Bhi09G002683 (gene) Wax gourd (B227) v1

BLAST of Bhi09G002683 vs. TAIR 10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )

HSP 1 Score: 440.7 bits (1132), Expect = 1.6e-123
Identity = 237/504 (47.02%), Postives = 323/504 (64.09%), Query Frame = 0

Query: 7   IPFLYLLVLFILQIFTNHFLHRIRNLPPSPYLSLPIIGQLYLLKSPLHRTLSEIAHRHG- 66
           I  L  L LF L IF      R  NLPPSP +SLP+IG L+LLK PLHRT   ++   G 
Sbjct: 6   ILILSFLSLFTLAIFLLTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKSIGN 65

Query: 67  -PILFFRFGFRQVLVVSSPSAAEDCLSKNDVVFANRPRLFSGKYLGYNYTSLLWAPYGEH 126
            P+   R G R V V+SS S AE+C +KNDVV ANRP+    K+LGYN T LL A YG+H
Sbjct: 66  APVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYGDH 125

Query: 127 WRNLRRISSLEILSKHRLQTLSSIRVDEVRTLIRRLYNAKND---VVDMRTEFFELMFNI 186
           WRNLRRI+++EI S HRL +   IR DE+R LI  L          V+M+T    L  N 
Sbjct: 126 WRNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHGFVEVEMKTLLTNLASNT 185

Query: 187 MMRMIAGKRYYGEDVAADTEEVGRFREIQEETFRLSSKTNLGDFLRVVKWVGLSKGLENK 246
            +RM+AGKRY+GE    D ++    + +  E    +   N  D+L +++WV      E +
Sbjct: 186 TIRMLAGKRYFGE----DNDDAKLVKNLVSEAVTSAGAGNPIDYLSILRWV---SSYEKR 245

Query: 247 LRELQMKRDVWMQSLIEEHRKQMNNTSTSLQNDAKKTMIEVLLSLQQMEPHYYKDEFIRG 306
           ++ L  + D ++Q L++E R          + +  +TMI+ LL+LQ ++P YY D  I+G
Sbjct: 246 IKNLGNRFDTFLQKLVDEKR---------AEKEKGETMIDHLLALQDIQPDYYTDVIIKG 305

Query: 307 LMLVLLLAGTEGSINTMEWLLSLLLNHPDCLQRAQMEIDDVVGRSNRILEESDLAHLPYL 366
           ++L L++AGT+ S  T+EW +S LLNHP+ L++A+MEID+ VG  +R+++ESD+ +L YL
Sbjct: 306 IILTLIIAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVG-LDRLVDESDIVNLSYL 365

Query: 367 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFCVPAGTMLFVNVWAIQNDPTVWVEPGNFN 426
           +S++ ETLRMYP  PLL+PH SSEDC VGG+ +P+GTM+  N WA+  DP VW +P  F 
Sbjct: 366 QSIVLETLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFK 425

Query: 427 PERFGSEGEGFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFDWESVGGECVDMREGG 486
           PERF  EGE  K + FG GRR CPG GL  R++  A+GSL+QCF+WE VG + VDM E  
Sbjct: 426 PERFEKEGEAEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDMTEDK 485

Query: 487 GLTLPKALPLHALCRPRSNVAHLL 506
           G TLPKA+PL A+C+ RS V  L+
Sbjct: 486 GATLPKAIPLRAMCKARSIVDKLI 492

BLAST of Bhi09G002683 vs. TAIR 10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )

HSP 1 Score: 439.9 bits (1130), Expect = 2.8e-123
Identity = 235/503 (46.72%), Postives = 321/503 (63.82%), Query Frame = 0

Query: 9   FLYLLVLFILQIFTNHFLHRIRNLPPSPYLSLPIIGQLYLLKSPLHRTLSEIAHRHG--P 68
           F +L +   L       + R  NLPPSP  +LP+IG L LLK PLHR    ++   G  P
Sbjct: 8   FTFLFISLSLTFIIGR-IKRRPNLPPSPSWALPVIGHLRLLKPPLHRVFLSVSESLGDAP 67

Query: 69  ILFFRFGFRQVLVVSSPSAAEDCLSKNDVVFANRPRLFSGKYLGYNYTSLLWAPYGEHWR 128
           I+  R G R V VVSS S AE+C +KNDVV ANR    + K++ Y  T+++ A YG+HWR
Sbjct: 68  IISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTASYGDHWR 127

Query: 129 NLRRISSLEILSKHRLQTLSSIRVDEVRTLI---RRLYNAKNDVVDMRTEFFELMFNIMM 188
           NLRRI ++EI S HRL + SSIR DE+  LI    R  + +   V+M++ F  L FN ++
Sbjct: 128 NLRRIGAVEIFSAHRLNSFSSIRRDEIHRLIACLSRNSSLEFTKVEMKSMFSNLTFNNII 187

Query: 189 RMIAGKRYYGEDVAADTEEVGRFREIQEETFRLSSKTNLGDFLRVVKWVGLSKGLENKLR 248
           RM+AGK YYG D A D  E  R RE+  E        N  D+L ++ W+    G E +++
Sbjct: 188 RMLAGKCYYG-DGAEDDPEAKRVRELIAEGMGCFGAGNTADYLPILTWI---TGSEKRIK 247

Query: 249 ELQMKRDVWMQSLIEEHRKQMNNTSTSLQNDAKKTMIEVLLSLQQMEPHYYKDEFIRGLM 308
           ++  + D ++Q L++E R+         +   + TM++ LL LQ+ +P YY D  I+G+M
Sbjct: 248 KIASRLDEFLQGLVDERREG--------KEKRQNTMVDHLLCLQETQPEYYTDNIIKGIM 307

Query: 309 LVLLLAGTEGSINTMEWLLSLLLNHPDCLQRAQMEIDDVVGRSNRILEESDLAHLPYLRS 368
           L L+LAGT+ S  T+EW LS LLNHP  L +A+ EID+ VG  NR++EESDL+HLPYL++
Sbjct: 308 LSLILAGTDTSAVTLEWTLSALLNHPQILSKARDEIDNKVG-LNRLVEESDLSHLPYLQN 367

Query: 369 LIHETLRMYPPGPLLIPHESSEDCHVGGFCVPAGTMLFVNVWAIQNDPTVWVEPGNFNPE 428
           ++ E+LR+YP  PLL+PH +SEDC VGG+ +P GTML  N WAI  DP +W +P +F PE
Sbjct: 368 IVSESLRLYPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTSFKPE 427

Query: 429 RFGSEGEGFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFDWESVGGECVDMRE-GGG 488
           RF  EGE  K + FG GRR CPG GL  R+  L +GSLIQCF+WE +G E VDM E GGG
Sbjct: 428 RFEKEGEAQKLLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEVDMTEGGGG 487

Query: 489 LTLPKALPLHALCRPRSNVAHLL 506
           + +PKA+PL A+C+ R  V  +L
Sbjct: 488 VIMPKAIPLVAMCKARPVVGKIL 496

BLAST of Bhi09G002683 vs. TAIR 10
Match: AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )

HSP 1 Score: 439.5 bits (1129), Expect = 3.7e-123
Identity = 234/502 (46.61%), Postives = 323/502 (64.34%), Query Frame = 0

Query: 5   MAIPFLYLLVLFILQIFTNHFLHRIRNLPPSPYLSLPIIGQLYLLKSPLHRTLSEIAHRH 64
           + + FL+L +   L +  +   +R  NLPPSP   LP+IG L+LLK P+HRT   I+   
Sbjct: 7   LLLSFLFLTISIKLLLTKS---NRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHSISKSL 66

Query: 65  G--PILFFRFGFRQVLVVSSPSAAEDCLSKNDVVFANRPRLFSGKYLGYNYTSLLWAPYG 124
           G  PI   R G R V V+SS S AE+C +KNDVV ANRP +   K++GYN+T+++ A YG
Sbjct: 67  GNAPIFHLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAASYG 126

Query: 125 EHWRNLRRISSLEILSKHRLQTLSSIRVDEVRTLIRRLYNAKND---VVDMRTEFFELMF 184
           +HWRNLRRI+++EI S HR+ T SSIR DE+R LI  L          V++++    L F
Sbjct: 127 DHWRNLRRIAAVEIFSSHRISTFSSIRKDEIRRLITHLSRDSLHGFVEVELKSLLTNLAF 186

Query: 185 NIMMRMIAGKRYYGEDVAADTEEVGRFREIQEETFRLSSKTNLGDFLRVVKWVGLSKGLE 244
           N ++ M+AGKRYYG     D +E    RE+  E    +   NL D+L  + WV      E
Sbjct: 187 NNIIMMVAGKRYYGTG-TEDNDEAKLVRELIAEIMAGAGSGNLADYLPSINWV---TNFE 246

Query: 245 NKLRELQMKRDVWMQSLIEEHRKQMNNTSTSLQNDAKKTMIEVLLSLQQMEPHYYKDEFI 304
           N+ + L  + D  +Q L++E R          + +  +T+I+ LLS Q+ EP YY D  I
Sbjct: 247 NQTKILGNRLDRVLQKLVDEKR---------AEKEKGQTLIDHLLSFQETEPEYYTDVII 306

Query: 305 RGLMLVLLLAGTEGSINTMEWLLSLLLNHPDCLQRAQMEIDDVVGRSNRILEESDLAHLP 364
           +G++L L+LAGT+ S  T+EW +S LLNHP+ L++A+ EIDD +G S+R++EESD+ +L 
Sbjct: 307 KGIILALVLAGTDTSSVTLEWAMSNLLNHPEILEKARAEIDDKIG-SDRLVEESDIVNLH 366

Query: 365 YLRSLIHETLRMYPPGPLLIPHESSEDCHVGGFCVPAGTMLFVNVWAIQNDPTVWVEPGN 424
           YL++++ ETLR+YP  PLL+PH SS++C V G+ +P  T+L  NVWA+  DP +W EP  
Sbjct: 367 YLQNIVSETLRLYPAVPLLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPER 426

Query: 425 FNPERFGSEGEGFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFDWESVGGECVDMRE 484
           F PERF  EGE  K MPFG GRR CPG  LG R+V LA+G LIQ F+WE VG E VDM E
Sbjct: 427 FKPERFEKEGEARKLMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELVDMTE 486

Query: 485 GGGLTLPKALPLHALCRPRSNV 502
           G G+T+PKA PL A+C+ R+ V
Sbjct: 487 GEGITMPKATPLRAMCKARAIV 491

BLAST of Bhi09G002683 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 439.1 bits (1128), Expect = 4.8e-123
Identity = 234/508 (46.06%), Postives = 326/508 (64.17%), Query Frame = 0

Query: 4   SMAIPFLYLLVLFILQIFTNHFLHRIRNLPPSPYLSLPIIGQLYLLKSPLHRTLSEIAHR 63
           ++ + F +  ++  L IF    + R  NLPPSP  +LP+IG L LLK PLHR    ++  
Sbjct: 3   ALMLIFTFCFIVLSL-IFLIGRIKRKLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQS 62

Query: 64  HG--PILFFRFGFRQVLVVSSPSAAEDCLSKNDVVFANRPRLFSGKYLGYNYTSLLWAPY 123
            G  PI+  R G R + VVSS S AE+C +KNDV+ ANR    S K++ Y  ++++ A Y
Sbjct: 63  LGDAPIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASY 122

Query: 124 GEHWRNLRRISSLEILSKHRLQTLSSIRVDEVRTLIRRLYNAKN---DVVDMRTEFFELM 183
            EHWRNLRRI +LEI S HRL + SSIR DE+R LI RL    +     V+M++ F +L 
Sbjct: 123 SEHWRNLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTKVEMKSMFSDLT 182

Query: 184 FNIMMRMIAGKRYYGEDVAADTEEVGRFREIQEETFRLSSKTNLGDFLRVVKWVGLSKGL 243
           FN ++RM+AGK YYG D   D  E  R R +  E    S   N  D++ ++ W+  S   
Sbjct: 183 FNNIIRMLAGKCYYG-DGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYS--- 242

Query: 244 ENKLRELQMKRDVWMQSLIEEHRKQMNNTSTSLQNDAKKTMIEVLLSLQQMEPHYYKDEF 303
           E ++++L  + D ++Q L++E R+         +   + TM++ LL LQ+ +P YY D  
Sbjct: 243 ETRIKKLAGRLDEFLQGLVDEKREG--------KEKKENTMVDHLLCLQETQPEYYMDRI 302

Query: 304 IRGLMLVLLLAGTEGSINTMEWLLSLLLNHPDCLQRAQMEIDDVVGRSNRILEESDLAHL 363
           I+G ML L+  GT+ +  T+EW LS LLN+P+ L +A+ EID ++G  +R+LEESD+ +L
Sbjct: 303 IKGTMLSLIAGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIG-VDRLLEESDIPNL 362

Query: 364 PYLRSLIHETLRMYPPGPLLIPHESSEDCHVGGFCVPAGTMLFVNVWAIQNDPTVWVEPG 423
           PYL++++ ETLR+YP  P+L+PH +S+DC VGG+ +P GTML  N WAI  DP +W +P 
Sbjct: 363 PYLQNIVSETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPT 422

Query: 424 NFNPERFGSEGEGFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFDWESVGGECVDMR 483
           +F PERF  EGE  K MPFG GRR CPG GL  R+V L++GSLIQCF+WE +G E VDM 
Sbjct: 423 SFKPERFEKEGEAKKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEVDMT 482

Query: 484 EGGGLTLPKALPLHALCRPRSNVAHLLP 507
           EG GLT+PKA PL A+CR R  V  +LP
Sbjct: 483 EGPGLTMPKARPLEAMCRARDFVGKILP 496

BLAST of Bhi09G002683 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 439.1 bits (1128), Expect = 4.8e-123
Identity = 231/498 (46.39%), Postives = 325/498 (65.26%), Query Frame = 0

Query: 12  LLVLFILQIFTNHFLHRIRNLPPSPYLSLPIIGQLYLLKSPLHRTLSEIAH--RHGPILF 71
           +L + +  I+    L R  NLPPSP  SLP+IG L LLK P+HRT   ++    + PI  
Sbjct: 10  ILFVVLSLIYLIGKLKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNNAPIFS 69

Query: 72  FRFGFRQVLVVSSPSAAEDCLSKNDVVFANRPRLFSGKYLGYNYTSLLWAPYGEHWRNLR 131
            R G R V V SS S AE+C +KNDVV ANRP     K++ Y+YT+++ A YG+HWRNLR
Sbjct: 70  LRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHWRNLR 129

Query: 132 RISSLEILSKHRLQTLSSIRVDEVRTLIRRL---YNAKNDVVDMRTEFFELMFNIMMRMI 191
           RI S+EI S HRL +  SIR DE+R L+ RL   ++ +   VDM++   +L FN ++RM+
Sbjct: 130 RIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNNILRMV 189

Query: 192 AGKRYYGEDVAADTEEVGRFREIQEETFRLSSKTNLGDFLRVVKWVGLSKGLENKLRELQ 251
           AGKRYYG+ V  D  E  R R++  +    +   N  D+L V++   L    E ++++L 
Sbjct: 190 AGKRYYGDGV-EDDPEAKRVRQLIADVVACAGAGNAVDYLPVLR---LVSDYETRVKKLA 249

Query: 252 MKRDVWMQSLIEEHRKQMNNTSTSLQNDAKKTMIEVLLSLQQMEPHYYKDEFIRGLMLVL 311
            + D ++Q L++E R+           +   TMI+ LL+LQ+ +P Y+ D  I+G ML L
Sbjct: 250 GRLDEFLQGLVDEKRE---------AKEKGNTMIDHLLTLQESQPDYFTDRIIKGNMLAL 309

Query: 312 LLAGTEGSINTMEWLLSLLLNHPDCLQRAQMEIDDVVGRSNRILEESDLAHLPYLRSLIH 371
           +LAGT+ S  T+EW LS +LNHPD L +A+ EID  +G  +R+++ESD+++LPYL++++ 
Sbjct: 310 ILAGTDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIG-LDRLMDESDISNLPYLQNIVS 369

Query: 372 ETLRMYPPGPLLIPHESSEDCHVGGFCVPAGTMLFVNVWAIQNDPTVWVEPGNFNPERFG 431
           ETLR+YP  P+L+PH +SEDC V G+ +P GT+L  NVWAI  DP +W +P +F PERF 
Sbjct: 370 ETLRLYPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFE 429

Query: 432 SEGEGFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFDWESVGGECVDMREGGGLTLP 491
            EGE  K MPFG GRR CPG GL  R++ L +GSLIQC +WE +G E VDM EG G+T+P
Sbjct: 430 KEGEAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGEE-VDMSEGKGVTMP 489

Query: 492 KALPLHALCRPRSNVAHL 505
           KA PL A+CR R +V  +
Sbjct: 490 KAKPLEAMCRARPSVVKI 492

BLAST of Bhi09G002683 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 5.9e-134
Identity = 248/507 (48.92%), Postives = 339/507 (66.86%), Query Frame = 0

Query: 1   MKMSMAIPFLYLLVLFILQIFTNHFLHRIRNLPPSPYLSLPIIGQLYLLKSPLHRTLSEI 60
           M+ S  +     +VL  + +       R RNLPPSP L+LP+IG L+L+   LHR+L ++
Sbjct: 1   MESSTLLYTFLAVVLLSISLKLFPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDL 60

Query: 61  AHRHGPILFFRFGFRQVLVVSSPSAAEDCLSKNDVVFANRPRLFSGKYLGYNYTSLLWAP 120
           + ++G +   + G R VLVVSSP+AAE+C +KND+VFANRP    GKY+GYNYT+++ +P
Sbjct: 61  SKKYGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSP 120

Query: 121 YGEHWRNLRRISSLEILSKHRLQTLSSIRVDEVRTLIRRLYNAKND---VVDMRTEFFEL 180
           YGEHWRNLRR++++EI S   L    SIR DEV+ L+  LY +       V+M+++  EL
Sbjct: 121 YGEHWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSEL 180

Query: 181 MFNIMMRMIAGKRYYGEDVAADTEEVGRFREIQEETFRLSSKTNLGDFLRVVKWVGLSKG 240
            FN+ MRM+AGKRY+G+DV  D++E   FR +  E F  +  +N GDF+  ++W+   K 
Sbjct: 181 SFNVTMRMVAGKRYFGQDV--DSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDF-KN 240

Query: 241 LENKLRELQMKRDVWMQSLIEEHRKQMNNTSTSLQNDAKKTMIEVLLSLQQMEPHYYKDE 300
            E K+ ++  + D ++Q LI E R   NN           TMI+ LLSLQ+ +P YY D+
Sbjct: 241 YEKKVSKISQEMDAFLQRLIHESRINKNNV----------TMIDHLLSLQESQPEYYTDQ 300

Query: 301 FIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPDCLQRAQMEIDDVVGRSNRILEESDLAH 360
            I+G+++VLLLAGT+ S  T+EW +SLLLNHP+ L++A+ EI+  VG SNR++EE DL  
Sbjct: 301 IIKGIIMVLLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVG-SNRLIEEQDLPK 360

Query: 361 LPYLRSLIHETLRMYPPGPLLIPHESSEDCHVGGFCVPAGTMLFVNVWAIQNDPTVWVEP 420
           L YL ++I ET R+ P  P+L+PHESS+DC V G+ VP GT+L VN WAI  DP  W EP
Sbjct: 361 LTYLHNIISETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEP 420

Query: 421 GNFNPERFGS-EGEGFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFDWESVGGECVD 480
             F PER G  E E  K MPFG GRR CPG GL  RVVGL +G+LIQCF+W+ +G   +D
Sbjct: 421 TLFKPERHGGVELEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKID 480

Query: 481 MREGGGLTLPKALPLHALCRPRSNVAH 504
           M EG GLT+PKA PL ALC+PR N+ H
Sbjct: 481 MAEGSGLTMPKAQPLEALCKPR-NILH 492

BLAST of Bhi09G002683 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 444.9 bits (1143), Expect = 1.2e-123
Identity = 224/501 (44.71%), Postives = 329/501 (65.67%), Query Frame = 0

Query: 11  YLLVLFILQIFTNHFLHRIRNLPPSPYLSLPIIGQLYLLKSPLHRTLSEIAHRHGPILFF 70
           YL + FI  I       + +NLPP P  SLPIIG L+ LK PLHRT   ++ ++G +   
Sbjct: 13  YLALFFIFNIVIR--ARKFKNLPPGP-PSLPIIGNLHHLKRPLHRTFKGLSEKYGHVFSL 72

Query: 71  RFGFRQVLVVSSPSAAEDCLSKNDVVFANRPRLFSGKYLGYNYTSLLWAPYGEHWRNLRR 130
            FG R V+VVSS S  + C +KNDVV ANRPR  SGKY+ YNYT+L    YGEHWRNLRR
Sbjct: 73  WFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRR 132

Query: 131 ISSLEILSKHRLQTLSSIRVDEVRTLIRRLYNAKND---VVDMRTEFFELMFNIMMRMIA 190
           I++L++LS HR+ + S IR DE + LI RL +  +     +++ +  +++ FN +MRMI+
Sbjct: 133 ITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNIMRMIS 192

Query: 191 GKRYYGEDV-AADTEEVGRFREIQEETFRLSSKTNLGDFLRVVKWVGLSKGLENKLRELQ 250
           GKRYYGED   +D +E  +FR++  E  +LS   N  DF+ +++++   + LE +L+++ 
Sbjct: 193 GKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDF-ENLEKRLKDIS 252

Query: 251 MKRDVWMQSLIEEHRKQMNNTSTSLQNDAKKTMIEVLLSLQQMEPHYYKDEFIRGLMLVL 310
            K D +++ LIEEHR          + +   TMI+ LL+LQ  +P YY D+ I+GL L +
Sbjct: 253 GKTDAFLRGLIEEHR---------TKKERANTMIDHLLNLQDSQPEYYTDQIIKGLALAM 312

Query: 311 LLAGTEGSINTMEWLLSLLLNHPDCLQRAQMEIDDVVGRSNRILEESDLAHLPYLRSLIH 370
           LLAGT+ S  T+EW +S LLNHP+ L++ + E+D  VG+ +R+++ESDL  L YL+++I+
Sbjct: 313 LLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQ-DRLVDESDLPKLTYLKNVIN 372

Query: 371 ETLRMYPPGPLLIPHESSEDCHVGGFCVPAGTMLFVNVWAIQNDPTVWVEPGNFNPERFG 430
           ETLR+Y P PLL+PH +S++C++GG+ VP  T++ +N WAI  DP +W E   F PERF 
Sbjct: 373 ETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFE 432

Query: 431 SEGEGFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFDWESVGGECVDMREGGGLTLP 490
            +GE  K + FG GRR CPGEGL +R + + +  LIQCFDW+ + G+ +D+ E  G TL 
Sbjct: 433 KKGELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDGFTLT 492

Query: 491 KALPLHALCRPRSNVAHLLPQ 508
           K +PL A+C+ R  +  +  Q
Sbjct: 493 KLVPLKAMCKSRPVINKVFKQ 499

BLAST of Bhi09G002683 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 2.7e-123
Identity = 230/501 (45.91%), Postives = 328/501 (65.47%), Query Frame = 0

Query: 3   MSMAIPFLYLLVLFILQIFTNHFLHRIRNLPPSPYLSLPIIGQLYLLKSPLHRTLSEIAH 62
           +S+ I   +L++   L++F N    + +NLPP P   LPIIG L+ LK PLH T   ++ 
Sbjct: 6   LSLIISLFFLII--TLKVFFN-TSRKFKNLPPGPQ-CLPIIGNLHQLKQPLHHTFHTLSQ 65

Query: 63  RHGPILFFRFGFRQVLVVSSPSAAEDCLSKNDVVFANRPRLFSGKYLGYNYTSLLWAPYG 122
           ++G I    FG R V+VVSS + A++C +KND+V ANRP   +GKY+GYN T++  +PYG
Sbjct: 66  KYGQIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYG 125

Query: 123 EHWRNLRRISSLEILSKHRLQTLSSIRVDEVRTLIRRL----YNAKNDVVDMRTEFFELM 182
           +HWRNLRRI S+EILS HRL +   IR DE+  LI++L    YN   + V++R  F E+ 
Sbjct: 126 DHWRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTE-VELRPMFSEMT 185

Query: 183 FNIMMRMIAGKRYYGEDV-AADTEEVGRFREIQEETFRLSSKTNLGDFLRVVKWVGLSKG 242
           FN +MRM++GKRYYG D   +D EE   FR I +E   L    N+GDFL  ++W     G
Sbjct: 186 FNTIMRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFD-G 245

Query: 243 LENKLRELQMKRDVWMQSLIEEHRKQMNNTSTSLQNDAKKTMIEVLLSLQQMEPHYYKDE 302
           LE +L+++  + D ++Q LI+EHR    N++         TMI+ LL+ QQ +P YY D+
Sbjct: 246 LEKRLKKISKRTDAFLQGLIDEHRFGKRNSN---------TMIDHLLTQQQSQPEYYTDQ 305

Query: 303 FIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPDCLQRAQMEIDDVVGRSNRILEESDLAH 362
            I+GLM+V+LLAGT+ S  T+EW +S LLNHP+ +++A+ E+D  +G  +R ++E D++ 
Sbjct: 306 IIKGLMVVMLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGH-DRQVDEHDISK 365

Query: 363 LPYLRSLIHETLRMYPPGPLLIPHESSEDCHVGGFCVPAGTMLFVNVWAIQNDPTVWVEP 422
           LPYL+S+++ETLR++   PLL+PH SSED  +GG+ +P  T+L VN W I  DP +W +P
Sbjct: 366 LPYLQSIVYETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDP 425

Query: 423 GNFNPERFGSEGEGFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFDWESVGGECVDM 482
             F PERF  EGE  K + FG GRR CPGE L  R  GL +G LIQCF+W+ +G E +DM
Sbjct: 426 TCFKPERFEKEGEVNKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDM 485

Query: 483 REGGGLTLPKALPLHALCRPR 499
            E  G+T  K   L+A+C+ R
Sbjct: 486 VEAKGITAGKKTSLNAMCKVR 490

BLAST of Bhi09G002683 vs. ExPASy Swiss-Prot
Match: Q6WNR0 (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 1.5e-121
Identity = 219/492 (44.51%), Postives = 325/492 (66.06%), Query Frame = 0

Query: 11  YLLVLFILQIFTNHFLHRIRNLPPSPYLSLPIIGQLYLLKSPLHRTLSEIAHRHGPILFF 70
           YL + FI+++       + +NLPP P  SLPIIG L+ LK PL+RT   +  ++G ++  
Sbjct: 13  YLSIFFIIRLLFQS--RKFKNLPPGP-TSLPIIGNLHHLKRPLNRTFKALTEKYGNVISL 72

Query: 71  RFGFRQVLVVSSPSAAEDCLSKNDVVFANRPRLFSGKYLGYNYTSLLWAPYGEHWRNLRR 130
            FG R V+VVSS S  ++C +KNDVV ANRPR  SGKY+ YNYT+L    YGEHWRNLRR
Sbjct: 73  WFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLRR 132

Query: 131 ISSLEILSKHRLQTLSSIRVDEVRTLIRRL---YNAKNDVVDMRTEFFELMFNIMMRMIA 190
           I+SL++LS HR+   + IR DE + LI++L    + K   V++   FF++ FN +MRMI+
Sbjct: 133 ITSLDVLSNHRINNFAPIRRDETQRLIKKLAEDSSTKFAEVELTFRFFDMTFNNIMRMIS 192

Query: 191 GKRYYGEDV-AADTEEVGRFREIQEETFRLSSKTNLGDFLRVVKWVGLSKGLENKLRELQ 250
           GKRYYG+D   ++ +E  +FR++  E  +LS   N  DF+ ++K++   + LE +++ + 
Sbjct: 193 GKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDF-ENLEKRVKRIG 252

Query: 251 MKRDVWMQSLIEEHRKQMNNTSTSLQNDAKKTMIEVLLSLQQMEPHYYKDEFIRGLMLVL 310
            K DV++  L++E R +   T+         TMI+ LL++Q+ +P YY D  I+GL L +
Sbjct: 253 EKNDVFLSGLLQEQRSKKERTN---------TMIDHLLNMQESQPEYYTDTIIKGLCLAM 312

Query: 311 LLAGTEGSINTMEWLLSLLLNHPDCLQRAQMEIDDVVGRSNRILEESDLAHLPYLRSLIH 370
           LLAGT+ S  T+EW +S +LN+P+ L++ + E+D  VG+ +R+++ESDL  L YLR++I+
Sbjct: 313 LLAGTDSSAVTLEWTMSNILNYPEVLKKVRDEVDTHVGQ-DRLVDESDLPKLTYLRNVIY 372

Query: 371 ETLRMYPPGPLLIPHESSEDCHVGGFCVPAGTMLFVNVWAIQNDPTVWVEPGNFNPERFG 430
           ETLR+Y P PLL+PH ++++C +GG+ VP  T++ +N WAI  DP  W E   F PERF 
Sbjct: 373 ETLRLYTPAPLLLPHSTADECIMGGYKVPRDTIVLINAWAIHRDPETWSEATTFKPERFD 432

Query: 431 SEGEGFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFDWESVGGECVDMREGGGLTLP 490
            +GE  K + FG GRR CPGEGL LR + + +  L+QCFDW+ +  E +DM E  G T+ 
Sbjct: 433 KKGELEKMIAFGMGRRACPGEGLALRAISMTLALLVQCFDWKRINDEKIDMSERDGFTMT 490

Query: 491 KALPLHALCRPR 499
           K LPL A+C+ R
Sbjct: 493 KLLPLKAMCKTR 490

BLAST of Bhi09G002683 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 434.9 bits (1117), Expect = 1.3e-120
Identity = 234/499 (46.89%), Postives = 325/499 (65.13%), Query Frame = 0

Query: 10  LYLLVLFILQIFTNHFLH-RIRNLPPSPYLSLPIIGQLYLLKSPLHRTLSEIA-----HR 69
           LY +   I  I +  FL  + +NLPPSP   LPIIG L LLK P+HRTL   +     + 
Sbjct: 10  LYSIFSLIFLIISFKFLKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDHND 69

Query: 70  HGPILFFRFGFRQVLVVSSPS-AAEDCLSKNDVVFANRPRLFSGKYLGYNYTSLLWAPYG 129
            G ++  R G R V VVSS   AAE+C  KNDVV ANRP++  GK++GYN T+++ APYG
Sbjct: 70  GGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAPYG 129

Query: 130 EHWRNLRRISSLEILSKHRLQTLSSIRVDEVRTLI---RRLYNAKNDVVDMRTEFFELMF 189
           +HWRNLRR+ ++EI S HRL     +R DEVR LI    RL   K  VV+++    +L F
Sbjct: 130 DHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDLTF 189

Query: 190 NIMMRMIAGKRYYGEDVAADTEEVGRFREIQEETFRLSSKTNLGDFLRVVKWVGLSKGLE 249
           N +MRM+ GKRYYGE+   D EE  R R++  +    +S  N  D++ +++   L    E
Sbjct: 190 NNIMRMMTGKRYYGEE-TTDEEEAKRVRKLVADVGANTSSGNAVDYVPILR---LFSSYE 249

Query: 250 NKLRELQMKRDVWMQSLIEEHRKQMNNTSTSLQNDAKKTMIEVLLSLQQMEPHYYKDEFI 309
           N++++L  + D ++Q LI++ R          Q +   TMI+ LL LQ+ +  YY D+ I
Sbjct: 250 NRVKKLGEETDKFLQGLIDDKRG---------QQETGTTMIDHLLVLQKSDIEYYTDQII 309

Query: 310 RGLMLVLLLAGTEGSINTMEWLLSLLLNHPDCLQRAQMEIDDVVGRSNRILEESDLAHLP 369
           +G++L++++AGT  S  T+EW LS LLNHPD + +A+ EID+ VG  +R++EE+DL+ LP
Sbjct: 310 KGIILIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVG-LDRLIEEADLSELP 369

Query: 370 YLRSLIHETLRMYPPGPLLIPHESSEDCHVGGFCVPAGTMLFVNVWAIQNDPTVWVEPGN 429
           YL++++ ETLR++P  PLL+PH +SEDC +G + +P GT L VN WAI  DP  W +P +
Sbjct: 370 YLKNIVLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDS 429

Query: 430 FNPERFGSEGEGFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFDWESVGGECVDMRE 489
           F PERF  E E  K + FG GRR CPG GL  R+VGLA+GSLIQCF+WE VG   VDM+E
Sbjct: 430 FKPERFEKEEEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKE 489

Query: 490 GGGLTLPKALPLHALCRPR 499
           G G T+PKA+PL A+C+ R
Sbjct: 490 GVGNTVPKAIPLKAICKAR 494

BLAST of Bhi09G002683 vs. ExPASy TrEMBL
Match: A0A0A0L0F1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G642290 PE=3 SV=1)

HSP 1 Score: 917.5 bits (2370), Expect = 2.4e-263
Identity = 448/507 (88.36%), Postives = 476/507 (93.89%), Query Frame = 0

Query: 5   MAIPFLYLLVLFILQIFTNHFLHRIRNLPPSPYLSLPIIGQLYLLKSPLHRTLSEIAHRH 64
           M IPFL+LL+LF+LQIFTNHFLHRIRNLPPSP+LSLPIIGQLYLLK+PLHRTLSEI+ RH
Sbjct: 1   MVIPFLHLLILFLLQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKTPLHRTLSEISRRH 60

Query: 65  GPILFFRFGFRQVLVVSSPSAAEDCLSKNDVVFANRPRLFSGKYLGYNYTSLLWAPYGEH 124
           GPI+FF+FGFR VLVVSSPSAAEDCLSKNDV+FANRPRL SGKYLGYNYTSLLWAPYGEH
Sbjct: 61  GPIVFFQFGFRSVLVVSSPSAAEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAPYGEH 120

Query: 125 WRNLRRISSLEILSKHRLQTLSSIRVDEVRTLIRRLYNAKNDVVDMRTEFFELMFNIMMR 184
           WRNLRRISSLEILS HRLQTLSSIRVDEVRTLIRRLYNA+NDVVDMRTEFFE+MFNI MR
Sbjct: 121 WRNLRRISSLEILSSHRLQTLSSIRVDEVRTLIRRLYNAENDVVDMRTEFFEVMFNITMR 180

Query: 185 MIAGKRYYGEDVAADTEEVGRFREIQEETFRLSSKTNLGDFLRVVKWVGLSKGLENKLRE 244
           MIAGKRYYGEDVAA +EE GRFREIQEETFRLS KTNLGDFLRVVKWVGLSKG+EN+LRE
Sbjct: 181 MIAGKRYYGEDVAAYSEEAGRFREIQEETFRLSGKTNLGDFLRVVKWVGLSKGIENRLRE 240

Query: 245 LQMKRDVWMQSLIEEHRKQMNN---TSTSLQNDAKKTMIEVLLSLQQMEPHYYKDEFIRG 304
           LQ+KRD WMQSLIEEHRK+MNN   +S+S+Q DAKKTMIEVLLSLQQ EP YYKDE+IRG
Sbjct: 241 LQIKRDDWMQSLIEEHRKKMNNAYSSSSSIQTDAKKTMIEVLLSLQQKEPQYYKDEYIRG 300

Query: 305 LMLVLLLAGTEGSINTMEWLLSLLLNHPDCLQRAQMEIDDVVGRSNRILEESDLAHLPYL 364
           LMLVLLLAGTEGSINTMEWLLSLLLNHP  LQRAQMEIDDVVGR+NR+LEESDL HLPYL
Sbjct: 301 LMLVLLLAGTEGSINTMEWLLSLLLNHPHSLQRAQMEIDDVVGRTNRLLEESDLTHLPYL 360

Query: 365 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFCVPAGTMLFVNVWAIQNDPTVWVEPGNFN 424
           RSLIHETLRMYPPGPLLIPHESSEDCHVGGF VPAGTMLFVNVWAIQNDPTVWVEP  FN
Sbjct: 361 RSLIHETLRMYPPGPLLIPHESSEDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVEPRKFN 420

Query: 425 PERFGSEGEGFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFDWESVGGECVDMREGG 484
           P+RFG +GEGFKWMPFGAGRRRCPGEGLGLRV+GL VGSLIQCF+WES+ GEC+DM EGG
Sbjct: 421 PDRFGGDGEGFKWMPFGAGRRRCPGEGLGLRVIGLVVGSLIQCFEWESMDGECIDMSEGG 480

Query: 485 GLTLPKALPLHALCRPRSNVAHLLPQI 509
           GLTLPKALPL  LCRPRSN  HLL QI
Sbjct: 481 GLTLPKALPLRTLCRPRSNATHLLSQI 507

BLAST of Bhi09G002683 vs. ExPASy TrEMBL
Match: A0A1S3BVF3 (cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103493585 PE=3 SV=1)

HSP 1 Score: 899.0 bits (2322), Expect = 8.8e-258
Identity = 442/512 (86.33%), Postives = 474/512 (92.58%), Query Frame = 0

Query: 1   MKMSMAIPFLYLLVLFILQIFTNHFLHRIRNLPPSPYLSLPIIGQLYLLKSPLHRTLSEI 60
           M  SM I FLYLL+LFILQIFTNHFLHRIRNLPPSP+LSLPIIGQLYLLK+PLHRTLSEI
Sbjct: 27  MVNSMVISFLYLLILFILQIFTNHFLHRIRNLPPSPFLSLPIIGQLYLLKAPLHRTLSEI 86

Query: 61  AHRHGPILFFRFGFRQVLVVSSPSAAEDCLSKNDVVFANRPRLFSGKYLGYNYTSLLWAP 120
           + RHGPILFF+FGFR VLVVSSPSA EDCLSKNDV+FANRPRL SGKYLGYNYTSLLWAP
Sbjct: 87  SRRHGPILFFQFGFRPVLVVSSPSAVEDCLSKNDVIFANRPRLVSGKYLGYNYTSLLWAP 146

Query: 121 YGEHWRNLRRISSLEILSKHRLQTLSSIRVDEVRTLIRRLYNAKNDVVDMRTEFFELMFN 180
           YGEHWRNLRRISSLEILS HRLQTLSSIRVDEVR LIRRLYNA+NDVVDMRTEFFELMFN
Sbjct: 147 YGEHWRNLRRISSLEILSSHRLQTLSSIRVDEVRKLIRRLYNAENDVVDMRTEFFELMFN 206

Query: 181 IMMRMIAGKRYYGEDVAADTEEVGRFREIQEETFRLSSKTNLGDFLRVVKWVGLSKGLEN 240
           IMMRMIAGKRYYGE V AD+EE  RF+EIQEETFRLS KTNLGDFLRVVKWVGLSKG+EN
Sbjct: 207 IMMRMIAGKRYYGEAVVADSEEGRRFQEIQEETFRLSGKTNLGDFLRVVKWVGLSKGIEN 266

Query: 241 KLRELQMKRDVWMQSLIEEHRKQMNN----TSTSLQNDAKKTMIEVLLSLQQMEPHYYKD 300
           +LRELQ++RD WMQSLIEEHRK+M+N    +S+S+Q DAKKTMIEVLLSLQQMEP  YKD
Sbjct: 267 RLRELQIRRDDWMQSLIEEHRKKMDNAHSSSSSSIQGDAKKTMIEVLLSLQQMEPQNYKD 326

Query: 301 EFIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPDCLQRAQMEIDDVVGRSNRILEESDLA 360
           E+IRGLMLVLLLAGTEGSINTMEWLLSLLLNHP  L+RAQMEID+VVG++NR+LEESDL 
Sbjct: 327 EYIRGLMLVLLLAGTEGSINTMEWLLSLLLNHPHSLRRAQMEIDNVVGKTNRLLEESDLT 386

Query: 361 HLPYLRSLIHETLRMYPPGPLLIPHESSEDCHVGGFCVPAGTMLFVNVWAIQNDPTVWVE 420
           HLPYLRSLIHETLRMYPPGPLLIPHESS+DCHVGGF VPAGTMLFVNVWAIQNDPTVWVE
Sbjct: 387 HLPYLRSLIHETLRMYPPGPLLIPHESSQDCHVGGFHVPAGTMLFVNVWAIQNDPTVWVE 446

Query: 421 PGNFNPERFGSEGEGFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFDWESVGGECVD 480
           P  FNP+RF S+GEGFKWMPFGAGRRRCPGE LGLRVVGL +GSLIQCF+WES+GG+C+D
Sbjct: 447 PEKFNPDRFKSDGEGFKWMPFGAGRRRCPGESLGLRVVGLVIGSLIQCFEWESMGGKCID 506

Query: 481 MREGGGLTLPKALPLHALCRPRSNVAHLLPQI 509
           M EGGGLTLPKALPL  LCRPRSN  HLL QI
Sbjct: 507 MSEGGGLTLPKALPLRTLCRPRSNATHLLSQI 538

BLAST of Bhi09G002683 vs. ExPASy TrEMBL
Match: A0A6J1FED9 (isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111443311 PE=3 SV=1)

HSP 1 Score: 889.8 bits (2298), Expect = 5.3e-255
Identity = 444/506 (87.75%), Postives = 464/506 (91.70%), Query Frame = 0

Query: 5   MAIPFLYLLVLFILQIFTNHFLHRIRNLPPSPYLSLPIIGQLYLLKSPLHRTLSEIAHRH 64
           MAI FL LL+L IL IFTN FLHRIRNLPPSP+ SLPIIGQLYLLK PLHRTLS+I+ RH
Sbjct: 1   MAIIFLCLLILLILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLSDISRRH 60

Query: 65  GPILFFRFGFRQVLVVSSPSAAEDCLSKNDVVFANRPRLFSGKYLGYNYTSLLWAPYGEH 124
           GPILFF FGFR VLVVSSPSAAE+CLSKND+VFANRP L  GKYLGYNYTSLLWAPYGEH
Sbjct: 61  GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120

Query: 125 WRNLRRISSLEILSKHRLQTLSSIRVDEVRTLIRRLYNAKNDVVDMRTEFFELMFNIMMR 184
           WRNLRRISSLEI S HRLQTLSSIRVDEVRTLIRRLYN KNDVVDMRTEFFELMFNIMMR
Sbjct: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMRTEFFELMFNIMMR 180

Query: 185 MIAGKRYYGEDVAADTEEVGRFREIQEETFRLSSKTNLGDFLRVVKWVGLSKGLENKLRE 244
           MIAGKRYYGED AA++EE  RFREIQEETFRLSSKTNLGDFLR+VKW GLSKGLEN+++E
Sbjct: 181 MIAGKRYYGED-AAESEEAERFREIQEETFRLSSKTNLGDFLRIVKWAGLSKGLENRMKE 240

Query: 245 LQMKRDVWMQSLIEEHRKQMNNTSTSLQNDAKKTMIEVLLSLQQMEPHYYKDEFIRGLML 304
           LQ KRD WMQSLIEEHRKQ        + DAKKTMIEVLLSLQQ EPHYYKDE+IRGLML
Sbjct: 241 LQSKRDAWMQSLIEEHRKQS-------KKDAKKTMIEVLLSLQQKEPHYYKDEYIRGLML 300

Query: 305 VLLLAGTEGSINTMEWLLSLLLNHPDCLQRAQMEIDDVVGRSNRILEESDLAHLPYLRSL 364
           VLLLAGTEGSINTMEWLLSLLLN+P+CLQRAQ EIDDVVGRSNRILEESDLAHLPYLRSL
Sbjct: 301 VLLLAGTEGSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYLRSL 360

Query: 365 IHETLRMYPPGPLLIPHESSEDCHVGGFCVPAGTMLFVNVWAIQNDPTVWVEPGNFNPER 424
           IHETLRMYPPGPLLIPHESSEDCHVGGF VPAGT LFVNVWAIQNDPTVWV+P  FNP R
Sbjct: 361 IHETLRMYPPGPLLIPHESSEDCHVGGFRVPAGTTLFVNVWAIQNDPTVWVDPEKFNPGR 420

Query: 425 FGSEGEG--FKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFDWESVGGECVDMREGGG 484
           FGSEGEG  FKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCF+WESVGGEC+DMREG G
Sbjct: 421 FGSEGEGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMREGVG 480

Query: 485 LTLPKALPLHALCRPRSNVAHLLPQI 509
           LTLPKA+PLHALCRPRS VAHLLPQI
Sbjct: 481 LTLPKAVPLHALCRPRSKVAHLLPQI 498

BLAST of Bhi09G002683 vs. ExPASy TrEMBL
Match: A0A6J1INS3 (isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111477207 PE=3 SV=1)

HSP 1 Score: 886.3 bits (2289), Expect = 5.9e-254
Identity = 442/506 (87.35%), Postives = 463/506 (91.50%), Query Frame = 0

Query: 5   MAIPFLYLLVLFILQIFTNHFLHRIRNLPPSPYLSLPIIGQLYLLKSPLHRTLSEIAHRH 64
           MAI FL LL+LFIL IFTN FLHRIRNLPPSP+ SLPIIGQLYLLK PLHRTL +I+ RH
Sbjct: 1   MAITFLCLLILFILHIFTNQFLHRIRNLPPSPFFSLPIIGQLYLLKRPLHRTLLDISRRH 60

Query: 65  GPILFFRFGFRQVLVVSSPSAAEDCLSKNDVVFANRPRLFSGKYLGYNYTSLLWAPYGEH 124
           GPILFF FGFR VLVVSSPSAAE+CLSKND+VFANRP L  GKYLGYNYTSLLWAPYGEH
Sbjct: 61  GPILFFWFGFRPVLVVSSPSAAEECLSKNDIVFANRPHLIGGKYLGYNYTSLLWAPYGEH 120

Query: 125 WRNLRRISSLEILSKHRLQTLSSIRVDEVRTLIRRLYNAKNDVVDMRTEFFELMFNIMMR 184
           WRNLRRISSLEI S HRLQTLSSIRVDEVRTLIRRLYN KNDVVDM+TEFFELMFNIMMR
Sbjct: 121 WRNLRRISSLEIFSTHRLQTLSSIRVDEVRTLIRRLYNTKNDVVDMKTEFFELMFNIMMR 180

Query: 185 MIAGKRYYGEDVAADTEEVGRFREIQEETFRLSSKTNLGDFLRVVKWVGLSKGLENKLRE 244
           MIAGKRYYGEDV A++EE  RFREIQEETFRLSSKTNLGDFLR+VKW GLSKGLEN+++E
Sbjct: 181 MIAGKRYYGEDV-AESEEAERFREIQEETFRLSSKTNLGDFLRMVKWAGLSKGLENRMKE 240

Query: 245 LQMKRDVWMQSLIEEHRKQMNNTSTSLQNDAKKTMIEVLLSLQQMEPHYYKDEFIRGLML 304
           LQ KRD WMQSLIEEHRKQ        + DAKKTMIEVLLSLQQ EPHYYKDE+IRGLML
Sbjct: 241 LQSKRDAWMQSLIEEHRKQS-------KKDAKKTMIEVLLSLQQKEPHYYKDEYIRGLML 300

Query: 305 VLLLAGTEGSINTMEWLLSLLLNHPDCLQRAQMEIDDVVGRSNRILEESDLAHLPYLRSL 364
           VLLLAGTEGSINTMEWLLSLLLN+P+CLQRAQ EIDDVVGRSNRILEESDLAHLPYLRSL
Sbjct: 301 VLLLAGTEGSINTMEWLLSLLLNNPECLQRAQTEIDDVVGRSNRILEESDLAHLPYLRSL 360

Query: 365 IHETLRMYPPGPLLIPHESSEDCHVGGFCVPAGTMLFVNVWAIQNDPTVWVEPGNFNPER 424
           IHETLRMYPPGPLLIPHESS DCHVGGF VPAGT LFVNVWAIQNDPTVWV+P  FNP R
Sbjct: 361 IHETLRMYPPGPLLIPHESSADCHVGGFRVPAGTTLFVNVWAIQNDPTVWVDPEKFNPGR 420

Query: 425 FGSEGEG--FKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFDWESVGGECVDMREGGG 484
           FGSEGEG  FKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCF+WESVGGEC+DMREG G
Sbjct: 421 FGSEGEGWFFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFEWESVGGECIDMREGVG 480

Query: 485 LTLPKALPLHALCRPRSNVAHLLPQI 509
           LTLPKA+PLHALCRPRS VAHLLPQI
Sbjct: 481 LTLPKAVPLHALCRPRSKVAHLLPQI 498

BLAST of Bhi09G002683 vs. ExPASy TrEMBL
Match: A0A6P4BCT8 (cytochrome P450 81E8-like OS=Ziziphus jujuba OX=326968 GN=LOC107433678 PE=3 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 7.9e-182
Identity = 324/514 (63.04%), Postives = 399/514 (77.63%), Query Frame = 0

Query: 9   FLYLLVLFILQIFTNHFLHRIRNLPPSPYLSLPIIGQLYLLKSPLHRTLSEIAHRHGPIL 68
           F Y+L+   + +FT H  H+I+NLPPSP+  LP+IG LYLL+ PLHRTLS++A R+GPIL
Sbjct: 6   FFYILLFSAVYVFTKHLFHKIQNLPPSPF-PLPVIGHLYLLERPLHRTLSKLAGRYGPIL 65

Query: 69  FFRFGFRQVLVVSSPSAAEDCLSKNDVVFANRPRLFSGKYLGYNYTSLLWAPYGEHWRNL 128
           F + G R VLVVSS SAAE+CL+KND++FANRPRL  GKYLGYN+TS+ WAPYG+HWRNL
Sbjct: 66  FLKLGSRPVLVVSSSSAAEECLAKNDIIFANRPRLMVGKYLGYNFTSVAWAPYGDHWRNL 125

Query: 129 RRISSLEILSKHRLQTLSSIRVDEVRTLIRRLYNAKNDVVDMRTEFFELMFNIMMRMIAG 188
           RRISSLE+LS HRLQ LS IR++EV+ L+RRL+  +  VVDMR  FFEL  NIMMRMIAG
Sbjct: 126 RRISSLELLSSHRLQMLSGIRLEEVKLLLRRLFEKQGVVVDMRQAFFELTLNIMMRMIAG 185

Query: 189 KRYYGEDVAADTEEVGRFREIQEETFRLSSKTNLGDFLRVVKWVGLSKGLENKLRELQMK 248
           KRYYG++V  D +E  RFREIQEET RLS K+NLGDFL  V+W+G SK LE  L ELQ K
Sbjct: 186 KRYYGDEVVEDLKEAMRFREIQEETVRLSGKSNLGDFLPFVRWIGGSKRLEKSLMELQRK 245

Query: 249 RDVWMQSLIEEHRKQMN---------NTSTSLQNDAKKTMIEVLLSLQQMEPHYYKDEFI 308
           RD ++Q LIEEHR+  +         + S S   + +KTM+EVLLSLQQMEP YYKDE I
Sbjct: 246 RDGFLQDLIEEHRRTASRIANDCCSPSPSPSKSKERRKTMLEVLLSLQQMEPEYYKDEII 305

Query: 309 RGLMLVLLLAGTEGSINTMEWLLSLLLNHPDCLQRAQMEIDDVVGRSNRILEESDLAHLP 368
           RGLMLVLLL GT+ +INT++W LSLLLNHP+ L++AQ EID+ VG   R+++ESD+A LP
Sbjct: 306 RGLMLVLLLGGTDTTINTLQWSLSLLLNHPEILKKAQDEIDNRVGH-GRLVDESDVAQLP 365

Query: 369 YLRSLIHETLRMYPPGPLLIPHESSEDCHVGGFCVPAGTMLFVNVWAIQNDPTVWVEPGN 428
           YL+S+I+ETLRMYPP PLL+PHESSE+  VGGF +P GT L VN+WAIQNDPT+WV+P +
Sbjct: 366 YLKSIINETLRMYPPIPLLLPHESSEESTVGGFRIPRGTTLLVNIWAIQNDPTIWVDPTS 425

Query: 429 FNPERF----GSEGEGFKWMPFGAGRRRCPGEGLGLRVVGLAVGSLIQCFDWESVGGE-C 488
           F PER+    G   +  + MPFG+GRR CPGEGLG+RVVGL +G +IQCF+WE V  E  
Sbjct: 426 FKPERYIEAEGMRNDKLRLMPFGSGRRGCPGEGLGMRVVGLVLGLIIQCFNWERVSQEKM 485

Query: 489 VDMREGGGLTLPKALPLHALCRPRSNVAHLLPQI 509
           VDM EG GLTLPK+ PL A C PRS ++ L+ QI
Sbjct: 486 VDMSEGNGLTLPKSQPLQAKCWPRSTMSKLVSQI 517