Bhi09G002726 (gene) Wax gourd (B227) v1

Overview


Name	Bhi09G002726
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Alpha/beta-Hydrolases superfamily protein, putative
Location	chr9: 85057551 .. 85058549 (+)
RNA-Seq Expression	Bhi09G002726
Synteny	Bhi09G002726

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ACTTCCAAAACCGAGCAACGACTCAAGCATTTATGTTTCAAACCACCTCAGATCCAAACATAATAGTGGTGGCTTTCAGAGGGACTTCCCCACTGGACGCCTTCGATTGGCAAGTCAATGTGGACTTCTCTTGGTATGATATTCATGGTGTGGGTCGTATTCACAGCGGTTTCATGAAAGCTCTCGGCCTCCAAAAGCGCAAAGGTTGGCCTAAAGAACTCATTCCAACAACCACCACTCCCCGCCAATTTGCCTACTACACCCTCCGTCAAAAACTCTTTGACATTGCCAAAACCAATGCCAATGCAAAGTTCATCTTAACCGGCCACAGCTTAGGGGCAGCTCTTGCTATCCTCTTCGTCGCCATACTTGCCCTTCACGACGAGTCATTGGTGCTAGAGAAGATACAGGCCATCTACAGCTATGGCCAGCCAAGAGCCGGTGACCGACATTTTGCAGAGTTTATGGTAAGCATCATCCAAAAATACAACTTCGAATATCACAGATACGTTTACTTTAGTGACTTGGTGCCTAGACTTCCTGCTGATGGCATCCTTTTCAAATACAAACACTTTGGAAGATGCATCTATTTTGACAGCTTTTACAGAGGCAGAGTAAGAACAAAATTAAACAACCCTCTCTCCTCACTTGTCAAAAAACGACTTATAATAGGAATAACATTGAATGTTTGTTTCTTCTTTGTGGGTACAGATTGTGAAGGAACAGCCAAACAAGAACTATTTCTCGTTGCTATGGCTGATGCCTAAGTATTTAAATGCATGGTTGGAGCTTGTAAGGAGTTTCGTAATCCCATTTGTGAAAGGTTATGATTACTATGAGAGTTTACTTATGAAGATGGTAAGGTTTATTGGACTGTTTATGCCAGGACTCACCGCTCACATTCCTACAGACTATGTTAATTCCACTCGTTTAGGAAAATTAAATGTATCTGAGGAGATTCTTGAAGATGGAGACGACTGTATAGAACCTGACTACTAA

mRNA sequence

ACTTCCAAAACCGAGCAACGACTCAAGCATTTATGTTTCAAACCACCTCAGATCCAAACATAATAGTGGTGGCTTTCAGAGGGACTTCCCCACTGGACGCCTTCGATTGGCAAGTCAATGTGGACTTCTCTTGGTATGATATTCATGGTGTGGGTCGTATTCACAGCGGTTTCATGAAAGCTCTCGGCCTCCAAAAGCGCAAAGGTTGGCCTAAAGAACTCATTCCAACAACCACCACTCCCCGCCAATTTGCCTACTACACCCTCCGTCAAAAACTCTTTGACATTGCCAAAACCAATGCCAATGCAAAGTTCATCTTAACCGGCCACAGCTTAGGGGCAGCTCTTGCTATCCTCTTCGTCGCCATACTTGCCCTTCACGACGAGTCATTGGTGCTAGAGAAGATACAGGCCATCTACAGCTATGGCCAGCCAAGAGCCGGTGACCGACATTTTGCAGAGTTTATGATTGTGAAGGAACAGCCAAACAAGAACTATTTCTCGTTGCTATGGCTGATGCCTAAGTATTTAAATGCATGGTTGGAGCTTGTAAGGAGTTTCGTAATCCCATTTGTGAAAGGTTATGATTACTATGAGAGTTTACTTATGAAGATGGTAAGGTTTATTGGACTGTTTATGCCAGGACTCACCGCTCACATTCCTACAGACTATGTTAATTCCACTCGTTTAGGAAAATTAAATGTATCTGAGGAGATTCTTGAAGATGGAGACGACTGTATAGAACCTGACTACTAA

Coding sequence (CDS)

ACTTCCAAAACCGAGCAACGACTCAAGCATTTATGTTTCAAACCACCTCAGATCCAAACATAATAGTGGTGGCTTTCAGAGGGACTTCCCCACTGGACGCCTTCGATTGGCAAGTCAATGTGGACTTCTCTTGGTATGATATTCATGGTGTGGGTCGTATTCACAGCGGTTTCATGAAAGCTCTCGGCCTCCAAAAGCGCAAAGGTTGGCCTAAAGAACTCATTCCAACAACCACCACTCCCCGCCAATTTGCCTACTACACCCTCCGTCAAAAACTCTTTGACATTGCCAAAACCAATGCCAATGCAAAGTTCATCTTAACCGGCCACAGCTTAGGGGCAGCTCTTGCTATCCTCTTCGTCGCCATACTTGCCCTTCACGACGAGTCATTGGTGCTAGAGAAGATACAGGCCATCTACAGCTATGGCCAGCCAAGAGCCGGTGACCGACATTTTGCAGAGTTTATGATTGTGAAGGAACAGCCAAACAAGAACTATTTCTCGTTGCTATGGCTGATGCCTAAGTATTTAAATGCATGGTTGGAGCTTGTAAGGAGTTTCGTAATCCCATTTGTGAAAGGTTATGATTACTATGAGAGTTTACTTATGAAGATGGTAAGGTTTATTGGACTGTTTATGCCAGGACTCACCGCTCACATTCCTACAGACTATGTTAATTCCACTCGTTTAGGAAAATTAAATGTATCTGAGGAGATTCTTGAAGATGGAGACGACTGTATAGAACCTGACTACTAA

Protein sequence

FQNRATTQAFMFQTTSDPNIIVVAFRGTSPLDAFDWQVNVDFSWYDIHGVGRIHSGFMKALGLQKRKGWPKELIPTTTTPRQFAYYTLRQKLFDIAKTNANAKFILTGHSLGAALAILFVAILALHDESLVLEKIQAIYSYGQPRAGDRHFAEFMIVKEQPNKNYFSLLWLMPKYLNAWLELVRSFVIPFVKGYDYYESLLMKMVRFIGLFMPGLTAHIPTDYVNSTRLGKLNVSEEILEDGDDCIEPDY

Homology

[Close]

BLAST of Bhi09G002726 vs. TAIR 10
Match: AT5G42930.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 238.8 bits (608), Expect = 4.7e-63
Identity = 121/284 (42.61%), Postives = 169/284 (59.51%), Query Frame = 0


Query: 1   FQNRATTQAFMFQTTS-DPNIIVVAFRGTSPLDAFDWQVNVDFSWYDIHGVGRIHSGFMK 60
           +Q + +T+  + + TS DPN+I+V+FRGT P DA DW  ++D SWY++  VG+IH GFMK
Sbjct: 179 YQKQRSTEVIVIKDTSTDPNLIIVSFRGTDPFDADDWCTDLDLSWYEVKNVGKIHGGFMK 238

Query: 61  ALGLQKRKGWPKE--LIPTTTTPRQFAYYTLRQKLFDIAKTNANAKFILTGHSLGAALAI 120
           ALGLQK +GWPKE  L  T      +AYYT+R+ L +I   N  +KFILTGHSLG ALAI
Sbjct: 239 ALGLQK-EGWPKEVNLDETQNATTLYAYYTVRRHLKEILDQNPTSKFILTGHSLGGALAI 298

Query: 121 LFVAILALHDESLVLEKIQAIYSYGQPRAGDRHFAEFM---------------------- 180
           LF A+L +HDE  +LE+++ +Y++GQPR GD  F  FM                      
Sbjct: 299 LFTAVLVMHDEEQMLERLEGVYTFGQPRVGDEEFGNFMKDSLKKFDVKYERYVYCNDMVP 358

Query: 181 ---------------------------IVKEQPNKNYFSLLWLMPKYLNAWLELVRSFVI 233
                                      + +E+PNKNYF+L+W++PK +NA  EL+RSFV+
Sbjct: 359 RLPFDDKTLMFKHFGACLYYDSFYKGKVEEEEPNKNYFNLVWVLPKIMNALWELIRSFVM 418

[Close]

BLAST of Bhi09G002726 vs. TAIR 10
Match: AT1G45201.1 (triacylglycerol lipase-like 1 )

HSP 1 Score: 233.8 bits (595), Expect = 1.5e-61
Identity = 126/283 (44.52%), Postives = 168/283 (59.36%), Query Frame = 0


Query: 1   FQNRATTQAFMFQTTS-DPNIIVVAFRGTSPLDAFDWQVNVDFSWYDIHGVGRIHSGFMK 60
           FQ    TQAF+F+T+S +P++IVV+FRGT P +A DW  ++D SWY++  VG++H+GF +
Sbjct: 184 FQESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWYEMKNVGKVHAGFSR 243

Query: 61  ALGLQKRKGWPKELIPTTTTPRQFAYYTLRQKLFDIAKTNANAKFILTGHSLGAALAILF 120
           ALGLQK  GWPKE I   +   Q+AYYT+RQ L D    N N K+ILTGHSLG ALA LF
Sbjct: 244 ALGLQK-DGWPKENI---SLLHQYAYYTIRQMLRDKLGRNKNLKYILTGHSLGGALAALF 303

Query: 121 VAILALHDESLVLEKIQAIYSYGQPRAGDRHFAEFM------------------------ 180
            AILA+H E  +L+K++ IY++GQPR GD  F EFM                        
Sbjct: 304 PAILAIHGEDELLDKLEGIYTFGQPRVGDEDFGEFMKGVVKKHGIEYERFVYNNDVVPRV 363

Query: 181 --------------------------IVKEQPNKNYFSLLWLMPKYLNAWLELVRSFVIP 233
                                     + ++ PN NYF+LLWL+P+ L    E +RSF++ 
Sbjct: 364 PFDDKYLFSYKHYGPCNSFNSLYKGKVREDAPNANYFNLLWLIPQLLTGLWEFIRSFILQ 423

[Close]

BLAST of Bhi09G002726 vs. TAIR 10
Match: AT1G56630.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 210.7 bits (535), Expect = 1.4e-54
Identity = 110/280 (39.29%), Postives = 160/280 (57.14%), Query Frame = 0


Query: 2   QNRATTQAFMFQTTSDPNIIVVAFRGTSPLDAFDWQVNVDFSWYDIHGVGRIHSGFMKAL 61
           Q R+T    +  T  +PN+IVV+FRGT P +A DW  ++D SW+++  VG+IH GFMKAL
Sbjct: 183 QTRSTEVIVIRDTKDNPNLIVVSFRGTDPFNADDWCTDLDLSWHNVMNVGKIHGGFMKAL 242

Query: 62  GLQKRKGWPKELIPTTTTPR--QFAYYTLRQKLFDIAKTNANAKFILTGHSLGAALAILF 121
           GL K +GW +E+    T  +  Q AYYT+ ++L ++ + N  +KFIL+GHSLG ALAILF
Sbjct: 243 GLPK-EGWYEEINSDQTQNKTSQLAYYTILRQLKEVFEQNPTSKFILSGHSLGGALAILF 302

Query: 122 VAILALHDESLVLEKIQAIYSYGQPRAGDRHFAEFM------------------------ 181
            A+L +HDE  +LE+++ +Y++GQPR GD  F  +M                        
Sbjct: 303 TAVLIMHDEKEMLERLEGVYTFGQPRVGDEDFGIYMKDKLKEFDVKYKRYVYCNDMVPRL 362

Query: 182 -------------------------IVKEQPNKNYFSLLWLMPKYLNAWLELVRSFVIPF 231
                                    + +E+PNKNYF++ W++PK +NA  EL+RSF+I  
Sbjct: 363 PFDDKTLMFKHFGGCLYCDSFYKGKVEEEEPNKNYFNIFWVIPKIINAVWELIRSFIISC 422

[Close]

BLAST of Bhi09G002726 vs. TAIR 10
Match: AT5G67050.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 197.2 bits (500), Expect = 1.6e-50
Identity = 109/285 (38.25%), Postives = 153/285 (53.68%), Query Frame = 0


Query: 1   FQNRATTQAFMF---QTTSDPN----IIVVAFRGTSPLDAFDWQVNVDFSWYDIHGVGRI 60
           +Q + TTQAF+    +TT+  N     +VVAFRGT   ++ DW  + D +W+++  +G I
Sbjct: 178 YQEKETTQAFIMSTDETTTRSNGQETTVVVAFRGTELFNSEDWCSDFDITWFELPNIGNI 237

Query: 61  HSGFMKALGLQKRKGWPKELIPTTTTPRQFAYYTLRQKLFDIAKTNANAKFILTGHSLGA 120
           H GFMKALGLQ    WPKE +         AYY++R  L  +   N N KF+LTGHSLG 
Sbjct: 238 HGGFMKALGLQNNCSWPKEPLSNPDRKSPLAYYSIRDSLKTLIAQNKNTKFVLTGHSLGG 297

Query: 121 ALAILFVAILALHDESLVLEKIQAIYSYGQPRAGDRHFAEFM------------------ 180
           ALAILF A+L +H E+ +LE+IQ +Y+YGQPR GD  F EFM                  
Sbjct: 298 ALAILFTAVLVIHHETELLERIQGVYTYGQPRVGDSKFGEFMEKKLEKYNIKYYRFVYNN 357

Query: 181 -------------------------------IVKEQPNKNYFSLLWLMPKYLNAWLELVR 230
                                          +++EQ ++N+F L  ++    +A LE +R
Sbjct: 358 DIVPRLPYDDKDLMFKHFGTCIYYDQNYQAKVMREQSDENFFLLRGIIKMMWSAILEFIR 417

[Close]

BLAST of Bhi09G002726 vs. TAIR 10
Match: AT1G45201.2 (triacylglycerol lipase-like 1 )

HSP 1 Score: 174.5 bits (441), Expect = 1.1e-43
Identity = 87/156 (55.77%), Postives = 114/156 (73.08%), Query Frame = 0


Query: 1   FQNRATTQAFMFQTTS-DPNIIVVAFRGTSPLDAFDWQVNVDFSWYDIHGVGRIHSGFMK 60
           FQ    TQAF+F+T+S +P++IVV+FRGT P +A DW  ++D SWY++  VG++H+GF +
Sbjct: 184 FQESKLTQAFVFKTSSTNPDLIVVSFRGTEPFEAADWCTDLDLSWYEMKNVGKVHAGFSR 243

Query: 61  ALGLQKRKGWPKELIPTTTTPRQFAYYTLRQKLFDIAKTNANAKFILTGHSLGAALAILF 120
           ALGLQK  GWPKE I   +   Q+AYYT+RQ L D    N N K+ILTGHSLG ALA LF
Sbjct: 244 ALGLQK-DGWPKENI---SLLHQYAYYTIRQMLRDKLGRNKNLKYILTGHSLGGALAALF 303

Query: 121 VAILALHDESLVLEKIQAIYSYGQPRAGDRHFAEFM 156
            AILA+H E  +L+K++ IY++GQPR GD  F EFM
Sbjct: 304 PAILAIHGEDELLDKLEGIYTFGQPRVGDEDFGEFM 335

[Close]

BLAST of Bhi09G002726 vs. ExPASy Swiss-Prot
Match: A0A1S3ZP85 (Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 189.5 bits (480), Expect = 4.6e-47
Identity = 115/337 (34.12%), Postives = 160/337 (47.48%), Query Frame = 0


Query: 1   FQNRATTQAFMF-QTTSDPNIIVVAFRGTSPLDAFDWQVNVDFSWYDIHGVGRIHSGFMK 60
           F+   +TQ F+      D N+I+V+FRGT P DA DW  + D+SWY+I  +G++H GF++
Sbjct: 227 FEKEMSTQVFLLCDKPKDANLILVSFRGTEPFDADDWITDFDYSWYEIPKLGKVHMGFLE 286

Query: 61  ALGLQKRKGW-------------------------PKELIPTTTT--------------- 120
           ALGL  R                            P E   ++T+               
Sbjct: 287 ALGLGNRTNASTFHEQLFVNNLKFANLENVHATIPPSESSKSSTSFSDSDAHTGSDLSSD 346

Query: 121 ----------------PRQFAYYTLRQKLFDIAKTNANAKFILTGHSLGAALAILFVAIL 180
                           P + AYY +R KL  + K + NAKF++TGHSLG ALAILF A+L
Sbjct: 347 SERPTDTRKKKFRLEIPERTAYYVVRSKLKRLLKEHKNAKFVVTGHSLGGALAILFPAVL 406

Query: 181 ALHDESLVLEKIQAIYSYGQPRAGDRHFAEFM---------------------------- 232
            LH+E  V+E++  IY+YGQPR G+R    FM                            
Sbjct: 407 VLHEEVDVMERLLGIYTYGQPRVGNRQLGRFMEAHLEHPVPKYFRVVYCNDLVPRLPYDN 466

[Close]

BLAST of Bhi09G002726 vs. ExPASy Swiss-Prot
Match: F4JFU8 (Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 1.9e-37
Identity = 94/298 (31.54%), Postives = 148/298 (49.66%), Query Frame = 0


Query: 1   FQNRATTQAFMF-QTTSDPNIIVVAFRGTSPLDAFDWQVNVDFSWYDIHGVGRIHSGFMK 60
           +Q + +TQ F+F     D N+IV++FRGT P DA DW  + D+SWY++  VG++H GF++
Sbjct: 214 YQKQMSTQVFVFTDKQKDANLIVISFRGTEPFDADDWGTDFDYSWYEVPNVGKLHMGFLE 273

Query: 61  ALGLQKRKG---WPKELIPTTTTPRQ------------FAYYTLRQKLFDIAKTNANAKF 120
           A+GL  R     +   L   T++  +             AYY +R  L  +   + NA+F
Sbjct: 274 AMGLGNRDDTTTFHYNLFEQTSSEEENSKKNLLDMVERSAYYAVRVILKRLLSEHENARF 333

Query: 121 ILTGHSLGAALAILFVAILALHDESLVLEKIQAIYSYGQPRAGDRHFAEFM--------- 180
           ++TGHSLG ALAILF  +L L++E+ +++++  +Y++GQPR G+R    FM         
Sbjct: 334 VVTGHSLGGALAILFPTLLVLNEETEIMKRLLGVYTFGQPRIGNREVGLFMKAQLNQPVD 393

Query: 181 ----------------------------------------IVKEQPNKNYFSLLWLMPKY 234
                                                     +++P+ N + L + +  +
Sbjct: 394 RYFRVVYCNDIVPRLPYDDKTFLYKHFGLCLFYDSFYNETKAEDEPDPNPYGLRYKILGH 453

[Close]

BLAST of Bhi09G002726 vs. ExPASy Swiss-Prot
Match: Q5VKJ7 (Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 6.2e-36
Identity = 95/302 (31.46%), Postives = 140/302 (46.36%), Query Frame = 0


Query: 1   FQNRATTQAFMF-QTTSDPNIIVVAFRGTSPLDAFDWQVNVDFSWYDIHGVGRIHSGFMK 60
           FQ+   T AF+F     D N+IV++FRGT P    +W  + DFS   +   G +H GF++
Sbjct: 209 FQDARNTHAFIFCDKPKDANLIVISFRGTGPFSIPNWCTDFDFSLVGLGDAGSVHVGFLE 268

Query: 61  ALGLQKRKGWPK-ELIPTTTTPRQF--------------------AYYTLRQKLFDIAKT 120
           A+GL  R      E    T +P                        Y+     L  + K 
Sbjct: 269 AMGLGHRNSISSFETSINTKSPGSITELRKESEMAPDHLVWAYDGVYFLAASTLKGLLKD 328

Query: 121 NANAKFILTGHSLGAALAILFVAILALHDESLVLEKIQAIYSYGQPRAGDRHFAEFM--- 180
           + NAKF++TGHSLG ALAILF  IL +  E+ VL+++  +Y++GQPR G+ +   FM   
Sbjct: 329 HKNAKFVVTGHSLGGALAILFTCILEIQQETEVLDRLLNVYTFGQPRIGNYNLGYFMQNR 388

Query: 181 ----------------------------------------------IVKEQPNKNYFSLL 232
                                                           KE+P++N F + 
Sbjct: 389 LNFPERRYFRVVYCNDMVPRVPFDDVFFTFEHFGTCIYYDSRFFGYFTKEEPSRNPFGIE 448

[Close]

BLAST of Bhi09G002726 vs. ExPASy Swiss-Prot
Match: O59952 (Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 2.7e-07
Identity = 45/142 (31.69%), Postives = 73/142 (51.41%), Query Frame = 0


Query: 20  IIVVAFRGTSPLDAFDWQVNVDFSWYDIHGV---GRIHSGFMKALGLQKRKGWPKELIPT 79
           +IV++FRG+  ++  +W  N++F   +I+ +    R H GF                   
Sbjct: 97  LIVLSFRGSRSIE--NWIGNLNFDLKEINDICSGCRGHDGF------------------- 156

Query: 80  TTTPRQFAYYTLRQKLFDIAKTNANAKFILTGHSLGAALAILFVAILALHDESLVLEKIQ 139
           T++ R  A  TLRQK+ D  + + + + + TGHSLG ALA +  A L  +   +      
Sbjct: 157 TSSWRSVA-DTLRQKVEDAVREHPDYRVVFTGHSLGGALATVAGADLRGNGYDI------ 210

Query: 140 AIYSYGQPRAGDRHFAEFMIVK 159
            ++SYG PR G+R FAEF+ V+
Sbjct: 217 DVFSYGAPRVGNRAFAEFLTVQ 210

[Close]

BLAST of Bhi09G002726 vs. ExPASy Swiss-Prot
Match: P19515 (Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2)

HSP 1 Score: 49.7 bits (117), Expect = 5.7e-05
Identity = 44/153 (28.76%), Postives = 72/153 (47.06%), Query Frame = 0


Query: 7   TQAFMFQTTSDPNIIVVAFRGTSPLDAFDWQVNVDF---SWYDIHGVGRIHSGFMKALGL 66
           T A + +  S+  I +V FRG+S +   +W  ++ F   S+  + G  ++H GF+ + G 
Sbjct: 156 TNAMVARGDSEKTIYIV-FRGSSSIR--NWIADLTFVPVSYPPVSGT-KVHKGFLDSYGE 215

Query: 67  QKRKGWPKELIPTTTTPRQFAYYTLRQKLFDIAKTNANAKFILTGHSLGAALAILFVAIL 126
            +      EL+ T               + D  K   + K  +TGHSLG A A+L    L
Sbjct: 216 VQ-----NELVAT---------------VLDQFKQYPSYKVAVTGHSLGGATALLCALDL 275

Query: 127 ALHDESLVLEKIQAIYSYGQPRAGDRHFAEFMI 157
              +E L    +  +Y+ GQPR GD  FA +++
Sbjct: 276 YQREEGLSSSNL-FLYTQGQPRVGDPAFANYVV 283

[Close]

BLAST of Bhi09G002726 vs. ExPASy TrEMBL
Match: A0A5D3D9E9 (Lipase, class 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G003320 PE=4 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 2.6e-122
Identity = 230/300 (76.67%), Postives = 239/300 (79.67%), Query Frame = 0


Query: 1   FQNRATTQAFMFQTT-SDPNIIVVAFRGTSPLDAFDWQVNVDFSWYDIHGVGRIHSGFMK 60
           FQ+RATTQAFMFQTT SDPNI VVAFRGT+PLDA+DWQVNVDFSWYDIHGVGRIHSGFMK
Sbjct: 185 FQSRATTQAFMFQTTASDPNITVVAFRGTTPLDAYDWQVNVDFSWYDIHGVGRIHSGFMK 244

Query: 61  ALGLQKRKGWPKELIPTTTTPRQFAYYTLRQKLFDIAKTNANAKFILTGHSLGAALAILF 120
           ALGLQKRKGWPKEL PTTT+  QFAYYTLRQKL DIAKTNANA+FILTGHSLGAALA+LF
Sbjct: 245 ALGLQKRKGWPKELTPTTTSAPQFAYYTLRQKLIDIAKTNANARFILTGHSLGAALAVLF 304

Query: 121 VAILALHDESLVLEKIQAIYSYGQPRAGDRHFAEFM------------------------ 180
           VAILALHDESLVLEKI+AIYSYGQPR GDRHFAEFM                        
Sbjct: 305 VAILALHDESLVLEKIKAIYSYGQPRVGDRHFAEFMVSVIKKYNFEYHRYVYFSDLVPRL 364

Query: 181 -------------------------IVKEQPNKNYFSLLWLMPKYLNAWLELVRSFVIPF 240
                                    IVK QPNKNYFSLLWL+PKYLNAWLELVRSF+IPF
Sbjct: 365 PADGILFKYKHFGRCIYFDSLYRGRIVKGQPNKNYFSLLWLIPKYLNAWLELVRSFLIPF 424

Query: 241 VKGYDYYESLLMKMVRFIGLFMPGLTAHIPTDYVNSTRLGKLNVSEEILEDGDDCIEPDY 251
           VKGYDYYESL MK VRFIGL MPGLTAHIPTDYVNSTRLGKLNV EEILEDGDDCIEPDY
Sbjct: 425 VKGYDYYESLAMKGVRFIGLLMPGLTAHIPTDYVNSTRLGKLNVPEEILEDGDDCIEPDY 484

[Close]

BLAST of Bhi09G002726 vs. ExPASy TrEMBL
Match: A0A6J1FE49 (uncharacterized protein LOC111443148 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443148 PE=4 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 7.2e-120
Identity = 227/300 (75.67%), Postives = 237/300 (79.00%), Query Frame = 0


Query: 1   FQNRATTQAFMFQ-TTSDPNIIVVAFRGTSPLDAFDWQVNVDFSWYDIHGVGRIHSGFMK 60
           FQNRATTQAFMFQ T SDPNIIVVAFRGTSPLDAFDWQVNVDFSWYDIHGVGRIHSGFMK
Sbjct: 185 FQNRATTQAFMFQNTASDPNIIVVAFRGTSPLDAFDWQVNVDFSWYDIHGVGRIHSGFMK 244

Query: 61  ALGLQKRKGWPKELIPTTTTPRQFAYYTLRQKLFDIAKTNANAKFILTGHSLGAALAILF 120
           ALGLQKRKGWPKELIP+TTT  QFAYYTLRQKL DIAK+NA+A+FI TGHSLGAALAILF
Sbjct: 245 ALGLQKRKGWPKELIPSTTTTTQFAYYTLRQKLIDIAKSNADARFIFTGHSLGAALAILF 304

Query: 121 VAILALHDESLVLEKIQAIYSYGQPRAGDRHFAEFM------------------------ 180
           VAIL LHDES VLEK+QAIYSYGQPRAGDRHFAEFM                        
Sbjct: 305 VAILGLHDESTVLEKLQAIYSYGQPRAGDRHFAEFMVSIIQKYNFEYHRYVYFSDLVPRL 364

Query: 181 -------------------------IVKEQPNKNYFSLLWLMPKYLNAWLELVRSFVIPF 240
                                    IVKEQPNKNYFSLLWL+PKYLNAWLEL+RSFVIPF
Sbjct: 365 PADGIIFKYKHFGSCIYFDSLYRGRIVKEQPNKNYFSLLWLVPKYLNAWLELIRSFVIPF 424

Query: 241 VKGYDYYESLLMKMVRFIGLFMPGLTAHIPTDYVNSTRLGKLNVSEEILEDGDDCIEPDY 251
           VKGYDYYESL MK VRFIGLF+PGLTAHIPTDYVNSTRLGKLNV +EIL + D CIEPDY
Sbjct: 425 VKGYDYYESLPMKGVRFIGLFIPGLTAHIPTDYVNSTRLGKLNVPQEILGNEDYCIEPDY 484

[Close]

BLAST of Bhi09G002726 vs. ExPASy TrEMBL
Match: A0A6J1IEH0 (uncharacterized protein LOC111476506 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111476506 PE=4 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 9.4e-120
Identity = 227/300 (75.67%), Postives = 236/300 (78.67%), Query Frame = 0


Query: 1   FQNRATTQAFMFQ-TTSDPNIIVVAFRGTSPLDAFDWQVNVDFSWYDIHGVGRIHSGFMK 60
           FQNRATTQAFMFQ T SDPNIIVVAFRGTSPLDAFDWQVNVDFSWYDIHGVGRIHSGFMK
Sbjct: 185 FQNRATTQAFMFQNTASDPNIIVVAFRGTSPLDAFDWQVNVDFSWYDIHGVGRIHSGFMK 244

Query: 61  ALGLQKRKGWPKELIPTTTTPRQFAYYTLRQKLFDIAKTNANAKFILTGHSLGAALAILF 120
           ALGLQKRKGWPKELIP+TTT  QFAYYTLRQKL DIAK+NANA+FI TGHSLGAALAILF
Sbjct: 245 ALGLQKRKGWPKELIPSTTTTTQFAYYTLRQKLIDIAKSNANARFIFTGHSLGAALAILF 304

Query: 121 VAILALHDESLVLEKIQAIYSYGQPRAGDRHFAEFM------------------------ 180
           VAIL LHDES VLEK+QAIYSYGQPRAGDRHFAEFM                        
Sbjct: 305 VAILGLHDESTVLEKLQAIYSYGQPRAGDRHFAEFMVSIIQKYNFEYHRYVYFSDLVPRL 364

Query: 181 -------------------------IVKEQPNKNYFSLLWLMPKYLNAWLELVRSFVIPF 240
                                    IVKEQPNKNYFSLLWL+PKYLNAWLEL+RSFVIPF
Sbjct: 365 PADGIIFKYKHFGSCIYFDSLYRGRIVKEQPNKNYFSLLWLVPKYLNAWLELIRSFVIPF 424

Query: 241 VKGYDYYESLLMKMVRFIGLFMPGLTAHIPTDYVNSTRLGKLNVSEEILEDGDDCIEPDY 251
           VKGYDYYESL MK VRFIGLF+PGLTAH PTDYVNSTRLGKLNV +EIL + D CIEPDY
Sbjct: 425 VKGYDYYESLPMKGVRFIGLFIPGLTAHSPTDYVNSTRLGKLNVPQEILGNEDYCIEPDY 484

[Close]

BLAST of Bhi09G002726 vs. ExPASy TrEMBL
Match: A0A0A0L0A6 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639860 PE=4 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 3.6e-119
Identity = 223/300 (74.33%), Postives = 237/300 (79.00%), Query Frame = 0


Query: 1   FQNRATTQAFMFQTT-SDPNIIVVAFRGTSPLDAFDWQVNVDFSWYDIHGVGRIHSGFMK 60
           FQ+RA+TQAF+FQTT SDPN  VVAFRGT+PLDA+DWQVN+DFSWY+IHGVGRIHSGFMK
Sbjct: 185 FQSRASTQAFLFQTTVSDPNTTVVAFRGTTPLDAYDWQVNLDFSWYEIHGVGRIHSGFMK 244

Query: 61  ALGLQKRKGWPKELIPTTTTPRQFAYYTLRQKLFDIAKTNANAKFILTGHSLGAALAILF 120
           ALGLQKRKGWPKEL PTTT   QFAYYTLRQKL DIAKTNANA+FI TGHSLGAALA+LF
Sbjct: 245 ALGLQKRKGWPKELTPTTTGAPQFAYYTLRQKLIDIAKTNANARFIFTGHSLGAALAVLF 304

Query: 121 VAILALHDESLVLEKIQAIYSYGQPRAGDRHFAEFM------------------------ 180
           VAILALHDESLVLEKI+AIYSYGQPR GDRHFAEFM                        
Sbjct: 305 VAILALHDESLVLEKIKAIYSYGQPRVGDRHFAEFMVSIIKKYNFEYYRYVYFSDLVPRL 364

Query: 181 -------------------------IVKEQPNKNYFSLLWLMPKYLNAWLELVRSFVIPF 240
                                    IVKEQPNKNYFSLLWL+PKY+NAWLELVRSF+IPF
Sbjct: 365 PADGILFKYKHFGRCIYFDSLYRGRIVKEQPNKNYFSLLWLIPKYVNAWLELVRSFLIPF 424

Query: 241 VKGYDYYESLLMKMVRFIGLFMPGLTAHIPTDYVNSTRLGKLNVSEEILEDGDDCIEPDY 251
           VKGYDYYESL MK VRFIGL MPGLTAHIPTDYVNSTRLGKL+V EEILEDGDDCIEPDY
Sbjct: 425 VKGYDYYESLAMKGVRFIGLLMPGLTAHIPTDYVNSTRLGKLSVPEEILEDGDDCIEPDY 484

[Close]

BLAST of Bhi09G002726 vs. ExPASy TrEMBL
Match: A0A6J1CEI9 (uncharacterized protein LOC111010491 OS=Momordica charantia OX=3673 GN=LOC111010491 PE=4 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 2.8e-92
Identity = 177/291 (60.82%), Postives = 204/291 (70.10%), Query Frame = 0


Query: 1   FQNRATTQAFMFQ-TTSDPNIIVVAFRGTSPLDAFDWQVNVDFSWYDIHGVGRIHSGFMK 60
           FQ +ATTQAFMFQ T +DPN+ VVAFRGTSPLDA+DWQV+ DFSWYDI GVGRIHSGFMK
Sbjct: 187 FQGKATTQAFMFQNTAADPNVTVVAFRGTSPLDAYDWQVDADFSWYDIEGVGRIHSGFMK 246

Query: 61  ALGLQKRKGWPKELIPTTTTPRQFAYYTLRQKLFDIAKTNANAKFILTGHSLGAALAILF 120
           ALGLQK  GWP+EL+    +   FAYYTLRQKL DIAK+N NAKFI+TGHSLG ALA LF
Sbjct: 247 ALGLQKATGWPEELV---KSKHHFAYYTLRQKLRDIAKSNDNAKFIITGHSLGGALAALF 306

Query: 121 VAILALHDESLVLEKIQAIYSYGQPRAGDRHFAEFM------------------------ 180
           V +L+LH +S VLEK+QA+Y+Y QPR GDR FAEFM                        
Sbjct: 307 VTVLSLHRDSAVLEKLQAVYTYSQPRVGDRRFAEFMVNTVKKYGFKYHRYVYSSDLVPRV 366

Query: 181 -------------------------IVKEQPNKNYFSLLWLMPKYLNAWLELVRSFVIPF 240
                                    IVKE+PNKNYFSL+W+MPKYLNAW EL+RSF++P 
Sbjct: 367 PFDGVIFKYKHFGRCIYFNSLYKGRIVKEEPNKNYFSLIWVMPKYLNAWWELIRSFIVPL 426

Query: 241 VKGYDYYESLLMKMVRFIGLFMPGLTAHIPTDYVNSTRLGKLNVSEEILED 242
           VKG+DY+ESLLMK  R +GLF+PGL AHIPTDYVNS RLGK N   +  ED
Sbjct: 427 VKGFDYHESLLMKGARVVGLFIPGLAAHIPTDYVNSIRLGKFNPPPQEFED 474

The following BLAST results are available for this feature:

BLAST of Bhi09G002726 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G42930.1	4.7e-63	42.61	alpha/beta-Hydrolases superfamily protein	[more]
AT1G45201.1	1.5e-61	44.52	triacylglycerol lipase-like 1	[more]
AT1G56630.1	1.4e-54	39.29	alpha/beta-Hydrolases superfamily protein	[more]
AT5G67050.1	1.6e-50	38.25	alpha/beta-Hydrolases superfamily protein	[more]
AT1G45201.2	1.1e-43	55.77	triacylglycerol lipase-like 1	[more]

BLAST of Bhi09G002726 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A1S3ZP85	4.6e-47	34.12	Triacylglycerol lipase OBL1 OS=Nicotiana tabacum OX=4097 GN=OBL1 PE=1 SV=1	[more]
F4JFU8	1.9e-37	31.54	Triacylglycerol lipase OBL1 OS=Arabidopsis thaliana OX=3702 GN=OBL1 PE=1 SV=1	[more]
Q5VKJ7	6.2e-36	31.46	Triacylglycerol lipase OBL1 OS=Ricinus communis OX=3988 GN=OBL1 PE=1 SV=1	[more]
O59952	2.7e-07	31.69	Lipase OS=Thermomyces lanuginosus OX=5541 GN=LIP PE=1 SV=1	[more]
P19515	5.7e-05	28.76	Lipase OS=Rhizomucor miehei OX=4839 PE=1 SV=2	[more]

BLAST of Bhi09G002726 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3D9E9	2.6e-122	76.67	Lipase, class 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold134G0033...	[more]
A0A6J1FE49	7.2e-120	75.67	uncharacterized protein LOC111443148 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1IEH0	9.4e-120	75.67	uncharacterized protein LOC111476506 isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A0A0L0A6	3.6e-119	74.33	Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639860 PE...	[more]
A0A6J1CEI9	2.8e-92	60.82	uncharacterized protein LOC111010491 OS=Momordica charantia OX=3673 GN=LOC111010...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 1..169 e-value: 9.3E-33 score: 115.9
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 9..155
IPR002921	Fungal lipase-like domain	PFAM	PF01764	Lipase_3	coord: 22..166 e-value: 2.9E-26 score: 92.0
None	No IPR available	PANTHER	PTHR46086:SF4	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 1..155 coord: 157..236
None	No IPR available	CDD	cd00519	Lipase_3	coord: 1..167 e-value: 4.08863E-26 score: 99.8582
IPR044819	Triacylglycerol lipase OBL1-like	PANTHER	PTHR46086	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 157..236 coord: 1..155

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi09M002726	Bhi09M002726	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006629	lipid metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004806	triglyceride lipase activity