Bhi09G002679 (gene) Wax gourd (B227) v1

Overview
NameBhi09G002679
Typegene
OrganismBenincasa hispida (Wax gourd (B227) v1)
Description3-oxo-Delta(4,5)-steroid 5-beta-reductase
Locationchr9: 83325422 .. 83327193 (-)
RNA-Seq ExpressionBhi09G002679
SyntenyBhi09G002679
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTATCCGTTCACAGCTCGCGTCACGGTTCGCGTTTACCTTGTGATCAGACAATCTTACGCTTATAACCCTTTCAACCACCGCATCCATTTCCAATTCATTCTCTGTTTCTTGTCTTCTCCCTTTTCCCTAATTCCTTTACCTAGCTCCGATTTCGCTACAGAAAATGAGCTGGTGGTGGGCCGGAGCAATCGGCGCTGCTAAGGTAAACTCTATAACCCCACTATCTCTCTATCCGTTCCCCCCCCCCCCTTTCTACTCTGAGATGTATCCACGTTTCTTTAATCCACTTCTCTGTTCCCAATCTTGCAGAAGAAGTTTGAAGAAGATGAACCTCCGAGGAGCTTTGAGAGCGTAGCCCTGGTGATCGGCGTTACTGGTATCGTCGGCAACAGCCTCGCCGAGATTCTCCCTCTTTCCGATACCCCCGGCGGTCCATGGAAGGTCTACGGCATCGCTCGGAGGCCGAGGCCAACCTGGAATGCCGACCATCCCATCGAATACATTCAATGCGATATCTCCGATCCAGAAGATGCCGAAGCCAAACTCTCGCAGCTCACTGATGTGACTCATCTCTTCTATGTCACATGGACCAATCGACCCACCGAGATCGAGAATTGCGAGGCAAACGTTAAAATGCTTCGCAACGTTTTGCGATCAGTGATCCCCAACGCACCGAATCTCCGCCATATCTGCCTTCAAACCGGTACGAAGCACTACCTCGGATCGCTCCAGAGCATTGTCGATAAATCGGTGCAGCCGCACGATCCGCCTTTCACGGAGGACTTGCCTCGATTGGAAGGCCCAAATTTCTATTACTCACAAGAAGATTTGTTATTCGAAGAGATTGAACAGTCTCAGAAGAAGGATCTGACATGGGCGGTGATCAGACCCAATGTAATTTTCGGGTTTTCCCCTTTTAGTTTGATAAACATCGTCGGAACCCTATGCGTCTACGCCGCCATATGCAAGCACGAGGGACGTACGTTGAAATTCCCCGGCTCCAAATCGGCTTGGGAGGATTTCCAGGTGGGGTCCGACGCAGATCTGATAGCGGAGCAGCACATATGGACGGCGGTTGATCCGTACGCGAAGAACGAGGCGTTTAACTGCAACAATGGCGATGTTTTCAAGTGGAAGCACTTCTGGAAGGTGTTGGCAGAGCAGTTTAACATAGAAGATTATGGATTCGAAGATGGGGAGAGATTGACGTTGGTGGAGTTGATGAAGGACAAAAGTGAAGTGTGGGATGAGATTGTGAAGGAGAATCAATTGCAACCAACCAAATTGGAGGAGATTACTAATTGGTGGTTTACAGATGTAATTTTCAGCCTCTCAGGTAACATAGATAGTATGAACAAGAGCAAAGAACATGGGTTTTTGGGCTTCAGGAACTCTAAGAACTCCTTCATTTCGTGGATTGACAAGATTAAGGCTTTCAAAATTGTGCCTTGATTGGCCTTGGAACTGTTCGTTTGGATTTTTTATCCTGCTAGTCTGATCCTTTTTCATGGTTTTGCAATAAGCTTGATCATAGAAAGTGTTGCTTTATTTACTTTCTTGGTATCTTTAAAGTGATTGAGTTATTTGTGATTGGAGTCTATAAAAATAATGGAAAATTCAACGAGTATTATCAATAGCATTCTAGAAAGTAATAGTGCTTTATAATATTCAAATGAGATTGCTTTAAAGTGATCGAGTTATTTGCAATAAGCTTGATCATAGAAAGTGTTGCTTTATTTACTTTCTTGGTAAAACAGTCTCAGTGTCGCAG

mRNA sequence

TTATCCGTTCACAGCTCGCGTCACGGTTCGCGTTTACCTTGTGATCAGACAATCTTACGCTTATAACCCTTTCAACCACCGCATCCATTTCCAATTCATTCTCTGTTTCTTGTCTTCTCCCTTTTCCCTAATTCCTTTACCTAGCTCCGATTTCGCTACAGAAAATGAGCTGGTGGTGGGCCGGAGCAATCGGCGCTGCTAAGAAGAAGTTTGAAGAAGATGAACCTCCGAGGAGCTTTGAGAGCGTAGCCCTGGTGATCGGCGTTACTGGTATCGTCGGCAACAGCCTCGCCGAGATTCTCCCTCTTTCCGATACCCCCGGCGGTCCATGGAAGGTCTACGGCATCGCTCGGAGGCCGAGGCCAACCTGGAATGCCGACCATCCCATCGAATACATTCAATGCGATATCTCCGATCCAGAAGATGCCGAAGCCAAACTCTCGCAGCTCACTGATGTGACTCATCTCTTCTATGTCACATGGACCAATCGACCCACCGAGATCGAGAATTGCGAGGCAAACGTTAAAATGCTTCGCAACGTTTTGCGATCAGTGATCCCCAACGCACCGAATCTCCGCCATATCTGCCTTCAAACCGGTACGAAGCACTACCTCGGATCGCTCCAGAGCATTGTCGATAAATCGGTGCAGCCGCACGATCCGCCTTTCACGGAGGACTTGCCTCGATTGGAAGGCCCAAATTTCTATTACTCACAAGAAGATTTGTTATTCGAAGAGATTGAACAGTCTCAGAAGAAGGATCTGACATGGGCGGTGATCAGACCCAATGTAATTTTCGGGTTTTCCCCTTTTAGTTTGATAAACATCGTCGGAACCCTATGCGTCTACGCCGCCATATGCAAGCACGAGGGACGTACGTTGAAATTCCCCGGCTCCAAATCGGCTTGGGAGGATTTCCAGGTGGGGTCCGACGCAGATCTGATAGCGGAGCAGCACATATGGACGGCGGTTGATCCGTACGCGAAGAACGAGGCGTTTAACTGCAACAATGGCGATGTTTTCAAGTGGAAGCACTTCTGGAAGGTGTTGGCAGAGCAGTTTAACATAGAAGATTATGGATTCGAAGATGGGGAGAGATTGACGTTGGTGGAGTTGATGAAGGACAAAAGTGAAGTGTGGGATGAGATTGTGAAGGAGAATCAATTGCAACCAACCAAATTGGAGGAGATTACTAATTGGTGGTTTACAGATGTAATTTTCAGCCTCTCAGGTAACATAGATAGTATGAACAAGAGCAAAGAACATGGGTTTTTGGGCTTCAGGAACTCTAAGAACTCCTTCATTTCGTGGATTGACAAGATTAAGGCTTTCAAAATTGTGCCTTGATTGGCCTTGGAACTGTTCGTTTGGATTTTTTATCCTGCTAGTCTGATCCTTTTTCATGGTTTTGCAATAAGCTTGATCATAGAAAGTGTTGCTTTATTTACTTTCTTGGTATCTTTAAAGTGATTGAGTTATTTGTGATTGGAGTCTATAAAAATAATGGAAAATTCAACGAGTATTATCAATAGCATTCTAGAAAGTAATAGTGCTTTATAATATTCAAATGAGATTGCTTTAAAGTGATCGAGTTATTTGCAATAAGCTTGATCATAGAAAGTGTTGCTTTATTTACTTTCTTGGTAAAACAGTCTCAGTGTCGCAG

Coding sequence (CDS)

ATGAGCTGGTGGTGGGCCGGAGCAATCGGCGCTGCTAAGAAGAAGTTTGAAGAAGATGAACCTCCGAGGAGCTTTGAGAGCGTAGCCCTGGTGATCGGCGTTACTGGTATCGTCGGCAACAGCCTCGCCGAGATTCTCCCTCTTTCCGATACCCCCGGCGGTCCATGGAAGGTCTACGGCATCGCTCGGAGGCCGAGGCCAACCTGGAATGCCGACCATCCCATCGAATACATTCAATGCGATATCTCCGATCCAGAAGATGCCGAAGCCAAACTCTCGCAGCTCACTGATGTGACTCATCTCTTCTATGTCACATGGACCAATCGACCCACCGAGATCGAGAATTGCGAGGCAAACGTTAAAATGCTTCGCAACGTTTTGCGATCAGTGATCCCCAACGCACCGAATCTCCGCCATATCTGCCTTCAAACCGGTACGAAGCACTACCTCGGATCGCTCCAGAGCATTGTCGATAAATCGGTGCAGCCGCACGATCCGCCTTTCACGGAGGACTTGCCTCGATTGGAAGGCCCAAATTTCTATTACTCACAAGAAGATTTGTTATTCGAAGAGATTGAACAGTCTCAGAAGAAGGATCTGACATGGGCGGTGATCAGACCCAATGTAATTTTCGGGTTTTCCCCTTTTAGTTTGATAAACATCGTCGGAACCCTATGCGTCTACGCCGCCATATGCAAGCACGAGGGACGTACGTTGAAATTCCCCGGCTCCAAATCGGCTTGGGAGGATTTCCAGGTGGGGTCCGACGCAGATCTGATAGCGGAGCAGCACATATGGACGGCGGTTGATCCGTACGCGAAGAACGAGGCGTTTAACTGCAACAATGGCGATGTTTTCAAGTGGAAGCACTTCTGGAAGGTGTTGGCAGAGCAGTTTAACATAGAAGATTATGGATTCGAAGATGGGGAGAGATTGACGTTGGTGGAGTTGATGAAGGACAAAAGTGAAGTGTGGGATGAGATTGTGAAGGAGAATCAATTGCAACCAACCAAATTGGAGGAGATTACTAATTGGTGGTTTACAGATGTAATTTTCAGCCTCTCAGGTAACATAGATAGTATGAACAAGAGCAAAGAACATGGGTTTTTGGGCTTCAGGAACTCTAAGAACTCCTTCATTTCGTGGATTGACAAGATTAAGGCTTTCAAAATTGTGCCTTGA

Protein sequence

MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYGIARRPRPTWNADHPIEYIQCDISDPEDAEAKLSQLTDVTHLFYVTWTNRPTEIENCEANVKMLRNVLRSVIPNAPNLRHICLQTGTKHYLGSLQSIVDKSVQPHDPPFTEDLPRLEGPNFYYSQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLINIVGTLCVYAAICKHEGRTLKFPGSKSAWEDFQVGSDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFNIEDYGFEDGERLTLVELMKDKSEVWDEIVKENQLQPTKLEEITNWWFTDVIFSLSGNIDSMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP
Homology
[Close]
BLAST of Bhi09G002679 vs. TAIR 10
Match: AT4G24220.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 646.0 bits (1665), Expect = 2.0e-185
Identity = 297/393 (75.57%), Postives = 336/393 (85.50%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKK +EDEP +SFESVAL+IGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKLDEDEPSQSFESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  IARRPRPTWNADHPIEYIQCDISDPEDAEAKLSQLTDVTHLFYVTWTNRPTEIENCEANV 120
           +ARRPRPTWNADHPI+YIQCD+SD ED  +KLS LTDVTH+FYVTWTNR +E ENCEAN 
Sbjct: 61  VARRPRPTWNADHPIDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNRESESENCEANG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYLGSLQSIVDKSVQPHDPPFTEDLPRLEGPNF 180
            MLRNVL+++IP APNLRH+CLQTGTKHYLG   ++       HDPPFTED+PRL+  NF
Sbjct: 121 SMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPFTNV---DGPRHDPPFTEDMPRLQIQNF 180

Query: 181 YYSQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLINIVGTLCVYAAICKHEGRTLK 240
           YY+QED+LFEEI++ +   +TW++ RPN+IFGFSP+SL+NIVGTLCVYAAICKHEG  L 
Sbjct: 181 YYTQEDILFEEIKKIE--TVTWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLL 240

Query: 241 FPGSKSAWEDFQVGSDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPGSK AWE F   SDADLIAEQ IW AVDPYAKNEAFNCNN D+FKWKH WK+LAEQF 
Sbjct: 241 FPGSKKAWEGFMTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKILAEQFG 300

Query: 301 IEDYGFEDGERLTLVELMKDKSEVWDEIVKENQLQPTKLEEITNWWFTDVIFSLSGNIDS 360
           IE+YGFE+G+ L LVE+MK K  VW+E+VKENQLQ  KLEE+  WWF DVI  + G IDS
Sbjct: 301 IEEYGFEEGKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEGMIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 394
           MNKSKE+GFLGFRNS NSFISWIDK KAFKIVP
Sbjct: 361 MNKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP 388

[Close]
BLAST of Bhi09G002679 vs. TAIR 10
Match: AT4G24220.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 639.4 bits (1648), Expect = 1.9e-183
Identity = 296/393 (75.32%), Postives = 335/393 (85.24%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAA KK +EDEP +SFESVAL+IGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAA-KKLDEDEPSQSFESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  IARRPRPTWNADHPIEYIQCDISDPEDAEAKLSQLTDVTHLFYVTWTNRPTEIENCEANV 120
           +ARRPRPTWNADHPI+YIQCD+SD ED  +KLS LTDVTH+FYVTWTNR +E ENCEAN 
Sbjct: 61  VARRPRPTWNADHPIDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNRESESENCEANG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYLGSLQSIVDKSVQPHDPPFTEDLPRLEGPNF 180
            MLRNVL+++IP APNLRH+CLQTGTKHYLG   ++       HDPPFTED+PRL+  NF
Sbjct: 121 SMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPFTNV---DGPRHDPPFTEDMPRLQIQNF 180

Query: 181 YYSQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLINIVGTLCVYAAICKHEGRTLK 240
           YY+QED+LFEEI++ +   +TW++ RPN+IFGFSP+SL+NIVGTLCVYAAICKHEG  L 
Sbjct: 181 YYTQEDILFEEIKKIE--TVTWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLL 240

Query: 241 FPGSKSAWEDFQVGSDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPGSK AWE F   SDADLIAEQ IW AVDPYAKNEAFNCNN D+FKWKH WK+LAEQF 
Sbjct: 241 FPGSKKAWEGFMTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKILAEQFG 300

Query: 301 IEDYGFEDGERLTLVELMKDKSEVWDEIVKENQLQPTKLEEITNWWFTDVIFSLSGNIDS 360
           IE+YGFE+G+ L LVE+MK K  VW+E+VKENQLQ  KLEE+  WWF DVI  + G IDS
Sbjct: 301 IEEYGFEEGKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEGMIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 394
           MNKSKE+GFLGFRNS NSFISWIDK KAFKIVP
Sbjct: 361 MNKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP 387

[Close]
BLAST of Bhi09G002679 vs. TAIR 10
Match: AT5G58750.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 236.1 bits (601), Expect = 4.8e-62
Identity = 137/383 (35.77%), Postives = 211/383 (55.09%), Query Frame = 0 

Query: 26  ESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYGIARRPRPTWNADHPIEYIQCDISDP 85
           E+VAL+ GVTG+VG  + + L L   PG  W++YG+AR P    +      +I CD+ + 
Sbjct: 16  ENVALIFGVTGLVGREIVKTL-LMSKPG--WRIYGVARNPEIN-SMTKMYNFISCDLLNA 75

Query: 86  EDAEAKLSQLTD-VTHLFYVTWTNR-PTEI-ENCEANVKMLRNVLRSVIPNAPNLRHICL 145
            + + +LS L D V+H+F+VTW+   P +  E C  N  ML N L +++PNA  L+H  L
Sbjct: 76  SETKQRLSPLQDIVSHVFWVTWSGEFPLDTDECCVQNKTMLMNALDAILPNAKRLKHFSL 135

Query: 146 QTGTKHYLGSLQSIVDKSVQPHDPPFTEDLPR-LEGPNFYYSQEDLLFEEIEQSQKKDLT 205
           QTG KHY+  ++  + +        ++E+ PR   G NFYY  EDLL E+I +S    + 
Sbjct: 136 QTGMKHYVSLVEETMARGEGSSLYYYSEECPRKSSGKNFYYVLEDLLKEKITRS---SVV 195

Query: 206 WAVIRPNVIFGFSPFSLINIVGTLCVYAAICKHEGRTLKFPGSKSAWEDFQV-GSDADLI 265
           W+V RP ++ G S  +L N +G+LCVY A+CK+      F G++  WE+  + GSD++L+
Sbjct: 196 WSVQRPGLLMGSSSRTLYNFMGSLCVYGAMCKYLNLPFVFGGTRECWEESYIDGSDSNLV 255

Query: 266 AEQHIWTAVDPYA--KNEAFNCNNGDVFKWKHFWKVLAEQFNIE-------DYGFEDGER 325
           AEQHI+ A       K EAFN  NG  F WK  W  + ++  ++       D GF  G  
Sbjct: 256 AEQHIFAATSGKVREKGEAFNAINGVGFTWKEIWPEIGKKLGVQVNETTMFDEGFWFGRE 315

Query: 326 LTLVELMKDKSEVWDEIVKENQLQPTKLEEITNWWFTDVIFSLSGN-IDSMNKSKEHGFL 385
                 M ++  VWDEIV + +L  T++E++ NW+F D +F      +    K    GF 
Sbjct: 316 ------MVERKHVWDEIVVKEKLVRTEIEDLANWYFLDALFRCPFKLLGKREKVDRFGFK 375

Query: 386 GFRNSKNSFISWIDKIKAFKIVP 394
               + +S + WID ++  K++P
Sbjct: 376 RKYRTLDSVLYWIDVMRDEKLIP 385

[Close]
BLAST of Bhi09G002679 vs. ExPASy Swiss-Prot
Match: Q9STX2 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP1 PE=1 SV=1)

HSP 1 Score: 646.0 bits (1665), Expect = 2.8e-184
Identity = 297/393 (75.57%), Postives = 336/393 (85.50%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKK +EDEP +SFESVAL+IGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKLDEDEPSQSFESVALIIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  IARRPRPTWNADHPIEYIQCDISDPEDAEAKLSQLTDVTHLFYVTWTNRPTEIENCEANV 120
           +ARRPRPTWNADHPI+YIQCD+SD ED  +KLS LTDVTH+FYVTWTNR +E ENCEAN 
Sbjct: 61  VARRPRPTWNADHPIDYIQCDVSDAEDTRSKLSPLTDVTHVFYVTWTNRESESENCEANG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYLGSLQSIVDKSVQPHDPPFTEDLPRLEGPNF 180
            MLRNVL+++IP APNLRH+CLQTGTKHYLG   ++       HDPPFTED+PRL+  NF
Sbjct: 121 SMLRNVLQAIIPYAPNLRHVCLQTGTKHYLGPFTNV---DGPRHDPPFTEDMPRLQIQNF 180

Query: 181 YYSQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLINIVGTLCVYAAICKHEGRTLK 240
           YY+QED+LFEEI++ +   +TW++ RPN+IFGFSP+SL+NIVGTLCVYAAICKHEG  L 
Sbjct: 181 YYTQEDILFEEIKKIE--TVTWSIHRPNMIFGFSPYSLMNIVGTLCVYAAICKHEGSPLL 240

Query: 241 FPGSKSAWEDFQVGSDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPGSK AWE F   SDADLIAEQ IW AVDPYAKNEAFNCNN D+FKWKH WK+LAEQF 
Sbjct: 241 FPGSKKAWEGFMTASDADLIAEQQIWAAVDPYAKNEAFNCNNADIFKWKHLWKILAEQFG 300

Query: 301 IEDYGFEDGERLTLVELMKDKSEVWDEIVKENQLQPTKLEEITNWWFTDVIFSLSGNIDS 360
           IE+YGFE+G+ L LVE+MK K  VW+E+VKENQLQ  KLEE+  WWF DVI  + G IDS
Sbjct: 301 IEEYGFEEGKNLGLVEMMKGKERVWEEMVKENQLQEKKLEEVGVWWFADVILGVEGMIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 394
           MNKSKE+GFLGFRNS NSFISWIDK KAFKIVP
Sbjct: 361 MNKSKEYGFLGFRNSNNSFISWIDKYKAFKIVP 388

[Close]
BLAST of Bhi09G002679 vs. ExPASy Swiss-Prot
Match: A0A221J5W8 ((S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta cataria OX=39347 GN=ISY1 PE=1 SV=1)

HSP 1 Score: 614.8 bits (1584), Expect = 6.9e-175
Identity = 278/393 (70.74%), Postives = 328/393 (83.46%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKK +EDE PR++ESVAL++GVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKIDEDEAPRNYESVALIVGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  IARRPRPTWNADHPIEYIQCDISDPEDAEAKLSQLTDVTHLFYVTWTNRPTEIENCEANV 120
           +ARRPRP+WN DHPI YI CD+ D  D EAKLS LTDVTH+FY TWT R TE ENCEAN 
Sbjct: 61  VARRPRPSWNEDHPITYISCDVLDSVDVEAKLSPLTDVTHIFYATWTKRSTEKENCEANG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYLGSLQSIVDKSVQPHDPPFTEDLPRLEGPNF 180
           KML+NVL ++IPN PNL+HICLQTG KHYLG+ + +  K    HDPP TEDLPRL+  N+
Sbjct: 121 KMLKNVLNAMIPNCPNLKHICLQTGRKHYLGAFEDLKSKC---HDPPLTEDLPRLDSQNY 180

Query: 181 YYSQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLINIVGTLCVYAAICKHEGRTLK 240
           YY+QED+LFEE++  +K+ LTW+V RP  IFGFSP+S++N+VGTLCVYAAICKHEG  L+
Sbjct: 181 YYTQEDILFEEVQ--KKEGLTWSVHRPGTIFGFSPYSMMNLVGTLCVYAAICKHEGAVLR 240

Query: 241 FPGSKSAWEDFQVGSDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPG K AW+ +   SDADLIAE  IW AVDPYAKNEAFN +NGDVFKWKHFWKVLAEQF 
Sbjct: 241 FPGCKGAWDGYSDCSDADLIAEHQIWAAVDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFG 300

Query: 301 IEDYGFEDGERLTLVELMKDKSEVWDEIVKENQLQPTKLEEITNWWFTDVIFSLSGNIDS 360
           +E   +E+G+++ L +LMKDK  VWD+IV+EN L  TKLE++ NWWF+D++      +D+
Sbjct: 301 VECGEYEEGQQVKLQDLMKDKGPVWDKIVRENGLSTTKLEDVGNWWFSDIVLGNECWLDT 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 394
           MNKSKEHGFLGFRNSKNSFISWIDK+KAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 388

[Close]
BLAST of Bhi09G002679 vs. ExPASy Swiss-Prot
Match: A0A221J5X3 ((S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta racemosa OX=54731 GN=ISY1 PE=1 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 7.7e-174
Identity = 277/393 (70.48%), Postives = 327/393 (83.21%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKK+ +EDE PR++ESVAL++GVTGIVGNSLAEILPLSDTP GPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKRIDEDEAPRNYESVALIVGVTGIVGNSLAEILPLSDTPCGPWKVYG 60

Query: 61  IARRPRPTWNADHPIEYIQCDISDPEDAEAKLSQLTDVTHLFYVTWTNRPTEIENCEANV 120
           +ARRPRP+WN DHPI YI CD+ D  D EAKLS LTDVTH+FY TWT R TE ENCEAN 
Sbjct: 61  VARRPRPSWNEDHPITYISCDVLDSVDVEAKLSPLTDVTHIFYATWTKRSTEKENCEANG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYLGSLQSIVDKSVQPHDPPFTEDLPRLEGPNF 180
           KML+NVL ++IPN PNL+HICLQTG KHYLG+ +   D  ++ HDPP TEDLPRL+  NF
Sbjct: 121 KMLKNVLNAMIPNCPNLKHICLQTGRKHYLGAFE---DWKIKRHDPPLTEDLPRLDSQNF 180

Query: 181 YYSQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLINIVGTLCVYAAICKHEGRTLK 240
           YY+QED+LFEE++  +K+ LTW+V RP  IFGFSP+S++N+VGTLCVYAAICKHEG  L+
Sbjct: 181 YYTQEDILFEEVQ--KKESLTWSVHRPGTIFGFSPYSMMNLVGTLCVYAAICKHEGAVLR 240

Query: 241 FPGSKSAWEDFQVGSDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPG K AW+ +   SDADLIAE  IW AVDPYAKNEAFN +NGDVFKWKHFWKVLAEQF 
Sbjct: 241 FPGCKGAWDGYSDCSDADLIAEHQIWAAVDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFG 300

Query: 301 IEDYGFEDGERLTLVELMKDKSEVWDEIVKENQLQPTKLEEITNWWFTDVIFSLSGNIDS 360
           +E   +E+G+ + L +LMKDK  VWD+IV+EN L  TKLE++ NWWF+D++      +D+
Sbjct: 301 VECGEYEEGKEVKLQDLMKDKGPVWDKIVRENGLSTTKLEDVGNWWFSDIVLGNECWLDT 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 394
           MNKSKEHGFLGFRNSKNSFISWIDK+KAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKVKAFKIVP 388

[Close]
BLAST of Bhi09G002679 vs. ExPASy Swiss-Prot
Match: A0A1C9CX66 ((S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC2 PE=1 SV=1)

HSP 1 Score: 589.3 bits (1518), Expect = 3.1e-167
Identity = 275/394 (69.80%), Postives = 316/394 (80.20%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAA+KKFEED+ PR  +SVAL+IGVTGIVGNSLAEILP+SDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAARKKFEEDDAPRDCQSVALIIGVTGIVGNSLAEILPISDTPGGPWKVYG 60

Query: 61  IARRPRPTWNADHPIEYIQCDISDPEDAEAKLSQLTDVTHLFYVTWTNRPTEIENCEANV 120
           +ARRPRP WNADHP+EYIQCDISD  D   KLS LTDVTH+F+VTW NRPTE E CE N 
Sbjct: 61  VARRPRPAWNADHPVEYIQCDISDASDTHTKLSPLTDVTHIFWVTWANRPTESECCELNG 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYLGSLQSIVDKSVQPHDPPFTEDLPRLEGPNF 180
            MLRNVL ++IP A NL HICLQTG KHY+G  ++     ++PH+PPFTED+PRL  PNF
Sbjct: 121 TMLRNVLNALIPKAANLHHICLQTGHKHYIGPFEAF--GKIKPHEPPFTEDMPRLNAPNF 180

Query: 181 YYSQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLINIVGTLCVYAAICKHEGRTLK 240
           YY+ ED+L E  E  +K  L W+V RP VIFGFSPFS++NI+GTLCVYAAICKHE   LK
Sbjct: 181 YYTLEDMLVEASE--KKAGLNWSVHRPAVIFGFSPFSMMNIIGTLCVYAAICKHENTPLK 240

Query: 241 FPGSKSAWEDFQVGSDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPG+K+AW  + V SDADLIAE  IW AVDPYAKNEAFNC+NGD+FKWKH WKVLAEQF 
Sbjct: 241 FPGTKAAWNCYSVASDADLIAEHQIWAAVDPYAKNEAFNCSNGDLFKWKHLWKVLAEQFG 300

Query: 301 IEDYGFEDGERLT-LVELMKDKSEVWDEIVKENQLQPTKLEEITNWWFTDVIFSLSGNID 360
           +E   F++ E+ T LVE MKDK  VW+EIV+EN L  TKLE +  WWF DVI      +D
Sbjct: 301 VEYAEFDESEKPTSLVERMKDKGPVWEEIVRENGLHTTKLEGVATWWFADVILGGECLLD 360

Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 394
           SMNKSKEHG+LGFRN+KNS IS IDK+KA KIVP
Sbjct: 361 SMNKSKEHGYLGFRNTKNSLISVIDKMKAHKIVP 390

[Close]
BLAST of Bhi09G002679 vs. ExPASy Swiss-Prot
Match: Q6PQJ9 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata OX=49450 PE=1 SV=1)

HSP 1 Score: 583.9 bits (1504), Expect = 1.3e-165
Identity = 269/393 (68.45%), Postives = 319/393 (81.17%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKK+ EED+      SVAL++GVTGI+GNSLAEILPL+DTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKRLEEDDAQPKHSSVALIVGVTGIIGNSLAEILPLADTPGGPWKVYG 60

Query: 61  IARRPRPTWNADHPIEYIQCDISDPEDAEAKLSQLTDVTHLFYVTWTNRPTEIENCEANV 120
           +ARR RP W+ D+PI Y+QCDISDP+D++AKLS LTDVTH+FYVTW NR TE ENCEAN 
Sbjct: 61  VARRTRPAWHEDNPINYVQCDISDPDDSQAKLSPLTDVTHVFYVTWANRSTEQENCEANS 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYLGSLQSIVDKSVQPHDPPFTEDLPRLEGPNF 180
           KM RNVL +VIPN PNL+HI LQTG KHY+G  +S     ++ HDPP+TEDLPRL+  NF
Sbjct: 121 KMFRNVLDAVIPNCPNLKHISLQTGRKHYMGPFESY--GKIESHDPPYTEDLPRLKYMNF 180

Query: 181 YYSQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLINIVGTLCVYAAICKHEGRTLK 240
           YY  ED++ EE+E  +K+ LTW+V RP  IFGFSP+S++N+VGTLCVYAAICKHEG+ L+
Sbjct: 181 YYDLEDIMLEEVE--KKEGLTWSVHRPGNIFGFSPYSMMNLVGTLCVYAAICKHEGKVLR 240

Query: 241 FPGSKSAWEDFQVGSDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           F G K+AW+ +   SDADLIAE HIW AVDPYAKNEAFN +NGDVFKWKHFWKVLAEQF 
Sbjct: 241 FTGCKAAWDGYSDCSDADLIAEHHIWAAVDPYAKNEAFNVSNGDVFKWKHFWKVLAEQFG 300

Query: 301 IEDYGFEDGERLTLVELMKDKSEVWDEIVKENQLQPTKLEEITNWWFTDVIFSLSGNIDS 360
           +    +E+G  L L +LMK K  VW+EIV+EN L PTKL+++  WWF DVI      +DS
Sbjct: 301 VGCGEYEEGVDLKLQDLMKGKEPVWEEIVRENGLTPTKLKDVGIWWFGDVILGNECFLDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 394
           MNKSKEHGFLGFRNSKN+FISWIDK KA+KIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNAFISWIDKAKAYKIVP 389

[Close]
BLAST of Bhi09G002679 vs. ExPASy TrEMBL
Match: A0A5A7T3B3 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold36G001350 PE=3 SV=1)

HSP 1 Score: 771.9 bits (1992), Expect = 1.3e-219
Identity = 356/393 (90.59%), Postives = 377/393 (95.93%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  IARRPRPTWNADHPIEYIQCDISDPEDAEAKLSQLTDVTHLFYVTWTNRPTEIENCEANV 120
           +ARRPRP WNADHPIEYIQCD+SDP+DAE KLSQL DVTHLFYVTWTNR  EI+NCEANV
Sbjct: 61  VARRPRPVWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTIEIDNCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYLGSLQSIVDKSVQPHDPPFTEDLPRLEGPNF 180
           +MLRNVLRSVIPNAPNLRHICLQTGTKHY+GS +S+++KS Q H+PPFTEDLPRLE PNF
Sbjct: 121 EMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESVINKSSQRHEPPFTEDLPRLECPNF 180

Query: 181 YYSQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLINIVGTLCVYAAICKHEGRTLK 240
           YY QEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSL+NIVGTLCVYAAICKHEGR LK
Sbjct: 181 YYKQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240

Query: 241 FPGSKSAWEDFQVGSDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPG+K AWEDFQ  SDADL+AEQHIWTAVDPY+KNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKLAWEDFQAASDADLVAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEDGERLTLVELMKDKSEVWDEIVKENQLQPTKLEEITNWWFTDVIFSLSGNIDS 360
           IE+YGFE+GERLTLV+LMKDKS+VWDEIVKENQLQPTKLEEI NWW+ DVIFS+SGNIDS
Sbjct: 301 IEEYGFEEGERLTLVDLMKDKSDVWDEIVKENQLQPTKLEEIGNWWYVDVIFSVSGNIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 394
           MNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393

[Close]
BLAST of Bhi09G002679 vs. ExPASy TrEMBL
Match: A0A1S3BHA7 (3-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucumis melo OX=3656 GN=LOC103489839 PE=3 SV=1)

HSP 1 Score: 771.9 bits (1992), Expect = 1.3e-219
Identity = 356/393 (90.59%), Postives = 377/393 (95.93%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  IARRPRPTWNADHPIEYIQCDISDPEDAEAKLSQLTDVTHLFYVTWTNRPTEIENCEANV 120
           +ARRPRP WNADHPIEYIQCD+SDP+DAE KLSQL DVTHLFYVTWTNR  EI+NCEANV
Sbjct: 61  VARRPRPVWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTIEIDNCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYLGSLQSIVDKSVQPHDPPFTEDLPRLEGPNF 180
           +MLRNVLRSVIPNAPNLRHICLQTGTKHY+GS +S+++KS Q H+PPFTEDLPRLE PNF
Sbjct: 121 EMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESVINKSSQRHEPPFTEDLPRLECPNF 180

Query: 181 YYSQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLINIVGTLCVYAAICKHEGRTLK 240
           YY QEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSL+NIVGTLCVYAAICKHEGR LK
Sbjct: 181 YYKQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLMNIVGTLCVYAAICKHEGRPLK 240

Query: 241 FPGSKSAWEDFQVGSDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPG+K AWEDFQ  SDADL+AEQHIWTAVDPY+KNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKLAWEDFQAASDADLVAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEDGERLTLVELMKDKSEVWDEIVKENQLQPTKLEEITNWWFTDVIFSLSGNIDS 360
           IE+YGFE+GERLTLV+LMKDKS+VWDEIVKENQLQPTKLEEI NWW+ DVIFS+SGNIDS
Sbjct: 301 IEEYGFEEGERLTLVDLMKDKSDVWDEIVKENQLQPTKLEEIGNWWYVDVIFSVSGNIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 394
           MNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393

[Close]
BLAST of Bhi09G002679 vs. ExPASy TrEMBL
Match: A0A0A0L8Y0 (Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G610790 PE=3 SV=1)

HSP 1 Score: 764.2 bits (1972), Expect = 2.6e-217
Identity = 357/394 (90.61%), Postives = 375/394 (95.18%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  IARRPRPTWNADHPIEYIQCDISDPEDAEAKLSQLTDVTHLFYVTWTNRPTEIENCEANV 120
           +ARRPRP WNADHPIEYIQCD+SDP+DAE KLSQL DVTHLFYVTWTNR TEIENCEANV
Sbjct: 61  VARRPRPAWNADHPIEYIQCDVSDPQDAETKLSQLADVTHLFYVTWTNRTTEIENCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYLGSLQSIVDKSVQPHDPPFTEDLPRLEGPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHY+GS +SI++KS Q HDPPFTEDLPRLE PNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIINKSSQRHDPPFTEDLPRLECPNF 180

Query: 181 YYSQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLINIVGTLCVYAAICKHEGRTLK 240
           YY QEDLL+EEIEQSQKKDLTWAVIRPN+IFGFSPFSL+N+VGTLCVYAAICKHEGR LK
Sbjct: 181 YYKQEDLLWEEIEQSQKKDLTWAVIRPNLIFGFSPFSLMNVVGTLCVYAAICKHEGRPLK 240

Query: 241 FPGSKSAWEDFQVGSDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPG+K AWEDFQV SDADLIAEQHIWTAVDPY+KNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGNKLAWEDFQVASDADLIAEQHIWTAVDPYSKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGF-EDGERLTLVELMKDKSEVWDEIVKENQLQPTKLEEITNWWFTDVIFSLSGNID 360
           IE+YGF E+GE LTLV+LMKDKS+VWDEIVKENQLQ TKLEEI  WWF D IFS+SGNID
Sbjct: 301 IEEYGFDEEGESLTLVDLMKDKSDVWDEIVKENQLQQTKLEEIGTWWFVDSIFSMSGNID 360

Query: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 394
           SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP
Sbjct: 361 SMNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 394

[Close]
BLAST of Bhi09G002679 vs. ExPASy TrEMBL
Match: A0A6J1F7T0 (uncharacterized protein LOC111442928 OS=Cucurbita moschata OX=3662 GN=LOC111442928 PE=3 SV=1)

HSP 1 Score: 762.3 bits (1967), Expect = 9.9e-217
Identity = 352/393 (89.57%), Postives = 375/393 (95.42%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKKKFEE EPPRSFE VALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKKFEEGEPPRSFEGVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  IARRPRPTWNADHPIEYIQCDISDPEDAEAKLSQLTDVTHLFYVTWTNRPTEIENCEANV 120
           +ARR RP+WNADHPIEYIQCDISDPEDAEAKLSQL DVTHLFYVTWT R TE+ENCEANV
Sbjct: 61  VARRSRPSWNADHPIEYIQCDISDPEDAEAKLSQLVDVTHLFYVTWTKRSTELENCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYLGSLQSIVDKSVQPHDPPFTEDLPRLEGPNF 180
           KMLRNVLRSVIPNAPNLRHICLQTGTKHY+GS +SIV+KSVQPHD PFTEDLPRL+ PNF
Sbjct: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYVGSFESIVNKSVQPHDSPFTEDLPRLKCPNF 180

Query: 181 YYSQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLINIVGTLCVYAAICKHEGRTLK 240
           YY+QEDLLFEEIE++Q+KDLTWAVIRPNVIFGFSPF  +NI+GTLC YAAIC+HEGR LK
Sbjct: 181 YYTQEDLLFEEIEKTQRKDLTWAVIRPNVIFGFSPFGWMNIIGTLCAYAAICRHEGRPLK 240

Query: 241 FPGSKSAWEDFQVGSDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPG+KSAWE+F V SDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEDGERLTLVELMKDKSEVWDEIVKENQLQPTKLEEITNWWFTDVIFSLSGNIDS 360
           IEDYGFE+GE+LTLVELMKDKS+VW+EIVKENQLQPT+LEEI NWWF D+I S+SGNIDS
Sbjct: 301 IEDYGFEEGEKLTLVELMKDKSKVWEEIVKENQLQPTRLEEIGNWWFVDIILSISGNIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 394
           MNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393

[Close]
BLAST of Bhi09G002679 vs. ExPASy TrEMBL
Match: A0A6J1IQC1 (3-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111477228 PE=3 SV=1)

HSP 1 Score: 752.7 bits (1942), Expect = 7.9e-214
Identity = 349/393 (88.80%), Postives = 372/393 (94.66%), Query Frame = 0 

Query: 1   MSWWWAGAIGAAKKKFEEDEPPRSFESVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60
           MSWWWAGAIGAAKK F+EDEPPR FE VALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG
Sbjct: 1   MSWWWAGAIGAAKKTFDEDEPPRRFEGVALVIGVTGIVGNSLAEILPLSDTPGGPWKVYG 60

Query: 61  IARRPRPTWNADHPIEYIQCDISDPEDAEAKLSQLTDVTHLFYVTWTNRPTEIENCEANV 120
           +ARR RP+WNADHPIEYIQCDISDPEDAEAKLSQL DVTHLFYVTWT R TE+ENCEANV
Sbjct: 61  VARRSRPSWNADHPIEYIQCDISDPEDAEAKLSQLVDVTHLFYVTWTKRSTELENCEANV 120

Query: 121 KMLRNVLRSVIPNAPNLRHICLQTGTKHYLGSLQSIVDKSVQPHDPPFTEDLPRLEGPNF 180
           KMLRNVLRSVI NAPNLRHICLQTGTKHY+GS +SIV+KSVQP   PFTEDLPRL+ PNF
Sbjct: 121 KMLRNVLRSVISNAPNLRHICLQTGTKHYVGSFESIVNKSVQPPGSPFTEDLPRLKCPNF 180

Query: 181 YYSQEDLLFEEIEQSQKKDLTWAVIRPNVIFGFSPFSLINIVGTLCVYAAICKHEGRTLK 240
           YY+QEDLLFEEIE++Q+KDLTWAVIRPNVIFGFSPFS +NI+GTLC YAAICKHEGR LK
Sbjct: 181 YYTQEDLLFEEIEKTQRKDLTWAVIRPNVIFGFSPFSWMNIIGTLCAYAAICKHEGRPLK 240

Query: 241 FPGSKSAWEDFQVGSDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300
           FPG+KSAWE+F V SDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN
Sbjct: 241 FPGTKSAWEEFSVASDADLIAEQHIWTAVDPYAKNEAFNCNNGDVFKWKHFWKVLAEQFN 300

Query: 301 IEDYGFEDGERLTLVELMKDKSEVWDEIVKENQLQPTKLEEITNWWFTDVIFSLSGNIDS 360
           IEDYGFE+GE+LTLVELMKDKS+VW+EIVKENQLQPT+LEEI NWWF D+I S+SGNIDS
Sbjct: 301 IEDYGFEEGEKLTLVELMKDKSKVWEEIVKENQLQPTRLEEIGNWWFVDIILSISGNIDS 360

Query: 361 MNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 394
           MNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP
Sbjct: 361 MNKSKEHGFLGFRNSKNSFISWIDKIKAFKIVP 393

The following BLAST results are available for this feature:
BLAST of Bhi09G002679 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G24220.12.0e-18575.57NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G24220.21.9e-18375.32NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G58750.14.8e-6235.77NAD(P)-binding Rossmann-fold superfamily protein [more]
BLAST of Bhi09G002679 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9STX22.8e-18475.573-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Arabidopsis thaliana OX=3702 GN=VEP... [more]
A0A221J5W86.9e-17570.74(S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta cataria OX=39347 GN=ISY1 PE=1 ... [more]
A0A221J5X37.7e-17470.48(S)-8-oxocitronellyl enol synthase ISY1 OS=Nepeta racemosa OX=54731 GN=ISY1 PE=1... [more]
A0A1C9CX663.1e-16769.80(S)-8-oxocitronellyl enol synthase CYC2 OS=Camptotheca acuminata OX=16922 GN=CYC... [more]
Q6PQJ91.3e-16568.453-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Digitalis lanata OX=49450 PE=1 SV=1[more]
BLAST of Bhi09G002679 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A5A7T3B31.3e-21990.593-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucumis melo var. makuwa OX=1194695... [more]
A0A1S3BHA71.3e-21990.593-oxo-Delta(4,5)-steroid 5-beta-reductase OS=Cucumis melo OX=3656 GN=LOC10348983... [more]
A0A0A0L8Y02.6e-21790.61Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G610790 P... [more]
A0A6J1F7T09.9e-21789.57uncharacterized protein LOC111442928 OS=Cucurbita moschata OX=3662 GN=LOC1114429... [more]
A0A6J1IQC17.9e-21488.803-oxo-Delta(4,5)-steroid 5-beta-reductase-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001509NAD-dependent epimerase/dehydratasePFAMPF01370Epimerasecoord: 29..214
e-value: 7.1E-6
score: 25.7
NoneNo IPR availableGENE3D3.40.50.720coord: 27..393
e-value: 1.1E-138
score: 464.2
NoneNo IPR availablePANTHERPTHR32487:SF203-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASE-LIKEcoord: 1..393
NoneNo IPR availablePANTHERPTHR324873-OXO-DELTA(4,5)-STEROID 5-BETA-REDUCTASEcoord: 1..393
NoneNo IPR availableCDDcd089485beta-POR_like_SDR_acoord: 28..349
e-value: 4.173E-135
score: 386.986
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 28..342

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Bhi09M002679Bhi09M002679mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009611 response to wounding
biological_process GO:0008202 steroid metabolic process
biological_process GO:0010051 xylem and phloem pattern formation
molecular_function GO:0047568 3-oxo-5-beta-steroid 4-dehydrogenase activity
molecular_function GO:0035671 enone reductase activity
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0003824 catalytic activity