Bhi09G002733 (gene) Wax gourd (B227) v1

Overview


Name	Bhi09G002733
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Alpha-amylase
Location	chr9: 85227806 .. 85230456 (+)
RNA-Seq Expression	Bhi09G002733
Synteny	Bhi09G002733

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTCACAAGACAATAGAGCCTATTTTGCCCAATATCAGTTTCCCAATTGTTTGTAACTAGCTTCAGTTTAGTTTAGTTTAGTTTTTTTTTTATTTTTTATTATTATTATTTTTTATTTTTTATAATAACCTAAAACAATCCCTTGTTCTCCTGGCGCCTCTCTGCCTGTTGAAGGGCTACAAATACAGAGGACAATGGCAGATGGGATGGAGCTGAGGATTGAAAGAACTCCAATGGAAATTAAGCATTCTGGAGGATGGAGGGGTTTTCAATGTTGTTCATTTTACATTTTCATATTATGGCTATATTCAATATACTCAAACTTAAAACCACCATCGTTACTCTAGGTTTAGACATAGAGTATATACGGACATCAACAATATCATTTTTGTAAAGTATTTATAACGTCCCAGAATATGAATGAGAGGAATACTGGACTCGATTAGATAATTTGGTGGAATGCATGATCCAGTAGATGGATAATTTTTCTAAAATTCCCAAATAGGCTTCTACTGTGTGAGTGATCCTTACAAGCACGAAAAAAGATATTGCGTAGAAATCAACTCTTGTTTCTCGAATTAAAGGCTATATTGTCTTACGAACCACATTCAAACAACGAGTCTTACGTGTCACACACGATTAATTCTTTAAGTAGAAAACTTCTTACTTCTTGACTAGACCTTCTGCCTGCCCAGCATTCATTCATCAACCTCTTTCCTTTCTCACTCGTTCTCACTATCTACGCATCAAAAGAAACAAAGGCCTACGAAGTCATTCTCAAGACGCAAATCGAAGCTTATAAAACCAAAATTCCCACTGACCGATTCCCAGCAATGGGGTTTCTAGCTTCTCTGTGTTTCCTCTACCTCTGTGTTTCCCTCTTCCCATCCCCTTCTTCTTCTTCTACCTTACTGTTTCAGGTAAGCAAATCCACTTTACTACTTTCACTCTTCTCAATGGAAACTCACTTTTAATAGCTTGAAATTGATTGTAATGAAACATACAGGGCTTCAACTGGGAATCAAGCAGCAAAGGAGGATGGTACAACTCACTAAACAACATAATTCCTGATCTTCTAAGTGCTGAAGTTACCCATGTCTGGCTTCCTCCACCCTCCCAATCAGTTTCTTCACAAGGCAAGCTTTCTTTCTCTCTGTTCCTTCAGCTCATTACCAAGAACGTTTGTTTAAATTTGTGTAAATCCTCTTACAGGATACTTACCCGGAAGGCTTTATGATCTCAATGCATCGAAATATGGAACCCAGAATGAACTCAAGTCTCTAATTGCTACCCTCCATGAGAAGGGAATCAAAGCCATAGCTGACATAGTGATCAACCACAGAACAGCAGAGAAACAAGACCAGAGAGGAATTTGGTGTATTTTTGAAGGGGGAACTCCAGATAGCCGCCTTGATTGGGGCCCTTCCTTCATTTGCAGCGATGACACTGAATATTCCGATGGAAAGGGCAATCCAGACAGTGGGGAACCCTATCCACCAGCACCAGATATCGACCATCTCAATCCCCAAGTTCAGAAAGAATTATCAGAGTGGATGAGATGGTTGAAAACAGAAATAGGGTTTGATGGATGGAGATTCGATTTCGTAAAAGGCTACGCACCAAGTATTACAAAACTCTACATGGATCAAACCTCACCTGAATTTGCAGTCGGTGAGAAATGGGATGGACTTTCATATGGACAAGACGGGAAACCCAATCAAAACCAAGACTCACATCGCGATGCTCTTGCAAATTGGGTTAGGGCTTCGGGCGACATAGTCACAGCGTTTGATTTCACAACCAAAGGGATTCTTCAAGCTGCCGTTGAAGGAGAGCTATGGAGGTTGAAGGATTCTAACGGGAACCCGCCAGGATTCATCGGAATCAAGCCGGAAAGTGCCGTGACTTTCATCGAAAACCACGACACTGGCTCGACGCAAAGAATGTGGCCGTTCCCTTCCGATAAAGTGATTCAGGGATACGCATATATTCTGACTCATCCCGGAACTCCTTCCATTGTAAATCATTCAACAAAACAGTTTAGATTATTGAGTTTCGAAACTTGATTACCTGTAATTCAAAAACTGTTGGTTTGTTGCAGTTCTACGACCATTTCGTTGATTGGGGACTGAAGGAGGAGATAATGAAATTGAGTGGAATCAGGGCAAGGAATGGGATTGGGAAAACAAGCACGGTGAATATTATGGCTTCTGAGGGGGATCTGTACGTTGCTGGTGTTGATGGGAAAATTATAATGAAGATCGGAGCCAACACCAGTCTTGGGAATTTGATTCCGTCGGATTATCAGCTTGCCACTTATGGAATGGACTATGCTGTTTGGGAGAAAAGGTGAATCCTTGAATGTCTGCCATTGATGGAATAGTTCCTCTCTGCAGGTCCATTTCCATTTTCAAGAAAGTTTCCAAATTATATAATATAAATTAATTAATGATGTCTCAAACATTAAATGGGAGGAACAGGTACTTGCAGTTTTTAGCCGCAGCATATCATGCATCTATTTTCCATTTTTTTTTTTTTTTTTTTTTTTTGGCAAGATAGTAAATAATAGTAAGAAATTAATTTTTTTAAAGAGAGTTTCCCATACGACAGTCTTTCATTAGATAAGTGGTTTGGTTACACTTCCAGCAAGG

mRNA sequence

CTTCACAAGACAATAGAGCCTATTTTGCCCAATATCAGTTTCCCAATTGTTTGTAACTAGCTTCAGTTTAGTTTAGTTTAGTTTTTTTTTTATTTTTTATTATTATTATTTTTTATTTTTTATAATAACCTAAAACAATCCCTTGTTCTCCTGGCGCCTCTCTGCCTGTTGAAGGGCTACAAATACAGAGGACAATGGCAGATGGGATGGAGCTGAGGATTGAAAGAACTCCAATGGAAATTAAGCATTCTGGAGGATGGAGGGGTTTTCAATGTTGTTCATTTTACATTTTCATATTATGGCTATATTCAATATACTCAAACTTAAAACCACCATCGTTACTCTAGGTTTAGACATAGAGTATATACGGACATCAACAATATCATTTTTGTAAAGTATTTATAACGTCCCAGAATATGAATGAGAGGAATACTGGACTCGATTAGATAATTTGGTGGAATGCATGATCCAGTAGATGGATAATTTTTCTAAAATTCCCAAATAGGCTTCTACTGTGTGAGTGATCCTTACAAGCACGAAAAAAGATATTGCGTAGAAATCAACTCTTGTTTCTCGAATTAAAGGCTATATTGTCTTACGAACCACATTCAAACAACGAGTCTTACGTGTCACACACGATTAATTCTTTAAGTAGAAAACTTCTTACTTCTTGACTAGACCTTCTGCCTGCCCAGCATTCATTCATCAACCTCTTTCCTTTCTCACTCGTTCTCACTATCTACGCATCAAAAGAAACAAAGGCCTACGAAGTCATTCTCAAGACGCAAATCGAAGCTTATAAAACCAAAATTCCCACTGACCGATTCCCAGCAATGGGGTTTCTAGCTTCTCTGTGTTTCCTCTACCTCTGTGTTTCCCTCTTCCCATCCCCTTCTTCTTCTTCTACCTTACTGTTTCAGGGCTTCAACTGGGAATCAAGCAGCAAAGGAGGATGGTACAACTCACTAAACAACATAATTCCTGATCTTCTAAGTGCTGAAGTTACCCATGTCTGGCTTCCTCCACCCTCCCAATCAGTTTCTTCACAAGGATACTTACCCGGAAGGCTTTATGATCTCAATGCATCGAAATATGGAACCCAGAATGAACTCAAGTCTCTAATTGCTACCCTCCATGAGAAGGGAATCAAAGCCATAGCTGACATAGTGATCAACCACAGAACAGCAGAGAAACAAGACCAGAGAGGAATTTGGTGTATTTTTGAAGGGGGAACTCCAGATAGCCGCCTTGATTGGGGCCCTTCCTTCATTTGCAGCGATGACACTGAATATTCCGATGGAAAGGGCAATCCAGACAGTGGGGAACCCTATCCACCAGCACCAGATATCGACCATCTCAATCCCCAAGTTCAGAAAGAATTATCAGAGTGGATGAGATGGTTGAAAACAGAAATAGGGTTTGATGGATGGAGATTCGATTTCGTAAAAGGCTACGCACCAAGTATTACAAAACTCTACATGGATCAAACCTCACCTGAATTTGCAGTCGGTGAGAAATGGGATGGACTTTCATATGGACAAGACGGGAAACCCAATCAAAACCAAGACTCACATCGCGATGCTCTTGCAAATTGGGTTAGGGCTTCGGGCGACATAGTCACAGCGTTTGATTTCACAACCAAAGGGATTCTTCAAGCTGCCGTTGAAGGAGAGCTATGGAGGTTGAAGGATTCTAACGGGAACCCGCCAGGATTCATCGGAATCAAGCCGGAAAGTGCCGTGACTTTCATCGAAAACCACGACACTGGCTCGACGCAAAGAATGTGGCCGTTCCCTTCCGATAAAGTGATTCAGGGATACGCATATATTCTGACTCATCCCGGAACTCCTTCCATTTTCTACGACCATTTCGTTGATTGGGGACTGAAGGAGGAGATAATGAAATTGAGTGGAATCAGGGCAAGGAATGGGATTGGGAAAACAAGCACGGTGAATATTATGGCTTCTGAGGGGGATCTGTACGTTGCTGGTGTTGATGGGAAAATTATAATGAAGATCGGAGCCAACACCAGTCTTGGGAATTTGATTCCGTCGGATTATCAGCTTGCCACTTATGGAATGGACTATGCTGTTTGGGAGAAAAGGTGAATCCTTGAATGTCTGCCATTGATGGAATAGTTCCTCTCTGCAGGTCCATTTCCATTTTCAAGAAAGTTTCCAAATTATATAATATAAATTAATTAATGATGTCTCAAACATTAAATGGGAGGAACAGGTACTTGCAGTTTTTAGCCGCAGCATATCATGCATCTATTTTCCATTTTTTTTTTTTTTTTTTTTTTTTGGCAAGATAGTAAATAATAGTAAGAAATTAATTTTTTTAAAGAGAGTTTCCCATACGACAGTCTTTCATTAGATAAGTGGTTTGGTTACACTTCCAGCAAGG

Coding sequence (CDS)

ATGGGGTTTCTAGCTTCTCTGTGTTTCCTCTACCTCTGTGTTTCCCTCTTCCCATCCCCTTCTTCTTCTTCTACCTTACTGTTTCAGGGCTTCAACTGGGAATCAAGCAGCAAAGGAGGATGGTACAACTCACTAAACAACATAATTCCTGATCTTCTAAGTGCTGAAGTTACCCATGTCTGGCTTCCTCCACCCTCCCAATCAGTTTCTTCACAAGGATACTTACCCGGAAGGCTTTATGATCTCAATGCATCGAAATATGGAACCCAGAATGAACTCAAGTCTCTAATTGCTACCCTCCATGAGAAGGGAATCAAAGCCATAGCTGACATAGTGATCAACCACAGAACAGCAGAGAAACAAGACCAGAGAGGAATTTGGTGTATTTTTGAAGGGGGAACTCCAGATAGCCGCCTTGATTGGGGCCCTTCCTTCATTTGCAGCGATGACACTGAATATTCCGATGGAAAGGGCAATCCAGACAGTGGGGAACCCTATCCACCAGCACCAGATATCGACCATCTCAATCCCCAAGTTCAGAAAGAATTATCAGAGTGGATGAGATGGTTGAAAACAGAAATAGGGTTTGATGGATGGAGATTCGATTTCGTAAAAGGCTACGCACCAAGTATTACAAAACTCTACATGGATCAAACCTCACCTGAATTTGCAGTCGGTGAGAAATGGGATGGACTTTCATATGGACAAGACGGGAAACCCAATCAAAACCAAGACTCACATCGCGATGCTCTTGCAAATTGGGTTAGGGCTTCGGGCGACATAGTCACAGCGTTTGATTTCACAACCAAAGGGATTCTTCAAGCTGCCGTTGAAGGAGAGCTATGGAGGTTGAAGGATTCTAACGGGAACCCGCCAGGATTCATCGGAATCAAGCCGGAAAGTGCCGTGACTTTCATCGAAAACCACGACACTGGCTCGACGCAAAGAATGTGGCCGTTCCCTTCCGATAAAGTGATTCAGGGATACGCATATATTCTGACTCATCCCGGAACTCCTTCCATTTTCTACGACCATTTCGTTGATTGGGGACTGAAGGAGGAGATAATGAAATTGAGTGGAATCAGGGCAAGGAATGGGATTGGGAAAACAAGCACGGTGAATATTATGGCTTCTGAGGGGGATCTGTACGTTGCTGGTGTTGATGGGAAAATTATAATGAAGATCGGAGCCAACACCAGTCTTGGGAATTTGATTCCGTCGGATTATCAGCTTGCCACTTATGGAATGGACTATGCTGTTTGGGAGAAAAGGTGA

Protein sequence

MGFLASLCFLYLCVSLFPSPSSSSTLLFQGFNWESSSKGGWYNSLNNIIPDLLSAEVTHVWLPPPSQSVSSQGYLPGRLYDLNASKYGTQNELKSLIATLHEKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKELSEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDQTSPEFAVGEKWDGLSYGQDGKPNQNQDSHRDALANWVRASGDIVTAFDFTTKGILQAAVEGELWRLKDSNGNPPGFIGIKPESAVTFIENHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLSGIRARNGIGKTSTVNIMASEGDLYVAGVDGKIIMKIGANTSLGNLIPSDYQLATYGMDYAVWEKR

Homology

[Close]

BLAST of Bhi09G002733 vs. TAIR 10
Match: AT4G25000.1 (alpha-amylase-like )

HSP 1 Score: 569.3 bits (1466), Expect = 2.6e-162
Identity = 266/411 (64.72%), Postives = 328/411 (79.81%), Query Frame = 0


Query: 16  LFPSPSSSSTLLFQGFNWESSSK-GGWYNSLNNIIPDLLSAEVTHVWLPPPSQSVSSQGY 75
           +FP+ + SSTLLFQ FNWES  K GG+YNSL+N I D+ +A +TH+WLPPPSQSV+ +GY
Sbjct: 17  VFPTFTFSSTLLFQSFNWESWKKEGGFYNSLHNSIDDIANAGITHLWLPPPSQSVAPEGY 76

Query: 76  LPGRLYDLNASKYGTQNELKSLIATLHEKGIKAIADIVINHRTAEKQDQRGIWCIFEGGT 135
           LPG+LYDLN+SKYG++ ELKSLI  L++KGIKA+ADIVINHRTAE++D +  +C FEGGT
Sbjct: 77  LPGKLYDLNSSKYGSEAELKSLIKALNQKGIKALADIVINHRTAERKDDKCGYCYFEGGT 136

Query: 136 PDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKELSEWMRWLKTEI 195
            D RLDW PSF+C +D ++  G GN D+G  +  APDIDHLNP+VQKELSEWM WLKTEI
Sbjct: 137 SDDRLDWDPSFVCRNDPKF-PGTGNLDTGGDFDGAPDIDHLNPRVQKELSEWMNWLKTEI 196

Query: 196 GFDGWRFDFVKGYAPSITKLYMDQTSPEFAVGEKWDGLSYGQDGKPNQNQDSHRDALANW 255
           GF GWRFD+V+GYA SITKLY+  TSP+FAVGEKWD + YG DGK + +Q+ HR  L  W
Sbjct: 197 GFHGWRFDYVRGYASSITKLYVQNTSPDFAVGEKWDDMKYGGDGKLDYDQNEHRSGLKQW 256

Query: 256 V-RASGDIVTAFDFTTKGILQAAVEGELWRLKDSNGNPPGFIGIKPESAVTFIENHDTGS 315
           +  A G ++TAFDFTTKGILQ+AV+GELWRLKDS G PPG IGI P +AVTFI+NHD   
Sbjct: 257 IEEAGGGVLTAFDFTTKGILQSAVKGELWRLKDSQGKPPGMIGIMPGNAVTFIDNHD--- 316

Query: 316 TQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLSGIRARNGIGKTSTV 375
           T R W FPSDKV+ GY YILTHPGTP IFY+H+++WGLKE I KL  IR +NGIG TS+V
Sbjct: 317 TFRTWVFPSDKVLLGYVYILTHPGTPCIFYNHYIEWGLKESISKLVAIRNKNGIGSTSSV 376

Query: 376 NIMASEGDLYVAGVDGKIIMKIGANTSLGNLIPSDYQLATYGMDYAVWEKR 425
            I A+E DLY+A +D K+IMKIG    +G L+PS++ LA  G+D+AVWEK+
Sbjct: 377 TIKAAEADLYLAMIDDKVIMKIGPKQDVGTLVPSNFALAYSGLDFAVWEKK 423

[Close]

BLAST of Bhi09G002733 vs. TAIR 10
Match: AT1G69830.1 (alpha-amylase-like 3 )

HSP 1 Score: 377.1 bits (967), Expect = 1.9e-104
Identity = 190/411 (46.23%), Postives = 252/411 (61.31%), Query Frame = 0


Query: 14  VSLFPSPSSSSTLLFQGFNWESSSKGGWYNSLNNIIPDLLSAEVTHVWLPPPSQSVSSQG 73
           + +     S   +L QGFNWES+  G WY  L     +L S   T +WLPPP++SVS +G
Sbjct: 485 IKISSGTGSGFEILCQGFNWESNKSGRWYLELQEKADELASLGFTVLWLPPPTESVSPEG 544

Query: 74  YLPGRLYDLNASKYGTQNELKSLIATLHEKGIKAIADIVINHRTAEKQDQRGIWCIFEGG 133
           Y+P  LY+LN S+YGT +ELK  +   H+ GIK + D V+NHR A  ++Q G+W +F G 
Sbjct: 545 YMPKDLYNLN-SRYGTIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGG- 604

Query: 134 TPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKELSEWMRWLKTE 193
               RL+W    + +DD  +  G+GN  SG+ +  AP+IDH    V+K++ EW+ W+  E
Sbjct: 605 ----RLNWDDRAVVADDPHF-QGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWMMEE 664

Query: 194 IGFDGWRFDFVKGYAPSITKLYMDQTSPEFAVGEKWDGLSYGQDGKPNQNQDSHRDALAN 253
           +G+DGWR DFV+G+     K YMD + P FAVGE WD LSY   G+ + NQD+HR  + +
Sbjct: 665 VGYDGWRLDFVRGFWGGYVKDYMDASKPYFAVGEYWDSLSYTY-GEMDYNQDAHRQRIVD 724

Query: 254 WVRASGDIVTAFDFTTKGILQAAVEG-ELWRLKDSNGNPPGFIGIKPESAVTFIENHDTG 313
           W+ A+     AFD TTKGIL  A++  E WRL D  G PPG +G  P  AVTFIENHDTG
Sbjct: 725 WINATSGAAGAFDVTTKGILHTALQKCEYWRLSDPKGKPPGVVGWWPSRAVTFIENHDTG 784

Query: 314 STQRMWPFPSDKVIQGYAYILTHPGTPSIFYDH-FVDWGLKEEIMKLSGIRARNGIGKTS 373
           STQ  W FP  K +QGYAYILTHPGTP++F+DH F D+    EI  L  +R R  +   S
Sbjct: 785 STQGHWRFPEGKEMQGYAYILTHPGTPAVFFDHIFSDY--HSEIAALLSLRNRQKLHCRS 844

Query: 374 TVNIMASEGDLYVAGVDGKIIMKIGANTSLGNLIPSDYQLATYGMDYAVWE 423
            VNI  SE D+Y A +D K+ MKIG           ++ +A  G DY VWE
Sbjct: 845 EVNIDKSERDVYAAIIDEKVAMKIGPGHYEPPNGSQNWSVAVEGRDYKVWE 885

[Close]

BLAST of Bhi09G002733 vs. TAIR 10
Match: AT1G76130.1 (alpha-amylase-like 2 )

HSP 1 Score: 368.6 bits (945), Expect = 6.6e-102
Identity = 184/402 (45.77%), Postives = 255/402 (63.43%), Query Frame = 0


Query: 26  LLFQGFNWESSSKGGWYNSLNNIIPDLLSAEVTHVWLPPPSQSVSSQGYLPGRLYDLNAS 85
           ++ Q +NWE S K  W+ +L+  +PD+  +  T  WLPPPSQS++ +GYLP  LY LN S
Sbjct: 27  VILQAYNWE-SHKYDWWRNLDGKVPDIAKSGFTSAWLPPPSQSLAPEGYLPQDLYSLN-S 86

Query: 86  KYGTQNELKSLIATLHEKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTPDSRLDWGPSF 145
            YG+++ LKSL+  + +  ++A+ADIVINHR    +   G++  ++G      L W    
Sbjct: 87  AYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTRGHGGMYNRYDG----ISLPWDEHA 146

Query: 146 ICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKELSEWMRWLKTEIGFDGWRFDFVK 205
           +    T  + G GN  +G+ +   P++DH    V+K++  W+RWL+  +GF  +RFDF +
Sbjct: 147 V----TSCTGGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRWLRNTVGFQDFRFDFAR 206

Query: 206 GYAPSITKLYMDQTSPEFAVGEKWDGLSYGQDGKPNQNQDSHRDALANWVRASGDIVTAF 265
           GY+ +  K Y+    P F+VGE WD  +Y   G  + NQDSHR  + +W+ A+G I  AF
Sbjct: 207 GYSANYVKEYIGAAKPLFSVGECWDSCNYNGHGL-DYNQDSHRQRIISWIDATGQISAAF 266

Query: 266 DFTTKGILQAAVEGELWRLKDSNGNPPGFIGIKPESAVTFIENHDTGSTQRMWPFPSDKV 325
           DFTTKGILQ AV+G+ WRL D+ G PPG +G  P  AVTF++NHDTGSTQ  WPFPS  V
Sbjct: 267 DFTTKGILQEAVKGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPSHHV 326

Query: 326 IQGYAYILTHPGTPSIFYDHFVDWG--LKEEIMKLSGIRARNGIGKTSTVNIMASEGDLY 385
           ++GYAYILTHPG P +FYDHF DWG  + ++I+KL  IR R  I   STV ++ +E +LY
Sbjct: 327 MEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVLKAESNLY 386

Query: 386 VAGVDGKIIMKIGANTSLGNLIPS--DYQLATYGMDYAVWEK 424
            A V  KI MK+G     G+  PS  D+ LAT G  YAVW K
Sbjct: 387 AAIVGEKICMKLGD----GSWCPSGRDWTLATSGHRYAVWHK 413

[Close]

BLAST of Bhi09G002733 vs. ExPASy Swiss-Prot
Match: P17859 (Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1)

HSP 1 Score: 694.5 bits (1791), Expect = 7.4e-199
Identity = 325/422 (77.01%), Postives = 359/422 (85.07%), Query Frame = 0


Query: 1   MGFLASLCFLYLCVSLFPSPSSSSTLLFQGFNWESSSKGGWYNSLNNIIPDLLSAEVTHV 60
           M   + L    L +SL P   SS  LLFQGFNWESS KGGWYNSL N IPDL +A +THV
Sbjct: 1   MDSFSRLSIFCLFISLLPL-FSSPALLFQGFNWESSKKGGWYNSLKNSIPDLANAGITHV 60

Query: 61  WLPPPSQSVSSQGYLPGRLYDLNASKYGTQNELKSLIATLHEKGIKAIADIVINHRTAEK 120
           WLPPPSQSVS +GYLPGRLYDL+ASKYG++NELKSLIA  HEKGIK +ADIVINHRTAE+
Sbjct: 61  WLPPPSQSVSPEGYLPGRLYDLDASKYGSKNELKSLIAAFHEKGIKCLADIVINHRTAER 120

Query: 121 QDQRGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQ 180
           +D RGI+CIFEGGTPDSR DWGPSFIC DDT YSDG GN DSGE Y  APDIDHLNPQVQ
Sbjct: 121 KDGRGIYCIFEGGTPDSRQDWGPSFICRDDTAYSDGTGNNDSGEGYDAAPDIDHLNPQVQ 180

Query: 181 KELSEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDQTSPEFAVGEKWDGLSYGQDGKP 240
           +ELSEWM WLKTEIGFDGWRFDFVKGYAPSI+K+YM+QT P+FAVGEKWD +SYGQDGKP
Sbjct: 181 RELSEWMNWLKTEIGFDGWRFDFVKGYAPSISKIYMEQTKPDFAVGEKWDSISYGQDGKP 240

Query: 241 NQNQDSHRDALANWVRASGDIVTAFDFTTKGILQAAVEGELWRLKDSNGNPPGFIGIKPE 300
           N NQDSHR AL NWV ++G  +TAFDFTTKGILQAAV+GELWRL D NG PPG IG+KPE
Sbjct: 241 NYNQDSHRGALVNWVESAGGAITAFDFTTKGILQAAVQGELWRLIDPNGKPPGMIGVKPE 300

Query: 301 SAVTFIENHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLSG 360
           +AVTFI+NHDTGSTQR+WPFPSDKV+QGYAYILTHPGTPSIFYDHF DWGLKE+I KLS 
Sbjct: 301 NAVTFIDNHDTGSTQRLWPFPSDKVMQGYAYILTHPGTPSIFYDHFFDWGLKEQIAKLSS 360

Query: 361 IRARNGIGKTSTVNIMASEGDLYVAGVDGKIIMKIGANTSLGNLIPSDYQLATYGMDYAV 420
           IR RNGI + STV IMASEGDLYVA +D KI++KIG    LGNLIPS+  +AT G DYAV
Sbjct: 361 IRLRNGINEKSTVKIMASEGDLYVAKIDNKIMVKIGPKMDLGNLIPSNLHVATSGQDYAV 420

Query: 421 WE 423
           WE
Sbjct: 421 WE 421

[Close]

BLAST of Bhi09G002733 vs. ExPASy Swiss-Prot
Match: P04063 (Alpha-amylase type B isozyme OS=Hordeum vulgare OX=4513 GN=AMY1.2 PE=1 SV=3)

HSP 1 Score: 570.1 bits (1468), Expect = 2.1e-161
Identity = 261/417 (62.59%), Postives = 316/417 (75.78%), Query Frame = 0


Query: 10  LYLCVSLFPSPSSSSTLLFQGFNWES-SSKGGWYNSLNNIIPDLLSAEVTHVWLPPPSQS 69
           L+L +    +  +S  +LFQGFNWES    GGWYN L   + D+ +A +THVWLPP SQS
Sbjct: 10  LFLVLLGLSASLASGQVLFQGFNWESWKHNGGWYNFLMGKVDDIAAAGITHVWLPPASQS 69

Query: 70  VSSQGYLPGRLYDLNASKYGTQNELKSLIATLHEKGIKAIADIVINHRTAEKQDQRGIWC 129
           V+ QGY+PGRLYDL+ASKYG + +LKSLI  LH KG+KAIADIVINHRTAE +D RGI+C
Sbjct: 70  VAEQGYMPGRLYDLDASKYGNKAQLKSLIGALHGKGVKAIADIVINHRTAEHKDGRGIYC 129

Query: 130 IFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKELSEWMR 189
           IFEGGTPD+RLDWGP  IC DD  Y+DG GNPD+G  +  APDIDHLN +VQKEL EW+ 
Sbjct: 130 IFEGGTPDARLDWGPHMICRDDRPYADGTGNPDTGADFGAAPDIDHLNLRVQKELVEWLN 189

Query: 190 WLKTEIGFDGWRFDFVKGYAPSITKLYMDQTSPEFAVGEKWDGLSYGQDGKPNQNQDSHR 249
           WLK +IGFDGWRFDF KGY+  + K+Y+D++ P FAV E W  L+YG DGKPN NQD HR
Sbjct: 190 WLKADIGFDGWRFDFAKGYSADVAKIYIDRSEPSFAVAEIWTSLAYGGDGKPNLNQDQHR 249

Query: 250 DALANWVRASG--DIVTAFDFTTKGILQAAVEGELWRLKDSNGNPPGFIGIKPESAVTFI 309
             L NWV   G     T FDFTTKGIL  AVEGELWRL+ ++G  PG IG  P  AVTF+
Sbjct: 250 QELVNWVDKVGGKGPATTFDFTTKGILNVAVEGELWRLRGTDGKAPGMIGWWPAKAVTFV 309

Query: 310 ENHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLSGIRARNG 369
           +NHDTGSTQ MWPFPSD+V+QGYAYILTHPGTP IFYDHF DWGLKEEI +L  +R R+G
Sbjct: 310 DNHDTGSTQHMWPFPSDRVMQGYAYILTHPGTPCIFYDHFFDWGLKEEIDRLVSVRTRHG 369

Query: 370 IGKTSTVNIMASEGDLYVAGVDGKIIMKIGANTSLGNLIPSDYQLATYGMDYAVWEK 424
           I   S + I+ ++ DLY+A +DGK+I+K+G    +GNLIP  +++A +G DYAVWEK
Sbjct: 370 IHNESKLQIIEADADLYLAEIDGKVIVKLGPRYDVGNLIPGGFKVAAHGNDYAVWEK 426

[Close]

BLAST of Bhi09G002733 vs. ExPASy Swiss-Prot
Match: Q8VZ56 (Alpha-amylase 1 OS=Arabidopsis thaliana OX=3702 GN=AMY1 PE=1 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 3.6e-161
Identity = 266/411 (64.72%), Postives = 328/411 (79.81%), Query Frame = 0


Query: 16  LFPSPSSSSTLLFQGFNWESSSK-GGWYNSLNNIIPDLLSAEVTHVWLPPPSQSVSSQGY 75
           +FP+ + SSTLLFQ FNWES  K GG+YNSL+N I D+ +A +TH+WLPPPSQSV+ +GY
Sbjct: 17  VFPTFTFSSTLLFQSFNWESWKKEGGFYNSLHNSIDDIANAGITHLWLPPPSQSVAPEGY 76

Query: 76  LPGRLYDLNASKYGTQNELKSLIATLHEKGIKAIADIVINHRTAEKQDQRGIWCIFEGGT 135
           LPG+LYDLN+SKYG++ ELKSLI  L++KGIKA+ADIVINHRTAE++D +  +C FEGGT
Sbjct: 77  LPGKLYDLNSSKYGSEAELKSLIKALNQKGIKALADIVINHRTAERKDDKCGYCYFEGGT 136

Query: 136 PDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKELSEWMRWLKTEI 195
            D RLDW PSF+C +D ++  G GN D+G  +  APDIDHLNP+VQKELSEWM WLKTEI
Sbjct: 137 SDDRLDWDPSFVCRNDPKF-PGTGNLDTGGDFDGAPDIDHLNPRVQKELSEWMNWLKTEI 196

Query: 196 GFDGWRFDFVKGYAPSITKLYMDQTSPEFAVGEKWDGLSYGQDGKPNQNQDSHRDALANW 255
           GF GWRFD+V+GYA SITKLY+  TSP+FAVGEKWD + YG DGK + +Q+ HR  L  W
Sbjct: 197 GFHGWRFDYVRGYASSITKLYVQNTSPDFAVGEKWDDMKYGGDGKLDYDQNEHRSGLKQW 256

Query: 256 V-RASGDIVTAFDFTTKGILQAAVEGELWRLKDSNGNPPGFIGIKPESAVTFIENHDTGS 315
           +  A G ++TAFDFTTKGILQ+AV+GELWRLKDS G PPG IGI P +AVTFI+NHD   
Sbjct: 257 IEEAGGGVLTAFDFTTKGILQSAVKGELWRLKDSQGKPPGMIGIMPGNAVTFIDNHD--- 316

Query: 316 TQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLSGIRARNGIGKTSTV 375
           T R W FPSDKV+ GY YILTHPGTP IFY+H+++WGLKE I KL  IR +NGIG TS+V
Sbjct: 317 TFRTWVFPSDKVLLGYVYILTHPGTPCIFYNHYIEWGLKESISKLVAIRNKNGIGSTSSV 376

Query: 376 NIMASEGDLYVAGVDGKIIMKIGANTSLGNLIPSDYQLATYGMDYAVWEKR 425
            I A+E DLY+A +D K+IMKIG    +G L+PS++ LA  G+D+AVWEK+
Sbjct: 377 TIKAAEADLYLAMIDDKVIMKIGPKQDVGTLVPSNFALAYSGLDFAVWEKK 423

[Close]

BLAST of Bhi09G002733 vs. ExPASy Swiss-Prot
Match: P27934 (Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2 SV=1)

HSP 1 Score: 565.8 bits (1457), Expect = 4.0e-160
Identity = 251/419 (59.90%), Postives = 316/419 (75.42%), Query Frame = 0


Query: 6   SLCFLYLCVSLFPSPSSSSTLLFQGFNWESSSK-GGWYNSLNNIIPDLLSAEVTHVWLPP 65
           +LC +   V    S  + + +LFQGFNWES  K GGWYN L+  + ++ S   THVWLPP
Sbjct: 7   TLCCVVFAVLCLASSLAQAQVLFQGFNWESWRKQGGWYNFLHEKVEEIASTGATHVWLPP 66

Query: 66  PSQSVSSQGYLPGRLYDLNASKYGTQNELKSLIATLHEKGIKAIADIVINHRTAEKQDQR 125
           PS SVS QGY+PGRLYDL+ASKYGT+ ELKSLI   H+K ++ +ADIVINHR A+ +D R
Sbjct: 67  PSHSVSPQGYMPGRLYDLDASKYGTEAELKSLIEAFHDKNVECLADIVINHRCADYKDSR 126

Query: 126 GIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKELS 185
           G++C+FEGGTPD RLDWGP  ICSDDT+YS+G+G+ D+G  +  APDIDHLNP+VQ+EL+
Sbjct: 127 GVYCVFEGGTPDGRLDWGPDMICSDDTQYSNGRGHRDTGAGFGAAPDIDHLNPRVQRELT 186

Query: 186 EWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDQTSPEFAVGEKWDGLSYGQDGKPNQNQ 245
           +W+ WL+T++GFDGWR DF KGY+  + ++Y+D T+P F VGE W  L Y  DGKP+ NQ
Sbjct: 187 DWLNWLRTDLGFDGWRLDFAKGYSAPLARIYVDNTNPTFVVGEIWSSLIYNGDGKPSTNQ 246

Query: 246 DSHRDALANWVRASGDIVTAFDFTTKGILQAAVEGELWRLKDSNGNPPGFIGIKPESAVT 305
           D+ R  L NWV   G   TAFDFTTKGILQAAV+GELWRL D NG  PG +G  P+ AVT
Sbjct: 247 DADRQELVNWVEGVGKPATAFDFTTKGILQAAVQGELWRLHDGNGKAPGLMGWMPDQAVT 306

Query: 306 FIENHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLSGIRAR 365
           F++NHDTGSTQ +WPFPSDKV+QGYAYILTHPG P IFYDH  DW L+ EI  L+ IR+R
Sbjct: 307 FVDNHDTGSTQSLWPFPSDKVMQGYAYILTHPGIPCIFYDHVFDWNLQHEIATLAEIRSR 366

Query: 366 NGIGKTSTVNIMASEGDLYVAGVDGKIIMKIGANTSLGNLIPSDYQLATYGMDYAVWEK 424
           NGI   ST++I+ +EGD+YVA +DGK+I K+G     G +IPSD+ +  +G DY VWEK
Sbjct: 367 NGIHAESTLDILKAEGDIYVAMIDGKVITKLGPRYDAGGIIPSDFHVVAHGNDYCVWEK 425

[Close]

BLAST of Bhi09G002733 vs. ExPASy Swiss-Prot
Match: P27939 (Alpha-amylase isozyme 3C OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.7 PE=2 SV=2)

HSP 1 Score: 563.9 bits (1452), Expect = 1.5e-159
Identity = 254/417 (60.91%), Postives = 314/417 (75.30%), Query Frame = 0


Query: 8   CFLYLCVSLFPSPSSSSTLLFQGFNWESSSK-GGWYNSLNNIIPDLLSAEVTHVWLPPPS 67
           C L+  +    S  + + +LFQGFNWES  K GGWYN L++ + D+ +  VTHVWLPPPS
Sbjct: 10  CLLFFVLLCLGSHLAQAQVLFQGFNWESWKKQGGWYNFLHSHVDDIAATGVTHVWLPPPS 69

Query: 68  QSVSSQGYLPGRLYDLNASKYGTQNELKSLIATLHEKGIKAIADIVINHRTAEKQDQRGI 127
            SV+ QGY+PGRLYDL+ASKYGT  EL+SLIA  H K IK +ADIVINHR A+ +D RGI
Sbjct: 70  HSVAPQGYMPGRLYDLDASKYGTGAELRSLIAAFHSKSIKCVADIVINHRCADYKDSRGI 129

Query: 128 WCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQKELSEW 187
           +CIFEGGTPDSRLDWGP  ICSDDT+YS+G+G+ D+G  +  APDIDHLN +VQ ELS+W
Sbjct: 130 YCIFEGGTPDSRLDWGPDMICSDDTQYSNGRGHRDTGADFGAAPDIDHLNTRVQTELSDW 189

Query: 188 MRWLKTEIGFDGWRFDFVKGYAPSITKLYMDQTSPEFAVGEKWDGLSYGQDGKPNQNQDS 247
           + WLK+++GFDGWR DF KGY+ ++ K Y+D T P F V E W  + Y  +G+P+ NQD 
Sbjct: 190 LNWLKSDVGFDGWRLDFAKGYSATVAKTYVDNTDPSFVVAEIWSNMRYDGNGEPSWNQDG 249

Query: 248 HRDALANWVRASGDIVTAFDFTTKGILQAAVEGELWRLKDSNGNPPGFIGIKPESAVTFI 307
            R  L NW +A G   +AFDFTTKG LQAAV+GELWR+KD NG  PG IG  PE AVTFI
Sbjct: 250 DRQELVNWAQAVGGPASAFDFTTKGELQAAVQGELWRMKDGNGKAPGMIGWLPEKAVTFI 309

Query: 308 ENHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLSGIRARNG 367
           +NHDTGSTQ  WPFPSDKV+QGYAYILTHPG P IFYDH  DW LK+EI  L+ +R+RNG
Sbjct: 310 DNHDTGSTQNSWPFPSDKVMQGYAYILTHPGVPCIFYDHVFDWNLKQEISTLAAVRSRNG 369

Query: 368 IGKTSTVNIMASEGDLYVAGVDGKIIMKIGANTSLGNLIPSDYQLATYGMDYAVWEK 424
           I   S +NI+A++GD+YVA +D K+I KIG    +GNLIPSD+ +  +G +Y VWEK
Sbjct: 370 IHPGSKLNILAADGDVYVAMIDDKVITKIGTRYDVGNLIPSDFHVVAHGNNYCVWEK 426

[Close]

BLAST of Bhi09G002733 vs. ExPASy TrEMBL
Match: A0A6J1CMA4 (Alpha-amylase OS=Momordica charantia OX=3673 GN=LOC111012587 PE=3 SV=1)

HSP 1 Score: 809.3 bits (2089), Expect = 7.6e-231
Identity = 378/424 (89.15%), Postives = 406/424 (95.75%), Query Frame = 0


Query: 1   MGFLASLCFLYLCVSLFPSPSSSSTLLFQGFNWESSSKGGWYNSLNNIIPDLLSAEVTHV 60
           MGFLASLCFLYL VSLFPSPSSS+ LLFQGFNWESSSKGGWYNSL NIIPDL++A +THV
Sbjct: 16  MGFLASLCFLYLSVSLFPSPSSSA-LLFQGFNWESSSKGGWYNSLKNIIPDLVNARITHV 75

Query: 61  WLPPPSQSVSSQGYLPGRLYDLNASKYGTQNELKSLIATLHEKGIKAIADIVINHRTAEK 120
           WLPPPSQSVS QGYLPGRLYDL+ASKYGTQNELKSLIA LHEKGIKAIADIVINHRTAEK
Sbjct: 76  WLPPPSQSVSPQGYLPGRLYDLDASKYGTQNELKSLIAALHEKGIKAIADIVINHRTAEK 135

Query: 121 QDQRGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQ 180
           QDQRGIWCIFEGGTPD+RLDWGPSFICSDDT+YSDGKGNPDSGEPYPPAPDIDHL PQVQ
Sbjct: 136 QDQRGIWCIFEGGTPDNRLDWGPSFICSDDTDYSDGKGNPDSGEPYPPAPDIDHLKPQVQ 195

Query: 181 KELSEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDQTSPEFAVGEKWDGLSYGQDGKP 240
           KE+SEWMRWLKTEIGFDGWRFDFVKGYAPS+TKLYMDQTSP+FAVGEKWD LS G DGK 
Sbjct: 196 KEISEWMRWLKTEIGFDGWRFDFVKGYAPSVTKLYMDQTSPDFAVGEKWDSLSDGPDGKL 255

Query: 241 NQNQDSHRDALANWVRASGDIVTAFDFTTKGILQAAVEGELWRLKDSNGNPPGFIGIKPE 300
           NQNQD+HRD+LANWV+ASGD VTAFDFTTKGILQAAV+GELWRLKDSNG PPGFIGIKPE
Sbjct: 256 NQNQDAHRDSLANWVKASGDAVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKPE 315

Query: 301 SAVTFIENHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLSG 360
           SAVTF++NHDTGSTQ++WPFPSDKVIQGYAYILTHPGTP+IFYDHFV+WGLKEEI++LS 
Sbjct: 316 SAVTFVDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPAIFYDHFVEWGLKEEIIRLSE 375

Query: 361 IRARNGIGKTSTVNIMASEGDLYVAGVDGKIIMKIGANTSLGNLIPSDYQLATYGMDYAV 420
           IRARN IGKTS VNI+AS+ DLYVAGVDGKIIMKIGANT+LGNLIPS++QLAT GM+YAV
Sbjct: 376 IRARNRIGKTSAVNILASDADLYVAGVDGKIIMKIGANTNLGNLIPSEFQLATSGMEYAV 435

Query: 421 WEKR 425
           WEKR
Sbjct: 436 WEKR 438

[Close]

BLAST of Bhi09G002733 vs. ExPASy TrEMBL
Match: A0A6J1FCS4 (Alpha-amylase OS=Cucurbita moschata OX=3662 GN=LOC111442931 PE=3 SV=1)

HSP 1 Score: 800.8 bits (2067), Expect = 2.7e-228
Identity = 375/425 (88.24%), Postives = 397/425 (93.41%), Query Frame = 0


Query: 1   MGFLASLCFL-YLCVSLFPSPSSSSTLLFQGFNWESSSKGGWYNSLNNIIPDLLSAEVTH 60
           MGFLASLCFL YL V LFPSPS SSTLLFQGFNWESSSKGGWYNSLNNIIPDL+ A+VTH
Sbjct: 25  MGFLASLCFLCYLSVFLFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH 84

Query: 61  VWLPPPSQSVSSQGYLPGRLYDLNASKYGTQNELKSLIATLHEKGIKAIADIVINHRTAE 120
           VWLPPPSQS S QGYLPGRLYDLNASKYGTQNELKSLIA LH  GIKA+ADIVINHRTA+
Sbjct: 85  VWLPPPSQSASPQGYLPGRLYDLNASKYGTQNELKSLIAALHNNGIKAVADIVINHRTAD 144

Query: 121 KQDQRGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQV 180
           KQD+RGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPY  APDIDHLNPQV
Sbjct: 145 KQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYSAAPDIDHLNPQV 204

Query: 181 QKELSEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDQTSPEFAVGEKWDGLSYGQDGK 240
           Q ELSEWMRWLKTEIGFDGWRFDFVKGYAP ITKLYMDQTSP+FAVGEKWD LS G DGK
Sbjct: 205 QNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDGK 264

Query: 241 PNQNQDSHRDALANWVRASGDIVTAFDFTTKGILQAAVEGELWRLKDSNGNPPGFIGIKP 300
           P+ NQD HR+ALANWVR SGD VTAFDFTTKGILQAAV+GELWRLKDSNG PPGFIGIKP
Sbjct: 265 PDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKP 324

Query: 301 ESAVTFIENHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLS 360
           ESAVTFI+NHDTGSTQ++WPFPSDKVIQGYAYILTHPGTPSIFYDHFV+WGLKEE++KLS
Sbjct: 325 ESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLS 384

Query: 361 GIRARNGIGKTSTVNIMASEGDLYVAGVDGKIIMKIGANTSLGNLIPSDYQLATYGMDYA 420
            IRARNGIG+ STVNIMAS+GDLYVAG+DGKIIMKIG NT+LGNL+PSDY+LA  GM YA
Sbjct: 385 AIRARNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLVPSDYELAASGMQYA 444

Query: 421 VWEKR 425
           VWEK+
Sbjct: 445 VWEKK 449

[Close]

BLAST of Bhi09G002733 vs. ExPASy TrEMBL
Match: A0A6J1IGI6 (Alpha-amylase OS=Cucurbita maxima OX=3661 GN=LOC111476734 PE=3 SV=1)

HSP 1 Score: 796.6 bits (2056), Expect = 5.1e-227
Identity = 373/425 (87.76%), Postives = 396/425 (93.18%), Query Frame = 0


Query: 1   MGFLASLCFL-YLCVSLFPSPSSSSTLLFQGFNWESSSKGGWYNSLNNIIPDLLSAEVTH 60
           MGFLASLCFL YL V +FPSPS SSTLLFQGFNWESSSKGGWYNSLNNIIPDL+ A+VTH
Sbjct: 1   MGFLASLCFLCYLSVFIFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH 60

Query: 61  VWLPPPSQSVSSQGYLPGRLYDLNASKYGTQNELKSLIATLHEKGIKAIADIVINHRTAE 120
           VWLPPPSQS S QGYLPGRLYDLNASKYGTQ+ELKSLIA LH  GIKA+ADIVINHRTA+
Sbjct: 61  VWLPPPSQSASPQGYLPGRLYDLNASKYGTQDELKSLIAALHNNGIKAVADIVINHRTAD 120

Query: 121 KQDQRGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQV 180
           KQD+RGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPY  APDIDHLNPQV
Sbjct: 121 KQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYSAAPDIDHLNPQV 180

Query: 181 QKELSEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDQTSPEFAVGEKWDGLSYGQDGK 240
           Q ELSEWMRWLKTEIGFDGWRFDFVKGYAP ITKLYMDQTSP+FAVGEKWD LS G DGK
Sbjct: 181 QNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSPGPDGK 240

Query: 241 PNQNQDSHRDALANWVRASGDIVTAFDFTTKGILQAAVEGELWRLKDSNGNPPGFIGIKP 300
           P+ NQD HR+ALANWVR SGD VTAFDFTTKGILQAAV+GELWRLKD NG PPGFIGIKP
Sbjct: 241 PDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQGELWRLKDPNGKPPGFIGIKP 300

Query: 301 ESAVTFIENHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLS 360
           ESAVTFI+NHDTGSTQ++WPFPSDKVIQGYAYILTHPGTPSIFYDHFV+WGLKEE++KLS
Sbjct: 301 ESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLS 360

Query: 361 GIRARNGIGKTSTVNIMASEGDLYVAGVDGKIIMKIGANTSLGNLIPSDYQLATYGMDYA 420
            IR RNGIG+ STVNIMAS+GDLYVAG+DGKIIMKIG NT+LGNLIPSDY+LAT GM YA
Sbjct: 361 AIRTRNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLIPSDYELATSGMQYA 420

Query: 421 VWEKR 425
           VWEK+
Sbjct: 421 VWEKK 425

[Close]

BLAST of Bhi09G002733 vs. ExPASy TrEMBL
Match: A0A2N9FNN7 (Alpha-amylase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS20239 PE=3 SV=1)

HSP 1 Score: 704.5 bits (1817), Expect = 2.7e-199
Identity = 325/424 (76.65%), Postives = 366/424 (86.32%), Query Frame = 0


Query: 1   MGFLASLCFLYLCVSLFPSPSSSSTLLFQGFNWESSSKGGWYNSLNNIIPDLLSAEVTHV 60
           M  L SLCFL LC+SL PS  +   +LFQGFNWESS+KGGWYN L N IPDL +A +THV
Sbjct: 1   MNILTSLCFLCLCISLSPS-FAVPAILFQGFNWESSNKGGWYNLLKNSIPDLANAGITHV 60

Query: 61  WLPPPSQSVSSQGYLPGRLYDLNASKYGTQNELKSLIATLHEKGIKAIADIVINHRTAEK 120
           WLPPPSQSV+ QGY+PGRLYDL+ASKYG+++ELKSLIA LHEKGIKA+ADIVINHRTAEK
Sbjct: 61  WLPPPSQSVAPQGYMPGRLYDLDASKYGSKDELKSLIAALHEKGIKALADIVINHRTAEK 120

Query: 121 QDQRGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQ 180
           QD RGIWCIFEGGTPD RLDWGPSFIC DDT YSDG GNPDSG+ +  APDIDH+NPQVQ
Sbjct: 121 QDGRGIWCIFEGGTPDGRLDWGPSFICKDDTAYSDGTGNPDSGKDFGGAPDIDHVNPQVQ 180

Query: 181 KELSEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDQTSPEFAVGEKWDGLSYGQDGKP 240
           KELSEWM WLKTEIGFDGWRFDFV GYA S TK+YM+QT P+FAVGE W+ LSYG  GKP
Sbjct: 181 KELSEWMNWLKTEIGFDGWRFDFVLGYASSFTKVYMEQTKPDFAVGENWNSLSYGDGGKP 240

Query: 241 NQNQDSHRDALANWVRASGDIVTAFDFTTKGILQAAVEGELWRLKDSNGNPPGFIGIKPE 300
           N NQD+HR+AL +W++A+G  VTAFDFTTKGILQAAVEGELWRLKDSNG PPG IGIKPE
Sbjct: 241 NPNQDAHRNALVDWIQAAGGAVTAFDFTTKGILQAAVEGELWRLKDSNGKPPGLIGIKPE 300

Query: 301 SAVTFIENHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLSG 360
           +AVTFI+NHDTGSTQ++WPFPSDKVIQGYAYILTHPGTPSIFYDH  DWGLK+EI KL+ 
Sbjct: 301 NAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHLFDWGLKDEISKLTA 360

Query: 361 IRARNGIGKTSTVNIMASEGDLYVAGVDGKIIMKIGANTSLGNLIPSDYQLATYGMDYAV 420
           IR+RNGI     VNI+AS+ DLYVAG+D KIIMKIG    LGNLIPS++Q++  G DYAV
Sbjct: 361 IRSRNGINTQGIVNILASDADLYVAGIDDKIIMKIGPKMDLGNLIPSNFQVSASGKDYAV 420

Query: 421 WEKR 425
           WEK+
Sbjct: 421 WEKK 423

[Close]

BLAST of Bhi09G002733 vs. ExPASy TrEMBL
Match: A0A2I4EXA7 (Alpha-amylase OS=Juglans regia OX=51240 GN=LOC108993530 PE=3 SV=1)

HSP 1 Score: 702.2 bits (1811), Expect = 1.3e-198
Identity = 326/424 (76.89%), Postives = 364/424 (85.85%), Query Frame = 0


Query: 1   MGFLASLCFLYLCVSLFPSPSSSSTLLFQGFNWESSSKGGWYNSLNNIIPDLLSAEVTHV 60
           M     LCFL L +SL  S ++ S LLFQGFNWESS KGGWYNSL N +PDL +AE+THV
Sbjct: 1   MNLKTFLCFLCLFISLCHSFAAPS-LLFQGFNWESSKKGGWYNSLKNFVPDLANAEITHV 60

Query: 61  WLPPPSQSVSSQGYLPGRLYDLNASKYGTQNELKSLIATLHEKGIKAIADIVINHRTAEK 120
           WLPPPSQS + +GYLPGRLYDL+ SKYG+++ELK+LIA LHEKGIK +ADIVINHRTAEK
Sbjct: 61  WLPPPSQSAAPEGYLPGRLYDLDVSKYGSKDELKALIAALHEKGIKVVADIVINHRTAEK 120

Query: 121 QDQRGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQ 180
           QD RGIW IFEGGTPD+RLDWGPSFIC DD  YSDG GNPDSGE Y PAPDIDH NPQVQ
Sbjct: 121 QDDRGIWSIFEGGTPDARLDWGPSFICKDDIAYSDGTGNPDSGEGYDPAPDIDHRNPQVQ 180

Query: 181 KELSEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDQTSPEFAVGEKWDGLSYGQDGKP 240
           KELSEWM WLKTEIG+DGWRFDFVKGYAPSI K YM+QT P+FAVGE W+ L+YG DGKP
Sbjct: 181 KELSEWMNWLKTEIGYDGWRFDFVKGYAPSIMKNYMEQTKPDFAVGENWNSLAYGDDGKP 240

Query: 241 NQNQDSHRDALANWVRASGDIVTAFDFTTKGILQAAVEGELWRLKDSNGNPPGFIGIKPE 300
           N NQD+HR ALA WV ++G  VTAFDFTTKGILQAAVEGELWRLKDSNG PPG IGIKPE
Sbjct: 241 NPNQDAHRGALAEWVGSAGGAVTAFDFTTKGILQAAVEGELWRLKDSNGKPPGLIGIKPE 300

Query: 301 SAVTFIENHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLSG 360
           SAVTFI+NHDTGSTQ +WPFPSDKV+QGYAYILTHPGTP+IFYDHF DWGLKEEI KL+ 
Sbjct: 301 SAVTFIDNHDTGSTQNIWPFPSDKVMQGYAYILTHPGTPTIFYDHFFDWGLKEEISKLTA 360

Query: 361 IRARNGIGKTSTVNIMASEGDLYVAGVDGKIIMKIGANTSLGNLIPSDYQLATYGMDYAV 420
           IR+RNGI   S V+I+AS+GDLYVAG+D KIIMKIG    LGNLIPS++Q++T G DYAV
Sbjct: 361 IRSRNGINTRSIVSILASDGDLYVAGIDDKIIMKIGPKMDLGNLIPSNFQVSTSGKDYAV 420

Query: 421 WEKR 425
           WEK+
Sbjct: 421 WEKK 423

The following BLAST results are available for this feature:

BLAST of Bhi09G002733 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25000.1	2.6e-162	64.72	alpha-amylase-like	[more]
AT1G69830.1	1.9e-104	46.23	alpha-amylase-like 3	[more]
AT1G76130.1	6.6e-102	45.77	alpha-amylase-like 2	[more]

BLAST of Bhi09G002733 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P17859	7.4e-199	77.01	Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1	[more]
P04063	2.1e-161	62.59	Alpha-amylase type B isozyme OS=Hordeum vulgare OX=4513 GN=AMY1.2 PE=1 SV=3	[more]
Q8VZ56	3.6e-161	64.72	Alpha-amylase 1 OS=Arabidopsis thaliana OX=3702 GN=AMY1 PE=1 SV=1	[more]
P27934	4.0e-160	59.90	Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2...	[more]
P27939	1.5e-159	60.91	Alpha-amylase isozyme 3C OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.7 PE=2...	[more]

BLAST of Bhi09G002733 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CMA4	7.6e-231	89.15	Alpha-amylase OS=Momordica charantia OX=3673 GN=LOC111012587 PE=3 SV=1	[more]
A0A6J1FCS4	2.7e-228	88.24	Alpha-amylase OS=Cucurbita moschata OX=3662 GN=LOC111442931 PE=3 SV=1	[more]
A0A6J1IGI6	5.1e-227	87.76	Alpha-amylase OS=Cucurbita maxima OX=3661 GN=LOC111476734 PE=3 SV=1	[more]
A0A2N9FNN7	2.7e-199	76.65	Alpha-amylase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS20239 PE=3 SV=1	[more]
A0A2I4EXA7	1.3e-198	76.89	Alpha-amylase OS=Juglans regia OX=51240 GN=LOC108993530 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006046	Alpha amylase	PRINTS	PR00110	ALPHAAMYLASE	coord: 300..312 score: 44.19 coord: 196..207 score: 40.85 coord: 104..115 score: 49.51
IPR006047	Glycosyl hydrolase, family 13, catalytic domain	SMART	SM00642	aamy	coord: 25..362 e-value: 1.8E-51 score: 186.9
IPR006047	Glycosyl hydrolase, family 13, catalytic domain	PFAM	PF00128	Alpha-amylase	coord: 57..208 e-value: 8.8E-14 score: 51.8
IPR012850	Alpha-amylase, C-terminal beta-sheet	SMART	SM00810	alpha-amyl_c2	coord: 363..423 e-value: 1.5E-29 score: 114.2
IPR012850	Alpha-amylase, C-terminal beta-sheet	PFAM	PF07821	Alpha-amyl_C2	coord: 364..422 e-value: 1.3E-16 score: 60.6
IPR013780	Glycosyl hydrolase, all-beta	GENE3D	2.60.40.1180		coord: 367..424 e-value: 7.6E-22 score: 79.2
IPR013775	Alpha-amylase, plant	PIRSF	PIRSF001028	Alpha-amylase_plant	coord: 1..424 e-value: 4.2E-216 score: 715.7
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 25..366 e-value: 8.7E-143 score: 477.9
None	No IPR available	PIRSR	PIRSR001021-2	PIRSR001021-2	coord: 170..369 e-value: 1.7E-13 score: 48.1
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 150..172
None	No IPR available	PANTHER	PTHR43447:SF37	ALPHA-AMYLASE 1	coord: 1..424
None	No IPR available	PANTHER	PTHR43447	ALPHA-AMYLASE	coord: 1..424
None	No IPR available	CDD	cd11314	AmyAc_arch_bac_plant_AmyA	coord: 29..373 e-value: 5.86564E-165 score: 463.616
None	No IPR available	SUPERFAMILY	51011	Glycosyl hydrolase domain	coord: 367..423
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 26..352

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi09M002733	Bhi09M002733	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004556	alpha-amylase activity
molecular_function	GO:0103025	alpha-amylase activity (releasing maltohexaose)
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0043169	cation binding