Bhi09G002289 (gene) Wax gourd (B227) v1

BLAST of Bhi09G002289 vs. TAIR 10
Match: AT4G19380.1 (Long-chain fatty alcohol dehydrogenase family protein )

HSP 1 Score: 850.9 bits (2197), Expect = 8.4e-247
Identity = 437/749 (58.34%), Postives = 554/749 (73.97%), Query Frame = 0

Query: 64  MESLAALCDTFLPSV--AATAAADRSVTLFFETSASAAGTPERVGEMIKERVRHPKKWLV 123
           MESL A+CDTF+ S+  +     D  V  +F  SAS  GTP+RV  ++ ER+ HPKKW++
Sbjct: 1   MESLVAICDTFISSIDDSGVGHVDDCVAGYFSASASQTGTPDRVARLMSERLHHPKKWIL 60

Query: 124 RMTLWMLSTWYGTYILCGNESLSNHFPYFERFSKVSQKKREQIAISWSLSYFFLLRMFFR 183
           R  LW+LSTW G+ +LCG  S +  FPYF RF ++ +K+RE+I ++WS SYF LLRM FR
Sbjct: 61  RAGLWLLSTWIGSLVLCGWRSFTGEFPYFRRFCRLPEKRREEILLNWSSSYFSLLRMLFR 120

Query: 184 TIKILCLLAFFTQVDEERNNISWKAIDYPGSDPQFKAKTKHSEASNLKEKGSKSREIDQE 243
           TIK++  L FFTQVDE+  N++WKAI Y G  P     + H    N ++K  K       
Sbjct: 121 TIKLISALVFFTQVDEKGRNLAWKAIGYNGPSPD---HSDHEVELNEEKKKKK------- 180

Query: 244 DDEEELYGPLFKGIVNLQHQQQVQVAVGALRKFGFPVTLLSLSTKP---PNPSMIIKCDA 303
              EE++GPL+ GIV+L+  ++      A R  GF V+    +T      +P M I+CDA
Sbjct: 181 --PEEIFGPLYNGIVDLKSPREAVEKKLAGR--GFAVSNQKRNTNGSSISDPVMKIQCDA 240

Query: 304 VVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGPAMEEMYQASGLVATDNM 363
           VVVGSGSGGGV AGVLA AGYKVLV+E G+Y AR+ LSLLEG AM++MY + GL+AT + 
Sbjct: 241 VVVGSGSGGGVAAGVLAKAGYKVLVIESGNYYARSKLSLLEGQAMDDMYLSGGLLATSDT 300

Query: 364 NVLILAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFRSNLYKEAMEVVCKKMGVQ 423
           NV+ILAGSTVGGGST+NWSASI+TP HV +EW+++ +LE+F S+LY+EAM+VVCK+MGVQ
Sbjct: 301 NVVILAGSTVGGGSTINWSASIKTPEHVMKEWAEKSKLEMFGSDLYREAMDVVCKRMGVQ 360

Query: 424 SEIDNEGFNNAVLRKGCEELGCHAMNIPRNSTSDHYCGWCCLGCKNGRKKGTAETWLVDL 483
                EGFNN VLRKGCE+LG    NIPRN+ SDHYCG+CCLGCK G+K+GT+ETWLVDL
Sbjct: 361 CGFVEEGFNNEVLRKGCEKLGLPVKNIPRNAPSDHYCGFCCLGCKKGQKQGTSETWLVDL 420

Query: 484 VRSGNGAILPGCEAIKIRQEKRDGSKCNRAKGIVFEFGCGSAAKQMCLVESKVTIVACGA 543
           V S NG ILPGC+A ++  +   G K  +A G+ F FG     +++ +VES+VTIVACGA
Sbjct: 421 VESDNGLILPGCQATEVMYDCEQGKK-KKATGVAFAFG-----EEIYVVESRVTIVACGA 480

Query: 544 LNTPPLLKRSGLRNPNIGKNLHLHPVTMAWGYFPAETDKSWPEKDKKSYEGGIMTAMCNI 603
           L TP LLKRSGL+N NIG+NL LHPV MAWG+FP E DK WPEK KKSYEGGIMTAM ++
Sbjct: 481 LRTPHLLKRSGLKNSNIGRNLCLHPVVMAWGWFP-EEDK-WPEKKKKSYEGGIMTAMSSV 540

Query: 604 GSEPDQEPGYGGAVIQTPALHPGLFSILMPWVSGTNIKHRMCKFSRTAHVFALARDKGSG 663
             E +    YG  VIQTPALHPG+FS ++PW S  + K RM KFSRTAH+FAL RDKG+G
Sbjct: 541 VIE-ETHSSYGEMVIQTPALHPGMFSGIIPWTSSKDFKTRMLKFSRTAHIFALLRDKGTG 600

Query: 664 TVKSSSSLSYKMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYREYE 723
           T+ S + + Y ++D DEE+L+ GLE+ LKILAAAGAEEIGTHH++GRS+NV+  S  E E
Sbjct: 601 TIDSKTYIDYNLNDEDEESLKNGLERVLKILAAAGAEEIGTHHSEGRSLNVRTASSLEIE 660

Query: 724 KFVKEESSRPLKDLLTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLFIADSSVFPT 783
           +FV+EESS+PLKDL   ICSAHQMGSCRMGI  ++S V   GETWEVE LF+AD+SVFPT
Sbjct: 661 RFVREESSKPLKDLSGQICSAHQMGSCRMGIRPEESAVRPTGETWEVERLFVADTSVFPT 720

Query: 784 ALGVNPMVTVQAIAYCTAQSILETLKRKR 808
           ALGVNPMVTVQ+IAYC   ++++ LK+K+
Sbjct: 721 ALGVNPMVTVQSIAYCIGLNVVDVLKKKK 726

BLAST of Bhi09G002289 vs. TAIR 10
Match: AT3G23410.1 (fatty alcohol oxidase 3 )

HSP 1 Score: 534.3 bits (1375), Expect = 1.7e-151
Identity = 310/764 (40.58%), Postives = 451/764 (59.03%), Query Frame = 0

Query: 59  LSAAEMESLAALCDTFLPSVAATAAA----------DRSVTLFFETSASAAGTPERVGEM 118
           ++ AEMESLA+ C+  LPSV                  ++  F+ TS S      +  E+
Sbjct: 15  ITVAEMESLASFCEAVLPSVQPPPEELSGEGDNHRNKEALRSFYSTSGSKTPVLRQSIEL 74

Query: 119 IKERVRHPKKWLVRMTLWMLSTWYGTYILCGNESLSNHFPYFERFSKVSQKKREQIAISW 178
           + +R         R+ L++L+T  GT ++CG E L + +P+ E+FS++S +KRE++    
Sbjct: 75  VTKRGTIEAYIATRLILFLLATRLGTLLICGTECLVSRWPFVEKFSELSLEKRERVLQKQ 134

Query: 179 SLSYFFL-LRMFFRTIKILCLLAFFTQVDEERNNISWKAIDYPGSDPQFKAKTKHSEASN 238
             ++    +R  F  IK+  L  FF++V+    N +W+AI Y  +  + K    H+E   
Sbjct: 135 FKNWILTPIRAAFVYIKVAFLFCFFSRVNPNGENPAWEAIGYRVNPDENKPSETHNER-- 194

Query: 239 LKEKGSKSREIDQEDDEEELYGPLFKGIVNLQHQQQVQVAVGALRKFGFPVTLLSLSTKP 298
                                 PL KGIV    + + Q  + +L   G    L       
Sbjct: 195 ----------------------PLEKGIVETMEETE-QTLLESLAHKGLEAVL-----DT 254

Query: 299 PNPSMIIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGPAMEEMY 358
            + ++ IKCD VVVGSGSGGGV A VLA +G KV+VLEKGSY   +     EGP ++++Y
Sbjct: 255 EHDAIRIKCDVVVVGSGSGGGVAASVLAKSGLKVVVLEKGSYFTPSEHRPFEGPGLDKLY 314

Query: 359 QASGLVATDNMNVLILAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFRSNLYKEA 418
           +  G++ + + + ++LAG+TVGGGS VNWSA I+TP  V +EWS++  + LF +  Y  A
Sbjct: 315 ENGGILPSVDGSFMVLAGATVGGGSAVNWSACIKTPKSVLQEWSEDQNIPLFGTKEYLTA 374

Query: 419 MEVVCKKMGVQSEIDNEGFNNAVLRKGCEELGCHAMNIPRNSTSDHYCGWCCLGCKNGRK 478
           MEVV K+MGV  + + E F N +LRKGCE LG +  N+PRNS+  HYCG C  GC+ G K
Sbjct: 375 MEVVWKRMGVTEKCELESFQNQILRKGCENLGFNVENVPRNSSESHYCGSCGYGCRQGDK 434

Query: 479 KGTAETWLVDLVRSGNGA-ILPGCEAIKIRQEK---RDGSKCNRAKGIVFEFGCGSAAKQ 538
           KG+  TWLVD V  G+GA IL GC+A +   EK     G K  +  G++ +   G+ AK 
Sbjct: 435 KGSDRTWLVDAV--GHGAVILTGCKAERFILEKNGSNKGGKQMKCLGVMAKSLNGNIAK- 494

Query: 539 MCLVESKVTIVACGALNTPPLLKRSGLRNPNIGKNLHLHPVTMAWGYFPAETDKSWPEKD 598
           M  +E+KVT+ A GAL TPPL+  SGLRN NIGKNLHLHPV MAWGYFP +   +   K 
Sbjct: 495 MLKIEAKVTVSAGGALLTPPLMISSGLRNRNIGKNLHLHPVLMAWGYFPDKESSNISFKG 554

Query: 599 KKSYEGGIMTAMCNIGSEPDQEPGYGGAVIQTPALHPGLFSILMPWVSGTNIKHRMCKFS 658
             SYEGGI+T++  + SE  +      A+I+TP L PG FS+L PW SG ++K RM ++S
Sbjct: 555 -NSYEGGIITSVSKVLSEDSEV----RAIIETPQLGPGSFSVLTPWTSGLDMKKRMARYS 614

Query: 659 RTAHVFALARDKGSGTVKSSSSLSYKMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNK 718
           RTA +  + RD+GSG VK+   ++Y +D  D +NL+ GL ++L+IL AAGAEE+GTH + 
Sbjct: 615 RTASLITIVRDRGSGEVKTEGRINYTVDKTDRDNLKAGLRESLRILIAAGAEEVGTHRSD 674

Query: 719 GRSINVKKVSYREYEKFV----KEESSRPLKDLLTPICSAHQMGSCRMGIGAKDSVVNQR 778
           G+ +  K V+    ++F+     EE ++ + +      SAHQMGSCR+G   K+  ++  
Sbjct: 675 GQRLICKGVNENSIQEFLDSVSTEEGAKGMTEKWNVYSSAHQMGSCRIGENEKEGAIDLN 734

Query: 779 GETWEVEGLFIADSSVFPTALGVNPMVTVQAIAYCTAQSILETL 804
           GE+WE E LF+ D+S  P+A+GVNPM+TV + AYC +  I +++
Sbjct: 735 GESWEAEKLFVCDASALPSAVGVNPMITVMSTAYCISTRIAKSM 740

BLAST of Bhi09G002289 vs. TAIR 10
Match: AT4G28570.1 (Long-chain fatty alcohol dehydrogenase family protein )

HSP 1 Score: 521.9 bits (1343), Expect = 8.9e-148
Identity = 307/755 (40.66%), Postives = 439/755 (58.15%), Query Frame = 0

Query: 60  SAAEMESLAALCDTFLPSVAATAAADRSVTLFFETSASAAGTPERVGEMIKERVRHPKKW 119
           S ++++SLA +C TFLP    T +  ++V  F   S++     + V EMI +  R     
Sbjct: 28  SPSQIQSLAVICQTFLP--PETTSEQQAVNSFHVASSTQPPFTDEVAEMIVKNGRSEAVK 87

Query: 120 LVRMTLWMLSTWYGTYILCGNESLSNHFPYFERFSKVSQKKREQIAISWSLSYFFLL--R 179
           ++R+ L +LS  +GT +LCG+  L   +P+  +FS++   KRE I  +WS    FLL  R
Sbjct: 88  VLRIILMILSFRFGTLLLCGSLCLDKSWPFVLKFSQLPLDKREAILRNWSRQSGFLLPFR 147

Query: 180 MFFRTIKILCLLAFFTQVDEERNNISWKAIDYPGSDPQFKAKTKHSEASNLKEKGSKSRE 239
           + F   K   L  FF+Q DE   N + +AI Y   D   ++  K SEA            
Sbjct: 148 ITFFLAKFYTLFYFFSQTDENLKNPALEAIGY-CIDGTERSSNKKSEA------------ 207

Query: 240 IDQEDDEEELYGPLFKGIVNLQHQQQVQVAVGALRKFGFPVTLLSLSTKPPNPSMIIKCD 299
                  +E   PL KGI+   H+  V +      K       + ++    +    I+CD
Sbjct: 208 -------DEKRRPLEKGIIETMHESDVTITQSLTEKG------VHVARDDGDNVYRIRCD 267

Query: 300 AVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGPAMEEMYQASGLVATDN 359
           AVVVGSGSGGGV A  LA AG KVLVLEKG+Y    + S LE P+M E+Y+  GL+ T +
Sbjct: 268 AVVVGSGSGGGVAAANLAKAGLKVLVLEKGNYFTAHDYSGLEVPSMLELYEKGGLLTTVD 327

Query: 360 MNVLILAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFRSNLYKEAMEVVCKKMGV 419
              ++LAGS VGGG+ VNWSASI+TP HV +EWS+  +++ F S  Y+ AM+ V  ++GV
Sbjct: 328 GKFMLLAGSAVGGGTAVNWSASIRTPDHVLQEWSEGSKIKFFGSQEYQSAMDEVTIRIGV 387

Query: 420 QSEIDNEGFNNAVLRKGCEELGCHAMNIPRNSTSDHYCGWCCLGCKNGRKKGTAETWLVD 479
                  GF N VLRKGCE LG    ++PRNS  DHYCG C  GC+ G K GT +TWLVD
Sbjct: 388 TERCVKHGFQNQVLRKGCERLGLQVESVPRNSPEDHYCGLCGYGCRAGAKNGTDQTWLVD 447

Query: 480 LVRSGNGAILPGCEAIKIRQEKRDGSKCNRAKGI--VFEFGCGSAAKQMCLVESKVTIVA 539
            V +G   IL G +A +        S   R K    VF    G    +  ++E++VT+ +
Sbjct: 448 AVENG-AVILTGIKAERFVLVDNTSSSNERKKRCVGVFASSVGGKIGKKFIIEARVTVSS 507

Query: 540 CGALNTPPLLKRSGLRNPNIGKNLHLHPVTMAWGYFPAETDKSWPEKDKKSYEGGIMTAM 599
            G+L TPPL+  SGL+NPNIG+NL LHPV M WGYFP E D    E   K YEGGI+T++
Sbjct: 508 AGSLLTPPLMLSSGLKNPNIGRNLKLHPVLMTWGYFP-EKDS---EFSGKMYEGGIITSV 567

Query: 600 CNIGSEPDQEPGYGGAVIQTPALHPGLFSILMPWVSGTNIKHRMCKFSRTAHVFALARDK 659
            ++    D E G   A+++ P + P  ++ L PWVSG ++K RM K+ RTAH+FAL RD 
Sbjct: 568 HHMN---DTESGC-KAILENPLIGPASYAGLSPWVSGPDLKERMIKYGRTAHLFALVRDL 627

Query: 660 GSGTVKSSSSLSYKMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYR 719
           GSG V   + ++Y+    D ENL+ GL +AL++  AAGA E+GT+ + G+ +  + ++  
Sbjct: 628 GSGEVMMENEVTYRTTKKDRENLRAGLRQALRVSVAAGAVEVGTYRSDGQKMKCEAITKE 687

Query: 720 EYEKFVKEESS----RPLKDLLTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLFIA 779
             E+F+ E  +        +  T   SAHQMGSCRMG+ A++  +++ GE+WE EGLF+ 
Sbjct: 688 AMEEFLDEVDAVGGVGTKGEYWTTYFSAHQMGSCRMGVTAEEGALDENGESWEAEGLFVC 745

Query: 780 DSSVFPTALGVNPMVTVQAIAYCTAQSILETLKRK 807
           D S+ P+A+GVNPM+T+Q+ AYC +  I+++L+ K
Sbjct: 748 DGSILPSAVGVNPMITIQSTAYCISSKIVDSLQNK 745

BLAST of Bhi09G002289 vs. TAIR 10
Match: AT1G03990.1 (Long-chain fatty alcohol dehydrogenase family protein )

HSP 1 Score: 510.8 bits (1314), Expect = 2.1e-144
Identity = 288/775 (37.16%), Postives = 444/775 (57.29%), Query Frame = 0

Query: 53  DDLSSHLSAAEMESLAALCDTFLPSVAATA----------AADRSVTLFFETSASAAGTP 112
           +  S   S +++++L+++CD  +P V   +            D  ++ F  +S+ +   P
Sbjct: 22  ESFSHGFSKSDLQALSSICDAIMPPVPLESLNLEMKLKVLRNDALLSFFKSSSSESHVRP 81

Query: 113 ERVGEMIKERVRHPKKWLVRMTLWMLSTWYGTYILCGNESL-SNHFPYFERFSKVSQKKR 172
           + V E++  +       +VR+ L +L+   GT +LCG   L   H+P+  +FS++S +KR
Sbjct: 82  DEVAELLATKAIPLTVLVVRIVLRILTFRLGTLLLCGLVCLDKKHWPFLLKFSEMSLEKR 141

Query: 173 EQIAISWSLSYFF-LLRMFFRTIKILCLLAFFTQVDEERNNISWKAIDYPGSDPQFKAKT 232
           E++   W+  ++  L R+ F  IK + L  +FT  +E   N  W AI+Y           
Sbjct: 142 EKVLQRWNTQWYNPLARIGFMMIKAIFLFYYFTWTNENSENPVWDAINY----------- 201

Query: 233 KHSEASNLKEKGSKSREIDQEDDEEELYGPLFKGIVNLQHQQQVQVAVGALRKFGFPVTL 292
                         S EI + +D E+   PL +GI+    + ++ +    + K       
Sbjct: 202 --------------SVEIGENEDMEQKERPLDEGIIETAKEDEMTIKQRMINKG------ 261

Query: 293 LSLSTKPPNPSMIIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEG 352
           L ++      +  I+CDAVVVGSG GGGV A +LA +G +V+V+EKG+Y A  + S LEG
Sbjct: 262 LKVTEDRERDTYKIECDAVVVGSGCGGGVAAAILAKSGLRVVVIEKGNYFAPRDYSALEG 321

Query: 353 PAMEEMYQASGLVATDNMNVLILAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFR 412
           P+M E+++++ L+ T +     +AGSTVGGGS VNW+AS++TP  +  EWS    + ++ 
Sbjct: 322 PSMFELFESNSLMMTHDGRFRFMAGSTVGGGSVVNWAASLKTPDAIIEEWSVHRGISIYS 381

Query: 413 SNLYKEAMEVVCKKMGVQSEIDNEGFNNAVLRKGCEELGCHAMNIPRNSTSDHYCGWCCL 472
           S  YK AM +VCK++GV  +I  EGF N +LRKGCE+LG     +PRNST  HYCG C  
Sbjct: 382 SEKYKAAMGIVCKRLGVTEKIIREGFQNQILRKGCEKLGLDVTIVPRNSTEKHYCGSCSY 441

Query: 473 GCKNGRKKGTAETWLVDLVRSGNGAILPGCEAIKIRQEKRDGSK----CNRAKGIVFEFG 532
           GC  G K+GT  TWLVD V + N  IL  C+A K+     D +K      R + +     
Sbjct: 442 GCPTGEKRGTDSTWLVDAV-NNNAVILTQCKAEKLILADNDANKREESGRRKRCLGVAAS 501

Query: 533 CGSAAKQMCLVESKVTIVACGALNTPPLLKRSGLRNPNIGKNLHLHPVTMAWGYFPAETD 592
                ++   + +KVTIVACG+L TP LL  SGL+N NI + LH+HP+ MAWGYFP +  
Sbjct: 502 LSHQTRKKLQINAKVTIVACGSLKTPGLLASSGLKNSNISRGLHIHPIMMAWGYFPEKNS 561

Query: 593 KSWPEKDKKSYEGGIMTAMCNIGSEPDQEPGYGGAVIQTPALHPGLFSILMPWVSGTNIK 652
               E +  ++EG I+T++  +       P      ++TPA+ PG F+ L PWVSG+++K
Sbjct: 562 ----ELEGAAHEGEIVTSLHYVHPMDSTTP---NITLETPAIGPGTFAALTPWVSGSDMK 621

Query: 653 HRMCKFSRTAHVFALARDKGSGTVKSSSSLSYKMDDVDEENLQKGLEKALKILAAAGAEE 712
            RM K++RTAH+FA+ RD+G G VK    + Y++   DEENL  GL++AL+IL AAGA E
Sbjct: 622 ERMAKYARTAHIFAMVRDEGVGEVK-GDIVKYRLTKADEENLTIGLKQALRILVAAGAAE 681

Query: 713 IGTHHNKGRSINVKKVSYREYEKFVKEESSRP----LKDLLTPICSAHQMGSCRMGIGAK 772
           +GT+ + G+ +    +  ++ E F+   ++ P    +    T   +AHQ+G CRMG   K
Sbjct: 682 VGTYRSDGQRMKCDGIKQKDLEAFLDTVNAPPGVVSMSKHWTQSFTAHQIGCCRMGATEK 741

Query: 773 DSVVNQRGETWEVEGLFIADSSVFPTALGVNPMVTVQAIAYCTAQSILETLKRKR 808
           +  ++ +GE+WE E L++ D+SV PTALGVNPM+TVQ+ AYC +  I E +K+++
Sbjct: 742 EGAIDGKGESWEAEDLYVCDASVLPTALGVNPMITVQSTAYCISNRIAELMKKRK 756

BLAST of Bhi09G002289 vs. ExPASy Swiss-Prot
Match: O65709 (Long-chain-alcohol oxidase FAO4A OS=Arabidopsis thaliana OX=3702 GN=FAO4A PE=3 SV=2)

HSP 1 Score: 850.9 bits (2197), Expect = 1.2e-245
Identity = 437/749 (58.34%), Postives = 554/749 (73.97%), Query Frame = 0

Query: 64  MESLAALCDTFLPSV--AATAAADRSVTLFFETSASAAGTPERVGEMIKERVRHPKKWLV 123
           MESL A+CDTF+ S+  +     D  V  +F  SAS  GTP+RV  ++ ER+ HPKKW++
Sbjct: 1   MESLVAICDTFISSIDDSGVGHVDDCVAGYFSASASQTGTPDRVARLMSERLHHPKKWIL 60

Query: 124 RMTLWMLSTWYGTYILCGNESLSNHFPYFERFSKVSQKKREQIAISWSLSYFFLLRMFFR 183
           R  LW+LSTW G+ +LCG  S +  FPYF RF ++ +K+RE+I ++WS SYF LLRM FR
Sbjct: 61  RAGLWLLSTWIGSLVLCGWRSFTGEFPYFRRFCRLPEKRREEILLNWSSSYFSLLRMLFR 120

Query: 184 TIKILCLLAFFTQVDEERNNISWKAIDYPGSDPQFKAKTKHSEASNLKEKGSKSREIDQE 243
           TIK++  L FFTQVDE+  N++WKAI Y G  P     + H    N ++K  K       
Sbjct: 121 TIKLISALVFFTQVDEKGRNLAWKAIGYNGPSPD---HSDHEVELNEEKKKKK------- 180

Query: 244 DDEEELYGPLFKGIVNLQHQQQVQVAVGALRKFGFPVTLLSLSTKP---PNPSMIIKCDA 303
              EE++GPL+ GIV+L+  ++      A R  GF V+    +T      +P M I+CDA
Sbjct: 181 --PEEIFGPLYNGIVDLKSPREAVEKKLAGR--GFAVSNQKRNTNGSSISDPVMKIQCDA 240

Query: 304 VVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGPAMEEMYQASGLVATDNM 363
           VVVGSGSGGGV AGVLA AGYKVLV+E G+Y AR+ LSLLEG AM++MY + GL+AT + 
Sbjct: 241 VVVGSGSGGGVAAGVLAKAGYKVLVIESGNYYARSKLSLLEGQAMDDMYLSGGLLATSDT 300

Query: 364 NVLILAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFRSNLYKEAMEVVCKKMGVQ 423
           NV+ILAGSTVGGGST+NWSASI+TP HV +EW+++ +LE+F S+LY+EAM+VVCK+MGVQ
Sbjct: 301 NVVILAGSTVGGGSTINWSASIKTPEHVMKEWAEKSKLEMFGSDLYREAMDVVCKRMGVQ 360

Query: 424 SEIDNEGFNNAVLRKGCEELGCHAMNIPRNSTSDHYCGWCCLGCKNGRKKGTAETWLVDL 483
                EGFNN VLRKGCE+LG    NIPRN+ SDHYCG+CCLGCK G+K+GT+ETWLVDL
Sbjct: 361 CGFVEEGFNNEVLRKGCEKLGLPVKNIPRNAPSDHYCGFCCLGCKKGQKQGTSETWLVDL 420

Query: 484 VRSGNGAILPGCEAIKIRQEKRDGSKCNRAKGIVFEFGCGSAAKQMCLVESKVTIVACGA 543
           V S NG ILPGC+A ++  +   G K  +A G+ F FG     +++ +VES+VTIVACGA
Sbjct: 421 VESDNGLILPGCQATEVMYDCEQGKK-KKATGVAFAFG-----EEIYVVESRVTIVACGA 480

Query: 544 LNTPPLLKRSGLRNPNIGKNLHLHPVTMAWGYFPAETDKSWPEKDKKSYEGGIMTAMCNI 603
           L TP LLKRSGL+N NIG+NL LHPV MAWG+FP E DK WPEK KKSYEGGIMTAM ++
Sbjct: 481 LRTPHLLKRSGLKNSNIGRNLCLHPVVMAWGWFP-EEDK-WPEKKKKSYEGGIMTAMSSV 540

Query: 604 GSEPDQEPGYGGAVIQTPALHPGLFSILMPWVSGTNIKHRMCKFSRTAHVFALARDKGSG 663
             E +    YG  VIQTPALHPG+FS ++PW S  + K RM KFSRTAH+FAL RDKG+G
Sbjct: 541 VIE-ETHSSYGEMVIQTPALHPGMFSGIIPWTSSKDFKTRMLKFSRTAHIFALLRDKGTG 600

Query: 664 TVKSSSSLSYKMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYREYE 723
           T+ S + + Y ++D DEE+L+ GLE+ LKILAAAGAEEIGTHH++GRS+NV+  S  E E
Sbjct: 601 TIDSKTYIDYNLNDEDEESLKNGLERVLKILAAAGAEEIGTHHSEGRSLNVRTASSLEIE 660

Query: 724 KFVKEESSRPLKDLLTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLFIADSSVFPT 783
           +FV+EESS+PLKDL   ICSAHQMGSCRMGI  ++S V   GETWEVE LF+AD+SVFPT
Sbjct: 661 RFVREESSKPLKDLSGQICSAHQMGSCRMGIRPEESAVRPTGETWEVERLFVADTSVFPT 720

Query: 784 ALGVNPMVTVQAIAYCTAQSILETLKRKR 808
           ALGVNPMVTVQ+IAYC   ++++ LK+K+
Sbjct: 721 ALGVNPMVTVQSIAYCIGLNVVDVLKKKK 726

BLAST of Bhi09G002289 vs. ExPASy Swiss-Prot
Match: B5WWZ8 (Long-chain-alcohol oxidase FAO1 OS=Lotus japonicus OX=34305 GN=FAO1 PE=1 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 1.5e-163
Identity = 325/762 (42.65%), Postives = 457/762 (59.97%), Query Frame = 0

Query: 60  SAAEMESLAALCDTFLPSVAATA--------AADRSVTLFFETSASAAGTPERVGEMIKE 119
           S++EMESLA++C+  LP +   A         +   V  F+ TSAS    P  V E++ +
Sbjct: 23  SSSEMESLASICEVILPPLPMDALKIKKHDDVSIEDVEFFWNTSASHYPIPHEVAEILSK 82

Query: 120 RVRHPKKWLVRMTLWMLSTWYGTYILCGNESLSNHFPYFERFSKVSQKKREQIAISWSLS 179
           R       L+R+ LW+L+T  GT++LCG    S  +P+   FS +S KKRE++   W  +
Sbjct: 83  RSLIEAVILIRVVLWLLATRLGTFLLCGLLCFSEKWPFINNFSSMSLKKRERVMQWWLKN 142

Query: 180 YFFL-LRMFFRTIKILCLLAFFTQVDEERNNISWKAIDYPGSDPQFKAKTKHSEASNLKE 239
            F   +R+ F  +K+LCL A+FT VDE  +N +WKAI Y  S  +   K  +S    L E
Sbjct: 143 RFITPIRLAFAYLKVLCLFAYFTWVDENGDNPAWKAIGYEVSADE---KLPNSSNGRLLE 202

Query: 240 KGSKSREIDQEDDEEELYGPLFKGIVNLQHQQQVQVAVGALRKFGFPVTLLSLSTKPPNP 299
                                 KGIV   H+    +      K G  VTL S      N 
Sbjct: 203 ----------------------KGIVETMHETNSTLQQSLTEK-GLNVTLDS-----RNN 262

Query: 300 SMIIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGPAMEEMYQAS 359
            + +KCDA+VVGSG GGGV A VL+ AG+KV+VLEKG+Y A  + S LEGP+M+++Y+  
Sbjct: 263 ILKVKCDAIVVGSGCGGGVAASVLSGAGHKVVVLEKGNYFAPRDYSSLEGPSMDQLYETG 322

Query: 360 GLVATDNMNVLILAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFRSNLYKEAMEV 419
           G++A+ +  +L+LAGSTVGGGS VNWSA I+TP  V +EWS+ + L LF ++ Y  AME 
Sbjct: 323 GILASVDSRILLLAGSTVGGGSAVNWSACIKTPHEVLKEWSENHNLSLFSTSEYLSAMET 382

Query: 420 VCKKMGVQSEIDNEGFNNAVLRKGCEELGCHAMNIPRNSTSDHYCGWCCLGCKNGRKKGT 479
           VC+++GV     +EGF N VLRKGC+ LG     +PRNS+ +H+CG C  GC  G K+GT
Sbjct: 383 VCERIGVTESCTHEGFQNQVLRKGCQNLGLKVDYVPRNSSGNHFCGSCGYGCPKGEKQGT 442

Query: 480 AETWLVDLVRSGNGAILPGCEAIK-IRQEKRDGSKCNRAKGIVFEFGCGSAAKQMCLVES 539
            +TWLVD V +G   I+ GC+A + + +  R+GS   +    V      S       +E+
Sbjct: 443 QDTWLVDAVENG-AVIITGCKAERFLLENNRNGSGRKKKCLGVLAKALSSRVTMKLQIEA 502

Query: 540 KVTIVACGALNTPPLLKRSGLRNPNIGKNLHLHPVTMAWGYFPAETDKSWPEKDKKSYEG 599
           KVTI A GAL TPPL+  SGL+N NIGKNLHLHPV M WGYFP  T     E   K +EG
Sbjct: 503 KVTISAGGALLTPPLMISSGLKNKNIGKNLHLHPVLMTWGYFPESTS----ELKGKIFEG 562

Query: 600 GIMTAMCNIGSEPDQEPGYGGAVIQTPALHPGLFSILMPWVSGTNIKHRMCKFSRTAHVF 659
           GI+T++  + S          A+I+TP+L PG F+ L PW SG + K RM  + RTAH+ 
Sbjct: 563 GIITSVHKVPSRDSNSYSDTRAIIETPSLGPGSFASLCPWESGLDFKERMLNYPRTAHLI 622

Query: 660 ALARDKGSGTVKSSSSLSYKMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINV 719
            + RDK  G V +   +SYK+   D+EN++ GL++AL+IL AAGA E+GTH + G+ +  
Sbjct: 623 TIIRDKACGQVTTEGRVSYKLSAFDKENMKCGLQQALRILKAAGAVEVGTHRSDGQRVKC 682

Query: 720 KKVSYREYEKFVKEESSRPLKDLLTP------ICSAHQMGSCRMGIGAKDSVVNQRGETW 779
            +VS  E  +F+  +S  P++  L+P        SAHQMGSCRMG+  K+  V++ GE+W
Sbjct: 683 GEVSENEMAEFI--DSVCPMEGALSPGENWNIYTSAHQMGSCRMGMNEKEGAVDENGESW 742

Query: 780 EVEGLFIADSSVFPTALGVNPMVTVQAIAYCTAQSILETLKR 806
           E +GLF+ D+S+ PTA+GVNPM+T+Q+ AYC +  +++ LKR
Sbjct: 743 EAQGLFVCDASLLPTAVGVNPMITIQSTAYCISNRVVDYLKR 746

BLAST of Bhi09G002289 vs. ExPASy Swiss-Prot
Match: B5WWZ9 (Long-chain-alcohol oxidase FAO2 OS=Lotus japonicus OX=34305 GN=FAO2 PE=2 SV=1)

HSP 1 Score: 548.5 bits (1412), Expect = 1.3e-154
Identity = 312/770 (40.52%), Postives = 458/770 (59.48%), Query Frame = 0

Query: 56  SSHLSAAEMESLAALCDTFLPSVAA-----TAAADRSVTLFFETSASAAGTPERVGEMI- 115
           S  LS+++M  +AA+C+   PS         ++ D++++ F+  S S A  P+   E++ 
Sbjct: 24  SHGLSSSQMHVIAAICEALFPSQPLDSQNNQSSVDKALSAFYTASGSQAPLPDEAAELLF 83

Query: 116 KERVRHPKKW-LVRMTLWMLSTWYGTYILCGNESLSNHFPYFERFSKVSQKKREQIAISW 175
           K     P+   LV   L +LS   GT +LCG   L   +P+  +FS++  +KRE+I   W
Sbjct: 84  KFNRSFPEALSLVSWVLLILSFRLGTLLLCGTLCLDWRWPFIHKFSEIPLEKREEILKRW 143

Query: 176 SLSYFFL-LRMFFRTIKILCLLAFFTQVDEERNNISWKAIDYPGSDPQFKAKTKHSEASN 235
           S    ++ LR+ F   K++C    F++ D   +N  WKAI Y                  
Sbjct: 144 SREKCWIPLRLVFVLTKLVCFYNLFSRADVNGHNPIWKAIGY------------------ 203

Query: 236 LKEKGSKSREIDQEDDEEELYGPLFKGIVNLQHQQQVQVAVGALRKFGFPVTLLSLSTKP 295
                    ++D  +   +   PL +G++   ++      + +L + G  V      T+ 
Sbjct: 204 ---------QVDTREKLTQKKRPLQEGLIETMYETD-STLIQSLTEKGLEV------TED 263

Query: 296 PNPSMI-IKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGPAMEEM 355
              +M  IKCDAV+VGSG GGGV A VLAN+G+KV++LEKG Y    + S LEGP+M E+
Sbjct: 264 LEQNMYKIKCDAVIVGSGCGGGVAAAVLANSGHKVIILEKGEYFVSHDYSSLEGPSMNEL 323

Query: 356 YQASGLVATDNMNVLILAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFRSNLYKE 415
           Y++ G++ + +  ++ILAGST+GG S +NWSA I+TP  V REWS+++++ LF S  Y+ 
Sbjct: 324 YESGGILPSLDGKMMILAGSTLGGXSAINWSACIRTPDSVLREWSEKHKIPLFASPDYQS 383

Query: 416 AMEVVCKKMGVQSEIDNEGFNNAVLRKGCEELGCHAMNIPRNSTSDHYCGWCCLGCKNGR 475
           AM+ VC+++GV    + E F N +LR+GC ++G     +  NS++DHYCG CC GC+ G 
Sbjct: 384 AMDTVCRRIGVTENCNKESFQNQILRQGCAKIGFKVEPVAINSSADHYCGSCCYGCRTGD 443

Query: 476 KKGTAETWLVDLVRSGNGA-ILPGCEAIKIRQEKRDGSKCNRAK---GIVFEFGCGSAAK 535
           KKGT  TWLVD V  GNGA IL GC+A K+    +DG    + K   G++      S   
Sbjct: 444 KKGTESTWLVDAV--GNGAVILTGCKAEKLNFTLKDGDNGTKRKTCSGVIASATWRSKVT 503

Query: 536 QMCLVESKVTIVACGALNTPPLLKRSGLRNPNIGKNLHLHPVTMAWGYFPAETDKSWPEK 595
           +   +ESKVTI ACG+L+TPPL+  SGL+NPNIGKNLHLHP   AWGYFP +        
Sbjct: 504 KKLQIESKVTISACGSLSTPPLMISSGLKNPNIGKNLHLHPCQFAWGYFPEDMTNF---- 563

Query: 596 DKKSYEGGIMTAMCNIGSEPDQEPGYGGAVIQTPALHPGLFSILMPWVSGTNIKHRMCKF 655
              +YEGGI+T++  +  E          +I+ PAL PG FS L+PWVSG ++K RM K+
Sbjct: 564 SGNNYEGGIITSIHKVFEE--DSTSTPRIIIEAPALGPGSFSALVPWVSGLDVKERMVKY 623

Query: 656 SRTAHVFALARDKGSGTVKSSSSLSYKMDDVDEENLQKGLEKALKILAAAGAEEIGTHHN 715
           +RTA++FAL RD GSG VK+   +SYK+D +D E+LQ GL KAL+IL AAGA E+GT+ +
Sbjct: 624 ARTANLFALVRDHGSGEVKAEGRISYKLDKIDRESLQTGLRKALRILVAAGAVEVGTYRS 683

Query: 716 KGRSINVKKVSYREYEKF------VKEESSRPLKDLLTPICSAHQMGSCRMGIGAKDSVV 775
            G+ I  + +   + E+F      V   SSR   ++ T   SAHQM SCRM    ++  V
Sbjct: 684 DGQRIKCRGIKESDLEEFLDSVRVVGGPSSR--NEVWTVFTSAHQMTSCRMSATEEEGAV 743

Query: 776 NQRGETWEVEGLFIADSSVFPTALGVNPMVTVQAIAYCTAQSILETLKRK 807
           ++ GE+WE +GL++ D SV P+A+GVNPM+T+Q+ AYC A +I E+LK++
Sbjct: 744 DENGESWEAKGLYVCDGSVLPSAVGVNPMITIQSTAYCIASNIAESLKKQ 749

BLAST of Bhi09G002289 vs. ExPASy Swiss-Prot
Match: Q9LW56 (Long-chain-alcohol oxidase FAO3 OS=Arabidopsis thaliana OX=3702 GN=FAO3 PE=1 SV=1)

HSP 1 Score: 534.3 bits (1375), Expect = 2.4e-150
Identity = 310/764 (40.58%), Postives = 451/764 (59.03%), Query Frame = 0

Query: 59  LSAAEMESLAALCDTFLPSVAATAAA----------DRSVTLFFETSASAAGTPERVGEM 118
           ++ AEMESLA+ C+  LPSV                  ++  F+ TS S      +  E+
Sbjct: 15  ITVAEMESLASFCEAVLPSVQPPPEELSGEGDNHRNKEALRSFYSTSGSKTPVLRQSIEL 74

Query: 119 IKERVRHPKKWLVRMTLWMLSTWYGTYILCGNESLSNHFPYFERFSKVSQKKREQIAISW 178
           + +R         R+ L++L+T  GT ++CG E L + +P+ E+FS++S +KRE++    
Sbjct: 75  VTKRGTIEAYIATRLILFLLATRLGTLLICGTECLVSRWPFVEKFSELSLEKRERVLQKQ 134

Query: 179 SLSYFFL-LRMFFRTIKILCLLAFFTQVDEERNNISWKAIDYPGSDPQFKAKTKHSEASN 238
             ++    +R  F  IK+  L  FF++V+    N +W+AI Y  +  + K    H+E   
Sbjct: 135 FKNWILTPIRAAFVYIKVAFLFCFFSRVNPNGENPAWEAIGYRVNPDENKPSETHNER-- 194

Query: 239 LKEKGSKSREIDQEDDEEELYGPLFKGIVNLQHQQQVQVAVGALRKFGFPVTLLSLSTKP 298
                                 PL KGIV    + + Q  + +L   G    L       
Sbjct: 195 ----------------------PLEKGIVETMEETE-QTLLESLAHKGLEAVL-----DT 254

Query: 299 PNPSMIIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGPAMEEMY 358
            + ++ IKCD VVVGSGSGGGV A VLA +G KV+VLEKGSY   +     EGP ++++Y
Sbjct: 255 EHDAIRIKCDVVVVGSGSGGGVAASVLAKSGLKVVVLEKGSYFTPSEHRPFEGPGLDKLY 314

Query: 359 QASGLVATDNMNVLILAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFRSNLYKEA 418
           +  G++ + + + ++LAG+TVGGGS VNWSA I+TP  V +EWS++  + LF +  Y  A
Sbjct: 315 ENGGILPSVDGSFMVLAGATVGGGSAVNWSACIKTPKSVLQEWSEDQNIPLFGTKEYLTA 374

Query: 419 MEVVCKKMGVQSEIDNEGFNNAVLRKGCEELGCHAMNIPRNSTSDHYCGWCCLGCKNGRK 478
           MEVV K+MGV  + + E F N +LRKGCE LG +  N+PRNS+  HYCG C  GC+ G K
Sbjct: 375 MEVVWKRMGVTEKCELESFQNQILRKGCENLGFNVENVPRNSSESHYCGSCGYGCRQGDK 434

Query: 479 KGTAETWLVDLVRSGNGA-ILPGCEAIKIRQEK---RDGSKCNRAKGIVFEFGCGSAAKQ 538
           KG+  TWLVD V  G+GA IL GC+A +   EK     G K  +  G++ +   G+ AK 
Sbjct: 435 KGSDRTWLVDAV--GHGAVILTGCKAERFILEKNGSNKGGKQMKCLGVMAKSLNGNIAK- 494

Query: 539 MCLVESKVTIVACGALNTPPLLKRSGLRNPNIGKNLHLHPVTMAWGYFPAETDKSWPEKD 598
           M  +E+KVT+ A GAL TPPL+  SGLRN NIGKNLHLHPV MAWGYFP +   +   K 
Sbjct: 495 MLKIEAKVTVSAGGALLTPPLMISSGLRNRNIGKNLHLHPVLMAWGYFPDKESSNISFKG 554

Query: 599 KKSYEGGIMTAMCNIGSEPDQEPGYGGAVIQTPALHPGLFSILMPWVSGTNIKHRMCKFS 658
             SYEGGI+T++  + SE  +      A+I+TP L PG FS+L PW SG ++K RM ++S
Sbjct: 555 -NSYEGGIITSVSKVLSEDSEV----RAIIETPQLGPGSFSVLTPWTSGLDMKKRMARYS 614

Query: 659 RTAHVFALARDKGSGTVKSSSSLSYKMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNK 718
           RTA +  + RD+GSG VK+   ++Y +D  D +NL+ GL ++L+IL AAGAEE+GTH + 
Sbjct: 615 RTASLITIVRDRGSGEVKTEGRINYTVDKTDRDNLKAGLRESLRILIAAGAEEVGTHRSD 674

Query: 719 GRSINVKKVSYREYEKFV----KEESSRPLKDLLTPICSAHQMGSCRMGIGAKDSVVNQR 778
           G+ +  K V+    ++F+     EE ++ + +      SAHQMGSCR+G   K+  ++  
Sbjct: 675 GQRLICKGVNENSIQEFLDSVSTEEGAKGMTEKWNVYSSAHQMGSCRIGENEKEGAIDLN 734

Query: 779 GETWEVEGLFIADSSVFPTALGVNPMVTVQAIAYCTAQSILETL 804
           GE+WE E LF+ D+S  P+A+GVNPM+TV + AYC +  I +++
Sbjct: 735 GESWEAEKLFVCDASALPSAVGVNPMITVMSTAYCISTRIAKSM 740

BLAST of Bhi09G002289 vs. ExPASy Swiss-Prot
Match: Q94BP3 (Long-chain-alcohol oxidase FAO4B OS=Arabidopsis thaliana OX=3702 GN=FAO4B PE=2 SV=2)

HSP 1 Score: 521.9 bits (1343), Expect = 1.3e-146
Identity = 307/755 (40.66%), Postives = 439/755 (58.15%), Query Frame = 0

Query: 60  SAAEMESLAALCDTFLPSVAATAAADRSVTLFFETSASAAGTPERVGEMIKERVRHPKKW 119
           S ++++SLA +C TFLP    T +  ++V  F   S++     + V EMI +  R     
Sbjct: 28  SPSQIQSLAVICQTFLP--PETTSEQQAVNSFHVASSTQPPFTDEVAEMIVKNGRSEAVK 87

Query: 120 LVRMTLWMLSTWYGTYILCGNESLSNHFPYFERFSKVSQKKREQIAISWSLSYFFLL--R 179
           ++R+ L +LS  +GT +LCG+  L   +P+  +FS++   KRE I  +WS    FLL  R
Sbjct: 88  VLRIILMILSFRFGTLLLCGSLCLDKSWPFVLKFSQLPLDKREAILRNWSRQSGFLLPFR 147

Query: 180 MFFRTIKILCLLAFFTQVDEERNNISWKAIDYPGSDPQFKAKTKHSEASNLKEKGSKSRE 239
           + F   K   L  FF+Q DE   N + +AI Y   D   ++  K SEA            
Sbjct: 148 ITFFLAKFYTLFYFFSQTDENLKNPALEAIGY-CIDGTERSSNKKSEA------------ 207

Query: 240 IDQEDDEEELYGPLFKGIVNLQHQQQVQVAVGALRKFGFPVTLLSLSTKPPNPSMIIKCD 299
                  +E   PL KGI+   H+  V +      K       + ++    +    I+CD
Sbjct: 208 -------DEKRRPLEKGIIETMHESDVTITQSLTEKG------VHVARDDGDNVYRIRCD 267

Query: 300 AVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGPAMEEMYQASGLVATDN 359
           AVVVGSGSGGGV A  LA AG KVLVLEKG+Y    + S LE P+M E+Y+  GL+ T +
Sbjct: 268 AVVVGSGSGGGVAAANLAKAGLKVLVLEKGNYFTAHDYSGLEVPSMLELYEKGGLLTTVD 327

Query: 360 MNVLILAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFRSNLYKEAMEVVCKKMGV 419
              ++LAGS VGGG+ VNWSASI+TP HV +EWS+  +++ F S  Y+ AM+ V  ++GV
Sbjct: 328 GKFMLLAGSAVGGGTAVNWSASIRTPDHVLQEWSEGSKIKFFGSQEYQSAMDEVTIRIGV 387

Query: 420 QSEIDNEGFNNAVLRKGCEELGCHAMNIPRNSTSDHYCGWCCLGCKNGRKKGTAETWLVD 479
                  GF N VLRKGCE LG    ++PRNS  DHYCG C  GC+ G K GT +TWLVD
Sbjct: 388 TERCVKHGFQNQVLRKGCERLGLQVESVPRNSPEDHYCGLCGYGCRAGAKNGTDQTWLVD 447

Query: 480 LVRSGNGAILPGCEAIKIRQEKRDGSKCNRAKGI--VFEFGCGSAAKQMCLVESKVTIVA 539
            V +G   IL G +A +        S   R K    VF    G    +  ++E++VT+ +
Sbjct: 448 AVENG-AVILTGIKAERFVLVDNTSSSNERKKRCVGVFASSVGGKIGKKFIIEARVTVSS 507

Query: 540 CGALNTPPLLKRSGLRNPNIGKNLHLHPVTMAWGYFPAETDKSWPEKDKKSYEGGIMTAM 599
            G+L TPPL+  SGL+NPNIG+NL LHPV M WGYFP E D    E   K YEGGI+T++
Sbjct: 508 AGSLLTPPLMLSSGLKNPNIGRNLKLHPVLMTWGYFP-EKDS---EFSGKMYEGGIITSV 567

Query: 600 CNIGSEPDQEPGYGGAVIQTPALHPGLFSILMPWVSGTNIKHRMCKFSRTAHVFALARDK 659
            ++    D E G   A+++ P + P  ++ L PWVSG ++K RM K+ RTAH+FAL RD 
Sbjct: 568 HHMN---DTESGC-KAILENPLIGPASYAGLSPWVSGPDLKERMIKYGRTAHLFALVRDL 627

Query: 660 GSGTVKSSSSLSYKMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYR 719
           GSG V   + ++Y+    D ENL+ GL +AL++  AAGA E+GT+ + G+ +  + ++  
Sbjct: 628 GSGEVMMENEVTYRTTKKDRENLRAGLRQALRVSVAAGAVEVGTYRSDGQKMKCEAITKE 687

Query: 720 EYEKFVKEESS----RPLKDLLTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLFIA 779
             E+F+ E  +        +  T   SAHQMGSCRMG+ A++  +++ GE+WE EGLF+ 
Sbjct: 688 AMEEFLDEVDAVGGVGTKGEYWTTYFSAHQMGSCRMGVTAEEGALDENGESWEAEGLFVC 745

Query: 780 DSSVFPTALGVNPMVTVQAIAYCTAQSILETLKRK 807
           D S+ P+A+GVNPM+T+Q+ AYC +  I+++L+ K
Sbjct: 748 DGSILPSAVGVNPMITIQSTAYCISSKIVDSLQNK 745

BLAST of Bhi09G002289 vs. ExPASy TrEMBL
Match: A0A1S3BAP1 (Long-chain-alcohol oxidase OS=Cucumis melo OX=3656 GN=LOC103487628 PE=3 SV=1)

HSP 1 Score: 1444.5 bits (3738), Expect = 0.0e+00
Identity = 729/818 (89.12%), Postives = 760/818 (92.91%), Query Frame = 0

Query: 1   MGYFDTAASGGGSDIDDGQSNQQ-CQQCVIELHGGTDSFHDGASVGFSQHANSDD----L 60
           MGYF  AA+GGG   DDG++N Q    CVIELHGG DSFHDGASV  SQHANS++     
Sbjct: 12  MGYFCAAANGGGG--DDGETNHQPHHHCVIELHGGADSFHDGASVEISQHANSNNDYYSS 71

Query: 61  SSHLSAAEMESLAALCDTFLPSVAATAAADRSVTLFFETSASAAGTPERVGEMIKERVRH 120
           + HLSAAEMESLAALCDTFLPSVA   AADRS  +FFETSASA GTPERVGEMI ERVRH
Sbjct: 72  AGHLSAAEMESLAALCDTFLPSVAPATAADRSTAVFFETSASAVGTPERVGEMIMERVRH 131

Query: 121 PKKWLVRMTLWMLSTWYGTYILCGNESLSNHFPYFERFSKVSQKKREQIAISWSLSYFFL 180
           PKKWLVR++LWMLSTWYGTYILCGNESLSNHFPYF+RFSKVSQKKREQIA+SWSLSYFFL
Sbjct: 132 PKKWLVRVSLWMLSTWYGTYILCGNESLSNHFPYFKRFSKVSQKKREQIALSWSLSYFFL 191

Query: 181 LRMFFRTIKILCLLAFFTQVDEERNNISWKAIDYPGSDPQFKAKTKHSEASNLKEKGSKS 240
           LRMFFRTIKILCLLAFFTQVDEE NNISWKAIDYPGSDPQFK        SNLKEK SKS
Sbjct: 192 LRMFFRTIKILCLLAFFTQVDEEMNNISWKAIDYPGSDPQFK------PPSNLKEKDSKS 251

Query: 241 REIDQEDDE-----EELYGPLFKGIVNLQHQQQVQVAVGALRKFGFPVTLLSLSTKPPNP 300
           RE+D+E DE     EELYGPLFKGIVNLQHQQQ+QVAVGALRKFGFPVTLLS +TKPPNP
Sbjct: 252 REVDEEGDEEEEEAEELYGPLFKGIVNLQHQQQIQVAVGALRKFGFPVTLLSPTTKPPNP 311

Query: 301 SMIIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGPAMEEMYQAS 360
           SMIIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGPAMEEMYQ+S
Sbjct: 312 SMIIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGPAMEEMYQSS 371

Query: 361 GLVATDNMNVLILAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFRSNLYKEAMEV 420
           GLVATDNMNVLILAGSTVGGGSTVNWSASI+TPSHVT+EWSDEYELELFRSNLYKEA++V
Sbjct: 372 GLVATDNMNVLILAGSTVGGGSTVNWSASIKTPSHVTKEWSDEYELELFRSNLYKEAIDV 431

Query: 421 VCKKMGVQSEIDNEGFNNAVLRKGCEELGCHAMNIPRNSTSDHYCGWCCLGCKNGRKKGT 480
           VCKKMGVQS+IDNEGFNNAVLRKGCEELG HA+NIPRNSTSDHYCGWCCLGC++GRKKGT
Sbjct: 432 VCKKMGVQSKIDNEGFNNAVLRKGCEELGGHAINIPRNSTSDHYCGWCCLGCRDGRKKGT 491

Query: 481 AETWLVDLVRSGNGAILPGCEAIKIRQEKRDGSKCNRAKGIVFEFGCGSAAKQMCLVESK 540
           AETWLVDLVRSGNG ILPGCEAIKIRQEK+ G +   AKG+ FEF CGSAAKQMCLVESK
Sbjct: 492 AETWLVDLVRSGNGVILPGCEAIKIRQEKKQGRERKMAKGVAFEFWCGSAAKQMCLVESK 551

Query: 541 VTIVACGALNTPPLLKRSGLRNPNIGKNLHLHPVTMAWGYFPAETDKSWPEKDKKSYEGG 600
           VTIVACGALNTPPLLKRSGLRNPNIGKNLHLHPVTMAWGYFPAETDKSWPEKDKKSYEGG
Sbjct: 552 VTIVACGALNTPPLLKRSGLRNPNIGKNLHLHPVTMAWGYFPAETDKSWPEKDKKSYEGG 611

Query: 601 IMTAMCNIGSEPDQEPGYGGAVIQTPALHPGLFSILMPWVSGTNIKHRMCKFSRTAHVFA 660
           IMTAMCNIG EP+QEPGYGGAVIQTPALHPGLFSILMPWVSGTNIK RMCKFSRTAHVF 
Sbjct: 612 IMTAMCNIGPEPNQEPGYGGAVIQTPALHPGLFSILMPWVSGTNIKQRMCKFSRTAHVFV 671

Query: 661 LARDKGSGTVKSSSSLSYKMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVK 720
           LARDKGSGTVKS SSLSYKMD+VDE+NLQKGLEKALKILAAAGA EIGTHHNKGRSINVK
Sbjct: 672 LARDKGSGTVKSPSSLSYKMDNVDEQNLQKGLEKALKILAAAGAVEIGTHHNKGRSINVK 731

Query: 721 KVSYREYEKFVKEESSRPLKDLLTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLFI 780
           KVSYREYEKFVKEESSRPLKDLLTPICSAHQMGSCRMG GAKDSVVNQRGETWEVEGLFI
Sbjct: 732 KVSYREYEKFVKEESSRPLKDLLTPICSAHQMGSCRMGSGAKDSVVNQRGETWEVEGLFI 791

Query: 781 ADSSVFPTALGVNPMVTVQAIAYCTAQSILETLKRKRN 809
           ADSSVFPTALGVNPMVTVQAIAYCTAQS+LETLKRKRN
Sbjct: 792 ADSSVFPTALGVNPMVTVQAIAYCTAQSVLETLKRKRN 821

BLAST of Bhi09G002289 vs. ExPASy TrEMBL
Match: A0A6J1FFC0 (Long-chain-alcohol oxidase OS=Cucurbita moschata OX=3662 GN=LOC111443434 PE=3 SV=1)

HSP 1 Score: 1394.4 bits (3608), Expect = 0.0e+00
Identity = 712/808 (88.12%), Postives = 741/808 (91.71%), Query Frame = 0

Query: 1   MGYFDTAASGGGSDIDDGQSNQQCQQCVIELHGGTDSFHDGASVGFSQHANSDDLSSHLS 60
           MGYF  AAS GG    DG+S  Q  QCVIEL+GG DS HDG   G SQHAN    +  LS
Sbjct: 12  MGYFGAAASCGG----DGESKPQLHQCVIELNGGADSLHDGGPAGISQHAN---FNYSLS 71

Query: 61  AAEMESLAALCDTFLPSVAATAAADRSVTLFFETSASAAGTPERVGEMIKERVRHPKKWL 120
           AAEMESLAALCDTFLPSVAAT   D S + FFETSASAAGTPERVG MIKERVRHPKKWL
Sbjct: 72  AAEMESLAALCDTFLPSVAAT---DGSASEFFETSASAAGTPERVGGMIKERVRHPKKWL 131

Query: 121 VRMTLWMLSTWYGTYILCGNESLSNHFPYFERFSKVSQKKREQIAISWSLSYFFLLRMFF 180
           +R++LWMLSTWYGTYILCGN SLSNHFPYF+RFSKVSQKKREQ+ +SWSLSYF LLRMFF
Sbjct: 132 MRVSLWMLSTWYGTYILCGNGSLSNHFPYFQRFSKVSQKKREQVVVSWSLSYFLLLRMFF 191

Query: 181 RTIKILCLLAFFTQVDEERNNISWKAIDYPGSDPQFKAKTKHSEASNLKEKGSKSREIDQ 240
           RTIKILC LAFFTQVDEE NNISWKAIDYPGSDPQFKAK + SE  NLKEK  +SREI +
Sbjct: 192 RTIKILCHLAFFTQVDEEMNNISWKAIDYPGSDPQFKAKARQSEEWNLKEK-VRSREIVE 251

Query: 241 EDDEEELYGPLFKGIVNLQHQQQVQVAVGALRKFGFPVTLLSLSTKPPNPSMIIKCDAVV 300
           E  EEELYGPLFKGIVNL+HQ   QVAVGALRKFGFPVTLLS S K P+PSMIIKCDAVV
Sbjct: 252 E--EEELYGPLFKGIVNLEHQ---QVAVGALRKFGFPVTLLSPSIKSPSPSMIIKCDAVV 311

Query: 301 VGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGPAMEEMYQASGLVATDNMNV 360
           VGSGSGGGVVAGVLA+AGYKV+VLEKGSYCARTNLSLLEGPAMEEMY +SGLVATDNMNV
Sbjct: 312 VGSGSGGGVVAGVLASAGYKVVVLEKGSYCARTNLSLLEGPAMEEMYLSSGLVATDNMNV 371

Query: 361 LILAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFRSNLYKEAMEVVCKKMGVQSE 420
           LILAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELF SNLYKEA+EVVCK+MGVQSE
Sbjct: 372 LILAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFGSNLYKEALEVVCKRMGVQSE 431

Query: 421 IDNEGFNNAVLRKGCEELGCHAMNIPRNSTSDHYCGWCCLGCKNGRKKGTAETWLVDLVR 480
           IDNEGFNNAVLRKGCEELGC A+NIPRNS SDHYCGWCCLGCKNGRKKGTAETWLVDLVR
Sbjct: 432 IDNEGFNNAVLRKGCEELGCKAINIPRNSASDHYCGWCCLGCKNGRKKGTAETWLVDLVR 491

Query: 481 SGNGAILPGCEAIKIRQEKRDGSKCNRAKGIVFEFGCGSAAKQMCLVESKVTIVACGALN 540
           SGNGAILPGCEAI+I QEK DG K NRAKG+ FEFGCGS AKQMCLVESKVTIVACGAL 
Sbjct: 492 SGNGAILPGCEAIRITQEK-DGRKRNRAKGVAFEFGCGSGAKQMCLVESKVTIVACGALC 551

Query: 541 TPPLLKRSGLRNPNIGKNLHLHPVTMAWGYFPAETDKSWPEKDKKSYEGGIMTAMCNIGS 600
           TP LLKRSGLRNPNIGKNLHLHPVTMAWGYFPAETD+SWPEKDKKSYEGGIMTAMCNIGS
Sbjct: 552 TPQLLKRSGLRNPNIGKNLHLHPVTMAWGYFPAETDESWPEKDKKSYEGGIMTAMCNIGS 611

Query: 601 EPDQEPGYGGAVIQTPALHPGLFSILMPWVSGTNIKHRMCKFSRTAHVFALARDKGSGTV 660
            PDQEPGYGGAVIQTPALHPGLFSILMPWVSGTNIK RMCKFSRTAHVFALARDKGSGTV
Sbjct: 612 SPDQEPGYGGAVIQTPALHPGLFSILMPWVSGTNIKQRMCKFSRTAHVFALARDKGSGTV 671

Query: 661 KSSSSLSYKMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYREYEKF 720
           +SSSSLSY MDD DE+NLQKGLEKALKIL AAGAEEIGTHHNKGRSINVKKVSYREYE+F
Sbjct: 672 ESSSSLSYIMDDGDEKNLQKGLEKALKILGAAGAEEIGTHHNKGRSINVKKVSYREYERF 731

Query: 721 VKEESSRPLKDLLTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLFIADSSVFPTAL 780
           VKEESSRPLKDLLTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLFIADSSVFPTAL
Sbjct: 732 VKEESSRPLKDLLTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLFIADSSVFPTAL 791

Query: 781 GVNPMVTVQAIAYCTAQSILETLKRKRN 809
           GVNPMVTVQAIAYCTA+S+LETLKRKRN
Sbjct: 792 GVNPMVTVQAIAYCTAKSVLETLKRKRN 802

BLAST of Bhi09G002289 vs. ExPASy TrEMBL
Match: A0A6J1IPF0 (Long-chain-alcohol oxidase OS=Cucurbita maxima OX=3661 GN=LOC111477017 PE=3 SV=1)

HSP 1 Score: 1383.6 bits (3580), Expect = 0.0e+00
Identity = 706/808 (87.38%), Postives = 739/808 (91.46%), Query Frame = 0

Query: 1   MGYFDTAASGGGSDIDDGQSNQQCQQCVIELHGGTDSFHDGASVGFSQHANSDDLSSHLS 60
           MGYF  A+S GG    DG+S  Q  QCVIEL+G  DS HDG   G SQHANS   +  LS
Sbjct: 12  MGYFGAASSYGG----DGESKPQLHQCVIELNGCADSLHDGGPAGISQHANS---NYSLS 71

Query: 61  AAEMESLAALCDTFLPSVAATAAADRSVTLFFETSASAAGTPERVGEMIKERVRHPKKWL 120
           AAEMESLAALC+TFLPSVAAT   D S + FFETSASAAGT ERVG MIKERVRHPKKWL
Sbjct: 72  AAEMESLAALCETFLPSVAAT---DGSASEFFETSASAAGTSERVGGMIKERVRHPKKWL 131

Query: 121 VRMTLWMLSTWYGTYILCGNESLSNHFPYFERFSKVSQKKREQIAISWSLSYFFLLRMFF 180
           +RM+LWMLSTWYGTYILCGN SLSNHFPYF+RFSKVSQKKREQ+ +SWSLSYF LLRMFF
Sbjct: 132 MRMSLWMLSTWYGTYILCGNGSLSNHFPYFQRFSKVSQKKREQVVVSWSLSYFLLLRMFF 191

Query: 181 RTIKILCLLAFFTQVDEERNNISWKAIDYPGSDPQFKAKTKHSEASNLKEKGSKSREIDQ 240
           RTIKIL  LAFFTQVDEE NNISWKAIDYPGSDPQFKAK + SE  NLKEK  +SREI  
Sbjct: 192 RTIKILSHLAFFTQVDEEMNNISWKAIDYPGSDPQFKAKARQSEEWNLKEK-VRSREI-- 251

Query: 241 EDDEEELYGPLFKGIVNLQHQQQVQVAVGALRKFGFPVTLLSLSTKPPNPSMIIKCDAVV 300
           E++EEELYGPLFKGIVNL+HQ    VAVGALRKFGFPVTLLS STK P+PSMIIKCDAVV
Sbjct: 252 EEEEEELYGPLFKGIVNLEHQ---VVAVGALRKFGFPVTLLSPSTKSPSPSMIIKCDAVV 311

Query: 301 VGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGPAMEEMYQASGLVATDNMNV 360
           VGSGSGGGVV GVLA+AGYKV+VLEKGSYCARTNLSLLEGPAMEEMY +SGLVATDNMNV
Sbjct: 312 VGSGSGGGVVGGVLASAGYKVVVLEKGSYCARTNLSLLEGPAMEEMYLSSGLVATDNMNV 371

Query: 361 LILAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFRSNLYKEAMEVVCKKMGVQSE 420
           LILAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFRSNLYKEA+EVVCK+MGVQSE
Sbjct: 372 LILAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFRSNLYKEALEVVCKRMGVQSE 431

Query: 421 IDNEGFNNAVLRKGCEELGCHAMNIPRNSTSDHYCGWCCLGCKNGRKKGTAETWLVDLVR 480
           IDNEGFNNA+LRKGCEELGC A+NIPRNS SDHYCGWCCLGCKNGRKKGTAETWLVDLVR
Sbjct: 432 IDNEGFNNAILRKGCEELGCKAINIPRNSASDHYCGWCCLGCKNGRKKGTAETWLVDLVR 491

Query: 481 SGNGAILPGCEAIKIRQEKRDGSKCNRAKGIVFEFGCGSAAKQMCLVESKVTIVACGALN 540
           SGNGAILPGCEAI+I QEK DG + NRAKG+ FEF CGS AKQMCLVESKVTIVACGAL 
Sbjct: 492 SGNGAILPGCEAIRITQEK-DGRERNRAKGVAFEFECGSEAKQMCLVESKVTIVACGALC 551

Query: 541 TPPLLKRSGLRNPNIGKNLHLHPVTMAWGYFPAETDKSWPEKDKKSYEGGIMTAMCNIGS 600
           TPPLLKRSGLRNPNIGKNLHLHPV MAWGYFPAETD+SWPEKDKKSYEGGIMTAMCNIGS
Sbjct: 552 TPPLLKRSGLRNPNIGKNLHLHPVAMAWGYFPAETDESWPEKDKKSYEGGIMTAMCNIGS 611

Query: 601 EPDQEPGYGGAVIQTPALHPGLFSILMPWVSGTNIKHRMCKFSRTAHVFALARDKGSGTV 660
            PDQEPGYGGAVIQTPALHPGLFSILMPWVSGTNIK RMCKFSRTAHVFALARDKGSGTV
Sbjct: 612 SPDQEPGYGGAVIQTPALHPGLFSILMPWVSGTNIKQRMCKFSRTAHVFALARDKGSGTV 671

Query: 661 KSSSSLSYKMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSINVKKVSYREYEKF 720
           +SSSSLSY MDD DE+NLQKGLEKALKIL AAGAEEIGTHHNKGRSINVKKVSYREYE+F
Sbjct: 672 ESSSSLSYIMDDGDEKNLQKGLEKALKILGAAGAEEIGTHHNKGRSINVKKVSYREYERF 731

Query: 721 VKEESSRPLKDLLTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLFIADSSVFPTAL 780
           VKEESSRPLKDLLTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLF+ADSSVFPTAL
Sbjct: 732 VKEESSRPLKDLLTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGLFVADSSVFPTAL 791

Query: 781 GVNPMVTVQAIAYCTAQSILETLKRKRN 809
           GVNPMVTVQAIAYCTAQS+LETLKRKRN
Sbjct: 792 GVNPMVTVQAIAYCTAQSVLETLKRKRN 802

BLAST of Bhi09G002289 vs. ExPASy TrEMBL
Match: A0A6J1FQT3 (Long-chain-alcohol oxidase OS=Cucurbita moschata OX=3662 GN=LOC111446403 PE=3 SV=1)

HSP 1 Score: 1300.4 bits (3364), Expect = 0.0e+00
Identity = 667/821 (81.24%), Postives = 718/821 (87.45%), Query Frame = 0

Query: 1   MGYFDTAAS------------GGGSDIDDGQSNQQCQQCVIELHGGTDSFHDGASVGFSQ 60
           MGYF  AA+            GGG    D ++ ++    VIEL       HDGA     +
Sbjct: 32  MGYFGAAAAAEEVEVEVESCCGGGG--GDDEAKRKLDHYVIEL-------HDGAPAEILE 91

Query: 61  HANSDDLSSHLSAAEMESLAALCDTFLPSVAATAAADRSVTLFFETSASAAGTPERVGEM 120
           HANS++  S LSAAEMESLAALCDTFLPSV     AD S  +FFETSA+AAGTPE+VG+M
Sbjct: 92  HANSNNNCS-LSAAEMESLAALCDTFLPSV----TADGSAAMFFETSAAAAGTPEKVGKM 151

Query: 121 IKERVRHPKKWLVRMTLWMLSTWYGTYILCGNESLSNHFPYFERFSKVSQKKREQIAISW 180
           IKERV+HPKKWLVR++LWMLSTWYGTYILCGN SLS++FPYF+ F K+SQ KREQI +SW
Sbjct: 152 IKERVKHPKKWLVRVSLWMLSTWYGTYILCGNRSLSHNFPYFQTFPKLSQTKREQIVVSW 211

Query: 181 SLSYFFLLRMFFRTIKILCLLAFFTQVDEERNNISWKAIDYPGSDPQFKAKTKHSEASNL 240
           SLSYFFLLRM+FRTIKI CLL FFTQVD++ NNISW+AIDYPG+DPQFKAKTK  EA   
Sbjct: 212 SLSYFFLLRMWFRTIKIFCLLTFFTQVDDKTNNISWQAIDYPGADPQFKAKTKQLEA--- 271

Query: 241 KEKGSKSREIDQEDDEEELYGPLFKGIVNLQHQQQVQVAVG-ALRKFGFPVTLLSLSTKP 300
                 SRE+  E+DEEELYGPLFKGIV+L +QQ    A   ALRKFGFPVTLLS   K 
Sbjct: 272 ------SREV--EEDEEELYGPLFKGIVHLDNQQVAAAAAAEALRKFGFPVTLLSPMIKA 331

Query: 301 PNPSMIIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGPAMEEMY 360
            +PSMIIKCDAVVVGSGSGGGVVAGVLANAGYKV+VLEKGSYCARTNLSLLEGPAMEEMY
Sbjct: 332 SSPSMIIKCDAVVVGSGSGGGVVAGVLANAGYKVVVLEKGSYCARTNLSLLEGPAMEEMY 391

Query: 361 QASGLVATDNMNVLILAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFRSNLYKEA 420
            +SGLVATDNMNVL+LAGSTVGGGSTVNWSASIQTPSHVTREWSD+YELELF SNLYKEA
Sbjct: 392 LSSGLVATDNMNVLVLAGSTVGGGSTVNWSASIQTPSHVTREWSDKYELELFSSNLYKEA 451

Query: 421 MEVVCKKMGVQSEIDNEGFNNAVLRKGCEELGCHAMNIPRNSTSDHYCGWCCLGCKNGRK 480
           MEVVCKKMGVQSEIDNEGFNNAVLRKGCEELG HA+NIPRNSTSDHYCGWCCLGCK+GRK
Sbjct: 452 MEVVCKKMGVQSEIDNEGFNNAVLRKGCEELGYHAINIPRNSTSDHYCGWCCLGCKDGRK 511

Query: 481 KGTAETWLVDLVRSGNGAILPGCEAIKIRQEKRDGSKCNRAKGIVFEFGCGSAAKQMCLV 540
           KGTAETWLVDLVRSGNG ILPGCEAI IR+EK+DGS+ NRAKGI FEFGCGS  KQ+CLV
Sbjct: 512 KGTAETWLVDLVRSGNGVILPGCEAIDIRKEKKDGSERNRAKGIAFEFGCGSGVKQVCLV 571

Query: 541 ESKVTIVACGALNTPPLLKRSGLRNPNIGKNLHLHPVTMAWGYFPAETDKSWPEKDKKSY 600
           ESKVTIVACGAL+TPPLL RSGLRNPNIGKNLHLHPVTMAWG+FPAETDK WPE+ KKSY
Sbjct: 572 ESKVTIVACGALSTPPLLLRSGLRNPNIGKNLHLHPVTMAWGHFPAETDKPWPEEHKKSY 631

Query: 601 EGGIMTAMCNIGSEPDQEPGYGGAVIQTPALHPGLFSILMPWVSGTNIKHRMCKFSRTAH 660
           EGGIMTAMCNI SEPDQEPG GGAVIQTPALHPGLFSILMPW+SGTNIK RM KFSRTAH
Sbjct: 632 EGGIMTAMCNIRSEPDQEPGSGGAVIQTPALHPGLFSILMPWLSGTNIKQRMRKFSRTAH 691

Query: 661 VFALARDKGSGTVKSSSSLSYKMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSI 720
           VF LARDKGSGTVKS + +SY M++VDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSI
Sbjct: 692 VFVLARDKGSGTVKSPNCISYNMEEVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSI 751

Query: 721 NVKKVSYREYEKFVKEESSRPLKDLLTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEG 780
           NVKKVSYREYEKFVKEESSR LKDL TPICSAHQMGSCRMGIGA+DSVV+QRGETWEVEG
Sbjct: 752 NVKKVSYREYEKFVKEESSRALKDLKTPICSAHQMGSCRMGIGARDSVVDQRGETWEVEG 811

Query: 781 LFIADSSVFPTALGVNPMVTVQAIAYCTAQSILETLKRKRN 809
           LFIADSSVFPTALGVNPMVTVQAIAYCTAQS+LETLKRKRN
Sbjct: 812 LFIADSSVFPTALGVNPMVTVQAIAYCTAQSVLETLKRKRN 827

BLAST of Bhi09G002289 vs. ExPASy TrEMBL
Match: A0A6J1CNU3 (Long-chain-alcohol oxidase OS=Momordica charantia OX=3673 GN=LOC111012719 PE=3 SV=1)

HSP 1 Score: 1288.5 bits (3333), Expect = 0.0e+00
Identity = 658/820 (80.24%), Postives = 714/820 (87.07%), Query Frame = 0

Query: 1   MGY--FDTAASGGGSDIDDGQSNQQCQQCVIELHGGTDSFHDGASVGFSQHANSDDLSSH 60
           MGY     AAS GG    DG   Q  + C++ELHGG D+  +G+ V   QHA     +S 
Sbjct: 1   MGYCGATAAASSGG----DGDGEQHRRHCIVELHGGADTILNGSPVQNPQHAKYSHNNS- 60

Query: 61  LSAAEMESLAALCDTFLPSVAAT--------AAADRSVTLFFETSASAAGTPERVGEMIK 120
           L+AAEMESLAALCDTFLPSVAA         ++A  +   FF+TSASAAGTPERVG MI 
Sbjct: 61  LTAAEMESLAALCDTFLPSVAAATSGHSNFESSAATATAAFFQTSASAAGTPERVGGMIS 120

Query: 121 ERVRHPKKWLVRMTLWMLSTWYGTYILCGNESLSNHFPYFERFSKVSQKKREQIAISWSL 180
           ERVRHPKKW+VR++LWMLSTWYGTYILCGN SLSNHFPYF+ FSK+S  KRE+IA+SWSL
Sbjct: 121 ERVRHPKKWMVRLSLWMLSTWYGTYILCGNASLSNHFPYFQTFSKLSHNKREKIALSWSL 180

Query: 181 SYFFLLRMFFRTIKILCLLAFFTQVDEERNNISWKAIDYPGSDPQFKAKTKHSEASNLKE 240
           S+F L RMFFRTIKIL LLAFF+QVDE+ NNISWKAIDYPG DPQFK +T+ SEASNL E
Sbjct: 181 SFFLLHRMFFRTIKILSLLAFFSQVDEKMNNISWKAIDYPGPDPQFKLETRQSEASNLNE 240

Query: 241 KGSKSREIDQEDDEEELYGPLFKGIVNLQHQQQVQVAVGALRKFGFPVTLLSLSTKPPNP 300
            G +SR       EEELYGPLF+GIVN+   Q+  VA  ALRKFGFPV  L   TK  +P
Sbjct: 241 NG-RSR-------EEELYGPLFRGIVNI--DQRRGVAAEALRKFGFPVAFLPPRTKTASP 300

Query: 301 SMIIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGPAMEEMYQAS 360
           SM+IKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEG AMEEMY +S
Sbjct: 301 SMMIKCDAVVVGSGSGGGVVAGVLANAGYKVLVLEKGSYCARTNLSLLEGQAMEEMYLSS 360

Query: 361 GLVATDNMNVLILAGSTVGGGSTVNWSASIQTPSHVTREWSDEYELELFRSNLYKEAMEV 420
           GL+ATDNMNVL+LAGSTVGGGSTVNWSAS QTP+HVTREWSD YEL+LF SNLYKEAMEV
Sbjct: 361 GLLATDNMNVLVLAGSTVGGGSTVNWSASFQTPTHVTREWSDVYELDLFSSNLYKEAMEV 420

Query: 421 VCKKMGVQSEIDNEGFNNAVLRKGCEELGCHAMNIPRNSTSDHYCGWCCLGCKNGRKKGT 480
           VCKKMGVQSEI++EGFNNAVLRKGCEELG HA+NIPRNS +DHYCGWCCLGCKNGRKKGT
Sbjct: 421 VCKKMGVQSEIESEGFNNAVLRKGCEELGYHAINIPRNSPADHYCGWCCLGCKNGRKKGT 480

Query: 481 AETWLVDLVRSGNGAILPGCEAIKIRQEKRDGSKCNRAKGIVFEFGCGSAAKQMCLVESK 540
           AETWLVDLVRSGNGAILPGCEA++I  EK+DGS+  RAKGI FEFGC S AKQMCLVESK
Sbjct: 481 AETWLVDLVRSGNGAILPGCEAVRIMWEKKDGSERKRAKGITFEFGCQSGAKQMCLVESK 540

Query: 541 VTIVACGALNTPPLLKRSGLRNPNIGKNLHLHPVTMAWGYFPAET--DKSWPEKDKKSYE 600
           VTIVACGAL+TPPLLKRSGLRNPNIGK+LHLHPVTMAWG+FPAET  DK+WPE+ KKSYE
Sbjct: 541 VTIVACGALSTPPLLKRSGLRNPNIGKHLHLHPVTMAWGHFPAETDNDKAWPEEGKKSYE 600

Query: 601 GGIMTAMCNIGSEPDQEPGYGGAVIQTPALHPGLFSILMPWVSGTNIKHRMCKFSRTAHV 660
           GGIMTAMCNIG+E D EPGYGGAVIQTPALHPGLFSILMPWVSGTN KHRMCKFSRTAHV
Sbjct: 601 GGIMTAMCNIGTEADNEPGYGGAVIQTPALHPGLFSILMPWVSGTNFKHRMCKFSRTAHV 660

Query: 661 FALARDKGSGTVKSSSSLSYKMDDVDEENLQKGLEKALKILAAAGAEEIGTHHNKGRSIN 720
           FALARDKGSGTV+SSSS+SYKMD  DEENLQKG+EKAL+ILAAAGAEEIGTHHNKGRSI 
Sbjct: 661 FALARDKGSGTVRSSSSISYKMDGADEENLQKGVEKALRILAAAGAEEIGTHHNKGRSIK 720

Query: 721 VKKVSYREYEKFVKEESSRPLKDLLTPICSAHQMGSCRMGIGAKDSVVNQRGETWEVEGL 780
           VKKVSYREYEKFVKEESSRPL DL TPICSAHQMGSCRMGIG KDSVVNQRGETWE+EGL
Sbjct: 721 VKKVSYREYEKFVKEESSRPLNDLATPICSAHQMGSCRMGIGPKDSVVNQRGETWEMEGL 780

Query: 781 FIADSSVFPTALGVNPMVTVQAIAYCTAQSILETLKRKRN 809
           FIADSSVFPTALGVNPMVTVQAIAYCTAQS+L+TLKRKRN
Sbjct: 781 FIADSSVFPTALGVNPMVTVQAIAYCTAQSVLDTLKRKRN 805