTTGAAACCAACATGATGCTAAAAATTCATAATCAGTGTTCTCAAA

Coding sequence (CDS)

ATGGCGGCGATCAATCGATATACTGACAACACAACCCCGGCTAACCCGGTTGTTTCTCCAGCGAAGCAATCCTTACCGCCGGCCAAAACCGCTGACTCTCAGTCCGTTCTCAAGAGGCTGCAGTCTGAATTAATGGCCTTAATGATGAGTGGAGACTCTGGGATCTCAGCCTTTCCTGAGGAAGATAACATTTTCTGCTGGAAAGGGACAATAACTGGAAGCAAAGAAACAGTGTTTGAAGGAACAGAGTACAGGCTATCCTTTTCATTCCCTAACGATTACCCTTTTAAGCCTCCAAAGGTGAAGTTTGAGACCGGCTGCTTTCACCCAAATGTGGACGTTTATGGCAATATTTGCTTGGATATACTTCAGGATAAATGGTCATCAGCATATGATGTGAGAACCATAACTTCTATCCATCCAAAGTCTGCTTGGAGAACCAAACATAAGTTCACCTCTAAACACCCAGGCAGCCCAACTGTGGAGTAA

Protein sequence

MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPEEDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICLDILQDKWSSAYDVRTITSIHPKSAWRTKHKFTSKHPGSPTVE
Homology
BLAST of Bhi10G000213 vs. TAIR 10
Match: AT1G50490.1 (ubiquitin-conjugating enzyme 20 )

HSP 1 Score: 226.9 bits (577), Expect = 1.2e-59
Identity = 110/136 (80.88%), Postives = 114/136 (83.82%), Query Frame = 0

Query: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAA+N Y  NT    P  + +KQ   P KT DSQSVLKRLQSELM LMM G  GISAFPE
Sbjct: 1   MAAVNGYQGNTPADPPASNGSKQPAAPTKTVDSQSVLKRLQSELMGLMMGGGPGISAFPE 60

Query: 61  EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSK+TVFEGTEYRLS SF NDYPFKPPKVKFET CFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSLSFSNDYPFKPPKVKFETCCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTI 137
           DILQDKWSSAYDVRTI
Sbjct: 121 DILQDKWSSAYDVRTI 136

BLAST of Bhi10G000213 vs. TAIR 10
Match: AT3G20060.1 (ubiquitin-conjugating enzyme19 )

HSP 1 Score: 224.9 bits (572), Expect = 4.5e-59
Identity = 109/137 (79.56%), Postives = 115/137 (83.94%), Query Frame = 0

Query: 1   MAAINRYTDNTTPA-NPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFP 60
           MA +N YT NT  A  P  + +KQS PP KT DS SVLKRLQSELM LMM  D GISAFP
Sbjct: 1   MATVNGYTGNTPAATTPAATGSKQSAPPTKTVDSHSVLKRLQSELMGLMMGADPGISAFP 60

Query: 61  EEDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNIC 120
           EEDNIFCWKGTITGSK+TVFEGTEYRLS +F NDYPFK PKVKFET CFHPNVD+YGNIC
Sbjct: 61  EEDNIFCWKGTITGSKDTVFEGTEYRLSLTFSNDYPFKSPKVKFETCCFHPNVDLYGNIC 120

Query: 121 LDILQDKWSSAYDVRTI 137
           LDILQDKWSSAYDVRTI
Sbjct: 121 LDILQDKWSSAYDVRTI 137

BLAST of Bhi10G000213 vs. TAIR 10
Match: AT3G20060.2 (ubiquitin-conjugating enzyme19 )

HSP 1 Score: 179.9 bits (455), Expect = 1.7e-45
Identity = 81/92 (88.04%), Postives = 84/92 (91.30%), Query Frame = 0

Query: 45  MALMMSGDSGISAFPEEDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFE 104
           M LMM  D GISAFPEEDNIFCWKGTITGSK+TVFEGTEYRLS +F NDYPFK PKVKFE
Sbjct: 1   MGLMMGADPGISAFPEEDNIFCWKGTITGSKDTVFEGTEYRLSLTFSNDYPFKSPKVKFE 60

Query: 105 TGCFHPNVDVYGNICLDILQDKWSSAYDVRTI 137
           T CFHPNVD+YGNICLDILQDKWSSAYDVRTI
Sbjct: 61  TCCFHPNVDLYGNICLDILQDKWSSAYDVRTI 92

BLAST of Bhi10G000213 vs. TAIR 10
Match: AT1G14400.1 (ubiquitin carrier protein 1 )

HSP 1 Score: 99.4 bits (246), Expect = 2.9e-21
Identity = 47/103 (45.63%), Postives = 63/103 (61.17%), Query Frame = 0

Query: 38  KRLQSELMALMMSGDSGISAFPEEDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFK 97
           KRL  +   L     +GIS  P+++NI  W   I G  +T ++G  ++LS  F  DYP K
Sbjct: 7   KRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLSLQFSEDYPNK 66

Query: 98  PPKVKFETGCFHPNVDVYGNICLDILQDKWSSAYDVRTI-TSI 140
           PP V+F +  FHPN+   G+ICLDILQ++WS  YDV  I TSI
Sbjct: 67  PPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSI 109

BLAST of Bhi10G000213 vs. TAIR 10
Match: AT1G14400.2 (ubiquitin carrier protein 1 )

HSP 1 Score: 99.4 bits (246), Expect = 2.9e-21
Identity = 47/103 (45.63%), Postives = 63/103 (61.17%), Query Frame = 0

Query: 38  KRLQSELMALMMSGDSGISAFPEEDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFK 97
           KRL  +   L     +GIS  P+++NI  W   I G  +T ++G  ++LS  F  DYP K
Sbjct: 7   KRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLSLQFSEDYPNK 66

Query: 98  PPKVKFETGCFHPNVDVYGNICLDILQDKWSSAYDVRTI-TSI 140
           PP V+F +  FHPN+   G+ICLDILQ++WS  YDV  I TSI
Sbjct: 67  PPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSI 109

BLAST of Bhi10G000213 vs. ExPASy Swiss-Prot
Match: Q8L7T3 (Ubiquitin-conjugating enzyme E2 20 OS=Arabidopsis thaliana OX=3702 GN=UBC20 PE=2 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 1.7e-58
Identity = 110/136 (80.88%), Postives = 114/136 (83.82%), Query Frame = 0

Query: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAA+N Y  NT    P  + +KQ   P KT DSQSVLKRLQSELM LMM G  GISAFPE
Sbjct: 1   MAAVNGYQGNTPADPPASNGSKQPAAPTKTVDSQSVLKRLQSELMGLMMGGGPGISAFPE 60

Query: 61  EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSK+TVFEGTEYRLS SF NDYPFKPPKVKFET CFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSLSFSNDYPFKPPKVKFETCCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTI 137
           DILQDKWSSAYDVRTI
Sbjct: 121 DILQDKWSSAYDVRTI 136

BLAST of Bhi10G000213 vs. ExPASy Swiss-Prot
Match: Q9LJZ5 (Ubiquitin-conjugating enzyme E2 19 OS=Arabidopsis thaliana OX=3702 GN=UBC19 PE=1 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 6.4e-58
Identity = 109/137 (79.56%), Postives = 115/137 (83.94%), Query Frame = 0

Query: 1   MAAINRYTDNTTPA-NPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFP 60
           MA +N YT NT  A  P  + +KQS PP KT DS SVLKRLQSELM LMM  D GISAFP
Sbjct: 1   MATVNGYTGNTPAATTPAATGSKQSAPPTKTVDSHSVLKRLQSELMGLMMGADPGISAFP 60

Query: 61  EEDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNIC 120
           EEDNIFCWKGTITGSK+TVFEGTEYRLS +F NDYPFK PKVKFET CFHPNVD+YGNIC
Sbjct: 61  EEDNIFCWKGTITGSKDTVFEGTEYRLSLTFSNDYPFKSPKVKFETCCFHPNVDLYGNIC 120

Query: 121 LDILQDKWSSAYDVRTI 137
           LDILQDKWSSAYDVRTI
Sbjct: 121 LDILQDKWSSAYDVRTI 137

BLAST of Bhi10G000213 vs. ExPASy Swiss-Prot
Match: Q4R9D1 (Ubiquitin-conjugating enzyme E2 C OS=Macaca fascicularis OX=9541 GN=UBE2C PE=2 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 2.4e-36
Identity = 75/129 (58.14%), Postives = 89/129 (68.99%), Query Frame = 0

Query: 8   TDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPEEDNIFCW 67
           + N  PA   V+ A++   P+  A    V KRLQ ELM LMMSGD GISAFPE DN+F W
Sbjct: 3   SQNRDPAATSVTAARKGAEPSGGAARGPVGKRLQQELMTLMMSGDKGISAFPESDNLFKW 62

Query: 68  KGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICLDILQDKW 127
            GTI G+  TV+E   Y+LS  FP+ YP+  P VKF T C+HPNVD  GNICLDIL+DKW
Sbjct: 63  VGTIHGAAGTVYEDLRYKLSLEFPSGYPYNAPTVKFLTPCYHPNVDTQGNICLDILKDKW 122

Query: 128 SSAYDVRTI 137
           S+ YDVRTI
Sbjct: 123 SALYDVRTI 131

BLAST of Bhi10G000213 vs. ExPASy Swiss-Prot
Match: Q32PA5 (Ubiquitin-conjugating enzyme E2 C OS=Bos taurus OX=9913 GN=UBE2C PE=2 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 6.9e-36
Identity = 74/129 (57.36%), Postives = 88/129 (68.22%), Query Frame = 0

Query: 8   TDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPEEDNIFCW 67
           + N  P    V+ A++   P+  A    V KRLQ ELM LMMSGD GISAFPE DN+F W
Sbjct: 3   SQNRDPVAASVAAARKGAEPSGGAARGPVGKRLQQELMTLMMSGDKGISAFPESDNLFKW 62

Query: 68  KGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICLDILQDKW 127
            GTI G+  TV+E   Y+LS  FP+ YP+  P VKF T C+HPNVD  GNICLDIL+DKW
Sbjct: 63  VGTIHGAAGTVYEDLRYKLSLEFPSGYPYNAPTVKFLTPCYHPNVDTQGNICLDILKDKW 122

Query: 128 SSAYDVRTI 137
           S+ YDVRTI
Sbjct: 123 SALYDVRTI 131

BLAST of Bhi10G000213 vs. ExPASy Swiss-Prot
Match: O00762 (Ubiquitin-conjugating enzyme E2 C OS=Homo sapiens OX=9606 GN=UBE2C PE=1 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 6.9e-36
Identity = 74/129 (57.36%), Postives = 89/129 (68.99%), Query Frame = 0

Query: 8   TDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPEEDNIFCW 67
           + N  PA   V+ A++   P+  A    V KRLQ ELM LMMSGD GISAFPE DN+F W
Sbjct: 3   SQNRDPAATSVAAARKGAEPSGGAARGPVGKRLQQELMTLMMSGDKGISAFPESDNLFKW 62

Query: 68  KGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICLDILQDKW 127
            GTI G+  TV+E   Y+LS  FP+ YP+  P VKF T C+HPNVD  GNICLDIL++KW
Sbjct: 63  VGTIHGAAGTVYEDLRYKLSLEFPSGYPYNAPTVKFLTPCYHPNVDTQGNICLDILKEKW 122

Query: 128 SSAYDVRTI 137
           S+ YDVRTI
Sbjct: 123 SALYDVRTI 131

BLAST of Bhi10G000213 vs. NCBI nr
Match: XP_038900928.1 (LOW QUALITY PROTEIN: ubiquitin-conjugating enzyme E2 20 [Benincasa hispida])

HSP 1 Score: 283.1 bits (723), Expect = 1.5e-72
Identity = 136/136 (100.00%), Postives = 136/136 (100.00%), Query Frame = 0

Query: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTI 137
           DILQDKWSSAYDVRTI
Sbjct: 121 DILQDKWSSAYDVRTI 136

BLAST of Bhi10G000213 vs. NCBI nr
Match: XP_022957735.1 (ubiquitin-conjugating enzyme E2 20-like [Cucurbita moschata] >KAG6605929.1 Ubiquitin-conjugating enzyme E2 19, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035878.1 Ubiquitin-conjugating enzyme E2 19, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 282.0 bits (720), Expect = 3.4e-72
Identity = 135/136 (99.26%), Postives = 136/136 (100.00%), Query Frame = 0

Query: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSK+TVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTI 137
           DILQDKWSSAYDVRTI
Sbjct: 121 DILQDKWSSAYDVRTI 136

BLAST of Bhi10G000213 vs. NCBI nr
Match: XP_023533942.1 (ubiquitin-conjugating enzyme E2 20-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 281.6 bits (719), Expect = 4.4e-72
Identity = 134/136 (98.53%), Postives = 136/136 (100.00%), Query Frame = 0

Query: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSK+TVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVD+YGNICL
Sbjct: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDIYGNICL 120

Query: 121 DILQDKWSSAYDVRTI 137
           DILQDKWSSAYDVRTI
Sbjct: 121 DILQDKWSSAYDVRTI 136

BLAST of Bhi10G000213 vs. NCBI nr
Match: ADN33927.1 (ubiquitin-conjugating enzyme [Cucumis melo subsp. melo])

HSP 1 Score: 281.2 bits (718), Expect = 5.7e-72
Identity = 135/136 (99.26%), Postives = 135/136 (99.26%), Query Frame = 0

Query: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNT PANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTI 137
           DILQDKWSSAYDVRTI
Sbjct: 121 DILQDKWSSAYDVRTI 136

BLAST of Bhi10G000213 vs. NCBI nr
Match: XP_004140207.1 (ubiquitin-conjugating enzyme E2 20 [Cucumis sativus] >XP_008449688.1 PREDICTED: ubiquitin-conjugating enzyme E2 20 [Cucumis melo] >KAA0061782.1 ubiquitin-conjugating enzyme E2 20-like isoform X3 [Cucumis melo var. makuwa] >KGN48113.1 hypothetical protein Csa_003824 [Cucumis sativus] >TYJ96123.1 ubiquitin-conjugating enzyme E2 20-like isoform X3 [Cucumis melo var. makuwa])

HSP 1 Score: 281.2 bits (718), Expect = 5.7e-72
Identity = 135/136 (99.26%), Postives = 135/136 (99.26%), Query Frame = 0

Query: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNT PANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTI 137
           DILQDKWSSAYDVRTI
Sbjct: 121 DILQDKWSSAYDVRTI 136

BLAST of Bhi10G000213 vs. ExPASy TrEMBL
Match: A0A6J1H1C8 (ubiquitin-conjugating enzyme E2 20-like OS=Cucurbita moschata OX=3662 GN=LOC111459191 PE=3 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 1.6e-72
Identity = 135/136 (99.26%), Postives = 136/136 (100.00%), Query Frame = 0

Query: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSK+TVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTI 137
           DILQDKWSSAYDVRTI
Sbjct: 121 DILQDKWSSAYDVRTI 136

BLAST of Bhi10G000213 vs. ExPASy TrEMBL
Match: A0A5D3BA03 (Ubiquitin-conjugating enzyme E2 20-like isoform X3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G00870 PE=3 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 2.8e-72
Identity = 135/136 (99.26%), Postives = 135/136 (99.26%), Query Frame = 0

Query: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNT PANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTI 137
           DILQDKWSSAYDVRTI
Sbjct: 121 DILQDKWSSAYDVRTI 136

BLAST of Bhi10G000213 vs. ExPASy TrEMBL
Match: E5GBT5 (Ubiquitin-conjugating enzyme OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 2.8e-72
Identity = 135/136 (99.26%), Postives = 135/136 (99.26%), Query Frame = 0

Query: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNT PANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTI 137
           DILQDKWSSAYDVRTI
Sbjct: 121 DILQDKWSSAYDVRTI 136

BLAST of Bhi10G000213 vs. ExPASy TrEMBL
Match: A0A0A0KF07 (UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G434330 PE=3 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 2.8e-72
Identity = 135/136 (99.26%), Postives = 135/136 (99.26%), Query Frame = 0

Query: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNT PANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTI 137
           DILQDKWSSAYDVRTI
Sbjct: 121 DILQDKWSSAYDVRTI 136

BLAST of Bhi10G000213 vs. ExPASy TrEMBL
Match: A0A1S3BLY8 (ubiquitin-conjugating enzyme E2 20 OS=Cucumis melo OX=3656 GN=LOC103491487 PE=3 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 2.8e-72
Identity = 135/136 (99.26%), Postives = 135/136 (99.26%), Query Frame = 0

Query: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNT PANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTI 137
           DILQDKWSSAYDVRTI
Sbjct: 121 DILQDKWSSAYDVRTI 136

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT1G50490.11.2e-5980.88ubiquitin-conjugating enzyme 20 [more]
AT3G20060.14.5e-5979.56ubiquitin-conjugating enzyme19 [more]
AT3G20060.21.7e-4588.04ubiquitin-conjugating enzyme19 [more]
AT1G14400.12.9e-2145.63ubiquitin carrier protein 1 [more]
AT1G14400.22.9e-2145.63ubiquitin carrier protein 1 [more]
Match NameE-valueIdentityDescription
Q8L7T31.7e-5880.88Ubiquitin-conjugating enzyme E2 20 OS=Arabidopsis thaliana OX=3702 GN=UBC20 PE=2... [more]
Q9LJZ56.4e-5879.56Ubiquitin-conjugating enzyme E2 19 OS=Arabidopsis thaliana OX=3702 GN=UBC19 PE=1... [more]
Q4R9D12.4e-3658.14Ubiquitin-conjugating enzyme E2 C OS=Macaca fascicularis OX=9541 GN=UBE2C PE=2 S... [more]
Q32PA56.9e-3657.36Ubiquitin-conjugating enzyme E2 C OS=Bos taurus OX=9913 GN=UBE2C PE=2 SV=1[more]
O007626.9e-3657.36Ubiquitin-conjugating enzyme E2 C OS=Homo sapiens OX=9606 GN=UBE2C PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_038900928.11.5e-72100.00LOW QUALITY PROTEIN: ubiquitin-conjugating enzyme E2 20 [Benincasa hispida][more]
XP_022957735.13.4e-7299.26ubiquitin-conjugating enzyme E2 20-like [Cucurbita moschata] >KAG6605929.1 Ubiqu... [more]
XP_023533942.14.4e-7298.53ubiquitin-conjugating enzyme E2 20-like [Cucurbita pepo subsp. pepo][more]
ADN33927.15.7e-7299.26ubiquitin-conjugating enzyme [Cucumis melo subsp. melo][more]
XP_004140207.15.7e-7299.26ubiquitin-conjugating enzyme E2 20 [Cucumis sativus] >XP_008449688.1 PREDICTED: ... [more]
Match NameE-valueIdentityDescription
A0A6J1H1C81.6e-7299.26ubiquitin-conjugating enzyme E2 20-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A5D3BA032.8e-7299.26Ubiquitin-conjugating enzyme E2 20-like isoform X3 OS=Cucumis melo var. makuwa O... [more]
E5GBT52.8e-7299.26Ubiquitin-conjugating enzyme OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1[more]
A0A0A0KF072.8e-7299.26UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G434330 PE... [more]
A0A1S3BLY82.8e-7299.26ubiquitin-conjugating enzyme E2 20 OS=Cucumis melo OX=3656 GN=LOC103491487 PE=3 ... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM00212ubc_7coord: 38..157
e-value: 7.3E-30
score: 115.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..19
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..32
NoneNo IPR availablePANTHERPTHR24068UBIQUITIN-CONJUGATING ENZYME E2coord: 19..136
NoneNo IPR availablePANTHERPTHR24068:SF228UBIQUITIN-CONJUGATING ENZYME E2 20coord: 19..136
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3D3.10.110.10Ubiquitin Conjugating Enzymecoord: 28..151
e-value: 7.7E-41
score: 141.3
IPR016135Ubiquitin-conjugating enzyme/RWD-likeSUPERFAMILY54495UBC-likecoord: 28..136
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 39..136
e-value: 4.0E-33
score: 114.0
IPR000608Ubiquitin-conjugating enzyme E2PROSITEPS50127UBC_2coord: 35..162
score: 36.20377
IPR000608Ubiquitin-conjugating enzyme E2CDDcd00195UBCccoord: 38..141
e-value: 1.88394E-44
score: 141.186
IPR023313Ubiquitin-conjugating enzyme, active sitePROSITEPS00183UBC_1coord: 108..123

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi10M000213Bhi10M000213mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031145 anaphase-promoting complex-dependent catabolic process
biological_process GO:0000209 protein polyubiquitination
biological_process GO:0030071 regulation of mitotic metaphase/anaphase transition
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0061631 ubiquitin conjugating enzyme activity