Bhi10G000172 (gene) Wax gourd (B227) v1

Overview


Name	Bhi10G000172
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Pectinesterase
Location	chr10: 5084350 .. 5085493 (+)
RNA-Seq Expression	Bhi10G000172
Synteny	Bhi10G000172

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAAAAGAAAAGGAGCAACAAGCATTAGCAGCTAGACTGAGGGGGACAAAAGCCGCGGTTTACAATTGCAAATTCTTTGGATTTCAAGACACTCTTTGCGACGATGACGGTTTACATCTTCTACAAAATTGCTTCATTCAGGGCACCGTCGACTTCATTTTCGGAAAGGGAACTTCCCTTTATTTGGTATATCTCCAAAATGCTCCTCAAAGAAAACCATATACTATAATATAACATAATGTTTTTCTATATATAGAAATATTTAATTGATAGAACAGTAATAATAATGTAATTTGAAACAATTGCAGAACTGTCAGTTGAATGTGGTGGGAGAGGGAGGATTGGGAGTGATCACAGCCCATTCAAGGGAAGAGGAATCAGACGGCAGTGGATATTCGTTCGTGCATTGTAGCATTACAGGAACGGGTGGCAGAAACACTTATTTGGGAAGAGCTTGGATGCCTAGATCTAGAGTTGTCTTTGCCTATACAACAATGGCTGATATCATGCATGCTGAAGGCTGGCACGACATGGGCCATCATGGTTACGACAAGTAATTACCATATTCTAACTATATATATATATATATATATAGTACTTAGTGCATCTCAAGCTATACCATAATTTTCCACGTGTCAAATGCATTCAAGTAGGATTAAAATTTAAAATGTTAAGATGTAATTTTAGTTCCTATGACTCAAGAAAGTTAAAATTTAGTTTTTATGATTTATAATTAAAATTTAGAATATATGCAATAGTCTCGTAACTATTTATGAGATTTTTATCTAACAATAAATCCACACACTAGATTTTTTGCTTTTAAATCCTAAGGAATAATTTTTAGTTTTTTTTCCAAATCATAAGGACCAAGTTTGTAATTAAATCAATTAAAAATTGTCTTTTCATGTCGCATTCAAGATGCTTTAGAATATATGCAACAGTCGCATTGAATAATGTTTTCTTTTATTTTTATAGAACGGTTTTGTTCGGAGAATACAAGTGTTCGGGACCAGGATCGGAGTCGAGCAGGCGCGTAAATTACGGCAAGCAATTGAGTGATGCAGAAGTGAAGCCGTTCACTAGTCTTGATTACGTGCAGAGCGAGAAGTGGCTGCTTCTCCCACCCAATCCTCAAGTTTAG

mRNA sequence

ATGGGAAAAGAAAAGGAGCAACAAGCATTAGCAGCTAGACTGAGGGGGACAAAAGCCGCGGTTTACAATTGCAAATTCTTTGGATTTCAAGACACTCTTTGCGACGATGACGGTTTACATCTTCTACAAAATTGCTTCATTCAGGGCACCGTCGACTTCATTTTCGGAAAGGGAACTTCCCTTTATTTGAACTGTCAGTTGAATGTGGTGGGAGAGGGAGGATTGGGAGTGATCACAGCCCATTCAAGGGAAGAGGAATCAGACGGCAGTGGATATTCGTTCGTGCATTGTAGCATTACAGGAACGGGTGGCAGAAACACTTATTTGGGAAGAGCTTGGATGCCTAGATCTAGAGTTGTCTTTGCCTATACAACAATGGCTGATATCATGCATGCTGAAGGCTGGCACGACATGGGCCATCATGGTTACGACAAAACGGTTTTGTTCGGAGAATACAAGTGTTCGGGACCAGGATCGGAGTCGAGCAGGCGCGTAAATTACGGCAAGCAATTGAGTGATGCAGAAGTGAAGCCGTTCACTAGTCTTGATTACGTGCAGAGCGAGAAGTGGCTGCTTCTCCCACCCAATCCTCAAGTTTAG

Coding sequence (CDS)

ATGGGAAAAGAAAAGGAGCAACAAGCATTAGCAGCTAGACTGAGGGGGACAAAAGCCGCGGTTTACAATTGCAAATTCTTTGGATTTCAAGACACTCTTTGCGACGATGACGGTTTACATCTTCTACAAAATTGCTTCATTCAGGGCACCGTCGACTTCATTTTCGGAAAGGGAACTTCCCTTTATTTGAACTGTCAGTTGAATGTGGTGGGAGAGGGAGGATTGGGAGTGATCACAGCCCATTCAAGGGAAGAGGAATCAGACGGCAGTGGATATTCGTTCGTGCATTGTAGCATTACAGGAACGGGTGGCAGAAACACTTATTTGGGAAGAGCTTGGATGCCTAGATCTAGAGTTGTCTTTGCCTATACAACAATGGCTGATATCATGCATGCTGAAGGCTGGCACGACATGGGCCATCATGGTTACGACAAAACGGTTTTGTTCGGAGAATACAAGTGTTCGGGACCAGGATCGGAGTCGAGCAGGCGCGTAAATTACGGCAAGCAATTGAGTGATGCAGAAGTGAAGCCGTTCACTAGTCTTGATTACGTGCAGAGCGAGAAGTGGCTGCTTCTCCCACCCAATCCTCAAGTTTAG

Protein sequence

MGKEKEQQALAARLRGTKAAVYNCKFFGFQDTLCDDDGLHLLQNCFIQGTVDFIFGKGTSLYLNCQLNVVGEGGLGVITAHSREEESDGSGYSFVHCSITGTGGRNTYLGRAWMPRSRVVFAYTTMADIMHAEGWHDMGHHGYDKTVLFGEYKCSGPGSESSRRVNYGKQLSDAEVKPFTSLDYVQSEKWLLLPPNPQV

Homology

[Close]

BLAST of Bhi10G000172 vs. TAIR 10
Match: AT5G61680.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 231.5 bits (589), Expect = 5.9e-61
Identity = 106/195 (54.36%), Postives = 137/195 (70.26%), Query Frame = 0


Query: 2   GKEKEQQALAARLRGTKAAVYNCKFFGFQDTLCDDDGLHLLQNCFIQGTVDFIFGKGTSL 61
           GK K  QAL+ R+ G KAA YNCKF+G+QDT+CDD G H  ++C+I+GT DFIFG G SL
Sbjct: 145 GKRKGAQALSMRISGNKAAFYNCKFYGYQDTICDDTGNHFFKDCYIEGTFDFIFGSGRSL 204

Query: 62  YLNCQLNVVGEGGLGVITAHSREEESDGSGYSFVHCSITGTGGRNTYLGRAWMPRSRVVF 121
           YL  QLNVVG+ G+ VITAH+ +  ++ SGYSFVHC +TGT G   YLGR+WM   +VV+
Sbjct: 205 YLGTQLNVVGD-GIRVITAHAGKSAAEKSGYSFVHCKVTGT-GTGIYLGRSWMSHPKVVY 264

Query: 122 AYTTMADIMHAEGWHDMGHHGYDKTVLFGEYKCSGPGSESSRRVNYGKQLSDAEVKPFTS 181
           AYT M+ +++  GW +    G DKTV +GEYKC+G GS   +RV Y + + D E K F S
Sbjct: 265 AYTDMSSVVNPSGWQENREAGRDKTVFYGEYKCTGTGSHKEKRVKYTQDIDDIEAKYFIS 324

Query: 182 LDYVQSEKWLLLPPN 197
           L Y+Q   WLL PP+
Sbjct: 325 LGYIQGSSWLLPPPS 337

[Close]

BLAST of Bhi10G000172 vs. TAIR 10
Match: AT1G69940.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 229.6 bits (584), Expect = 2.3e-60
Identity = 105/194 (54.12%), Postives = 136/194 (70.10%), Query Frame = 0


Query: 2   GKEKEQQALAARLRGTKAAVYNCKFFGFQDTLCDDDGLHLLQNCFIQGTVDFIFGKGTSL 61
           GK K  QAL+ R+ G  AA YNCKF+GFQDT+CDD G H  ++C+++GT DFIFG GTS+
Sbjct: 168 GKTKGAQALSMRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSM 227

Query: 62  YLNCQLNVVGEGGLGVITAHSREEESDGSGYSFVHCSITGTGGRNTYLGRAWMPRSRVVF 121
           YL  QL+VVG+ G+ VI AH+ +   + SGYSFVHC +TGTGG   YLGRAWM   +VV+
Sbjct: 228 YLGTQLHVVGD-GIRVIAAHAGKSAEEKSGYSFVHCKVTGTGG-GIYLGRAWMSHPKVVY 287

Query: 122 AYTTMADIMHAEGWHDMGHHGYDKTVLFGEYKCSGPGSESSRRVNYGKQLSDAEVKPFTS 181
           AYT M  +++  GW +     +DKTV +GEYKCSGPGS  ++RV + + + D E   F S
Sbjct: 288 AYTEMTSVVNPTGWQENKTPAHDKTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANRFLS 347

Query: 182 LDYVQSEKWLLLPP 196
           L Y+Q  KWLL PP
Sbjct: 348 LGYIQGSKWLLPPP 359

[Close]

BLAST of Bhi10G000172 vs. TAIR 10
Match: AT5G07410.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 229.2 bits (583), Expect = 2.9e-60
Identity = 105/194 (54.12%), Postives = 136/194 (70.10%), Query Frame = 0


Query: 2   GKEKEQQALAARLRGTKAAVYNCKFFGFQDTLCDDDGLHLLQNCFIQGTVDFIFGKGTSL 61
           GK K  QAL+ R+ G  AA YNCKF+GFQDT+CDD G H  ++C+++GT DFIFG GTS+
Sbjct: 168 GKTKGAQALSMRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSM 227

Query: 62  YLNCQLNVVGEGGLGVITAHSREEESDGSGYSFVHCSITGTGGRNTYLGRAWMPRSRVVF 121
           YL  QL+VVG+ G+ VI AH+ +   + SGYSFVHC +TGTGG   YLGRAWM   +VV+
Sbjct: 228 YLGTQLHVVGD-GIRVIAAHAGKSAEEKSGYSFVHCKVTGTGG-GIYLGRAWMSHPKVVY 287

Query: 122 AYTTMADIMHAEGWHDMGHHGYDKTVLFGEYKCSGPGSESSRRVNYGKQLSDAEVKPFTS 181
           AYT M  +++  GW +     +DKTV +GEYKCSGPGS  ++RV + + + D E   F S
Sbjct: 288 AYTEMTSVVNPTGWQENKTPAHDKTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANCFLS 347

Query: 182 LDYVQSEKWLLLPP 196
           L Y+Q  KWLL PP
Sbjct: 348 LGYIQGSKWLLPPP 359

[Close]

BLAST of Bhi10G000172 vs. TAIR 10
Match: AT5G07430.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 219.9 bits (559), Expect = 1.8e-57
Identity = 100/188 (53.19%), Postives = 132/188 (70.21%), Query Frame = 0


Query: 8   QALAARLRGTKAAVYNCKFFGFQDTLCDDDGLHLLQNCFIQGTVDFIFGKGTSLYLNCQL 67
           QALA R+   KAA Y+C+F GFQDTLCDD G H  ++C+I+GT DFIFG+G SLYLN QL
Sbjct: 174 QALAMRINADKAAFYSCRFHGFQDTLCDDKGNHFFKDCYIEGTYDFIFGRGASLYLNTQL 233

Query: 68  NVVGEGGLGVITAHSREEESDGSGYSFVHCSITGTGGRNTYLGRAWMPRSRVVFAYTTMA 127
           + VG+ GL VITA  R+  ++ +GY+FVHC +TGT G   YLGR+WM   +VV+A+T M 
Sbjct: 234 HAVGD-GLRVITAQGRQSATEQNGYTFVHCKVTGT-GTGIYLGRSWMSHPKVVYAFTEMT 293

Query: 128 DIMHAEGWHDMGHHGYDKTVLFGEYKCSGPGSESSRRVNYGKQLSDAEVKPFTSLDYVQS 187
            +++  GW +  + GYDKTV +GEYKC GPGS   +RV Y + +   EV PF +L Y++ 
Sbjct: 294 SVVNPSGWRENLNRGYDKTVFYGEYKCFGPGSHLEKRVPYTQDIDKNEVTPFLTLGYIKG 353

Query: 188 EKWLLLPP 196
             WLL PP
Sbjct: 354 STWLLPPP 359

[Close]

BLAST of Bhi10G000172 vs. TAIR 10
Match: AT5G07420.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 219.9 bits (559), Expect = 1.8e-57
Identity = 98/188 (52.13%), Postives = 133/188 (70.74%), Query Frame = 0


Query: 8   QALAARLRGTKAAVYNCKFFGFQDTLCDDDGLHLLQNCFIQGTVDFIFGKGTSLYLNCQL 67
           QALA R+ G KAA YNC+F+GFQDTLCDD G H  +NC+I+GT DFIFG+G SLYL  QL
Sbjct: 174 QALAMRINGDKAAFYNCRFYGFQDTLCDDRGNHFFKNCYIEGTYDFIFGRGASLYLTTQL 233

Query: 68  NVVGEGGLGVITAHSREEESDGSGYSFVHCSITGTGGRNTYLGRAWMPRSRVVFAYTTMA 127
           + VG+ GL VI AH+R+  ++ +GYSFVHC +TG  G   YLGRAWM   +VV++YT M+
Sbjct: 234 HAVGD-GLRVIAAHNRQSTTEQNGYSFVHCKVTGV-GTGIYLGRAWMSHPKVVYSYTEMS 293

Query: 128 DIMHAEGWHDMGHHGYDKTVLFGEYKCSGPGSESSRRVNYGKQLSDAEVKPFTSLDYVQS 187
            +++  GW +     +DKTV +GEY C+GPGS  ++RV + + + + E   F +L Y++ 
Sbjct: 294 SVVNPSGWQENRVRAHDKTVFYGEYMCTGPGSHKAKRVAHTQDIDNKEASQFLTLGYIKG 353

Query: 188 EKWLLLPP 196
            KWLL PP
Sbjct: 354 SKWLLPPP 359

[Close]

BLAST of Bhi10G000172 vs. ExPASy Swiss-Prot
Match: Q9FKF3 (Putative pectinesterase 63 OS=Arabidopsis thaliana OX=3702 GN=PME63 PE=3 SV=2)

HSP 1 Score: 231.5 bits (589), Expect = 8.4e-60
Identity = 106/195 (54.36%), Postives = 137/195 (70.26%), Query Frame = 0


Query: 2   GKEKEQQALAARLRGTKAAVYNCKFFGFQDTLCDDDGLHLLQNCFIQGTVDFIFGKGTSL 61
           GK K  QAL+ R+ G KAA YNCKF+G+QDT+CDD G H  ++C+I+GT DFIFG G SL
Sbjct: 145 GKRKGAQALSMRISGNKAAFYNCKFYGYQDTICDDTGNHFFKDCYIEGTFDFIFGSGRSL 204

Query: 62  YLNCQLNVVGEGGLGVITAHSREEESDGSGYSFVHCSITGTGGRNTYLGRAWMPRSRVVF 121
           YL  QLNVVG+ G+ VITAH+ +  ++ SGYSFVHC +TGT G   YLGR+WM   +VV+
Sbjct: 205 YLGTQLNVVGD-GIRVITAHAGKSAAEKSGYSFVHCKVTGT-GTGIYLGRSWMSHPKVVY 264

Query: 122 AYTTMADIMHAEGWHDMGHHGYDKTVLFGEYKCSGPGSESSRRVNYGKQLSDAEVKPFTS 181
           AYT M+ +++  GW +    G DKTV +GEYKC+G GS   +RV Y + + D E K F S
Sbjct: 265 AYTDMSSVVNPSGWQENREAGRDKTVFYGEYKCTGTGSHKEKRVKYTQDIDDIEAKYFIS 324

Query: 182 LDYVQSEKWLLLPPN 197
           L Y+Q   WLL PP+
Sbjct: 325 LGYIQGSSWLLPPPS 337

[Close]

BLAST of Bhi10G000172 vs. ExPASy Swiss-Prot
Match: Q84WM7 (Pectinesterase PPME1 OS=Arabidopsis thaliana OX=3702 GN=PPME1 PE=1 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 3.2e-59
Identity = 105/194 (54.12%), Postives = 136/194 (70.10%), Query Frame = 0


Query: 2   GKEKEQQALAARLRGTKAAVYNCKFFGFQDTLCDDDGLHLLQNCFIQGTVDFIFGKGTSL 61
           GK K  QAL+ R+ G  AA YNCKF+GFQDT+CDD G H  ++C+++GT DFIFG GTS+
Sbjct: 168 GKTKGAQALSMRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSM 227

Query: 62  YLNCQLNVVGEGGLGVITAHSREEESDGSGYSFVHCSITGTGGRNTYLGRAWMPRSRVVF 121
           YL  QL+VVG+ G+ VI AH+ +   + SGYSFVHC +TGTGG   YLGRAWM   +VV+
Sbjct: 228 YLGTQLHVVGD-GIRVIAAHAGKSAEEKSGYSFVHCKVTGTGG-GIYLGRAWMSHPKVVY 287

Query: 122 AYTTMADIMHAEGWHDMGHHGYDKTVLFGEYKCSGPGSESSRRVNYGKQLSDAEVKPFTS 181
           AYT M  +++  GW +     +DKTV +GEYKCSGPGS  ++RV + + + D E   F S
Sbjct: 288 AYTEMTSVVNPTGWQENKTPAHDKTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANRFLS 347

Query: 182 LDYVQSEKWLLLPP 196
           L Y+Q  KWLL PP
Sbjct: 348 LGYIQGSKWLLPPP 359

[Close]

BLAST of Bhi10G000172 vs. ExPASy Swiss-Prot
Match: Q9LY19 (Probable pectinesterase 48 OS=Arabidopsis thaliana OX=3702 GN=PME48 PE=2 SV=2)

HSP 1 Score: 229.2 bits (583), Expect = 4.2e-59
Identity = 105/194 (54.12%), Postives = 136/194 (70.10%), Query Frame = 0


Query: 2   GKEKEQQALAARLRGTKAAVYNCKFFGFQDTLCDDDGLHLLQNCFIQGTVDFIFGKGTSL 61
           GK K  QAL+ R+ G  AA YNCKF+GFQDT+CDD G H  ++C+++GT DFIFG GTS+
Sbjct: 168 GKTKGAQALSMRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSM 227

Query: 62  YLNCQLNVVGEGGLGVITAHSREEESDGSGYSFVHCSITGTGGRNTYLGRAWMPRSRVVF 121
           YL  QL+VVG+ G+ VI AH+ +   + SGYSFVHC +TGTGG   YLGRAWM   +VV+
Sbjct: 228 YLGTQLHVVGD-GIRVIAAHAGKSAEEKSGYSFVHCKVTGTGG-GIYLGRAWMSHPKVVY 287

Query: 122 AYTTMADIMHAEGWHDMGHHGYDKTVLFGEYKCSGPGSESSRRVNYGKQLSDAEVKPFTS 181
           AYT M  +++  GW +     +DKTV +GEYKCSGPGS  ++RV + + + D E   F S
Sbjct: 288 AYTEMTSVVNPTGWQENKTPAHDKTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANCFLS 347

Query: 182 LDYVQSEKWLLLPP 196
           L Y+Q  KWLL PP
Sbjct: 348 LGYIQGSKWLLPPP 359

[Close]

BLAST of Bhi10G000172 vs. ExPASy Swiss-Prot
Match: Q9LY18 (Probable pectinesterase 49 OS=Arabidopsis thaliana OX=3702 GN=PME49 PE=2 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 2.5e-56
Identity = 98/188 (52.13%), Postives = 133/188 (70.74%), Query Frame = 0


Query: 8   QALAARLRGTKAAVYNCKFFGFQDTLCDDDGLHLLQNCFIQGTVDFIFGKGTSLYLNCQL 67
           QALA R+ G KAA YNC+F+GFQDTLCDD G H  +NC+I+GT DFIFG+G SLYL  QL
Sbjct: 174 QALAMRINGDKAAFYNCRFYGFQDTLCDDRGNHFFKNCYIEGTYDFIFGRGASLYLTTQL 233

Query: 68  NVVGEGGLGVITAHSREEESDGSGYSFVHCSITGTGGRNTYLGRAWMPRSRVVFAYTTMA 127
           + VG+ GL VI AH+R+  ++ +GYSFVHC +TG  G   YLGRAWM   +VV++YT M+
Sbjct: 234 HAVGD-GLRVIAAHNRQSTTEQNGYSFVHCKVTGV-GTGIYLGRAWMSHPKVVYSYTEMS 293

Query: 128 DIMHAEGWHDMGHHGYDKTVLFGEYKCSGPGSESSRRVNYGKQLSDAEVKPFTSLDYVQS 187
            +++  GW +     +DKTV +GEY C+GPGS  ++RV + + + + E   F +L Y++ 
Sbjct: 294 SVVNPSGWQENRVRAHDKTVFYGEYMCTGPGSHKAKRVAHTQDIDNKEASQFLTLGYIKG 353

Query: 188 EKWLLLPP 196
            KWLL PP
Sbjct: 354 SKWLLPPP 359

[Close]

BLAST of Bhi10G000172 vs. ExPASy Swiss-Prot
Match: Q9LY17 (Probable pectinesterase 50 OS=Arabidopsis thaliana OX=3702 GN=PME50 PE=2 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 2.5e-56
Identity = 100/188 (53.19%), Postives = 132/188 (70.21%), Query Frame = 0


Query: 8   QALAARLRGTKAAVYNCKFFGFQDTLCDDDGLHLLQNCFIQGTVDFIFGKGTSLYLNCQL 67
           QALA R+   KAA Y+C+F GFQDTLCDD G H  ++C+I+GT DFIFG+G SLYLN QL
Sbjct: 174 QALAMRINADKAAFYSCRFHGFQDTLCDDKGNHFFKDCYIEGTYDFIFGRGASLYLNTQL 233

Query: 68  NVVGEGGLGVITAHSREEESDGSGYSFVHCSITGTGGRNTYLGRAWMPRSRVVFAYTTMA 127
           + VG+ GL VITA  R+  ++ +GY+FVHC +TGT G   YLGR+WM   +VV+A+T M 
Sbjct: 234 HAVGD-GLRVITAQGRQSATEQNGYTFVHCKVTGT-GTGIYLGRSWMSHPKVVYAFTEMT 293

Query: 128 DIMHAEGWHDMGHHGYDKTVLFGEYKCSGPGSESSRRVNYGKQLSDAEVKPFTSLDYVQS 187
            +++  GW +  + GYDKTV +GEYKC GPGS   +RV Y + +   EV PF +L Y++ 
Sbjct: 294 SVVNPSGWRENLNRGYDKTVFYGEYKCFGPGSHLEKRVPYTQDIDKNEVTPFLTLGYIKG 353

Query: 188 EKWLLLPP 196
             WLL PP
Sbjct: 354 STWLLPPP 359

[Close]

BLAST of Bhi10G000172 vs. NCBI nr
Match: XP_038902684.1 (putative pectinesterase 63 [Benincasa hispida])

HSP 1 Score: 348.2 bits (892), Expect = 4.7e-92
Identity = 164/198 (82.83%), Postives = 176/198 (88.89%), Query Frame = 0


Query: 2   GKEKEQQALAARLRGTKAAVYNCKFFGFQDTLCDDDGLHLLQNCFIQGTVDFIFGKGTSL 61
           G  K  QALAARL GTKAA+YNCKF GFQDTLCDD+GLHL ++CFIQGTVDFIFGKGTSL
Sbjct: 171 GIRKGAQALAARLMGTKAAIYNCKFIGFQDTLCDDEGLHLYKDCFIQGTVDFIFGKGTSL 230

Query: 62  YLNCQLNVVGEGGLGVITAHSREEESDGSGYSFVHCSITGTGGRNTYLGRAWMPRSRVVF 121
           YLN +L+VVG+GGL VITAHSREEESD SGYSF HCSITGTGG+NTYLGRAWMPRSRVVF
Sbjct: 231 YLNSRLDVVGDGGLAVITAHSREEESDSSGYSFAHCSITGTGGKNTYLGRAWMPRSRVVF 290

Query: 122 AYTTMADIMHAEGWHDMGHHGYDKTVLFGEYKCSGPGSESSRRVNYGKQLSDAEVKPFTS 181
           AYTTMADI+HAEGW+DM H GYD TVLFGEYKCSGPG+E S RV Y KQLSD EVKPF S
Sbjct: 291 AYTTMADIIHAEGWNDMNHSGYDATVLFGEYKCSGPGAELSGRVKYSKQLSDVEVKPFIS 350

Query: 182 LDYVQSEKWLLLPPNPQV 200
           L Y+QSEKWLL PPNPQV
Sbjct: 351 LGYLQSEKWLLPPPNPQV 368

[Close]

BLAST of Bhi10G000172 vs. NCBI nr
Match: XP_008449623.1 (PREDICTED: putative pectinesterase 63 [Cucumis melo])

HSP 1 Score: 346.3 bits (887), Expect = 1.8e-91
Identity = 162/198 (81.82%), Postives = 175/198 (88.38%), Query Frame = 0


Query: 2   GKEKEQQALAARLRGTKAAVYNCKFFGFQDTLCDDDGLHLLQNCFIQGTVDFIFGKGTSL 61
           G  K  QALAAR+RG KAA+YNCKF GFQDTLCDDDGLHL ++CFIQGTVDFIFGKGTSL
Sbjct: 172 GVRKGAQALAARIRGNKAAIYNCKFIGFQDTLCDDDGLHLYKDCFIQGTVDFIFGKGTSL 231

Query: 62  YLNCQLNVVGEGGLGVITAHSREEESDGSGYSFVHCSITGTGGRNTYLGRAWMPRSRVVF 121
           YLN QL+VVG GGLGVI AHSR++ESDGSGYSFVHCSITGTGGRNTYLGRAW PRSRVVF
Sbjct: 232 YLNTQLDVVGNGGLGVIAAHSRDQESDGSGYSFVHCSITGTGGRNTYLGRAWRPRSRVVF 291

Query: 122 AYTTMADIMHAEGWHDMGHHGYDKTVLFGEYKCSGPGSESSRRVNYGKQLSDAEVKPFTS 181
           AYTTMADI+H EGW+DM H GYDKTVLFGEYKC GPGS+SS RV Y KQLS  EV+PF S
Sbjct: 292 AYTTMADIIHPEGWNDMNHFGYDKTVLFGEYKCWGPGSKSSERVKYSKQLSYEEVEPFVS 351

Query: 182 LDYVQSEKWLLLPPNPQV 200
           L YVQSEKWLL PPNP++
Sbjct: 352 LGYVQSEKWLLPPPNPEI 369

[Close]

BLAST of Bhi10G000172 vs. NCBI nr
Match: KAA0061750.1 (putative pectinesterase 63 [Cucumis melo var. makuwa] >TYJ96090.1 putative pectinesterase 63 [Cucumis melo var. makuwa])

HSP 1 Score: 346.3 bits (887), Expect = 1.8e-91
Identity = 162/198 (81.82%), Postives = 175/198 (88.38%), Query Frame = 0


Query: 2   GKEKEQQALAARLRGTKAAVYNCKFFGFQDTLCDDDGLHLLQNCFIQGTVDFIFGKGTSL 61
           G  K  QALAAR+RG KAA+YNCKF GFQDTLCDDDGLHL ++CFIQGTVDFIFGKGTSL
Sbjct: 158 GVRKGAQALAARIRGNKAAIYNCKFIGFQDTLCDDDGLHLYKDCFIQGTVDFIFGKGTSL 217

Query: 62  YLNCQLNVVGEGGLGVITAHSREEESDGSGYSFVHCSITGTGGRNTYLGRAWMPRSRVVF 121
           YLN QL+VVG GGLGVI AHSR++ESDGSGYSFVHCSITGTGGRNTYLGRAW PRSRVVF
Sbjct: 218 YLNTQLDVVGNGGLGVIAAHSRDQESDGSGYSFVHCSITGTGGRNTYLGRAWRPRSRVVF 277

Query: 122 AYTTMADIMHAEGWHDMGHHGYDKTVLFGEYKCSGPGSESSRRVNYGKQLSDAEVKPFTS 181
           AYTTMADI+H EGW+DM H GYDKTVLFGEYKC GPGS+SS RV Y KQLS  EV+PF S
Sbjct: 278 AYTTMADIIHPEGWNDMNHFGYDKTVLFGEYKCWGPGSKSSERVKYSKQLSYEEVEPFVS 337

Query: 182 LDYVQSEKWLLLPPNPQV 200
           L YVQSEKWLL PPNP++
Sbjct: 338 LGYVQSEKWLLPPPNPEI 355

[Close]

BLAST of Bhi10G000172 vs. NCBI nr
Match: XP_016900732.1 (PREDICTED: pectinesterase PPME1-like [Cucumis melo])

HSP 1 Score: 345.5 bits (885), Expect = 3.0e-91
Identity = 162/198 (81.82%), Postives = 176/198 (88.89%), Query Frame = 0


Query: 2   GKEKEQQALAARLRGTKAAVYNCKFFGFQDTLCDDDGLHLLQNCFIQGTVDFIFGKGTSL 61
           G  K  QALAAR  GTKAA+YNCKF GFQDTLCDDDGLH  ++CFIQGTVDFIFGKGTSL
Sbjct: 167 GVRKGAQALAARFMGTKAAIYNCKFTGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSL 226

Query: 62  YLNCQLNVVGEGGLGVITAHSREEESDGSGYSFVHCSITGTGGRNTYLGRAWMPRSRVVF 121
           Y+N QL+V G+GGL VITAHSRE+ESDGSGYSFVHCSITGTGG+NT+LGRAWMPRSRVVF
Sbjct: 227 YVNTQLDVAGDGGLAVITAHSREQESDGSGYSFVHCSITGTGGKNTFLGRAWMPRSRVVF 286

Query: 122 AYTTMADIMHAEGWHDMGHHGYDKTVLFGEYKCSGPGSESSRRVNYGKQLSDAEVKPFTS 181
           AYTTMADI+H EGW+DM H G+DKTV+FGEYKCSGPGS S+ RV YGKQLSDAEV PF S
Sbjct: 287 AYTTMADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGSVSTERVPYGKQLSDAEVNPFLS 346

Query: 182 LDYVQSEKWLLLPPNPQV 200
           L YVQSEKWLL PPNPQV
Sbjct: 347 LAYVQSEKWLLPPPNPQV 364

[Close]

BLAST of Bhi10G000172 vs. NCBI nr
Match: KAA0061749.1 (pectinesterase PPME1-like [Cucumis melo var. makuwa] >TYJ96089.1 pectinesterase PPME1-like [Cucumis melo var. makuwa])

HSP 1 Score: 345.5 bits (885), Expect = 3.0e-91
Identity = 162/198 (81.82%), Postives = 176/198 (88.89%), Query Frame = 0


Query: 2   GKEKEQQALAARLRGTKAAVYNCKFFGFQDTLCDDDGLHLLQNCFIQGTVDFIFGKGTSL 61
           G  K  QALAAR  GTKAA+YNCKF GFQDTLCDDDGLH  ++CFIQGTVDFIFGKGTSL
Sbjct: 171 GVRKGAQALAARFMGTKAAIYNCKFTGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSL 230

Query: 62  YLNCQLNVVGEGGLGVITAHSREEESDGSGYSFVHCSITGTGGRNTYLGRAWMPRSRVVF 121
           Y+N QL+V G+GGL VITAHSRE+ESDGSGYSFVHCSITGTGG+NT+LGRAWMPRSRVVF
Sbjct: 231 YVNTQLDVAGDGGLAVITAHSREQESDGSGYSFVHCSITGTGGKNTFLGRAWMPRSRVVF 290

Query: 122 AYTTMADIMHAEGWHDMGHHGYDKTVLFGEYKCSGPGSESSRRVNYGKQLSDAEVKPFTS 181
           AYTTMADI+H EGW+DM H G+DKTV+FGEYKCSGPGS S+ RV YGKQLSDAEV PF S
Sbjct: 291 AYTTMADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGSVSTERVPYGKQLSDAEVNPFLS 350

Query: 182 LDYVQSEKWLLLPPNPQV 200
           L YVQSEKWLL PPNPQV
Sbjct: 351 LAYVQSEKWLLPPPNPQV 368

[Close]

BLAST of Bhi10G000172 vs. ExPASy TrEMBL
Match: A0A5D3B8A8 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G00490 PE=4 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 8.6e-92
Identity = 162/198 (81.82%), Postives = 175/198 (88.38%), Query Frame = 0


Query: 2   GKEKEQQALAARLRGTKAAVYNCKFFGFQDTLCDDDGLHLLQNCFIQGTVDFIFGKGTSL 61
           G  K  QALAAR+RG KAA+YNCKF GFQDTLCDDDGLHL ++CFIQGTVDFIFGKGTSL
Sbjct: 158 GVRKGAQALAARIRGNKAAIYNCKFIGFQDTLCDDDGLHLYKDCFIQGTVDFIFGKGTSL 217

Query: 62  YLNCQLNVVGEGGLGVITAHSREEESDGSGYSFVHCSITGTGGRNTYLGRAWMPRSRVVF 121
           YLN QL+VVG GGLGVI AHSR++ESDGSGYSFVHCSITGTGGRNTYLGRAW PRSRVVF
Sbjct: 218 YLNTQLDVVGNGGLGVIAAHSRDQESDGSGYSFVHCSITGTGGRNTYLGRAWRPRSRVVF 277

Query: 122 AYTTMADIMHAEGWHDMGHHGYDKTVLFGEYKCSGPGSESSRRVNYGKQLSDAEVKPFTS 181
           AYTTMADI+H EGW+DM H GYDKTVLFGEYKC GPGS+SS RV Y KQLS  EV+PF S
Sbjct: 278 AYTTMADIIHPEGWNDMNHFGYDKTVLFGEYKCWGPGSKSSERVKYSKQLSYEEVEPFVS 337

Query: 182 LDYVQSEKWLLLPPNPQV 200
           L YVQSEKWLL PPNP++
Sbjct: 338 LGYVQSEKWLLPPPNPEI 355

[Close]

BLAST of Bhi10G000172 vs. ExPASy TrEMBL
Match: A0A1S3BMG4 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103491453 PE=4 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 8.6e-92
Identity = 162/198 (81.82%), Postives = 175/198 (88.38%), Query Frame = 0


Query: 2   GKEKEQQALAARLRGTKAAVYNCKFFGFQDTLCDDDGLHLLQNCFIQGTVDFIFGKGTSL 61
           G  K  QALAAR+RG KAA+YNCKF GFQDTLCDDDGLHL ++CFIQGTVDFIFGKGTSL
Sbjct: 172 GVRKGAQALAARIRGNKAAIYNCKFIGFQDTLCDDDGLHLYKDCFIQGTVDFIFGKGTSL 231

Query: 62  YLNCQLNVVGEGGLGVITAHSREEESDGSGYSFVHCSITGTGGRNTYLGRAWMPRSRVVF 121
           YLN QL+VVG GGLGVI AHSR++ESDGSGYSFVHCSITGTGGRNTYLGRAW PRSRVVF
Sbjct: 232 YLNTQLDVVGNGGLGVIAAHSRDQESDGSGYSFVHCSITGTGGRNTYLGRAWRPRSRVVF 291

Query: 122 AYTTMADIMHAEGWHDMGHHGYDKTVLFGEYKCSGPGSESSRRVNYGKQLSDAEVKPFTS 181
           AYTTMADI+H EGW+DM H GYDKTVLFGEYKC GPGS+SS RV Y KQLS  EV+PF S
Sbjct: 292 AYTTMADIIHPEGWNDMNHFGYDKTVLFGEYKCWGPGSKSSERVKYSKQLSYEEVEPFVS 351

Query: 182 LDYVQSEKWLLLPPNPQV 200
           L YVQSEKWLL PPNP++
Sbjct: 352 LGYVQSEKWLLPPPNPEI 369

[Close]

BLAST of Bhi10G000172 vs. ExPASy TrEMBL
Match: A0A5A7V7V3 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G00480 PE=4 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 1.5e-91
Identity = 162/198 (81.82%), Postives = 176/198 (88.89%), Query Frame = 0


Query: 2   GKEKEQQALAARLRGTKAAVYNCKFFGFQDTLCDDDGLHLLQNCFIQGTVDFIFGKGTSL 61
           G  K  QALAAR  GTKAA+YNCKF GFQDTLCDDDGLH  ++CFIQGTVDFIFGKGTSL
Sbjct: 171 GVRKGAQALAARFMGTKAAIYNCKFTGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSL 230

Query: 62  YLNCQLNVVGEGGLGVITAHSREEESDGSGYSFVHCSITGTGGRNTYLGRAWMPRSRVVF 121
           Y+N QL+V G+GGL VITAHSRE+ESDGSGYSFVHCSITGTGG+NT+LGRAWMPRSRVVF
Sbjct: 231 YVNTQLDVAGDGGLAVITAHSREQESDGSGYSFVHCSITGTGGKNTFLGRAWMPRSRVVF 290

Query: 122 AYTTMADIMHAEGWHDMGHHGYDKTVLFGEYKCSGPGSESSRRVNYGKQLSDAEVKPFTS 181
           AYTTMADI+H EGW+DM H G+DKTV+FGEYKCSGPGS S+ RV YGKQLSDAEV PF S
Sbjct: 291 AYTTMADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGSVSTERVPYGKQLSDAEVNPFLS 350

Query: 182 LDYVQSEKWLLLPPNPQV 200
           L YVQSEKWLL PPNPQV
Sbjct: 351 LAYVQSEKWLLPPPNPQV 368

[Close]

BLAST of Bhi10G000172 vs. ExPASy TrEMBL
Match: A0A1S4DXM9 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103491454 PE=4 SV=1)

HSP 1 Score: 345.5 bits (885), Expect = 1.5e-91
Identity = 162/198 (81.82%), Postives = 176/198 (88.89%), Query Frame = 0


Query: 2   GKEKEQQALAARLRGTKAAVYNCKFFGFQDTLCDDDGLHLLQNCFIQGTVDFIFGKGTSL 61
           G  K  QALAAR  GTKAA+YNCKF GFQDTLCDDDGLH  ++CFIQGTVDFIFGKGTSL
Sbjct: 167 GVRKGAQALAARFMGTKAAIYNCKFTGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSL 226

Query: 62  YLNCQLNVVGEGGLGVITAHSREEESDGSGYSFVHCSITGTGGRNTYLGRAWMPRSRVVF 121
           Y+N QL+V G+GGL VITAHSRE+ESDGSGYSFVHCSITGTGG+NT+LGRAWMPRSRVVF
Sbjct: 227 YVNTQLDVAGDGGLAVITAHSREQESDGSGYSFVHCSITGTGGKNTFLGRAWMPRSRVVF 286

Query: 122 AYTTMADIMHAEGWHDMGHHGYDKTVLFGEYKCSGPGSESSRRVNYGKQLSDAEVKPFTS 181
           AYTTMADI+H EGW+DM H G+DKTV+FGEYKCSGPGS S+ RV YGKQLSDAEV PF S
Sbjct: 287 AYTTMADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGSVSTERVPYGKQLSDAEVNPFLS 346

Query: 182 LDYVQSEKWLLLPPNPQV 200
           L YVQSEKWLL PPNPQV
Sbjct: 347 LAYVQSEKWLLPPPNPQV 364

[Close]

BLAST of Bhi10G000172 vs. ExPASy TrEMBL
Match: A0A0A0KEH2 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G428030 PE=4 SV=1)

HSP 1 Score: 338.6 bits (867), Expect = 1.8e-89
Identity = 157/198 (79.29%), Postives = 175/198 (88.38%), Query Frame = 0


Query: 2   GKEKEQQALAARLRGTKAAVYNCKFFGFQDTLCDDDGLHLLQNCFIQGTVDFIFGKGTSL 61
           G  K  QALAAR  GTKAA+YNCKF GFQDTLCDDDGLH  ++CFIQGTVDFIFGKGTSL
Sbjct: 173 GVRKGAQALAARFMGTKAAIYNCKFLGFQDTLCDDDGLHFYKDCFIQGTVDFIFGKGTSL 232

Query: 62  YLNCQLNVVGEGGLGVITAHSREEESDGSGYSFVHCSITGTGGRNTYLGRAWMPRSRVVF 121
           YLN QL+V G+GGL VITAHSRE+E+D SGYSFVHCSITGTGG+NTYLGRAWMPRSRVVF
Sbjct: 233 YLNTQLDVAGDGGLAVITAHSREQEADTSGYSFVHCSITGTGGKNTYLGRAWMPRSRVVF 292

Query: 122 AYTTMADIMHAEGWHDMGHHGYDKTVLFGEYKCSGPGSESSRRVNYGKQLSDAEVKPFTS 181
           AYTT+ADI+H EGW+DM H G+DKTV+FGEYKCSGPG+ S+ RV YGKQL+D EVKPF  
Sbjct: 293 AYTTIADIIHPEGWNDMKHAGFDKTVMFGEYKCSGPGAVSTGRVAYGKQLTDVEVKPFLG 352

Query: 182 LDYVQSEKWLLLPPNPQV 200
           L+YVQSEKWLL PP+PQV
Sbjct: 353 LEYVQSEKWLLPPPSPQV 370

The following BLAST results are available for this feature:

BLAST of Bhi10G000172 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G61680.1	5.9e-61	54.36	Pectin lyase-like superfamily protein	[more]
AT1G69940.1	2.3e-60	54.12	Pectin lyase-like superfamily protein	[more]
AT5G07410.1	2.9e-60	54.12	Pectin lyase-like superfamily protein	[more]
AT5G07430.1	1.8e-57	53.19	Pectin lyase-like superfamily protein	[more]
AT5G07420.1	1.8e-57	52.13	Pectin lyase-like superfamily protein	[more]

BLAST of Bhi10G000172 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FKF3	8.4e-60	54.36	Putative pectinesterase 63 OS=Arabidopsis thaliana OX=3702 GN=PME63 PE=3 SV=2	[more]
Q84WM7	3.2e-59	54.12	Pectinesterase PPME1 OS=Arabidopsis thaliana OX=3702 GN=PPME1 PE=1 SV=1	[more]
Q9LY19	4.2e-59	54.12	Probable pectinesterase 48 OS=Arabidopsis thaliana OX=3702 GN=PME48 PE=2 SV=2	[more]
Q9LY18	2.5e-56	52.13	Probable pectinesterase 49 OS=Arabidopsis thaliana OX=3702 GN=PME49 PE=2 SV=1	[more]
Q9LY17	2.5e-56	53.19	Probable pectinesterase 50 OS=Arabidopsis thaliana OX=3702 GN=PME50 PE=2 SV=1	[more]

BLAST of Bhi10G000172 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038902684.1	4.7e-92	82.83	putative pectinesterase 63 [Benincasa hispida]	[more]
XP_008449623.1	1.8e-91	81.82	PREDICTED: putative pectinesterase 63 [Cucumis melo]	[more]
KAA0061750.1	1.8e-91	81.82	putative pectinesterase 63 [Cucumis melo var. makuwa] >TYJ96090.1 putative pecti...	[more]
XP_016900732.1	3.0e-91	81.82	PREDICTED: pectinesterase PPME1-like [Cucumis melo]	[more]
KAA0061749.1	3.0e-91	81.82	pectinesterase PPME1-like [Cucumis melo var. makuwa] >TYJ96089.1 pectinesterase ...	[more]

BLAST of Bhi10G000172 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3B8A8	8.6e-92	81.82	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G00490...	[more]
A0A1S3BMG4	8.6e-92	81.82	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103491453 PE=4 SV=1	[more]
A0A5A7V7V3	1.5e-91	81.82	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G00480...	[more]
A0A1S4DXM9	1.5e-91	81.82	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103491454 PE=4 SV=1	[more]
A0A0A0KEH2	1.8e-89	79.29	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_6G428030 PE=4 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 5..187 e-value: 1.2E-37 score: 129.2
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 2..194 e-value: 3.1E-64 score: 219.4
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 3..195
None	No IPR available	PANTHER	PTHR31321:SF30	PECTINESTERASE	coord: 3..195
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 47..56
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 6..191

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi10M000172	Bhi10M000172	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0045490	pectin catabolic process
biological_process	GO:0006468	protein phosphorylation
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0004672	protein kinase activity