Bhi09G002385 (gene) Wax gourd (B227) v1

Overview


Name	Bhi09G002385
Type	gene
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	12-oxophytodienoate reductase 1
Location	chr9: 75670892 .. 75671288 (+)
RNA-Seq Expression	Bhi09G002385
Synteny	Bhi09G002385

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGCGATTACTCAAGTGGTTAACACTTACAGAGTGGCGGCTAGAAATGCCATAGAAGCTGGTAACATCGAATGAATTGATCCACTACCTAATTATTAATTATTATAACAAAATGAATTAAGAAAACGCCACATTTGAAATTACAAGTTTAATCCTTGAAGTTTTAGGTTTCAACGGAGTTGAGATTAATGGAGCCAATGGGTACTTAATCGATCAGTTTCTCAAAGACCAAGTCAATAGCAGGAGGGATGTTGAATATGGAGGAAACTTAGAAAAGCACTACAGATTTTGTCTAGAAGTTGTGAAAGCTGTGAGTGAAGAGATTGGTAAGGTATTAGATTGTTGCCATTCGCTGATTACCACGAATGCGGCGACTCTGATCCTAAAGCTCTAG

mRNA sequence

ATGGAGGCGATTACTCAAGTGGTTAACACTTACAGAGTGGCGGCTAGAAATGCCATAGAAGCTGGTTTCAACGGAGTTGAGATTAATGGAGCCAATGGGTACTTAATCGATCAGTTTCTCAAAGACCAAGTCAATAGCAGGAGGGATGTTGAATATGGAGGAAACTTAGAAAAGCACTACAGATTTTGTCTAGAAGTTGTGAAAGCTGTGAGTGAAGAGATTGGTAAGGTATTAGATTGTTGCCATTCGCTGATTACCACGAATGCGGCGACTCTGATCCTAAAGCTCTAG

Coding sequence (CDS)

ATGGAGGCGATTACTCAAGTGGTTAACACTTACAGAGTGGCGGCTAGAAATGCCATAGAAGCTGGTTTCAACGGAGTTGAGATTAATGGAGCCAATGGGTACTTAATCGATCAGTTTCTCAAAGACCAAGTCAATAGCAGGAGGGATGTTGAATATGGAGGAAACTTAGAAAAGCACTACAGATTTTGTCTAGAAGTTGTGAAAGCTGTGAGTGAAGAGATTGGTAAGGTATTAGATTGTTGCCATTCGCTGATTACCACGAATGCGGCGACTCTGATCCTAAAGCTCTAG

Protein sequence

MEAITQVVNTYRVAARNAIEAGFNGVEINGANGYLIDQFLKDQVNSRRDVEYGGNLEKHYRFCLEVVKAVSEEIGKVLDCCHSLITTNAATLILKL

Homology

[Close]

BLAST of Bhi09G002385 vs. TAIR 10
Match: AT1G17990.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 99.0 bits (245), Expect = 2.2e-21
Identity = 47/74 (63.51%), Postives = 61/74 (82.43%), Query Frame = 0


Query: 2   EAITQVVNTYRVAARNAIEAGFNGVEINGANGYLIDQFLKDQVNSRRDVEYGGNLEKHYR 61
           + +  +VN +R+AARNAIEAGF+GVE++GA+GYLIDQFLKD+VN R D +YGG+LE   R
Sbjct: 148 DELPAIVNDFRIAARNAIEAGFDGVEVHGAHGYLIDQFLKDKVNDRSD-QYGGSLENRCR 207

Query: 62  FCLEVVKAVSEEIG 76
           F LEV++AV  EIG
Sbjct: 208 FALEVIEAVVNEIG 220

[Close]

BLAST of Bhi09G002385 vs. TAIR 10
Match: AT1G18020.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 99.0 bits (245), Expect = 2.2e-21
Identity = 47/74 (63.51%), Postives = 61/74 (82.43%), Query Frame = 0


Query: 2   EAITQVVNTYRVAARNAIEAGFNGVEINGANGYLIDQFLKDQVNSRRDVEYGGNLEKHYR 61
           + +  +VN +R+AARNAIEAGF+GVE++GA+GYLIDQFLKD+VN R D +YGG+LE   R
Sbjct: 148 DELPAIVNDFRIAARNAIEAGFDGVEVHGAHGYLIDQFLKDKVNDRSD-QYGGSLENRCR 207

Query: 62  FCLEVVKAVSEEIG 76
           F LEV++AV  EIG
Sbjct: 208 FALEVIEAVVNEIG 220

[Close]

BLAST of Bhi09G002385 vs. TAIR 10
Match: AT1G17990.2 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 99.0 bits (245), Expect = 2.2e-21
Identity = 47/74 (63.51%), Postives = 61/74 (82.43%), Query Frame = 0


Query: 2   EAITQVVNTYRVAARNAIEAGFNGVEINGANGYLIDQFLKDQVNSRRDVEYGGNLEKHYR 61
           + +  +VN +R+AARNAIEAGF+GVE++GA+GYLIDQFLKD+VN R D +YGG+LE   R
Sbjct: 140 DELPAIVNDFRIAARNAIEAGFDGVEVHGAHGYLIDQFLKDKVNDRSD-QYGGSLENRCR 199

Query: 62  FCLEVVKAVSEEIG 76
           F LEV++AV  EIG
Sbjct: 200 FALEVIEAVVNEIG 212

[Close]

BLAST of Bhi09G002385 vs. TAIR 10
Match: AT1G76690.1 (12-oxophytodienoate reductase 2 )

HSP 1 Score: 98.6 bits (244), Expect = 2.9e-21
Identity = 48/75 (64.00%), Postives = 63/75 (84.00%), Query Frame = 0


Query: 1   MEAITQVVNTYRVAARNAIEAGFNGVEINGANGYLIDQFLKDQVNSRRDVEYGGNLEKHY 60
           +E I  +VN +R+AARNA+EAGF+GVEI+GA+GYLIDQF+KD+VN R D EYGG+L+   
Sbjct: 157 IEEIPGIVNDFRLAARNAMEAGFDGVEIHGAHGYLIDQFMKDKVNDRTD-EYGGSLQNRC 216

Query: 61  RFCLEVVKAVSEEIG 76
           +F LEVV AV++EIG
Sbjct: 217 KFALEVVDAVAKEIG 230

[Close]

BLAST of Bhi09G002385 vs. TAIR 10
Match: AT1G76680.1 (12-oxophytodienoate reductase 1 )

HSP 1 Score: 98.2 bits (243), Expect = 3.8e-21
Identity = 48/75 (64.00%), Postives = 62/75 (82.67%), Query Frame = 0


Query: 1   MEAITQVVNTYRVAARNAIEAGFNGVEINGANGYLIDQFLKDQVNSRRDVEYGGNLEKHY 60
           +E I  +VN +R+AARNA+EAGF+GVEI+GANGYLIDQF+KD VN R D EYGG+L+   
Sbjct: 155 IEEIPGIVNDFRLAARNAMEAGFDGVEIHGANGYLIDQFMKDTVNDRTD-EYGGSLQNRC 214

Query: 61  RFCLEVVKAVSEEIG 76
           +F LE+V AV++EIG
Sbjct: 215 KFPLEIVDAVAKEIG 228

[Close]

BLAST of Bhi09G002385 vs. ExPASy Swiss-Prot
Match: B9FSC8 (Putative 12-oxophytodienoate reductase 11 OS=Oryza sativa subsp. japonica OX=39947 GN=OPR11 PE=2 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 4.4e-22
Identity = 54/74 (72.97%), Postives = 63/74 (85.14%), Query Frame = 0


Query: 2   EAITQVVNTYRVAARNAIEAGFNGVEINGANGYLIDQFLKDQVNSRRDVEYGGNLEKHYR 61
           + I  VVN YRVAARNAIEAGF+GVEI+GA+GYLIDQFLKDQVN R D +YGG+LE   R
Sbjct: 151 DEIPFVVNDYRVAARNAIEAGFDGVEIHGAHGYLIDQFLKDQVNDRSD-KYGGSLENRCR 210

Query: 62  FCLEVVKAVSEEIG 76
           F LEVV+AV++EIG
Sbjct: 211 FALEVVQAVTDEIG 223

[Close]

BLAST of Bhi09G002385 vs. ExPASy Swiss-Prot
Match: Q9XG54 (12-oxophytodienoate reductase 1 OS=Solanum lycopersicum OX=4081 GN=OPR1 PE=1 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 7.4e-22
Identity = 51/74 (68.92%), Postives = 63/74 (85.14%), Query Frame = 0


Query: 2   EAITQVVNTYRVAARNAIEAGFNGVEINGANGYLIDQFLKDQVNSRRDVEYGGNLEKHYR 61
           + I Q+VN +RVAARNAIEAGF+GVEI+GA+GYLIDQF+KDQVN R D +YGG+LE   R
Sbjct: 160 DEIPQIVNEFRVAARNAIEAGFDGVEIHGAHGYLIDQFMKDQVNDRSD-KYGGSLENRCR 219

Query: 62  FCLEVVKAVSEEIG 76
           F LE+V+AV+ EIG
Sbjct: 220 FALEIVEAVANEIG 232

[Close]

BLAST of Bhi09G002385 vs. ExPASy Swiss-Prot
Match: P0DI08 (Putative 12-oxophytodienoate reductase-like protein 2A OS=Arabidopsis thaliana OX=3702 GN=At1g17990 PE=2 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 3.1e-20
Identity = 47/74 (63.51%), Postives = 61/74 (82.43%), Query Frame = 0


Query: 2   EAITQVVNTYRVAARNAIEAGFNGVEINGANGYLIDQFLKDQVNSRRDVEYGGNLEKHYR 61
           + +  +VN +R+AARNAIEAGF+GVE++GA+GYLIDQFLKD+VN R D +YGG+LE   R
Sbjct: 148 DELPAIVNDFRIAARNAIEAGFDGVEVHGAHGYLIDQFLKDKVNDRSD-QYGGSLENRCR 207

Query: 62  FCLEVVKAVSEEIG 76
           F LEV++AV  EIG
Sbjct: 208 FALEVIEAVVNEIG 220

[Close]

BLAST of Bhi09G002385 vs. ExPASy Swiss-Prot
Match: P0DI09 (Putative 12-oxophytodienoate reductase-like protein 2B OS=Arabidopsis thaliana OX=3702 GN=At1g18020 PE=3 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 3.1e-20
Identity = 47/74 (63.51%), Postives = 61/74 (82.43%), Query Frame = 0


Query: 2   EAITQVVNTYRVAARNAIEAGFNGVEINGANGYLIDQFLKDQVNSRRDVEYGGNLEKHYR 61
           + +  +VN +R+AARNAIEAGF+GVE++GA+GYLIDQFLKD+VN R D +YGG+LE   R
Sbjct: 148 DELPAIVNDFRIAARNAIEAGFDGVEVHGAHGYLIDQFLKDKVNDRSD-QYGGSLENRCR 207

Query: 62  FCLEVVKAVSEEIG 76
           F LEV++AV  EIG
Sbjct: 208 FALEVIEAVVNEIG 220

[Close]

BLAST of Bhi09G002385 vs. ExPASy Swiss-Prot
Match: Q8GYB8 (12-oxophytodienoate reductase 2 OS=Arabidopsis thaliana OX=3702 GN=OPR2 PE=1 SV=2)

HSP 1 Score: 98.6 bits (244), Expect = 4.1e-20
Identity = 48/75 (64.00%), Postives = 63/75 (84.00%), Query Frame = 0


Query: 1   MEAITQVVNTYRVAARNAIEAGFNGVEINGANGYLIDQFLKDQVNSRRDVEYGGNLEKHY 60
           +E I  +VN +R+AARNA+EAGF+GVEI+GA+GYLIDQF+KD+VN R D EYGG+L+   
Sbjct: 157 IEEIPGIVNDFRLAARNAMEAGFDGVEIHGAHGYLIDQFMKDKVNDRTD-EYGGSLQNRC 216

Query: 61  RFCLEVVKAVSEEIG 76
           +F LEVV AV++EIG
Sbjct: 217 KFALEVVDAVAKEIG 230

[Close]

BLAST of Bhi09G002385 vs. ExPASy TrEMBL
Match: A0A6J1IE02 (putative 12-oxophytodienoate reductase 11 OS=Cucurbita maxima OX=3661 GN=LOC111476377 PE=3 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 9.7e-25
Identity = 61/74 (82.43%), Postives = 65/74 (87.84%), Query Frame = 0


Query: 2   EAITQVVNTYRVAARNAIEAGFNGVEINGANGYLIDQFLKDQVNSRRDVEYGGNLEKHYR 61
           E ITQVVN YRVAARNA+EAGF+GVEI+G NGYLIDQFLKDQVN RRD EYGG+LE   R
Sbjct: 173 EGITQVVNDYRVAARNAMEAGFDGVEIHGGNGYLIDQFLKDQVNDRRDDEYGGSLENRCR 232

Query: 62  FCLEVVKAVSEEIG 76
           F LEVVKAVSEEIG
Sbjct: 233 FALEVVKAVSEEIG 246

[Close]

BLAST of Bhi09G002385 vs. ExPASy TrEMBL
Match: A0A6J1FEK3 (putative 12-oxophytodienoate reductase 11 OS=Cucurbita moschata OX=3662 GN=LOC111443362 PE=3 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 1.1e-23
Identity = 59/74 (79.73%), Postives = 64/74 (86.49%), Query Frame = 0


Query: 2   EAITQVVNTYRVAARNAIEAGFNGVEINGANGYLIDQFLKDQVNSRRDVEYGGNLEKHYR 61
           E ITQVVN YRVAARNA+EAGF+GVEI+G NGYLIDQFLKDQVN RRD +YGG+LE   R
Sbjct: 168 EGITQVVNDYRVAARNAMEAGFDGVEIHGGNGYLIDQFLKDQVNDRRDDKYGGSLENRCR 227

Query: 62  FCLEVVKAVSEEIG 76
           F LEVV AVSEEIG
Sbjct: 228 FALEVVNAVSEEIG 241

[Close]

BLAST of Bhi09G002385 vs. ExPASy TrEMBL
Match: A0A6J1D2T5 (putative 12-oxophytodienoate reductase 11 OS=Momordica charantia OX=3673 GN=LOC111016681 PE=3 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 5.3e-23
Identity = 60/74 (81.08%), Postives = 65/74 (87.84%), Query Frame = 0


Query: 2   EAITQVVNTYRVAARNAIEAGFNGVEINGANGYLIDQFLKDQVNSRRDVEYGGNLEKHYR 61
           E I+QVVN +RVAARNAIEAGF+GVEI+G NGYLIDQFL DQVN RRD EYGG+LEK  R
Sbjct: 157 EEISQVVNDFRVAARNAIEAGFDGVEIHGGNGYLIDQFLNDQVNDRRD-EYGGSLEKRCR 216

Query: 62  FCLEVVKAVSEEIG 76
           F LEVVKAVSEEIG
Sbjct: 217 FALEVVKAVSEEIG 229

[Close]

BLAST of Bhi09G002385 vs. ExPASy TrEMBL
Match: A0A6J1AD11 (putative 12-oxophytodienoate reductase 11 OS=Herrania umbratica OX=108875 GN=LOC110416883 PE=3 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 2.9e-21
Identity = 57/74 (77.03%), Postives = 65/74 (87.84%), Query Frame = 0


Query: 2   EAITQVVNTYRVAARNAIEAGFNGVEINGANGYLIDQFLKDQVNSRRDVEYGGNLEKHYR 61
           +AITQVV+ YR AARNA+EAGF+GVEI+GANGYLIDQFLKDQVN R D EYGG+LE   R
Sbjct: 156 DAITQVVDDYRKAARNAMEAGFDGVEIHGANGYLIDQFLKDQVNDRTD-EYGGSLENRCR 215

Query: 62  FCLEVVKAVSEEIG 76
           F LEVVKAV++EIG
Sbjct: 216 FPLEVVKAVADEIG 228

[Close]

BLAST of Bhi09G002385 vs. ExPASy TrEMBL
Match: A0A2R6RME5 (12-oxophytodienoate reductase 2, N-terminally processed like OS=Actinidia chinensis var. chinensis OX=1590841 GN=CEY00_Acc04478 PE=3 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 2.9e-21
Identity = 55/75 (73.33%), Postives = 65/75 (86.67%), Query Frame = 0


Query: 1   MEAITQVVNTYRVAARNAIEAGFNGVEINGANGYLIDQFLKDQVNSRRDVEYGGNLEKHY 60
           +E I +V+N YR+AA+NAIEAGF+GVEI+GANGYLIDQF+KDQVN R D EYGG+LE   
Sbjct: 147 IEEIPKVINDYRIAAKNAIEAGFDGVEIHGANGYLIDQFMKDQVNDRID-EYGGSLENRC 206

Query: 61  RFCLEVVKAVSEEIG 76
           RF LEVVKAV+EEIG
Sbjct: 207 RFALEVVKAVAEEIG 220

The following BLAST results are available for this feature:

BLAST of Bhi09G002385 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G17990.1	2.2e-21	63.51	FMN-linked oxidoreductases superfamily protein	[more]
AT1G18020.1	2.2e-21	63.51	FMN-linked oxidoreductases superfamily protein	[more]
AT1G17990.2	2.2e-21	63.51	FMN-linked oxidoreductases superfamily protein	[more]
AT1G76690.1	2.9e-21	64.00	12-oxophytodienoate reductase 2	[more]
AT1G76680.1	3.8e-21	64.00	12-oxophytodienoate reductase 1	[more]

BLAST of Bhi09G002385 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
B9FSC8	4.4e-22	72.97	Putative 12-oxophytodienoate reductase 11 OS=Oryza sativa subsp. japonica OX=399...	[more]
Q9XG54	7.4e-22	68.92	12-oxophytodienoate reductase 1 OS=Solanum lycopersicum OX=4081 GN=OPR1 PE=1 SV=...	[more]
P0DI08	3.1e-20	63.51	Putative 12-oxophytodienoate reductase-like protein 2A OS=Arabidopsis thaliana O...	[more]
P0DI09	3.1e-20	63.51	Putative 12-oxophytodienoate reductase-like protein 2B OS=Arabidopsis thaliana O...	[more]
Q8GYB8	4.1e-20	64.00	12-oxophytodienoate reductase 2 OS=Arabidopsis thaliana OX=3702 GN=OPR2 PE=1 SV=...	[more]

BLAST of Bhi09G002385 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IE02	9.7e-25	82.43	putative 12-oxophytodienoate reductase 11 OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1FEK3	1.1e-23	79.73	putative 12-oxophytodienoate reductase 11 OS=Cucurbita moschata OX=3662 GN=LOC11...	[more]
A0A6J1D2T5	5.3e-23	81.08	putative 12-oxophytodienoate reductase 11 OS=Momordica charantia OX=3673 GN=LOC1...	[more]
A0A6J1AD11	2.9e-21	77.03	putative 12-oxophytodienoate reductase 11 OS=Herrania umbratica OX=108875 GN=LOC...	[more]
A0A2R6RME5	2.9e-21	73.33	12-oxophytodienoate reductase 2, N-terminally processed like OS=Actinidia chinen...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 1..90 e-value: 9.5E-30 score: 106.0
IPR001155	NADH:flavin oxidoreductase/NADH oxidase, N-terminal	PFAM	PF00724	Oxidored_FMN	coord: 2..76 e-value: 5.8E-26 score: 91.6
None	No IPR available	PANTHER	PTHR22893	NADH OXIDOREDUCTASE-RELATED	coord: 2..76
None	No IPR available	SUPERFAMILY	51395	FMN-linked oxidoreductases	coord: 2..76

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Bhi09M002385	Bhi09M002385	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0010181	FMN binding
molecular_function	GO:0016491	oxidoreductase activity
molecular_function	GO:0003824	catalytic activity