Bhi09G002793 (gene) Wax gourd (B227) v1

Overview


Name	Bhi09G002793
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	E3 ubiquitin-protein ligase RMA3
Location	chr9: 86503704 .. 86506572 (-)
RNA-Seq Expression	Bhi09G002793
Synteny	Bhi09G002793

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CACGCGCTTACCTTTCTCGTGAAGTACACGCGCGAATTCCCTCATTTCTATATAACTCCCTTTTTTCGCATCGTTAGTCTCTCCATCTCTCTAGAATTGTTCTTGAAATTCTCTCCGTCGAACATTTTCTTCATTTAATTTCGGTCGGAGATTGTCGTACGAAACCCTTGAGGTTCTTATTTAACGGTGAGTTTTTTTTTTAATGTTTTTAATTTCCAAGCTTACTTTCGGTTTGATTATTGAATTGTGTTATGATTTTGAAGGAAACGTGTTAAGGGAATAGGGGTTTTGTTTGAATGAATGGATATTTCTTACTTTCTAAGAAAACGAGTGTTGGATCTCAATTCATGCGTTGTTGGGCGTTGGGTGAATTTGAACTGCTGCCCTTTTCTTCTTTTGATGTGATTCGATCTAGGTTGATTTTGGGGGGTTCTGTTTCATTTAAACTGCATGATTTTGCGGAGAAATTTTATGTCTGTGGCGTTTGGTTTGTTGCCTTTTTTTTTTTTTTTTTTTTTTCTACCTCTAGATTTTCAACTTTGAAGTTTCGAGATGATTTGGGGGCTTCGAGATTGATTCTGAAGACTTGCTTCCATTCATAACCGTTTCTCTCTGTTCACCAATTTAGAGGATAAGTAGTGTAAACTACAAGCTGAAGGGGTCGATATTTTTTTTATAATTGTTATTTATTTGGGATTTTTGCATTGTTGGTTGTTCTGCAATTGGTTTGCTAGCTTATGTGCTTCTCACGTTTCCCTCCCAAAAAAATATTCATCTTTGTCTCTTTCCTTAATCCTTATAGAGGGTGTTCTGAGCCACGATTGTGGTTTGTGACAGTTGATTTCTGAATGGTCTGAATATTTTGTATCTACATTTATTGCTTGAGGCTTTCTTTTGAAATTTCTAAATGTAGCTACAAGTCATTGGTATTTTACCATTCAACATGATCCCGAGTTTTATGACTACAAATCGACTCTTGAATCTTTTGAGTTTTCACGATCCTTTTGACCTCTTTGCTATAAATTGGCTTGGGTTGGTTGTGGACAGAACAGGGCTGAAGTTTTGCATATAATGTGTGATGAAATGTTGTTCTTAGTCCACCATTTGGTTTTTGGTTTTGAAAATCGTGTTTGTTCTCTCACATAGGTTTTCATGTTCTTCAAGGGAACATTTGGATTTATTGCCAAATTTCAAAAACAAAAACAAGTTTTCAAAAGCTACTTTTTTATTATTTTTATTTTTTATTTTTTATTTTTATTTTTTTTTAGTTTTCAAAACTTAGCTTGGGTTTTGAAAACATTCCTAAAAAGTACGATAACAAAATAAGTGGAAGTATTGTTTAGGGTTTAGTTTCTACAAATTTCAAGGTAGAAGAAAAGCTTCACACATGCTAAGAAGACACTTTCATGACACAAATTGCGAATGGAAGAAGAATTAGATGAAGGACGTTCACCCCAGAGGTTAATCAAGAGCGCTGGGCTATAACCTCCTTGGTGAAAGTCCTTCATCTGATTCTTCTTCCATTTGCAATTTGTTTCATGAAAGTGTCTTCTTAGCTTGTGTGAAGCTTCTTTTCTACCTTGAAATTTGTTTTGCTCTTTTGCAATCCTATTTTATTGGGAAAACAACTGGTATAAGAGGCAATTTTATGGTATTTGCATTATTGCCAATGGAGCATTGTTATTTATGTGCTCAAACTGTTATCTGTCTATAATGTTCTCTTGATTTTATTCTCTAGTTTACTCATTTTTCAAGAATCTCATCATCTCAATGTATCTTTTCGTAAACAGAACGCATTACTCTTTCTATGGAACAAAATTATTCTGTACCTCACTCTGTATCTGAGGCATACTTAGAGTCTGAACAAGATGTCTCTCTCAAGCAAAACTGGAAATCTATCTCAACTCAGTCAACAGTCTCAGAAGACGCAAATGGTTGCTTCGATTGCAACATTTGCTTAGACTCAGCAGCTGAACCTGTTGTCACCCTCTGTGGTCACCTCTACTGCTGGCCGTGTATTTACAAATGGCTTCATGTTCAAATCTCCAGCAACAGACCCGAAAACACCCAGAACTGCCCTGTTTGTAAGGCTAGCATTACTCCCTCCTCTTTGGTTCCCCTATATGGTCGTGGCACATCAAACTCAGATTCCGAATCCAAGAAGTCTCACTTGGGTATGGTTGTACCTCGAAGACCACCACCCTCAATGAACACACCGCCCCATTCCAATGCAACCTCTGCATTGCATCCAAGCCAGGAGCTTCATCCAAATTATGTCCGCTCGCCCTCACACCCAATCTATCACCAACAATATTTCCCCCAGGCCTACGGTGACTTTGCTTCATACGCGCCGTCTTATCTCGGTAATGCCGTGATAACGAGTCTGCTAAACCCGACAATTGGGATGTTTGGAGAAACAGTTTTCACTAGAATTTTTGGTAGCGTGGATGGGAACTTATTGCCATACCCACCATACAACAACGCCATCCCTGGAAATGCCAGTACCAGAATGAGAAGACAGGAAATGCAGCTTGATAAGTCTCTTAATAGAGTTTCCATCTTTCTATTTTGCTGCTTTATTATCTGTCTTCTGTTATTTTGAAATTGGGAATAGATGTTGTGTGGAGAAGCATGTGATATACAGATAAACAACTAGAATTTTGTTTGATGGCTTTGCTGTGAGAAGTCATCTATATGTACAGAAGGTTAACCTAATATCACATATTATTTTGTAATTTCTTTTGGTGCTTGTGTGTGTGCCGTGTTTATAGTACTTCCCTCTGTTTTCCCACACTCTAGCAGTCTAATCTGTTTTCTTTCCTTAGAAATTGATTGACATTGGTATATAAATAAATGATATTATGTTGTA

mRNA sequence

CACGCGCTTACCTTTCTCGTGAAGTACACGCGCGAATTCCCTCATTTCTATATAACTCCCTTTTTTCGCATCGTTAGTCTCTCCATCTCTCTAGAATTGTTCTTGAAATTCTCTCCGTCGAACATTTTCTTCATTTAATTTCGGTCGGAGATTGTCGTACGAAACCCTTGAGGTTCTTATTTAACGAACGCATTACTCTTTCTATGGAACAAAATTATTCTGTACCTCACTCTGTATCTGAGGCATACTTAGAGTCTGAACAAGATGTCTCTCTCAAGCAAAACTGGAAATCTATCTCAACTCAGTCAACAGTCTCAGAAGACGCAAATGGTTGCTTCGATTGCAACATTTGCTTAGACTCAGCAGCTGAACCTGTTGTCACCCTCTGTGGTCACCTCTACTGCTGGCCGTGTATTTACAAATGGCTTCATGTTCAAATCTCCAGCAACAGACCCGAAAACACCCAGAACTGCCCTGTTTGTAAGGCTAGCATTACTCCCTCCTCTTTGGTTCCCCTATATGGTCGTGGCACATCAAACTCAGATTCCGAATCCAAGAAGTCTCACTTGGGTATGGTTGTACCTCGAAGACCACCACCCTCAATGAACACACCGCCCCATTCCAATGCAACCTCTGCATTGCATCCAAGCCAGGAGCTTCATCCAAATTATGTCCGCTCGCCCTCACACCCAATCTATCACCAACAATATTTCCCCCAGGCCTACGGTGACTTTGCTTCATACGCGCCGTCTTATCTCGGTAATGCCGTGATAACGAGTCTGCTAAACCCGACAATTGGGATGTTTGGAGAAACAGTTTTCACTAGAATTTTTGGTAGCGTGGATGGGAACTTATTGCCATACCCACCATACAACAACGCCATCCCTGGAAATGCCAGTACCAGAATGAGAAGACAGGAAATGCAGCTTGATAAGTCTCTTAATAGAGTTTCCATCTTTCTATTTTGCTGCTTTATTATCTGTCTTCTGTTATTTTGAAATTGGGAATAGATGTTGTGTGGAGAAGCATGTGATATACAGATAAACAACTAGAATTTTGTTTGATGGCTTTGCTGTGAGAAGTCATCTATATGTACAGAAGGTTAACCTAATATCACATATTATTTTGTAATTTCTTTTGGTGCTTGTGTGTGTGCCGTGTTTATAGTACTTCCCTCTGTTTTCCCACACTCTAGCAGTCTAATCTGTTTTCTTTCCTTAGAAATTGATTGACATTGGTATATAAATAAATGATATTATGTTGTA

Coding sequence (CDS)

ATGGAACAAAATTATTCTGTACCTCACTCTGTATCTGAGGCATACTTAGAGTCTGAACAAGATGTCTCTCTCAAGCAAAACTGGAAATCTATCTCAACTCAGTCAACAGTCTCAGAAGACGCAAATGGTTGCTTCGATTGCAACATTTGCTTAGACTCAGCAGCTGAACCTGTTGTCACCCTCTGTGGTCACCTCTACTGCTGGCCGTGTATTTACAAATGGCTTCATGTTCAAATCTCCAGCAACAGACCCGAAAACACCCAGAACTGCCCTGTTTGTAAGGCTAGCATTACTCCCTCCTCTTTGGTTCCCCTATATGGTCGTGGCACATCAAACTCAGATTCCGAATCCAAGAAGTCTCACTTGGGTATGGTTGTACCTCGAAGACCACCACCCTCAATGAACACACCGCCCCATTCCAATGCAACCTCTGCATTGCATCCAAGCCAGGAGCTTCATCCAAATTATGTCCGCTCGCCCTCACACCCAATCTATCACCAACAATATTTCCCCCAGGCCTACGGTGACTTTGCTTCATACGCGCCGTCTTATCTCGGTAATGCCGTGATAACGAGTCTGCTAAACCCGACAATTGGGATGTTTGGAGAAACAGTTTTCACTAGAATTTTTGGTAGCGTGGATGGGAACTTATTGCCATACCCACCATACAACAACGCCATCCCTGGAAATGCCAGTACCAGAATGAGAAGACAGGAAATGCAGCTTGATAAGTCTCTTAATAGAGTTTCCATCTTTCTATTTTGCTGCTTTATTATCTGTCTTCTGTTATTTTGA

Protein sequence

MEQNYSVPHSVSEAYLESEQDVSLKQNWKSISTQSTVSEDANGCFDCNICLDSAAEPVVTLCGHLYCWPCIYKWLHVQISSNRPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGMVVPRRPPPSMNTPPHSNATSALHPSQELHPNYVRSPSHPIYHQQYFPQAYGDFASYAPSYLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNNAIPGNASTRMRRQEMQLDKSLNRVSIFLFCCFIICLLLF

Homology

[Close]

BLAST of Bhi09G002793 vs. TAIR 10
Match: AT4G27470.1 (RING membrane-anchor 3 )

HSP 1 Score: 193.4 bits (490), Expect = 2.4e-49
Identity = 123/268 (45.90%), Postives = 162/268 (60.45%), Query Frame = 0


Query: 1   MEQNYSVPHSVSEAYLESEQDVSLKQNWKSISTQSTVSEDANGCFDCNICLDSAAEPVVT 60
           ME N+ +    S+A    +     KQ     +  +    + +GCFDCNICLD+A +PVVT
Sbjct: 1   MEGNFFIR---SDAQRAHDNGFIAKQKPNLTTAPTAGQANESGCFDCNICLDTAHDPVVT 60

Query: 61  LCGHLYCWPCIYKWLHVQISS-NRPENTQNCPVCKASITPSSLVPLYGRGTSNSDS---E 120
           LCGHL+CWPCIYKWLHVQ+SS +  ++  NCPVCK++IT +SLVPLYGRG S+  S    
Sbjct: 61  LCGHLFCWPCIYKWLHVQLSSVSVDQHQNNCPVCKSNITITSLVPLYGRGMSSPSSTFGS 120

Query: 121 SKKSHLGMVVPRRPPPSMNTPPHSNATSALHPSQELHPNYVRSPSHPIYHQQYFPQAYGD 180
            K+  L   +PRRP PS    P ++A S+L+PS +   +   SPS   ++ QY P+    
Sbjct: 121 KKQDALSTDIPRRPAPSALRNPITSA-SSLNPSLQ---HQTLSPS--FHNHQYSPRG--- 180

Query: 181 FASYAPSYLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNNAIPGNASTRMR 240
           F +   + L NAV+ S L P IGMFG+ V+TRIFG+    +    PY        S RM 
Sbjct: 181 FTTTESTDLANAVMMSFLYPVIGMFGDLVYTRIFGTFTNTIA--QPYQ-------SQRM- 240

Query: 241 RQEMQLDKSLNRVSIFLFCCFIICLLLF 265
              MQ +KSLNRVSIF  CC I+CLLLF
Sbjct: 241 ---MQREKSLNRVSIFFLCCIILCLLLF 243

[Close]

BLAST of Bhi09G002793 vs. TAIR 10
Match: AT4G03510.1 (RING membrane-anchor 1 )

HSP 1 Score: 163.3 bits (412), Expect = 2.6e-40
Identity = 103/240 (42.92%), Postives = 143/240 (59.58%), Query Frame = 0


Query: 30  SISTQSTVSEDANGCFDCNICLDSAAEPVVTLCGHLYCWPCIYKWLHVQ--ISSNRPENT 89
           ++S  S  ++D+N  FDCNICLDS  EPVVTLCGHL+CWPCI+KWL VQ   +S+  +  
Sbjct: 33  TVSVPSDDTDDSN--FDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRH 92

Query: 90  QNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGMVVPRRPPP---SMNTPPHSNATS 149
           + CPVCK+ ++ S+LVPLYGRG   +  E K S     VP+RP      +  P    A++
Sbjct: 93  RQCPVCKSKVSHSTLVPLYGRGRCTTQEEGKNS-----VPKRPVGPVYRLEMPNSPYAST 152

Query: 150 ALHPSQELHPNYVRSPSHPIYHQQYFPQAYGDFASYAPSYLGNAVITSLLNPTIGMFGET 209
            L  SQ +H N   SP      + Y+P + G  +S + SY      +++L+P + M GE 
Sbjct: 153 DLRLSQRVHFN---SP-----QEGYYPVS-GVMSSNSLSY------SAVLDPVMVMVGEM 212

Query: 210 VFTRIFGSVDGNLLPYPPYNNAIPGNASTRMRRQEMQLDKSLNRVSIFLFCCFIICLLLF 265
           V TR+FG+   +   YP   N + G +  RMRR+ MQ DKSL R+  F  CC ++CLLLF
Sbjct: 213 VATRLFGTRVMDRFAYPDTYN-LAGTSGPRMRRRIMQADKSLGRIFFFFMCCVVLCLLLF 249

[Close]

BLAST of Bhi09G002793 vs. TAIR 10
Match: AT4G03510.2 (RING membrane-anchor 1 )

HSP 1 Score: 163.3 bits (412), Expect = 2.6e-40
Identity = 103/240 (42.92%), Postives = 143/240 (59.58%), Query Frame = 0


Query: 30  SISTQSTVSEDANGCFDCNICLDSAAEPVVTLCGHLYCWPCIYKWLHVQ--ISSNRPENT 89
           ++S  S  ++D+N  FDCNICLDS  EPVVTLCGHL+CWPCI+KWL VQ   +S+  +  
Sbjct: 33  TVSVPSDDTDDSN--FDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRH 92

Query: 90  QNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGMVVPRRPPP---SMNTPPHSNATS 149
           + CPVCK+ ++ S+LVPLYGRG   +  E K S     VP+RP      +  P    A++
Sbjct: 93  RQCPVCKSKVSHSTLVPLYGRGRCTTQEEGKNS-----VPKRPVGPVYRLEMPNSPYAST 152

Query: 150 ALHPSQELHPNYVRSPSHPIYHQQYFPQAYGDFASYAPSYLGNAVITSLLNPTIGMFGET 209
            L  SQ +H N   SP      + Y+P + G  +S + SY      +++L+P + M GE 
Sbjct: 153 DLRLSQRVHFN---SP-----QEGYYPVS-GVMSSNSLSY------SAVLDPVMVMVGEM 212

Query: 210 VFTRIFGSVDGNLLPYPPYNNAIPGNASTRMRRQEMQLDKSLNRVSIFLFCCFIICLLLF 265
           V TR+FG+   +   YP   N + G +  RMRR+ MQ DKSL R+  F  CC ++CLLLF
Sbjct: 213 VATRLFGTRVMDRFAYPDTYN-LAGTSGPRMRRRIMQADKSLGRIFFFFMCCVVLCLLLF 249

[Close]

BLAST of Bhi09G002793 vs. TAIR 10
Match: AT4G28270.1 (RING membrane-anchor 2 )

HSP 1 Score: 126.3 bits (316), Expect = 3.6e-29
Identity = 79/236 (33.47%), Postives = 109/236 (46.19%), Query Frame = 0


Query: 36  TVSEDANGCFDCNICLDSAAEPVVTLCGHLYCWPCIYKWLHVQISS-------NRPENTQ 95
           T   D+ G FDCNICLD   +PVVTLCGHL+CWPCI+KW +   +S       +      
Sbjct: 10  TTLVDSGGDFDCNICLDQVRDPVVTLCGHLFCWPCIHKWTYASNNSRQRVDQYDHKREPP 69

Query: 96  NCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGMVVPRRPPPSMNTPPHSNATSALHP 155
            CPVCK+ ++ ++LVP+YGRG        K    G  VP R                   
Sbjct: 70  KCPVCKSDVSEATLVPIYGRG-------QKAPQSGSNVPSR------------------- 129

Query: 156 SQELHPNYVRSPSHPIYHQQYFPQAYGDFASYAPSYLGNAVITSLLNPTIGMFGETVFTR 215
                      P+ P+Y  +   Q  G+  S    Y        + +P +G+  E V+ R
Sbjct: 130 -----------PTGPVYDLRGVGQRLGEGESQRYMY-------RMPDPVMGVVCEMVYRR 189

Query: 216 IFGSVDGNLLPYPPYNNAIPGNASTRMRRQEMQLDKSLNRVSIFLFCCFIICLLLF 265
           +FG    N+ PY   N         R RR+ MQ ++SL+RV +FL C   +CL LF
Sbjct: 190 LFGESSSNMAPYRDMN--------VRSRRRAMQAEESLSRVYLFLLCFMFMCLFLF 193

[Close]

BLAST of Bhi09G002793 vs. TAIR 10
Match: AT1G74990.1 (RING/U-box superfamily protein )

HSP 1 Score: 113.2 bits (282), Expect = 3.1e-25
Identity = 53/101 (52.48%), Postives = 68/101 (67.33%), Query Frame = 0


Query: 30  SISTQSTVSEDANGCFDCNICLDSAAEPVVTLCGHLYCWPCIYKWLHVQISSNRPENTQN 89
           +I+T +   +DA+  F CNICL+ A EP+VTLCGHL+CWPC+YKWLH    SN      +
Sbjct: 2   AINTITNEEDDASNNFGCNICLELAREPIVTLCGHLFCWPCLYKWLHYHSKSN------H 61

Query: 90  CPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGMVVPRRP 131
           CPVCKA +   +LVPLYG G  +SD  SK +  G+ VP RP
Sbjct: 62  CPVCKALVKEDTLVPLYGMGKPSSDPRSKLNS-GVTVPNRP 95

[Close]

BLAST of Bhi09G002793 vs. ExPASy Swiss-Prot
Match: Q8GUK7 (E3 ubiquitin-protein ligase RMA3 OS=Arabidopsis thaliana OX=3702 GN=RMA3 PE=1 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 3.3e-48
Identity = 123/268 (45.90%), Postives = 162/268 (60.45%), Query Frame = 0


Query: 1   MEQNYSVPHSVSEAYLESEQDVSLKQNWKSISTQSTVSEDANGCFDCNICLDSAAEPVVT 60
           ME N+ +    S+A    +     KQ     +  +    + +GCFDCNICLD+A +PVVT
Sbjct: 1   MEGNFFIR---SDAQRAHDNGFIAKQKPNLTTAPTAGQANESGCFDCNICLDTAHDPVVT 60

Query: 61  LCGHLYCWPCIYKWLHVQISS-NRPENTQNCPVCKASITPSSLVPLYGRGTSNSDS---E 120
           LCGHL+CWPCIYKWLHVQ+SS +  ++  NCPVCK++IT +SLVPLYGRG S+  S    
Sbjct: 61  LCGHLFCWPCIYKWLHVQLSSVSVDQHQNNCPVCKSNITITSLVPLYGRGMSSPSSTFGS 120

Query: 121 SKKSHLGMVVPRRPPPSMNTPPHSNATSALHPSQELHPNYVRSPSHPIYHQQYFPQAYGD 180
            K+  L   +PRRP PS    P ++A S+L+PS +   +   SPS   ++ QY P+    
Sbjct: 121 KKQDALSTDIPRRPAPSALRNPITSA-SSLNPSLQ---HQTLSPS--FHNHQYSPRG--- 180

Query: 181 FASYAPSYLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNNAIPGNASTRMR 240
           F +   + L NAV+ S L P IGMFG+ V+TRIFG+    +    PY        S RM 
Sbjct: 181 FTTTESTDLANAVMMSFLYPVIGMFGDLVYTRIFGTFTNTIA--QPYQ-------SQRM- 240

Query: 241 RQEMQLDKSLNRVSIFLFCCFIICLLLF 265
              MQ +KSLNRVSIF  CC I+CLLLF
Sbjct: 241 ---MQREKSLNRVSIFFLCCIILCLLLF 243

[Close]

BLAST of Bhi09G002793 vs. ExPASy Swiss-Prot
Match: Q6R567 (E3 ubiquitin-protein ligase RMA1H1 OS=Capsicum annuum OX=4072 GN=RMA1H1 PE=1 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 1.9e-43
Identity = 106/241 (43.98%), Postives = 137/241 (56.85%), Query Frame = 0


Query: 28  WKSISTQSTVSEDANGCFDCNICLDSAAEPVVTLCGHLYCWPCIYKWLHVQ--ISSNRPE 87
           WKS++ +  V E+ +G FDCNICLD   EPV+TLCGHLYCWPCIYKW++ Q   S N  +
Sbjct: 24  WKSMNDE--VEENISGGFDCNICLDCVHEPVITLCGHLYCWPCIYKWIYFQSVSSENSDQ 83

Query: 88  NTQNCPVCKASITPSSLVPLYGR-GTSNSDSESKKSHLGMVVPRRPPPSMNTPPHSNATS 147
               CPVCKA ++  +L+PLYGR G S   SE K  +LG+V+P+RPP           T+
Sbjct: 84  QQPQCPVCKAEVSEKTLIPLYGRGGQSTKPSEGKAPNLGIVIPQRPPSPRCGGHFLLPTT 143

Query: 148 ALHPSQELHPNYVRSPSHPIYHQQYFPQAYGDFASYAPSYLGNAVITSLLNPTIGMFGET 207
             +PSQ L     +  S     Q   P   G + S      G A    L +    M GE 
Sbjct: 144 DSNPSQLLQRRGYQQQS-----QTRQPAYQGSYMSSPMLSPGGATANMLQH---SMIGEV 203

Query: 208 VFTRIFGSVDGNLLPYP-PYNNAIPGNASTRMRRQEMQLDKSLNRVSIFLFCCFIICLLL 265
            + RIFG+    +  YP  YN AI  ++S RMRRQ  Q D+SL R+  FLFCCF+ CL+L
Sbjct: 204 AYARIFGNSSTTMYTYPNSYNLAI--SSSPRMRRQLSQADRSLGRICFFLFCCFVTCLIL 252

[Close]

BLAST of Bhi09G002793 vs. ExPASy Swiss-Prot
Match: O64425 (E3 ubiquitin-protein ligase RMA1 OS=Arabidopsis thaliana OX=3702 GN=RMA1 PE=1 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 3.7e-39
Identity = 103/240 (42.92%), Postives = 143/240 (59.58%), Query Frame = 0


Query: 30  SISTQSTVSEDANGCFDCNICLDSAAEPVVTLCGHLYCWPCIYKWLHVQ--ISSNRPENT 89
           ++S  S  ++D+N  FDCNICLDS  EPVVTLCGHL+CWPCI+KWL VQ   +S+  +  
Sbjct: 33  TVSVPSDDTDDSN--FDCNICLDSVQEPVVTLCGHLFCWPCIHKWLDVQSFSTSDEYQRH 92

Query: 90  QNCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGMVVPRRPPP---SMNTPPHSNATS 149
           + CPVCK+ ++ S+LVPLYGRG   +  E K S     VP+RP      +  P    A++
Sbjct: 93  RQCPVCKSKVSHSTLVPLYGRGRCTTQEEGKNS-----VPKRPVGPVYRLEMPNSPYAST 152

Query: 150 ALHPSQELHPNYVRSPSHPIYHQQYFPQAYGDFASYAPSYLGNAVITSLLNPTIGMFGET 209
            L  SQ +H N   SP      + Y+P + G  +S + SY      +++L+P + M GE 
Sbjct: 153 DLRLSQRVHFN---SP-----QEGYYPVS-GVMSSNSLSY------SAVLDPVMVMVGEM 212

Query: 210 VFTRIFGSVDGNLLPYPPYNNAIPGNASTRMRRQEMQLDKSLNRVSIFLFCCFIICLLLF 265
           V TR+FG+   +   YP   N + G +  RMRR+ MQ DKSL R+  F  CC ++CLLLF
Sbjct: 213 VATRLFGTRVMDRFAYPDTYN-LAGTSGPRMRRRIMQADKSLGRIFFFFMCCVVLCLLLF 249

[Close]

BLAST of Bhi09G002793 vs. ExPASy Swiss-Prot
Match: P93030 (E3 ubiquitin-protein ligase RMA2 OS=Arabidopsis thaliana OX=3702 GN=RMA2 PE=1 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 5.0e-28
Identity = 79/236 (33.47%), Postives = 109/236 (46.19%), Query Frame = 0


Query: 36  TVSEDANGCFDCNICLDSAAEPVVTLCGHLYCWPCIYKWLHVQISS-------NRPENTQ 95
           T   D+ G FDCNICLD   +PVVTLCGHL+CWPCI+KW +   +S       +      
Sbjct: 10  TTLVDSGGDFDCNICLDQVRDPVVTLCGHLFCWPCIHKWTYASNNSRQRVDQYDHKREPP 69

Query: 96  NCPVCKASITPSSLVPLYGRGTSNSDSESKKSHLGMVVPRRPPPSMNTPPHSNATSALHP 155
            CPVCK+ ++ ++LVP+YGRG        K    G  VP R                   
Sbjct: 70  KCPVCKSDVSEATLVPIYGRG-------QKAPQSGSNVPSR------------------- 129

Query: 156 SQELHPNYVRSPSHPIYHQQYFPQAYGDFASYAPSYLGNAVITSLLNPTIGMFGETVFTR 215
                      P+ P+Y  +   Q  G+  S    Y        + +P +G+  E V+ R
Sbjct: 130 -----------PTGPVYDLRGVGQRLGEGESQRYMY-------RMPDPVMGVVCEMVYRR 189

Query: 216 IFGSVDGNLLPYPPYNNAIPGNASTRMRRQEMQLDKSLNRVSIFLFCCFIICLLLF 265
           +FG    N+ PY   N         R RR+ MQ ++SL+RV +FL C   +CL LF
Sbjct: 190 LFGESSSNMAPYRDMN--------VRSRRRAMQAEESLSRVYLFLLCFMFMCLFLF 193

[Close]

BLAST of Bhi09G002793 vs. ExPASy Swiss-Prot
Match: Q09463 (E3 ubiquitin ligase rnf-5 OS=Caenorhabditis elegans OX=6239 GN=rnf-5 PE=1 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 4.3e-19
Identity = 48/104 (46.15%), Postives = 66/104 (63.46%), Query Frame = 0


Query: 35  STVSEDANGCFDCNICLDSAAEPVVTLCGHLYCWPCIYKWLHVQISSNRPENTQNCPVCK 94
           S+ ++D +  F+CNICLD+A + VV+LCGHL+CWPC+ +WL       RP N Q CPVCK
Sbjct: 14  SSSNKDESARFECNICLDAAKDAVVSLCGHLFCWPCLSQWL-----DTRP-NNQVCPVCK 73

Query: 95  ASITPSSLVPLYGRGTSNSDSESKKSHLGMVVPRRPPPSMNTPP 139
           ++I  + +VP+YGRG  +SD   K       VP RP    + PP
Sbjct: 74  SAIDGNKVVPIYGRGGDSSDPRKK-------VPPRPKGQRSEPP 104

[Close]

BLAST of Bhi09G002793 vs. ExPASy TrEMBL
Match: A0A5A7VD79 (E3 ubiquitin-protein ligase RMA3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003900 PE=4 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 4.3e-139
Identity = 245/264 (92.80%), Postives = 254/264 (96.21%), Query Frame = 0


Query: 1   MEQNYSVPHSVSEAYLESEQDVSLKQNWKSISTQSTVSEDANGCFDCNICLDSAAEPVVT 60
           MEQNYSVP    EAYLESEQDVSLKQNWKSIST+ST+SEDANGCFDCNICLDSAA+PVVT
Sbjct: 1   MEQNYSVP----EAYLESEQDVSLKQNWKSISTESTISEDANGCFDCNICLDSAADPVVT 60

Query: 61  LCGHLYCWPCIYKWLHVQISSNRPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKS 120
           LCGHLYCWPCIYKWLHVQISSN PENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKS
Sbjct: 61  LCGHLYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKS 120

Query: 121 HLGMVVPRRPPPSMNTPPHSNATSALHPSQELHPNYVRSPSHPIYHQQYFPQAYGDFASY 180
           HLGM VPRRPPPSMNTPP SN++SA +PSQELH NY+RSPSHPIYHQQYFPQAYG+FASY
Sbjct: 121 HLGMAVPRRPPPSMNTPPPSNSSSASYPSQELHSNYIRSPSHPIYHQQYFPQAYGNFASY 180

Query: 181 APSYLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNNAIPGNASTRMRRQEM 240
           APSYLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNN+I GNASTRMRRQEM
Sbjct: 181 APSYLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNNSISGNASTRMRRQEM 240

Query: 241 QLDKSLNRVSIFLFCCFIICLLLF 265
           QLDKSLNRVSIFLFCCFIICLLLF
Sbjct: 241 QLDKSLNRVSIFLFCCFIICLLLF 260

[Close]

BLAST of Bhi09G002793 vs. ExPASy TrEMBL
Match: A0A1S3BTX0 (E3 ubiquitin-protein ligase RMA3 OS=Cucumis melo OX=3656 GN=LOC103493490 PE=4 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 4.3e-139
Identity = 245/264 (92.80%), Postives = 254/264 (96.21%), Query Frame = 0


Query: 1   MEQNYSVPHSVSEAYLESEQDVSLKQNWKSISTQSTVSEDANGCFDCNICLDSAAEPVVT 60
           MEQNYSVP    EAYLESEQDVSLKQNWKSIST+ST+SEDANGCFDCNICLDSAA+PVVT
Sbjct: 1   MEQNYSVP----EAYLESEQDVSLKQNWKSISTESTISEDANGCFDCNICLDSAADPVVT 60

Query: 61  LCGHLYCWPCIYKWLHVQISSNRPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKS 120
           LCGHLYCWPCIYKWLHVQISSN PENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKS
Sbjct: 61  LCGHLYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKS 120

Query: 121 HLGMVVPRRPPPSMNTPPHSNATSALHPSQELHPNYVRSPSHPIYHQQYFPQAYGDFASY 180
           HLGM VPRRPPPSMNTPP SN++SA +PSQELH NY+RSPSHPIYHQQYFPQAYG+FASY
Sbjct: 121 HLGMAVPRRPPPSMNTPPPSNSSSASYPSQELHSNYIRSPSHPIYHQQYFPQAYGNFASY 180

Query: 181 APSYLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNNAIPGNASTRMRRQEM 240
           APSYLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNN+I GNASTRMRRQEM
Sbjct: 181 APSYLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNNSISGNASTRMRRQEM 240

Query: 241 QLDKSLNRVSIFLFCCFIICLLLF 265
           QLDKSLNRVSIFLFCCFIICLLLF
Sbjct: 241 QLDKSLNRVSIFLFCCFIICLLLF 260

[Close]

BLAST of Bhi09G002793 vs. ExPASy TrEMBL
Match: A0A0A0KZN0 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G637750 PE=4 SV=1)

HSP 1 Score: 497.7 bits (1280), Expect = 3.1e-137
Identity = 244/265 (92.08%), Postives = 253/265 (95.47%), Query Frame = 0


Query: 1   MEQNYSVPHSVSEAYLESEQDVSLKQNWKSISTQSTVSEDANGCFDCNICLDSAAEPVVT 60
           MEQNYSVP    EAYLESEQDVSLKQNWKSISTQST+SEDANGCFDCNICLDSAA+PVVT
Sbjct: 1   MEQNYSVP----EAYLESEQDVSLKQNWKSISTQSTISEDANGCFDCNICLDSAADPVVT 60

Query: 61  LCGHLYCWPCIYKWLHVQISSNRPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKS 120
           LCGHLYCWPCIYKWLHVQISSN PENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKS
Sbjct: 61  LCGHLYCWPCIYKWLHVQISSNEPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKS 120

Query: 121 HLGMVVPRRPPPSMNTPPHSNATSALHPSQELHPNYVRSPSHPIYHQQYFPQA-YGDFAS 180
           HLGM VPRRPPPSMNTP HSN++S L+PSQELH NY+RSPSHPIYHQQYFPQA YG+FAS
Sbjct: 121 HLGMAVPRRPPPSMNTPSHSNSSSTLYPSQELHSNYIRSPSHPIYHQQYFPQATYGNFAS 180

Query: 181 YAPSYLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNNAIPGNASTRMRRQE 240
           Y+PSYLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPY PYNN+I GNASTRMRRQE
Sbjct: 181 YSPSYLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYSPYNNSISGNASTRMRRQE 240

Query: 241 MQLDKSLNRVSIFLFCCFIICLLLF 265
           MQLDKSLNRVSIFLFCCFIICLLLF
Sbjct: 241 MQLDKSLNRVSIFLFCCFIICLLLF 261

[Close]

BLAST of Bhi09G002793 vs. ExPASy TrEMBL
Match: A0A6J1CDN1 (E3 ubiquitin-protein ligase RMA3-like OS=Momordica charantia OX=3673 GN=LOC111010508 PE=4 SV=1)

HSP 1 Score: 473.4 bits (1217), Expect = 6.2e-130
Identity = 232/265 (87.55%), Postives = 244/265 (92.08%), Query Frame = 0


Query: 1   MEQNYSVPHSVSEAYLESEQDVSLKQNWKSISTQSTVSEDANGCFDCNICLDSAAEPVVT 60
           MEQ Y+ P    EAY ESEQDVSLKQNWKSIS+Q TVS+DA+GCFDCNICLDSAAEPVVT
Sbjct: 1   MEQTYTTP----EAYFESEQDVSLKQNWKSISSQPTVSDDAHGCFDCNICLDSAAEPVVT 60

Query: 61  LCGHLYCWPCIYKWLHVQISSNRPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKS 120
           LCGHLYCWPCIYKWL+VQISSN PEN QNCPVCKASITPSSLVPLYGRG SN+DSESKKS
Sbjct: 61  LCGHLYCWPCIYKWLNVQISSNEPENVQNCPVCKASITPSSLVPLYGRGKSNTDSESKKS 120

Query: 121 HLGMVVPRRPPPS-MNTPPHSNATSALHPSQELHPNYVRSPSHPIYHQQYFPQAYGDFAS 180
           HLG+ VPRRPPPS  NT  +SNATSALHPSQ+LHPNY RSPSHPIYHQQYFPQAYGD A+
Sbjct: 121 HLGVAVPRRPPPSATNTLHNSNATSALHPSQDLHPNYTRSPSHPIYHQQYFPQAYGDIAT 180

Query: 181 YAPSYLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNNAIPGNASTRMRRQE 240
           Y PSYLGNAVITSLLNPTIGMFGETVFTRIFGSVD NLLPYPPYNN+IPGN S+RMRRQE
Sbjct: 181 YTPSYLGNAVITSLLNPTIGMFGETVFTRIFGSVDSNLLPYPPYNNSIPGNGSSRMRRQE 240

Query: 241 MQLDKSLNRVSIFLFCCFIICLLLF 265
           MQLDKSLNRVSIFLFCCFIICLLLF
Sbjct: 241 MQLDKSLNRVSIFLFCCFIICLLLF 261

[Close]

BLAST of Bhi09G002793 vs. ExPASy TrEMBL
Match: A0A6J1J1I0 (E3 ubiquitin-protein ligase RMA3-like OS=Cucurbita maxima OX=3661 GN=LOC111480472 PE=4 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 3.4e-128
Identity = 224/264 (84.85%), Postives = 240/264 (90.91%), Query Frame = 0


Query: 1   MEQNYSVPHSVSEAYLESEQDVSLKQNWKSISTQSTVSEDANGCFDCNICLDSAAEPVVT 60
           M+Q Y    S SEAYLESE DVSLKQNWKS+S QSTVSEDANGCFDCNICLDSAA+PVVT
Sbjct: 1   MDQTY----SASEAYLESEHDVSLKQNWKSMSAQSTVSEDANGCFDCNICLDSAADPVVT 60

Query: 61  LCGHLYCWPCIYKWLHVQISSNRPENTQNCPVCKASITPSSLVPLYGRGTSNSDSESKKS 120
           LCGHL+CWPCIYKW HVQISSN  ENT NCPVCKASIT SSLVPLYGRG SNSDSESKKS
Sbjct: 61  LCGHLFCWPCIYKWFHVQISSNETENTHNCPVCKASITLSSLVPLYGRGMSNSDSESKKS 120

Query: 121 HLGMVVPRRPPPSMNTPPHSNATSALHPSQELHPNYVRSPSHPIYHQQYFPQAYGDFASY 180
           HLG+ +PRRPPPSMNTPPHSNA SALHPS+ELHPNY+RSP H IYHQQYFPQAYG+FA Y
Sbjct: 121 HLGITIPRRPPPSMNTPPHSNAASALHPSEELHPNYIRSPLHRIYHQQYFPQAYGNFAPY 180

Query: 181 APSYLGNAVITSLLNPTIGMFGETVFTRIFGSVDGNLLPYPPYNNAIPGNASTRMRRQEM 240
           APSYLGNA++TSLLNPTIG+ GETV+TRIFGS DGNLLPYPPYN+++ GN  TRMRRQEM
Sbjct: 181 APSYLGNAMVTSLLNPTIGLIGETVYTRIFGSADGNLLPYPPYNHSVAGNIGTRMRRQEM 240

Query: 241 QLDKSLNRVSIFLFCCFIICLLLF 265
           QLDKSLNRVSIFLFCCFIICLLLF
Sbjct: 241 QLDKSLNRVSIFLFCCFIICLLLF 260

The following BLAST results are available for this feature:

BLAST of Bhi09G002793 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G27470.1	2.4e-49	45.90	RING membrane-anchor 3	[more]
AT4G03510.1	2.6e-40	42.92	RING membrane-anchor 1	[more]
AT4G03510.2	2.6e-40	42.92	RING membrane-anchor 1	[more]
AT4G28270.1	3.6e-29	33.47	RING membrane-anchor 2	[more]
AT1G74990.1	3.1e-25	52.48	RING/U-box superfamily protein	[more]

BLAST of Bhi09G002793 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GUK7	3.3e-48	45.90	E3 ubiquitin-protein ligase RMA3 OS=Arabidopsis thaliana OX=3702 GN=RMA3 PE=1 SV...	[more]
Q6R567	1.9e-43	43.98	E3 ubiquitin-protein ligase RMA1H1 OS=Capsicum annuum OX=4072 GN=RMA1H1 PE=1 SV=...	[more]
O64425	3.7e-39	42.92	E3 ubiquitin-protein ligase RMA1 OS=Arabidopsis thaliana OX=3702 GN=RMA1 PE=1 SV...	[more]
P93030	5.0e-28	33.47	E3 ubiquitin-protein ligase RMA2 OS=Arabidopsis thaliana OX=3702 GN=RMA2 PE=1 SV...	[more]
Q09463	4.3e-19	46.15	E3 ubiquitin ligase rnf-5 OS=Caenorhabditis elegans OX=6239 GN=rnf-5 PE=1 SV=1	[more]

BLAST of Bhi09G002793 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7VD79	4.3e-139	92.80	E3 ubiquitin-protein ligase RMA3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3BTX0	4.3e-139	92.80	E3 ubiquitin-protein ligase RMA3 OS=Cucumis melo OX=3656 GN=LOC103493490 PE=4 SV...	[more]
A0A0A0KZN0	3.1e-137	92.08	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G637750 P...	[more]
A0A6J1CDN1	6.2e-130	87.55	E3 ubiquitin-protein ligase RMA3-like OS=Momordica charantia OX=3673 GN=LOC11101...	[more]
A0A6J1J1I0	3.4e-128	84.85	E3 ubiquitin-protein ligase RMA3-like OS=Cucurbita maxima OX=3661 GN=LOC11148047...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 47..93 e-value: 6.9E-10 score: 48.9
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 47..94 score: 12.84335
IPR018957	Zinc finger, C3HC4 RING-type	PFAM	PF00097	zf-C3HC4	coord: 47..93 e-value: 1.4E-8 score: 34.5
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 2..112 e-value: 9.6E-18 score: 65.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 106..148
None	No IPR available	PANTHER	PTHR12313:SF69	E3 UBIQUITIN-PROTEIN LIGASE RMA3-LIKE	coord: 12..264
None	No IPR available	CDD	cd16745	RING-HC_AtRMA_like	coord: 45..95 e-value: 4.42421E-25 score: 91.9819
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 27..100
IPR045103	E3 ubiquitin-protein ligase RNF5/RNF185-like	PANTHER	PTHR12313	E3 UBIQUITIN-PROTEIN LIGASE RNF5-RELATED	coord: 12..264
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 62..71

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi09M002793	Bhi09M002793	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071712	ER-associated misfolded protein catabolic process
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0044390	ubiquitin-like protein conjugating enzyme binding
molecular_function	GO:0061630	ubiquitin protein ligase activity